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Sputum eosinophil

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https://www.readbyqxmd.com/read/29758111/genetic-profiles-of-transcriptomic-clusters-of-childhood-asthma-determine-specific-severe-subtype
#1
Y-L Yeh, M-W Su, B-L Chiang, Y-H Yang, C-H Tsai, Y L Lee
BACKGROUND: Previous studies have defined transcriptomic subtypes of adult asthma using samples of induced sputum and bronchial epithelium; however, those procedures are not readily applicable in the clinic, especially for childhood asthma. OBJECTIVE: We aim to dissect the transcriptomic clusters of childhood asthma using highly variably expressed genes of peripheral blood mononuclear cells (PBMC) among patients. METHODS: Gene expression of PBMC from 133 asthmatic children and 11 healthy controls was measured with Illumina microarrays...
May 14, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29754974/implication-of-fraction-of-exhaled-nitric-oxide-and-blood-eosinophil-count-in-severe-asthma
#2
Tomoyuki Soma, Hidetoshi Iemura, Erika Naito, Sachiko Miyauchi, Yoshitaka Uchida, Kazuyuki Nakagome, Makoto Nagata
BACKGROUND: Severe asthma is a complex disease with heterogeneous features and involves type 2 airway inflammation, including eosinophil accumulation. Surrogate biomarkers, fraction of exhaled nitric oxide (FeNO) and blood eosinophil count (b-EOS), may predict eosinophilic airway inflammation. Here we investigated clinical characteristics of severe asthma phenotype using a combined analysis of FeNO and b-EOS. METHODS: This retrospective study examined clinical data of patients with severe asthma (N = 107; median age, 64 years) treated at Saitama Medical University Hospital from 2009 to 2016...
May 10, 2018: Allergology International: Official Journal of the Japanese Society of Allergology
https://www.readbyqxmd.com/read/29754456/pentraxin-levels-in-noneosinophilic-versus-eosinophilic-asthma
#3
Pengfei Gao, Kun Tang, Meijia Wang, Qun Yang, Yongjian Xu, Jianmiao Wang, Jianping Zhao, Jungang Xie
BACKGROUND: Innate immunity has been thought to be involved in asthma pathogenesis. Pentraxins, acting as soluble pattern recognition molecules, play an important role in humoral innate immunity. Asthma is a heterogeneous inflammatory disease of airways and can be classified as eosinophilic or noneosinophilic asthma. OBJECTIVE: To investigate whether pentraxin levels differ in subjects with eosinophilic versus noneosinophilic asthma. Furthermore, to access the predictive performance of pentraxin levels for discriminating asthma inflammatory phenotypes...
May 13, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29719412/acute-effects-of-smoke-exposure-on-airway-and-systemic-inflammation-in-forest-firefighters
#4
Niki Gianniou, Charilena Giannakopoulou, Efrossini Dima, Matina Kardara, Paraskevi Katsaounou, Alexandros Tsakatikas, Charis Roussos, Nikolaos Koulouris, Nikoletta Rovina
Introduction: The aim of this study was to assess respiratory health and airway and systemic inflammation in professional forest firefighters post firefighting. Methods: A total of 60 firefighters who participated in forest firefighting operations in Greece during 2008 were included in the study. A questionnaire consisting of symptoms and exposure, pulmonary function, atopy, bronchial hyperresponsiveness, and markers of inflammation in induced sputum, serum, and bronchoalveolar lavage (BAL) fluid was assessed...
