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Biosensors

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https://www.readbyqxmd.com/read/28335390/a-simple-metallothionein-based-biosensor-for-enhanced-detection-of-arsenic-and-mercury
#1
Gordon W Irvine, Swee Ngin Tan, Martin J Stillman
Metallothioneins (MTs) are a family of cysteine-rich proteins whose biological roles include the regulation of essential metal ions and protection against the harmful effects of toxic metals. Due to its high affinity for many toxic, soft metals, recombinant human MT isoform 1a was incorporated into an electrochemical-based biosensor for the detection of As(3+) and Hg(2+). A simple design was chosen to maximize its potential in environmental monitoring and MT was physically adsorbed onto paper discs placed on screen-printed carbon electrodes (SPCEs)...
March 13, 2017: Biosensors
https://www.readbyqxmd.com/read/28335352/nano-biosensor-for-monitoring-the-neural-differentiation-of-stem-cells
#2
REVIEW
Jin-Ho Lee, Taek Lee, Jeong-Woo Choi
In tissue engineering and regenerative medicine, monitoring the status of stem cell differentiation is crucial to verify therapeutic efficacy and optimize treatment procedures. However, traditional methods, such as cell staining and sorting, are labor-intensive and may damage the cells. Therefore, the development of noninvasive methods to monitor the differentiation status in situ is highly desirable and can be of great benefit to stem cell-based therapies. Toward this end, nanotechnology has been applied to develop highly-sensitive biosensors to noninvasively monitor the neural differentiation of stem cells...
November 28, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28335348/electrochemical-synthesis-of-polypyrrole-reduced-graphene-oxide-and-gold-nanoparticles-composite-and-its-application-to-hydrogen-peroxide-biosensor
#3
Baoyan Wu, Na Zhao, Shihua Hou, Cong Zhang
Here we report a facile eco-friendly one-step electrochemical approach for the fabrication of a polypyrrole (PPy), reduced graphene oxide (RGO), and gold nanoparticles (nanoAu) biocomposite on a glassy carbon electrode (GCE). The electrochemical behaviors of PPy-RGO-nanoAu and its application to electrochemical detection of hydrogen peroxide were investigated by cyclic voltammetry. Graphene oxide and pyrrole monomer were first mixed and casted on the surface of a cleaned GCE. After an electrochemical processing consisting of the electrooxidation of pyrrole monomer and simultaneous electroreduction of graphene oxide and auric ions (Au(3+)) in aqueous solution, a PPy-RGO-nanoAu biocomposite was synthesized on GCE...
November 21, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28335287/non-enzymatic-glucose-biosensor-based-on-cuo-decorated-ceo%C3%A2-nanoparticles
#4
Panpan Guan, Yongjian Li, Jie Zhang, Wei Li
Copper oxide (CuO)-decorated cerium oxide (CeO₂) nanoparticles were synthesized and used to detect glucose non-enzymatically. The morphological characteristics and structure of the nanoparticles were characterized through transmission electron microscopy, X-ray photoelectron spectroscopy, and X-ray diffraction. The sensor responses of electrodes to glucose were investigated via an electrochemical method. The CuO/CeO₂ nanocomposite exhibited a reasonably good sensitivity of 2.77 μA mM(-1)cm(-2), an estimated detection limit of 10 μA, and a good anti-interference ability...
August 26, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28335236/complex-morphology-metal-based-nanostructures-fabrication-characterization-and-applications
#5
REVIEW
Antonella Gentile, Francesco Ruffino, Maria Grazia Grimaldi
Due to their peculiar qualities, metal-based nanostructures have been extensively used in applications such as catalysis, electronics, photography, and information storage, among others. New applications for metals in areas such as photonics, sensing, imaging, and medicine are also being developed. Significantly, most of these applications require the use of metals in the form of nanostructures with specific controlled properties. The properties of nanoscale metals are determined by a set of physical parameters that include size, shape, composition, and structure...
