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https://www.readbyqxmd.com/read/29054504/proteomics-in-plasma-of-ovariectomized-rats-and-those-exposed-to-estradiol-valerate
#1
Hai Jiang, Wenpei Bai, Wenjuan Wang, Ke Wang, Jing Jia, Jing Zhang, He Diao, Lihua Qin
The menopausal period, an inevitable physiological process for women, is frequently associated with physiological and psychological dysfunction attributable to substantial fluctuation and gradual decrease in female hormones induced by ovarian failure, leading to corresponding symptoms and diseases that impact multiple systems in the body to varying degrees. As prior studies have focused primarily on menopausal syndrome-related pathophysiological changes and hormone replacement therapy effects, here we approached menopausal disease incidence risk and pathogenesis through systemic plasma proteomics analysis...
October 17, 2017: Journal of Steroid Biochemistry and Molecular Biology
https://www.readbyqxmd.com/read/29053841/meat-quality-traits-and-proteome-profile-of-woody-broiler-breast-pectoralis-major-meat
#2
K Cai, W Shao, X Chen, Y L Campbell, M N Nair, S P Suman, C M Beach, M C Guyton, M W Schilling
Woody breast meat has recently become prevalent in the broiler industry in both the United States and European Union. Recent publications have described the meat quality characteristics of woody breast meat as having hardened areas and pale ridge-like bulges at both the caudal and cranial regions of the breast. The present study investigated the meat quality (pH, color, cooking loss, and shear force) and protein quality characteristics (protein and salt-soluble protein content) in woody breast meat as compared to normal breast meat...
October 5, 2017: Poultry Science
https://www.readbyqxmd.com/read/29053587/heat-responsive-photosynthetic-and-signaling-pathways-in-plants-insight-from-proteomics
#3
REVIEW
Xiaoli Wang, Chenxi Xu, Xiaofeng Cai, Quanhua Wang, Shaojun Dai
Heat stress is a major abiotic stress posing a serious threat to plants. Heat-responsive mechanisms in plants are complicated and fine-tuned. Heat signaling transduction and photosynthesis are highly sensitive. Therefore, a thorough understanding of the molecular mechanism in heat stressed-signaling transduction and photosynthesis is necessary to protect crop yield. Current high-throughput proteomics investigations provide more useful information for underlying heat-responsive signaling pathways and photosynthesis modulation in plants...
October 20, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29051082/magnetic-capture-of-polydopamine-encapsulated-hela-cells-for-the-analysis-of-cell-surface-proteins
#4
Yiying Liu, Guoquan Yan, Mingxia Gao, Xiangmin Zhang
A novel method to characterize cell surface proteins and complexes has been developed. Polydopamine (PDA)-encapsulated Hela cells were prepared for plasma membrane proteome research. Since the PDA protection, the encapsulated cells could be maintained for more than two weeks. Amino groups functionalized magnetic nanoparticles were also used for cell capture by the reaction with the PDA coatings. Plasma membrane fragments were isolated and enriched with assistance of an external magnetic field after disruption of the coated cells by ultrasonic treatment...
October 16, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29050943/mir-67-regulates-p-aeruginosa-avoidance-behavior-in-c-elegans
#5
Yi-Cheng Ma, Lu Zhang, Li-Li Dai, Rahat Ullah Khan, Cheng-Gang Zou
Pathogen avoidance behaviors are found throughout the animal kingdom and are important for animal's survival in nature. As a free-living nematode, C. elegans is exposed to a variety of microorganisms, including toxic or pathogenic bacteria, in soil. C. elegans can develop efficient avoidance responses to pathogenic bacteria to minimize the infection risk. However, the role of microRNAs (miRNAs) in pathogen avoidance in C. elegans remains unclear. In this report, we showed that the miRNA mir-67 was involved in a behavioral avoidance response to P...
