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https://www.readbyqxmd.com/read/28219820/versatile-carboxyl-terminus-of-capsid-protein-of-porcine-circovirus-type-2-were-recognized-by-monoclonal-antibodies-with-pluripotency-of-binding
#1
Ling-Chu Hung, Ivan-Chen Cheng
We designed the peptide (C3) mimetic carboxyl-terminus (Cterminus) of capsid protein of porcine circovirus type 2b (PCV2b-1A/1B) inducing humoral immunity and generating hybridomas. The positive reactivity of the mAbs to PCV2 capsid protein was demonstrated by Western blot assay. Those mAbs also showed positive signals on PCV2b infected swine lymphocytes by indirect immunofluorescence staining. The mAb 1H3 bound to three minimal linear epitopes (P62, DPPLNP; P67, DPPLNPK; P73, LKDPPLKP), which was located at Cterminus of the capsid protein of PCV2b-1A/1B, PCV2b-1C, and PCV2a-2A respectively...
February 17, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/28206909/a-highly-pathogenic-avian-derived-influenza-virus-h5n1-with-2009-pandemic-h1n1-internal-genes-demonstrates-increased-replication-and-transmission-in-pigs
#2
Eugenio J Abente, Pravina Kitikoon, Kelly M Lager, Phillip C Gauger, Tavis K Anderson, Amy L Vincent
This study investigated the pathogenicity and transmissibility of a reverse-genetics-derived highly pathogenic avian influenza (HPAI) H5N1 lineage influenza A virus that was isolated from a human, A/Iraq/755/06. We also examined surface gene reassortant viruses composed of the haemagglutinin and neuraminidase from A/Iraq/755/06 and the internal genes of a 2009 pandemic H1N1 virus, A/New York/18/2009 (2Iraq/06 : 6NY/09 H5N1), and haemagglutinin and neuraminidase from A/New York/18/2009 with the internal genes of A/Iraq/755/06 (2NY/09 : 6Iraq/06 H1N1)...
January 2017: Journal of General Virology
https://www.readbyqxmd.com/read/28206705/erysipelothrix-rhusiopathiae-bloodstream-infection-a-22-year-experience-at-mayo-clinic-minnesota
#3
E M Tan, J R Marcelin, N Adeel, R J Lewis, M J Enzler, P K Tosh
Erysipelothrix rhusiopathiae is a facultatively anaerobic Gram-positive bacillus found mostly in swine, fish and sheep. E. rhusiopathiae classically causes cutaneous eruptions in butchers, fish handlers and veterinarians. Based solely on case reports, 90% of E. rhusiopathiae bloodstream infections (BSI) have been associated with infective endocarditis (IE). To assess the true frequency of IE in E. rhusiopathiae BSI as well as other clinical associations, we performed a retrospective cohort analysis of E...
February 16, 2017: Zoonoses and Public Health
https://www.readbyqxmd.com/read/28202801/molecular-epidemiology-and-strain-comparison-between-hepatitis-e-virus-in-human-sera-and-pig-livers-during-2014-2016-in-hong-kong
#4
Martin C W Chan, Kirsty Kwok, Tin-Nok Hung, Paul K S Chan
Hepatitis E virus (HEV) causes substantial morbidity and mortality in developing countries and is considered an emerging food-borne pathogen in previously non-endemic developed countries. To investigate genetic association between human HEV infection and HEV-contaminated high-risk food in Hong Kong, we compared local virus strains obtained from hepatitis E patient sera with those surveyed from high-risk food items during 2014-2016. Twenty-four cases of laboratory-confirmed human HEV infections were identified from January 2014 to March 2016 in our hospitals...
February 15, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28202026/an-indirect-enzyme-linked-immunosorbent-assay-for-the-identification-of-antibodies-to-senecavirus-a-in-swine
#5
Cheryl M T Dvorak, Zeynep Akkutay-Yoldar, Suzanne R Stone, Steven J P Tousignant, Fabio A Vannucci, Michael P Murtaugh
BACKGROUND: Senecavirus A (SVA), a member of the family Picornaviridae, genus Senecavirus, is a recently identified single-stranded RNA virus closely related to members of the Cardiovirus genus. SVA was originally identified as a cell culture contaminant and was not associated with disease until 2007 when it was first observed in pigs with Idiopathic Vesicular Disease (IVD). Vesicular disease is sporadically observed in swine, is not debilitating, but is significant due to its resemblance to foreign animal diseases, such as foot-and-mouth disease (FMD), whose presence would be economically devastating to the United States...
