keyword
MENU ▼
Read by QxMD icon Read
search

Lantibiotics

keyword
https://www.readbyqxmd.com/read/28184221/identification-of-key-residues-in-the-nisk-sensor-region-for-nisin-biosynthesis-regulation
#1
Xiaoxuan Ge, Kunling Teng, Jian Wang, Fangyuan Zhao, Jie Zhang, Jin Zhong
Histidine kinase (HK) NisK is well known to sense lantibiotic nisin for regulating the biosynthesis of nisin. NisK possesses two trans-membrane segments and a large extracellular region and nisin contains 34 amino acids with five lanthionine rings. Unlike most peptide sensing HK with multi trans-membrane segments, NisK is a representative of a group of rarely reported HK that sense peptide as ligand. To reveal how NisK senses nisin molecule to regulate nisin biosynthesis, we constructed a reporter Lactococcus lactis strain with nisRK constitutively expressed and a reporter gene lacZ expressed under the control of promoter P nisA ...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28169337/stoichiometry-and-structure-of-a-lantibiotic-maturation-complex
#2
Jens Reiners, André Abts, Rebecca Clemens, Sander H J Smits, Lutz Schmitt
Lantibiotics are ribosomally synthesized antimicrobial peptides secreted by mainly Gram-positive bacteria. Class 1 lantibiotics mature via two modification steps introduced by a modification LanBC complex. For the lantibiotic nisin, the dehydratase NisB catalyzes the dehydration of serine and threonine residues in the so-called core peptide. Second, five (methyl)-lanthionine rings are introduced in a regio- and stereospecific manner by the cyclase NisC. Here, we characterized the assembly of the NisBC complex in vitro, which is only formed in the presence of the substrate...
February 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28161626/turning-hydrophilic-bacteria-into-biorenewable-hydrophobic-material-with-potential-antimicrobial-activity-via-interaction-with-chitosan
#3
Orakan Hanpanich, Pravit Wongkongkatep, Thunyarat Pongtharangkul, Jirarut Wongkongkatep
Alteration of a bacteriocin-producing hydrophilic bacterium, Lactococcus lactis IO-1, into a hydrophobic material with potential antimicrobial activity using chitosan was investigated and compared with five other bacterial species with industrial importance. The negatively charged bacterial cells were neutralized by positively charged chitosan, resulting in a significant increase in the hydrophobicity of the bacterial cell surface. The largest Gram-positive B. megaterium ATCC 14581 showed a moderate response to chitosan while the smaller E...
January 27, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28135077/mechanistic-understanding-of-lanthipeptide-biosynthetic-enzymes
#4
Lindsay M Repka, Jonathan R Chekan, Satish K Nair, Wilfred A van der Donk
Lanthipeptides are ribosomally synthesized and post-translationally modified peptides (RiPPs) that display a wide variety of biological activities, from antimicrobial to antiallodynic. Lanthipeptides that display antimicrobial activity are called lantibiotics. The post-translational modification reactions of lanthipeptides include dehydration of Ser and Thr residues to dehydroalanine and dehydrobutyrine, a transformation that is carried out in three unique ways in different classes of lanthipeptides. In a cyclization process, Cys residues then attack the dehydrated residues to generate the lanthionine and methyllanthionine thioether cross-linked amino acids from which lanthipeptides derive their name...
January 30, 2017: Chemical Reviews
https://www.readbyqxmd.com/read/28032983/two-flavoenzymes-catalyze-the-post-translational-generation-of-5-chlorotryptophan-and-2-aminovinyl-cysteine-during-nai-107-biosynthesis
#5
Manuel A Ortega, Dillon P Cogan, Subha Mukherjee, Neha Garg, Bo Li, Gabrielle N Thibodeaux, Sonia I Maffioli, Stefano Donadio, Margherita Sosio, Jerome Escano, Leif Smith, Satish K Nair, Wilfred A van der Donk
Lantibiotics are ribosomally synthesized and post-translationally modified antimicrobial peptides containing thioether rings. In addition to these cross-links, the clinical candidate lantibiotic NAI-107 also possesses a C-terminal S-[(Z)-2-aminovinyl]-d-cysteine (AviCys) and a unique 5-chloro-l-tryptophan (ClTrp) moiety linked to its potent bioactivity. Bioinformatic and genetic analyses on the NAI-107 biosynthetic gene cluster identified mibH and mibD as genes encoding flavoenzymes responsible for the formation of ClTrp and AviCys, respectively...
