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https://www.readbyqxmd.com/read/28821617/janus-kinase-3-regulates-adherens-junction-and-epithelial-mesenchymal-transition-through-beta-catenin
#1
Jayshree Mishra, Jugal Kishore Das, Narendra Kumar
Compromise in adherens junction (AJ) is associated with several chronic inflammatory diseases. Previously we reported that Janus kinase-3, a non-receptor tyrosine kinase plays a crucial role in AJ formation through its interaction with beta-catenin. In this report, we characterize the structural determinants responsible for Jak3 interactions with beta-catenin and determine the functional implications of previously unknown tyrosine residues on beta-catenin phosphorylated by Jak3. We demonstrate that Jak3 auto-phosphorylation was the rate- limiting step during Jak3 trans-phosphorylation of beta-catenin where Jak3 directly phosphorylated three tyrosine residues viz...
August 17, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28819764/secad-and-egf-levels-increased-in-urine-of-non-ferrous-metal-workers-and-medium-of-uroepithelial-cell-line-treated-by-arsenic
#2
Jieyu Liu, Peiyu Jin, Shengnan Liu, Fei Wang, Xiaoyan Wang, Li Yang, Shuhua Xi
Inorganic arsenic (iAs) is a carcinogen and could increase the risks of bladder, lung, and skin cancer. Mining and smelting of non-ferrous metals are common occupational arsenic exposures. In this study, 125 individuals working in non-ferrous metal smelting plants were separated into two groups according to urinary total arsenic (TAs) levels: group 1, TAs < 100 μg/g Cr; group 2, TAs ≥ 100 μg/g Cr. Demographic characteristics of participants were obtained by questionnaire interview. Levels of E-cadherin soluble ectodomain fragment (sEcad) and epidermal growth factor (EGF) in workers urine were determined by ELISA test...
August 17, 2017: Biological Trace Element Research
https://www.readbyqxmd.com/read/28814502/selective-regulation-of-clathrin-mediated-egfr-signaling-and-endocytosis-by-phospholipase-c-and-calcium
#3
Ralph Christian Delos Santos, Stephen Bautista, Stefanie Lucarelli, Leslie N Bone, Roya M Dayam, John Abousawan, Roberto J Botelho, Costin N Antonescu
Clathrin-mediated endocytosis is a major regulator of cell surface protein internalization. Clathrin and other proteins assemble into small invaginating structures at the plasma membrane termed clathrin-coated pits (CCPs) that mediate vesicle formation. In addition, epidermal growth factor (EGF) receptor (EGFR) signaling is regulated by its accumulation within CCPs. Given the diversity of proteins regulated by clathrin-mediated endocytosis, how this process may distinctly regulate specific receptors is a key question...
August 16, 2017: Molecular Biology of the Cell
https://www.readbyqxmd.com/read/28811301/topically-applied-carvedilol-attenuates-solar-ultraviolet-radiation-induced-skin-carcinogenesis
#4
Kevin M Huang, Sherry Liang, Steven Yeung, Etuajie Oiyemhonlan, Kristan H Cleveland, Cyrus Parsa, Robert Orlando, Frank L Meyskens, Bradley Andresen, Ying Huang
In previous studies, the β-blocker carvedilol inhibited EGF-induced epidermal cell transformation and chemical carcinogen-induced mouse skin hyperplasia. As exposure to ultraviolet (UV) radiation leads to skin cancer, the present study examined whether carvedilol can prevent UV-induced carcinogenesis. Carvedilol absorbs UV like a sunscreen; thus, to separate pharmacological from sunscreen effects, 4-hydroxycarbazole (4-OHC), which absorbs UV to the same degree as carvedilol, served as control. JB6 P+ cells, an established epidermal model for studying tumor promotion, were used for evaluating the effect of carvedilol on UV-induced neoplastic transformation...
