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https://www.readbyqxmd.com/read/29650477/determination-of-24-personal-care-products-in-fish-bile-using-hybrid-solvent-precipitation-and-dispersive-solid-phase-extraction-cleanup-with-ultrahigh-performance-liquid-chromatography-tandem-mass-spectrometry-and-gas-chromatography-mass-spectrometry
#1
Li Yao, Yin-Zhi Lv, Li-Juan Zhang, Wang-Rong Liu, Jian-Liang Zhao, You-Sheng Liu, Qian-Qian Zhang, Guang-Guo Ying
Personal care products (PCPs) are ubiquitous in aquatic environments owing to the continuous discharge of domestic wastewater from highly urbanized regions. These PCPs can be adsorbed by fish and thereafter usually enter the bile of the fish through biliary excretion. In this study, a sensitive method based on a combination of hybrid solvent precipitation and dispersive solid phase extraction (d-SPE) purification was developed to simultaneously extract and detect 24 PCPs, namely, 16 biocides, 4 synthetic musks, and 4 benzotriazoles, from fish bile...
April 5, 2018: Journal of Chromatography. A
https://www.readbyqxmd.com/read/29587792/major-contribution-of-the-rna-binding-domain-of-ns1-in-the-pathogenicity-and-replication-potential-of-an-avian-h7n1-influenza-virus-in-chickens
#2
Sascha Trapp, Denis Soubieux, Alexandra Lidove, Evelyne Esnault, Adrien Lion, Vanaique Guillory, Alan Wacquiez, Emmanuel Kut, Pascale Quéré, Thibaut Larcher, Mireille Ledevin, Virginie Nadan, Christelle Camus-Bouclainville, Daniel Marc
BACKGROUND: Non-structural protein NS1 of influenza A viruses harbours several determinants of pathogenicity and host-range. However it is still unclear to what extent each of its two structured domains (i.e. RNA-binding domain, RBD, and effector domain, ED) contribute to its various activities. METHODS: To evaluate the respective contributions of the two domains, we genetically engineered two variants of an H7N1 low pathogenicity avian influenza virus harbouring amino-acid substitutions that impair the functionality of either domain...
March 27, 2018: Virology Journal
https://www.readbyqxmd.com/read/29570417/reduction-of-marek-s-disease-virus-infection-by-toll-like-receptor-ligands-in-chicken-embryo-fibroblast-cells
#3
Jegarubee Bavananthasivam, Raveendra R Kulkarni, Leah Read, Shayan Sharif
Evolutionarily conserved pattern recognition receptors, including Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns (PAMPs) that are present in microbes. PAMPs induce several pathways downstream of TLRs that lead to induction of antiviral responses. The objective of this study was to investigate the stimulatory effect of various PAMPs (in the form of TLR ligands) in reducing Marek's disease virus (MDV) infection in chicken embryo fibroblast cells (CEFs). To this end, CEFs were pretreated with Pam3CSK4, Poly(IC), lipopolysaccharide (LPS), and CpG ODN as TLR2, TLR3, TLR4, and TLR21 ligands, respectively for 24 h followed by infection with MDV...
March 23, 2018: Viral Immunology
https://www.readbyqxmd.com/read/29560117/hypoxic-marker-ca-ix-and-adhesion-mediator-%C3%AE-catenin-are-downregulated-by-lymphocytic-choriomeningitis-virus-persistent-infection
#4
Andrea Fabianova, Monika Barathova, Lucia Csaderova, Veronika Simko, Miriam Zatovicova, Martina Labudova, Jaromir Pastorek
Renal cell carcinoma is one of the most frequent cancer diseases with high resistance to radio- and chemotherapy. Mutation of VHL gene is frequent in these tumors leading to simulation of hypoxic conditions. Lymphocytic choriomeningitis virus, belonging to RNA viruses, is a neglected human pathogen and teratogen. We have found that infection of renal cell carcinoma cells by lymphocytic choriomeningitis virus strain MX causes a decrease of carbonic anhydrase IX protein and RNA level. Lower expression of carbonic anhydrase IX on the cell surface provides less target for carbonic anhydrase IX-targeted immunotherapy...
