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https://www.readbyqxmd.com/read/29322750/use-of-streptococcus-salivarius-k12-to-reduce-the-incidence-pharyngo-tonsillitis-and-acute-otitis-media-in-children-a-retrospective-analysis-in-not-recurrent-pediatric-subjects
#1
Francesco DI Pierro, Paolo Risso, Elena Poggi, Anna Timitilli, Sara Bolloli, Maurizio Bruno, Egidio Caneva, Riccardo Campus, Alessandro Giannattasio
BACKGROUND: Previous trials, performed in subjects affected by recurrent streptococcal pharyngo-tonsillar infection, have shown that the use for 90 days of Streptococcus salivarius K12 (K12), an oral colonizing probiotic producing lantibiotic bacteriocins, reduces the occurrence of streptococcal and viral pharyngitis and acute otitis media (AOM). The aim was to evaluate the role of K12 in reducing the incidence of streptococcal and viral pharyngo-tonsillitis and AOM when administered in two separate trimesters, from October to December and then from April to June, in pediatric subjects with non-recurrent streptococcal infection...
January 11, 2018: Minerva Pediatrica
https://www.readbyqxmd.com/read/29311076/crystal-structure-of-nisi-in-a-lipid-free-form-the-nisin-immunity-protein-from-lactococcus-lactis
#2
Jin Hee Jeong, Sung Chul Ha
Nisin is a lantibiotic, a family of polypeptides containing lanthionine with antimicrobial activity. Nisin-producing microorganisms require immunity proteins for self-protection from nisin itself. Lactococcus lactis, a microorganism that synthesizes nisin, has an integral NisFEG ABC transporter and NisI lipoprotein that function in the nisin immunity. Here, we present the crystal structure of the full length of NisI22-C, a lipid-free form of NisI, determined at 1.9 Å resolution. Similar to the NMR structures of N- and C-terminal domain of NisI, NisI22-C is composed of N- and C-terminal domains, both of which display a fold similar to that found in SpaI, a lipoprotein with immunity against subtilin in Bacillus subtilis The full length structure of NisI22-c reveals a large and deep cleft by the interdomain association, one side of which is occupied by the residues important for immunity...
January 8, 2018: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/29247062/cerr-a-single-domain-regulatory-protein-of-the-luxr-family-promotes-the-cerecidin-production-and-immunity-in-bacillus-cereus
#3
Li Zhang, Kunling Teng, Jian Wang, Zheng Zhang, Jie Zhang, Shutao Sun, Lili Li, Xiaopan Yang, Jin Zhong
Cerecidins are small lantibiotics from Bacillus cereus that were obtained using a semi-in vitro biosynthesis strategy and showed prominent antimicrobial activities against certain Gram-positive bacteria. However, the parental strain B. cereus As 1.1846 is incapable of producing cerecidins, most probably due to the transcriptional repression of the cerecidin gene cluster. Located in the cerecidin gene cluster, cerR encodes a putative response regulator protein that belongs to LuxR-family transcriptional regulators...
December 15, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/29222758/differential-proteomics-based-on-2d-difference-in-gel-electrophoresis-and-tandem-mass-spectrometry-for-the-elucidation-of-biological-processes-in-antibiotic-producer-bacterial-strains
#4
Giuseppe Gallo, Andrea Scaloni
Proteomics based on 2D-Difference In Gel Electrophoresis (2D-DIGE) coupled with mass spectrometry (MS) procedures can be considered a "gold standard" to determine quantitatively and comparatively protein abundances in cell extracts from different biological sources/conditions according to a gel-based approach. In particular, 2D-DIGE is used for protein specie separation, detection, and relative quantification, whenever tandem MS is used to obtain peptide sequence information that is managed according to bioinformatic procedures to identify the differentially represented protein species...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29195488/improving-the-attrition-rate-of-lanthipeptide-discovery-for-commercial-applications
#5
Mengxin Geng, Leif Smith
Lanthipeptides are a class of ribosomally synthesized and post-translationally modified peptides. Lanthipeptides with antimicrobial activity are referred to as lantibiotics. Lantibiotics are generally active against Gram-positive bacteria. However, some modifications have expanded their activity toward Gram-negative bacteria. Furthermore, additional functions aside from antibacterial activities have been reported for lanthipeptides. Areas covered: This review provides a synopsis of current anthipeptide research for potential therapeutics...
