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https://www.readbyqxmd.com/read/28077701/a-comparison-of-eleven-phenotypic-assays-for-the-accurate-detection-of-carbapenemase-producing-enterobacteriaceae
#1
Pranita D Tamma, Belita N A Opene, Andrew Gluck, Krizia K Chambers, Karen C Carroll, Patricia J Simner
BACKGROUND: Early identification of carbapenemase-producing Enterobacteriaceae (CPE) is essential to prevent their dissemination within healthcare settings. Our objective was to evaluate the accuracy of 11 phenotypic assays for the detection of CPE. METHODS: Two collections of carbapenem-resistant Enterobacteriaceae (CRE) isolates were evaluated including 191 retrospective isolates (122 CP-CRE and 69 non-CP isolates) as well as 45 prospective clinical isolates (15 CP-CRE and 30 non-CP-CRE) obtained over a 3-month period...
January 11, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28007530/utilizing-the-carba-np-test-as-an-indicator-of-expression-level-of-carbapenemase-genes-in-enterobacteriaceae
#2
Takaya Segawa, Mari Matsui, Masato Suzuki, Atsuko Tsutsui, Makoto Kuroda, Keigo Shibayama, Satowa Suzuki
The Carba NP test was developed to detect carbapenemase-producing Enterobacteriaceae, and uses imipenem as the reaction substrate. In Japan, IMP-6 metallo-β-lactamase (MBL) producers, which are usually resistant to meropenem but susceptible to imipenem, and IMP-1 MBL producers, which are usually resistant to both carbapenems are prevalent. We performed the Carba NP test with IMP-6 and IMP-1 MBL producers, and both types were detected by the Carba NP test with high sensitivity. All IMP-1 MBL producers were detected by the Carba NP test, but the minimum inhibitory concentrations (MICs) of imipenem varied from 0...
December 19, 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28007519/genetic-characterisation-of-carbapenem-resistant-gram-negative-bacteria-isolated-from-the-university-hospital-mohamed-boudiaf-in-ouargla-southern-algeria
#3
Mounira Yagoubat, Aminata Ould El-Hadj-Khelil, Ali Malki, Sofiane Bakour, Abdelaziz Touati, Jean-Marc Rolain
OBJECTIVES: The aim of this study was to investigate the molecular epidemiology and the genetic support of carbapenem-resistant Enterobacteriaceae and Acinetobacter spp. isolates collected in the University Hospital of Ouargla, southern Algeria. METHODS: A total of 99 Gram-negative bacteria (GNB) were collected from stool samples of colonised patients and from inanimate surfaces in the hospital environment between December 2014 and August 2015. Selected Enterobacteriaceae and Acinetobacter spp...
December 19, 2016: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/27998674/modified-carba-np-test-for-the-detection-of-carbapenemase-production-in-gram-negative-rods-optimized-handling-of-multiple-samples
#4
Eloiza H Campana, Stephanie G Chuster, Isadora R da Silva, Raphael P Paschoal, Raquel R Bonelli, Beatriz M Moreira, Renata C Picão
The modified Carba NP test presented here may be a valuable tool for laboratories interested in investigating a large number of carbapenemase-producing bacteria in a less-costly way. The test was evaluated against 48 carbapenemase-producing and carbapenemase-non-producing gram-negative bacteria. No false-positive results were obtained, but false-negative results were observed with OXA-23- and GES-carbapenemase-producing isolates. Aeromonas sp. are not testable by Modified Carba NP.
December 7, 2016: Brazilian Journal of Microbiology: [publication of the Brazilian Society for Microbiology]
https://www.readbyqxmd.com/read/27927915/comparative-evaluation-of-four-phenotypic-tests-for-the-detection-of-carbapenemase-producing-gram-negative-bacteria
#5
Audrey Noël, Te-Din Huang, Catherine Berhin, Martin Hoebeke, Warda Bouchahrouf, Sami Yunus, Pierre Bogaerts, Youri Glupczynski
Four screening assays aimed for rapid detection of carbapenemase production from Gram-negative bacterial isolates i.e. the Neo-Rapid CARB kit (Rosco Diagnostica A/S), the RAPIDEC CARBA NP test (BioMérieux SA), the β CARBA test (Bio-Rad Laboratories N.V) and a home-made electrochemical assay (BYG Carba test) were evaluated against a panel comprising 328 clinical isolates (Enterobacteriaceae [n= 198], nonfermentative Gram-negative bacilli [n = 130]) with previously characterized resistance mechanisms to carbapenems...
