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https://www.readbyqxmd.com/read/29713468/dietary-resveratrol-improves-antioxidant-status-of-sows-and-piglets-and-regulates-antioxidant-gene-expression-in-placenta-by-keap1-nrf2-pathway-and-sirt1
#1
Qingwei Meng, Tao Guo, Gaoqiang Li, Shishuai Sun, Shiqi He, Baojing Cheng, Baoming Shi, Anshan Shan
Background: Resveratrol, a plant phenol, affords protection against inflammation and oxidative stress. The objective of this study was to investigate the effects of dietary resveratrol supplementation during pregnancy and lactation on the antioxidant status of sows and piglets and on antioxidant gene expression and pathway in placenta. Methods: Forty sows were allotted to 2 dietary treatments 20 d after breeding. Sows were fed a control diet and a control diet with 300 mg/kg resveratrol...
2018: Journal of Animal Science and Biotechnology
https://www.readbyqxmd.com/read/29702404/mgst1-a-gsh-transferase-peroxidase-essential-for-development-and-hematopoietic-stem-cell-differentiation
#2
Lars Bräutigam, Jie Zhang, Kristian Dreij, Linda Spahiu, Arne Holmgren, Hiroshi Abe, Kenneth D Tew, Danyelle M Townsend, Michael J Kelner, Ralf Morgenstern, Katarina Johansson
We show for the first time that, in contrast to other glutathione transferases and peroxidases, deletion of microsomal glutathione transferase 1 (MGST1) in mice is embryonic lethal. To elucidate why, we used zebrafish development as a model system and found that knockdown of MGST1 produced impaired hematopoiesis. We show that MGST1 is expressed early during zebrafish development and plays an important role in hematopoiesis. High expression of MGST1 was detected in regions of active hematopoiesis and co-expressed with markers for hematopoietic stem cells...
April 16, 2018: Redox Biology
https://www.readbyqxmd.com/read/29695404/establishment-and-characteristics-of-a-novel-mantle-cell-lymphoma-derived-cell-line-and-a-bendamustine-resistant-subline
#3
Tomoko Takimoto-Shimomura, Hisao Nagoshi, Saori Maegawa, Yuto Fujibayashi, Taku Tsukamoto, Yayoi Matsumura-Kimoto, Yoshimi Mizuno, Yoshiaki Chinen, Shinsuke Mizutani, Yuji Shimura, Shigeo Horiike, Masafumi Taniwaki, Tsutomu Kobayashi, Junya Kuroda
BACKGROUND/AIM: Bendamustine hydrochloride (BH) is a key therapeutic agent for mantle cell lymphoma (MCL), while the mechanism underlying BH-resistance has not been verified. MATERIALS AND METHODS: We compared molecular/biological characteristics of a newly-generated MCL-derived cell line KPUM-YY1 and its BH-resistant subline KPUM-YY1R. RESULTS: The growth-inhibitory IC50 for BH was 20 μM in KPUM-YY1 cells, while cell proliferation was not inhibited by up to 60 μM BH in KPUM-YY1R cells...
May 2018: Cancer Genomics & Proteomics
https://www.readbyqxmd.com/read/29611025/expressions-of-some-antioxidant-genes-in-sh-sy5y-cells-treated-with-%C3%AE-lapachone-morphine-and-electromagnetic-field
#4
Hamideh Mahmoudinasab, Mostafa Saadat
β-Lapachone (β-Lap), morphine (Mor), and electromagnetic field (EMF) generate reactive oxygen species. The goal of the present study was to examine the effects of Mor and EMF, in combination with β-Lap on the cell growth inhibition and expression of several antioxidant genes. The 0.50 mT intensity of 50 Hz EMF and two exposure conditions ("15 min field-on/15 min field-off" and "30 min field-on continuously") on SH-SY5Y cells were used. The effects of Mor and EMF, in combination with β-Lap on cell growth inhibition and the expression levels of several antioxidant genes (NQO1, NQO2, SOD1, SOD2, CAT, GSTO1, GSTM2, GSTM3, GSTP1, MGST1, MGST3) in SH-SY5Y cells were measured...
