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https://www.readbyqxmd.com/read/29728336/evaluation-of-the-protective-efficacy-of-ornithodoros-moubata-midgut-membrane-antigens-selected-using-omics-and-in-silico-prediction-algorithms
#1
Prosper Obolo-Mvoulouga, Ana Oleaga, Raúl Manzano-Román, Ricardo Pérez-Sánchez
The African argasid tick Ornithodoros moubata transmits two important pathogens, the African swine fever virus and the spirochete Borrelia duttoni, the cause of human relapsing fever. To date, only conventional control measures such as widespread application of acaricides, strict control measures, and animal movement restrictions have been implemented to confine these diseases. Vaccines against tick infestations have the potential to be among the most efficacious interventions for the management of these diseases...
April 30, 2018: Ticks and Tick-borne Diseases
https://www.readbyqxmd.com/read/29713637/relevant-measures-to-prevent-the-spread-of-african-swine-fever-in-the-european-union-domestic-pig-sector
#2
REVIEW
Cristina Jurado, Marta Martínez-Avilés, Ana De La Torre, Marina Štukelj, Helena Cardoso de Carvalho Ferreira, Monica Cerioli, José Manuel Sánchez-Vizcaíno, Silvia Bellini
During the past decade, African swine fever (ASF) has spread from the Caucasus region to eastern European Union countries affecting domestic pig and wild boar populations. In order to avert ASF spread, mitigation measures targeting both populations have been established. However, despite these efforts, ASF has been reported in thirteen different countries (Georgia, Azerbaijan, Armenia, the Russian Federation, Ukraine, Belarus, Estonia, Latvia, Lithuania, Poland, Moldova, Czech Republic, and Romania). In the absence of an effective vaccine or treatment to ASF, introduction and spread of ASF onto domestic pig farms can only be prevented by strict compliance to control measures...
2018: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/29695831/deletion-at-the-5-end-of-estonian-asfv-strains-associated-with-an-attenuated-phenotype
#3
Laura Zani, Jan Hendrik Forth, Leonie Forth, Imbi Nurmoja, Simone Leidenberger, Julia Henke, Jolene Carlson, Christiane Breidenstein, Arvo Viltrop, Dirk Höper, Carola Sauter-Louis, Martin Beer, Sandra Blome
African swine fever (ASF) was introduced into the Eastern European Union in 2014 and led to considerable mortality among wild boar. In contrast, unexpected high antibody prevalence was reported in hunted wild boar in north-eastern Estonia. One of the causative virus strains was recently characterized. While it still showed rather high virulence in the majority of experimentally infected animals, one animal survived and recovered completely. Here, we report on the follow-up characterization of the isolate obtained from the survivor in the acute phase of infection...
April 25, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29679458/no-evidence-for-long-term-carrier-status-of-pigs-after-african-swine-fever-virus-infection
#4
A Petrov, J H Forth, L Zani, M Beer, S Blome
This study targeted the assessment of a potential African swine fever virus (ASFV) carrier state of 30 pigs in total which were allowed to recover from infection with ASFV "Netherlands'86" prior exposure to six healthy sentinel pigs for more than 2 months. Throughout the whole trial, blood and swab samples were subjected to routine virological and serological investigations. At the end of the trial, necropsy of all animals was performed and viral persistence and distribution were assessed. Upon infection, a wide range of clinical and pathomorphological signs were observed...
April 20, 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29674053/chimeric-dna-lna-based-biosensor-for-the-rapid-detection-of-african-swine-fever-virus
#5
Massimo Biagetti, Massimiliano Cuccioloni, Laura Bonfili, Valentina Cecarini, Carla Sebastiani, Ludovica Curcio, Monica Giammarioli, Gian Mario De Mia, Anna Maria Eleuteri, Mauro Angeletti
African swine fever (ASF) virus is a DNA virus responsible for a severe haemorrhagic fever in pigs, which (still in the absence of vaccination strategies) results in high mortality rates. Herein, we present a biosensor-based method for the detection of ASF viral DNA in the blood of pigs. The biosensor exploits a single-strand DNA probe with locked nucleic acid nucleotides (LNA) substitutions as the complementary recognition element for the conserved region of vp72 gene of ASF virus. The biosensor was calibrated using qPCR-quantified ASF viral DNA extracted from the blood of pigs experimentally infected with the virulent Italian isolate 49/08, genotype I...