December 7, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27796502/cilastatin-does-not-affect-carba-np-test-performance-for-detection-of-carbapenemase-production-in-enterobacteriaceae
#6
Rainer Hartl, Sarah Widhalm, Heidrun Kerschner, Petra Apfalter, Rainer Gattringer
The Carba NP test is a simple confirmation method for carbapenemase-producing Enterobacteriaceae (CPE) but reagents have to be freshly prepared as imipenem sodium salt is unstable. We evaluated the Carba NP test performance based on a commercially available 10-fold cheaper drug formulation containing cilastatin against 217 CPE and 78 non-CPE isolates with reduced meropenem susceptibility. Specificity and sensitivity were 100 % and 98.6 %, respectively and 3 false negative results of blaVIM-1-producing Proteus mirabilis were reproducible with the RAPIDEC® Carba NP test...
October 28, 2016: Wiener Klinische Wochenschrift
https://www.readbyqxmd.com/read/27795386/evaluation-of-the-carba-np-test-in-oregon-2013
#7
Karim E Morey, Robert Vega, P Maureen Cassidy, Genevieve L Buser, Jaipreet K Rayar, Jeffrey A Myers, Scott J Weissman, Zintars G Beldavs, Christopher D Pfeiffer
Carbapenem-resistant Enterobacteriaceae (CRE) are an urgent public health threat. We evaluated the capacity of the Carba NP test to detect carbapenemase production in 206 isolates: 143 Enterobacteriaceae identified by Oregon's CRE surveillance program in 2013 and 63 known carbapenemase-positive organisms. Overall, test sensitivity and specificity were 89% (59/66 isolates; 95% confidence interval [CI], 81 to 97%) and 100% (140/140 isolates; 95% CI, 98 to 100%), respectively. All KPC, NDM-1, VIM, and IMP producers but no (0/7 isolates) OXA-48-like strains were Carba NP positive prior to a post hoc protocol modification...
January 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/27760718/laboratory-detection-of-intestinal-carriage-of-carbapenemase-producing-enterobacteriaceae-a-comparison-of-algorithms-using-the-carba-np-test
#8
James Knox, Claire Gregory, Louise Prendergast, Chandrika Perera, Jennifer Robson, Lynette Waring
Stool specimens spiked with a panel of 46 carbapenemase-producing Enterobacteriaceae (CPE) and 59 non-carbapenemase producers were used to compare the diagnostic accuracy of 4 testing algorithms for the detection of intestinal carriage of CPE: (1) culture on Brilliance ESBL agar followed by the Carba NP test; (2) Brilliance ESBL followed by the Carba NP test, plus chromID OXA-48 agar with no Carba NP test; (3) chromID CARBA agar followed by the Carba NP test; (4) chromID CARBA followed by the Carba NP test, plus chromID OXA-48 with no Carba NP test...
January 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/27737785/detection-of-carbapenemase-activity-in-enterobacteriaceae-using-lc-ms-ms-in-comparison-with-the-neo-rapid-carb-kit-using-direct-visual-assessment-and-colorimetry
#9
Charlotte A Huber, Hanna E Sidjabat, Hosam M Zowawi, David Kvaskoff, Sarah Reed, John F McNamara, Kate L McCarthy, Patrick Harris, Benjamin Toh, Alexander M Wailan, David L Paterson
It has been described that the sensitivity of the Carba NP test may be low in the case of OXA-48-like carbapenamases and mass spectrometry based methods as well as a colorimetry based method have been described as alternatives. We evaluated 84 Enterobacteriaceae isolates including 31 OXA-48-like producing isolates and 13 isolates that produced either an imipenemase (IMP; n=8), New Delhi metallo-β-lactamase (NDM; n=3), or Klebsiella pneumoniae carbapenemase (KPC; n=2), as well as 40 carbapenemase negative Enterobacteriaceae isolates...
October 11, 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27630840/a-pilot-study-on-carbapenemase-detection-do-we-see-the-same-level-of-agreement-as-with-the-clsi-observations
#10
Agila Kumari Pragasam, Rani Diana Sahni, Shalini Anandan, Archa Sharma, Radha Gopi, Noorjahan Hadibasha, Priya Gunasekaran, Balaji Veeraraghavan
INTRODUCTION: Rapid identification of carbapenemase producing organisms is of great importance for timely detection, treatment and implementation of control measures to prevent the spread. The Modified Hodge Test (MHT) and Carba NP test is recommended by CLSI for the detection of carbapenemases in Enterobacteriaceae. However, MHT may give false positive results or fail to detect metallo β-lactamases (MBLs). In the US, MHT is the most widely used test for detection of carbapenemases and has been found to have a sensitivity and specificity of >90% for bla KPC producers...