February 17, 2017: ACS Chemical Biology
https://www.readbyqxmd.com/read/27914001/subtilosin-prevents-biofilm-formation-by-inhibiting-bacterial-quorum-sensing
#6
Ammar Algburi, Saskia Zehm, Victoria Netrebov, Anzhelica B Bren, Vladimir Chistyakov, Michael L Chikindas
Subtilosin, the cyclic lantibiotic protein produced by Bacillus subtilis KATMIRA1933, targets the surface receptor and electrostatically binds to the bacterial cell membrane. In this study, subtilosin was purified using ammonium sulfate ((NH4)2SO4) precipitation and purified via column chromatography. Subtilosin's antibacterial minimum and sub-minimum inhibitory concentrations (MIC and sub-MIC) and anti-biofilm activity (biofilm prevention) were established. Subtilosin was evaluated as a quorum sensing (QS) inhibitor in Gram-positive bacteria using Fe(III) reduction assay...
December 2, 2016: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/27902417/lantibiotics-produced-by-actinobacteria-and-their-potential-applications-a-review
#7
Karen Gomes, Rafael Silva Duarte, Maria do Carmo de Freire Bastos
The phylum Actinobacteria, which comprises a great variety of Gram-positive bacteria with a high G+C content in their genomes, is known for its large production of bioactive compounds, including those with antimicrobial activity. Among the antimicrobials, bacteriocins, ribosomally-synthesized peptides, represent an important arsenal of potential new drugs to face the increasing prevalence of resistance to antibiotics among microbial pathogens. The actinobacterial bacteriocins form a heterogeneous group of substances that is difficult to adapt to most proposed classification schemes...
November 22, 2016: Microbiology
https://www.readbyqxmd.com/read/27864176/insights-into-the-biosynthesis-of-duramycin
#8
Liujie Huo, Ayşe Ökesli, Ming Zhao, Wilfred A van der Donk
: Lantibiotics are ribosomally synthesized and posttranslationally modified antimicrobial peptides that are characterized by the thioether cross-linked bisamino acids lanthionine (Lan) and methyllanthionine (MeLan). Duramycin contains 19 amino acids, including one Lan and two MeLans, an unusual lysinoalanine (Lal) bridge formed from the ε-amino group of lysine 19 and a serine residue at position 6, and an erythro-3-hydroxy-l-aspartic acid at position 15. These modifications are important for the interactions of duramycin with its biological target, phosphatidylethanolamine (PE)...
February 1, 2017: Applied and Environmental Microbiology
https://www.readbyqxmd.com/read/27858169/a-novel-mechanism-of-immunity-controls-the-onset-of-cinnamycin-biosynthesis-in-streptomyces-cinnamoneus-dsm-40646
#9
Sean O'Rourke, David Widdick, Mervyn Bibb
Streptomyces cinnamoneus DSM 40646 produces the Class II lantibiotic cinnamycin which possesses an unusual mechanism of action, binding to the membrane lipid phosphatidylethanolamine (PE) to elicit its antimicrobial activity. A comprehensive analysis of the cinnamycin biosynthetic gene cluster has unveiled a novel mechanism of immunity in which the producing organism methylates its entire complement of PE prior to the onset of cinnamycin production. Deletion of the PE methyl transferase gene cinorf10, or the two-component regulatory system (cinKR) that controls its expression, leads not only to sensitivity to the closely related lantibiotic duramycin, but also abolishes cinnamycin production, presumably reflecting a fail-safe mechanism that serves to ensure that biosynthesis does not occur until immunity has been established...
November 17, 2016: Journal of Industrial Microbiology & Biotechnology
https://www.readbyqxmd.com/read/27856233/liars-reporter-assay-a-simple-tool-to-identify-lipid-ii-binding-moieties-in-lantibiotic-nukacin-isk-1
#10
Khaled M Elsayed, Mohammad R Islam, Abdullah-Al-Mahin, Jun-Ichi Nagao, Takeshi Zendo, Kenji Sonomoto
Binding to lipid II is an important step in the mode of action of most lantibiotics targeting the bacterial cell wall. We applied the Bacillus subtilis two-component system, LiaRS, that is known to respond to antibiotics interfering with lipid II cycle, in order to evaluate lipid II binding activity of known bacteriocins and also to identify lipid II binding moieties in lantibiotic nukacin ISK-1. Using this method, we confirmed that the methyllanthionine ring in nukacin ISK-1 is crucial for lipid II binding as previously indicated...