August 15, 2017: Cancer Prevention Research
https://www.readbyqxmd.com/read/28810691/improvement-of-implantation-potential-in-mouse-blastocysts-derived-from-ivf-by-combined-treatment-with-prolactin-epidermal-growth-factor-and-4-hydroxyestradiol
#5
Miki Takeuchi, Misato Seki, Etsuko Furukawa, Akihito Takahashi, Kyosuke Saito, Mitsuru Kobayashi, Kenji Ezoe, Emiko Fukui, Midori Yoshizawa, Hiromichi Matsumoto
STUDY QUESTION: Can supplementation of medium with prolactin (PRL), epidermal growth factor (EGF) and 4-hydroxyestradiol (4-OH-E2) prior to embryo transfer improve implantation potential in mouse blastocysts derived from IVF? SUMMARY ANSWER: Combined treatment with PRL, EGF and 4-OH-E2 improves mouse blastocyst implantation rates, while alone, each factor is ineffective. WHAT IS KNOWN ALREADY: Blastocyst dormancy during delayed implantation caused by ovariectomy is maintained by continued progesterone treatment in mice, and estrogen injection rapidly activates blastocysts to implantation-induced status in vivo...
August 1, 2017: Molecular Human Reproduction
https://www.readbyqxmd.com/read/28808215/ginkgol-c17-1-inhibits-tumor-growth-by-blunting-the-egf-pi3k-akt-signaling-pathway
#6
Yueying Li, Jun Liu, Xiaoming Yang, Yan Dong, Yali Liu, Min Chen
Ginkgol C17:1 has been shown to inhibit apoptosis and migration of cancer cells, but the underlying mechanisms are not fully elucidated. In this study, we explored whether the inhibitory effects of Ginkgol C17:1 were associated with epidermal growth factor receptor (EGFR) and PI3K/Akt signaling. The results showed that EGF treatment increased the phosphorylation of EGFR, PI3K, Akt, mTOR and NF-kB, and also enhanced the proliferation, migration and invasion of HepG2 cells. Ginkgol C17:1 dose-dependently inhibited EGF-induced phosphorylation/activation of all the key components including EGFR, PI3K, Akt, mTOR and NF-kB, leading to a significant reduction either of proliferation or migration and invasion of HepG2 cells...
January 19, 2017: Journal of Biomedical Research
https://www.readbyqxmd.com/read/28807821/popdc1-is-suppressed-in-human-breast-cancer-tissues-and-is-negatively-regulated-by-egfr-in-breast-cancer-cell-lines
#7
Johanna Ndamwena Amunjela, Steven John Tucker
Breast cancer molecular heterogeneity has resulted in disparities in therapeutic response and targeting of molecular subtypes of breast cancer. This necessitates identification and validation of novel therapeutic targets for breast cancer treatment. Suppression of Popeye domain-containing (POPDC) proteins is hypothesized to promote malignant cell behaviour and poor clinical outcomes in various cancers. We aimed to determine whether POPDC proteins are suppressed in human ductal carcinoma tissues and if this correlates to clinical progression and Her2 status...
August 12, 2017: Cancer Letters
https://www.readbyqxmd.com/read/28799757/immobilization-of-growth-factors-to-collagen-surfaces-using-visible-light
#8
Gabriella Maria Fernandes Cunha, Hyun Jong Lee, Alisha Kumar, Alexander Kreymerman, Sarah Christine Heilshorn, David Myung
In the treatment of traumatic injuries, burns, and ulcers of the eye, inadequate epithelial tissue healing remains a major challenge. Wound healing is a complex process involving the temporal and spatial interplay between cells and their extracellular milieu. It can be impaired by a variety of causes including infection, poor circulation, loss of critical cells and/or proteins, and a deficiency in normal neural signaling (e.g. neurotrophic ulcers). Ocular anatomy is particularly vulnerable to lasting morbidity from delayed healing, whether it be scarring or perforation of the cornea, destruction of the conjunctival mucous membrane, or cicatricial changes to the eyelids and surrounding skin...
August 11, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28799616/quantitative-investigation-of-mda-mb-231-breast-cancer-cell-motility-dependence-on-epidermal-growth-factor-concentration-and-its-gradient
#9
Tanzila Islam, Haluk Resat
Enhanced cell motility is one of the primary features of cancer. Accumulated evidence demonstrates that Epidermal Growth Factor Receptor (EGFR) mediated pathways play an important role in breast cancer cell proliferation and migration. We have quantified the MDA-MB-231 breast cancer cell migration in response to the stimulation of EGFR pathways with their ligand EGF to determine how the cell motility of MDA-MB-231 cells depends on the ligand concentration and gradient. Analysis at the single cell level combined with mathematical modeling and the ability to vary the ligand concentration and gradients locally using microfluidic devices allowed us to separate the unique contributions of ligand concentration and ligand gradient to cell motility...