April 2, 2018: Molecular Biology Reports
https://www.readbyqxmd.com/read/29549163/nrf2-mediated-antioxidant-defense-and-peroxiredoxin-6-are-linked-to-biosynthesis-of-palmitic-acid-ester-of-9-hydroxystearic-acid
#5
Ondrej Kuda, Marie Brezinova, Jan Silhavy, Vladimir Landa, Vaclav Zidek, Chandra Dodia, Franziska Kreuchwig, Marek Vrbacky, Laurence Balas, Thierry Durand, Norbert Hübner, Aron B Fisher, Jan Kopecky, Michal Pravenec
Fatty acid esters of hydroxy fatty acids (FAHFAs) are lipid mediators with promising anti-diabetic and anti-inflammatory properties that are formed in white adipose tissue (WAT) via de novo lipogenesis, but their biosynthetic enzymes are unknown. Using a combination of lipidomics in WAT, QTL mapping and correlation analyses in rat BXH/HXB recombinant inbred strains, and response to oxidative stress in murine models, we elucidated the potential pathway of biosynthesis of several FAHFAs.Comprehensive analysis of WAT samples identified ∼160 regioisomers documenting the complexity of this lipid class...
March 16, 2018: Diabetes
https://www.readbyqxmd.com/read/29408927/transcriptional-profiling-of-liver-tissues-in-chicken-embryo-at-day-16-and-20-using-rna-sequencing-reveals-differential-antioxidant-enzyme-activity
#6
Shaohua Yang, Lu Lu Wang, Zhaoyuan Shi, Xiaoqian Ou, Wei Wang, Xue Chen, Guoqing Liu
Considering the high proportion of polyunsaturated fatty acids, the antioxidant defense of chick embryo tissues is vital during the oxidative stress experienced at hatching. In order to better understand the mechanisms of the defense system during chicken embryo development, we detected the activity of antioxidant enzymes during the incubation of chicken embryo. Results showed that the activity of superoxide dismutase (SOD) and (GSH-PX) in livers were higher than those in hearts. Based on these results, liver tissues were used as the follow-up study materials, which were obtained from chicken embryo at day 16 and day 20...
2018: PloS One
https://www.readbyqxmd.com/read/29093368/-development-of-a-fluorescence-probe-for-live-cell-imaging
#7
REVIEW
Aya Shibata
 Probes that detect specific biological materials are indispensable tools for deepening our understanding of various cellular phenomena. In live cell imaging, the probe must emit fluorescence only when a specific substance is detected. In this paper, we introduce a new probe we developed for live cell imaging. Glutathione S-transferase (GST) activity is higher in tumor cells than in normal cells and is involved in the development of resistance to various anticancer drugs. We previously reported the development of a general strategy for the synthesis of probes for detection of GST enzymes, including fluorogenic, bioluminogenic, and 19 F-NMR probes...
2017: Yakugaku Zasshi: Journal of the Pharmaceutical Society of Japan
https://www.readbyqxmd.com/read/29074393/dcf1-subcellular-localization-and-its-function-in-mitochondria
#8
Yanlu Chen, Ruili Feng, Guanghong Luo, Jianjian Guo, Yajiang Wang, Yangyang Sun, Lili Zheng, Tieqiao Wen
Dendritic cell factor 1 (DCF1) is a transmembrane protein that plays important roles in regulating neural stem cell differentiation and dendritic spine formation. Apart from its cytoplasmic functions, DCF1 plays a role in autophagy during the regulation of amyloid precursor proteins. However, the subcellular localization of DCF1 remains unknown. Therefore, in this study, DCF1 tagged with green fluorescent protein was transiently expression in HelaS3 and HEK293T cells. The results showed that DCF1 was widely expressed in different organelles, including the mitochondria, Golgi apparatus, endoplasmic reticulum, endosomes and lysosomes...
