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Sputum eosinophil

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https://www.readbyqxmd.com/read/28528200/sputum-transcriptomics-reveal-up-regulation-of-il-1-receptor-family-members-in-severe-asthma
#1
Christos Rossios, Stelios Pavlidis, Uruj Hoda, Chih-Hsi Kuo, Coen Wiegman, Kirsty Russell, Kai Sun, Matthew J Loza, Frederic Baribaud, Andrew L Durham, Oluwaseun Ojo, Rene Lutter, Anthony Rowe, Aruna Bansal, Charles Auffray, Ana Sousa, Julie Corfield, Ratko Djukanovic, Yike Guo, Peter J Sterk, Kian Fan Chung, Ian M Adcock
BACKGROUND: Sputum analysis in asthma is used to define airway inflammatory processes and may guide therapy. OBJECTIVE: To determine differential gene and protein expression in sputum samples from patients with severe asthma (SA) compared to mild-moderate non-smoking asthmatics (MMA). METHODS: Induced sputum was obtained from non-smoking SA (SAn), smokers/ex-smokers with SA (SAsm), MMA and healthy non-smoking controls. Differential cell counts, microarray analysis of cell pellets and SOMAscan analysis of sputum analytes was performed...
May 17, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28527151/association-of-the-mir-196a2-mir-146a-and-mir-499-polymorphisms-with-asthma-phenotypes-in-a-korean-population
#2
Hoang Kim Tu Trinh, Duy Le Pham, Su-Chin Kim, Ri-Yeon Kim, Hae-Sim Park, Seung-Hyun Kim
BACKGROUND: MicroRNAs (miRNAs) modulate expressions of inflammatory genes, thereby regulating inflammatory responses. Single nucleotide polymorphisms (SNPs) in miRNAs could affect their efficiency in binding to messenger RNAs (mRNAs). OBJECTIVE: We investigated the associations of miRNA SNPs with asthma phenotypes. miR-196a2 (rs11614913 T>C), miR-146a (rs2910164 C>G), and miR-499 (rs3746444 A>G) were genotyped in 347 asthma patients and 172 normal healthy controls (NCs)...
May 19, 2017: Molecular Diagnosis & Therapy
https://www.readbyqxmd.com/read/28497926/plasma-lte4-pgf2%C3%AE-ratio-and-blood-eosinophil-count-are-increased-in-elderly-asthmatics-with-previous-asthma-exacerbation
#3
Ga Young Ban, Young Min Ye, Sang Ha Kim, Gyu Young Hur, Joo Hee Kim, Jae Jung Shim, Kumsun Cho, Joo Youn Cho, Hae Sim Park
The tools for asthma control assessment recommended by the current guideline are cognitive function- and effort-dependent, which is substantially impaired in the elderly. The aim of this study is to investigate objective assessment tools of asthma control status and previous asthma exacerbation (AE) in elderly subjects. Asthmatics aged >60 years who were treated with step 2 or 3 by the Global Initiative for Asthma (GINA) guideline were enrolled. During the 12-week study period, the subjects used either 400 μg of budesonide plus 10 mg of montelukast or 800 μg of inhaled budesonide...
July 2017: Allergy, Asthma & Immunology Research
https://www.readbyqxmd.com/read/28490872/correlation-between-fractional-exhaled-nitric-oxide-and-sputum-eosinophilia-in-exacerbations-of-copd
#4
Jie Gao, Min Zhang, Liqin Zhou, Xing Yang, Haigui Wu, Jianfang Zhang, Feng Wu
INTRODUCTION: Measurements of eosinophils in induced sputum and fractional exhaled nitric oxide (FeNO) are noninvasive biomarkers for assessing airway inflammation phenotypes in chronic obstructive pulmonary disease (COPD). Nevertheless, the clinical application of the correlation between FeNO levels and sputum eosinophilia is controversial. The study aimed to investigate the correlation and predictive relationship between FeNO levels and sputum eosinophils in patients with COPD exacerbation...
2017: International Journal of Chronic Obstructive Pulmonary Disease
https://www.readbyqxmd.com/read/28482840/blood-and-sputum-eosinophils-in-copd-relationship-with-bacterial-load
#5
Umme Kolsum, Gavin C Donaldson, Richa Singh, Bethan L Barker, Vandana Gupta, Leena George, Adam J Webb, Sarah Thurston, Anthony J Brookes, Timothy D McHugh, Jadwiga A Wedzicha, Christopher E Brightling, Dave Singh
BACKGROUND: Sputum and blood eosinophil counts predict corticosteroid effects in COPD patients. Bacterial infection causes increased airway neutrophilic inflammation. The relationship of eosinophil counts with airway bacterial load in COPD patients is uncertain. We tested the hypothesis that bacterial load and eosinophil counts are inversely related. METHODS: COPD patients were seen at stable state and exacerbation onset. Sputum was processed for quantitative polymerase chain reaction detection of the potentially pathogenic microorganisms (PPM) H...
