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https://www.readbyqxmd.com/read/29422897/fowl-adenovirus-serotype-4-sd0828-infections-causes-high-mortality-rate-and-cytokine-levels-in-specific-pathogen-free-chickens-compared-to-ducks
#1
Rong Li, Gen Li, Jing Lin, Shaojie Han, Xiaolan Hou, Hongyu Weng, Mengjiao Guo, Zhong Lu, Ning Li, Yingli Shang, Tongjie Chai, Liangmeng Wei
Hydropericardium syndrome and inclusion body hepatitis, together called hydropericardium-hepatitis syndrome, are acute infectious diseases found in chickens. These diseases are caused primarily by fowl adenovirus serotype 4 (FAdV-4) strains. In this study, we isolated a FAdV-4 strain (SD0828) from clinically diseased chickens and phylogenetically analyzed the L1 loops of the hexon protein sequences in 3-week-old specific pathogen-free chickens and ducks infected intramuscularly and orally, determining differences in the pathogenicity by observing clinical signs and gross and histological lesions...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29413814/interferon-induced-mx-protein-from-indian-snow-trout-schizothorax-richardsonii-gray-lacks-critical-functional-features-unlike-its-mammalian-homologues
#2
Ankur Saxena, Kiran Belwal, Ankita Chauhan, Amit Pande
Viral attack within host cells triggers the production of type I interferons and leads to the induction of interferon stimulated genes (ISGs). One of the ISG Mx, encodes type I interferon inducible GTPase that is responsible for the establishment of an anti-viral state within cells. Intriguingly, several isoforms of Mx have been reported in fish, but the structural analysis of fish Mx proteins remains unexplored. For the first time, we have identified and unraveled the molecular structure of Mx protein from Indian snow trout, Schizothorax richardsonii (Gray) a Coldwater fish that inhabits the water bodies in the sub-Himalayan region...
January 31, 2018: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/29407574/pathogenicity-of-duck-hepatitis-a-virus-type-3-and-innate-immune-responses-of-the-ducklings-to-virulent-dhav-3
#3
Xuelian Zhang, Chong Cao, Zhehui Qu, Wenjing Zhang, Yue Liu, Haihui Qi, Chunxue Hao, Wenlong Zhang, Mingchun Gao, Junwei Wang, Bo Ma
Duck virus hepatitis caused by duck hepatitis A virus (DHAV) is an acute and contagious disease. To better understand the pathogenic mechanism of DHAV-3 in ducklings, an infection experiment was performed. Our results showed that typical symptoms were observed in the infected ducklings. DHAV-3 could infect many tissues, leading to pathological lesions, especially on the livers and spleen, and the host immune responses are activated in infection. Real-time quantitative PCR demonstrated that expression of many innate immune-related genes was mostly up-regulated in the livers and spleen, and antiviral innate immune response was established, but not sufficient to restrict the virus replication of lethal dose...
January 26, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29399125/identification-of-potentially-relevant-genes-for-myocardial-infarction-using-rna-sequencing-data-analysis
#4
Qiang Zhao, Ke Wu, Nannan Li, Zhengmei Li, Fenglin Jin
Myocardial infarction (MI) is a heart disease with high morbidity and mortality rates, thus it is critical to identify genes that serve roles during its pathogenesis. The objective of the present study was to identify potentially relevant genes during the progression of the disease. Blood samples from patients with MI and normal controls (n=3/group) were obtained, the RNA was extracted and cDNA libraries were established. RNA sequencing (RNA-seq) was performed on a HiSeq 2500 platform and fragments per kilobase of exon per million fragments mapped was utilized to calculate the gene expression value following preprocessing of the RNA-seq data...
February 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29343573/porcine-mx1-protein-inhibits-classical-swine-fever-virus-replication-by-targeting-nonstructural-protein-ns5b
#5
Jing Zhou, Jing Chen, Xiao-Min Zhang, Zhi-Can Gao, Chun-Chun Liu, Yun-Na Zhang, Jin-Xiu Hou, Zhao-Yao Li, Lin Kan, Wen-Liang Li, Bin Zhou
Mx proteins are interferon-induced GTPases that have broad antiviral activity against a wide range of RNA and DNA viruses; they belong to the dynamin superfamily of large GTPases. In this study, we confirmed the anti-CSFV activity of porcine Mx1 in vitro and showed that porcine Mx2 (poMx2), human MxA (huMxA), and mouse Mx1 (mmMx1) also have anti-CSFV activity in vitro. siRNA experiments revealed that depletion of endogenous poMx1 or poMx2 enhanced CSFV replication, suggesting that porcine Mx proteins are responsible for the antiviral activity of interferon-α (IFNα) against CSFV infection...