January 2018: Biochimie
https://www.readbyqxmd.com/read/28938523/hypothermia-induced-by-anesthesia-regulates-various-signals-expressions-in-the-hippocampus-of-animals
#9
Huimei Hao, Shanshan Wang
Though important for a variety of medical procedures, general anesthesia is not a problem free. Some anesthetics have been suggested to affect a number of signals, which are associated with memory consolidation and cognition. In the present study, we attempted to investigate the molecular mechanism of anesthesia-regulated processes. We found that under hypothermic condition, anesthetic of isoflurane enhances various signals expression in hippocampus, including Hspd1, Actb, Mgst1, THBS4, Syp, C1QC, Serpine, Plat, and Ngf, which were related to cellular stress, neural plasticity responses, and hippocampal injury...
November 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/28923017/within-breed-and-multi-breed-gwas-on-imputed-whole-genome-sequence-variants-reveal-candidate-mutations-affecting-milk-protein-composition-in-dairy-cattle
#10
Marie-Pierre Sanchez, Armelle Govignon-Gion, Pascal Croiseau, Sébastien Fritz, Chris Hozé, Guy Miranda, Patrice Martin, Anne Barbat-Leterrier, Rabia Letaïef, Dominique Rocha, Mickaël Brochard, Mekki Boussaha, Didier Boichard
BACKGROUND: Genome-wide association studies (GWAS) were performed at the sequence level to identify candidate mutations that affect the expression of six major milk proteins in Montbéliarde (MON), Normande (NOR), and Holstein (HOL) dairy cattle. Whey protein (α-lactalbumin and β-lactoglobulin) and casein (αs1, αs2, β, and κ) contents were estimated by mid-infrared (MIR) spectrometry, with medium to high accuracy (0.59 ≤ R2  ≤ 0.92), for 848,068 test-day milk samples from 156,660 cows in the first three lactations...
September 18, 2017: Genetics, Selection, Evolution: GSE
https://www.readbyqxmd.com/read/28835686/genome-wide-comparative-analyses-of-correlated-and-uncorrelated-phenotypes-identify-major-pleiotropic-variants-in-dairy-cattle
#11
Ruidong Xiang, Iona M MacLeod, Sunduimijid Bolormaa, Michael E Goddard
While single nucleotide polymorphisms (SNPs) associated with multiple phenotype have been reported, the knowledge of pleiotropy of uncorrelated phenotype is minimal. Principal components (PCs) and uncorrelated Cholesky transformed traits (CT) were constructed using 25 raw traits (RTs) of 2841 dairy bulls. Multi-trait meta-analyses of single-trait genome-wide association studies for RT, PC and CT in bulls were validated in 6821 cows. Most PCs and CTs had substantial estimates of heritability, suggesting that genes affect phenotype via diverse pathways...
August 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28801553/dead-end-complex-lipid-interactions-and-catalytic-mechanism-of-microsomal-glutathione-transferase-1-an-electron-crystallography-and-mutagenesis-investigation
#12
Qie Kuang, Pasi Purhonen, Johan Ålander, Richard Svensson, Veronika Hoogland, Jens Winerdal, Linda Spahiu, Astrid Ottosson-Wadlund, Caroline Jegerschöld, Ralf Morgenstern, Hans Hebert
Microsomal glutathione transferase 1 (MGST1) is a detoxification enzyme belonging to the Membrane Associated Proteins in Eicosanoid and Glutathione Metabolism (MAPEG) superfamily. Here we have used electron crystallography of two-dimensional crystals in order to determine an atomic model of rat MGST1 in a lipid environment. The model comprises 123 of the 155 amino acid residues, two structured phospholipid molecules, two aliphatic chains and one glutathione (GSH) molecule. The functional unit is a homotrimer centered on the crystallographic three-fold axes of the unit cell...
