keyword
MENU ▼
Read by QxMD icon Read
search

UBC

keyword
https://www.readbyqxmd.com/read/29324722/evaluation-of-a-new-survivin-elisa-and-ubc%C3%A2-rapid-for-the-detection-of-bladder-cancer-in-urine
#1
Jan Gleichenhagen, Christian Arndt, Swaantje Casjens, Carmen Meinig, Holger Gerullis, Irina Raiko, Thomas Brüning, Thorsten Ecke, Georg Johnen
Urine-based biomarkers for non-invasive diagnosis of bladder cancer are urgently needed. No single marker with sufficient sensitivity and specificity has been described so far. Thus, a combination of markers appears to be a promising approach. The aim of this case-control study was to evaluate the performance of an in-house developed enzyme-linked immunosorbent assay (ELISA) for survivin, the UBC®Rapid test, and the combination of both assays. A total of 290 patients were recruited. Due to prior bladder cancer, 46 patients were excluded...
January 11, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29316780/psen1-p-met233val-in-a-complex-neurodegenerative-movement-and-neuropsychiatric-disorder
#2
Silke Appel-Cresswell, Ilaria Guella, Anna Lehman, Dean Foti, Matthew J Farrer
Mutations in presenilin 1 (PSEN1) are the most common cause of autosomal dominant Alzheimer's disease. Here, we report a Canadian-Vietnamese family carrying a PSEN1 p.Met233Val mutation with an exceptionally early and severe presentation that includes a wide range of atypical symptoms, including prominent ataxia, Parkinsonism, spasticity, dystonia, action tremor, myoclonus, bulbar symptoms, seizures, hallucinations and behavioral changes. Whole-exome sequencing (WES) was performed on the affected proband after many assessments over several years proved diagnostically inconclusive...
January 11, 2018: Journal of Movement Disorders
https://www.readbyqxmd.com/read/29291482/paraneoplastic-neuromyelitis-optica-spectrum-disorder-a-single-center-cohort-description-with-two-cases-of-histological-validation
#3
Philippe Beauchemin, Raffaele Iorio, Anthony L Traboulsee, Thalia Field, Anna V Tinker, Robert L Carruthers
BACKGROUND: Paraneoplastic syndromes are remote effects of cancer caused by an autoimmune response triggered by tumor cells. Paraneoplastic Neuromyelitis Optica Spectrum Disorders (NMOSD) has been previously described, but the underlying mechanism for these rare cases is not well characterized. This paper presents a newly described case series of paraneoplastic NMOSD, including 2 new histological types of cancer and histological validation. METHODS: The UBC NMO clinic database was surveyed and identified 6 patients with paraneoplastic NMOSD...
December 23, 2017: Multiple Sclerosis and related Disorders
https://www.readbyqxmd.com/read/29288002/m2-macrophage-infiltration-and-macrophage-traits-of-tumor-cells-in-urinary-bladder-cancer
#4
Firas Aljabery, Hans Olsson, Oliver Gimm, Staffan Jahnson, Ivan Shabo
BACKGROUND: Tumor-associated macrophages (TAMs) constitute a subset of nonneoplastic cells in tumor stroma and influence cancer progression in solid tumors. The clinical significance of TAMs in urinary bladder cancer (UBC) is controversial. METHODS: We prospectively studied 103 patients with stage pT1-T4 UBC treated with cystectomy and pelvic lymph node dissection. Tumor sections were immunostained with M2-specific macrophage marker CD163 and proliferation marker Ki-67...
December 26, 2017: Urologic Oncology
https://www.readbyqxmd.com/read/29247242/myeloid-differentiation-primary-response-gene-myd-88-signalling-is-not-essential-for-intestinal-fibrosis-development
#5
C Lutz, B Weder, A Hünerwadel, S Fagagnini, B Lang, N Beerenwinkel, J B Rossel, G Rogler, B Misselwitz, M Hausmann
Dysregulation of the immune response to microbiota is associated with inflammatory bowel disease (IBD), which can trigger intestinal fibrosis. MyD88 is a key component of microbiota signalling but its influence on intestinal fibrosis has not been clarified. Small bowel resections from donor-mice were transplanted subcutaneously into the neck of recipients C57BL/6 B6-MyD88tm1 Aki (MyD88-/-) and C57BL/6-Tg(UBC-green fluorescence protein (GFP))30Scha/J (GFP-Tg). Grafts were explanted up to 21 days after transplantation...