2018: Journal of Asthma and Allergy
https://www.readbyqxmd.com/read/29709671/biological-exacerbation-clusters-demonstrate-asthma-and-copd-overlap-with-distinct-mediator-and-microbiome-profiles
#5
Michael A Ghebre, Pee Hwee Pang, Sarah Diver, Dhananjay Desai, Mona Bafadhel, Koirobi Haldar, Tatiana Kebadze, Suzanne Cohen, Paul Newbold, Laura Rapley, Joanne Woods, Paul Rugman, Ian D Pavord, Sebastian L Johnston, Michael Barer, Richard D May, Christopher E Brightling
BACKGROUND: Exacerbations of asthma and chronic obstructive pulmonary disease (COPD) are heterogeneous. OBJECTIVE: We sought to investigate the sputum cellular, mediator, and microbiome profiles of both asthma and COPD exacerbations. METHODS: Patients with severe asthma or moderate-to-severe COPD were prospectively recruited to a single centre. Sputum mediators were available in 32 asthma and 73 COPD patients assessed at exacerbation. Biologic clusters were determined using factor and cluster analyses on a panel of sputum mediators...
April 27, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29696728/predictive-value-of-eosinophils-and-neutrophils-on-clinical-effects-of-ics-in-copd
#6
Floor J Hartjes, Judith M Vonk, Alen Faiz, Pieter S Hiemstra, Thérèse S Lapperre, Huib A M Kerstjens, Dirkje S Postma, Maarten van den Berge
BACKGROUND AND OBJECTIVE: Inflammation is present to a variable degree and composition in patients with COPD. This study investigates associations between both eosinophils and neutrophils in blood, sputum, airway wall biopsies and bronchoalveolar lavage (BAL) and their potential use as biomarkers for clinical response to inhaled corticosteroids (ICS). METHODS: In total, 114 steroid-naïve COPD patients of the Groningen Leiden Universities Corticosteroids in Obstructive Lung Disease (GLUCOLD) study using ICS or placebo during 30-month follow-up were included...
April 26, 2018: Respirology: Official Journal of the Asian Pacific Society of Respirology
https://www.readbyqxmd.com/read/29694974/il-33-and-its-receptor-st2-after-inhaled-allergen-challenge-in-allergic-asthmatics
#7
Patrick D Mitchell, Brittany M Salter, John Paul Oliveria, Amani El-Gammal, Damian Tworek, Steve G Smith, Roma Sehmi, Gail M Gauvreau, Paul M O Apos Byrne
BACKGROUND: Previous murine models have demonstrated interleukin (IL)-33 to be an important mediator of type-2 inflammation and to promote airway hyperresponsiveness in allergic asthma. A number of inflammatory cells produce IL-33 and eosinophils express ST2 mRNA. The relationship between IL-33 and eosinophils in allergic asthma, however, remains unclear. OBJECTIVE: The aim of this work was to evaluate in vitro the effect of allergen inhalation on IL-33 levels and expression of its receptor (ST2L) on eosinophils in allergic asthmatics, and the effect of IL-33 stimulation on eosinophil activity...
April 25, 2018: International Archives of Allergy and Immunology
https://www.readbyqxmd.com/read/29692617/both-fractional-exhaled-nitric-oxide-and-sputum-eosinophil-were-associated-with-uncontrolled-asthma
#8
Jie Gao, Zhaocheng Chen, Xiang Jie, Ruihua Ye, Feng Wu
Background: Sputum eosinophil and fractional exhaled nitric oxide (FeNO), noninvasive biomarkers of local eosinophilic airway inflammation, can be used to assess asthma outcome. Nevertheless, the clinical application of the association between FeNO and sputum eosinophil is controversial. The aim of the study was to investigate the predictive relationship between FeNO and sputum eosinophil in uncontrolled asthmatic patients and the correlation between sputum eosinophil and FeNO in bronchial reversibility and bronchial hyperresponsiveness (BHR)...
2018: Journal of Asthma and Allergy
https://www.readbyqxmd.com/read/29682796/phase-2a-randomized-double-blind-placebo-controlled-multicenter-parallel-group-study-of-an-h-4-r-antagonist-jnj-39758979-in-adults-with-uncontrolled-asthma
#9
Alexa P Kollmeier, Andrew Greenspan, Xie L Xu, Philip E Silkoff, Elliot Barnathan, Matthew J Loza, Jingzhi Jiang, Bei Zhou, Bin Chen, Robin L Thurmond
BACKGROUND: The effects of H4 R antagonists in preclinical asthma models support the study of antagonists of the H4 R in the treatment of asthma in humans. JNJ-39758979 is a potent and highly-selective oral H4 R antagonist. OBJECTIVE: We sought to evaluate the safety and efficacy of the H4 R-antagonist JNJ-39758979 in adult patients with uncontrolled asthma. METHODS: One hundred and fifteen eligible patients were randomly assigned to JNJ-39758979 300 mg or placebo once daily for 12 weeks in this phase 2a, double-blind, multicenter, placebo-controlled study...