August 11, 2017: Molecular BioSystems
https://www.readbyqxmd.com/read/28799567/identification-of-breast-cancer-mechanism-based-on-weighted-gene-coexpression-network-analysis
#10
X Guo, H Xiao, S Guo, L Dong, J Chen
Our gene expression-profiling analysis aimed to explain the mechanism of breast cancer development by identifying key pathways and constructing networks of related transcription factors (TFs) and microRNAs (miRNAs) in breast cancer tissues. Gene expression profiles of normal and breast cancer tissues were downloaded to identify differentially expressed genes (DEGs). Coexpression modules were explored using weighted gene coexpression network analysis (WGCNA). Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed to discover the enriched functionally associated gene groups and define pathways in breast cancer, respectively...
August 11, 2017: Cancer Gene Therapy
https://www.readbyqxmd.com/read/28798239/interaction-of-munc18c-and-syntaxin4-facilitates-invadopodium-formation-and-extracellular-matrix-invasion-of-tumour-cells
#11
Megan I Brasher, David M Martynowicz, Olivia R Grafinger, Andrea Hucik, Emma Shanks-Skinner, James Uniacke, Marc G Coppolino
Tumor cell invasion involves targeted localization of proteins required for interactions of the extracellular matrix (ECM) and for proteolysis. The localization of many proteins during these cell-ECM interactions relies on membrane trafficking mediated in part by soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs). The SNARE protein syntaxin4 (Stx4) is involved in the formation of invasive structures called invadopodia; however, it is unclear how Stx4 function is regulated during tumor cell invasion...
August 10, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28797129/differential-effects-of-amnion-and-chorion-membrane-extracts-on-osteoblast-like-cells-due-to-the-different-growth-factor-composition-of-the-extracts
#12
Yoon Young Go, Sung Eun Kim, Geum Joon Cho, Sung-Won Chae, Jae-Jun Song
Human amniotic membrane extracts contain numerous growth factors and bioactive substances. However, osteogenic effects of amnion and chorion membrane extracts (AME and CME, respectively) on osteoblasts are unclear. In this study, we explored the ability of AME and CME to promote the osteogenic differentiation of osteoblast-like MG-63 cells. MG-63 cells were cultured in osteogenic induction medium (OIM) with or without exogenous AME and CME. CME enhanced the osteogenic differentiation of MG-63 cells compared with AME, as indicated by increased mineralization; alkaline phosphatase activity; and mRNA expression of osteogenic marker genes encoding integrin-binding sialoprotein (IBSP), RUNX2, OSTERIX, and osteocalcin (OCN)...
2017: PloS One
https://www.readbyqxmd.com/read/28795885/the-influence-of-amniotic-membrane-extracts-on-cell-growth-depends-on-the-part-of-membrane-and-childbirth-mode-selected-a-proof-of-concept-study
#13
M Litwiniuk, M Radowicka, A Krejner, T Grzela
OBJECTIVE: The amniotic membrane (AM) is a rich source of biologically active factors, important for wound healing and is widely used in various clinical applications, including tissue engineering, reconstructive surgery and wound management. The aim of the present proof-of-concept study was to assess the influence of amniotic membrane extracts on in vitro proliferation of main cells involved in tissue regeneration. The assessment was done in regards to the content of selected biologically active factors in amniotic membrane extracts...
August 2, 2017: Journal of Wound Care
https://www.readbyqxmd.com/read/28792970/targeting-hepatic-heparin-binding-egf-like-growth-factor-hb-egf-induces-anti-hyperlipidemia-leading-to-reduction-of-angiotensin-ii-induced-aneurysm-development
#14
Seonwook Kim, Lihua Yang, Seongu Kim, Richard G Lee, Mark J Graham, Judith A Berliner, Aldons J Lusis, Lei Cai, Ryan E Temel, Debra L Rateri, Sangderk Lee
OBJECTIVE: The upregulated expression of heparin binding EGF-like growth factor (HB-EGF) in the vessel and circulation is associated with risk of cardiovascular disease. In this study, we tested the effects of HB-EGF targeting using HB-EGF-specific antisense oligonucleotide (ASO) on the development of aortic aneurysm in a mouse aneurysm model. APPROACH AND RESULTS: Low-density lipoprotein receptor (LDLR) deficient mice (male, 16 weeks of age) were injected with control and HB-EGF ASOs for 10 weeks...