May 8, 2017: Respiratory Research
https://www.readbyqxmd.com/read/28479329/inflammatory-phenotypes-in-severe-asthma-are-associated-with-distinct-airway-microbiology
#6
Steven L Taylor, Lex E X Leong, Jocelyn M Choo, Steve Wesselingh, Ian A Yang, John W Upham, Paul N Reynolds, Sandra Hodge, Alan L James, Christine Jenkins, Matthew J Peters, Melissa Baraket, Guy B Marks, Peter G Gibson, Jodie L Simpson, Geraint B Rogers
BACKGROUND: Asthma pathophysiology and treatment responsiveness are predicted by inflammatory phenotype. However, the relationship between airway microbiology and asthma phenotype is poorly understood. We aimed to characterise airway microbiota in patients with symptomatic stable asthma, and relate composition to airway inflammatory phenotype and other phenotypic characteristics. METHODS: The microbial composition of induced sputum specimens collected from adult patients screened for a multicenter randomized controlled trial was determined by 16S rRNA gene sequencing...
May 4, 2017: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28474652/correlation-of-total-serum-immunoglobulin-e-level-sputum-and-peripheral-eosinophil-count-in-assessing-the-clinical-severity-in-bronchial-asthma
#7
Roshan M Kumar, R Pajanivel, G Koteeswaran, Surendra K Menon, Pravin Mv Charles
CONTEXT: Asthma is a chronic inflammatory disorder of the airway with involvement of various cellular populations and release of many inflammatory mediators. Eosinophils and serum immunoglobulin E (IgE) are considered a good marker of airway inflammation in asthma. The correlation of clinical assessment with various markers of airway inflammation in asthma is not well established in the Indian population. AIMS: This study aims to study the correlation of serum IgE, sputum eosinophil count, and peripheral eosinophil count with clinical severity of Asthma...
May 2017: Lung India: Official Organ of Indian Chest Society
https://www.readbyqxmd.com/read/28474352/airway-basophils-are-increased-and-activated-in-eosinophilic-asthma
#8
Yoshihiro Suzuki, Keiko Wakahara, Tomoko Nishio, Satoru Ito, Yoshinori Hasegawa
BACKGROUND: The impact of basophils on asthma pathogenesis remains largely unexplored, particularly in humans. Here, we evaluated the frequencies and activation status of basophils in the sputum of adult asthmatic patients and related our findings to other parameters of eosinophilic airway inflammation. METHODS: We enrolled 44 adult asthmatic patients who were being treated with inhaled corticosteroids (ICS). Analysis of the induced sputum, exhaled nitric oxide fraction (FeNO) measurement, and Asthma Control Test (ACT) were carried out together with standard blood and pulmonary function tests...
May 5, 2017: Allergy
https://www.readbyqxmd.com/read/28466576/reference-value-of-induced-sputum-cell-counts-and-its-relationship-with-age-in-healthy-adults-in-guangzhou-southern-china
#9
Wei Luo, Qiaoli Chen, Ruchong Chen, Yanqing Xie, Hui Wang, Kefang Lai
PURPOSE: To establish the reference value of total and differential cell counts in induced sputum in healthy adults in Guangzhou, and to explore the relationship of age and gender with sputum cell counts. METHODS: A total of 185 healthy, nonatopic, never-smokers between 19 and 74 years old underwent induced sputum test with hypertonic saline (3%) inhalation. Sputum samples were collected for total and differential cell counts. RESULTS: Qualified sputum samples were obtained from 153 subjects (mean age: 43±17) with a success rate of 82...
May 2, 2017: Clinical Respiratory Journal
https://www.readbyqxmd.com/read/28461299/th-2-signature-in-chronic-airway-diseases-towards-the-extinction-of-asthma-copd-overlap-syndrome
#10
Borja G Cosío, Luis Pérez de Llano, Antolin Lopez Viña, Alfons Torrego, Jose Luis Lopez-Campos, Joan B Soriano, Eva Martinez Moragon, Jose Luis Izquierdo, Irina Bobolea, Javier Callejas, Vicente Plaza, Marc Miravitlles, Juan Jose Soler-Catalunya
We aimed to describe the differences and similarities between patients with chronic obstructive airway disease classified on the basis of classical diagnostic labels (asthma, chronic obstructive pulmonary disease (COPD), or asthma-COPD overlap (ACOS)) or according to the underlying inflammatory pattern (Th-2 signature, either Th-2-high or Th-2-low).We performed a cross-sectional study of patients aged ≥40 years and with a post-bronchodilator forced expiratory volume in 1 s to forced vital capacity ratio ≤0...