April 23, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29667580/severe-asthma-phenotypes-classified-by-site-of-airway-involvement-and-remodeling-via-chest-ct-scan
#10
S Kim, K Nam Jin, S H Cho, C Hyun Lee, H R Kang
BACKGROUND AND OBJECTIVE: This study aimed to establish a system that can classify severe asthma on the basis of airway remodeling patterns visualized by computed tomography (CT) images and to evaluate the clinical characteristics of individual image-based subtypes. METHODS: Chest CT images of severe asthma patients were retrospectively evaluated to classify phenotypes by site of airway involvement and remodeling. The association between radiologic subtypes and clinical characteristics was assessed...
April 18, 2018: Journal of Investigational Allergology & Clinical Immunology
https://www.readbyqxmd.com/read/29651336/pi3k-p38-and-jak-stat-signalling-in-bronchial-tissue-from-patients-with-asthma-following-allergen-challenge
#11
Thomas Southworth, Sarah Mason, Alan Bell, Isabel Ramis, Marta Calbet, Anna Domenech, Neus Prats, Montserrat Miralpeix, Dave Singh
Background: Inhaled allergen challenges are often used to evaluate novel asthma treatments in early phase clinical trials. Current novel therapeutic targets in asthma include phosphoinositide 3-kinases (PI3K) delta and gamma, p38 mitogen-activated protein kinase (p38) and Janus kinase/Signal Transducer and Activator of Transcription (JAK/STAT) signalling pathways. The activation of these pathways following allergen exposure in atopic asthma patients it is not known. Methods: We collected bronchial biopsies from 11 atopic asthma patients at baseline and after allergen challenge to investigate biomarkers of PI3K, p38 MAPK and JAK/STAT activation by immunohistochemistry...
2018: Biomarker Research
https://www.readbyqxmd.com/read/29644894/decreased-epithelial-and-sputum-mir-221-3p-associates-with-airway-eosinophilic-inflammation-and-cxcl17-expression-in-asthma
#12
Kan Zhang, Yuxia Liang, Yuchen Feng, Wenliang Wu, Huilan Zhang, Jianguo He, Qinghua Hu, Jian-Ping Zhao, Yongjian Xu, Zheng Liu, Guohua Zhen
Airway eosinophilic inflammation is a key feature of type 2-high asthma. The role of epithelial miRNA in airway eosinophilic inflammation remains unclear. We examined the expression of miR-221-3p in bronchial brushings, induced sputum and plasma from 77 symptomatic, recently diagnosed steroid-naïve subjects with asthma and 36 healthy controls by quantitative PCR, and analyzed the correlation between miR-221-3p expression and airway eosinophilia. We found that epithelial, sputum and plasma miR-221-3p expression was significantly decreased in subjects with asthma...
April 12, 2018: American Journal of Physiology. Lung Cellular and Molecular Physiology
https://www.readbyqxmd.com/read/29615049/a-gata3-specific-dnazyme-attenuates-sputum-eosinophilia-in-eosinophilic-copd-patients-a-feasibility-randomized-clinical-trial
#13
Timm Greulich, Jens M Hohlfeld, Petra Neuser, Katrin Lueer, Andreas Klemmer, Carmen Schade-Brittinger, Susanne Harnisch, Holger Garn, Harald Renz, Ursula Homburg, Jonas Renz, Anne Kirsten, Frauke Pedersen, Meike Müller, Claus F Vogelmeier, Henrik Watz
BACKGROUND: A subset of COPD-patients presents with eosinophilic airway inflammation. While treatment of asthmatic patients with the GATA3-specific DNAzyme SB010 attenuated sputum eosinophilia after allergen challenge, this specific treatment has not been evaluated in patients with COPD. Our objective was to evaluate the feasibility and safety of inhaled SB010 in COPD patients with sputum eosinophilia. METHODS: We conducted a randomized, double-blind, placebo-controlled, multicentre clinical trial in COPD-patients with sputum eosinophilia (≥2...