July 1, 2018: Talanta
https://www.readbyqxmd.com/read/29627336/real-time-analysis-of-the-cytopathic-effect-of-african-swine-fever-virus
#6
Galina Burmakina, Kirill Bliznetsov, Alexander Malogolovkin
Conventional methods, which quantitatively assess virus replication, are based on direct examination of viral cytopathic effect (CPE), which is time consuming, tedious and based on endpoint reading. The Real-Time Cell Analysis (RTCA) xCELLigence® system offers an alternative approach to evaluate virus-induced CPE, and here was evaluated as a means to dynamically assess CPE caused by African swine fever virus (ASFV). RTCA was used to identify optimum time for ASFV infection based on cell index (CI) and to evaluate ASFV CPE kinetics in COS-1 cells...
April 5, 2018: Journal of Virological Methods
https://www.readbyqxmd.com/read/29616228/modeling-the-effects-of-duration-and-size-of-the-control-zones-on-the-consequences-of-a-hypothetical-african-swine-fever-epidemic-in-denmark
#7
Tariq Halasa, Anette Bøtner, Sten Mortensen, Hanne Christensen, Sisse Birk Wulff, Anette Boklund
African swine fever (ASF) is a notifiable infectious disease. The disease is endemic in certain regions in Eastern Europe constituting a risk of ASF spread toward Western Europe. Therefore, as part of contingency planning, it is important to continuously explore strategies that can effectively control an epidemic of ASF. A previously published and well documented simulation model for ASF virus spread between herds was used to examine the epidemiologic and economic impacts of the duration and size of the control zones around affected herds...
2018: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/29609966/african-swine-fever-virus-asfv-protection-mediated-by-nh-p68-and-nh-p68-recombinant-live-attenuated-viruses
#8
Carmina Gallardo, Elena G Sánchez, Daniel Pérez-Núñez, Marisa Nogal, Patricia de León, Ángel L Carrascosa, Raquel Nieto, Alejandro Soler, María Luisa Arias, Yolanda Revilla
The risk of spread of African swine fever virus (ASFV) from Russia and Caucasian areas to several EU countries has recently emerged, making it imperative to improve our knowledge and defensive tools against this important pathogen. The ASFV genome encodes many genes which are not essential for virus replication but are known to control host immune evasion, such as NFκB and the NFAT regulator A238L, the apoptosis inhibitor A224L, the MHC-I antigen presenting modulator EP153R, and the A276R gene, involved in modulating type I IFN...
March 30, 2018: Vaccine
https://www.readbyqxmd.com/read/29605728/the-l83l-orf-of-african-swine-fever-virus-strain-georgia-encodes-for-a-non-essential-gene-that-interacts-with-the-host-protein-il-1%C3%AE
#9
Manuel V Borca, Vivian O'Donnell, Lauren G Holinka, Elizabeth Ramírez-Medina, Benjamin A Clark, Elizabeth A Vuono, Keith Berggren, Marialexia Alfano, Lucas B Carey, Juergen A Richt, Guillermo R Risatti, Douglas P Gladue
African swine fever virus (ASFV) causes a contagious and frequently lethal disease of pigs causing significant economic consequences to the swine industry. The ASFV genome encodes for more than 150 genes, but only a few of them have been studied in detail. Here we report the characterization of open reading frame L83L which encodes a highly conserved protein across all ASFV isolates. A recombinant ASFV harboring a HA tagged L83L protein was developed (ASFV-G-L83L-HA) and used to demonstrate that L83L is a transiently expressed early virus protein...
March 29, 2018: Virus Research
https://www.readbyqxmd.com/read/29565243/ictv-virus-taxonomy-profile-asfarviridae
#10
Covadonga Alonso, Manuel Borca, Linda Dixon, Yolanda Revilla, Fernando Rodriguez, Jose M Escribano, Ictv Report Consortium
The family Asfarviridae includes the single species African swine fever virus, isolates of which have linear dsDNA genomes of 170-194 kbp. Virions have an internal core, an internal lipid membrane, an icosahedral capsid and an outer lipid envelope. Infection of domestic pigs and wild boar results in an acute haemorrhagic fever with transmission by contact or ingestion, or by ticks of the genus Ornithodoros. Indigenous pigs act as reservoirs in Africa, where infection is endemic, and from where introductions occur periodically to Europe...
