keyword
https://read.qxmd.com/read/38535345/inhibition-of-rice-stripe-virus-accumulation-by-polyubiquitin-c-in-laodelphax-striatellus
#1
JOURNAL ARTICLE
Bo-Xue Li, Yu-Hua Qi, Chuan-Xi Zhang, Jian-Ping Chen, Jun-Min Li, Gang Lu
Many hosts utilize the ubiquitin system to defend against viral infection. As a key subunit of the ubiquitin system, the role of polyubiquitin in the viral infection of insects is unclear. Here, we identified the full-length cDNA of the polyubiquitin-C (UBC) gene in Laodelphax striatellus , the small brown planthopper (SBPH). LsUBC was expressed in various tissues and was highly expressed in salivary glands, midgut, and reproductive systems. Furthermore, the LsUBC expression profiles in the developmental stages showed that LsUBC was ubiquitously expressed in seven developmental stages and was highest expressed in female adults with SBPH...
February 22, 2024: Insects
https://read.qxmd.com/read/38531255/on-relevant-features-for-the-recurrence-prediction-of-urothelial-carcinoma-of-the-bladder
#2
JOURNAL ARTICLE
Louisa Schwarz, Dominik Sobania, Franz Rothlauf
BACKGROUND: Urothelial bladder cancer (UBC) is characterized by a high recurrence rate, which is predicted by scoring systems. However, recent studies show the superiority of Machine Learning (ML) models. Nevertheless, these ML approaches are rarely used in medical practice because most of them are black-box models, that cannot adequately explain how a prediction is made. OBJECTIVE: We investigate the global feature importance of different ML models. By providing information on the most relevant features, we can facilitate the use of ML in everyday medical practice...
March 18, 2024: International Journal of Medical Informatics
https://read.qxmd.com/read/38527945/a-comparative-assessment-of-reference-genes-in-mouse-brown-adipocyte-differentiation-and-thermogenesis-in-vitro
#3
JOURNAL ARTICLE
Trang Huyen Lai, Jin Seok Hwang, Quang Nhat Ngo, Dong-Kun Lee, Hyun Joon Kim, Deok Ryong Kim
Adipogenic differentiation and thermogenesis in brown adipose tissue (BAT) undergo dynamic processes, altering phenotypes and gene expressions. Proper reference genes in gene expression analysis are crucial to mitigate experimental variances and ensure PCR efficacy. Unreliable reference genes can lead to erroneous gene expression quantification, resulting in data misinterpretation. This study focused on identifying suitable reference genes for mouse brown adipocyte research, utilizing brown adipocytes from the Ucp1-luciferase ThermoMouse model...
December 2024: Adipocyte
https://read.qxmd.com/read/38521702/construction-and-validation-of-a-nomogram-predicting-the-overall-survival-benefit-of-unilateral-breast-cancer-patients-undergoing-contralateral-prophylactic-mastectomy
#4
JOURNAL ARTICLE
Luyao Dai, Wenyu Yan, Xueting Ren, Dandan Liu, Lidan Chang, Shuai Lin, Hao Wu, Huafeng Kang, Xiaobin Ma
BACKGROUND: Currently, research on the prognostic factors of unilateral breast cancer (UBC) patients receiving contralateral prophylactic mastectomy (CPM) is limited. This study aimed to construct a new nomogram to predict these patients' overall survival (OS). METHODS: In this retrospective study, 88,477 patients who underwent CPM or unilateral mastectomy (UM) were selected from the Surveillance, Epidemiology, and End Results database. Kaplan-Meier curves and Cox regression analyses were used to determine the difference in the impact of the 2 surgical methods on the prognosis...
