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https://www.readbyqxmd.com/read/27812090/diagnosis-of-persistent-fever-in-the-tropics-set-of-standard-operating-procedures-used-in-the-nidiag-febrile-syndrome-study
#1
Emilie Alirol, Ninon Seiko Horie, Barbara Barbé, Veerle Lejon, Kristien Verdonck, Philippe Gillet, Jan Jacobs, Philippe Büscher, Basudha Kanal, Narayan Raj Bhattarai, Sayda El Safi, Thong Phe, Kruy Lim, Long Leng, Pascal Lutumba, Deby Mukendi, Emmanuel Bottieau, Marleen Boelaert, Suman Rijal, François Chappuis
In resource-limited settings, the scarcity of skilled personnel and adequate laboratory facilities makes the differential diagnosis of fevers complex [1-5]. Febrile illnesses are diagnosed clinically in most rural centers, and both Rapid Diagnostic Tests (RDTs) and clinical algorithms can be valuable aids to health workers and facilitate therapeutic decisions [6,7]. The persistent fever syndrome targeted by NIDIAG is defined as presence of fever for at least one week. The NIDIAG clinical research consortium focused on potentially severe and treatable infections and therefore targeted the following conditions as differential diagnosis of persistent fever: visceral leishmaniasis (VL), human African trypanosomiasis (HAT), enteric (typhoid and paratyphoid) fever, brucellosis, melioidosis, leptospirosis, malaria, tuberculosis, amoebic liver abscess, relapsing fever, HIV/AIDS, rickettsiosis, and other infectious diseases (e...
November 2016: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/27717815/reorganization-of-motor-cortex-and-impairment-of-motor-performance-induced-by-hindlimb-unloading-are-partially-reversed-by-cortical-igf-1-administration
#2
Julien Mysoet, Marie-Hélène Canu, Christophe Gillet, Julie Fourneau, Cyril Garnier, Bruno Bastide, Erwan Dupont
Immobilization, bed rest, or sedentary lifestyle, are known to induce a profound impairment in sensorimotor performance. These alterations are due to a combination of peripheral and central factors. Previous data conducted on a rat model of disuse (hindlimb unloading, HU) have shown a profound reorganization of motor cortex and an impairment of motor performance. Recently, our interest was turned towards the role of insulin-like growth factor 1 (IGF-1) in cerebral plasticity since this growth factor is considered as the mediator of beneficial effects of exercise on the central nervous system, and its cortical level is decreased after a 14-day period of HU...
October 4, 2016: Behavioural Brain Research
https://www.readbyqxmd.com/read/27701404/a-multicenter-study-benchmarks-software-tools-for-label-free-proteome-quantification
#3
Pedro Navarro, Jörg Kuharev, Ludovic C Gillet, Oliver M Bernhardt, Brendan MacLean, Hannes L Röst, Stephen A Tate, Chih-Chiang Tsou, Lukas Reiter, Ute Distler, George Rosenberger, Yasset Perez-Riverol, Alexey I Nesvizhskii, Ruedi Aebersold, Stefan Tenzer
Consistent and accurate quantification of proteins by mass spectrometry (MS)-based proteomics depends on the performance of instruments, acquisition methods and data analysis software. In collaboration with the software developers, we evaluated OpenSWATH, SWATH 2.0, Skyline, Spectronaut and DIA-Umpire, five of the most widely used software methods for processing data from sequential window acquisition of all theoretical fragment-ion spectra (SWATH)-MS, which uses data-independent acquisition (DIA) for label-free protein quantification...
November 2016: Nature Biotechnology
https://www.readbyqxmd.com/read/27699207/slow-cholinergic-modulation-of-spike-probability-in-ultra-fast-time-coding-sensory-neurons
#4
David Goyer, Stefanie Kurth, Charlène Gillet, Christian Keine, Rudolf Rübsamen, Thomas Kuenzel
Sensory processing in the lower auditory pathway is generally considered to be rigid and thus less subject to modulation than central processing. However, in addition to the powerful bottom-up excitation by auditory nerve fibers, the ventral cochlear nucleus also receives efferent cholinergic innervation from both auditory and nonauditory top-down sources. We thus tested the influence of cholinergic modulation on highly precise time-coding neurons in the cochlear nucleus of the Mongolian gerbil. By combining electrophysiological recordings with pharmacological application in vitro and in vivo, we found 55-72% of spherical bushy cells (SBCs) to be depolarized by carbachol on two time scales, ranging from hundreds of milliseconds to minutes...
