keyword
MENU ▼
Read by QxMD icon Read
search

swine infection

keyword
https://www.readbyqxmd.com/read/29333678/supplementary-feeding-as-a-source-of-multiresistant-salmonella-in-endangered-egyptian-vultures
#1
Guillermo Blanco
Wild birds have repeatedly been highlighted as vectors in the dissemination of livestock and human pathogens. Here, the occurrence, serotypes and antimicrobial resistance of Salmonella were assessed in adult Egyptian vultures (Neophron percnopterus), to test the hypothesis that infection is associated with the consumption of swine carcasses provided at supplementary feeding stations (SFSs). Faeces of year-round resident griffon vultures (Gyps fulvus) were also tested to assess whether infection was acquired in the breeding grounds of both species or in the African wintering quarters of Egyptian vultures...
January 14, 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29330937/first-time-detection-of-porcine-reproductive-and-respiratory-syndrome-virus-prrsv-infection-in-uruguay
#2
N Ramos, S Mirazo, G Castro, K Cabrera, F Osorio, J Arbiza
Within the last two decades, several high-impact viruses have emerged in the global swine population, including porcine reproductive and respiratory syndrome virus (PRRSV). In Uruguay, the more recent serological survey for PRRSV and other notifiable diseases such as Aujeszky's disease virus (ADV) and classical swine fever virus (CSFV) dated from year 2000. The main purpose of this study was to update our information on the infection status of PRRSV, ADV and CSFV in Uruguayan pig herds, in order to keep informed about the epidemiological situation of these notifiable infections in the country...
January 12, 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29327672/linear-epitopes-in-african-swine-fever-virus-p72-recognized-by-monoclonal-antibodies-prepared-against-baculovirus-expressed-antigen
#3
Mallory E Heimerman, Maria V Murgia, Ping Wu, Andre D Lowe, Wei Jia, Raymond R Rowland
Protein p72 is the major capsid protein of African swine fever virus (ASFV) and is an important target for test and vaccine development. Monoclonal antibodies (mAbs) were prepared against a recombinant antigenic fragment, from amino acid (aa) 20-303, expressed in baculovirus. A total of 29 mAbs were recovered and tested by immunofluorescent antibody (IFA) staining on ASFV Lisbon-infected Vero cells. Six antibodies were IFA-positive and selected for further characterization. Epitope mapping was performed against overlapping polypeptides expressed in E...
January 1, 2018: Journal of Veterinary Diagnostic Investigation
https://www.readbyqxmd.com/read/29327614/intracellular-african-swine-fever-virus-dna-remains-unmethylated-in-infected-vero-cells
#4
Stefanie Weber, Astghik Hakobyan, Hovakim Zakaryan, Walter Doerfler
AIM: Sequence-specific CpG methylation of eukaryotic promoters is an important epigenetic signal for long-term gene silencing. We have now studied the methylation status of African swine fever virus (ASFV) DNA at various times after infection of Vero cells in culture. METHODS & RESULTS: ASFV DNA was detectable throughout the infection cycle and was found unmethylated in productively infected Vero cells as documented by bisulfite sequencing of 13 viral DNA segments...
January 12, 2018: Epigenomics
https://www.readbyqxmd.com/read/29324680/phylogenetic-analysis-and-characterization-of-a-sporadic-isolate-of-equine-influenza-a-h3n8-from-an-unvaccinated-horse-in-2015
#5
Chithra C Sreenivasan, Sunayana S Jandhyala, Sisi Luo, Ben M Hause, Milton Thomas, David E B Knudsen, Pamela Leslie-Steen, Travis Clement, Stephanie E Reedy, Thomas M Chambers, Jane Christopher-Hennings, Eric Nelson, Dan Wang, Radhey S Kaushik, Feng Li
Equine influenza, caused by the H3N8 subtype, is a highly contagious respiratory disease affecting equid populations worldwide and has led to serious epidemics and transboundary pandemics. This study describes the phylogenetic characterization and replication kinetics of recently-isolated H3N8 virus from a nasal swab obtained from a sporadic case of natural infection in an unvaccinated horse from Montana, USA. The nasal swab tested positive for equine influenza by Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction (RT-PCR)...
