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lipidomic biomarkers

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https://www.readbyqxmd.com/read/28502800/microrna-211-regulates-oxidative-phosphorylation-and-energy-metabolism-in-human-vitiligo
#1
Anupama Sahoo, Bongyong Lee, Katia Boniface, Julien Seneschal, Sanjaya K Sahoo, Tatsuya Seki, Chunyan Wang, Soumen Das, Xianlin Han, Michael Steppie, Sudipta Seal, Alain Taieb, Ranjan J Perera
Vitiligo is a common, chronic skin disorder characterized by loss of epidermal melanocytes and progressive depigmentation. Vitiligo has complex immune, genetic, environmental, and biochemical etiology, but the exact molecular mechanisms of vitiligo development and progression, particularly those related to metabolic control, are poorly understood. Here we characterized the human vitiligo cell line PIG3V and the normal human melanocytes, HEM-l by RNA-sequencing (RNA-seq), targeted metabolomics, and shotgun lipidomics...
May 11, 2017: Journal of Investigative Dermatology
https://www.readbyqxmd.com/read/28495569/journal-of-pharmaceutical-sciences-notes-plasma-lipidomics-of-healthy-japanese-adults-reveals-gender-and-age-related-differences
#2
Keiko Maekawa, Kazuo Okemoto, Masaki Ishikawa, Rieko Tanaka, Yuji Kumagai, Yoshiro Saito
Lipid metabolites in the blood are expected to be biomarker candidates to reflect disease states and responses to therapeutic drugs. However, their profiles are influenced by subject background, which may lead to confounding results. This study aimed to evaluate whether age and gender affect lipid metabolite levels in the plasma of healthy Japanese adults. Comprehensive lipidomic analysis was performed using liquid chromatography-mass spectrometry for overnight-fasted volunteers consisting of four groups of 15 subjects each: young males (25-35 years old), elderly males (55-64 years old), young females (25-35 years old), and elderly females (55-65 years old)...
May 8, 2017: Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/28475424/metabolic-dysregulation-after-neutron-exposures-expected-from-an-improvised-nuclear-device
#3
Evagelia C Laiakis, Yi-Wen Wang, Erik F Young, Andrew D Harken, Yanping Xu, Lubomir Smilenov, Guy Y Garty, David J Brenner, Albert J Fornace
The increased threat of terrorism across the globe has raised fears that certain groups will acquire and use radioactive materials to inflict maximum damage. In the event that an improvised nuclear device (IND) is detonated, a potentially large population of victims will require assessment for radiation exposure. While photons will contribute to a major portion of the dose, neutrons may be responsible for the severity of the biologic effects and cellular responses. We investigated differences in response between these two radiation types by using metabolomics and lipidomics to identify biomarkers in urine and blood of wild-type C57BL/6 male mice...
May 5, 2017: Radiation Research
https://www.readbyqxmd.com/read/28474568/lipid-biomarkers-in-alzheimer-s-disease
#4
Amira Zarrouk, Meryam Debbabi, Maryem Bezine, El Mostafa Karym, Asmaa Badreddine, Olivier Rouaud, Thibault Moreau, Mustapha Cherkaoui-Malki, Mohamed El Ayeb, Boubker Nasser, Mohamed Hammami, Gérard Lizard
Alzheimer's disease (AD) is the most frequent form of dementia. It is characterized by three successive stages (preclinical, mild cognitive impairment (MCI), and dementia), which are determined with a panel of complementary tests including behavioral and biochemical tests and imaging methods. Currently accepted biomarkers of AD include levels of brain chemicals related to amyloid or tau, and imaging-derived estimates of the size and metabolic activity of specific brain regions. At the moment, the pathogenesis of AD is still poorly understood but some studies support a link between lipid metabolism disorders and AD...
