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supplementary phosphatidylserine

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https://www.readbyqxmd.com/read/21028921/simple-model-of-the-transduction-of-cell-penetrating-peptides
#1
K Cahill
Cell-penetrating peptides (CPPs) such as HIV's trans-activating transcriptional activator (TAT) and polyarginine rapidly pass through the plasma membranes of mammalian cells by an unknown mechanism called transduction. They may be medically useful when fused to well-chosen chains of fewer than about 35 amino acids. The author offers a simple model of transduction in which phosphatidylserines and CPPs effectively form two plates of a capacitor with a voltage sufficient to cause the formation of transient pores (electroporation)...
September 2009: IET Systems Biology
https://www.readbyqxmd.com/read/15256910/enrichment-of-non-apoptotic-human-spermatozoa-after-cryopreservation-by-immunomagnetic-cell-sorting
#2
S Grunewald, U Paasch, H J Glander
Cryopreservation increases the rate of apoptotic spermatozoa with decreased capability to fertilise oocytes. In order to optimise the fertilisation rates, especially in assisted reproduction the use of apoptotic sperms should be avoided. Early events of apoptosis in cryopreserved spermatozoa are not detectable by conventional methods. However, the surface of apoptotic spermatozoa is characterised by externalisation of phosphatidylserine (PS), which has a high affinity to Annexin V. Therefore, colloid paramagnetic Annexin-V-conjugated microbeads (AN-MB) were tested for their ability to eliminate apoptotic spermatozoa from a total of 40 fresh and in TEST yolk buffer cryopreserved semen samples which were provided by 15 healthy volunteers...
2001: Cell and Tissue Banking
https://www.readbyqxmd.com/read/8054258/effect-of-dietary-magnesium-deficiency-with-without-cholesterol-supplementation-on-phospholipid-content-in-liver-plasma-and-erythrocytes-of-rabbits
#3
Q Zhou, M M Mahfouz, F A Kummerow
The effect of magnesium deficiency with/without supplementary cholesterol on phospholipid content in liver, plasma and erythrocytes was investigated in New Zealand White male rabbits. After the rabbits had been fed a low magnesium and/or high cholesterol diet for seven weeks, the phospholipid content in liver, plasma and erythrocytes was measured. Dietary magnesium deficiency produced a marked elevation of total phospholipid and certain individual phospholipids in the rabbit liver. The elevation was accentuated by cholesterol supplementation...
March 1994: Magnesium Research: Official Organ of the International Society for the Development of Research on Magnesium
https://www.readbyqxmd.com/read/4060575/reversible-inactivation-and-reactivation-of-vaccinia-virus-by-manipulation-of-viral-lipid-composition
#4
M Oie
The role of phospholipids in vaccinia virus was investigated by substituting viral lipids with specific phospholipids. Treatment of virus with sodium dodecyl sulfate, sodium deoxycholate, or Nonidet-P40 (NP-40) resulted in almost complete removal of viral lipid and led to inactivation of the virus. The inactivation induced by the former two was irreversible, but NP-40-treated virus was reactivated upon reassociation with phospholipids. Individual phospholipids, including phosphatidylserine (PS), phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, lysolecithin, sphingomyelin, and acyl bis(monoacylglycero)phosphate (ABMP), were tested for ability to reactivate NP-40-treated virus...
April 30, 1985: Virology
https://www.readbyqxmd.com/read/2954026/effect-of-fish-oil-supplementation-on-the-n-3-fatty-acid-content-of-red-blood-cell-membranes-in-preterm-infants
#5
S E Carlson, P G Rhodes, V S Rao, D E Goldgar
Very low birth weight infants demonstrate significant reductions in red blood cell membrane docosahexaenoic acid (DHA, 22:6n-3) following delivery unless fed human milk. The purpose of the present study was to determine if a dietary source of DHA (MaxEPA, R. P. Scherer Corporation, Troy, MI) could prevent the decline in red blood cell phospholipid DHA in very low birth weight infants whose enteral feeding consisted of a preterm formula without DHA. Longitudinal data were obtained on membrane phospholipid DHA in both unsupplemented and MaxEPA-supplemented infants by a combination of thin-layer and gas chromatography...
May 1987: Pediatric Research
https://www.readbyqxmd.com/read/2838083/fluoride-dependent-calcium-induced-platelet-procoagulant-activity-shows-that-calpain-is-involved-in-increased-phospholipid-transbilayer-movement
#6
P F Verhallen, E M Bevers, P Comfurius, R F Zwaal
Treatment of platelets with fluoride (10 mM) was found to result in a transient increase in Ca2+-permeability of the platelet plasma membrane. This phenomenon was used to provide supplementary evidence for the suggestions made earlier (Comfurius et al. (1985) Biochim. Biophys. Acta 815, 143; Verhallen et al. (1987) Biochim. Biophys. Acta 903, 206), that cytoskeletal disrupture by calpain is involved in the process leading to transbilayer movement of phosphatidylserine during expression of platelet procoagulant activity...
July 7, 1988: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/2773445/-the-significance-of-studying-phospholipids-in-the-diagnosis-of-hypothyroidism
#7
N B Zelinskaia
Phospholipids and their fractions were examined in 85 patients with hypothyroidism of various severity grades in the state of decompensation. It was established that hypothyroidism is characterized by essential disorders of the phospholipid metabolism which occurs already at the initial stages of the disease advances with progression of its severity and is manifested in an increase of phosphatidylserine, lysophosphatidylcholine, sphingomyelin, phosphatidylethanolamine and reduction of phosphatidylcholine. Examination of phospholipids and their fractions is one of the important supplementary objective methods of diagnosis of hypothyroidism, especially, in young persons, a criterion of metabolic decompensation of the disease...
May 1989: Vrachebnoe Delo
https://www.readbyqxmd.com/read/219891/divalent-cation-binding-to-phospholipid-veiscles-dependence-on-temperature-and-lipid-fluidity
#8
J S Puskin, T Martin
Mn2+ binding to vesicles prepared from several different species of anionic phospholipids was determined as a function of temperature by electron paramagnetic resonance (EPR). The Mn2+ affinities of phosphatidylserine, cardiolipin and egg yolk phosphatidylglycerol all increased monitonically with temperature. Vesicles prepared from hydrogenated and natural (bovine) phosphatidylserine were monitored with respect to hydrocarbon chain fluidity as well as Mn2+ binding. Contrary to expectations based on surface potential considerations, the affinity of phosphatidylserine for divalent cations was apparently not lowered in going from the gel state to the lipid crystalline state of the bilayer...
March 23, 1979: Biochimica et Biophysica Acta
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