October 16, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29050296/reverse-phase-protein-array-identification-of-triple-negative-breast-cancer-subtypes-and-comparison-with-mrna-molecular-subtypes
#6
Hiroko Masuda, Yuan Qi, Shuying Liu, Naoki Hayashi, Takahiro Kogawa, Gabriel N Hortobagyi, Debu Tripathy, Naoto T Ueno
BACKGROUND: Reverse phase protein array (RPPA) analysis, allows investigation of potential targets at the functional protein level,. We identified TNBC subtypes at the protein level using RPPA and compared them with mRNA molecular subtypes (TNBCtype, TNBCtype-4, and PAM50) that is unique in its availability of both RPPA and mRNA analyses. METHODS: We classified the samples from 80 TNBC patients using both k-means and hierarchical consensus clustering analysis and performed Ingenuity Pathway Analysis...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29050215/proteomics-analysis-of-bladder-cancer-invasion-targeting-eif3d-for-therapeutic-intervention
#7
Agnieszka Latosinska, Marika Mokou, Manousos Makridakis, William Mullen, Jerome Zoidakis, Vasiliki Lygirou, Maria Frantzi, Ioannis Katafigiotis, Konstantinos Stravodimos, Marie C Hupe, Maciej Dobrzynski, Walter Kolch, Axel S Merseburger, Harald Mischak, Maria G Roubelakis, Antonia Vlahou
Patients with advanced bladder cancer have poor outcomes, indicating a need for more efficient therapeutic approaches. This study characterizes proteomic changes underlying bladder cancer invasion aiming for the better understanding of disease pathophysiology and identification of drug targets. High resolution liquid chromatography coupled to tandem mass spectrometry analysis of tissue specimens from patients with non-muscle invasive (NMIBC, stage pTa) and muscle invasive bladder cancer (MIBC, stages pT2+) was conducted...
September 19, 2017: Oncotarget
https://www.readbyqxmd.com/read/29046415/skeletal-stiffening-in-an-amphibious-fish-out-of-water-is-a-response-to-increased-body-weight
#8
Andy J Turko, Dietmar Kültz, Douglas Fudge, Roger P Croll, Frank M Smith, Matthew R Stoyek, Patricia A Wright
Terrestrial animals must support their bodies against gravity, while aquatic animals are effectively weightless because of buoyant support from water. Given this evolutionary history of minimal gravitational loading of fishes in water, it has been hypothesized that weight-responsive musculoskeletal systems evolved during the tetrapod invasion of land and are thus absent in fishes. Amphibious fishes, however, experience increased effective weight when out of water - are these fishes responsive to gravitational loading? Contrary to the tetrapod-origin hypothesis, we found that terrestrial acclimation reversibly increased gill arch stiffness (∼60% increase) in the amphibious fish Kryptolebias marmoratus when loaded normally by gravity, but not under simulated microgravity...
October 15, 2017: Journal of Experimental Biology
https://www.readbyqxmd.com/read/29046343/complement-activation-in-peritoneal-dialysis-induced-arteriolopathy
#9
Maria Bartosova, Betti Schaefer, Justo Lorenzo Bermejo, Silvia Tarantino, Felix Lasitschka, Stephan Macher-Goeppinger, Peter Sinn, Bradley A Warady, Ariane Zaloszyc, Katja Parapatics, Peter Májek, Keiryn L Bennett, Jun Oh, Christoph Aufricht, Franz Schaefer, Klaus Kratochwill, Claus Peter Schmitt
Cardiovascular disease (CVD) is the leading cause of increased mortality in patients with CKD and is further aggravated by peritoneal dialysis (PD). Children are devoid of preexisting CVD and provide unique insight into specific uremia- and PD-induced pathomechanisms of CVD. We obtained peritoneal specimens from children with stage 5 CKD at time of PD catheter insertion (CKD5 group), children with established PD (PD group), and age-matched nonuremic controls (n=6/group). We microdissected omental arterioles from tissue layers not directly exposed to PD fluid and used adjacent sections of four arterioles per patient for transcriptomic and proteomic analyses...
October 18, 2017: Journal of the American Society of Nephrology: JASN
https://www.readbyqxmd.com/read/29043805/the-mitogen-activated-protein-kinase-kinase-5-regulates-the-proliferation-and-biosynthetic-processes-in-procyclic-forms-of-trypanosoma-brucei
#10
Fernanda G Kugeratski, Michel Batista, Carla Vanessa de Paula Lima, Lisa J Neilson, Elizabeth Sousa da Cunha, Lyris M F de Godoy, Sara Zanivan, Marco Aurelio Krieger, Fabricio K Marchini
The pathogenic protozoan T. brucei alternates into distinct developmental stages in the mammalian and insect hosts. The mitogen-activated protein kinase (MAPK) signaling pathways transduce extracellular stimuli into a range of cellular responses, which ultimately lead to the adaptation to the external environment. Here, we combined a loss of function approach with stable isotope labelling with amino acids in cell culture (SILAC)-based mass spectrometry (MS) to investigate the role of the mitogen-activated kinase kinase 5 (MKK5) in T...