May 4, 2017: Current Alzheimer Research
https://www.readbyqxmd.com/read/28456385/changes-in-red-blood-cell-membrane-lipid-composition-a-new-perspective-into-the-pathogenesis-of-pkan
#5
Manar Aoun, Paola Antonia Corsetto, Guillaume Nugue, Gigliola Montorfano, Emilio Ciusani, David Crouzier, Penelope Hogarth, Allison Gregory, Susan Hayflick, Giovanna Zorzi, Angela Maria Rizzo, Valeria Tiranti
Pantothenate Kinase-Associated Neurodegeneration (PKAN) is a form of Neurodegeneration with Brain Iron Accumulation (NBIA) associated with mutations in the pantothenate kinase 2 gene (PANK2). The PANK2 catalyzes the first step of coenzyme A (CoA) biosynthesis, a pathway producing an essential cofactor that plays a key role in energy and lipid metabolism. The majority of PANK2 mutations reduces or abolishes the activity of the enzyme. In around 10% of cases with PKAN, the presence of deformed red blood cells with thorny protrusions in the circulation has been detected...
April 18, 2017: Molecular Genetics and Metabolism
https://www.readbyqxmd.com/read/28453542/phospholipid-profiling-of-plasma-from-gw-veterans-and-rodent-models-to-identify-potential-biomarkers-of-gulf-war-illness
#6
Tanja Emmerich, Zuchra Zakirova, Nancy Klimas, Kimberly Sullivan, Ashok K Shetty, James E Evans, Ghania Ait-Ghezala, Gary S Laco, Bharathi Hattiangady, Geetha A Shetty, Michael Mullan, Gogce Crynen, Laila Abdullah, Fiona Crawford
Gulf War Illness (GWI), which affects at least one fourth of the 700,000 veterans deployed to the Gulf War (GW), is characterized by persistent and heterogeneous symptoms, including pain, fatigue and cognitive problems. As a consequence, this illness remains difficult to diagnose. Rodent models have been shown to exhibit different symptomatic features of GWI following exposure to particular GW agents (e.g. pyridostigmine bromide, permethrin and DEET) and/or stress. Preclinical analyses have shown the activation of microglia and astroglia as a pathological hallmark in these mouse and rat models...
2017: PloS One
https://www.readbyqxmd.com/read/28419726/chemotaxonomic-characterization-of-the-thaumarchaeal-lipidome
#7
Felix J Elling, Martin Könneke, Graeme W Nicol, Michaela Stieglmeier, Barbara Bayer, Eva Spieck, José R de la Torre, Kevin W Becker, Michael Thomm, James I Prosser, Gerhard J Herndl, Christa Schleper, Kai-Uwe Hinrichs
Thaumarchaeota are globally distributed and abundant microorganisms occurring in diverse habitats and thus represent a major source of archaeal lipids. The scope of lipids as taxonomic markers in microbial ecological studies is limited by the scarcity of comparative data on the membrane lipid composition of cultivated representatives, including the phylum Thaumarchaeota. Here, we comprehensively describe the core and intact polar lipid (IPL) inventory of ten ammonia-oxidizing thaumarchaeal cultures representing all four characterized phylogenetic clades...
April 17, 2017: Environmental Microbiology
https://www.readbyqxmd.com/read/28405621/lipidfinder-a-computational-workflow-for-discovery-of-lipids-identifies-eicosanoid-phosphoinositides-in-platelets
#8
Anne O'Connor, Christopher J Brasher, David A Slatter, Sven W Meckelmann, Jade I Hawksworth, Stuart M Allen, Valerie B O'Donnell
Accurate and high-quality curation of lipidomic datasets generated from plasma, cells, or tissues is becoming essential for cell biology investigations and biomarker discovery for personalized medicine. However, a major challenge lies in removing artifacts otherwise mistakenly interpreted as real lipids from large mass spectrometry files (>60 K features), while retaining genuine ions in the dataset. This requires powerful informatics tools; however, available workflows have not been tailored specifically for lipidomics, particularly discovery research...