February 27, 2018: Oncotarget
https://www.readbyqxmd.com/read/29533241/where-is-crystallography-going
#5
Jonathan M Grimes, David R Hall, Alun W Ashton, Gwyndaf Evans, Robin L Owen, Armin Wagner, Katherine E McAuley, Frank von Delft, Allen M Orville, Thomas Sorensen, Martin A Walsh, Helen M Ginn, David I Stuart
Macromolecular crystallography (MX) has been a motor for biology for over half a century and this continues apace. A series of revolutions, including the production of recombinant proteins and cryo-crystallography, have meant that MX has repeatedly reinvented itself to dramatically increase its reach. Over the last 30 years synchrotron radiation has nucleated a succession of advances, ranging from detectors to optics and automation. These advances, in turn, open up opportunities. For instance, a further order of magnitude could perhaps be gained in signal to noise for general synchrotron experiments...
February 1, 2018: Acta Crystallographica. Section D, Structural Biology
https://www.readbyqxmd.com/read/29530830/transcriptome-analysis-of-olive-flounder-paralichthys-olivaceus-head-kidney-infected-with-moderate-and-high-virulent-strains-of-infectious-viral-hemorrhagic-septicaemia-virus-vhsv
#6
Jee Youn Hwang, Kesavan Markkandan, Mun Gyeong Kwon, Jung Soo Seo, Seung-Il Yoo, Seong Don Hwang, Maeng-Hyun Son, Junhyung Park
Olive flounder (Paralichthys olivaceus) is one of the most valuable marine aquatic species in South Korea and faces tremendous exposure to the viral hemorrhagic septicemia virus (VHSV). Given the growing importance of flounder, it is therefore essential to understand the host defense of P. olivaceus against VHSV infection, but studies on its immune mechanism are hindered by the lack of genomic resources. In this study, the P. olivaceus was infected with disease-causing VHSV isolates, ADC-VHS2012-11 and ADC-VHS2014-5 which showed moderate virulent (20% mortality) and high virulent (65% mortality), in order to investigate the effect of difference in pathogenicity in head kidney during 1, 3, 7 days of post-infection using Illumina sequencing...
March 9, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29518481/epidermal-growth-factor-receptor-egfr-inhibitor-pd153035-reverses-abcg2-mediated-multidrug-resistance-in-non-small-cell-lung-cancer-in-vitro-and-in-vivo
#7
Guan-Nan Zhang, Yun-Kai Zhang, Yi-Jun Wang, Pranav Gupta, Charles R Ashby, Saeed Alqahtani, Tongjin Deng, Susan E Bates, Amal Kaddoumi, John N D Wurpel, Yi-Xiong Lei, Zhe-Sheng Chen
One of the major mediators of multidrug resistance (MDR) in non-small cell lung cancer (NSCLC) is the overexpression of ATP-binding cassette subfamily G member 2 (ABCG2). In this study, we conducted in vitro and in vivo experiments to determine whether PD153035, an inhibitor of EGFR, could reverse ABCG2-mediated MDR in human NSCLC and transfected cells overexpressing ABCG2. The efficacy of SN-38, topotecan, and mitoxantrone (MX) was significantly increased by PD153035, PD153035 significantly reversed ABCG2-mediated MDR by attenuating the efflux activity of this transporter...