January 17, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29325821/dna-vaccine-encoding-myristoylated-membrane-protein-mmp-of-rock-bream-iridovirus-rbiv-induces-protective-immunity-in-rock-bream-oplegnathus-fasciatus
#6
Myung-Hwa Jung, Chamilani Nikapitiya, Sung-Ju Jung
Rock bream iridovirus (RBIV) causes severe mass mortalities in rock bream (Oplegnathus fasciatus) in Korea. In this study, we investigated the potential of viral membrane protein to induce antiviral status protecting rock bream against RBIV infection. We found that fish administered with ORF008L (myristoylated membrane protein, MMP) vaccine exhibited significantly higher levels of survival compared to ORF007L (major capsid protein, MCP). Moreover, ORF008L-based DNA vaccinated fish showed significant protection at 4 and 8 weeks post vaccination (wpv) than non-vaccinated fish after infected with RBIV (6...
January 8, 2018: Vaccine
https://www.readbyqxmd.com/read/29286612/unexpected-lower-expression-of-oncoprotein-gankyrin-in-drug-resistant-abcg2-overexpressing-breast-cancer-cell-lines
#7
Taheri Taheri, Khadijeh Jamialahmadi, Fatemeh Khadijeh
Background: Development of a multidrug resistance (MDR) phenotype to chemotherapy remains a major barrier in the treatment of cancer. Gankyrin (p28, p28GANK or PSMD10) is an oncoprotein overexpressed in different carcinoma cell lines. The aim of this study was to compare Gankyrin expression level in MDR cells (MCF-7/ADR and MCF-7/ MX) and non-MDR counterparts (MCF-7). Methods: Gankyrin, MDR1 (also known as ABCB1; the ATP-binding cassette sub-family B member 1) and ABCG2 (also known as BCRP; the human breast cancer resistance protein) mRNA levels were analyzed by real-time RT-PCR...
December 29, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/29278743/expression-and-purification-of-biologically-active-bovine-interferon-%C3%AE-3-il28b-in-pichia-pastoris
#8
S Barathiraja, P A V Gangadhara, V Umapathi, H J Dechamma, G R Reddy
Interferon lambda-3 (IFNλ3) which is also known as IL28B is a member of type III Interferons which are structurally and genetically different from type I Interferons. These Interferons induce signal transduction pathways similar to type I Interferons which results in the activation of Interferon Stimulated Genes (ISGs). This group of Interferons are tissue specific and reported to have antiviral activity. In the present communication, we report the expression of bovine IFNλ3 gene (coding for the mature protein) in Pichia pastoris, purification of the expressed protein and evaluation of its biological activity...
December 23, 2017: Protein Expression and Purification
https://www.readbyqxmd.com/read/29274924/vp2-pta-motif-encoding-dna-vaccine-confers-protection-against-lethal-challenge-with-infectious-pancreatic-necrosis-virus-ipnv-in-trout
#9
Sohrab Ahmadivand, Mehdi Soltani, Mahdi Behdani, Øystein Evensen, Ehsan Alirahimi, Elahe Soltani, Reza Hassanzadeh, Javad Ashrafi-Helan
IPNV in Atlantic salmon is represented by various strains with different virulence and immunogenicity linked to various motifs of the VP2 capsid. IPNV variant with P217, T221, A247 (PTA) motif is found to be avirulent in Atlantic salmon, but virulent in rainbow trout, and other salmonid species. This study describes a DNA vaccine delivered intramuscularly encoding the VP2 protein of infectious pancreatic necrosis virus (IPNV) with PTA motif that confers high protection in rainbow trout (Oncorhynchus mykiss)...
December 21, 2017: Molecular Immunology
https://www.readbyqxmd.com/read/29207185/novel-multi%C3%A2-kinase-inhibitor-t03-inhibits-taxol%C3%A2-resistant-breast-cancer
#10
Yan Li, Chunxia Liu, Ke Tang, Yan Chen, Kang Tian, Zhiqiang Feng, Jindong Chen
Activation of kinase-associated signaling pathways is one of the leading causes of various malignant phenotypes in breast tumors. Strategies of drug discovery and development have investigated approaches to target the inhibition of protein kinase signaling. In the current study, the anti‑tumor activities of a novel multi‑kinase inhibitor, T03 were evaluated in breast cancer. T03 inhibited Taxol‑resistant breast cancer cell proliferation and induced cell cycle arrest and apoptosis in vitro and in vivo...
