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https://www.readbyqxmd.com/read/28583849/rapid-detection-of-lactococcuslactis-isolates-producing-the-lantibiotics-nisin-lacticin-481-and-lacticin-3147-using-maldi-tof-ms
#1
Tomás García-Cayuela, Teresa Requena, M Carmen Martínez-Cuesta, Carmen Peláez
The aim of the study was to evaluate the potential use of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS) for fast and reliable detection of strains producing the lantibiotics nisin, lacticin 481 and lacticin 3147 in a large collection of lactococci. A total of one hundred lactococcal isolates from traditional ewe's and goat's raw milk cheeses were identified to the species level as Lactococcuslactis by MALDI-TOF MS based on comparison with lactococcal entries in the BioTyper database...
June 2, 2017: Journal of Microbiological Methods
https://www.readbyqxmd.com/read/28579477/two-highly-divergent-lineages-of-exfoliative-toxin-b-encoding-plasmids-revealed-in-impetigo-strains-of-staphylococcus-aureus
#2
Tibor Botka, Vladislava Růžičková, Karla Svobodová, Roman Pantůček, Petr Petráš, Darina Čejková, Jiří Doškař
Exfoliative toxin B (ETB) encoded by some large plasmids plays a crucial role in epidermolytic diseases caused by Staphylococcus aureus. We have found as yet unknown types of etb gene-positive plasmids isolated from a set of impetigo strains implicated in outbreaks of pemphigus neonatorum in Czech maternity hospitals. Plasmids from the strains of clonal complex CC121 were related to archetypal plasmid pETBTY4. Sharing a 33-kb core sequence including virulence genes for ETB, EDIN C, and lantibiotics, they were assigned to a stand-alone lineage, named pETBTY4-based plasmids...
May 29, 2017: International Journal of Medical Microbiology: IJMM
https://www.readbyqxmd.com/read/28576760/discovery-and-biosynthesis-of-mathermycin-from-marine-derived-marinactinospora-thermotolerans-scsio-00652
#3
Erquan Chen, Qi Chen, Shaoming Chen, Bing Xu, Jianhua Ju, Huan Wang
Lantibiotics are antimicrobial peptides belonging to the family of ribosomally synthesized and post-translationally modified peptides (RiPPs) and featured by the thioether linkages in their structures. In this study, we identified the biosynthetic gene cluster of a cinnamycin analog, named mathermycin, from Marinactinospora thermotolerans SCSIO 00652 and reconstituted its biosynthesis in Streptomyces lividans and E. coli. Key posttranslational modification enzymes of mathermycin were characterized. Mathermycin exhibited antimicrobial activities and therefore representing an example of cinnamycin-like lantibiotics from Marinactinospora species...
June 2, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28500042/carboxyl-analogue-of-mutacin-1140-a-scaffold-for-lead-antibacterial-discovery
#4
Jerome Escano, Akshaya Ravichandran, Bita Salamat, Leif Smith
Mutacin 1140 belongs to the epidermin group of lantibiotics. Epidermin class lantibiotics are ribosomally synthesized and post translationally modified antibiotics with potent activity against Gram-positive bacteria. In particular, this class is effective at targeting drug resistant Streptococcus pneumoniae, MRSA,Mycobacterium tuberculosis, and Clostridium difficile A C-terminal S-[(Z)-2-aminovinyl]-D-cysteine (AviCys) residue is derived from a decarboxylation of a terminal cysteine that is involved in one of the lanthionine ring formation...
May 12, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/28462050/a-profile-hidden-markov-model-to-investigate-the-distribution-and-frequency-of-lanb-encoding-lantibiotic-modification-genes-in-the-human-oral-and-gut-microbiome
#5
Calum J Walsh, Caitriona M Guinane, Paul W O' Toole, Paul D Cotter
BACKGROUND: The human microbiota plays a key role in health and disease, and bacteriocins, which are small, bacterially produced, antimicrobial peptides, are likely to have an important function in the stability and dynamics of this community. Here we examined the density and distribution of the subclass I lantibiotic modification protein, LanB, in human oral and stool microbiome datasets using a specially constructed profile Hidden Markov Model (HMM). METHODS: The model was validated by correctly identifying known lanB genes in the genomes of known bacteriocin producers more effectively than other methods, while being sensitive enough to differentiate between different subclasses of lantibiotic modification proteins...
