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https://www.readbyqxmd.com/read/28859446/oxa-427-a-new-plasmid-borne-carbapenem-hydrolysing-class-d-%C3%AE-lactamase-in-enterobacteriaceae
#1
Pierre Bogaerts, Thierry Naas, Veroniek Saegeman, Remy A Bonnin, Annette Schuermans, Stéphanie Evrard, Warda Bouchahrouf, Thomas Jove, Didier Tande, Xavier de Bolle, Te-Din Huang, Laurent Dortet, Youri Glupczynski
Objectives: To describe a novel plasmid-borne class D carbapenemase (CHDL) named OXA-427 identified in several Enterobacteriaceae clinical isolates from nine patients in one Belgian hospital. Methods: OXA-427-producing isolates were analysed by an electrochemical imipenem hydrolysis method (BYG Carba test), Carba NP test, conventional phenotypic assays and by molecular methods (PCR, whole sequencing of the OXA-427-encoding plasmid and cloning). The antimicrobial resistance profile of OXA-427 was analysed by expression of the cloned gene in Escherichia coli DH10B and J53...
September 1, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28820383/carbapenemase-producing-enterobacteriaceae-and-nonfermentative-bacteria-the-philippines-2013-2016
#2
John Mark Velasco, Maria Theresa Valderama, Trent Peacock, Nirdnoy Warawadee, Kathyleen Nogrado, Fatima Claire Navarro, Domingo Chua, Srijan Apichai, Ruekit Sirigade, Louis R Macareo, Brett Swierczewski
During 2013-2016, we isolated blaNDM- and blaVIM-harboring Enterobacteriaceae and nonfermentative bacteria from patients in the Philippines. Of 130 carbapenem-resistant isolates tested, 45 were Carba NP-positive; 43 harbored blaNDM, and 2 harbored blaVIM. Multidrug-resistant microbial pathogen surveillance and antimicrobial drug stewardship are needed to prevent further spread of New Delhi metallo-β-lactamase variants.
September 2017: Emerging Infectious Diseases
https://www.readbyqxmd.com/read/28744664/evaluation-of-the-carba-np-test-for-carbapenemase-detection-in-enterobacteriaceae-pseudomonas-spp-and-acinetobacter-spp-and-its-practical-use-in-the-routine-work-of-a-national-reference-laboratory-for-susceptibility-testing
#3
E Literacka, M Herda, A Baraniak, D Żabicka, W Hryniewicz, A Skoczyńska, M Gniadkowski
The aim of this study was to evaluate the Carba NP test (and CarbAcineto) for the detection of carbapenemases in Enterobacteriaceae, Pseudomonas spp. and Acinetobacter spp., and to assess its usefulness in the routine work of the National Reference Centre for Susceptibility Testing (NRCST) in Poland. The evaluation of the Carba NP/CarbAcineto tests was carried out on a group of 81 Enterobacteriaceae, Pseudomonas spp. and Acinetobacter spp. isolates producing KPC-, NDM-, VIM-, IMP- or OXA-48, -23, -24/40, -58-type carbapenemases, and on 26 carbapenemase-negative strains cultivated on a broad panel of microbiological media...
July 25, 2017: European Journal of Clinical Microbiology & Infectious Diseases
https://www.readbyqxmd.com/read/28701421/carbapenemase-detection-amongst-carbapenem-resistant-glucose-nonfermenting-gram-negative-bacilli
#4
Patricia J Simner, Belita N A Opene, Krizia K Chambers, Matthew E Naumann, Karen C Carroll, Pranita D Tamma
Background: Accurate detection of carbapenemase-producing glucose nonfermenting gram-negative bacilli (CPNF), including Pseudomonas aeruginosa and Acinetobacter baumannii, is necessary to prevent their dissemination within health care settings. We performed a methods comparison study of 11 phenotypic carbapenemase-detection assays to evaluate their accuracy for the detection of CPNFs.Methods: 96 carbapenem-resistant glucose nonfermenting isolates were included, of which 29% produced carbapenemases. All CPNFs were molecularly characterized to identify β-lactamase genes...