November 28, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29017946/cloning-and-expression-of-malabar-grouper-epinephelus-malabaricus-adar1-gene-in-response-to-immune-stimulants-and-nervous-necrosis-virus
#11
Thirunavukkarasu Periyasamy, Joan Tang Xiao Joe, Ming-Wei Lu
ADARs are RNA editing catalysts that bind double-stranded RNA and convert adenosine to inosine, a process that can lead to destabilization of dsRNA structures and suppression of mRNA translation. In mammals, ADAR1 genes are involved in various cellular pathways, including interferon (IFN)-mediated response. However, the function of fish ADAR1 remains unclear. We report here the cloning of ADAR1 in Malabar grouper (Epinephelus malabaricus) (MgADAR1) and its response to various immune stimulants. The MgADAR1 cDNA is 5371-bp long, consisting of an open reading frame encoding a putative protein of 1381 amino acids, a 235-nt 5'-terminal untranslated region (UTR), and a 990-nt 3'-UTR...
October 7, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29017941/novel-ifn%C3%AE-homologue-identified-in-nile-tilapia-oreochromis-niloticus-links-with-immune-response-in-gills-under-different-stimuli
#12
Janet Velázquez, Jannel Acosta, Naylin Herrera, Antonio Morales, Osmany González, Fidel Herrera, Mario Pablo Estrada, Yamila Carpio
Interferon gamma (IFN-γ) has important roles in both innate and adaptive immune responses. This cytokine plays a very important role in defining Th1 immune response in all vertebrates. In the present study, we identified and isolated for the first time the gene coding for Nile tilapia (Oreochromis niloticus) IFNγ from spleen lymphocytes. The isolated tilapia IFNγ has between 24 and 62% of amino acid identity as compared to reported sequences for other teleost fishes. It has close phylogenetic relationships with IFNγ molecules belonging to the group of Perciforms and presents the typical structural characteristics of gamma interferon molecules...
October 7, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28990530/isoform-separation-by-a-mixed-mode-resin-toyopearl-mx-trp-650m
#13
Tsutomu Arakawa
TOYOPEARL particles are cross-linked hydroxylated methacrylic polymers available in different pore and particle sizes. They are conjugated with different ligands to generate ion-exchange, hydrophobic interaction and affinity resins. They have excellent physical and chemical properties. A mixed-mode resin, TOYOPEARL MX-Trp-650M, is made of this particle with tryptophan conjugated via N-terminal amino group and hence has both hydrophobic/aromatic side chain and carboxyl group. In this review, I will summarize the properties of the TOYOPEARL particles and MX-Trp-650M resin and application of this resin for purification of proteins and in some detail the separation of disulfide (SS)-scrambled oligomers of insulin-like growth factor-1 (IGF-1)...
October 9, 2017: Current Protein & Peptide Science
https://www.readbyqxmd.com/read/28895871/type-i-interferons-in-the-pathogenesis-and-treatment-of-canine-diseases
#14
REVIEW
Daniela Klotz, Wolfgang Baumgärtner, Ingo Gerhauser
Type I interferons (IFNs) such as IFN-α, IFN-β, IFN-ε, IFN-κ, and IFN-ω represent cytokines, which are deeply involved in the regulation and activation of innate and adaptive immune responses. They possess strong antiviral, antiproliferative, and immunomodulatory activities allowing their use in the therapy of different viral diseases, neoplasms, and immune-mediated disorders, respectively. Initially, treatment strategies were based on nonspecific inducers of type I IFNs, which were soon replaced by different recombinant proteins...
September 2017: Veterinary Immunology and Immunopathology
https://www.readbyqxmd.com/read/28888122/interferon-%C3%AF-induced-gene-expression-in-bovine-hepatocytes-during-early-pregnancy
#15
B Ruhmann, K Giller, A K Hankele, S E Ulbrich, M Schmicke
Interferon-tau (IFNτ) is the conceptus derived specific early pregnancy signal in bovidae. Locally, IFNτ induces an IFNτ specific gene expression (ISG) in endometrial cells and by this it averts luteolysis of the corpus luteum (CL) by suppressing prostaglandin production. Moreover, it was shown that IFNτ also induces ISG in the liver in pregnant sheep and in liver biopsies from Holstein Friesian heifers on Day 18 of pregnancy. The objective of the present study was to confirm increased hepatic ISG in vivo on Day 18 of pregnancy and to prove if hepatocytes and not non-parenchymal cells react to IFNτ by using immunohistochemistry and primary bovine hepatocytes stimulated in vitro with recombinant bovine IFNτ...