February 7, 2024: Clinical Breast Cancer
https://read.qxmd.com/read/38514751/cross-sectional-and-longitudinal-analyses-of-urinary-extracellular-vesicle-mrna-markers-in-urothelial-bladder-cancer-patients
#5
JOURNAL ARTICLE
Taku Murakami, Keita Minami, Toru Harabayashi, Satoru Maruyama, Norikata Takada, Akira Kashiwagi, Haruka Miyata, Yasuyuki Sato, Ryuji Matsumoto, Hiroshi Kikuchi, Takashige Abe, Yoichi M Ito, Sachiyo Murai, Nobuo Shinohara, Hiroshi Harada, Takahiro Osawa
We designed this multi-center prospective study with the following objectives: (1) the cross-sectional validation of extracellular vesicles (EV) mRNA markers to detect urothelial bladder cancer (UBC) before transurethral resection of bladder cancer (TURBT), and (2) the longitudinal validation of EV mRNA markers to monitor non-muscle invasive bladder cancer (NMIBC) recurrence after TURBT. EV mRNA markers evaluated in this study were KRT17, GPRC5A, and SLC2A1 in addition to two additional markers from literatures, MDK and CXCR2, and measured by quantitative RT-PCR with normalization by a reference gene (ALDOB)...
March 21, 2024: Scientific Reports
https://read.qxmd.com/read/38486003/screening-and-validation-of-reference-genes-in-dracaena-cochinchinensis-using-quantitative-real-time-pcr
#6
JOURNAL ARTICLE
Shixi Gao, Junxiang Peng, Mei Rong, Yang Liu, Yanhong Xu, Jianhe Wei
Dragon's blood, the red resin derived from the wounded Dracaena, is a precious traditional medicine used by different culture. Dracaena cochinchinensis is one of the main species of Dracaena, and is the endangered medicinal plants in China. The vulnerable status severely limits the medicinal value and wide application of dragon's blood. Therefore, it's essential to analyze the mechanisms that form dragon's blood in order to increase artificial production. To clarify the mechanisms forming dragon's blood, understanding gene expression in the flavonoid biosynthesis pathway is the foundation...
March 14, 2024: Scientific Reports
https://read.qxmd.com/read/38480976/adeno-associated-virus-mediated-trastuzumab-delivery-to-the-central-nervous-system-for-human-epidermal-growth-factor-receptor-2-brain-metastasis
#7
JOURNAL ARTICLE
Marcela S Werner, Shweta Aras, Ashleigh R Morgan, Jillian Roamer, Nesteene J Param, Kanyin Olagbegi, R Jason Lamontagne, Jenny A Greig, James M Wilson
Trastuzumab improves overall survival for HER2+ breast cancer, but its short half-life in the cerebrospinal fluid (~2-4 days) and delivery limitations restrict the ability to target HER2+ central nervous system (CNS) disease. We developed an adeno-associated virus (AAV) vector expressing a codon-optimized, ubiquitin C (UbC)-promoter-driven trastuzumab sequence (AAV9.UbC.trastuzumab) for intrathecal administration. Transgene expression was evaluated in adult Rag1 knockout mice and rhesus nonhuman primates (NHPs) after a single intracerebroventricular (ICV) or intra-cisterna magna (ICM) AAV9...
March 13, 2024: Cancer Gene Therapy
https://read.qxmd.com/read/38476605/exploring-protein-profiles-and-hub-genes-in-ameloblastoma
#8
JOURNAL ARTICLE
Sirima Sanguansin, Sudaporn Kengkarn, Boworn Klongnoi, Suthipong Chujan, Sittirak Roytrakul, Nakarin Kitkumthorn
Ameloblastoma (AM) is a prominent benign odontogenic tumor characterized by aggressiveness, likely originating from tooth-generating tissue or the dental follicle (DF). However, proteomic distinctions between AM and DF remain unclear. In the present study, the aim was to identify the distinction between AM and DF in terms of their proteome and to determine the associated hub genes. Shotgun proteomics was used to compare the proteomes of seven fresh-frozen AM tissues and five DF tissues. Differentially expressed proteins (DEPs) were quantified and subsequently analyzed through Gene Ontology-based functional analysis, protein-protein interaction (PPI) analysis and hub gene identification...