July 2016: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/27617554/a-new-case-report-of-urinary-tract-infection-due-to-kpc-3-producing-klebsiella-pneumoniae-st258-in-spain
#11
A Sorlózano-Puerto, D Esteva-Fernández, J Oteo-Iglesias, J M Navarro-Marí, J Gutiérrez-Fernández
OBJECTIVE: We describe the characterization of a new isolated in Spain of Klebsiella pneumoniae ST258 producing KPC-3, carbapenems non-susceptible, recovered from a sample of urine from a patient with urinary tract infection and no history of carbapenems exposure. METHODS: After the isolation, identification of K. pneumoniae was performed by biochemical tests and mass spectrometry. The carbapenems susceptibility testing was performed by microdilution and E-test in cation-adjusted Mueller-Hinton...
September 2016: Archivos Españoles de Urología
https://www.readbyqxmd.com/read/27575912/evaluation-of-the-carbapenem-inactivation-method-for-detection-of-carbapenemase-producing-gram-negative-bacteria-in-comparison-with-the-rapidec-carba-np
#12
Elif Aktaş, Gülşah Malkoçoğlu, Barış Otlu, Ayşegül Çopur Çiçek, Canan Külah, Füsun Cömert, Cemal Sandallı, Nafia Canan Gürsoy, Duygu Erdemir, Mehmet Emin Bulut
Timely detection of carbapenemases by both phenotypic and genotypic methods is essential for developing strategies to control the spread of infections by carbapenem-resistant isolates and related morbidity and mortality. The aim of this study was to compare the performance of a commercial kit, the RAPIDEC(®) CARBA NP, and an in-house technique, the carbapenem inactivation method (CIM), against a panel of 136 carbapenemase- and noncarbapenemase-producing Enterobacteriaceae, Acinetobacter baumannii, and Pseudomonas aeruginosa isolates...
August 30, 2016: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/27546715/identification-of-enterobacteriaceae-and-detection-of-carbapenemases-from-positive-blood-cultures-by-combination-of-maldi-tof-ms-and-carba-np-performed-after-four-hour-subculture-in-mueller-hinton
#13
Javier Fernández, Carlos Rodríguez-Lucas, Jonathan Fernández-Suárez, Fernando Vazquez, M Rosario Rodicio
A new protocol for Enterobacteriaceae identification and detection of carbapenemase-producing isolates from blood cultures by combining MALDI-TOF MS and the Carba NP test has been evaluated. Bacterial identification was correct in 129 of the 130 isolates tested while the Carba NP detected 28 out of the 29 carbapenemase producers.
October 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27443547/carbapenem-inactivation-a-very-affordable-and-highly-specific-method-for-phenotypic-detection-of-carbapenemase-producing-pseudomonas-aeruginosa-isolates-compared-with-other-methods
#14
Mohammad Taghi Akhi, Younes Khalili, Reza Ghottaslou, Hossein Samadi Kafil, Saber Yousefi, Behroz Nagili, Hamid Reza Goli
This investigation was undertaken to compare phenotypic and molecular methods for detection of carbapenemase-producing Pseudomonas aeruginosa. A total of 245 non-duplicated isolates of P. aeruginosa were collected from hospitalized patients. Disc diffusion method was used to identify carbapenem-resistant bacteria. Three phenotypic methods, including Modified Hodge Test (MHT), Modified Carba NP (MCNP) test and Carbapenem Inactivation Method (CIM) were used for investigation of carbapenemase production. In addition, polymerase chain reaction (PCR) was used to detect carbapenemase encoding genes...
July 22, 2016: Journal of Chemotherapy
https://www.readbyqxmd.com/read/27329263/comparison-of-the-modified-hodge-test-carba-np-test-and-carbapenem-inactivation-method-as-screening-methods-for-carbapenemase-producing-enterobacteriaceae
#15
Kageto Yamada, Machiko Kashiwa, Katsumi Arai, Noriyuki Nagano, Ryoichi Saito
We compared three screening methods for carbapenemase-producing Enterobacteriaceae. While the Modified-Hodge test and Carba NP test produced false-negative results for OXA-48-like and mucoid NDM producers, the carbapenem inactivation method (CIM) showed positive results for these isolates. Although the CIM required cultivation time, it is well suited for general clinical laboratories.
