keyword
MENU ▼
Read by QxMD icon Read
search

sialic

keyword
https://www.readbyqxmd.com/read/28526748/the-sialate-o-acetylesterase-esta-from-gut-bacteroidetes-species-enables-sialidase-mediated-cross-species-foraging-of-9-o-acetylated-sialoglycans
#1
Lloyd S Robinson, Warren G Lewis, Amanda L Lewis
The gut harbors many symbiotic, commensal, and pathogenic microbes that engage in the breakdown and metabolism of host carbohydrates. Sialic acids are prominent outermost carbohydrates on mucins and protect underlying glycan chains from enzymatic degradation. Sialidases produced by some members of the colonic microbiota have been shown to promote the expansion of several potential pathogens (e.g. Clostridium difficile, Salmonella, Escherichia coli) that do not produce sialidases. O-acetyl ester modifications of sialic acids help resist the action of many sialidases and are found at high levels in the mammalian colon...
May 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28524373/sialic-acids-regulate-microvessel-permeability-revealed-by-novel-in-vivo-studies-of-endothelial-glycocalyx-structure-and-function
#2
Kai Betteridge, Kenton Arkill, Chris Neal, Steve Harper, Becky Foster, Simon Satchell, David Bates, Andy Salmon
The endothelial glycocalyx forms a continuous coat over the luminal surface of all vessels, and regulates multiple vascular functions. The contribution of individual components of the endothelial glycocalyx to one critical vascular function, microvascular permeability, remains unclear. We developed novel, real-time, paired methodologies to study the contribution of sialic acids within the endothelial glycocalyx to the structural and functional permeability properties of the same microvessel in vivo. Single perfused rat mesenteric microvessels were perfused with fluorescent endothelial cell membrane and glycocalyx labels, and imaged with confocal microscopy...
May 19, 2017: Journal of Physiology
https://www.readbyqxmd.com/read/28524185/erythrocytes-based-quartz-crystal-microbalance-cytosensor-for-in-situ-detection-of-cell-surface-sialic-acid
#3
Xiaojuan Yang, Lin Zhou, Yan Hao, Bin Zhou, Peihui Yang
An erythrocytes (RBCs) cytosensor was first fabricated for in situ analysis of sialic acid (SA) on the cell surface based on a quartz crystal microbalance (QCM). RBCs, as a recognition element, were immobilized on a concanavalin A (ConA)-modified gold chip through the specific recognition between ConA and mannose on the cell surface. 4-Aminobenzeneboronic acid (APBA)-functionalized gold nanoparticles (AuNPs/APBA) were used as a signal amplification nanoprobe for labeling SA on the surface of RBCs. Compared to that of APBA, the frequency response of the cytosensor could be significantly enhanced 18-fold by using a AuNPs/APBA nanoprobe...
May 19, 2017: Analyst
https://www.readbyqxmd.com/read/28521959/cancer-cell-death-induced-by-nanomagnetolectin
#4
Dina M M AlSadek, Haitham A Badr, Tamer A Al-Shafie, Sabry M El-Bahr, Motawa E El-Houseini, Leyla B Djansugurova, Chen-Zhong Li, Hafiz Ahmed
Magnetic nanoparticles represent a new paradigm for molecular targeting therapy in cancer. However, the transformative targeting potential of magnetic nanoparticles has been stymied by a key obstacle-safe delivery to specified target cells in vivo. As cancer cells grow under nutrient deprivation and hypoxic conditions and decorate cell surface with excessive sialoglycans, sialic acid binding lectins might be suitable for targeting cancer cells in vivo. Here we explore the potential of magnetic nanoparticles functionalized with wheat germ lectin (WGA) conjugate, so-called nanomagnetolectin, as apoptotic targetable agents for prostate cancer...
