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Carba np

Nayeem Ahmad, Shamsi Khalid, Syed M Ali, Asad U Khan
Carbapenem-resistance among enterobacteriaceae has become a global health concern. The objective of this study was to understand NDM producing enterobacteriaceae and their genetic basis of resistance, spreading in neonatal intensive care unit. Carbapenem resistant NDM producing enterobacteriaceae isolates were recovered from rectal swab and blood sample of infants admitted in NICU. These were determined by using Carba-NP test. All isolates were identified using BD PhoenixTM-100 and MICs were determined by broth microdilution method...
2018: Frontiers in Microbiology
Varsha Gupta, Ranu Soni, Neha Jain, Jagdish Chander
The rise in carbapenemases-producing organisms has challenged the scientific community. Infections caused by these bacteria have limited treatment options. There are various types such as Klebsiella pneumoniae carbapenemase (Ambler class A), metallo-beta-lactamases of VIM-type, IMP-type, NDM-type (Ambler class B), and OXA-48-types (Ambler class D). An efficient strategy for detection of carbapenemase producers is important to determine the appropriate therapeutic modalities. In this study, four methods - Carba NP test, modified Carba NP (MCNP) test, carbapenem inactivation method (CIM) test, and Rapidec Carba NP kit test were evaluated...
January 2018: Journal of Laboratory Physicians
Ellionor Rapp, Ørjan Samuelsen, Martin Sundqvist
This study evaluated the performance of the MBT STAR-Carba kit (Bruker Daltonics), to detect carbapenemase producing Enterobacteriaceae, Pseudomonas aeruginosa and Acinetobacter spp. in comparison with the RAPIDEC® CARBA NP test (BioMerieux). MBT STAR-Carba allowed the detection of carbapenemases in Enterobacteriaceae and P. aeruginosa.
January 19, 2018: Journal of Microbiological Methods
Madalina-Maria Muntean, Andrei-Alexandru Muntean, Lauraine Gauthier, Elodie Creton, Garance Cotellon, Mircea Ioan Popa, Rémy A Bonnin, Thierry Naas
Objectives: Fast and accurate diagnostic tests to identify carbapenemase-producing Enterobacteriaceae (CPE) are mandatory for proper antimicrobial therapy and implementing infection control measures. Here, we have developed a rapid Carbapenem Inactivation Method (rCIM) for CPE detection. Methods: The rCIM consists of the incubation of a potential carbapenemase producer with meropenem discs and use of the resulting supernatant to challenge a susceptible indicator strain...
January 16, 2018: Journal of Antimicrobial Chemotherapy
Tomokazu Kuchibiro, Masaru Komatsu, Katsutoshi Yamasaki, Tatsuya Nakamura, Hisaaki Nishio, Isao Nishi, Keigo Kimura, Makoto Niki, Tamotsu Ono, Noriyuki Sueyoshi, Machiko Kita, Kaneyuki Kida, Masanobu Ohama, Kaori Satoh, Hirofumi Toda, Tetsu Mizutani, Nozomi Fukuda, Kana Sawa, Isako Nakai, Tomomi Kofuku, Tamaki Orita, Hideo Watari, Satoshi Shimura, Saori Fukuda, Akihiro Nakamura, Yasunao Wada
Carbapenemase-producing Enterobacteriaceae (CPE) are increasing worldwide. Rapid and accurate detection of CPE is necessary for appropriate antimicrobial treatment and hospital infection control. However, CPE contains some strains that are difficult to detect depending on genotype and MIC value of carbapenem, and a detection method has not been established. The recently reported modified carbapenem inactivation method (mCIM) has been developed in CLSI M100-S27 as a phenotypic technique for detecting carbapenemase activity...