November 14, 2016: Journal of Bioscience and Bioengineering
https://www.readbyqxmd.com/read/27830473/mutagenesis-of-nisin-s-leader-peptide-proline-strongly-modulates-export-of-precursor-nisin
#11
Annechien Plat, Anneke Kuipers, Joe Crabb, Rick Rink, Gert N Moll
The lantibiotic nisin is produced by Lactococcus lactis as a precursor peptide comprising a 23 amino acid leader peptide and a 34 amino acid post-translationally modifiable core peptide. We previously demonstrated that the conserved FNLD part of the leader is essential for intracellular enzyme-catalyzed introduction of lanthionines in the core peptide and also for transporter-mediated export, whereas other positions are subject to large mutational freedom. We here demonstrate that, in the absence of the extracellular leader peptidase, NisP, export of precursor nisin via the modification and transporter enzymes, NisBTC, is strongly affected by multiple substitutions of the leader residue at position -2, but not by substitution of positions in the vicinity of this site...
November 10, 2016: Antonie Van Leeuwenhoek
https://www.readbyqxmd.com/read/27730566/antimicrobial-peptide-production-and-purification
#12
Srinivas Suda, Des Field, Niall Barron
Antimicrobial peptides (AMPs) are natural defense compounds which are synthesized as ribosomal gene-encoded pre-peptides and produced by all living organisms. AMPs are small peptides, usually cationic and typically have hydrophobic residues which interact with cell membranes and have either a narrow or broad spectrum of biological activity. AMPs are isolated from the natural host or heterologously expressed in other hosts such as Escherichia coli. The proto-typical lantibiotic Nisin is a widely used AMP that is produced by the food-grade organism Lactococcus lactis...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27697045/bacterial-labionin-containing-peptides-and-sactibiotics-unusual-types-of-antimicrobial-peptides-with-potential-use-in-clinical-settings-a-review
#13
Marcus Lívio Varella Coelho, Andreza Freitas de Souza Duarte, Maria do Carmo de Freire Bastos
One of the biggest challenges faced presently by clinicians is the emergence of multi drug--resistant pathogens that can infect humans and animals.To control the infections caused by such pathogens the development of new drugs is required. Bacteria are a rich source of ribosomally-synthesized antimicrobial peptides known as bacteriocins, which are characterized by the presence of a self-defense immunity system. Labionin-containing lantibiotics and sactibiotics are post-translationally modified bacteriocins with peculiar features...
September 30, 2016: Current Topics in Medicinal Chemistry
https://www.readbyqxmd.com/read/27693589/probing-phosphoethanolamine-containing-lipids-in-membranes-with-duramycin-cinnamycin-and-aegerolysin-proteins
#14
REVIEW
Françoise Hullin-Matsuda, Asami Makino, Motohide Murate, Toshihide Kobayashi
In this mini-review, we summarize current knowledge about the lipid-binding characteristics of two types of toxins used to visualize the membrane distribution of phosphoethanolamine-containing lipid species: the glycerophospholipid, phosphatidylethanolamine (PE) and the sphingolipid, ceramide phosphoethanolamine (CPE). The lantibiotic cinnamycin and the structurally-related peptide duramycin produced by some Gram-positive bacteria were among the first toxins characterized by their specificity for PE which is widely present in animal kingdoms from bacteria to mammals...
November 2016: Biochimie
https://www.readbyqxmd.com/read/27684443/deferred-growth-inhibition-assay-to-quantify-the-effect-of-bacteria-derived-antimicrobials-on-competition
#15
Josephine C Moran, Emma L Crank, Hanaa A Ghabban, Malcolm J Horsburgh
Competitive exclusion can occur in microbial communities when, for example, an inhibitor-producing strain outcompetes its competitor for an essential nutrient or produces antimicrobial compounds that its competitor is not resistant to. Here we describe a deferred growth inhibition assay, a method for assessing the ability of one bacterium to inhibit the growth of another through the production of antimicrobial compounds or through competition for nutrients. This technique has been used to investigate the correlation of nasal isolates with the exclusion of particular species from a community...