March 5, 2018: Cancer Letters
https://www.readbyqxmd.com/read/29510252/antiviral-activity-of-transiently-expressed-mitochondrial-antiviral-signaling-adapter-mavs-orthologue-from-asian-seabass
#8
Rahul Krishnan, Jeena K, Zahoor Mushtaq, K U Shyam, Pani Prasad Kurcheti
The innate immune signaling adapter, Mitochondrial antiviral signaling protein (MAVS) coordinates the signals received from two independent RLRs (RIG-1 and MDA5) to induce IFN & interferon stimulatory genes (ISGs). In the present study, we report identification of an orthologue of MAVS from Lates calcarifer (LcMAVS) and its functional role in piscine RLR signaling. The LcMAVS-cDNA was cloned into pcDNA and transfected into SISS cells. LcMAVS was detected to be a 61KDa protein in western blot. Confocal microscopy demonstrated the mitochondrial localization of LcMAVS...
March 3, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29502306/antiviral-effects-and-mechanisms-of-yinhuapinggan-granule-against-h1n1-influenza-virus-infection-in-raw264-7-cells
#9
Hai-Xia Du, Hui-Fen Zhou, Hao-Fang Wan, Jie-Hong Yang, Yi-Yu Lu, Yu He, Hai-Tong Wan
Yinhuapinggan granule (YHPG), a modified prescription based on Ma-Huang-Tang (MHT), is used in traditional Chinese medicine (TCM) to treat influenza, cough, and viral pneumonia. In this study, we investigated the antiviral effects of YHPG by means of pre-, post-, and co-treatment, and its underlying mechanisms on regulating the levels of inflammatory-related cytokines, modulating the mRNA expressions of interferon-stimulated genes in influenza virus-infected murine macrophage cells (RAW264.7), and evaluating the protein expressions of key effectors in the Type I IFN and pattern recognition receptor (PRRs) signaling pathways...
March 3, 2018: Inflammopharmacology
https://www.readbyqxmd.com/read/29422897/fowl-adenovirus-serotype-4-sd0828-infections-causes-high-mortality-rate-and-cytokine-levels-in-specific-pathogen-free-chickens-compared-to-ducks
#10
Rong Li, Gen Li, Jing Lin, Shaojie Han, Xiaolan Hou, Hongyu Weng, Mengjiao Guo, Zhong Lu, Ning Li, Yingli Shang, Tongjie Chai, Liangmeng Wei
Hydropericardium syndrome and inclusion body hepatitis, together called hydropericardium-hepatitis syndrome, are acute infectious diseases found in chickens. These diseases are caused primarily by fowl adenovirus serotype 4 (FAdV-4) strains. In this study, we isolated a FAdV-4 strain (SD0828) from clinically diseased chickens and phylogenetically analyzed the L1 loops of the hexon protein sequences in 3-week-old specific pathogen-free chickens and ducks infected intramuscularly and orally, determining differences in the pathogenicity by observing clinical signs and gross and histological lesions...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29413814/interferon-induced-mx-protein-from-indian-snow-trout-schizothorax-richardsonii-gray-lacks-critical-functional-features-unlike-its-mammalian-homologues
#11
Ankur Saxena, Kiran Belwal, Ankita Chauhan, Amit Pande
Viral attack within host cells triggers the production of type I interferons and leads to the induction of interferon stimulated genes (ISGs). One of the ISG Mx, encodes type I interferon inducible GTPase that is responsible for the establishment of an anti-viral state within cells. Intriguingly, several isoforms of Mx have been reported in fish, but the structural analysis of fish Mx proteins remains unexplored. For the first time, we have identified and unraveled the molecular structure of Mx protein from Indian snow trout, Schizothorax richardsonii (Gray) a Coldwater fish that inhabits the water bodies in the sub-Himalayan region...