October 18, 2017: Journal of Proteome Research
https://www.readbyqxmd.com/read/29042637/the-polyglutamine-protein-ataxin-3-enables-normal-growth-under-heat-shock-conditions-in-the-methylotrophic-yeast-pichia-pastoris
#11
Marcella Bonanomi, Valentina Roffia, Antonella De Palma, Alessio Lombardi, Francesco Antonio Aprile, Cristina Visentin, Paolo Tortora, Pierluigi Mauri, Maria Elena Regonesi
The protein ataxin-3 carries a polyglutamine stretch close to the C-terminus that triggers a neurodegenerative disease in humans when its length exceeds a critical threshold. A role as a transcriptional regulator but also as a ubiquitin hydrolase has been proposed for this protein. Here, we report that, when expressed in the yeast Pichia pastoris, full-length ataxin-3 enabled almost normal growth at 37 °C, well above the physiological optimum of 30 °C. The N-terminal Josephin domain (JD) was also effective but significantly less, whereas catalytically inactive JD was completely ineffective...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29042595/a-plasma-proteomics-method-reveals-links-between-ischemic-stroke-and-mthfr-c677t-genotype
#12
Zhenchang Zhang, Qi Yan, Jia Guo, Xueping Wang, Wei Yuan, Lei Wang, Lixia Chen, Gang Su, Manxia Wang
Methylene Tetrahydrofolate Reductase (MTHFR) catalyzes the conversion of methylene tetrahydrofolate to methylte trahydrofolate. The 677th nucleotide of the MTHFR gene is often regarded as a risk factor of cardiovascular disease. Previous studies demonstrated an elevated risk of ischemic stroke with the MTHFR677TT genotype. In this study, we employed a plasma proteomics method to investigate the connection between the polymorphism of the target nucleotide and stroke. In total, 28 protein spots were differentially expressed between the two groups, and of which, 25 protein spots were up-regulated and 3 were down-regulated...
October 17, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29041828/urine-protein-biomarkers-for-detection-of-cardiovascular-disease-and-their-use-for-the-clinic
#13
Sarah Röthlisberger, Johanna Pedroza-Diaz
Cardiovascular disease (CVD) is the leading noncommunicable disease and main cause of death worldwide. Traditionally, blood has been the sample of choice for biomarker discovery, however, urine has roused great interest in recent years as a source of biomarkers. Sample collection is simple, non-invasive, and there is the possibility of implementing minimal cost tests in primary care settings. Areas covered: In this review, we systematically searched PubMed for proteomic studies of CVD, with the criteria that urine was included as a biological sample...
October 18, 2017: Expert Review of Proteomics
https://www.readbyqxmd.com/read/29040838/nutrient-sensing-and-metabolic-changes-after-methionine-deprivation-in-primary-muscle-cells-of-turbot-scophthalmus-maximus-l
#14
Haowen Jiang, Fuyun Bian, Huihui Zhou, Xuan Wang, Kaidi Wang, Kangsen Mai, Gen He
The low methionine content in plant-based diets is a major limiting factor for feed utilization by animals. However, the molecular consequences triggered by methionine deficiency have not been well characterized, especially in fish species, whose metabolism is unique in many aspects and important for aquaculture industry. In the present study, the primary muscle cells of turbot (Scophthalmus maximus L.) were isolated and treated with or without methionine for 12 h in culture. The responses of nutrient sensing pathways, the proteomic profiling of metabolic processes, and the expressions of key metabolic molecules were systematically examined...
September 14, 2017: Journal of Nutritional Biochemistry
https://www.readbyqxmd.com/read/29040688/the-online-tabloid-proteome-an-annotated-database-of-protein-associations
#15
Surya Gupta, Demet Turan, Jan Tavernier, Lennart Martens
A complete knowledge of the proteome can only be attained by determining the associations between proteins, along with the nature of these associations (e.g. physical contact in protein-protein interactions, participation in complex formation or different roles in the same pathway). Despite extensive efforts in elucidating direct protein interactions, our knowledge on the complete spectrum of protein associations remains limited. We therefore developed a new approach that detects protein associations from identifications obtained after re-processing of large-scale, public mass spectrometry-based proteomics data...