July 12, 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28691891/incidence-of-oxa-23-and-oxa-58-carbapenemases-coexpressed-in-clinical-isolates-of-acinetobacter-baumannii-in-tunisia
#5
Najla Mathlouthi, Yomna Ben Lamine, Rania Somai, Sophia Bouhalila-Besbes, Sofiane Bakour, Jean-Marc Rolain, Chedly Chouchani
Acinetobacter baumannii is an important opportunistic and multidrug-resistant pathogen responsible for nosocomial infections in health facilities. The aim of this study was to characterize the molecular mechanisms of carbapenem resistance in A. baumannii isolates isolated from Mohamed Kassab Orthopedic Institute in Tunis, Tunisia. Twenty-five imipenem-resistant A. baumannii clinical isolates collected between 2013 and 2016 were identified using API 20NE and were confirmed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS)...
July 10, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28674064/oxa-244-producing-escherichia-coli-isolates-a-challenge-for-clinical-microbiology-laboratories
#6
Yannick Hoyos-Mallecot, Thierry Naas, Rémy A Bonnin, Rafael Patino, Philippe Glaser, Nicolas Fortineau, Laurent Dortet
OXA-244 is a single-point-mutant derivative of OXA-48 displaying reduced carbapenemase activity. Here, we report the microbiological features of seven OXA-244-producing Escherichia coli isolates. Only one isolate grew on ChromID Carba Smart medium (bioMérieux), but six of the seven isolates grew on ChromID extended-spectrum-β-lactamase (ESBL) medium (bioMérieux), as they coproduced an ESBL and/or a plasmid-encoded cephalosporinase. The production of a carbapenemase was detected in 57.1%, 71.4%, 71.4%, and 100% of the E...
September 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28613981/detection-of-new-delhi-metallo-%C3%AE-lactamase-variants-ndm-4-ndm-5-and-ndm-7-in-enterobacter-aerogenes-isolated-from-a-neonatal-intensive-care-unit-of-a-north-india-hospital-a-first-report
#7
Nayeem Ahmad, Syed Manazir Ali, Asad U Khan
A total 402 Enterobacteriaceae isolates were recovered from blood and rectal swabs of 1,000 infants admitted to the Neonatal Intensive Care Unit (NICU) of the Jawaharlal Medical College and Hospital Aligarh, India. Carbapenamase producers were determined by Carba NP phenotype biochemical assay. Out of 402 isolates, it was the first time three of the isolates were identified as Enterobacter aerogenes carrying blaNDM-4, blaNDM-5, and blaNDM-7 genes. These genes were identified by polymerase chain reaction (PCR) and sequence analysis...
June 14, 2017: Microbial Drug Resistance: MDR: Mechanisms, Epidemiology, and Disease
https://www.readbyqxmd.com/read/28605515/fri-2-carbapenemase-producing-enterobacter-cloacae-complex-in-the-uk
#8
Danièle Meunier, Jacqueline Findlay, Michel Doumith, Daniel Godoy, Claire Perry, Rachel Pike, Firza Gronthoud, Theresa Shryane, Laurent Poirel, William Welfare, Neil Woodford, Katie L Hopkins
Objectives: Detection of rarer carbapenemases is challenging, as it requires molecular assays with comprehensive coverage or the use of phenotypic methods for the detection of carbapenemase activity. We describe a new class A carbapenemase, FRI-2, in an Enterobacter cloacae complex isolate following implementation of an in-house multiplex PCR for the detection of 'rare' class A carbapenemases. Methods: MICs were determined by agar dilution. A carbapenem-resistant E...