April 2024: Biomedical Reports
https://read.qxmd.com/read/38473264/ubiquitin-b-ubiquitin-c-and-%C3%AE-catenin-as-promising-diagnostic-and-prognostic-tools-in-prostate-cancer
#9
JOURNAL ARTICLE
Daria Piątkowska, Anna Klimaszewska-Wiśniewska, Alicja Kosińska, Radosław Wujec, Dariusz Grzanka, Justyna Durślewicz
Prostate cancer (PC) is a major global public health concern, imposing a significant burden on men and ranking as the second most prevalent malignancy. This study delves into the intricate world of ubiquitination processes and expression regulation, with a specific focus on understanding the roles of ubiquitin B (UBB), ubiquitin C (UBC), and β-Catenin in PC development. We thoroughly analyze the expression profiles of UBB, UBC, and β-Catenin, investigating their interactions and associations with clinical and histopathological data...
February 23, 2024: Cancers
https://read.qxmd.com/read/38456745/mri-based-machine-learning-radiomics-for-preoperative-assessment-of-human-epidermal-growth-factor-receptor-2-status-in-urothelial-bladder-carcinoma
#10
JOURNAL ARTICLE
Ruixi Yu, Lingkai Cai, Yuxi Gong, Xueying Sun, Kai Li, Qiang Cao, Xiao Yang, Qiang Lu
BACKGROUND: The human epidermal growth factor receptor 2 (HER2) has recently emerged as hotspot in targeted therapy for urothelial bladder cancer (UBC). The HER2 status is mainly identified by immunohistochemistry (IHC), preoperative and noninvasive methods for determining HER2 status in UBC remain in searching. PURPOSES: To investigate whether radiomics features extracted from MRI using machine learning algorithms can noninvasively evaluate the HER2 status in UBC...
March 8, 2024: Journal of Magnetic Resonance Imaging: JMRI
https://read.qxmd.com/read/38449944/diagnosis-and-current-treatment-of-aneurysmal-bone-cysts
#11
REVIEW
Khalid A Bakarman
The purpose of this review is to increase awareness about the evolution and development of current trends in the diagnosis and treatment of aneurysmal bone cysts (ABCs). ABCs are benign, but locally aggressive bone tumors that mainly affect children. ABCs comprise 1% of all primary bone tumors and occur most frequently during the first two decades of life. The diagnosis is made using a variety of imaging modalities and has the characteristic features of an expansile, radiolucent lesion that is often seen in the metaphyseal region of the bone and has fluid-fluid levels that are apparent on MRI...
February 2024: Curēus
https://read.qxmd.com/read/38443694/transcriptome-profiling-and-metabolic-pathway-analysis-towards-reliable-biomarker-discovery-in-early-stage-lung-cancer
#12
JOURNAL ARTICLE
Muthu Kumar Thirunavukkarasu, Priyanka Ramesh, Ramanathan Karuppasamy, Shanthi Veerappapillai
Earlier diagnosis of lung cancer is crucial for reducing mortality and morbidity in high-risk patients. Liquid biopsy is a critical technique for detecting the cancer earlier and tracking the treatment outcomes. However, noninvasive biomarkers are desperately needed due to the lack of therapeutic sensitivity and early-stage diagnosis. Therefore, we have utilized transcriptomic profiling of early-stage lung cancer patients to discover promising biomarkers and their associated metabolic functions. Initially, PCA highlights the diversity level of gene expression in three stages of lung cancer samples...