December 13, 2017: Journal of Infection and Chemotherapy: Official Journal of the Japan Society of Chemotherapy
Ohad Shalom, Amos Adler
The goals of the study were to examine the analytic and performance parameters of 2 commercial rapid carbapenem-hydrolysis assays, the β-CARBA test (Bio-Rad) and the Rapid CARB Blue Kit (ROSCO) in comparison with an in-house CARBA NP assay for the detection of Carbapenemase-Producing Enterobacteriaceae (CPE). Their performance was evaluated following growth on 2 chromogenic CPE screening media. The sensitivity was highest (91%) in the β-CARBA test when used from the mSuperCARBA™ plates and was lowest when the same test was used from the chromID™ CARBA plates (75%)...
February 2018: Diagnostic Microbiology and Infectious Disease
Constanza Muñoz, Cecilia Zumarán, Tamara González, Aniela Wozniak, Claudia Castillo, Patricia García
INTRODUCTION: The detection of carbapenemase-producing gram negative bacilli is complicated, because there are available multiple options of test. The confirmation of the enzyme by molecular characterization is not available in all laboratories in our country. OBJECTIVE: To propose a fast, efficient and simple strategy to detect and confirm CPB. MATERIALS AND METHODS: 39 CPB isolates and 8 non-producing were used to evaluate the phenotypic test Carba NP, CarbAcineto NP and Blue-Carba, validating the test Xpert® Carba-R, to be used directly with bacterial colonies with conventional PCR...
August 2017: Revista Chilena de Infectología: órgano Oficial de la Sociedad Chilena de Infectología
Meritxell Garcia-Quintanilla, Laurent Poirel, Patrice Nordmann
A novel chromogenic medium CHROMagar mSuperCARBA was evaluated to detect carbapenem-resistant Gram-negatives. This medium is as sensitive and as specific as the SUPERCARBA medium for detecting KPC, MBL and OXA-48-type producers (100% and 100%, respectively) and is compatible with subsequent testing of carbapenemase activity using the RAPIDEC(®) CARBA NP.
October 19, 2017: Diagnostic Microbiology and Infectious Disease
Patricia J Simner, J Kristie Johnson, William B Brasso, Karen Anderson, David R Lonsway, Virginia M Pierce, April M Bobenchik, Zabrina C Lockett, Angella Charnot-Katsikas, Lars F Westblade, Brian B Yoo, Stephen G Jenkins, Brandi M Limbago, Sanchita Das, Darcie E Roe-Carpenter
The purpose of this study was to develop the modified Carbapenem Inactivation Method (mCIM) for the detection of carbapenemase-producing (CP) Pseudomonas aeruginosa (PA) and Acinetobacter baumannii (AB) and perform a multicenter evaluation of the mCIM and Carba NP tests for these non-fermenters. Thirty P. aeruginosa and 30 A. baumannii isolates previously characterized by whole genome sequencing from the CDC-FDA Antibiotic Resistance Isolate Bank were evaluated, including carbapenemase-producers (CP; Ambler Class A, B, and D), non-carbapenemase-producing (non-CP) carbapenem-resistant isolates, and carbapenem-susceptible isolates...
November 8, 2017: Journal of Clinical Microbiology
Jeremy Surre, Isabelle Canard, Pierrot Bourne-Branchu, Estelle Courbiere, Christine Franceschi, Sonia Chatellier, Alex van Belkum, Mahendrasingh Ramjeet
Screening for the detection of carbapenemase-producing bacteria still encounters issues related to workflow, limit of detection, or qualitative interpretation. We developed a spectrophotometry-based version of the Carba NP phenol red assay (Nordmann et al., 2012) in a microtiter plate format, compatible with low bacterial cell counts. We were able to detect highly active carbapenemases such as KPC and IMP in 30min. A wider range of carbapenemases including OXA-48 were detected using higher inocula, still being competitive compared with currently available phenol red assays...