September 3, 2016: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27664721/bacillus-velezensis-rc-218-as-a-biocontrol-agent-to-reduce-fusarium-head-blight-and-deoxynivalenol-accumulation-genome-sequencing-and-secondary-metabolite-cluster-profiles
#16
Juan M Palazzini, Christopher A Dunlap, Michael J Bowman, Sofía N Chulze
Bacillus subtilis RC 218 was originally isolated from wheat anthers as a potential antagonist of Fusarium graminearum, the causal agent of Fusarium head blight (FHB). It was demonstrated to have antagonist activity against the plant pathogen under in vitro and greenhouse assays. The current study extends characterizing B. subtilis RC 218 with a field study and genome sequencing. The field study demonstrated that B. subtilis RC 218 could reduce disease severity and the associated mycotoxin (deoxynivalenol) accumulation, under field conditions...
November 2016: Microbiological Research
https://www.readbyqxmd.com/read/27634138/towards-biocontained-cell-factories-an-evolutionarily-adapted-escherichia-coli-strain-produces-a-new-to-nature-bioactive-lantibiotic-containing-thienopyrrole-alanine
#17
Anja Kuthning, Patrick Durkin, Stefan Oehm, Michael G Hoesl, Nediljko Budisa, Roderich D Süssmuth
Genetic code engineering that enables reassignment of genetic codons to non-canonical amino acids (ncAAs) is a powerful strategy for enhancing ribosomally synthesized peptides and proteins with functions not commonly found in Nature. Here we report the expression of a ribosomally synthesized and post-translationally modified peptide (RiPP), the 32-mer lantibiotic lichenicidin with a canonical tryptophan (Trp) residue replaced by the ncAA L-β-(thieno[3,2-b]pyrrolyl)alanine ([3,2]Tpa) which does not sustain cell growth in the culture...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27591436/employing-the-promiscuity-of-lantibiotic-biosynthetic-machineries-to-produce-novel-antimicrobials
#18
REVIEW
Manuel Montalbán-López, Auke J van Heel, Oscar P Kuipers
As the number of new antibiotics that reach the market is decreasing and the demand for them is rising, alternative sources of novel antimicrobials are needed. Lantibiotics are potent peptide antimicrobials that are ribosomally synthesized and stabilized by post-translationally introduced lanthionine rings. Their ribosomal synthesis and enzymatic modifications provide excellent opportunities to design and engineer a large variety of novel antimicrobial compounds. The research conducted in this area demonstrates that the modularity present in both the peptidic rings as well as in the combination of promiscuous modification enzymes can be exploited to further increase the diversity of lantibiotics...
September 2, 2016: FEMS Microbiology Reviews
https://www.readbyqxmd.com/read/27526944/new-insights-into-the-mode-of-action-of-the-lantibiotic-salivaricin-b
#19
Abdelahhad Barbour, John Tagg, Osama K Abou-Zied, Koshy Philip
Salivaricin B is a 25 amino acid polycyclic peptide belonging to the type AII lantibiotics and first shown to be produced by Streptococcus salivarius. In this study we describe the bactericidal mode of action of salivaricin B against susceptible Gram-positive bacteria. The killing action of salivaricin B required micro-molar concentrations of lantibiotic whereas the prototype lantibiotic nisin A was shown to be potent at nano-molar levels. Unlike nisin A, salivaricin B did not induce pore formation or dissipate the membrane potential in susceptible cells...
2016: Scientific Reports
https://www.readbyqxmd.com/read/27524178/evolutionary-architecture-of-the-infant-adapted-group-of-bifidobacterium-species-associated-with-the-probiotic-function
#20
Min-Jung Kwak, Soon-Kyeong Kwon, Jae-Kyung Yoon, Ju Yeon Song, Jae-Gu Seo, Myung Jun Chung, Jihyun F Kim
Bifidobacteria, often associated with the gastrointestinal tract of animals, are well known for their roles as probiotics. Among the dozens of Bifidobacterium species, Bifidobacterium bifidum, B. breve, and B. longum are the ones most frequently isolated from the feces of infants and known to help the digestion of human milk oligosaccharides. To investigate the correlation between the metabolic properties of bifidobacteria and their phylogeny, we performed a phylogenomic analysis based on 452 core genes of forty-four completely sequenced Bifidobacterium species...
October 2016: Systematic and Applied Microbiology
keyword
keyword
78664
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"