December 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29244872/the-ubiquitin-proteasome-system-is-required-for-african-swine-fever-replication
#6
Lucía Barrado-Gil, Inmaculada Galindo, Diego Martínez-Alonso, Sergio Viedma, Covadonga Alonso
Several viruses manipulate the ubiquitin-proteasome system (UPS) to initiate a productive infection. Determined viral proteins are able to change the host's ubiquitin machinery and some viruses even encode their own ubiquitinating or deubiquitinating enzymes. African swine fever virus (ASFV) encodes a gene homologous to the E2 ubiquitin conjugating (UBC) enzyme. The viral ubiquitin-conjugating enzyme (UBCv1) is expressed throughout ASFV infection and accumulates at late times post infection. UBCv is also present in the viral particle suggesting that the ubiquitin-proteasome pathway could play an important role at early ASFV infection...
2017: PloS One
https://www.readbyqxmd.com/read/29226853/clinical-and-rna-expression-integrated-signature-for-urothelial-bladder-cancer-prognosis
#7
Jie Xiong, Ke Xiong, Zhitong Bing
BACKGROUND: Accumulating evidence shows that clinical factors alone are not adequate for predicting the survival of patients with urothelial bladder cancer (UBC), and many genes have been found to be associated with UBC prognosis. PURPOSE: The objective of this study is to develop a signature which integrates clinical and molecular information to predict the overall survival of UBC patients more accurate. MATERIALS AND METHODS: We integrated messenger RNA (mRNA) and microRNA (miRNA) expression profiles and the corresponding clinical data of 402 UBC patients and 19 normal controls from The Cancer Genome Atlas...
November 25, 2017: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/29212158/rnascope-in-situ-hybridization-confirms-mrna-integrity-in-formalin-fixed-paraffin-embedded-cancer-tissue-samples
#8
Victoria Bingham, Leanne McIlreavey, Christine Greene, Edwina O'Doherty, Rebecca Clarke, Stephanie Craig, Manuel Salto-Tellez, Stephen McQuaid, Claire Lewis, Jacqueline James
Immunohistochemistry remains the overwhelming technique of choice for test biomarker evaluation in both clinical or research settings when using formalin-fixed, paraffin embedded tissue sections. However, validations can be complex with significant issues about specificity, sensitivity and reproducibility. The vast array of commercially available antibodies from many vendors may also lead to non-standard approaches which are difficult to cross-reference. In contrast mRNA detection, by in situ hybridization (ISH) with sequence specific probes, offers a realistic alternative, with less validation steps and more stringent and reproducible assessment criteria...
November 7, 2017: Oncotarget
https://www.readbyqxmd.com/read/29208024/selection-of-a-suitable-reference-gene-for-quantitative-gene-expression-in-mouse-lymph-nodes-after-vaccination
#9
Yung-Yi C Mosley, Harm HogenEsch
BACKGROUND: The quantification of gene expression is an important tool in the evaluation of the immune response to vaccines. Reliable reference genes for gene expression studies in mouse draining lymph nodes after vaccination have not been reported. RESULTS: The utility of seven potential reference genes was investigated using commercially available Taq-man primer/probe mixes. Results were evaluated with RefFinder, a web-based program including multiple algorithm methods such as geNorm, NormFinder, BestKeeper and the comparative delta-Ct...
December 6, 2017: BMC Research Notes
https://www.readbyqxmd.com/read/29187903/mir34a-golph3-axis-abrogates-urothelial-bladder-cancer-chemoresistance-via-reduced-cancer-stemness
#10
Qing Zhang, Junlong Zhuang, Yongming Deng, Lin Yang, Wenmin Cao, Wei Chen, Tingsheng Lin, Xiaoyu Lv, Hang Yu, Yanshi Xue, Hongqian Guo
Rationale: Chemoresistance and subsequent recurrence of human urothelial bladder cancer (UBC) is partially driven by a subpopulation of tumor initiating cells, namely cancer stem cells (CSCs). However, the underlying molecular mechanism in chemotherapy-induced CSCs enrichment and following chemoresistance and recurrence remains largely unclear. Methods: Gemcitabine and cisplatin (GC) chemoresistant cell lines (T24 GC 3rd and 5637 GC 3rd cells) and the chemo-sensitive UBC cell lines T24 and 5637 were established in vivo for the investigation of acquired resistance mechanisms...
2017: Theranostics
https://www.readbyqxmd.com/read/29187470/the-genomic-alterations-of-5%C3%AE-reductases-and-their-inhibitor-finasteride-s-effect-in-bladder-cancer
#11
Chi-Cheng Chen, Chi-Ping Huang, Yi-Tung Tsai, Teng-Fu Hseih, Chih-Rong Shyr
BACKGROUND/AIM: Since androgens affect urothelial bladder cancer (UBC), we examined whether 5α-reductases (5-AR) have genomic alterations in UBC and whether 5α-reductase inhibitors (5-ARI) affect UBC. MATERIALS AND METHODS: The cBioPortal was used to analyze genomic alternations of 5-ARs in UBC cancer genomic datasets. Next, we used the Taiwan National Health Insurance Research database to conduct a population-based case-control study to investigate the effect of a 5-ARI, finasteride on UBC incidence...