March 22, 2018: Journal of General Virology
https://www.readbyqxmd.com/read/29559099/evaluation-of-biological-and-socio-economic-factors-related-to-persistence-of-african-swine-fever-in-sardinia
#11
Stefano Cappai, Sandro Rolesu, Annamaria Coccollone, Alberto Laddomada, Federica Loi
Despite the implementation of several eradication programmes, African swine fever (ASF), a viral disease in pigs caused by a DNA virus (ASFV), has been present in Sardinia (Italy) since 1978. Several studies have been carried out on the epidemiology of ASF in Sardinia, aimed at attaining a better understanding of the role of the risk factors related to ASFV persistence, but those studies did not address the social aspects involved. This work sought to bridge this gap, identifying the main social risk factors associated with ASF persistence...
April 1, 2018: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/29558524/survival-of-viral-pathogens-in-animal-feed-ingredients-under-transboundary-shipping-models
#12
Scott A Dee, Fernando V Bauermann, Megan C Niederwerder, Aaron Singrey, Travis Clement, Marcelo de Lima, Craig Long, Gilbert Patterson, Maureen A Sheahan, Ana M M Stoian, Vlad Petrovan, Cassandra K Jones, Jon De Jong, Ju Ji, Gordon D Spronk, Luke Minion, Jane Christopher-Hennings, Jeff J Zimmerman, Raymond R R Rowland, Eric Nelson, Paul Sundberg, Diego G Diel
The goal of this study was to evaluate survival of important viral pathogens of livestock in animal feed ingredients imported daily into the United States under simulated transboundary conditions. Eleven viruses were selected based on global significance and impact to the livestock industry, including Foot and Mouth Disease Virus (FMDV), Classical Swine Fever Virus (CSFV), African Swine Fever Virus (ASFV), Influenza A Virus of Swine (IAV-S), Pseudorabies virus (PRV), Nipah Virus (NiV), Porcine Reproductive and Respiratory Syndrome Virus (PRRSV), Swine Vesicular Disease Virus (SVDV), Vesicular Stomatitis Virus (VSV), Porcine Circovirus Type 2 (PCV2) and Vesicular Exanthema of Swine Virus (VESV)...
2018: PloS One
https://www.readbyqxmd.com/read/29553337/identification-of-wild-boar-habitat-epidemiologic-cycle-in-african-swine-fever-epizootic
#13
Erika Chenais, Karl Ståhl, Vittorio Guberti, Klaus Depner
The African swine fever epizootic in central and eastern European Union member states has a newly identified component involving virus transmission by wild boar and virus survival in the environment. Insights led to an update of the 3 accepted African swine fever transmission models to include a fourth cycle: wild boar-habitat.
April 2018: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29553323/african-swine-fever-virus-siberia-russia-2017
#14
Denis Kolbasov, Ilya Titov, Sodnom Tsybanov, Andrey Gogin, Alexander Malogolovkin
African swine fever (ASF) is arguably the most dangerous and emerging swine disease worldwide. ASF is a serious problem for the swine industry. The first case of ASF in Russia was reported in 2007. We report an outbreak of ASF in Siberia, Russia, in 2017.
April 2018: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/29552564/consumption-of-big-game-remains-by-scavengers-a-potential-risk-as-regards-disease-transmission-in-central-spain
#15
Ricardo Carrasco-Garcia, Patricia Barroso, Javier Perez-Olivares, Vidal Montoro, Joaquín Vicente
Understanding the role that facultative scavenger species may play in spreading infectious pathogens, and even becoming reservoirs for humans, domestic and wild ungulates or, on the contrary, preventing the spread of disease, requires a prior understanding of the pattern of carrion scavenging in specific scenarios. The objectives of this paper are (i) to describe the guild of vertebrate scavengers and (ii) to study the species-specific, habitat, and management-related factors involved in the usage of gut piles in South Central Spain (SCS), a tuberculosis (TB) endemic area...