January 31, 2018: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/29407574/pathogenicity-of-duck-hepatitis-a-virus-type-3-and-innate-immune-responses-of-the-ducklings-to-virulent-dhav-3
#12
Xuelian Zhang, Chong Cao, Zhehui Qu, Wenjing Zhang, Yue Liu, Haihui Qi, Chunxue Hao, Wenlong Zhang, Mingchun Gao, Junwei Wang, Bo Ma
Duck virus hepatitis caused by duck hepatitis A virus (DHAV) is an acute and contagious disease. To better understand the pathogenic mechanism of DHAV-3 in ducklings, an infection experiment was performed. Our results showed that typical symptoms were observed in the infected ducklings. DHAV-3 could infect many tissues, leading to pathological lesions, especially on the livers and spleen, and the host immune responses are activated in infection. Real-time quantitative PCR demonstrated that expression of many innate immune-related genes was mostly up-regulated in the livers and spleen, and antiviral innate immune response was established, but not sufficient to restrict the virus replication of lethal dose...
January 26, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29399125/identification-of-potentially-relevant-genes-for-myocardial-infarction-using-rna-sequencing-data-analysis
#13
Qiang Zhao, Ke Wu, Nannan Li, Zhengmei Li, Fenglin Jin
Myocardial infarction (MI) is a heart disease with high morbidity and mortality rates, thus it is critical to identify genes that serve roles during its pathogenesis. The objective of the present study was to identify potentially relevant genes during the progression of the disease. Blood samples from patients with MI and normal controls (n=3/group) were obtained, the RNA was extracted and cDNA libraries were established. RNA sequencing (RNA-seq) was performed on a HiSeq 2500 platform and fragments per kilobase of exon per million fragments mapped was utilized to calculate the gene expression value following preprocessing of the RNA-seq data...
February 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29343573/porcine-mx1-protein-inhibits-classical-swine-fever-virus-replication-by-targeting-nonstructural-protein-ns5b
#14
Jing Zhou, Jing Chen, Xiao-Min Zhang, Zhi-Can Gao, Chun-Chun Liu, Yun-Na Zhang, Jin-Xiu Hou, Zhao-Yao Li, Lin Kan, Wen-Liang Li, Bin Zhou
Mx proteins are interferon-induced GTPases that have broad antiviral activity against a wide range of RNA and DNA viruses; they belong to the dynamin superfamily of large GTPases. In this study, we confirmed the anti-CSFV activity of porcine Mx1 in vitro and showed that porcine Mx2 (poMx2), human MxA (huMxA), and mouse Mx1 (mmMx1) also have anti-CSFV activity in vitro. siRNA experiments revealed that depletion of endogenous poMx1 or poMx2 enhanced CSFV replication, suggesting that porcine Mx proteins are responsible for the antiviral activity of interferon-α (IFNα) against CSFV infection...
January 17, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29325821/dna-vaccine-encoding-myristoylated-membrane-protein-mmp-of-rock-bream-iridovirus-rbiv-induces-protective-immunity-in-rock-bream-oplegnathus-fasciatus
#15
Myung-Hwa Jung, Chamilani Nikapitiya, Sung-Ju Jung
Rock bream iridovirus (RBIV) causes severe mass mortalities in rock bream (Oplegnathus fasciatus) in Korea. In this study, we investigated the potential of viral membrane protein to induce antiviral status protecting rock bream against RBIV infection. We found that fish administered with ORF008L (myristoylated membrane protein, MMP) vaccine exhibited significantly higher levels of survival compared to ORF007L (major capsid protein, MCP). Moreover, ORF008L-based DNA vaccinated fish showed significant protection at 4 and 8 weeks post vaccination (wpv) than non-vaccinated fish after infected with RBIV (6...
February 1, 2018: Vaccine
https://www.readbyqxmd.com/read/29286612/unexpected-lower-expression-of-oncoprotein-gankyrin-in-drug-resistant-abcg2-overexpressing-breast-cancer-cell-lines
#16
Taheri Taheri, Khadijeh Jamialahmadi, Fatemeh Khadijeh
Background: Development of a multidrug resistance (MDR) phenotype to chemotherapy remains a major barrier in the treatment of cancer. Gankyrin (p28, p28GANK or PSMD10) is an oncoprotein overexpressed in different carcinoma cell lines. The aim of this study was to compare Gankyrin expression level in MDR cells (MCF-7/ADR and MCF-7/ MX) and non-MDR counterparts (MCF-7). Methods: Gankyrin, MDR1 (also known as ABCB1; the ATP-binding cassette sub-family B member 1) and ABCG2 (also known as BCRP; the human breast cancer resistance protein) mRNA levels were analyzed by real-time RT-PCR...