December 2017: Anticancer Research
https://www.readbyqxmd.com/read/29166265/selection-of-reference-genes-for-quantitative-real-time-rt-pcr-on-gene-expression-in-golden-pompano-trachinotus-ovatus
#12
X J Chen, X Q Zhang, S Huang, Z J Cao, Q W Qin, W T Hu, Y Sun, Y C Zhou
Golden pompano (Trachinotus ovatus) is an important economically fish species. In this study, with an aim to identify reliable reference genes for quantitative real-time PCR (qRT-PCR) in golden pompano, we evaluated the expression stability of eight housekeeping genes in the presence and absence of poly I:C stimulation in eight tissues. The PCR data was analyzed by geNorm and NormFinder algorithms. The results showed that the expression of all the examined genes exhibited tissue-dependent variations. When under normal physiological condition, geNorm and NormFinder identified B2M and 18S as suitable genes...
September 26, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/29163683/knockdown-of-hmgn5-increases-the-chemosensitivity-of-human-urothelial-bladder-cancer-cells-to-cisplatin-by-targeting-pi3k-akt-signaling
#13
Yu Gan, Leye He, Kun Yao, Jing Tan, Qing Zeng, Yingbo Dai, Jianye Liu, Yuxin Tang
High-mobility group nucleosome-binding domain 5 (HMGN5) is the latest member of the HMGN family of proteins. Numerous studies have confirmed the carcinogenic role of HMGN5 in cancer, but its function in the regulation of chemosensitivity is largely unknown and controversial. A previous study by the authors of the present study demonstrated that HMGN5 contributes to the progression of urothelial bladder cancer (UBC) through regulating the expression of E-cadherin and vascular endothelial growth factor (VEGF)-C, which are associated with the sensitivity of tumor cells to cisplatin...
December 2017: Oncology Letters
https://www.readbyqxmd.com/read/29152550/tertiary-lymphoid-structures-associate-with-tumour-stage-in-urothelial-bladder-cancer
#14
Madhuri Koti, Amanda Shou Xu, Kevin Yi Mi Ren, Kash Visram, Runhan Ren, David M Berman, D Robert Siemens
Background: Urothelial bladder cancer (UBC) is a highly prevalent disease in North America, however its optimal management remains elusive. The contribution of B cell associated responses is poorly understood in bladder cancer. Lymphoid neogenesis is a hallmark of an active immune response at tumor sites that sometimes leads to formation of tertiary lymphoid structures (TLS) that resemble germinal centers formed in secondary lymphoid organs. Objective: This study was conducted with an aim to investigate the presence and characteristics of TLS in UBC with a focus to compare and contrast the TLS formation in treatment naive low grade non-muscle invasive (NMIBC) and muscle invasive bladder cancers (MIBC)...
October 27, 2017: Bladder Cancer
https://www.readbyqxmd.com/read/29139108/a-3-untranslated-region-polymorphism-rs2304277-in-the-dna-repair-pathway-gene-ogg1-is-a-novel-risk-modulator-for-urothelial-bladder-carcinoma
#15
Tayyaba Ahmed, Saira Nawaz, Rabia Noreen, Kashif Sardar Bangash, Abdur Rauf, Muhammad Younis, Khursheed Anwar, Muhammad Athar Khawaja, Maleeha Azam, Abid Ali Qureshi, Saeed Akhter, Lambertus A Kiemeney, Raheel Qamar, Syeda Hafiza Benish Ali
Altered DNA repair capacity may affect an individual's susceptibility to cancers due to compromised genomic integrity. This study was designed to elucidate the association of selected polymorphisms in DNA repair genes with urothelial bladder carcinoma (UBC). OGG1 rs1052133 and rs2304277, XRCC1 rs1799782 and rs25487, XRCC3 rs861539, XPC rs2228001, and XPD rs13181 were genotyped using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in 200 UBC cases and 200 controls. We found association of OGG1 rs2304277 [odds ratio (OR)GG = 3...