April 6, 2017: JCI Insight
https://www.readbyqxmd.com/read/28391860/lipidomic-analysis-for-carbonyl-species-derived-from-fish-oil-using-liquid-chromatography-tandem-mass-spectrometry
#9
Joon Hyuk Suh, Yue S Niu, Wei-Lun Hung, Chi-Tang Ho, Yu Wang
Lipid peroxidation gives rise to carbonyl species, some of which are reactive and play a role in the pathogenesis of numerous human diseases. Oils are ubiquitous sources that can be easily oxidized to generate these compounds under oxidative stress. In this present work, we developed a targeted lipidomic method for the simultaneous determination of thirty-five aldehydes and ketones derived from fish oil, the omega-3 fatty acid-rich source, by using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The analytes include highly toxic reactive carbonyl species (RCS) such as acrolein, crotonaldehyde, trans-4-hydroxy-2-hexenal (HHE), trans-4-hydroxy-2-nonenal (HNE), trans-4-oxo-2-nonenal (ONE), glyoxal and methylglyoxal, all of which are promising biomarkers of lipid peroxidation...
June 1, 2017: Talanta
https://www.readbyqxmd.com/read/28372724/simultaneous-metabolomics-and-lipidomics-analysis-based-on-novel-heart-cutting-two-dimensional-liquid-chromatography-mass-spectrometry
#10
Shuangyuan Wang, Lina Zhou, Zhichao Wang, Xianzhe Shi, Guowang Xu
Increasing metabolite coverage by combining data from different platforms or methods can improve understanding of related metabolic mechanisms and the identification of biomarkers. However, no one method can obtain metabolomic and lipidomic information in a single analysis. In this work, aiming at collecting comprehensive information on metabolome and lipidome in a single analytical run, we developed an on-line heart-cutting two-dimensional liquid chromatography-mass spectrometry (2D-LC-MS) method. Complex metabolites from biological samples are divided into two fractions by using a precolumn...
May 8, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/28344632/dynamic-and-temporal-assessment-of-human-dried-blood-spot-ms-ms-all-shotgun-lipidomics-analysis
#11
Fei Gao, Justice McDaniel, Emily Y Chen, Hannah E Rockwell, Jeremy Drolet, Vivek K Vishnudas, Vladimir Tolstikov, Rangaprasad Sarangarajan, Niven R Narain, Michael A Kiebish
BACKGROUND: Real-time and dynamic assessment of an individual's lipid homeostatic state in blood is complicated due to the need to collect samples in a clinical environment. In the context of precision medicine and population health, tools that facilitate sample collection and empower the individual to participate in the process are necessary to complement advanced bioanalytical analysis. The dried blood spot (DBS) methodology via finger prick or heel prick is a minimally invasive sample collection method that allows the relative ease and low cost of sample collection as well as transport...
2017: Nutrition & Metabolism
https://www.readbyqxmd.com/read/28339984/the-link-between-phenotype-and-fatty-acid-metabolism-in-advanced-chronic-kidney-disease
#12
Dan-Qian Chen, Hua Chen, Lin Chen, Nosratola D Vaziri, Ming Wang, Xiang-Ri Li, Ying-Yong Zhao
Background.: The kidney plays a central role in elimination of metabolic waste products and regulation of low-molecular weight metabolites via glomerular filtration, tubular secretion and reabsorption. Disruption of these processes results in profound changes in the biochemical milieu of the body fluids, which contribute to complications of chronic kidney disease (CKD) by inducing cytotoxicity and inflammation. Insight into the changes of the composition of metabolites and dysregulation of target genes and proteins enhances the understanding of the pathophysiology of CKD and its complications, and the development of novel therapeutic strategies...
