Zhimin Lu, Xiaonan Wang, Licai Ma, Leina Dou, Xiangjun Zhao, Jin Tao, Yang Wang, Shaolin Wang, Dejun Liu, Yingbo Shen, Xuezhi Yu, Wenbo Yu, Liangxi Jia, Zhanhui Wang, Jianzhong Shen, Kai Wen
Rapid phenotypic detection assays, including Carba NP and its variants, are widely applied for clinical diagnosis of carbapenemase-producing Enterobacterales (CPE). However, these tests are based on the acidification of the pH indicator during carbapenem hydrolysis, which limits test sensitivity and speed, especially for the detection of CPE producing low-activity carbapenem (e.g., OXA-48 variants). Herein, we developed a novel rapid and sensitive CPE detection method (Carba PBP) that could measure substrate (meropenem) consumption based on penicillin-binding protein (PBP)...
January 29, 2024: Journal of Clinical Microbiology