September 2016: ENeuro
https://www.readbyqxmd.com/read/27634586/long-term-stability-of-dexamethasone-and-alizapride-or-ondansetron-in-sodium-chloride-0-9-polyolefin-bag-at-5%C3%A2-3%C3%A2-c
#5
J Simar, M Godet, J-D Hecq, M Closset, P Gillet, C Langhendries, B Bihin, J Jamart, L Galanti
INTRODUCTION: The aim of the study was to investigate the long-term stability of dexamethasone 10mg associated with alizapride 100mg or ondansetron 8mg in 100mL of 0.9% sodium chloride solution stored at 5±3°C. METHOD: Solutions of 0.9% sodium chloride 100mL in polyolefin bags (n=5) containing approximately dexamethasone (DEX) 10mg associated with alizapride (ALI) 100mg or ondansetron (OND) 8mg were prepared under aseptic conditions and stored about 30 days at 5±3°C...
September 12, 2016: Annales Pharmaceutiques Françaises
https://www.readbyqxmd.com/read/27611912/electronic-coupling-calculations-for-bridge-mediated-charge-transfer-using-constrained-density-functional-theory-cdft-and-effective-hamiltonian-approaches-at-the-density-functional-theory-dft-and-fragment-orbital-density-functional-tight-binding-fodftb-level
#6
Natacha Gillet, Laura Berstis, Xiaojing Wu, Fruzsina Gajdos, Alexander Heck, Aurélien de la Lande, Jochen Blumberger, Marcus Elstner
In this article, four methods to calculate charge transfer integrals in the context of bridge-mediated electron transfer are tested. These methods are based on density functional theory (DFT). We consider two perturbative Green's function effective Hamiltonian methods (first, at the DFT level of theory, using localized molecular orbitals; second, applying a tight-binding DFT approach, using fragment orbitals) and two constrained DFT implementations with either plane-wave or local basis sets. To assess the performance of the methods for through-bond (TB)-dominated or through-space (TS)-dominated transfer, different sets of molecules are considered...
October 11, 2016: Journal of Chemical Theory and Computation
https://www.readbyqxmd.com/read/27572612/new-parameters-on-the-hematology-analyzer-xn-10-sysmextm-allow-to-distinguish-childhood-bacterial-and-viral-infections
#7
I Henriot, E Launay, M Boubaya, L Cremet, M Illiaquer, H Caillon, A Desjonquères, B Gillet, M C Béné, M Eveillard
INTRODUCTION: Complete blood counts (CBC) performed for infected children admitted for fever mostly disclose leukocytosis. Yet, the recently developed XN-10(®) provides novel CBC parameters which could be useful to ascertain infection and discriminate between bacterial and viral etiologies. These were the main objectives of the study presented here. METHODS: Blood samples from 90 children, 1 month to 5 years old, admitted to an emergency unit for fever benefited from a CBC, C-reactive protein, and procalcitonin assays...
August 30, 2016: International Journal of Laboratory Hematology
https://www.readbyqxmd.com/read/27107463/long-term-stability-of-ketamine-hydrochloride-50mg-ml-injection-in-3ml-syringes
#8
S Huvelle, M Godet, J-D Hecq, P Gillet, B Bihin, J Jamart, L Galanti
INTRODUCTION: Ketamine hydrochloride (Ketalar(®)) injection is often used as a general anesthetic agent. It is particularly suited to short-term interventions. It can also be used as an inducer of anesthesia before the administration of other anesthetic agents. The aim of this study was to evaluate the stability of ketamine hydrochloride in 3ml polypropylene syringes after storage for up to 180days at room temperature. METHOD: Syringes containing ketamine hydrochloride (50mg/ml) were prepared and stored at room temperature (25°C) for 180days...
July 2016: Annales Pharmaceutiques Françaises
https://www.readbyqxmd.com/read/27105311/multiple-electrode-aggregometry-is-an-adequate-method-for-aspirin-response-testing-in-myeloproliferative-neoplasms-and-differentiates-the-mechanisms-of-aspirin-resistance
#9
Benjamin Gillet, Jean-Christophe Ianotto, Fanny Mingant, Romain Didier, Martine Gilard, Valérie Ugo, Eric Lippert, Hubert Galinat
INTRODUCTION: In myeloproliferative neoplasms (MPN), aspirin reduces the thrombotic risk. Nevertheless, aspirin resistance may be due to excessive platelet production ("turnover" resistance). Aspirin resistance can also result from a lack of effect of aspirin; this second component is called "intrinsic resistance". Two biological tests are considered reference methods for the assessment of aspirin resistance: TXB2 assay and Light Transmittance Aggregometry (LTA) with arachidonic acid...