March 1, 2017: Nephrology, Dialysis, Transplantation
https://www.readbyqxmd.com/read/28333036/apoe-%C3%AE%C2%B54-specific-imbalance-of-arachidonic-acid-and-docosahexaenoic-acid-in-serum-phospholipids-identifies-individuals-with-preclinical-mild-cognitive-impairment-alzheimer-s-disease
#13
Laila Abdullah, James E Evans, Tanja Emmerich, Gogce Crynen, Ben Shackleton, Andrew P Keegan, Cheryl Luis, Leon Tai, Mary J LaDu, Michael Mullan, Fiona Crawford, Corbin Bachmeier
This study was designed to explore the influence of apolipoprotein E (APOE) on blood phospholipids (PL) in predicting preclinical Alzheimer's disease (AD). Lipidomic analyses were also performed on blood from an AD mouse model expressing human APOE isoforms (EFAD) and five AD mutations and from 195 cognitively normal participants, 23 of who converted to mild cognitive impairment (MCI)/AD within 3 years. APOE ε4-carriers converting to MCI/AD had high arachidonic acid (AA)/docosahexaenoic acid (DHA) ratios in PL compared to cognitively normal ε4 and non-ε4 carriers...
March 23, 2017: Aging
https://www.readbyqxmd.com/read/28317846/serum-and-synovial-fluid-lipidomic-profiles-predict-obesity-associated-osteoarthritis-synovitis-and-wound-repair
#14
Chia-Lung Wu, Kelly A Kimmerling, Dianne Little, Farshid Guilak
High-fat diet-induced obesity is a major risk factor for osteoarthritis (OA) and diminished wound healing. The objective of this study was to determine the associations among serum and synovial fluid lipid levels with OA, synovitis, adipokine levels, and wound healing in a pre-clinical obese mouse model of OA. Male C57BL/6 J mice were fed either a low-fat (10% kcal) or one of three high-fat (HF, 60% kcal) diets rich in saturated fatty acids (SFAs), ω-6 or ω-3 polyunsaturated FAs (PUFAs). OA was induced by destabilization of the medial meniscus...
March 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28287268/nmr-based-lipid-profiling-of-high-density-lipoprotein-particles-in-healthy-subjects-with-low-normal-and-elevated-hdl-cholesterol
#15
Christina E Kostara, Vasilis Tsimihodimos, Moses S Elisaf, Eleni T Bairaktari
Recent studies suggest that the cholesterol content of HDL (high density lipoproteins) may provide limited information on their antiatherogenic properties and that the composition and particles' structure provide more information on their functionality. We used NMR-based (nuclear magnetic resonance-based) lipidomics to study the relationships of serum HDL-C (HDL-cholesterol) levels with the lipid composition of HDL particles in three groups of subjects selected on the basis of their HDL-C levels. Subjects with low and high HDL-C levels exhibited differences in HDL lipidome compared to those with normal HDL-C levels...
March 21, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28287094/enlightening-discriminative-network-functional-modules-behind-principal-component-analysis-separation-in-differential-omic-science-studies
#16
Sara Ciucci, Yan Ge, Claudio Durán, Alessandra Palladini, Víctor Jiménez-Jiménez, Luisa María Martínez-Sánchez, Yuting Wang, Susanne Sales, Andrej Shevchenko, Steven W Poser, Maik Herbig, Oliver Otto, Andreas Androutsellis-Theotokis, Jochen Guck, Mathias J Gerl, Carlo Vittorio Cannistraci
Omic science is rapidly growing and one of the most employed techniques to explore differential patterns in omic datasets is principal component analysis (PCA). However, a method to enlighten the network of omic features that mostly contribute to the sample separation obtained by PCA is missing. An alternative is to build correlation networks between univariately-selected significant omic features, but this neglects the multivariate unsupervised feature compression responsible for the PCA sample segregation...