December 29, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/29278743/expression-and-purification-of-biologically-active-bovine-interferon-%C3%AE-3-il28b-in-pichia-pastoris
#17
S Barathiraja, P A V Gangadhara, V Umapathi, H J Dechamma, G R Reddy
Interferon lambda-3 (IFNλ3) which is also known as IL28B is a member of type III Interferons which are structurally and genetically different from type I Interferons. These Interferons induce signal transduction pathways similar to type I Interferons which results in the activation of Interferon Stimulated Genes (ISGs). This group of Interferons are tissue specific and reported to have antiviral activity. In the present communication, we report the expression of bovine IFNλ3 gene (coding for the mature protein) in Pichia pastoris, purification of the expressed protein and evaluation of its biological activity...
May 2018: Protein Expression and Purification
https://www.readbyqxmd.com/read/29274924/vp2-pta-motif-encoding-dna-vaccine-confers-protection-against-lethal-challenge-with-infectious-pancreatic-necrosis-virus-ipnv-in-trout
#18
Sohrab Ahmadivand, Mehdi Soltani, Mahdi Behdani, Øystein Evensen, Ehsan Alirahimi, Elahe Soltani, Reza Hassanzadeh, Javad Ashrafi-Helan
IPNV in Atlantic salmon is represented by various strains with different virulence and immunogenicity linked to various motifs of the VP2 capsid. IPNV variant with P217 , T221 , A247 (PTA) motif is found to be avirulent in Atlantic salmon, but virulent in rainbow trout, and other salmonid species. This study describes a DNA vaccine delivered intramuscularly encoding the VP2 protein of infectious pancreatic necrosis virus (IPNV) with PTA motif that confers high protection in rainbow trout (Oncorhynchus mykiss)...
February 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29207185/novel-multi%C3%A2-kinase-inhibitor-t03-inhibits-taxol%C3%A2-resistant-breast-cancer
#19
Yan Li, Chunxia Liu, Ke Tang, Yan Chen, Kang Tian, Zhiqiang Feng, Jindong Chen
Activation of kinase-associated signaling pathways is one of the leading causes of various malignant phenotypes in breast tumors. Strategies of drug discovery and development have investigated approaches to target the inhibition of protein kinase signaling. In the current study, the anti‑tumor activities of a novel multi‑kinase inhibitor, T03 were evaluated in breast cancer. T03 inhibited Taxol‑resistant breast cancer cell proliferation and induced cell cycle arrest and apoptosis in vitro and in vivo...
February 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29017946/cloning-and-expression-of-malabar-grouper-epinephelus-malabaricus-adar1-gene-in-response-to-immune-stimulants-and-nervous-necrosis-virus
#20
Thirunavukkarasu Periyasamy, Joan Tang Xiao Joe, Ming-Wei Lu
ADARs are RNA editing catalysts that bind double-stranded RNA and convert adenosine to inosine, a process that can lead to destabilization of dsRNA structures and suppression of mRNA translation. In mammals, ADAR1 genes are involved in various cellular pathways, including interferon (IFN)-mediated response. However, the function of fish ADAR1 remains unclear. We report here the cloning of ADAR1 in Malabar grouper (Epinephelus malabaricus) (MgADAR1) and its response to various immune stimulants. The MgADAR1 cDNA is 5371-bp long, consisting of an open reading frame encoding a putative protein of 1381 amino acids, a 235-nt 5'-terminal untranslated region (UTR), and a 990-nt 3'-UTR...
October 7, 2017: Fish & Shellfish Immunology
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