You-Ran Luo, Tada-Aki Kudo, Kanako Tominami, Satoshi Izumi, Takakuni Tanaka, Yohei Hayashi, Takuya Noguchi, Atsushi Matsuzawa, Junichi Nakai, Guang Hong, Hang Wang
This study evaluated the mechanism of temperature-controlled repeated thermal stimulation (TRTS)-mediated neuronal differentiation. We assessed the effect of SP600125, a c-Jun N-terminal kinase (JNK) inhibitor, on neuronal differentiation of rat PC12-P1F1 cells, which can differentiate into neuron-like cells by exposure to TRTS or neurotrophic factors, including bone morphogenetic protein (BMP) 4. We evaluated neuritogenesis by incubating the cells under conditions of TRTS and/or SP600125. Cotreatment with SP600125 significantly enhanced TRTS-mediated neuritogenesis, whereas that with other selective mitogen-activated protein kinase (MAPK) inhibitors did not-e...
December 9, 2022: International Journal of Molecular Sciences