May 2017: European Respiratory Journal: Official Journal of the European Society for Clinical Respiratory Physiology
https://www.readbyqxmd.com/read/28442899/approaches-to-drug-therapy-for-copd-in-russia-a-proposed-therapeutic-algorithm
#11
Kirill A Zykov, Svetlana I Ovcharenko
Until recently, there have been few clinical algorithms for the management of patients with COPD. Current evidence-based clinical management guidelines can appear to be complex, and they lack clear step-by-step instructions. For these reasons, we chose to create a simple and practical clinical algorithm for the management of patients with COPD, which would be applicable to real-world clinical practice, and which was based on clinical symptoms and spirometric parameters that would take into account the pathophysiological heterogeneity of COPD...
2017: International Journal of Chronic Obstructive Pulmonary Disease
https://www.readbyqxmd.com/read/28438346/post-bronchodilator-reversibility-of-fev1-and-eosinophilic-airway-inflammation-in-copd
#12
Kun-Ta Chou, Kang-Cheng Su, Yi-Han Hsiao, Shiang-Fen Huang, Hsin-Kuo Ko, Ching-Min Tseng, Vincent Yi-Fong Su, Diahn-Warng Perng
INTRODUCTION: The relationship between bronchodilator responsiveness and eosinophilic airway inflammation has not been well documented in COPD. It has been investigated in this retrospective study. This issue has grown in importance due to increasing interest in the asthma-COPD overlap syndrome. METHODS: 264 stable COPD patients with no past history of asthma were retrospectively analyzed. Correlation analyses between FEV1 reversibility and sputum eosinophil levels were conducted...
April 21, 2017: Archivos de Bronconeumología
https://www.readbyqxmd.com/read/28427296/predictors-of-response-to-therapy-with-omalizumab-in-patients-with-severe-allergic-asthma-a-real-life-study
#13
Maria Kallieri, Andriana I Papaioannou, Evgenia Papathanasiou, Polyxeni Ntontsi, Spyridon Papiris, Stelios Loukides
OBJECTIVES: Omalizumab is a recombinant humanized IgG1 monoclonal anti-IgE antibody, used for the treatment of severe refractory allergic asthma. However, not all patients with IgE levels within the limits of administration, respond to treatment. The aim of the present study, was to determine clinical and inflammatory characteristics that could predict response to omalizumab. METHODS: We studied retrospectively patients treated with omalizumab as per GINA guidelines in one asthma tertiary referral center...
April 28, 2017: Postgraduate Medicine
https://www.readbyqxmd.com/read/28426171/sputum-basophils-are-increased-in-eosinophilic-asthma-compared-with-non-eosinophilic-asthma-phenotypes
#14
Collin R Brooks, Christine J van Dalen, Ian F Hermans, Peter G Gibson, Jodie L Simpson, Jeroen Douwes
Sputum basophil numbers are increased in allergic asthmatics, but it is unclear what role airway basophils play in "TH2-low" asthma phenotypes. Using flow cytometry we found that basophils were significantly increased in all asthmatics (n=26) compared with healthy controls (n=8) (p=0.007) with highest levels observed in eosinophilic asthma (EA; median 0.22%, IQR 0.11-0.47%; n=14) compared with non-EA (0.06%, 0.00-0.20%; n=12; p<0.05). In asthmatics, basophils were positively correlated with sputum eosinophils (r=0...
April 20, 2017: Allergy
https://www.readbyqxmd.com/read/28422515/allergen-induced-increases-in-sputum-levels-of-group-2-innate-lymphoid-cells-in-asthmatic-subjects
#15
Ruchong Chen, Steven G Smith, Brittany Salter, Amani El-Gammal, John Paul Oliveria, Caitlin Obminski, Rick Watson, Paul M O'Byrne, Gail M Gauvreau, Roma Sehmi
RATIONALE: Group 2 innate lymphoid cells (ILC2), a major source of type 2 cytokines, initiate eosinophilic inflammatory responses in murine models of asthma. OBJECTIVES: Investigate the role of ILC2 in allergen-induced airway eosinophilic responses in atopic asthmatics. METHODS: Using a diluent-controlled allergen challenge cross-over study, where all subjects (n=10) developed allergen-induced early and late responses , airway eosinophilia and increased methacholine airway responsiveness, bone marrow, blood and sputum samples were collected pre- and post-inhalation challenge...