December 1, 2017: Expert Opinion on Drug Discovery
https://www.readbyqxmd.com/read/29167920/atpase-activity-regulation-by-leader-peptide-processing-of-abc-transporter-maturation-and-secretion-protein-nukt-for-lantibiotic-nukacin-isk-1
#6
Sen Zheng, Jun-Ichi Nagao, Mami Nishie, Takeshi Zendo, Kenji Sonomoto
Lantibiotic nukacin ISK-1 is produced by Staphylococcus warneri ISK-1. The dual functional transporter NukT, an ABC transporter maturation and secretion protein, contributes to cleavage of the leader peptide from the prepeptide (modified NukA) and the final transport of nukacin ISK-1. NukT consists of an N-terminal peptidase domain (PEP), a C-terminal nucleotide-binding domain (NBD), and a transmembrane domain (TMD). In this study, NukT and its peptidase-inactive mutant were expressed, purified, and reconstituted into liposomes for analysis of their peptidase and ATPase activities...
November 22, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/29164875/insights-into-the-mechanism-of-action-of-the-two-peptide-lantibiotic-lacticin-3147
#7
Alireza Bakhtiary, Stephen A Cochrane, Pascal Mercier, Ryan T McKay, Mark Miskolzie, Clarissa S Sit, John C Vederas
Lacticin 3147 is a two peptide lantibiotc (LtnA1 and LtnA2) that displays nanomolar activity against many Gram-positive bacteria. Lacticin 3147 may exert its antimicrobial effect by several mechanisms. Isothermal titration calorimetry experiments show that only LtnA1 binds to the peptidoglycan precursor lipid II, which could inhibit peptidoglycan biosynthesis. An experimentally supported model of the resulting complex suggests that the key binding partners are the C-terminus of LtnA1 and pyrophosphate of lipid II...
November 22, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29092042/expanding-the-potential-of-nai-107-for-treating-serious-eskape-pathogens-synergistic-combinations-against-gram-negatives-and-bactericidal-activity-against-non-dividing-cells
#8
Cristina Brunati, Thomas T Thomsen, Eleonora Gaspari, Sonia Maffioli, Margherita Sosio, Daniela Jabes, Anders Løbner-Olesen, Stefano Donadio
Objectives: To characterize NAI-107 and related lantibiotics for their in vitro activity against Gram-negative pathogens, alone or in combination with polymyxin, and against non-dividing cells or biofilms of Staphylococcus aureus. NAI-107 was also evaluated for its propensity to select or induce self-resistance in Gram-positive bacteria. Methods: We used MIC determinations and chequerboard experiments to establish the antibacterial activity of the examined compounds against target microorganisms...
October 30, 2017: Journal of Antimicrobial Chemotherapy
https://www.readbyqxmd.com/read/28974812/effect-of-nisin-on-biogenic-amines-and-shelf-life-of-vacuum-packaged-rainbow-trout-oncorhynchus-mykiss-fillets
#9
Clemencia Chaves López, Annalisa Serio, Costanza Montalvo, Cristina Ramirez, José Angel Peréz Álvarez, Antonello Paparella, Dino Mastrocola, Maria Martuscelli
Nisin is a lantibiotic exhibiting antimicrobial activity against a wide range of Gram-positive bacteria, or some Gram-negative bacteria when used in combination with other preservative agents. The objective of the present work was to study the effect of nisin treatment on biogenic amines occurrence and shelf life of refrigerated (4 °C) vacuum packaged rainbow trout samples. For this purpose samples were divided in two batches: the experimental batch (CB-N), consisting of samples immersed in sterilized broth formulated with soy milk 1...