September 1, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28535303/a-novel-goldnano-carb-test-for-rapid-phenotypic-detection-of-carbapenemases-particularly-oxa-type-in-enterobacteriaceae-pseudomonas-aeruginosa-and-acinetobacter-spp
#9
Arpasiri Srisrattakarn, Aroonlug Lulitanond, Chotechana Wilailuckana, Nicha Charoensri, Jureerut Daduang, Aroonwadee Chanawong
Objectives: To develop a simple gold nanoparticle (AuNP)-based colorimetric test, GoldNano Carb (GoldC), for detecting carbapenemase production in Gram-negative bacteria, compared with updated Carba NP (CNP) and CarbAcineto NP (CAcNP) tests by using PCR methods as gold standard. Methods: Ninety-nine carbapenemase-producing Enterobacteriaceae (CPE), Pseudomonas spp. and Acinetobacter spp. isolates and 89 non-CPE isolates were tested by the GoldC and CNP. Additionally, the CAcNP was performed in the Acinetobacter spp...
September 1, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28533001/evaluation-of-the-rapidec%C3%A2-carba-np-and-%C3%AE-carba%C3%A2-tests-for-rapid-detection-of-carbapenemase-producing-enterobacteriaceae
#10
Stefano Mancini, Nicolas Kieffer, Laurent Poirel, Patrice Nordmann
The analytical performances of the RAPIDEC CARBA NP® (bioMérieux) and the β-CARBA® (Bio-Rad) tests were evaluated for the rapid detection of any known type of carbapenemases in Enterobacteriaceae. An international collection of 149 enterobacterial isolates comprising 111 Carbapenemase-Producing Enterobacteriaceae (CPE) and 38 non-carbapenemase producers was used. CPE included 32 carbapenemase producers of class A (18 KPC-2, 1 FRI-1, 5 SME and 8 IMI), 33 of class B (13 NDM, 11 VIM and 9 IMP) and 46 of class D (15 OXA-48, 14 OXA-181, 10 OXA-204, 3 OXA-232 and 4 OXA-162)...
May 13, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28502395/multicenter-evaluation-of-the-rapidec%C3%A2-carba-np-test-for-rapid-screening-of-carbapenemase-producing-enterobacteriaceae-and-gram-negative-nonfermenters-from-clinical-specimens
#11
Marco Coppi, Alberto Antonelli, Tommaso Giani, Teresa Spanu, Flora Marzia Liotti, Carla Fontana, Walter Mirandola, Raffaele Gargiulo, Agostino Barozzi, Carola Mauri, Luigi Principe, Gian Maria Rossolini
The rapid diagnosis of carbapenemase-producing (CP) bacteria is essential for the management of therapy and infection control. In this study, RAPIDEC® CARBA NP (RCNP) was evaluated for the rapid screening of CP Enterobacteriaceae, Acinetobacter baumannii complex, and Pseudomonas aeruginosa from clinical specimens collected at five Italian hospitals. Firstly, each site tested 20 well-characterized strains in a blinded fashion. Secondly, each center prospectively tested 25 isolates from blood cultures processed with a rapid workflow (6h after subculture) and 25 isolates from other specimens processed after an overnight culture...
April 27, 2017: Diagnostic Microbiology and Infectious Disease
https://www.readbyqxmd.com/read/28486169/characterization-of-oxa-48-like-producing-enterobacteriaceae-isolated-from-river-water-in-algeria
#12
Rima Tafoukt, Abdelaziz Touati, Thongpan Leangapichart, Sofiane Bakour, Jean-Marc Rolain
The spread of carbapenemase-producing Enterobacteriaceae (CPE) is a significant problem for healthcare worldwide. The prevalence of carbapenem-resistant Enterobacteriaceae (CPE) in water environments in Algeria are unknown. The aim of this study was to screen for the presence of CPE isolates in the Soummam River in Bejaia, Algeria. Isolates of Enterobacteriaceae recovered from twelve samples of river water and showing reduced susceptibility to carbapenems were included in this study. The isolates were identified by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)...