2017: PeerJ
https://www.readbyqxmd.com/read/28362216/strategies-for-combating-bacterial-biofilms-a-focus-on-anti-biofilm-agents-and-their-mechanisms-of-action
#6
Ranita Roy, Monalisa Tiwari, Gianfranco Donelli, Vishvanath Tiwari
Biofilm refers to the complex, sessile communities of microbes found either attached to a surface or buried firmly in an extracellular matrix as aggregates. The biofilm matrix surrounding bacteria makes them tolerant to harsh conditions and resistant to antibacterial treatments. Moreover, the biofilms are responsible for causing a broad range of chronic diseases and due to the emergence of antibiotic resistance in bacteria it has really become difficult to treat them with efficacy. Furthermore, the antibiotics available till date are ineffective for treating these biofilm related infections due to their higher values of minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC), which may result in in-vivo toxicity...
March 31, 2017: Virulence
https://www.readbyqxmd.com/read/28352561/synergistic-effect-of-haloduracin-and-chloramphenicol-against-clinically-important-gram-positive-bacteria
#7
Abolghasem Danesh, Åsa Ljungh, Bo Mattiasson, Gashaw Mamo
The emergence of drug-resistant pathogens has triggered the search for more efficient antimicrobial agents and formulations for treatment of infections. In recent years, combination therapy has become one of the effective clinical practices in treating infections. The present study deals with the effect of haloduracin, a lantibiotic bateriocin and chloramphenicol against clinically important bacteria. The combined use of haloduracin and chloramphenicol resulted in remarkable synergy against a spectrum of microorganisms including strains of Staphylococcus aureus, Enterococcus faecium, Enterococcus faecalis and different groups of Streptococcus...
March 2017: Biotechnology Reports
https://www.readbyqxmd.com/read/28306363/genome-database-mining-for-the-discovery-of-novel-lantibiotics
#8
REVIEW
Stephanie K Sandiford
The effectiveness of lantibiotics against MDR pathogens and the progression of agents MU1140, NAI-107, NVB302 and duramycin into pre-clinical and clinical trials have highlighted their potential in the fight against bacterial resistance. The number of known lantibiotics and knowledge of their biosynthetic pathways has increased in recent years due to higher quality genomic data being delivered by next generation sequencing technologies combined with the development of specific genome mining tools, enabling the prediction of lantibiotic clusters...
May 2017: Expert Opinion on Drug Discovery
https://www.readbyqxmd.com/read/28294058/recognizing-and-predicting-thioether-bridges-formed-by-lanthionine-and-%C3%AE-methyllanthionine-in-lantibiotics-using-a-random-forest-approach-with-feature-selection
#9
ShaoPeng Wang, Yu-Hang Zhang, Ning Zhang, Lei Chen, Tao Huang, Yu-Dong Cai
Lantibiotics, which are usually produced from Gram-positive bacteria, are regarded as one type of special bacteriocins. Lantibiotics have unsaturated amino acid residues formed by lanthionine (Lan) and β-methyllanthionine (MeLan) residues as a ring structure in the peptide. They are derived from the serine and threonine residues and are essential to preventing the growth of other similar strains. In this pioneering work, we firstly proposed a machine learning method to recognize and predict the Lan and MeLan residues in the protein sequences of lantibiotics...
March 10, 2017: Combinatorial Chemistry & High Throughput Screening
https://www.readbyqxmd.com/read/28285660/genetic-and-biochemical-characterization-of-hyicin-3682-the-first-bacteriocin-reported-for-staphylococcus-hyicus
#10
Patrícia Carlin Fagundes, Ilana Nascimento de Sousa Santos, Márcia Silva Francisco, Rodolpho Mattos Albano, Maria do Carmo de Freire Bastos
Hyicin 3682, the first bacteriocin reported for Staphylococcus hyicus, is a BsaCOL variant produced by S. hyicus 3682, a strain isolated from bovine milk. Hyicin 3682 is found in the culture supernatant, is bactericidal and its producing strain exhibits a much broader spectrum of antimicrobial activity than the producing strain of BsaCOL against several Gram-positive bacteria, which include foodborne pathogens, food-spoilage microorganisms and bacterial species of medical and veterinary importance. Sequencing of the genome of S...