January 11, 2018: Viruses
https://www.readbyqxmd.com/read/29324236/irf1-up-regulates-isg15-gene-expression-in-dsrna-stimulation-or-csfv-infection-by-targeting-nucleotides-487-to-325-in-the-5-flanking-region
#6
Xiao-Quan Li, Xiao Ning Li, Jing-Jing Liang, Xin-Bin Cai, Qian Tao, Yu-Xiao Li, Qing Qin, Su-Ping Xu, Ting Rong Luo
Interferon (IFN)-stimulated gene 15 (ISG15) encodes a ubiquitin-like protein that is heavily involved in immune response elicitation. As an important member of interferon regulatory factor (IRF) family, IRF1 can activate the expression of multiple genes, including the human optineurin gene (Sudhakar et al., 2013). In this study, a sequence in the promoter region of the optineurin gene was compared to the 5' flanking region of the porcine isg15 gene. Porcine IRF1 also possesses antiviral activity against several swine viruses (Li et al...
January 8, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29317201/mutation-induced-changes-of-transmembrane-pore-size-revealed-by-combined-ion-channel-conductance-and-single-vesicle-permeabilization-analyses
#7
Eneko Largo, Douglas P Gladue, Johana Torralba, Vicente M Aguilella, Antonio Alcaraz, Manuel V Borca, José L Nieva
Permeabilization of the Endoplasmic Reticulum (ER) is instrumental in the progression of host-cell infection by many viral pathogens. We have described that permeabilization of ER model membranes by the pore-forming domain of the Classical Swine Fever Virus (CSFV) p7 protein depends on two sequence determinants: the C-terminal transmembrane helix, and the preceding polar loop that regulates its activity. Here, by combining ion-channel activity measurements in planar lipid bilayers with imaging of single Giant Unilamellar Vesicles (GUVs), we demonstrate that point substitutions directed to conserved residues within these regions affect ER-like membrane permeabilization following distinct mechanisms...
January 6, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29316958/molecular-subtyping-of-european-swine-influenza-viruses-and-scaling-to-high-throughput-analysis
#8
Emilie Bonin, Stéphane Quéguiner, Cédric Woudstra, Stéphane Gorin, Nicolas Barbier, Timm C Harder, Patrick Fach, Séverine Hervé, Gaëlle Simon
BACKGROUND: Swine influenza is a respiratory infection of pigs that may have a significant economic impact in affected herds and pose a threat to the human population since swine influenza A viruses (swIAVs) are zoonotic pathogens. Due to the increasing genetic diversity of swIAVs and because novel reassortants or variants may become enzootic or have zoonotic implications, surveillance is strongly encouraged. Therefore, diagnostic tests and advanced technologies able to identify the circulating strains rapidly are critically important...
January 10, 2018: Virology Journal
https://www.readbyqxmd.com/read/29314759/reduced-specificity-of-erns-antibody-elisas-for-samples-from-piglets-with-maternally-derived-antibodies-induced-by-vaccination-of-sows-with-classical-swine-fever-marker-vaccine-cp7_e2alf
#9
D Meyer, W Loeffen, A Postel, S Fritsche, P Becher
Successful implementation of marker vaccines against classical swine fever virus is dependent on a reliable accompanying diagnostic assay that allows differentiation of infected from vaccinated animals (DIVA) as well as the development of a testing scheme during emergency vaccination. In this context, special attention needs to be paid to breeding farms, because the offspring of marker vaccinated sows possess maternally derived antibodies (MDAs). So far, limited information is available on the influence of MDAs on serological testing in the context of a DIVA strategy...
January 4, 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29314736/development-of-a-chlamydia-suis-specific-antibody-enzyme-linked-immunosorbent-assay-based-on-the-use-of-a-b-cell-epitope-of-the-polymorphic-membrane-protein-c
#10
K De Puysseleyr, E Kieckens, L De Puysseleyr, H Van den Wyngaert, B Ahmed, S Van Lent, H H Creasy, G S A Myers, D Vanrompay
Chlamydia suis infections lead to economic loss in the pork industry. Chlamydia suis infections could be successfully treated with tetracyclines until the appearance of a tetracycline resistant phenotype, which was acquired via horizontal gene transfer of the tet(C) gene. Given the importance of C. suis as a swine pathogen and as a recently emerged tetracycline resistant pathogen with zoonotic potential, our aim was to develop a sensitive C. suis-specific antibody ELISA based on the polymorphic membrane proteins (Pmps)...
January 4, 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29311002/humoral-and-cellular-immune-response-in-mice-induced-by-the-classical-swine-fever-virus-e2-protein-fused-to-the-porcine-cd154-antigen
#11
Yusmel Sordo, Marisela Suárez, Rosalina Caraballo, Talía Sardina, Emma Brown, Carlos Duarte, Joanna Lugo, Lázaro Gil, Danny Perez, Ayme Oliva, Milagros Vargas, Elaine Santana, Rodolfo Valdés, María Pilar Rodríguez
The development of subunit vaccines against classical swine fever is a desirable goal, because it allows discrimination between vaccinated and infected animals. In this study, humoral and cellular immune response elicited in inbred BALB/c mice by immunization with a recombinant classical swine fever virus (CSFV) E2 protein fused to porcine CD154 antigen (E2CD154) was assessed. This model was used as a predictor of immune response in swine. Mice were immunized with E2CD154 emulsified in Montanide ISA50V2 or dissolved in saline on days 1 and 21...