September 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28912758/the-n-terminal-region-of-nisin-is-important-for-the-bceab-type-abc-transporter-nsrfp-from-streptococcus-agalactiae-coh1
#10
Jens Reiners, Marcel Lagedroste, Katja Ehlen, Selina Leusch, Julia Zaschke-Kriesche, Sander H J Smits
Lantibiotics are (methyl)-lanthionine-containing antimicrobial peptides produced by several Gram-positive bacteria. Some human pathogenic bacteria express specific resistance proteins that counteract this antimicrobial activity of lantibiotics. In Streptococcus agalactiae COH1 resistance against the well-known lantibiotic nisin is conferred by, the nisin resistance protein (NSR), a two-component system (NsrRK) and a BceAB-type ATP-binding cassette (ABC) transporter (NsrFP). The present study focuses on elucidating the function of NsrFP via its heterologous expression in Lactococcus lactis...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28869797/identification-of-the-biosynthesis-gene-cluster-for-the-novel-lantibiotic-paenilan-from-paenibacillus-polymyxa-e681-and-characterization-of-its-product
#11
J-E Park, H-R Kim, S-Y Park, S-K Choi, S-H Park
AIMS: To analyse the function of a putative lantibiotic gene cluster of Paenibacillus polymyxa E681 and to characterize its product, paenilan. METHODS AND RESULTS: Comparative analysis of the lantibiotic gene cluster of E681 revealed that the cluster, consisting of 11 open reading frames, is involved in the biosynthesis of a class I lantibiotic. The pnlA gene encoding the prepeptide PnlA was identified and P. polymyxa strain EPT14 producing only paenilan was constructed by knockout of the other five antibiotic biosynthetic gene clusters of E681...
September 4, 2017: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/28867788/high-throughput-identification-of-antimicrobial-peptides-from-fish-gastrointestinal-microbiota
#12
Bo Dong, Yunhai Yi, Lifeng Liang, Qiong Shi
Antimicrobial peptides (AMPs) are a group of small peptides, which are secreted by almost all creatures in nature. They have been explored in therapeutic and agricultural aspects as they are toxic to many bacteria. A considerable amount of work has been conducted in analyzing 16S and metagenomics of the gastrointestinal (GI) microbiome of grass carp (Ctenopharyngodon idellus). However, these datasets are still untapped resources. In this present study, a homologous search was performed to predict AMPs from our newly generated metagenome of grass carp...
August 30, 2017: Toxins
https://www.readbyqxmd.com/read/28857034/discovery-of-a-novel-lantibiotic-nisin-o-from-blautia-obeum-a2-162-isolated-from-the-human-gastrointestinal-tract
#13
Diane Hatziioanou, Cristina Gherghisan-Filip, Gerhard Saalbach, Nikki Horn, Udo Wegmann, Sylvia H Duncan, Harry J Flint, Melinda J Mayer, Arjan Narbad
A novel lanC-like sequence was identified from the dominant human gut bacterium Blautia obeum strain A2-162. This sequence was extended to reveal a putative lantibiotic operon with biosynthetic and transport genes, two sets of regulatory genes, immunity genes, three identical copies of a nisin-like lanA gene with an unusual leader peptide, and a fourth putative lanA gene. Comparison with other nisin clusters showed that the closest relationship was to nisin U. B. obeum A2-162 demonstrated antimicrobial activity against Clostridium perfringens when grown on solid medium in the presence of trypsin...
August 31, 2017: Microbiology
https://www.readbyqxmd.com/read/28827688/the-non-lantibiotic-bacteriocin-garvicin-q-targets-man-pts-in-a-broad-spectrum-of-sensitive-bacterial-genera
#14
Aleksandra Tymoszewska, Dzung B Diep, Paulina Wirtek, Tamara Aleksandrzak-Piekarczyk
Mannose phosphotransferase system (Man-PTS) is the main mannose permease in bacteria but it is also a known receptor for subclass IIa bacteriocins (pediocin-like group) as well as subclass IId lactococcin A (LcnA) and lactococcin B (LcnB) (LcnA-like group). Subclass IIa bacteriocins exhibit a strong activity against Listeria spp. but they are not against Lactococcus spp. In contrast, the LcnA-like bacteriocins act only against Lactococcus lactis strains. Garvicin Q (GarQ) is a subclass IId bacteriocin with minor similarity to LcnA-like bacteriocins and a relatively broad antimicrobial spectrum including, among others, Listeria and Lactococcus spp...
August 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28809560/recombinant-macrocyclic-lanthipeptides-incorporating-non-canonical-amino-acids
#15
Claudio Zambaldo, Xiaozhou Luo, Angad P Mehta, Peter G Schultz
Nisin is a complex lanthipeptide that has broad spectrum antibacterial activity. In efforts to broaden the structural diversity of this ribosomally synthesized lantibiotic, we now report the recombinant expression of Nisin variants that incorporate noncanonical amino acids (ncAAs) at discrete positions. This is achieved by expressing the nisA structural gene, cyclase (nisC) and dehydratase (nisB), together with an orthogonal nonsense suppressor tRNA/aminoacyl-tRNA synthetase pair in Escherichia coli. A number of ncAAs with novel chemical reactivity were genetically incorporated into NisA, including an α-chloroacetamide-containing ncAA that allowed for the expression of Nisin variants with novel macrocyclic topologies...