May 2017: Microbiological Research
https://www.readbyqxmd.com/read/28270262/lantibiotics-produced-by-actinobacteria-and-their-potential-applications-a-review
#11
Karen Machado Gomes, Rafael Silva Duarte, Maria do Carmo de Freire Bastos
The phylum Actinobacteria, which comprises a great variety of Gram-positive bacteria with a high G+C content in their genomes, is known for its large production of bioactive compounds, including those with antimicrobial activity. Among the antimicrobials, bacteriocins, ribosomally synthesized peptides, represent an important arsenal of potential new drugs to face the increasing prevalence of resistance to antibiotics among microbial pathogens. The actinobacterial bacteriocins form a heterogeneous group of substances that is difficult to adapt to most proposed classification schemes...
February 2017: Microbiology
https://www.readbyqxmd.com/read/28235608/the-complete-genome-sequence-of-bacillus-velezensis-9912d-reveals-its-biocontrol-mechanism-as-a-novel-commercial-biological-fungicide-agent
#12
Hua-Qi Pan, Qing-Lian Li, Jiang-Chun Hu
A Bacillus sp. 9912 mutant, 9912D, was approved as a new biological fungicide agent by the Ministry of Agriculture of the People's Republic of China in 2016 owing to its excellent inhibitory effect on various plant pathogens and being environment-friendly. Here, we present the genome of 9912D with a circular chromosome having 4436 coding DNA sequences (CDSs), and a circular plasmid encoding 59 CDSs. This strain was finally designated as Bacillus velezensis based on phylogenomic analyses. Genome analysis revealed a total of 19 candidate gene clusters involved in secondary metabolite biosynthesis, including potential new type II lantibiotics...
April 10, 2017: Journal of Biotechnology
https://www.readbyqxmd.com/read/28184221/identification-of-key-residues-in-the-nisk-sensor-region-for-nisin-biosynthesis-regulation
#13
Xiaoxuan Ge, Kunling Teng, Jian Wang, Fangyuan Zhao, Jie Zhang, Jin Zhong
Histidine kinase (HK) NisK is well known to sense lantibiotic nisin for regulating the biosynthesis of nisin. NisK possesses two trans-membrane segments and a large extracellular region and nisin contains 34 amino acids with five lanthionine rings. Unlike most peptide sensing HK with multi trans-membrane segments, NisK is a representative of a group of rarely reported HK that sense peptide as ligand. To reveal how NisK senses nisin molecule to regulate nisin biosynthesis, we constructed a reporter Lactococcus lactis strain with nisRK constitutively expressed and a reporter gene lacZ expressed under the control of promoter P nisA ...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28169337/stoichiometry-and-structure-of-a-lantibiotic-maturation-complex
#14
Jens Reiners, André Abts, Rebecca Clemens, Sander H J Smits, Lutz Schmitt
Lantibiotics are ribosomally synthesized antimicrobial peptides secreted by mainly Gram-positive bacteria. Class 1 lantibiotics mature via two modification steps introduced by a modification LanBC complex. For the lantibiotic nisin, the dehydratase NisB catalyzes the dehydration of serine and threonine residues in the so-called core peptide. Second, five (methyl)-lanthionine rings are introduced in a regio- and stereospecific manner by the cyclase NisC. Here, we characterized the assembly of the NisBC complex in vitro, which is only formed in the presence of the substrate...
February 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28161626/turning-hydrophilic-bacteria-into-biorenewable-hydrophobic-material-with-potential-antimicrobial-activity-via-interaction-with-chitosan
#15
Orakan Hanpanich, Pravit Wongkongkatep, Thunyarat Pongtharangkul, Jirarut Wongkongkatep
Alteration of a bacteriocin-producing hydrophilic bacterium, Lactococcus lactis IO-1, into a hydrophobic material with potential antimicrobial activity using chitosan was investigated and compared with five other bacterial species with industrial importance. The negatively charged bacterial cells were neutralized by positively charged chitosan, resulting in a significant increase in the hydrophobicity of the bacterial cell surface. The largest Gram-positive B. megaterium ATCC 14581 showed a moderate response to chitosan while the smaller E...