February 15, 2017: BMC Veterinary Research
https://www.readbyqxmd.com/read/28198455/polymerase-cross-linking-spiral-reaction-pclsr-for-detection-of-african-swine-fever-virus-asfv-in-pigs-and-wild-boars
#6
Grzegorz Woźniakowski, Magdalena Frączyk, Andrzej Kowalczyk, Małgorzata Pomorska-Mól, Krzysztof Niemczuk, Zygmunt Pejsak
The study reports the development of a polymerase cross-linking spiral reaction (PCLSR) for the detection of African swine fever virus (ASFV) DNA in blood collected from infected pigs and wild boars. The method uses 3 specifically designed primers. Two outer-spiral primers comprising of 3' sequences complementary to ASFV p72 gene sequence and 5'end sequences complementary to exogenous gene of black widow alpha-latrotoxin as well as additional ASFV specific cross-linking primer. The method is specific exclusively to ASFV DNA without cross-reactions with cDNA of classical swine fever virus (CSFV), porcine reproductive respiratory syndrome (PRRSV) or porcine epidemic diarrhea virus (PEDV)...
February 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28197972/prevalence-and-molecular-characterization-of-the-hepatitis-e-virus-in-retail-pork-products-marketed-in-canada
#7
Oksana Mykytczuk, Jennifer Harlow, Sabah Bidawid, Nathalie Corneau, Neda Nasheri
Infection with the hepatitis E virus (HEV) is very common worldwide. HEV causes acute viral hepatitis with approximately 20 million cases per year. While HEV genotypes 1 and 2 cause large waterborne and foodborne outbreaks with a significant mortality in developing countries, genotypes 3 and 4 are more prevalent in developed countries with transmission being mostly zoonotic. In North America and Europe, HEV has been increasingly detected in swine, and exposure to pigs and pork products is considered to be the primary source of infection...
February 14, 2017: Food and Environmental Virology
https://www.readbyqxmd.com/read/28191016/incidence-and-molecular-characterization-of-hepatitis-e-virus-from-swine-in-eastern-cape-south-africa
#8
Olusesan Adeyemi Adelabu, Benson Chuks Iweriebor, U U Nwodo, Larry Chikwelu Obi, Anthony Ifeanyi Okoh
Hepatitis E virus-mediated infection is a serious public health concern in economically developing nations of the world. Globally, four major genotypes of HEV have been documented. Hepatitis E has been suggested to be zoonotic owing to the increase of evidence through various studies. Thus far, this paper reports on prevalence of hepatitis E virus among swine herd in selected communal and commercial farms in the Eastern Cape Province of South Africa. A total of 160 faecal samples were collected from swine herds in Amathole and Chris Hani District Municipalities of Eastern Cape Province for the presence of HEV...
2017: Advances in Virology
https://www.readbyqxmd.com/read/28187951/omp16-based-vaccine-encapsulated-by-alginate-chitosan-microspheres-provides-significant-protection-against-haemophilus-parasuis-in-mice
#9
Xintian Zheng, Xiaoyan Yang, Xiaohua Li, Guo-Hua Qiu, Ailing Dai, Qichun Huang, Cuiqin Huang, Xiaofeng Guo
Haemophilus parasuis (H. parasuis) is the etiological agent of swine Glässer's disease, which leads to significant economic loss in swine industry over the world. Subunit vaccine based on outer membrane protein is one of the promising choices to protect pigs against H. parasuis infection despite low immunity efficiency. In this paper, outer membrane protein 16 (Omp16) of H. parasuis encapsulated by alginate-chitosan microspheres as antigen carriers was explored for the first time in a mouse model. Our results showed that the microspheres with Omp16 induced significant higher H...
February 7, 2017: Vaccine
https://www.readbyqxmd.com/read/28183395/swine-influenza-a-h1n1-virus-siv-infection-requiring-extracorporeal-life-support-in-an-immunocompetent-adult-patient-with-indirect-exposure-to-pigs-italy-october-2016
#10
Francesca Rovida, Antonio Piralla, Federico Capra Marzani, Ana Moreno, Giulia Campanini, Francesco Mojoli, Marco Pozzi, Alessia Girello, Chiara Chiapponi, Fausto Vezzoli, Paola Prati, Elena Percivalle, Anna Pavan, Maria Gramegna, Giorgio Antonio Iotti, Fausto Baldanti
We describe a case of severe swine influenza A(H1N1) virus infection in an immunocompetent middle-aged man in October 2016 in Italy who had only indirect exposure to pigs. The patient developed a severe acute distress respiratory syndrome which was successfully supported by extracorporeal membrane oxygenation and treated with antiviral therapy. The sole risk factor for influenza was a body mass index > 30 kg/m(2). After a month of hospitalisation, the patient was discharged in good health.