June 2016: Thrombosis Research
https://www.readbyqxmd.com/read/27069582/recent-changes-in-mountain-grasslands-a-vegetation-resampling-study
#10
François Gillet, Leslie Mauchamp, Pierre-Marie Badot, Arnaud Mouly
Understanding how land-use changes affect different facets of plant biodiversity in seminatural European grasslands is of particular importance for biodiversity conservation. As conclusions of previous experimental or synchronic observational studies did not converge toward a general agreement, assessing the recent trends in vegetation change in various grassland systems using a diachronic approach is needed. In this resurvey study, we investigated the recent changes in grassland vegetation of the French Jura Mountains, a region with a long tradition of pastoralism...
April 2016: Ecology and Evolution
https://www.readbyqxmd.com/read/27049628/mass-spectrometry-applied-to-bottom-up-proteomics-entering-the-high-throughput-era-for-hypothesis-testing
#11
Ludovic C Gillet, Alexander Leitner, Ruedi Aebersold
Proteins constitute a key class of molecular components that perform essential biochemical reactions in living cells. Whether the aim is to extensively characterize a given protein or to perform high-throughput qualitative and quantitative analysis of the proteome content of a sample, liquid chromatography coupled to tandem mass spectrometry has become the technology of choice. In this review, we summarize the current state of mass spectrometry applied to bottom-up proteomics, the approach that focuses on analyzing peptides obtained from proteolytic digestion of proteins...
June 12, 2016: Annual Review of Analytical Chemistry
https://www.readbyqxmd.com/read/27015830/a-new-method-using-insert-based-systems-ibs-to-improve-cell-behavior-study-on-flexible-and-rigid-biomaterials
#12
Charlotte Grenade, Nicolas Moniotte, Eric Rompen, Alain Vanheusden, Amélie Mainjot, Marie-Claire De Pauw-Gillet
In vitro studies about biomaterials biological properties are essential screening tests. Yet cell cultures encounter difficulties related to cell retention on material surface or to the observation of both faces of permeable materials. The objective of the present study was to develop a reliable in vitro method to study cell behavior on rigid and flexible/permeable biomaterials elaborating two specific insert-based systems (IBS-R and IBS-F respectively). IBS-R was designed as a specific cylindrical polytetrafluoroethylene (PTFE) system to evaluate attachment, proliferation and morphology of human gingival fibroblasts (HGFs) on grade V titanium and lithium disilicate glass-ceramic discs characteristics of dental prostheses...
December 2016: Cytotechnology
https://www.readbyqxmd.com/read/26994554/a-new-method-for-the-separation-and-purification-of-the-osteogenic-compounds-of-nacre-ethanol-soluble-matrix
#13
G Zhang, A S Willemin, A Brion, M H Piet, V Moby, A Bianchi, D Mainard, L Galois, P Gillet, M Rousseau
Nacre is able to induce bone-forming cells mineralization, and gains widely interest in bone regeneration. While, the osteoinductive compounds are not yet identified. ESM (Ethanol Soluble Matrix), a nacre extract from powder of Pinctada margaritifera pearl oyster shell, has been firstly proven having the capacity to induce mineralization and to restore mineralization defect in vitro. It is suitable to treat ESM as a source of osteoinductive compounds. Herein, we develop a new method for separating and purifying nacre extracts by an ionic approach...
March 16, 2016: Journal of Structural Biology
https://www.readbyqxmd.com/read/26947910/-which-place-for-point-of-care-screening-tests-in-the-diagnosis-of-hepatitis-c-infection-among-drug-users
#14
Noémie Lacroix, Hélène Barraud, Claudine Gillet, Paolo Di Patrizio, Jean Pierre Bronowicki, Raymund Schwan, Vincent Laprévote
Hepatitis C is a severe disease, which often evolves into chronicity and for which there is no vaccine available. Therefore its screening is essential, especially among drug users who are the main reservoir of the hepatitis C virus (HCV). Current guidelines for screening are based on the detection of total anti-HCV antibodies (Ab) by means of third generation EIA. This test is performed in a laboratory from a venous sample. Alternative methods have been recently developed, including point-of-care tests (POCT) that offer many advantages...
April 2016: La Presse Médicale
https://www.readbyqxmd.com/read/26714368/long-term-stability-of-cocaine-hydrochloride-aqueous-solution-50-mg-ml-5-at-room-temperature-and-at-5%C3%A2-c-%C3%A2-3%C3%A2-c-in-glass-bottles
#15
Joseph Dewulf, Jean-Daniel Hecq, Sophie Huvelle, Marie Godet, Patricia Gillet, Jacques Jamart, Laurence M Galanti
The objective of this study was to investigate the stability of cocaine hydrochloride 50 mg/mL (5%) aqueous solution stored in glass bottles at 5°C ± 3°C or at room temperature. Ten bottles of cocaine hydrochloride 50 mg/mL were prepared under aseptic conditions. Five were stored at 5°C ± 3°C and five at room temperature. pH measurements, optic density measurements at different wave-lengths, and optic microscopic observations were performed periodically during the storage. A forced-degradation test with hydrochloric acid 12M and NaOH 5M with and without heating at 100°C was also performed...