August 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28558199/global-kinetic-mechanism-of-microsomal-glutathione-transferase-1-and-insights-into-dynamic-enzyme-activation
#13
Linda Spahiu, Johan Ålander, Astrid Ottosson-Wadlund, Richard Svensson, Carina Lehmer, Richard N Armstrong, Ralf Morgenstern
Microsomal glutathione transferase 1 (MGST1) has a unique ability to be activated, ≤30-fold, by modification with sulfhydryl reagents. MGST1 exhibits one-third-of-the-sites reactivity toward glutathione and hence heterogeneous binding to different active sites in the homotrimer. Limited turnover stopped-flow kinetic measurements of the activated enzyme allowed us to more accurately determine the KD for the "third" low-affinity GSH binding site (1.4 ± 0.3 mM). The rate of thiolate formation, k2 (0...
June 20, 2017: Biochemistry
https://www.readbyqxmd.com/read/28450942/immunohistochemical-analysis-of-microsomal-glutathione-s-transferase-1-and-clusterin-expression-in-lens-epithelial-cells-of-patients-with-pseudoexfoliation-syndrome
#14
Joanna Stafiej, Marta Hałas-Wiśniewska, Magdalena Izdebska, Maciej Gagat, Dariusz Grzanka, Alina Grzanka, Grażyna Malukiewicz
Pseudoexfoliation syndrome (PEX) is an age-associated, sight disorder affecting elastic fibers in the eye and visceral organs but its exact etiology remains unknown. The purpose of the current study was to determine the morphology and ultrastructure of lens epithelial cells (LECs), and to use immunohistochemistry to examine localization of microsomal glutathione S-transferase 1 (MGST1) and clusterin. Anterior lens capsules were obtained from 24 patients (13 PEX and 11 controls) who underwent phacoemulsification...
March 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/28264803/maternal-and-infant-genetic-variants-maternal-periconceptional-use-of-selective-serotonin-reuptake-inhibitors-and-risk-of-congenital-heart-defects-in-offspring-population-based-study
#15
Wendy N Nembhard, Xinyu Tang, Zhuopei Hu, Stewart MacLeod, Zachary Stowe, Daniel Webber
Objective To evaluate whether the association between maternal periconceptional use of selective serotonin reuptake inhibitors (SSRIs) and increased risk of congenital heart defects in offspring is modified by maternal or infant genetic variants in folate, homocysteine, or transsulfuration pathways.Design Population based study. DNA from mothers, fathers, and infants was genotyped with an Illumina GoldenGate custom single nucleotide polymorphism panel. A hybrid design based on a log linear model was used to calculate relative risks and Bayesian false discovery probabilities (BFDP) to identify polymorphisms associated with congenital heart defects modified by SSRI use...
March 6, 2017: BMJ: British Medical Journal
https://www.readbyqxmd.com/read/28099509/a-comparative-study-on-antioxidant-system-in-fish-hepatopancreas-and-intestine-affected-by-choline-deficiency-different-change-patterns-of-varied-antioxidant-enzyme-genes-and-nrf2-signaling-factors
#16
COMPARATIVE STUDY
Pei Wu, Yang Liu, Wei-Dan Jiang, Jun Jiang, Juan Zhao, Yong-An Zhang, Xiao-Qiu Zhou, Lin Feng
The liver and intestine are susceptible to the oxidative damage which could result in several diseases. Choline deficiency induced oxidative damage in rat liver cells. Thus, this study aimed to investigate the potential molecular mechanisms responsible for choline deficiency-induced oxidative damage. Juvenile Jian carp were fed diets differing in choline content [165 (deficient group), 310, 607, 896, 1167 and 1820 mg/kg diet] respectively for 65 days. Oxidative damage, antioxidant enzyme activities and related gene expressions in the hepatopancreas and intestine were measured...
2017: PloS One
https://www.readbyqxmd.com/read/27913278/molecular-basis-for-the-dual-subcellular-distribution-of-microsomal-glutathione-transferase-1
#17
Miyuki Shimoji, Ricardo A Figueroa, Etienne Neve, Danuta Maksel, Gabriela Imreh, Ralf Morgenstern, Einar Hallberg
Microsomal glutathione transferase 1 (MGST1) is a membrane bound enzyme involved in the detoxification of reactive electrophiles and protection of membranes from oxidative stress. The enzyme displays an unusual and broad subcellular distribution with especially high levels in the endoplasmic reticulum (ER) and outer mitochondrial membrane (OMM). Here we examined the molecular basis for this dual distribution. We hypothesized that the amphipathic properties of the first transmembrane segment (TMS), that contains a positively charged lysine (K25), is a central feature guiding dual targeting...