February 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28182318/molecular-and-structural-changes-related-to-hepatitis-e-virus-hev-antigen-and-its-expression-in-testis-inducing-apoptosis-in-mongolian-gerbil-model
#11
Majid Hussain Soomro, Ruihan Shi, Ruiping She, Yifei Yang, Tongtong Wang, Qiaoxing Wu, Heng Li, Wenzhuo Hao
Hepatitis E virus (HEV) infection has been associated with a wide range of extrahepatic manifestations, so this study was designed to examine the effect and role of HEV on structural and molecular changes in the testicular tissues of Mongolian gerbils experimentally infected with swine HEV. HEV RNA was first detected in testis at 14 day post inoculation and reached a peak between 28 to 42 days later with viral load between 3.12 logs g-¹ to 6.23 logs g-¹ by PCR assays. Microscopic changes showed vacuolation, sloughing of germ cells, formation of multinuclear giant cells, degeneration, necrosis of tubules and damaged blood-testis barrier was observed through transmission electron microscopy...
February 9, 2017: Journal of Viral Hepatitis
https://www.readbyqxmd.com/read/28177877/antimicrobial-peptide-epinecidin-1-promotes-complete-skin-regeneration-of-methicillin-resistant-staphylococcus-aureus-infected-burn-wounds-in-a-swine-model
#12
Han-Ning Huang, Chieh-Yu Pan, Hung-Yi Wu, Jyh-Yih Chen
This report shows that the antimicrobial peptide (AMP) Epinecidin-1 (Epi-1) efficiently heals MRSA-infected heat burn injuries and provides protection from infection in a pig model. The presence of an optimal level of Epi-1 induces cell proliferation by promoting cell cycle progression through an increase in S-phase cells. Epi-1 also induces proliferation to cover the wounded region in an in vitro cell proliferation assay using immortalized human epithelial HaCaT cells. Next, the in vivo wound healing efficiency of Epi-1 was tested in heat-burned pig skin infected with MRSA under in vivo conditions...
February 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/28177368/nursery-pig-growth-performance-and-tissue-accretion-modulation-due-to-porcine-epidemic-diarrhea-virus-or-porcine-deltacoronavirus-challenge
#13
S M Curry, K A Gibson, E R Burrough, K J Schwartz, K J Yoon, N K Gabler
Porcine epidemic diarrhea virus (PEDV) and porcine deltacoronavirus (PDCoV) are both members of the family which induce clinical signs of diarrhea, dehydration, and in some circumstances, mortality. Most research has been focused on isolation, genome sequencing, pathogenicity, and virulence of these viruses, but there is little information on long-term growth performance and tissue accretion of pigs inoculated with PEDV or PDCoV. Therefore, our objective was to determine the effect of PEDV or PDCoV infection on growth performance and tissue accretion over 42 d following inoculation...
January 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28177360/genomewide-association-of-piglet-responses-to-infection-with-one-of-two-porcine-reproductive-and-respiratory-syndrome-virus-isolates
#14
E H Waide, C K Tuggle, N V L Serão, M Schroyen, A Hess, R R R Rowland, J K Lunney, G Plastow, J C M Dekkers
Porcine reproductive and respiratory syndrome (PRRS) is a devastating disease in the swine industry. Identification of host genetic factors that enable selection for improved performance during PRRS virus (PRRSV) infection would reduce the impact of this disease on animal welfare and production efficiency. We conducted genomewide association study (GWAS) analyses of data from 13 trials of approximately 200 commercial crossbred nursery-age piglets that were experimentally infected with 1 of 2 type 2 isolates of PRRSV (NVSL 97-7985 [NVSL] and KS2006-72109 [KS06])...
January 2017: Journal of Animal Science
https://www.readbyqxmd.com/read/28175983/glycyrrhizin-inhibits-porcine-epidemic-diarrhea-virus-infection-and-attenuates-the-proinflammatory-responses-by-inhibition-of-high-mobility-group-box-1-protein
#15
Chang-Chao Huan, Hua-Xia Wang, Xiang-Xiang Sheng, Rui Wang, Xin Wang, Xiang Mao
Porcine epidemic diarrhea (PED), caused by porcine epidemic diarrhea virus (PEDV) infection, leads to significant economic losses in the swine industry worldwide. In our studies, we found that glycyrrhizin, the major component of licorice root extracts, could moderately inhibit PEDV infection in Vero cells, when analyzed by western blot, qRT-PCR and a plaque formation assay. We also revealed that glycyrrhizin inhibited the entry and replication of PEDV. In addition, we demonstrated that glycyrrhizin decreased the mRNA levels of proinflammatory cytokines...