March 13, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28265968/expanding-lipidome-coverage-using-lc-ms-ms-data-dependent-acquisition-with-automated-exclusion-list-generation
#17
Jeremy P Koelmel, Nicholas M Kroeger, Emily L Gill, Candice Z Ulmer, John A Bowden, Rainey E Patterson, Richard A Yost, Timothy J Garrett
Untargeted omics analyses aim to comprehensively characterize biomolecules within a biological system. Changes in the presence or quantity of these biomolecules can indicate important biological perturbations, such as those caused by disease. With current technological advancements, the entire genome can now be sequenced; however, in the burgeoning fields of lipidomics, only a subset of lipids can be identified. The recent emergence of high resolution tandem mass spectrometry (HR-MS/MS), in combination with ultra-high performance liquid chromatography, has resulted in an increased coverage of the lipidome...
May 2017: Journal of the American Society for Mass Spectrometry
https://www.readbyqxmd.com/read/28251853/uplc-qqq-ms-based-lipidomics-approach-to-characterize-lipid-alterations-in-inflammatory-macrophages
#18
Jae Won Lee, Hyuck Jun Mok, Dae Young Lee, Seung Cheol Park, Geum-Soog Kim, Seung-Eun Lee, Young-Seob Lee, Kwang Pyo Kim, Hyung Don Kim
In this study, UPLC-QqQ/MS-based lipidomics was applied to profile various lipids from RAW264.7 macrophages treated with different concentrations of lipopolysaccharide (LPS). The degree of inflammation increased with the LPS concentration. To elucidate the altered lipid metabolism of inflammatory macrophages, we targeted to analyze 25 lipid classes from LPS-treated RAW264.7 cells. As a result, 523 lipid species were successfully profiled by using the optimal UPLC and MRM. Statistical data analyses such as PCA, PLS-DA, and HCA differentiated five RAW264...
March 16, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/28251292/lipidomic-analysis-of-plasma-in-patients-with-lacunar-infarction-using-normal-phase-reversed-phase-two-dimensional-liquid-chromatography-quadrupole-time-of-flight-mass-spectrometry
#19
Li Yang, Pu Lv, Wanpeng Ai, Linnan Li, Sensen Shen, Honggang Nie, Yabing Shan, Yu Bai, Yining Huang, Huwei Liu
Stroke is a major cause of mortality and long-term disability worldwide. The study of biomarkers and pathogenesis is vital for early diagnosis and treatment of stroke. In the present study, a continuous-flow normal-phase/reversed-phase two-dimensional liquid chromatography-quadrupole time-of-flight mass spectrometry (NP/RP 2D LC-QToF/MS) method was employed to measure lipid species in human plasma, including healthy controls and lacunar infarction (LI) patients. As a result, 13 lipid species were demonstrated with significant difference between the two groups, and a "plasma biomarker model" including glucosylceramide (38:2), phosphatidylethanolamine (35:2), free fatty acid (16:1), and triacylglycerol (56:5) was finally established...
March 1, 2017: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/28249170/plasma-dihydroceramides-are-diabetes-susceptibility-biomarker-candidates-in-mice-and-humans
#20
Leonore Wigger, Céline Cruciani-Guglielmacci, Anthony Nicolas, Jessica Denom, Neïké Fernandez, Frédéric Fumeron, Pedro Marques-Vidal, Alain Ktorza, Werner Kramer, Anke Schulte, Hervé Le Stunff, Robin Liechti, Ioannis Xenarios, Peter Vollenweider, Gérard Waeber, Ingo Uphues, Ronan Roussel, Christophe Magnan, Mark Ibberson, Bernard Thorens
Plasma metabolite concentrations reflect the activity of tissue metabolic pathways and their quantitative determination may be informative about pathogenic conditions. We searched for plasma lipid species whose concentrations correlate with various parameters of glucose homeostasis and susceptibility to type 2 diabetes (T2D). Shotgun lipidomic analysis of the plasma of mice from different genetic backgrounds, which develop a pre-diabetic state at different rates when metabolically stressed, led to the identification of a group of sphingolipids correlated with glucose tolerance and insulin secretion...
February 28, 2017: Cell Reports
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