January 2018: Diagnostic Microbiology and Infectious Disease
Daniel Romero-Alvarez, Jorge Reyes, Viviana Quezada, Carolina Satán, Nelson Cevallos, Sofía Barrera, Gabriel Trueba, Luis E Escobar, José E Villacís
OBJECTIVES: To describe a clinical case of Klebsiella pneumoniae harboring a New Delhi metallo-β-lactamase (NDM) plasmid in Ecuador and to present a map of reports of NDM isolates in South America. METHODS: The modified Hodge test, carbapenem inactivation method, imipenem-EDTA disk method (synergy), and Rapidec Carba NP test were used to identify antibiotic resistance mechanisms. The presence of resistance genes was explored with a conjugation assay, and molecular confirmation of NDM was performed by PCR and DNA sequencing...
December 2017: International Journal of Infectious Diseases: IJID
Pak-Leung Ho, Ya Wang, Cindy Wing-Sze Tse, Kitty Sau-Chun Fung, Vincent Ch-Chung Cheng, Rodney Lee, Wing-Kin To, Raymond Wai-Man Lai, Wei-Kwang Luk, Tak-Lun Que, Dominic Ngai-Chong Tsang
Rapid and accurate detection of carbapenemase-producing Enterobacteriaceae (CPE) is important for preventing their spread in healthcare settings. We compared the performance of the Carba NP test using the CLSI tube method with that using a modified paper strip method for detection of carbapenemase in 390 Enterobacteriaceae isolates. The isolates were identified by Hong Kong's carbapenem-resistant Enterobacteriaceae surveillance program in 2016 and comprised 213 CPE and 177 carbapenemase-negative Enterobacteriaceae Molecular genotype was used as the reference...
October 25, 2017: Journal of Clinical Microbiology
M Choquet, R Guiheneuf, S Castelain, V Cattoir, M Auzou, E Pluquet, V Decroix
Although carbapenemase-producing Enterobacteriaceae (CPE) have become a serious public health issue, their detection remains challenging. The aim of this study was to implement a test based on imipenem hydrolysis by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-ToF MS), using 65 strains producing or not a carbapenemase. Then, we compared its performance to that of the Rapidec Carba NP test using 20 additional strains. The MS-based test effectively discriminated between CPE and other non-carbapenem-susceptible strains compared to the Rapidec Carba NP test (sensitivity 100% and 92%, specificity 94% and 92%, respectively)...
January 2018: European Journal of Clinical Microbiology & Infectious Diseases
Gina Thomson, David Turner, William Brasso, Susan Kircher, Thierry Guillet, Kenneth Thomson
There is an urgent need for rapid, accurate detection and classification of carbapenemases. The current study evaluated the automated BD Phoenix CPO Detect and the manual bioMérieux Rapidec Carba NP tests for meeting these needs. Both tests were challenged with 294 isolates of Enterobacteriaceae spp., Pseudomonas aeruginosa , and Acinetobacter baumannii chosen to provide extreme diagnostic difficulty. Carbapenemases such as KPC, NMC-A, IMI, SME, NDM, SPM, IMP, VIM, and OXA-23, 40, 48, 58, 72, 181, and 232 were produced by 243 isolates and 51 carbapenemase-negative isolates included porin mutants and producers of extended-spectrum β-lactamases (ESBLs), AmpCs, K1, and broad-spectrum β-lactamases...
December 2017: Journal of Clinical Microbiology
Shoorashetty Manohara Rudresh, Giriyapur Siddappa Ravi, Lakshminarayanappa Sunitha, Sadiya Noor Hajira, Ellappan Kalaiarasan, Belgode Narasimha Harish
PURPOSE: Detection of carbapenemases among Gram-negative bacteria (GNB) is important for both clinicians and infection control practitioners. The Clinical and Laboratory Standards Institute recommends Carba NP (CNP) as confirmatory test for carbapenemase production. The reagents required for CNP test are costly and hence the test cannot be performed on a routine basis. The present study evaluates modifications of CNP test for rapid detection of carbapenemases among GNB. MATERIALS AND METHODS: The GNB were screened for carbapenemase production using CNP, CarbAcineto NP (CANP), and modified CNP (mCNP) test...