June 6, 2016: Nanomaterials
https://www.readbyqxmd.com/read/28334627/a-novel-versatile-microbiosensor-for-local-hydrogen-detection-by-means-of-scanning-photoelectrochemical-microscopy
#6
Fangyuan Zhao, Felipe Conzuelo, Volker Hartmann, Huaiguang Li, Stefanie Stapf, Marc M Nowaczyk, Matthias Rögner, Nicolas Plumeré, Wolfgang Lubitz, Wolfgang Schuhmann
The development of a versatile microbiosensor for hydrogen detection is reported. Carbon-based microelectrodes were modified with a [NiFe]-hydrogenase embedded in a viologen-modified redox hydrogel for the fabrication of a sensitive hydrogen biosensor By integrating the microbiosensor in a scanning photoelectrochemical microscope, it was capable of serving simultaneously as local light source to initiate photo(bio)electrochemical reactions while acting as sensitive biosensor for the detection of hydrogen. A hydrogen evolution biocatalyst based on photosystem 1-platinum nanoparticle biocomplexes embedded into a specifically designed redox polymer was used as a model for proving the capability of the developed hydrogen biosensor for the detection of hydrogen upon localized illumination...
March 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28334626/label-free-electrochemiluminescent-biosensor-for-rapid-and-sensitive-detection-of-pseudomonas-aeruginosa-using-phage-as-highly-specific-recognition-agent
#7
Huan Yue, Yong He, Enci Fan, Lin Wang, Shuguang Lu, Zhifeng Fu
A virulent phage named as PaP1 was isolated from hospital sewage based on a lambda phage isolation protocol. This phage showed a strong and highly specific binding ability to Pseudomonas aeruginosa (P. aeruginosa). Using this isolated phage as a recognition agent, a novel electrochemiluminescent (ECL) biosensor was developed for label-free detection of P. aeruginosa. The biosensor was fabricated through depositing phage-conjugated carboxyl graphene onto the surface of a glass carbon electrode. After specific binding of the host bacteria through the adsorption of P...
March 18, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/28333943/fabrication-of-sensitive-enzymatic-biosensor-based-on-multi-layered-reduced-graphene-oxide-added-ptau-nanoparticles-modified-hybrid-electrode
#8
Md Faruk Hossain, Jae Y Park
A highly sensitive amperometric glucose sensor was developed by immobilization of glucose oxidase (GOx) onto multi-layer reduced graphene oxide (MRGO) sheets decorated with platinum and gold flower-like nanoparticles (PtAuNPs) modified Au substrate electrode. The fabricated MRGO/PtAuNPs modified hybrid electrode demonstrated high electrocatalytic activities toward oxidation of H2O2, to which it had a wide linear response that ranged from 0.5 to 8 mM (R2 = 0.997), and high sensitivity of 506.25 μA/mMcm2. Furthermore, glucose oxidase-chitosan composite and cationic polydiallyldimethylammonium chloride (PDDA) were assembled by a casting method on the surface of MRGO/PtAuNPs modified electrode...
2017: PloS One
https://www.readbyqxmd.com/read/28333269/twin-layer-biosensor-for-real-time-monitoring-of-alkane-metabolism
#9
Tapio Lehtinen, Ville Santala, Suvi Santala
Intracellular metabolic sensors can be used for efficient screening and optimization of microbial cell factories. In particular, the sensors are useful in acquiring information about pathway dynamics and bottlenecks in a straightforward manner. Here, we developed a twin-layer biosensor which functions simultaneously at two levels: through transcription factor mediated sensing and enzyme-metabolite interaction, providing insights into the dynamics of alkane metabolism. In addition, the sensor can be used for monitoring either alkane degradation or biosynthesis, depending on the used cellular context...