April 30, 2017: Water Research
https://www.readbyqxmd.com/read/28445009/evaluation-of-six-phenotypic-methods-for-the-detection-of-carbapenemases-in-gram-negative-bacteria-with-characterized-resistance-mechanisms
#13
Kunling Sun, Xiuyu Xu, Jinrong Yan, Liping Zhang
BACKGROUND: We compared the performance of the modified Hodge test (MHT), Triton Hodge test (THT), Carba NP test (CNPt), simplified Carba NP test (CNPt-direct), blue-Carba NP test (BCT), and carbapenem inactivation method (CIM) for rapid and accurate carbapenemase detection. METHODS: The methods were evaluated by using 256 gram-negative isolates, including 197 Enterobacteriaceae (79 Enterobacter spp., 74 Klebsiella spp., 33 Escherichia coli, 10 Citrobacter spp., and 1 Serratia marcescens), 51 Acinetobacter baumannii, and 8 Pseudomonas aeruginosa strains...
July 2017: Annals of Laboratory Medicine
https://www.readbyqxmd.com/read/28404676/multicenter-performance-assessment-of-carba-np-test
#14
Scott A Cunningham, Brandi Limbago, Maria Traczewski, Karen Anderson, Meredith Hackel, Janet Hindler, Dan Sahm, Efe Alyanak, Adrian Lawsin, Christopher A Gulvik, Tom J B de Man, Jayawant N Mandrekar, Audrey N Schuetz, Stephen Jenkins, Romney Humphries, Elizabeth Palavecino, Shawn Vasoo, Robin Patel
Eighty Gram-negative bacilli (54 Enterobacteriaceae and 26 nonfermenting Gram-negative bacilli) obtained from multiple institutions in the United States were distributed in a blinded manner to seven testing laboratories to compare their performance of a test for detection of carbapenemase production, the Carba NP test. The Carba NP test was performed by all laboratories, following the Clinical and Laboratory Standards Institute (CLSI) procedure. Site-versus-site comparisons demonstrated a high level of consistency for the Carba NP assay, with just 3/21 site comparisons yielding a difference in sensitivity (P < 0...
June 2017: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/28384185/evaluation-of-a-modified-meropenem-hydrolysis-assay-on-a-large-cohort-of-kpc-and-vim-carbapenemase-producing-enterobacteriaceae
#15
Adriana Calderaro, Mirko Buttrini, Maddalena Piergianni, Sara Montecchini, Monica Martinelli, Silvia Covan, Giovanna Piccolo, Maria Cristina Medici, Maria Cristina Arcangeletti, Carlo Chezzi, Flora De Conto
Carbapenem-resistant Enterobacteriaceae (CRE) have spread globally and represent a serious and growing threat to public health. The introduction of rapid and sensitive methods for the detection of carbapenemase-producing bacteria is of increasing importance. The carbapenemase production can be detected using non-molecular methods (such as the modified Hodge test, the synergy test, the Carba NP test and the antibiotic hydrolysis assays) and DNA-based methods. In this study, we propose a modified version of a previously described meropenem hydrolysis assay (MHA) by MALDI-TOF MS for the phenotypic detection in 2h of carbapenemase-producing Enterobacteriaceae...
2017: PloS One
https://www.readbyqxmd.com/read/28367024/carba-np-as-a-simpler-rapid-cost-effective-and-a-more-sensitive-alternative-to-other-phenotypic-tests-for-detection-of-carbapenem-resistance-in-routine-diagnostic-laboratories
#16
Shivani Shinde, Rajarshi Gupta, Shweta S Raut, Gita Nataraj, Preeti R Mehta
PURPOSE: Resistance to carbapenems due to carbapenemases has been increasingly noticed in Enterobacteriaceae. Clinical and Laboratory Standards Institute (CLSI) has recommended the latest Carba NP (CNP) test as a confirmatory test for carbapenemase production in Enterobacteriaceae. Low sensitivity of disk diffusion (DD) and modified Hodge test (MHT) may result in missing out of resistant strains which can adversely affect clinical management. The present study compares three phenotypic tests - CNP test, DD, and MHT for detection of carbapenemase production...