November 15, 2017: Annals of Human Genetics
https://www.readbyqxmd.com/read/29130938/ubiquitin-levels-the-next-target-against-gynecological-cancers
#16
Diane L Haakonsen, Michael Rape
Ubiquitylation is a tightly regulated process that is essential for appropriate cell survival and function, and the ubiquitin pathway has shown promise as a therapeutic target for several forms of cancer. In this issue of the JCI, Kedves and colleagues report the identification of a subset of gynecological cancers with repressed expression of the polyubiquitin gene UBB, which renders these cancer cells sensitive to further decreases in ubiquitin production by inhibition of the polyubiquitin gene UBC. Moreover, inducible depletion of UBC in mice harboring tumors with low UBB levels dramatically decreased tumor burden and prolonged survival...
November 13, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29130934/recurrent-ubiquitin-b-silencing-in-gynecological-cancers-establishes-dependence-on-ubiquitin-c
#17
Alexia T Kedves, Scott Gleim, Xiaoyou Liang, Dennis M Bonal, Frederic Sigoillot, Fred Harbinski, Sneha Sanghavi, Christina Benander, Elizabeth George, Prafulla C Gokhale, Quang-De Nguyen, Paul T Kirschmeier, Robert J Distel, Jeremy Jenkins, Michael S Goldberg, William C Forrester
Transcriptional repression of ubiquitin B (UBB) is a cancer-subtype-specific alteration that occurs in a substantial population of patients with cancers of the female reproductive tract. UBB is 1 of 2 genes encoding for ubiquitin as a polyprotein consisting of multiple copies of ubiquitin monomers. Silencing of UBB reduces cellular UBB levels and results in an exquisite dependence on ubiquitin C (UBC), the second polyubiquitin gene. UBB is repressed in approximately 30% of high-grade serous ovarian cancer (HGSOC) patients and is a recurrent lesion in uterine carcinosarcoma and endometrial carcinoma...
November 13, 2017: Journal of Clinical Investigation
https://www.readbyqxmd.com/read/29130497/ubc-nepal-expedition-acclimatization-to-high-altitude-increases-spinal-motoneurone-excitability-during-fatigue-in-humans
#18
Luca Ruggiero, Alexandra F Yacyshyn, Jane Nettleton, Chris J McNeil
The fatigue-induced failure of the motor cortex to drive muscles maximally increases in acute hypoxia (AH) compared to normoxia (N) but improves with acclimatization (chronic hypoxia; CH). Despite their importance to muscle output, it is unknown how locomotor motoneurones in humans are affected by hypoxia and acclimatization. Eleven participants performed 16 min of submaximal (25% maximal torque, MVC) intermittent isometric elbow flexions in N, AH (environmental chamber), and CH (7-14 days at 5050 m) (PI O2 =  140, 74, and 76 mmHg, respectively)...
November 11, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/29092895/ubiquitination-of-the-pi3-kinase-vps-34-promotes-vps-34-stability-and-phagosome-maturation
#19
Jinchao Liu, Meijiao Li, Lin Li, She Chen, Xiaochen Wang
Apoptotic cells generated by programmed cell death are engulfed by phagocytes and enclosed within membrane-bound phagosomes. Maturation of apoptotic cell-containing phagosomes leads to formation of phagolysosomes where cell corpses are degraded. The class III phosphatidylinositol 3-kinase (PI3-kinase) VPS-34 coordinates with PIKI-1, a class II PI3-kinase, to produce PtdIns3P on phagosomes, thus promoting phagosome closure and maturation. Here, we identified UBC-13, an E2 ubiquitin-conjugating enzyme that functions in the same pathway with VPS-34 but in parallel to PIKI-1 to regulate PtdIns3P generation on phagosomes...
November 1, 2017: Journal of Cell Biology
https://www.readbyqxmd.com/read/29080905/increased-cocaine-and-amphetamine-regulated-transcript-cord-blood-levels-in-the-newborns-exposed-to-crack-cocaine-in-utero
#20
Rodrigo Ritter Parcianello, Victor Mardini, Keila Maria Mendes Ceresér, Daniel D Langleben, Fernando Xavier, Maria Lucrécia Scherer Zavaschi, Luis Augusto Paim Rhode, Flávio Pechansky, Carolina Gubert, Claudia Maciel Szobot
BACKGROUND: Cocaine and amphetamine-regulated transcript (CART) is an endogenous antioxidant present since the embryonic period. CART is activated by high levels of dopamine and might be of interested in understanding the changes in the REDOX system associated with crack/cocaine intake. The goal of this study was to determine whether exposure to crack in utero is associated with increased CART levels. METHODS: In this cross-sectional study with consecutive sampling, we compared the umbilical cord blood (UCB) CART levels (μg/mL) of newborns exposed to crack/cocaine in utero (EN, n = 57) to levels in non-exposed newborns (NEN, n = 99)...
October 28, 2017: Psychopharmacology
keyword
keyword
78712
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"