April 19, 2017: American Journal of Respiratory and Critical Care Medicine
https://www.readbyqxmd.com/read/28407269/clinical-functional-and-inflammatory-characteristics-in-patients-with-paucigranulocytic-stable-asthma-comparison-with-different-sputum-phenotypes
#16
P Ntontsi, S Loukides, P Bakakos, K Kostikas, G Papatheodorou, E Papathanassiou, G Hillas, N Koulouris, S Papiris, A I Papaioannou
BACKGROUND: According to induced sputum cell count, four different asthma phenotypes have been recognized (eosinophilic, neutrophilic, mixed and paucigranulocytic). The aim of this study was to detect functional and inflammatory characteristics of patients with paucigranulocytic asthma. METHODS: A total of 240 asthmatic patients were categorized into the four phenotypes according to cell counts in induced sputum. All patients underwent pulmonary function tests, and measurement of fraction of exhaled nitric oxide (FeNO)...
April 13, 2017: Allergy
https://www.readbyqxmd.com/read/28396690/sputum-cell-counts-to-manage-prednisone-dependent-asthma-effects-on-fev1-and-eosinophilic-exacerbations
#17
Afia Aziz-Ur-Rehman, Angira Dasgupta, Melanie Kjarsgaard, Frederick E Hargreave, Parameswaran Nair
BACKGROUND: Prednisone dependence in asthma is usually described based on clinical and spirometric characteristics. It is generally believed that these patients have frequent exacerbations and lose lung function rapidly because of uncontrolled airway eosinophilia. OBJECTIVES: The objectives of this study are to report the effect on asthma exacerbations and the change in lung function over time in prednisone-dependent asthma when severe asthma is managed using a protocol that aims to maintain normal sputum cell counts...
2017: Allergy, Asthma, and Clinical Immunology
https://www.readbyqxmd.com/read/28390083/high-fractional-exhaled-nitric-oxide-and-sputum-eosinophils-are-associated-with-an-increased-risk-of-future-virus-induced-exacerbations-ndash-a-prospective-cohort-study
#18
Asger Bjerregaard, Ingrid A Laing, Vibeke Backer, Asger Sverrild, Siew-Kim Khoo, Glenys Chidlow, Chisha Sikazwe, David W Smith, Peter Le Souëf, Celeste Porsbjerg
BACKGROUND: The major trigger of asthma exacerbations is infection with a respiratory virus, most commonly rhinovirus. Type 2 inflammation is known to be associated with an increased risk of exacerbations in general. Whether type 2 inflammation at baseline increases the risk of future virus-induced exacerbations is unknown. OBJECTIVE: To assess whether type 2 inflammation is associated with an increased risk of virus-induced exacerbations of asthma. METHODS: Stable asthmatics had spirometry, skin prick test, measurement of FeNO and sputum induced for differential cell counts...
April 8, 2017: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/28389300/asthma-control-and-sputum-eosinophils-a-longitudinal-study-in-daily-practice
#19
Sophie F Demarche, Florence N Schleich, Virginie A Paulus, Monique A Henket, Thierry J Van Hees, Renaud E Louis
BACKGROUND: Longitudinal trials have suggested that asthma control may be influenced by fluctuations in eosinophilic inflammation. This association has however never been confirmed in daily practice. OBJECTIVE: To investigate the relationship between asthma control and sputum eosinophils in clinical practice. METHODS: A retrospective longitudinal study was conducted on 187 patients with asthma with at least 2 successful sputum inductions at our Asthma Clinic...
April 4, 2017: Journal of Allergy and Clinical Immunology in Practice
https://www.readbyqxmd.com/read/28353595/eotaxin-and-il-4-levels-are-increased-in-induced-sputum-and-correlate-with-sputum-eosinophils-in-patients-with-nonasthmatic-eosinophilic-bronchitis
#20
Rui Zhang, Wei Luo, Zhenyu Liang, Yaxia Tan, Ruchong Chen, Wenju Lu, Nanshan Zhong
Nonasthmatic eosinophilic bronchitis (NAEB) is characterized by chronic cough and airway eosinophilic inflammation. Airway and systemic inflammation cytokine profile have not been comprehensively described in patients with NAEB.The aim of the study was to identify the cytokine profile in sputum and serum of NAEB patients. Furthermore, the relationship between cytokines and clinical features would be evaluated.Induced sputum and serum were collected from untreated NAEB patients and healthy subjects. The cytokine profile in sputum and serum was analyzed by a bead-based multiplex cytokine assay including 21 cytokines...
March 2017: Medicine (Baltimore)
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