February 7, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28161213/new-perspective-of-host-micrornas-in-the-control-of-prrsv-infection
#16
REVIEW
Fang Liu, Yinping Du, Wen-Hai Feng
Porcine reproductive and respiratory syndrome (PRRS) is one of the most important diseases in pigs, leading to significant economic losses in swine industry worldwide. PRRS virus (PRRSV) is an enveloped positive single-stranded RNA virus, which mainly infects cells of monocyte/macrophage lineage. MicroRNAs (miRNAs) are small non-coding RNAs and have emerged as important regulators of virus-host cell interactions. In the past several years, scientists have been trying to understand the interaction between host miRNAs and PRRSV infection...
January 9, 2017: Veterinary Microbiology
https://www.readbyqxmd.com/read/28160432/detection-of-a-novel-sapelovirus-in-central-nervous-tissue-of-pigs-with-polioencephalomyelitis-in-the-usa
#17
P H E Arruda, B L Arruda, K J Schwartz, F Vannucci, T Resende, A Rovira, P Sundberg, J Nietfeld, B M Hause
An approximately 3,000 finishing swine operation in the United States experienced an outbreak of an atypical neurologic disease in 11-weeks-old pigs with an overall morbidity of 20% and case fatality rate of 30%. The clinical onset and progression of signs in affected pigs varied but included inappetence, compromised ambulation, ataxia, incoordination, mental dullness, paresis, paralysis and decreased response to environmental stimuli. Tissues from affected pigs were submitted for diagnostic investigation. Histopathologic examination of the cerebrum, cerebellum and spinal cord revealed severe lymphoplasmacytic and necrotizing polioencephalomyelitis with multifocal areas of gliosis and neuron satellitosis, suggestive of a neurotropic viral infection...
February 3, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28158921/the-double-antigen-elisa-concept-for-early-detection-of-e-rns-specific-classical-swine-fever-virus-antibodies-and-application-as-an-accompanying-test-for-differentiation-of-infected-from-marker-vaccinated-animals
#18
D Meyer, S Fritsche, Y Luo, C Engemann, S Blome, M Beyerbach, C-Y Chang, H-J Qiu, P Becher, A Postel
Emergency vaccination with live marker vaccines represents a promising control strategy for future classical swine fever (CSF) outbreaks, and the first live marker vaccine is available in Europe. Successful implementation is dependent on a reliable accompanying diagnostic assay that allows differentiation of infected from vaccinated animals (DIVA). As induction of a protective immune response relies on virus-neutralizing antibodies against E2 protein of CSF virus (CSFV), the most promising DIVA strategy is based on detection of E(rns) -specific antibodies in infected swine...
February 3, 2017: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/28150535/molecular-and-antimicrobial-susceptibility-characterization-of-globicatella-sulfidifaciens-isolated-from-sow-s-urinary-tract-infection
#19
Carlos E C Matajira, Luisa Z Moreno, Vasco T M Gomes, Ana Paula S Silva, Renan E Mesquita, Cristina R Amigo, Ana Paula G Christ, Maria Inês Z Sato, Andrea M Moreno
BACKGROUND: The Globicatella genus comprises Gram-positive, facultative anaerobic, α-hemolytic and catalase negative cocci morphologically and phenotypically very similar to Streptococcus and Aerococcus genus which can lead to misidentification and underestimation of this pathogen. Globicatella species have already been isolated from human and animals with heart and brain disorders. Their clinical relevance in animals, and its zoonotic potential, remains unknown due to the difficulty in their identification...
December 2017: Veterinary Quarterly
https://www.readbyqxmd.com/read/28148793/mutations-during-the-adaptation-of-h9n2-avian-influenza-virus-to-the-respiratory-epithelium-of-pigs-enhance-the-sialic-acid-binding-activity-and-the-virulence-in-mice
#20
W Yang, D Punyadarsaniya, R L O Lambertz, D C C Lee, C H Liang, D Höper, S R Leist, A Hernández-Cáceres, J Stech, M Beer, C Y Wu, C H Wong, K Schughart, F Meng, G Herrler
: The natural reservoir for influenza viruses is waterfowl from where they succeeded to cross the barrier to different mammalian species. We analyzed the adaptation of avian influenza viruses to a mammalian host by passaging an H9N2 strain three times in differentiated swine airway epithelial cells. Using precision-cut slices from the porcine lung to passage the parental virus, isolates from each of the three passages (P1-P3) were characterized by assessing growth curves and ciliostatic effects...
February 1, 2017: Journal of Virology
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