January 5, 2018: Biologicals: Journal of the International Association of Biological Standardization
https://www.readbyqxmd.com/read/29307051/complete-genome-sequence-of-a-novel-t7-like-bacteriophage-from-a-pasteurella-multocida-capsular-type-a-isolate
#12
Yibao Chen, Erchao Sun, Jiaoyang Song, Lan Yang, Bin Wu
A novel virulent bacteriophage, vB_PmuP_PHB02 (phage PHB02), infecting Pasteurella multocida capsular type A strains, was isolated from wastewater from a swine farm in China. Phage PHB02 has a linear double-stranded DNA genome consisting of 38,670 base pairs (bp), with a G+C content of 40.8% and a 127-bp terminal redundancy. Forty-eight putative open reading frames were identified, and no transfer RNA-encoding genes were detected. The morphology and genomic structure of phage PHB02 resemble those of T7-like phages belonging to the family Podoviridae, of the order Caudovirales...
January 6, 2018: Current Microbiology
https://www.readbyqxmd.com/read/29306502/identification-and-characterization-of-a-novel-protective-antigen-sec_205-of-streptococcus-equi-ssp-zooepidemicus
#13
Huihuang Liang, Bin Tang, Pengpeng Zhao, Mingyong Deng, Lili Yan, Pan Zhai, Zigong Wei
Streptococcus equi ssp. zooepidemicus (SEZ) is an important pathogen of swine streptococcal diseases and can infect a wide range of animals as well as human beings. The absence of effective vaccine confounds the control of SEZ infection. Sec_205, a novel protein identified in the previous study, was inducibly over-expressed in Escherichia coli in the present study. The purified recombinant protein could elicit a significant humoral antibody response and provide efficient protection against lethal challenge of SEZ C55138 in mouse model...
January 3, 2018: Vaccine
https://www.readbyqxmd.com/read/29306352/porcine-commensal-escherichia-coli-a-reservoir-for-class-1-integrons-associated-with-is26
#14
Cameron J Reid, Ethan R Wyrsch, Piklu Roy Chowdhury, Tiziana Zingali, Michael Liu, Aaron E Darling, Toni A Chapman, Steven P Djordjevic
Porcine faecal waste is a serious environmental pollutant. Carriage of antimicrobial-resistance genes (ARGs) and virulence-associated genes (VAGs), and the zoonotic potential of commensal Escherichia coli from swine are largely unknown. Furthermore, little is known about the role of commensal E. coli as contributors to the mobilization of ARGs between food animals and the environment. Here, we report whole-genome sequence analysis of 103 class 1 integron-positive E. coli from the faeces of healthy pigs from two commercial production facilities in New South Wales, Australia...
December 2017: Microbial Genomics
https://www.readbyqxmd.com/read/29300158/vaccination-of-sows-with-a-dendritic-cell-targeted-porcine-epidemic-diarrhea-virus-s1-protein-based-candidate-vaccine-reduced-viral-shedding-but-exacerbated-gross-pathological-lesions-in-suckling-neonatal-piglets
#15
Sakthivel Subramaniam, Danielle M Yugo, C Lynn Heffron, Adam J Rogers, Harini Sooryanarain, Tanya LeRoith, Christopher Overend, Dianjun Cao, Xiang-Jin Meng
Porcine epidemic diarrhea virus (PEDV) poses a serious threat to swine worldwide as evidenced by its recent introduction into the USA and the devastating economic impact it caused to the USA swine industry. Commercial vaccines against PEDV are available but their efficacies are inadequate. Therefore, vaccines with improved efficacy are needed to effectively control PEDV infections. We previously determined the immunogenicity of a novel dendritic cell (DC)-targeted PEDV S1 protein-based subunit vaccine in weaned piglets in which the PEDV antigen was targeted to DCs through a porcine Langerin-specific antibody...