October 13, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/29040674/evaluation-of-apoptotic-and-autophagic-related-protein-expressions-before-and-after-in-vitro-maturation-of-fresh-slow-frozen-and-vitrified-pre-pubertal-mouse-testicular-tissue
#16
L Dumont, F Chalmel, A Oblette, B Berby, A Rives, V Duchesne, C Rondanino, N Rives
STUDY QUESTION: Do freezing and in vitro culture procedures enhance the expression of proteins involved in apoptotic or autophagic pathways in murine pre-pubertal testicular tissue? SUMMARY ANSWER: In vitro maturation strongly modified apoptosis- and autophagy-related relative protein levels in mice testicular tissue whereas the impact of cryopreservation procedures was minimal at the end of the culture. WHAT IS KNOWN ALREADY: In vitro spermatogenesis remains a challenging technical issue as it imposes to find a very close balance between survival and death of germ cell natural precursors (i...
October 11, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29038038/global-proteome-and-phosphoproteome-dynamics-indicate-novel-mechanisms-of-vitamin-c-induced-dormancy-in-mycobacterium-smegmatis
#17
Claudia Albeldas, Naadir Ganief, Bridget Calder, Kehilwe C Nakedi, Shaun Garnett, Andrew J M Nel, Jonathan M Blackburn, Nelson C Soares
Vitamin C has been found to affect mycobacteria in multiple ways, including increasing susceptibility to antimicrobial drugs, inducing dormancy, and having a bactericidal effect. However, the regulatory events mediating vitamin C related adaptations remain largely elusive. Ser/Thr/Tyr protein phosphorylation plays an important regulatory role in mycobacteria, contributing to environmental adaptation, including dormancy and drug resistance. This study utilised the model organism, Mycobacterium smegmatis, and TiO2 phosphopeptide enrichment combined with mass spectrometry-based proteomics methods to elucidate the mycobacterial signalling and regulatory response to sub-lethal concentrations of vitamin C...
October 13, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29037750/environmental-toxicology-and-omics-a-question-of-sex
#18
Xuefang Liang, April Feswick, Denina Simmons, Christopher J Martyniuk
Molecular initiating events and downstream transcriptional/proteomic responses provide valuable information for adverse outcome pathways, which can be used predict the effects of chemicals on physiological systems. There has been a paucity of research that addresses sex-specific expression profiling in toxicology and due to cost, time, and logistical considerations, sex as a variable has not been widely considered. In response to this deficiency, federal agencies in the United States, Canada, and Europe have highlighted the importance of including sex as a variable in scientific investigations...
October 13, 2017: Journal of Proteomics
https://www.readbyqxmd.com/read/29037594/epigenetic-modification-of-microrna-200b-contributes-to-diabetic-vasculopathy
#19
Kanhaiya Singh, Durba Pal, Mithun Sinha, Subhadip Ghatak, Surya C Gnyawali, Savita Khanna, Sashwati Roy, Chandan K Sen
Hyperglycemia (HG) induces genome-wide cytosine demethylation. Our previous work recognized miR-200b as a critical angiomiR, which must be transiently downregulated to initiate wound angiogenesis. Under HG, miR-200b downregulation is not responsive to injury. Here, we demonstrate that HG may drive vasculopathy by epigenetic modification of a miR promoter. In human microvascular endothelial cells (HMECs), HG also lowered DNA methyltransferases (DNMT-1 and DNMT-3A) and compromised endothelial function as manifested by diminished endothelial nitric oxide (eNOS), lowered LDL uptake, impaired Matrigel tube formation, lower NO production, and compromised VE-cadherin expression...
September 12, 2017: Molecular Therapy: the Journal of the American Society of Gene Therapy
https://www.readbyqxmd.com/read/29036721/blood-based-protein-changes-in-childhood-are-associated-with-increased-risk-for-later-psychotic-disorder-evidence-from-a-nested-case-control-study-of-the-alspac-longitudinal-birth-cohort
#20
Jane A English, Lorna M Lopez, Aoife O'Gorman, Melanie Föcking, Magdalena Hryniewiecka, Caitriona Scaife, Sophie Sabherwal, Kieran Wynne, Patrick Dicker, Bart P F Rutten, Glynn Lewis, Stanley Zammit, Mary Cannon, Gerard Cagney, David R Cotter
The identification of early biological changes associated with the psychotic disorder (PD) is important as it may provide clues to the underlying pathophysiological mechanisms. We undertook the first proteomic profiling of blood plasma samples of children who later develop a PD. Participants were recruited from the UK Avon Longitudinal Study of Parents and Children (ALSPAC) cohort who also participated in psychiatric assessment interviews at age 18. Protein expression levels at age 11 were compared between individuals who developed PD at age 18 (n = 37) with population-based age-matched controls (n = 38)...
July 27, 2017: Schizophrenia Bulletin
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