2017: PloS One
https://www.readbyqxmd.com/read/28791994/-expression-of-epidermal-growth-factor-receptor-in-patients-with-insulin-resistance-and-seborrheic-keratomas
#15
V A Smolyannikova, A K Alexandrova
AIM: to study the expression of epidermal growth factor receptor (EGFR) in patients with seborrheic keratomas (SK) and insulin resistance (type 2 diabetes mellitus (DM2)) and in those without concomitant carbohydrate metabolic disturbances. SUBJECTS AND METHODS: 80 patients with SK were examined. According to the presence or absence of DM2, the patients were divided into 2 groups: 1) 40 patients with concomitant DM2; 2) 40 people without carbohydrate metabolic disturbances...
2017: Arkhiv Patologii
https://www.readbyqxmd.com/read/28791645/measurement-of-epidermal-growth-factor-receptor-derived-signals-within-plasma-membrane-clathrin-structures
#16
Stefanie Lucarelli, Ralph Christian Delos Santos, Costin N Antonescu
The epidermal growth factor (EGF) receptor (EGFR) is an important regulator of cell growth, proliferation, survival, migration, and metabolism. EGF binding to EGFR triggers the activation of the receptor's intrinsic kinase activity, in turn eliciting the recruitment of many secondary signaling proteins and activation of downstream signals, such as the activation of phosphatidylinositol-3-kinase (PI3K) and Akt, a process requiring the phosphorylation of Gab1. While the identity of many signals that can be activated by EGFR has been revealed, how the spatiotemporal organization of EGFR signaling within cells controls receptor outcome remains poorly understood...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28791644/analysis-of-constitutive-egfr-signaling-regulating-irf3-transcriptional-activity-in-cancer-cells
#17
Gao Guo, Ke Gong, Amyn A Habib
Epidermal growth factor receptor (EGFR) plays an important role in various types of human cancers. Overexpression of EGFR leads to a constitutive tyrosine phosphorylation of multiple tyrosine residues in the EGFR. Recently, we have demonstrated that overexpressed EGFR oscillates between two distinct and mutually exclusive modes of signaling depending on the presence or absence of ligand. EGFR constitutively activates transcription factor IRF3, which results in transcription of its target genes. Addition of EGF causes a loss of IRF3 transcriptional activity and activation of canonical signaling pathways such as ERK...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28791643/cell-cycle-synchronization-of-hela-cells-to-assay-egfr-pathway-activation
#18
Ping Wee, Zhixiang Wang
Progression through the cell cycle causes changes in the cell's signaling pathways that can alter EGFR signal transduction. Here, we describe drug-derived protocols to synchronize HeLa cells in various phases of the cell cycle, including G1 phase, S phase, G2 phase, and mitosis, specifically in the mitotic stages of prometaphase, metaphase, and anaphase/telophase. The synchronization procedures are designed to allow synchronized cells to be treated for EGF and collected for the purpose of Western blotting for EGFR signal transduction components...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28791640/study-of-egfr-signaling-endocytosis-by-site-directed-mutagenesis
#19
Qian Wang, Zhixiang Wang
As an essential tool in molecular biology and biochemistry, in vitro mutagenesis is important for mechanistic and functional studies of protein at the cellular level. Through site-directed substitution, insertion, or deletion of potential function residues, we may evaluate the specific biological role of proteins and understand the underlying mechanism. EGF ligand binding will activate EGFR and initiate signaling transduction for cell proliferation, differentiation, and survival; meanwhile, it will also induce the EGFR endocytosis for degradation to maintain cellular homeostasis...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28791638/activation-of-endosome-associated-inert-egf-receptor-following-internalization
#20
Yi Wang, Sukhmani Billing, Zhixiang Wang
Our current understanding of endosomal signaling is incomplete given our inability to generate endosomal signals in isolation from plasma membrane signals. We present a novel method to specifically activate epidermal growth factor (EGF) receptor (EGFR) following its internalization into endosomes. This method will allow us to address questions such as whether endosomal signaling is sufficient to activate signal transduction pathways and produce biological responses. In this method, the cells are treated with EGF in the presence of AG1478, a specific EGFR tyrosine kinase inhibitor, and monensin, which blocks recycling of EGFR...
2017: Methods in Molecular Biology
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