August 15, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28710266/specificity-of-subtilin-mediated-activation-of-histidine-kinase-spak
#16
Christoph Geiger, Tobias Spieß, Sophie Marianne Korn, Peter Kötter, Karl-Dieter Entian
Autoinduction via two-component systems is a widespread regulatory mechanism that senses environmental and metabolic changes. Although the lantibiotics nisin and subtilin are closely related and share the same lanthionine ring structure, they autoinduce their biosynthesis in a highly specific manner. Subtilin can only activate the two-component system SpaRK of Bacillus subtilis, whereas nisin activates solely the two-component system NisRK of Lactococcus lactis To identify components that determine the specificity of subtilin autoinduction, several variants of the respective lantibiotic were analyzed for their autoinduction capacity...
July 14, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28695849/full-length-nisin-immunity-protein-nisi-from-lactococcus-lactis-in-a-lipid-free-form-crystallization-and-x-ray-analysis
#17
Jin Hee Jeong, Sung Chul Ha
NisI is a lantibiotic-binding lipoprotein that is specific for nisin. Nisin-producing microorganisms use NisI as an immunity protein for self-protection against nisin. Here, the purification, crystallization and preliminary X-ray diffraction of full-length NisI from Lactobacillus lactis in a lipid-free form (NisI22-C) are reported. Importantly, reductive methylation of the lysine residues in NisI22-C was essential for initial crystallization. Only methylated NisI22-C crystallized. The optimized crystals of methylated NisI22-C were grown in 30-40 mM ammonium sulfate, 0...
July 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28675292/substrate-specificity-of-the-secreted-nisin-leader-peptidase-nisp
#18
Marcel Lagedroste, Sander H J Smits, Lutz Schmitt
Nisin (NisA) is an antimicrobial peptide produced by Lactococcus lactis and belongs to the class of lanthipeptides, more specifically to the class of lantibiotics. They are ribosomally synthesized as a precursor peptide and are comprised of an N-terminal leader peptide and a C-terminal core peptide. The core peptide is post-translationally modified and contains dehydrated amino acids in addition to five (methyl)-lanthionine rings, which are crucial for its activity. The leader peptide serves as a signal sequence and ensures that NisA remains inactive but secretion-competent within the cell...
August 1, 2017: Biochemistry
https://www.readbyqxmd.com/read/28659619/nano-engineering-the-antimicrobial-spectrum-of-lantibiotics-activity-of-nisin-against-gram-negative-bacteria
#19
Marija Vukomanović, Vojka Žunič, Špela Kunej, Boštjan Jančar, Samo Jeverica, Rok Podlipec, Danilo Suvorov
Lantibiotics, bacteria-sourced antimicrobial peptides, are very good candidates for effective and safe food additives. Among them, nisin is already approved by the EU and FDA, and has been used in food preservation for the past 40 years. Now, there is a possibility and strong interest to extend its applicability to biomedicine for designing innovative alternatives to antibiotics. The main obstacle is, however, its naturally narrow spectrum of antimicrobial activity, focused on Gram positive bacteria. Here we demonstrate broadening nisin's spectrum to Gram negative bacteria using a nano-engineering approach...
June 28, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28654661/structure-dynamics-and-kinetics-of-two-component-lantibiotic-lichenicidin
#20
Alejandra de Miguel, Rafael Tapia-Rojo, Tillmann Utesch, Maria Andrea Mroginski
Two variants of the two-component Lantibiotic Lichenicidin, produced by the strains B. Licheniformis VK21 and I89 (Lchα/ Lchβ and Bliα/ Bliβ peptides, respectively) have been investigated by means of 2 μs-long all-atom molecular dynamics simulations combined with Markov State Models. This rigorous statistical analysis enabled to evaluate the dynamic and kinetic properties of the aforementioned systems which are not accessible via experimental techniques. The structural flexibility characteristic of these small peptides is understood by a delicate equilibrium between random coil, α-helices and β-sheet structures...
2017: PloS One
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