October 2017: Journal of Laboratory Physicians
Pierre Bogaerts, Thierry Naas, Veroniek Saegeman, Remy A Bonnin, Annette Schuermans, Stéphanie Evrard, Warda Bouchahrouf, Thomas Jove, Didier Tande, Xavier de Bolle, Te-Din Huang, Laurent Dortet, Youri Glupczynski
Objectives: To describe a novel plasmid-borne class D carbapenemase (CHDL) named OXA-427 identified in several Enterobacteriaceae clinical isolates from nine patients in one Belgian hospital. Methods: OXA-427-producing isolates were analysed by an electrochemical imipenem hydrolysis method (BYG Carba test), Carba NP test, conventional phenotypic assays and by molecular methods (PCR, whole sequencing of the OXA-427-encoding plasmid and cloning). The antimicrobial resistance profile of OXA-427 was analysed by expression of the cloned gene in Escherichia coli DH10B and J53...
September 1, 2017: Journal of Antimicrobial Chemotherapy
John Mark Velasco, Maria Theresa Valderama, Trent Peacock, Nirdnoy Warawadee, Kathyleen Nogrado, Fatima Claire Navarro, Domingo Chua, Srijan Apichai, Ruekit Sirigade, Louis R Macareo, Brett Swierczewski
During 2013-2016, we isolated blaNDM- and blaVIM-harboring Enterobacteriaceae and nonfermentative bacteria from patients in the Philippines. Of 130 carbapenem-resistant isolates tested, 45 were Carba NP-positive; 43 harbored blaNDM, and 2 harbored blaVIM. Multidrug-resistant microbial pathogen surveillance and antimicrobial drug stewardship are needed to prevent further spread of New Delhi metallo-β-lactamase variants.
September 2017: Emerging Infectious Diseases
E Literacka, M Herda, A Baraniak, D Żabicka, W Hryniewicz, A Skoczyńska, M Gniadkowski
The aim of this study was to evaluate the Carba NP test (and CarbAcineto) for the detection of carbapenemases in Enterobacteriaceae, Pseudomonas spp. and Acinetobacter spp., and to assess its usefulness in the routine work of the National Reference Centre for Susceptibility Testing (NRCST) in Poland. The evaluation of the Carba NP/CarbAcineto tests was carried out on a group of 81 Enterobacteriaceae, Pseudomonas spp. and Acinetobacter spp. isolates producing KPC-, NDM-, VIM-, IMP- or OXA-48, -23, -24/40, -58-type carbapenemases, and on 26 carbapenemase-negative strains cultivated on a broad panel of microbiological media...
November 2017: European Journal of Clinical Microbiology & Infectious Diseases
Patricia J Simner, Belita N A Opene, Krizia K Chambers, Matthew E Naumann, Karen C Carroll, Pranita D Tamma
Accurate detection of carbapenemase-producing glucose-nonfermenting Gram-negative bacilli (CPNFs), including Pseudomonas aeruginosa and Acinetobacter baumannii, is necessary to prevent their dissemination within health care settings. We performed a method comparison study of 11 phenotypic carbapenemase detection assays to evaluate their accuracy for the detection of CPNFs. A total of 96 carbapenem-resistant glucose-nonfermenting isolates were included, of which 29% produced carbapenemases. All CPNFs were molecularly characterized to identify β-lactamase genes...
September 2017: Journal of Clinical Microbiology
Najla Mathlouthi, Yomna Ben Lamine, Rania Somai, Sophia Bouhalila-Besbes, Sofiane Bakour, Jean-Marc Rolain, Chedly Chouchani
Acinetobacter baumannii is an important opportunistic and multidrug-resistant pathogen responsible for nosocomial infections in health facilities. The aim of this study was to characterize the molecular mechanisms of carbapenem resistance in A. baumannii isolates isolated from Mohamed Kassab Orthopedic Institute in Tunis, Tunisia. Twenty-five imipenem-resistant A. baumannii clinical isolates collected between 2013 and 2016 were identified using API 20NE and were confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS)...
July 10, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
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