July 31, 2017: Theriogenology
https://www.readbyqxmd.com/read/28882807/atlantic-salmon-endothelial-cells-from-the-heart-were-more-susceptible-than-fibroblasts-from-the-bulbus-arteriosus-to-four-rna-viruses-but-protected-from-two-viruses-by-dsrna-pretreatment
#16
Phuc H Pham, Winnie W L Tong, Ehab Misk, Ginny Jones, John S Lumsden, Niels C Bols
Heart diseases caused by viruses are major causes of Atlantic salmon aquaculture loss. Two Atlantic salmon cardiovascular cell lines, an endothelial cell line (ASHe) from the heart and a fibroblast cell line (BAASf) from the bulbus arteriosus, were evaluated for their response to four fish viruses, CSV, IPNV, VHSV IVa and VHSV IVb, and the innate immune agonist, double-stranded RNA mimic poly IC. All four viruses caused cytopathic effects in ASHe and BAASf. However, ASHe was more susceptible to all four viruses than BAASf...
September 4, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28882802/identification-of-the-functional-regions-of-the-viral-haemorrhagic-septicaemia-virus-vhsv-nv-protein-variants-that-improve-function
#17
Blanca Chinchilla, Eduardo Gomez-Casado
Non-virion (NV) protein is essential for an efficient replication increasing the pathogenicity of the Salmonid novirhabdovirus (formerly IHNV), Piscine novirhabdovirus (formerly VHSV), and Hirame novirhabdovirus (HIRV). The interferon system, apoptosis, and other immune-related genes are modulated by NV to finally induce a deficient antiviral state in the cell. However, little is known about the VHSV NV regions involved in function and location. Here, eight different NV 07.71 fragments and eleven NV 07.71 mutants derived from the region between the two first α-helices have been studied in order to establish the mx and il8 transcript levels in ZF4 cells and the subcellular location...
September 5, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/28862636/wifip-a-web-based-user-interface-for-automated-synchrotron-beamlines
#18
Yoann Sallaz-Damaz, Jean Luc Ferrer
The beamline control software, through the associated graphical user interface (GUI), is the user access point to the experiment, interacting with synchrotron beamline components and providing automated routines. FIP, the French beamline for the Investigation of Proteins, is a highly automatized macromolecular crystallography (MX) beamline at the European Synchrotron Radiation Facility. On such a beamline, a significant number of users choose to control their experiment remotely. This is often performed with a limited bandwidth and from a large choice of computers and operating systems...
September 1, 2017: Journal of Synchrotron Radiation
https://www.readbyqxmd.com/read/28852929/n-pro-his49-and-e-rns-lys412-mutations-in-pig-bovine-viral-diarrhea-virus-type-2-synergistically-enhance-the-cellular-antiviral-response
#19
Jie Tao, Benqiang Li, Jinghua Chen, Chunling Zhang, Yufei Ma, Guoqiang Zhu, Huili Liu
Type I interferons are major components of the innate immune response of hosts, and accordingly, many viruses have evolved mechanisms to modulate the host response during infection. Bovine viral diarrhea virus (BVDV) nonstructural protein N(pro) and structural protein E(rns) play important roles in inhibiting type I interferon. The aim of this study was to explore the epistatic effects of amino acid mutations in N(pro) and E(rns) in porcine ST cells to characterize the immune response induced by BVDV-2. Plasmids with mutant amino acids His49 (H49), Glu22 (E22) in N(pro), and His300 (H300), Lys412 (K412) in E(rns) which had been changed to Alanine (A) had similar effects on type I interferon production in MDBK and ST cells, but resulted in much greater ISG15, OAS, and Mx production in ST cells...
August 29, 2017: Virus Genes
https://www.readbyqxmd.com/read/28764542/mini-beam-modes-on-standard-mx-beamline-bl17u-at-ssrf
#20
Qisheng Wang, Feng Yu, Ying Cui, Kunhao Zhang, Qiangyan Pan, Changyou Zhong, Ke Liu, Huan Zhou, Bo Sun, Jianhua He
The macromolecular crystallography beamlines at third-generation synchrotron facilities play a central role in solving macromolecular crystal structures and also in understanding the biological function at molecular levels. The MX beamline BL17U at Shanghai Synchrotron Radiation Facility is a typical standard MX beamline with a focused beam size (H × V) of FWHM around 80 μm × 45 μm. However the protein samples brought to the beamline are down to 5-10 m from the important and challenging science project now...
July 2017: Review of Scientific Instruments
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