March 15, 2017: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/28331314/gold-nanoparticles-based-electrochemical-method-for-the-detection-of-protein-kinase-with-a-peptide-like-inhibitor-as-the-bioreceptor
#10
Kai Sun, Yong Chang, Binbin Zhou, Xiaojin Wang, Lin Liu
This article presents a general method for the detection of protein kinase with a peptide-like kinase inhibitor as the bioreceptor, and it was done by converting gold nanoparticles (AuNPs)-based colorimetric assay into sensitive electrochemical analysis. In the colorimetric assay, the kinase-specific aptameric peptide triggered the aggregation of AuNPs in solution. However, the specific binding of peptide to the target protein (kinase) inhibited its ability to trigger the assembly of AuNPs. In the electrochemical analysis, peptides immobilized on a gold electrode and presented as solution triggered together the in situ formation of AuNPs-based network architecture on the electrode surface...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28330085/bioprospecting-and-biotechnological-applications-of-fungal-laccase
#11
REVIEW
Pooja Upadhyay, Rahul Shrivastava, Pavan Kumar Agrawal
Laccase belongs to a small group of enzymes called the blue multicopper oxidases, having the potential ability of oxidation. It belongs to enzymes, which have innate properties of reactive radical production, but its utilization in many fields has been ignored because of its unavailability in the commercial field. There are diverse sources of laccase producing organisms like bacteria, fungi and plants. In fungi, laccase is present in Ascomycetes, Deuteromycetes, Basidiomycetes and is particularly abundant in many white-rot fungi that degrade lignin...
June 2016: 3 Biotech
https://www.readbyqxmd.com/read/28327470/synthesis-of-novel-nitrogen-doped-carbon-dots-for-highly-selective-detection-of-iron-ion
#12
Pengfei Lv, Yixin Yao, Huimin Zhou, Jin Zhang, Zengyuan Pang, Kelong Ao, Yibing Cai, Qufu Wei
Herein, we report an eco-friendly and simple fluorescent nitrogen-doped carbon quantum dot (N-CQD) biosensor which was synthesized via a hydrothermal method using erhanediamine (EDA) and citric acid (CA) as precursors. The surface functionalization of N-CQDs exhibited a bright blue emission under the excitation wavelength of 350 nm. The obtained N-CQDs were characterized by atomic force microscopy (AFM), Fourier transform infrared spectroscopy, x-ray photoelectron spectroscopy, and transmission electron microscopy...
March 22, 2017: Nanotechnology
https://www.readbyqxmd.com/read/28326779/illuminating-photochemistry-of-an-excitation-ratiometric-fluorescent-protein-calcium-biosensor
#13
Longteng Tang, Yanli Wang, Weimin Liu, Yongxin Zhao, Robert E Campbell, Chong Fang
Fluorescent protein (FP) based biosensors have become an important and promising tool to track metal ion movement inside living systems. Their working principles after light irradiation, however, remain elusive. To facilitate the rational design of biosensors, we dissect the fluorescence modulation mechanism of a newly developed excitation ratiometric green FP-based Ca(2+) biosensor, GEX-GECO1, using femtosecond stimulated Raman spectroscopy (FSRS) in the electronic excited state. Upon 400 nm photoexcitation, characteristic vibrational marker bands at ~1180 and 1300 cm(-1) show concomitant decay and rise dynamics, probing the progression of an ultrafast excited state proton transfer (ESPT) reaction...
March 22, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28326747/laboratory-methods-for-diagnostics-of-hiv-infection-and-hiv-associated-neuroinfections
#14
(no author information available yet)
HIV Infection resulting in AIDS remains serious global public health problem. In the fight with the global health problem plays a key role a simple, reliable and fast diagnostics. An important method in diagnostics is the identification and detection of viral capside p24 antigen levels. Fourth generation tests for the diagnostics of HIV infection simultaneously detect the presence of HIV antibodies and p24 antigen. Based on the monitoring of CD4 count, we can estimate the stage in which the infection is, and we can also suggest a therapeutic approach...
January 22, 2017: Neuro Endocrinology Letters
https://www.readbyqxmd.com/read/28326608/dnazyme-aptamer-or-aptamer-dnazyme-paradigm-biochemical-approach-for-aflatoxin-analysis
#15
Marzieh Jafari, Mohsen Rezaei, Heibatullah Kalantari, Maryam Tabarzad, Bahram Daraei
DNAzyme and aptamer conjugations have already been used for sensitive and accurate detection of several molecules. In this study, we tested the relationship between conjugation orientation of DNAzyme and Aflatoxin B1 aptamer and their subsequent peroxidase activity. Circular dichroism (CD) spectroscopy and biochemical analysis were used here to difference between these two conjugation patterns. Results showed that DNAzyme-aptamer has more catalytic activity and efficiency than aptamer-DNAzyme. Thereby, DNAzyme-aptamer with its superior efficiency can be used for design and development of more sensitive Aflatoxin B1 DNA based biosensors...