April 2017: Journal of Laboratory Physicians
https://www.readbyqxmd.com/read/28333363/evaluation-of-the-%C3%AE-carba%C3%A2-test-a-colorimetric-test-for-the-rapid-detection-of-carbapenemase-activity-in-gram-negative-bacilli
#17
Sandrine Bernabeu, Laurent Dortet, Thierry Naas
Objectives: There is an urgent need for accurate and fast diagnostic tests to identify carbapenemase-producing bacteria. Here, we have evaluated a novel colorimetric test (the β-CARBA™ test; Bio-Rad) to detect carbapenemase-producing Gram-negative bacilli from cultured colonies. Methods: The performance of the β-CARBA™ test was compared with that of the Carba NP test (or the CarbAcineto NP test) and RAPIDEC ® CARBA NP (bioMérieux) using a collection of 290 isolates with characterized β-lactamase content...
June 1, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28303831/strengths-and-limitations-of-various-screening-methods-for-carbapenem-resistant-enterobacteriaceae-including-new-method-recommended-by-clinical-and-laboratory-standards-institute-2017-a-tertiary-care-experience
#18
Agila Kumari Pragasam, Balaji Veeraraghavan, Yamuna Devi Bakthavatchalam, Radha Gopi, Raziya Fathima Aslam
Carbapenemase-mediated carbapenem resistance is a major concern across the world. Rapid detection of carbapenemase-producing organisms is of great importance in clinical settings. However, it is essential to have a test with good sensitivity and specificity. The aim of the study was to compare the performance of RAPIDEC® CARBA NP and modified carbapenem inactivation method (mCIM) recommended by Clinical and Laboratory Standards Institute guideline 2017. A total of ninety carbapenem resistant Escherichia coli and Klebsiella pneumoniae have been tested...
January 2017: Indian Journal of Medical Microbiology
https://www.readbyqxmd.com/read/28286140/detection-of-carbapenemase-producers-evaluating-the-performance-of-the-carbapenem-inactivation-method-and-carba-np-test-versus-multiplex-pcr
#19
Lamiaa A Madkour, May S Soliman, Dina M Hassan, Noha S Soliman, Yasmin A ElMahdy
OBJECTIVES: With a worrisome surge of carbapenem-resistant bacterial isolates, the diagnostic arsenal has become in dire need of affordable and timely assays to detect the rapidly transmissible carbapenemases. Employing multiplex PCR as a reference method, the purpose of the present study was to compare the performance of the carbapenem inactivation method (CIM) and the Carba NP test in the detection of carbapenemase-producers. METHODS: A panel of 203 Gram-negative bacterial isolates screened for carbapenem resistance were subjected to the CIM and Carba NP test...
June 2017: Journal of Global Antimicrobial Resistance
https://www.readbyqxmd.com/read/28223374/enterobacter-cloacae-complex-isolates-harboring-blanmc-a-or-blaimi-type-class-a-carbapenemase-genes-on-novel-chromosomal-integrative-elements-and-plasmids
#20
David A Boyd, Laura F Mataseje, Ross Davidson, Johannes A Delport, Jeff Fuller, Linda Hoang, Brigitte Lefebvre, Paul N Levett, Diane L Roscoe, Barbara M Willey, Michael R Mulvey
Carbapenem-resistant Enterobacter cloacae complex isolates submitted to a reference laboratory from 2010 to 2015 were screened by PCR for seven common carbapenemase gene groups, namely, KPC, NDM, OXA-48, VIM, IMP, GES, and NMC-A/IMI. Nineteen of the submitted isolates (1.7%) were found to harbor Ambler class A blaNMC-A or blaIMI-type carbapenemases. All 19 isolates were resistant to at least one carbapenem but susceptible to aminoglycosides, trimethoprim-sulfamethoxazole, tigecycline, and ciprofloxacin. Most isolates (17/19) gave positive results with the Carba-NP test for phenotypic carbapenemase detection...
May 2017: Antimicrobial Agents and Chemotherapy
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