2018: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/29551143/african-swine-fever-virus-biology-and-vaccine-approaches
#16
Yolanda Revilla, Daniel Pérez-Núñez, Juergen A Richt
African swine fever (ASF) is an acute and often fatal disease affecting domestic pigs and wild boar, with severe economic consequences for affected countries. ASF is endemic in sub-Saharan Africa and the island of Sardinia, Italy. Since 2007, the virus emerged in the republic of Georgia, and since then spread throughout the Caucasus region and Russia. Outbreaks have also been reported in Belarus, Ukraine, Lithuania, Latvia, Estonia, Romania, Moldova, Czech Republic, and Poland, threatening neighboring West European countries...
2018: Advances in Virus Research
https://www.readbyqxmd.com/read/29536638/management-of-hunting-waste-as-control-measure-for-tuberculosis-in-wild-ungulates-in-south-central-spain
#17
D Cano-Terriza, M A Risalde, S Jiménez-Ruiz, J Vicente, J Isla, J Paniagua, I Moreno, C Gortázar, J A Infantes-Lorenzo, I García-Bocanegra
In recent decades, habitat change and the intensive management of wild ungulates for hunting have led to an increase in their populations in south-central Spain. This implies a higher generation of hunting waste, which can favour the transmission of infectious diseases, including tuberculosis (TB). The aim of this study was to assess the usefulness of the proper disposal of hunting waste as TB control measure in wild boar (Sus scrofa) and red deer (Cervus elaphus) during the 2008/2009 to 2016/2017 hunting seasons...
March 13, 2018: Transboundary and Emerging Diseases
https://www.readbyqxmd.com/read/29519515/interaction-of-porcine-monocyte-derived-dendritic-cells-with-african-swine-fever-viruses-of-diverse-virulence
#18
Giulia Franzoni, Simon P Graham, Giovanna Sanna, Pierpaolo Angioi, Mariangela S Fiori, Antonio Anfossi, Massimo Amadori, Silvia Dei Giudici, Annalisa Oggiano
African swine fever (ASF) is a devastating disease for which there is no vaccine. The ASF virus (ASFV) can infect dendritic cell (DC), but despite the critical role these cells play in induction of adaptive immunity, few studies investigated their response to ASFV infection. We characterized the in vitro interactions of porcine monocyte-derived DCs (moDC) with a virulent (22653/14), a low virulent (NH/P68) and an avirulent (BA71V) ASFV strain. At a high multiplicity of infection (MOI = 1), all three strains infected immature moDC...
March 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29519508/modulation-of-type-i-interferon-signaling-by-african-swine-fever-virus-asfv-of-different-virulence-l60-and-nhv-in-macrophage-host-cells
#19
Raquel Portugal, Alexandre Leitão, Carlos Martins
ASFV causes an important disease of domestic swine and wild boar. Currently no vaccine is available, highlighting the necessity to understand ASFV modulation of innate immune responses in natural host cells. With this aim, macrophage cultures enriched in SWC9 and CD163 differentiation markers were infected in parallel with high virulent ASFV/L60 and low virulent ASFV/NHV, the latter lacking MGF 360 and 505/530 genes associated with type I interferon (IFN I) control. IFN I production and signaling were studied after completion of the viral cycles...
March 2018: Veterinary Microbiology
https://www.readbyqxmd.com/read/29496103/african-swine-fever-control-and-market-integration-in-ugandan-peri-urban-smallholder-pig-value-chains-an-ex-ante-impact-assessment-of-interventions-and-their-interaction
#20
Emily Ouma, Michel Dione, Rosemirta Birungi, Peter Lule, Lawrence Mayega, Kanar Dizyee
Pig production in peri-urban smallholder value chains in Uganda is severely constrained by impact of disease, particularly African swine fever (ASF), and the economic consequences of an inefficient pig value chain. Interventions in the form of biosecurity to control ASF disease outbreaks and pig business hub models to better link smallholder farmers to pig markets have the potential to address the constraints. However, there is a dearth of evidence of the effects of the interventions on performance and distribution of outcomes along the pig value chain...
March 1, 2018: Preventive Veterinary Medicine
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