May 2015: International Journal of Pharmaceutical Compounding
https://www.readbyqxmd.com/read/26693214/assessment-of-consistency-between-the-arm-fossa-test-and-gillet-test-a-pilot-study
#16
Robert Cooperstein, Charles Blum, Elaine C Cooperstein
OBJECTIVE: The purpose of this pilot study was to test methods needed to conduct a study with adequate power to investigate consistency between the arm-fossa test (AFT) and the Gillet test. METHODS: A convenience sample of chiropractic college students enrolled in a weekend Sacro-Occipital Technique seminar participated. Each was tested with AFT and sacroiliac orthopedic tests, including the Gillet test. Statistical testing included calculation of κ for consistency of the AFT and Gillet test and their diagnostic efficiency...
March 2015: Journal of Chiropractic Medicine
https://www.readbyqxmd.com/read/26144898/a-versatile-in-vitro-elisa-test-for-quantification-and-quality-testing-of-infectious-inactivated-and-formulated-rabies-virus-used-in-veterinary-monovalent-or-combination-vaccine
#17
Cécile Sigoillot-Claude, Myriam Battaglio, Marc Fiorucci, Delphine Gillet, Anne-Sophie Vimort, Yves Giraud, Sonia Laurent, Alain Vaganay, Hervé Poulet
Regulatory potency test for rabies vaccines requires mice vaccination followed by challenge with a live virus via intracerebral route. An alternative in vitro test, consistent with the "3R's" (Reduce, Replace, Refine) was designed to quantify active glycoprotein G using seroneutralizing monoclonal antibodies. This versatile ELISA targets well conformed neutralizing epitopes. Therefore, it quantifies only the trimeric pre-fusion form of glycoprotein G known to elicits the production of viral neutralizing antibodies...
July 31, 2015: Vaccine
https://www.readbyqxmd.com/read/26143547/enhanced-precision-of-ankle-torque-measure-with-an-open-unit-dynamometer-mounted-with-a-3d-force-torque-sensor
#18
A Toumi, S Leteneur, C Gillet, J-F Debril, N Decoufour, F Barbier, J M Jakobi, Emilie Simoneau-Buessinger
PURPOSE: Many studies have focused on maximum torque exerted by ankle joint muscles during plantar flexion. While strength parameters are typically measured with isokinetic or isolated ankle dynamometers, these devices often present substantial limitations for the measurement of torque because they account for force in only 1 dimension (1D), and the device often constrains the body in a position that augments torque through counter movements. The purposes of this study were to determine the contribution of body position to ankle plantar-flexion torque and to assess the use of 1D and 3D torque sensors...
November 2015: European Journal of Applied Physiology
https://www.readbyqxmd.com/read/26116376/progress-and-challenges-in-simulating-and-understanding-electron-transfer-in-proteins
#19
REVIEW
Aurélien de la Lande, Natacha Gillet, Shufeng Chen, Dennis R Salahub
This Review presents an overview of the most common numerical simulation approaches for the investigation of electron transfer (ET) in proteins. We try to highlight the merits of the different approaches but also the current limitations and challenges. The article is organized into three sections. Section 2 deals with direct simulation algorithms of charge migration in proteins. Section 3 summarizes the methods for testing the applicability of the Marcus theory for ET in proteins and for evaluating key thermodynamic quantities entering the reaction rates (reorganization energies and driving force)...
September 15, 2015: Archives of Biochemistry and Biophysics
https://www.readbyqxmd.com/read/26040677/tif1%C3%AE-interferes-with-tgf%C3%AE-1-smad4-signaling-to-promote-poor-outcome-in-operable-breast-cancer-patients
#20
Loay Kassem, Mathieu Deygas, Laurent Fattet, Jonathan Lopez, Thibaut Goulvent, Emilie Lavergne, Sylvie Chabaud, Nicolas Carrabin, Nicolas Chopin, Thomas Bachelot, Germain Gillet, Isabelle Treilleux, Ruth Rimokh
BACKGROUND: The Transforming growth factor β (TGFβ) signaling has a paradoxical role in cancer development and outcome. Besides, the prognostic significance of the TGFβ1, SMAD4 in breast cancer patients is an area of many contradictions. The transcriptional intermediary factor 1γ (TIF1γ) is thought to interact with the TGFβ/SMAD signaling through different mechanisms. Our study aims to define the prognostic significance of TGFβ1, SMAD4 and TIF1γ expression in breast cancer patients and to detect possible interactions among those markers that might affect the outcome...
2015: BMC Cancer
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