January 4, 2018: Journal of General Virology
https://www.readbyqxmd.com/read/29299685/toxoplasma-gondii-infection-and-biosecurity-levels-in-fattening-pigs-and-sows-serological-and-molecular-epidemiology-in-the-intensive-pig-industry-lombardy-northern-italy
#16
Alessia Libera Gazzonis, Marianna Marangi, Luca Villa, Maria Elena Ragona, Emanuela Olivieri, Sergio Aurelio Zanzani, Annunziata Giangaspero, Maria Teresa Manfredi
Toxoplasma gondii is a widespread protozoan affecting animals and humans. One of the major routes of human infection is through the consumption of raw or under-cooked meat, particularly of certain animal species, including pigs. Although T. gondii represents an important public health issue, its control at slaughter is not mandatory. Consequently, available information on T. gondii infection in domestic animals destined for human consumption is scarce. Thus, an epidemiologic survey was designed to update information on T...
January 3, 2018: Parasitology Research
https://www.readbyqxmd.com/read/29298122/evaluation-of-viral-genome-copies-within-viral-factories-on-different-dna-viruses
#17
Zaven A Karalyan, Roza A Izmailyan, Liana O Abroyan, Aida S Avetisyan, Lina A Hakobyan, Hovakim S Zakaryan, Elena M Karalova
This article describes a simple method of measuring the number of viral genomes within viral factories. For this purpose, we use three DNA viruses replicating in the cytoplasm of the infected cells: wild-type African swine fever virus (ASFV)-Georgia 2007, culture-adapted type ASFV-BA71V, and Vaccinia virus (VV). The measurements are conducted in three steps. In the first step, after DNA staining, we evaluate Integrated Optical Density (IOD) of total DNA for each viral factory. The second step involves the calculations of the mass of DNA in the viral factories in picograms (pg)...
January 1, 2018: Journal of Histochemistry and Cytochemistry: Official Journal of the Histochemistry Society
https://www.readbyqxmd.com/read/29290292/mathematical-modeling-of-porcine-epidemic-diarrhea-virus-dynamics-within-a-farrow-to-finish-swine-farm-to-investigate-the-effects-of-control-measures
#18
Kiyokazu Murai, Sachiko Moriguchi, Yoko Hayama, Sota Kobayashi, Ayako Miyazaki, Toshiyuki Tsutsui, Takehisa Yamamoto
Porcine epidemic diarrhea (PED) is a highly contagious enteric disease in swine that can cause devastating economic damage to pig producers. Japan was severely affected by PED epidemics from 2013 through 2015, with over 1000 farms were affected during this period. Although many studies have unraveled pathological and molecular characteristics of PED virus (PEDV), the mechanism for within-farm spread is largely unknown. Here, we constructed a deterministic compartmental model to quantitatively describe the infection dynamics in a farm setting and to investigate effective control measures...
January 1, 2018: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/29285901/characterization-of-porcine-sapelovirus-isolated-from-japanese-swine-with-plc-prf-5-cells
#19
H Bai, J Liu, L Fang, M Kataoka, N Takeda, T Wakita, T-C Li
Porcine sapelovirus (PSV) is a causative agent of neurological disorders, fertility disorders and dermal lesions of swine. In this study, we isolated two PSV strains, Jpsv477 and Jpsv1315, from swine faecal specimens using a PLC/PRF/5 cell culture system. The PSV infection of PLC/PRF/5 cells induced a cytopathic effect (CPE). Two types of virus particles with identical diameter (~35 nm) but different densities (1.300 and 1.285 g/cm3 ) were observed in the cell culture supernatants. Analysis of the entire genome sequence of Jpsv477 and Jpsv1315 revealed that both strains possess 7,558 nucleotides and the poly (A) tail and have a typical PSV genome organization consisting of a 5' terminal untranslated region (5'UTR), a large open reading frame (ORF), and a 3' terminal untranslated region (3'UTR)...
December 28, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29284213/pathologic-and-molecular-characterization-of-streptococcus-dysgalactiae-subspecies-equisimilis-infection-in-neonatal-piglets
#20
Sang-Ik Oh, Jong Wan Kim, Ji-Youl Jung, Myeongju Chae, Yu-Ran Lee, Jong Ho Kim, ByungJae So, Ha-Young Kim
Streptococcus dysgalactiae subspecies equisimilis (SDSE) is an emerging pathogen in animals and humans. Here, we describe two clinical swine cases of SDSE infection presenting with lameness, neurological signs, or sudden death. Pathological examination indicated suppurative arthritis, encephalitis, and multifocal abscesses in the kidney and heart. β-hemolytic colonies from the joint samples of each case were identified as SDSE. The isolates showed low minimum inhibitory concentration values for β-lactams and presented the same virulence gene profile (slo-/sagA+/pSTKP8+)...
December 28, 2017: Journal of Veterinary Science
keyword
keyword
78849
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"