March 5, 2024: Journal of Applied Genetics
https://read.qxmd.com/read/38435489/contralateral-prophylactic-mastectomy-in-a-rural-population-a-single-institution-experience
#13
JOURNAL ARTICLE
Devin J Clegg, Erica N Whiteaker, Brett J Salomon, Kaylan N Gee, Christopher G Porter, Thomas W Mazonas, R Eric Heidel, Ashton J Brooks, John L Bell, Stefanos Boukovalas, Jillian M Lloyd
BACKGROUND: The incidence of contralateral prophylactic mastectomy (CPM) for unilateral breast cancer (UBC) has continued to increase, despite an absent survival benefit except in populations at highest risk for developing contralateral breast cancer (CBC). CPM rates may be higher in rural populations but causes remain unclear. A study performed at our institution previously found that 21.8 % of patients with UBC underwent CPM from 2000 to 2009. This study aimed to evaluate the CPM trend at a single institution serving a rural population and identify the CPM rate in average-risk patients...
March 2024: Surgery open science
https://read.qxmd.com/read/38409561/treatment-of-unicameral-bone-cysts-utilizing-the-sclerograft%C3%A2-technique
#14
JOURNAL ARTICLE
Shankar Rajeswaran, Michelle Wiese, Joe Baker, Julie Chesterton, Jonathan Samet, Jared Green, Ahsun Riaz, Samdeep Mouli, Bartley Thornburg, Samer Attar, Terrance Peabody, James Donaldson
PURPOSE: To evaluate the Sclerograft™ procedure, which is an image-guided, minimally invasive approach of chemical sclerotherapy followed by bone grafting of unicameral bone cysts (UBC). MATERIALS AND METHODS: A retrospective evaluation from August 2018 through August 2023 was performed at a single institution on patients that underwent the Sclerograft™ procedure for UBCs. Radiographic healing was evaluated utilizing the Modified Neer Classification...
February 26, 2024: Cardiovascular and Interventional Radiology
https://read.qxmd.com/read/38403529/simulation-of-the-effects-of-molecular-urine-markers-in-follow-up-of-patients-with-high-risk-non-muscle-invasive-bladder-cancer
#15
REVIEW
Natalya Benderska-Söder, Thorsten Ecke, Lisa Kleinlein, Florian Roghmann, Ekkehardt Bismarck, Bas W G van Rhijn, Arnulf Stenzl, Johannes Alfred Witjes, Tilman Todenhöfer, Oliver W Hakenberg, Marc Oliver Grimm, Peter J Goebell, Maximilian Burger, Jorgen Bjerggaard Jensen, Bernd J Schmitz-Dräger
A plethora of urine markers for the management of patients with bladder cancer has been developed and studied in the past. However, the clinical impact of urine testing on patient management remains obscure. The goal of this manuscript is to identify scenarios for the potential use of molecular urine markers in the follow-up of patients with high-risk non-muscle-invasive BC (NMIBC) and estimate potential risks and benefits. Information on the course of disease of patients with high-risk NMIBC and performance data of a point-of-care test (UBC rapid™), an MCM-5 directed ELISA (ADXBLADDER™), and 2 additional novel assays targeting alterations of mRNA expression and DNA methylation (Xpert bladder cancer monitor™, Epicheck™) were retrieved from high-quality trials and/or meta-analyses...
February 24, 2024: Urologic Oncology
https://read.qxmd.com/read/38386805/sacituzumab-govitecan-plus-platinum-based-chemotherapy-mediates-significant-antitumor-effects-in-triple-negative-breast-urinary-bladder-and-small-cell-lung-carcinomas
#16
JOURNAL ARTICLE
Thomas M Cardillo, Maria B Zalath, Roberto Arrojo, Robert M Sharkey, Serengulam V Govindan, Chien-Hsing Chang, David M Goldenberg
Sacituzumab govitecan (SG) is an antibody-drug conjugate composed of an anti-Trop-2-directed antibody conjugated with the topoisomerase I inhibitory drug, SN-38, via a proprietary hydrolysable linker. SG has received United States Food and Drug Administration (FDA) approval to treat metastatic triple-negative breast cancer (TNBC), unresectable locally advanced or metastatic hormone receptor (HR)-positive, human epidermal growth factor receptor 2 (HER2)-negative breast cancer, and accelerated approval for metastatic urothelial cancer...