April 4, 2018: Respiratory Research
https://www.readbyqxmd.com/read/29605195/systemic-and-airway-oxidative-stress-in-competitive-swimmers
#14
Sabina Škrgat, Robert Marčun, Izidor Kern, Mira Šilar, Julij Šelb, Matjaž Fležar, Peter Korošec
BACKGROUND: The environment in swimming pools, which contain chlorine, might interact with the airway epithelium, resulting in oxidative stress and/or inflammation during high intensity training periods. METHODS: We evaluated pulmonary functional (metacholine challenge test, FEV1 and VC), cellular (eosinophils and neutrophils), inflammatory (FeNo, IL-5, IL-6, IL-8 and TNF-α), oxidative (8-isoprostanes) and angiogenesis factors (VEGF) in induced sputum and peripheral blood of 41 healthy non-asthmatic elite swimmers (median 16 years) during the period of high intensity training before a national championship...
April 2018: Respiratory Medicine
https://www.readbyqxmd.com/read/29575717/benefits-of-antifungal-therapy-in-asthma-patients-with-airway-mycosis-a-retrospective-cohort-analysis
#15
Evan Li, Chu-Lin Tsai, Zahida K Maskatia, Ekta Kakkar, Paul Porter, Roger D Rossen, Sarah Perusich, John M Knight, Farrah Kheradmand, David B Corry
INTRODUCTION: Fungal airway infection (airway mycosis) is increasingly recognized as a cause of asthma and related disorders. However, prior controlled studies of patients treated with antifungal antibiotics have produced conflicting results. Our objective is to measure the effect of antifungal therapy in moderate to severe adult asthmatics with positive fungal sputum cultures in a single center referral-based academic practice. METHODS: We retrospectively evaluated 41 patients with asthma and culture-proven airway mycosis treated with either terbinafine, fluconazole, itraconazole, voriconazole, or posaconazole for 4 to >12 weeks together with standard bronchodilator and anti-inflammatory agents...
March 25, 2018: Immunity, Inflammation and Disease
https://www.readbyqxmd.com/read/29553094/role-of-regulatory-t-cells-in-airway-inflammation-in-asthma
#16
Tomotaka Kawayama, Takashi Kinoshita, Kazuko Matsunaga, Yoshiko Naito, Jun Sasaki, Yoshikazu Tominaga, Tomoaki Hoshino
Asthma is an allergic disease characterized by chronic airway inflammation, airway hyperresponsiveness (AHR), reversibility and remodeling. Inhaled corticosteroids (ICS) are effective in many patients with asthma. However, ICS are a controlling, but not but curative treatment, and there are still many patients with refractory and difficult-to-treat asthma. The evaluation of airway inflammation by induced sputum, non-specific AHR by methacholine, and asthmatic reactions by specific allergen challenge techniques are useful not only to investigate the pathogenesis of asthma but also to help develop new drugs for asthma management...
March 16, 2018: Kurume Medical Journal
https://www.readbyqxmd.com/read/29552299/tulobuterol-patch-alleviates-allergic-asthmic-inflammation-by-blockade-of-syk-and-nf-%C3%AE%C2%BAb-activation-in-mice
#17
Lixia Fu, Jing Guan, Yujia Zhang, Pei Ma, Yuanyuan Zhuang, Jinye Bai, Yasi Ding, Qi Hou, Wan Gong, Mingbao Lin, Wensheng Zheng, Jianmin Zhang
Background: Tulobuterol patch, one of strongest bronchodilators, was recently shown to improve bronchial hyperresponsiveness and significantly decrease the sputum eosinophil counts by combining with nonspecific anti-inflammatory drugs on patients with asthma. However, there is limited study on the anti-inflammatory activities of tulobuterol patch and its potential machenism. Results: The tulobuterol patch significantly ameliorated inflammatory cell infiltration in the lung tissue, reduced the number of total leukocytes and its differential count, markedly reduced the production of IL-1β, TNF-α, IL-6, CCL-11 and IL-4 in bronchial alveolar lavage fluid, as well as a reduction in IL-4/IFN-γ ratio...