April 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28135077/mechanistic-understanding-of-lanthipeptide-biosynthetic-enzymes
#16
Lindsay M Repka, Jonathan R Chekan, Satish K Nair, Wilfred A van der Donk
Lanthipeptides are ribosomally synthesized and post-translationally modified peptides (RiPPs) that display a wide variety of biological activities, from antimicrobial to antiallodynic. Lanthipeptides that display antimicrobial activity are called lantibiotics. The post-translational modification reactions of lanthipeptides include dehydration of Ser and Thr residues to dehydroalanine and dehydrobutyrine, a transformation that is carried out in three unique ways in different classes of lanthipeptides. In a cyclization process, Cys residues then attack the dehydrated residues to generate the lanthionine and methyllanthionine thioether cross-linked amino acids from which lanthipeptides derive their name...
April 26, 2017: Chemical Reviews
https://www.readbyqxmd.com/read/28032983/two-flavoenzymes-catalyze-the-post-translational-generation-of-5-chlorotryptophan-and-2-aminovinyl-cysteine-during-nai-107-biosynthesis
#17
Manuel A Ortega, Dillon P Cogan, Subha Mukherjee, Neha Garg, Bo Li, Gabrielle N Thibodeaux, Sonia I Maffioli, Stefano Donadio, Margherita Sosio, Jerome Escano, Leif Smith, Satish K Nair, Wilfred A van der Donk
Lantibiotics are ribosomally synthesized and post-translationally modified antimicrobial peptides containing thioether rings. In addition to these cross-links, the clinical candidate lantibiotic NAI-107 also possesses a C-terminal S-[(Z)-2-aminovinyl]-d-cysteine (AviCys) and a unique 5-chloro-l-tryptophan (ClTrp) moiety linked to its potent bioactivity. Bioinformatic and genetic analyses on the NAI-107 biosynthetic gene cluster identified mibH and mibD as genes encoding flavoenzymes responsible for the formation of ClTrp and AviCys, respectively...
February 17, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/27914001/subtilosin-prevents-biofilm-formation-by-inhibiting-bacterial-quorum-sensing
#18
Ammar Algburi, Saskia Zehm, Victoria Netrebov, Anzhelica B Bren, Vladimir Chistyakov, Michael L Chikindas
Subtilosin, the cyclic lantibiotic protein produced by Bacillus subtilis KATMIRA1933, targets the surface receptor and electrostatically binds to the bacterial cell membrane. In this study, subtilosin was purified using ammonium sulfate ((NH4)2SO4) precipitation and purified via column chromatography. Subtilosin's antibacterial minimum and sub-minimum inhibitory concentrations (MIC and sub-MIC) and anti-biofilm activity (biofilm prevention) were established. Subtilosin was evaluated as a quorum sensing (QS) inhibitor in Gram-positive bacteria using Fe(III) reduction assay...
December 2, 2016: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/27902417/lantibiotics-produced-by-actinobacteria-and-their-potential-applications-a-review
#19
Karen Gomes, Rafael Silva Duarte, Maria do Carmo de Freire Bastos
The phylum Actinobacteria, which comprises a great variety of Gram-positive bacteria with a high G+C content in their genomes, is known for its large production of bioactive compounds, including those with antimicrobial activity. Among the antimicrobials, bacteriocins, ribosomally-synthesized peptides, represent an important arsenal of potential new drugs to face the increasing prevalence of resistance to antibiotics among microbial pathogens. The actinobacterial bacteriocins form a heterogeneous group of substances that is difficult to adapt to most proposed classification schemes...
November 22, 2016: Microbiology
https://www.readbyqxmd.com/read/27864176/insights-into-the-biosynthesis-of-duramycin
#20
Liujie Huo, Ayşe Ökesli, Ming Zhao, Wilfred A van der Donk
Lantibiotics are ribosomally synthesized and posttranslationally modified antimicrobial peptides that are characterized by the thioether cross-linked bisamino acids lanthionine (Lan) and methyllanthionine (MeLan). Duramycin contains 19 amino acids, including one Lan and two MeLans, an unusual lysinoalanine (Lal) bridge formed from the ε-amino group of lysine 19 and a serine residue at position 6, and an erythro-3-hydroxy-l-aspartic acid at position 15. These modifications are important for the interactions of duramycin with its biological target, phosphatidylethanolamine (PE)...
February 1, 2017: Applied and Environmental Microbiology
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