September 2016: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/27263012/modification-and-evaluation-of-the-carba-np-test-by-use-of-paper-strip-for-simple-and-rapid-detection-of-carbapenemase-producing-enterobacteriaceae
#16
Arpasiri Srisrattakarn, Aroonlug Lulitanond, Chotechana Wilailuckana, Nicha Charoensri, Lumyai Wonglakorn, Sirikan Piyapatthanakul, Ampai Supajeen, Aroonwadee Chanawong
Carbapenemase-producing Enterobacteriaceae (CPE) isolates have now emerged worldwide. We therefore modified the phenotypic Carba NP test by use of a filter paper strip for easily and rapidly identifying CPE in routine laboratory. A collection of 56 CPE and carbapenemase-producing Pseudomonas spp. isolates (including 28 NDM-1, 11 IMP-14a, 1 IMP-1, 1 IMP-4, 1 IMP-9, 1 IMP-15, 4 VIM-2, 1 VIM-1, 1 IMP-14a with VIM-2, 3 OXA-48, 3 OXA-181 and 1 KPC-2 producers) and 41 non-CPE isolates (including 19 ESBL, 7 pAmpC, 3 AmpC, 9 ESBL with pAmpC and 3 non-ESBL & non-AmpC producers) as confirmed by the PCR methods were tested by the paper strip method using pharmaceutical imipenem/cilastatin as a substrate...
July 2016: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27060003/overexpression-of-mexab-oprm-efflux-pump-in-carbapenem-resistant-pseudomonas-aeruginosa
#17
Ya-Ping Pan, Yuan-Hong Xu, Zhong-Xin Wang, Ya-Ping Fang, Ji-Lu Shen
Efflux pump systems are one of the most important mechanisms conferring multidrug resistance in Pseudomonas aeruginosa. MexAB-OprM efflux pump is one of the largest multi-drug resistant efflux pumps with high-level expression, which is controlled by regulatory genes mexR, nalC, and nalD. This study investigated the role of efflux pump MexAB-OprM in 75 strains of carbapenem-resistant P. aeruginosa and evaluated the influence of point mutation of the regulatory genes. The minimum inhibitory concentrations of imipenem and meropenem, with or without MC207110, an efflux pump inhibitor, were determined by agar dilution method to select the positive strains for an overexpressed active efflux pump...
August 2016: Archives of Microbiology
https://www.readbyqxmd.com/read/27058324/-comparison-of-the-modified-hodge-test-and-the-carba-np-test-for-detection-of-carbapenemases-in-enterobacteriaceae-isolates
#18
COMPARATIVE STUDY
Gülçin Bayramoğlu, Gülşen Uluçam, Çiğdem Gençoğlu Özgür, Ali Osman Kılıç, Faruk Aydın
A rapid, practical, and accurate identification of carbapenemase-producing Enterobacteriaceae isolates is crucial for the implementation of appropriate infection control measures and proper treatment of the infections. For this purpose, a large number of phenotypic test methods have been developed, although none has 100% sensitivity and specificity. Variations in sensitivity and specificity of these tests based on the type of beta-lactamase enzymes carried by that isolates might result in differences between regions and countries...
January 2016: Mikrobiyoloji Bülteni
https://www.readbyqxmd.com/read/27037274/evaluation-of-two-commercial-carbapenemase-gene-assays-the-rapidec-carba-np-test-and-the-in-house-rapid-carba-np-test-on-bacterial-cultures
#19
LETTER
Monica Österblad, Laura Lindholm, Jari Jalava
No abstract text is available yet for this article.
July 2016: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/26971634/genotypic-and-phenotypic-identification-of-aeromonas-species-and-cpha-mediated-carbapenem-resistance-in-queensland-australia
#20
Holly A Sinclair, Claire Heney, Hanna E Sidjabat, Narelle M George, Haakon Bergh, Snehal N Anuj, Graeme R Nimmo, David L Paterson
Infection caused by Aeromonas spp. ranges from superficial wound infection to life-threatening septicemia. Carbapenem resistance due to metallo-beta-lactamase, CphA encoded by the cphA gene, is a significant problem. This study defines Aeromonas spp. causing clinical disease in Queensland, Australia. Phenotypic tests for carbapenemase detection were assessed. One hundred Aeromonas isolates from blood (22), wound (46), sterile sites (11), stool (18), eye (2), and sputum (1) were characterized by rpoB and gyrB sequencing...
May 2016: Diagnostic Microbiology and Infectious Disease
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