March 22, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28326226/comparison-of-four-methods-for-the-biofunctionalization-of-gold-nanorods-by-the-introduction-of-sulfhydryl-groups-to-antibodies
#16
Xuefeng Wang, Zhong Mei, Yanyan Wang, Liang Tang
Introducing sulfhydryl groups to biomolecules to functionalize gold nanorods (GNRs) is an attractive method that involves the creation of a strong Au-S bond. Previously, we developed a facile method to functionalize GNR surfaces by thiolating antibodies using Traut's reagent. In the current study, we evaluated several methods for the introduction of thiol groups onto the surface of GNRs by using Traut's reagent, dithiotreitol (DTT), dithiolaromatic PEG6-CONHNH2, and thiol-polyethylene glycolamine (SH-PEG-NH2) combined with EDC reaction...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28326089/dynamic-regulation-of-auxin-response-during-rice-development-revealed-by-newly-established-hormone-biosensor-markers
#17
Jing Yang, Zheng Yuan, Qingcai Meng, Guoqiang Huang, Christophe Périn, Charlotte Bureau, Anne-Cécile Meunier, Mathieu Ingouff, Malcolm J Bennett, Wanqi Liang, Dabing Zhang
The hormone auxin is critical for many plant developmental processes. Unlike the model eudicot plant Arabidopsis (Arabidopsis thaliana), auxin distribution and signaling in rice tissues has not been systematically investigated due to the absence of suitable auxin response reporters. In this study we observed the conservation of auxin signaling components between Arabidopsis and model monocot crop rice (Oryza sativa), and generated complementary types of auxin biosensor constructs, one derived from the Aux/IAA-based biosensor DII-VENUS but constitutively driven by maize ubiquitin-1 promoter, and the other termed DR5-VENUS in which a synthetic auxin-responsive promoter (DR5rev ) was used to drive expression of the yellow fluorescent protein (YFP)...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28325051/bioavailability-of-immobilized-epidermal-growth-factor-covalent-versus-noncovalent-grafting
#18
Nesrine Riahi, Frederic Murschel, Sophie Lerouge, Yves Durocher, Olivier Henry, Gregory De Crescenzo
In an effort to rationalize and optimize an antiapoptotic coating combining chondroitin sulfate (CS) and epidermal growth factor (EGF) for vascular applications, the authors here report the comparison of two grafting strategies aiming to display EGF in an oriented fashion on CS. For that purpose, the authors produced, purified, and characterized a chimeric protein corresponding to EGF that was N-terminally fused to a cysteine and a coil peptide. The chimera was covalently immobilized via its free thiol group or captured via coiled-coil interactions at the surface of a biosensor or on a chondroitin sulfate coating in multiwell plates, mimicking the coating that was previously developed by them for stent-graft surfaces...
March 21, 2017: Biointerphases
https://www.readbyqxmd.com/read/28325003/nanostructured-bioluminescence-quenching-sensor-for-detecting-glucose
#19
Longyan Chen, Longyi Chen, Jin Zhang
A new nanostructured biosensor by applying bioluminescent quenching technique is developed for detecting small molecules, e.g. glucose, at low concentration. It demonstrates the effect of glucose on the interaction between β-cyclodextrin (β-CD) conjugated luciferase and phenylboronic acid (PBA) conjugated gold nanoparticles (Au NPs).
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28324860/direct-detection-of-two-different-tumor-derived-extracellular-vesicles-by-sam-aunis-lspr-biosensor
#20
Abhimanyu Thakur, Guangyu Qiu, Siu-Pang Ng, Jintao Guan, Jianbo Yue, Youngjin Lee, Chi-Man Lawrence Wu
Extracellular vesicles (EVs) are abundant in various biological fluids including blood, saliva, urine, as well as extracellular milieu. Accumulating evidence has indicated that EVs, which contain functional proteins and small RNAs, facilitate intercellular communication between neighbouring cells, and are critical to maintain various physiological processes. In contrast, EV-derived toxic signals can spread out over the tissues adjacent to the injured area in certain diseases, including brain tumors and neurodegenerative disorders...
March 18, 2017: Biosensors & Bioelectronics
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