February 2017: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/27535962/analysis-of-the-uterine-epithelial-and-conceptus-transcriptome-and-luminal-fluid-proteome-during-the-peri-implantation-period-of-pregnancy-in-sheep
#18
Kelsey Brooks, Gregory W Burns, Joao G N Moraes, Thomas E Spencer
Studies support the idea that uterine epithelia and their secretions have important biological roles in conceptus survival, elongation, and implantation in sheep. The present study evaluated the transcriptome of the uterine luminal epithelium (LE) and glandular epithelium (GE) and the conceptus and proteome of uterine luminal fluid (ULF) during the peri-implantation period of pregnancy. Transcriptome (RNA-sequencing) analysis was conducted in LE and GE isolated from uteri of Day 10, 12, 14, 16, and 20 pregnant sheep by laser capture microdissection...
October 2016: Biology of Reproduction
https://www.readbyqxmd.com/read/27486097/germline-variation-in-inflammation-related-pathways-and-risk-of-barrett-s-oesophagus-and-oesophageal-adenocarcinoma
#19
Matthew F Buas, Qianchuan He, Lisa G Johnson, Lynn Onstad, David M Levine, Aaron P Thrift, Puya Gharahkhani, Claire Palles, Jesper Lagergren, Rebecca C Fitzgerald, Weimin Ye, Carlos Caldas, Nigel C Bird, Nicholas J Shaheen, Leslie Bernstein, Marilie D Gammon, Anna H Wu, Laura J Hardie, Paul D Pharoah, Geoffrey Liu, Prassad Iyer, Douglas A Corley, Harvey A Risch, Wong-Ho Chow, Hans Prenen, Laura Chegwidden, Sharon Love, Stephen Attwood, Paul Moayyedi, David MacDonald, Rebecca Harrison, Peter Watson, Hugh Barr, John deCaestecker, Ian Tomlinson, Janusz Jankowski, David C Whiteman, Stuart MacGregor, Thomas L Vaughan, Margaret M Madeleine
OBJECTIVE: Oesophageal adenocarcinoma (OA) incidence has risen sharply in Western countries over recent decades. Local and systemic inflammation is considered an important contributor to OA pathogenesis. Established risk factors for OA and its precursor, Barrett's oesophagus (BE), include symptomatic reflux, obesity and smoking. The role of inherited genetic susceptibility remains an area of active investigation. Here, we explore whether germline variation related to inflammatory processes influences susceptibility to BE/OA...
October 2017: Gut
https://www.readbyqxmd.com/read/27424321/up-regulation-of-glutathione-related-genes-enzyme-activities-and-transport-proteins-in-human-cervical-cancer-cells-treated-with-doxorubicin
#20
Ewa Drozd, Jolanta Krzysztoń-Russjan, Jadwiga Marczewska, Janina Drozd, Irena Bubko, Magda Bielak, Katarzyna Lubelska, Katarzyna Wiktorska, Zdzisław Chilmonczyk, Elżbieta Anuszewska, Beata Gruber-Bzura
Doxorubicin (DOX), one of the most effective anticancer drugs, acts in a variety of ways including DNA damage, enzyme inhibition and generation of reactive oxygen species. Glutathione (GSH) and glutathione-related enzymes including: glutathione peroxidase (GPX), glutathione reductase (GSR) and glutathione S-transferases (GST) may play a role in adaptive detoxification processes in response to the oxidative stress, thus contributing to drug resistance phenotype. In this study, we investigated effects of DOX treatment on expression and activity of GSH-related enzymes and multidrug resistance-associated proteins in cultured human cervical cancer cells displaying different resistance against this drug (HeLa and KB-V1)...
October 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
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