February 22, 2024: Oncotarget
https://read.qxmd.com/read/38371208/diagnostic-value-of-repeated-comprehensive-investigation-with-ct-urography-and-cystoscopy-for-recurrent-macroscopic-haematuria
#17
JOURNAL ARTICLE
Suleiman Abuhasanein, Vanessa Chaves, Ali Moustafa Mohsen, Jasmine Al-Haddad, Merete Sunila, Viveka Ströck, Tomas Jerlström, Fredrik Liedberg, Jesper Swärd, Truls Gårdmark, Staffan Jahnson, Henrik Kjölhede
OBJECTIVES: To perform a descriptive analysis of a series of patients with recurrent macroscopic haematuria after a primary standard evaluation including computed tomography urography (CTU) and cystoscopy negative for urinary bladder cancer (UBC) and upper tract urothelial cancer (UTUC) and to identify potential factors associated with occurrence of recurrent macroscopic haematuria. METHODS: All patients older than 50 years who underwent urological investigation for macroscopic haematuria with both cystoscopy and CTU 2015-2017 were retrospectively reviewed...
March 2024: BJUI compass
https://read.qxmd.com/read/38371204/shortened-time-to-diagnosis-for-patients-suspected-of-urinary-bladder-cancer-managed-in-a-standardized-care-pathway-was-associated-with-an-improvement-in-tumour-characteristics
#18
JOURNAL ARTICLE
Suleiman Abuhasanein, Staffan Jahnson, Henrik Kjölhede
OBJECTIVES: To evaluate whether the implementation of standardized care pathway (SCP) for patients with suspected urinary bladder cancer (UBC) was associated with changes in tumour characteristics. Additionally, the study aims to explore whether there was a shift in the selection of patients prioritized for immediate evaluation regarding suspicion of UBC. MATERIALS AND METHODS: The study included all patients diagnosed with UBC in the NU Hospital Group between 2010 and 2019...
March 2024: BJUI compass
https://read.qxmd.com/read/38341265/selection-and-validation-of-reference-genes-for-normalization-of-gene-expression-in-floccularia-luteovirens
#19
JOURNAL ARTICLE
Yanqing Ni, Qin Zhang, Wensheng Li, Luping Cao, Rencai Feng, Zhiqiang Zhao, Xu Zhao
Floccularia luteovirens is one of the rare edible fungi with high nutritional value found on the Qinghai-Tibet Plateau. However, research at the molecular level on this species is currently constrained due to the lack of reliable reference genes for this species. Thirteen potential reference genes (ACT, GAPDH, EF-Tu, SAMDC, UBI, CLN1, β-TUB, γ-TUB, GTP, H3, UBC, UBC-E2, and GTPBP1) were chosen for the present study, and their expression under various abiotic conditions was investigated. Stability of gene expression was tested using GeNorm, NormFinder, BestKeeper, Delta-Ct, and RefFinder...
February 2024: Fungal Biology
https://read.qxmd.com/read/38341262/reference-gene-selection-for-quantitative-real-time-pcr-analysis-of-hymenopellis-radicata-under-abiotic-stress
#20
JOURNAL ARTICLE
Luping Cao, Qin Zhang, Renyun Miao, Xu Zhao, Yanqing Ni, Wensheng Li, Rencai Feng, Delong Yang
Hymenopellis radicata (H. radicata) is an edible fungus rich in protein and mineral elements, with high edible and medical value. And reference genes suitable for normalization of qRT-PCR data from this species have not been investigated. In this study, therefore, we selected 11 housekeeping genes common in biology. The expression levels of these housekeeping genes were measured in three different tissues and six different abiotic stress treatments in mycelium. They were evaluated for expression stability using online tools...
February 2024: Fungal Biology
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