February 23, 2018: Oncotarget
https://www.readbyqxmd.com/read/29524537/refractory-airway-type-2-inflammation-in-a-large-subgroup-of-asthmatic-patients-treated-with-inhaled-corticosteroids
#18
Michael C Peters, Sheena Kerr, Eleanor M Dunican, Prescott G Woodruff, Merritt L Fajt, Bruce D Levy, Elliot Israel, Brenda R Phillips, David T Mauger, Suzy A Comhair, Serpil C Erzurum, Mats W Johansson, Nizar N Jarjour, Andrea M Coverstone, Mario Castro, Annette T Hastie, Eugene R Bleecker, Sally E Wenzel, John V Fahy
BACKGROUND: Airway type 2 inflammation is usually corticosteroid sensitive, but the role of type 2 inflammation as a mechanism of asthma in patients receiving high-dose inhaled corticosteroids (ICSs) is uncertain. OBJECTIVE: We sought to determine whether airway type 2 inflammation persists in patients treated with ICSs and to evaluate the clinical features of patients with steroid-resistant airway type 2 inflammation. METHODS: We used quantitative PCR to generate a composite metric of type 2 cytokine gene expression (type 2 gene mean [T2GM]) in induced sputum cells from healthy control subjects, patients with severe asthma receiving ICSs (n = 174), and patients with nonsevere asthma receiving ICSs (n = 85)...
March 7, 2018: Journal of Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29520864/complex-association-patterns-for-inflammatory-mediators-in-induced-sputum-from-subjects-with-asthma
#19
A T Hastie, C Steele, C W Dunaway, W C Moore, B M Rector, E Ampleford, H Li, L C Denlinger, N Jarjour, D A Meyers, E R Bleecker
BACKGROUND: The release of various inflammatory mediators into the bronchial lumen is thought to reflect both the type and degree of airway inflammation, eosinophilic Th2, and Th9, or neutrophilic Th1, and Th17, in patients with asthma. AIMS: We investigated whether cytokines and chemokines differed in sputum from subjects with more severe compared with milder asthma and whether unbiased factor analysis of cytokine and chemokine groupings indicates specific inflammatory pathways...
March 9, 2018: Clinical and Experimental Allergy: Journal of the British Society for Allergy and Clinical Immunology
https://www.readbyqxmd.com/read/29518425/leukotriene-e-4-induces-airflow-obstruction-and-mast-cell-activation-via-the-cyslt-1-receptor
#20
Nikolaos Lazarinis, Johan Bood, Cristina Gomez, Johan Kolmert, Ann-Sofie Lantz, Pär Gyllfors, Andy Davis, Craig E Wheelock, Sven-Erik Dahlén, Barbro Dahlén
BACKGROUND: Leukotriene E4 (LTE4 ) is the final active metabolite among the cysteinyl leukotrienes (CysLTs). Animal studies have identified a distinct LTE4 receptor, suggesting that current CysLT1 receptor antagonists may provide incomplete inhibition of CysLT responses. OBJECTIVE: We tested this hypothesis by assessing the influence of the CysLT1 antagonist montelukast on responses induced by inhalation of LTE4 in asthmatic subjects. METHODS: Fourteen subjects with mild intermittent asthma and two subjects with aspirin exacerbated respiratory disease (AERD) received montelukast 20 mg bid and placebo for 5-7 days in a randomized, double blind, crossover study (NCT01841164)...
March 5, 2018: Journal of Allergy and Clinical Immunology
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