May 10, 2017: European Journal of Cell Biology
https://www.readbyqxmd.com/read/28518173/simultaneous-quantification-of-n-and-o-glycans-using-a-solid-phase-method
#5
Shuang Yang, Yingwei Hu, Lori Sokoll, Hui Zhang
Glycosylation has a pivotal role in a diverse range of biological activities, modulating the structure and function of proteins. Glycogens coupled to the nitrogen atom (N-linked) of asparagine side chains or to the oxygen atom (O-linked) of serine and threonine side chains represent the two major protein glycosylation forms. N-glycans can be released by glycosidases, whereas O-glycans are often cleaved by chemical reaction. However, it is challenging to combine these enzymatic and chemical reactions in order to analyze both N- and O-glycans...
June 2017: Nature Protocols
https://www.readbyqxmd.com/read/28514082/universal-label-free-in-process-quantification-of-influenza-virus-like-particles
#6
Sofia B Carvalho, Mafalda G Moleirinho, David Wheatley, John Welsh, René Gantier, Paula M Alves, Cristina Peixoto, Manuel J T Carrondo
Virus-like particles (VLPs) are becoming established as vaccines, in particular for influenza pandemics, increasing the interest in the development of VLPs manufacturing bioprocess. However, for complex VLPs, the analytical tools used for quantification are not yet able to keep up with the bioprocess progress. Currently, quantification for Influenza relies on traditional methods: hemagglutination assay or Single Radial Immunodiffusion. These analytical technologies are time-consuming, cumbersome, and not supportive of efficient downstream process development and monitoring...
May 17, 2017: Biotechnology Journal
https://www.readbyqxmd.com/read/28512676/different-fermentation-processes-produced-variants-of-an-anti-cd52-monoclonal-antibody-that-have-divergent-in-vitro-and-in-vivo-characteristics
#7
Chao Zhuang, Chen Zheng, Yantian Chen, Zheng Huang, Yanchao Wang, Qiang Fu, Chen Zeng, Tong Wu, Liming Yang, Nianmin Qi
The anti-CD52 antibody has already been approved for the treatment of patients with resistant chronic lymphocytic leukemia, relapsing-remitting multiple sclerosis, and has demonstrable efficacy against stem cell transplantation rejection. A CHO cell line expressing a humanized anti-CD52 monoclonal antibody (mAb-TH) was cultivated in both fed-batch and perfusion modes, and then purified. The critical quality attributes of these mAb variants were characterized and the pharmacokinetics (PK) properties were investigated...
May 16, 2017: Applied Microbiology and Biotechnology
https://www.readbyqxmd.com/read/28511503/evaluation-of-total-and-lipid-bound-sialic-acid-in-serum-in-oral-leukoplakia
#8
Karthik Krishnan, Sivapathasundharam Balasundaram
INTRODUCTION: Oral cancer has become the most common cause of cancer related mortality which may be preceded by oral potentially malignant disorders. Altered glycosylation of glycoconjugates, such as sialic acid are one of the most important molecular changes accompanied during malignant transformation in precancerous lesions like Oral Leukoplakia (OL), and correlating them histopathologically with grades of epithelial dysplasia which will serve clinical significance. AIM: The aim of this study was to evaluate the significance of serum Total Sialic Acid (TSA) and serum Lipid Bound Sialic Acid (LSA) as a prognostic serum marker in Oral Leukoplakia...
March 2017: Journal of Clinical and Diagnostic Research: JCDR
https://www.readbyqxmd.com/read/28509534/analysis-of-three-epoetin-alpha-products-by-lc-and-lc-ms-indicates-differences-in-glycosylation-critical-quality-attributes-including-sialic-acid-content
#9
Rebecca I Thomson, Richard Andrew Gardner, Katja Strohfeldt, Daryl L Fernandes, Graham Philip Stafford, Daniel I R Spencer, Helen M I Osborn
Erythropoietin (EPO) is one of the main treatments for anaemic patients, greatly improving their quality of life. In this study, biosimilars Binocrit and a development product, called here CIGB-EPO, were compared to the originator product, Eprex. All three are epoetin alpha products, reputed to have similar glycosylation profiles. The quality, safety and efficacy of this biotherapeutic depend on the following glycosylation critical quality attributes (GCQAs): sialylation, N-glycolyl-neuraminic acid (Neu5Gc) content, branching, N-acetyl-lactosamine (LacNAc) extensions and, arguably, O-acetylation pattern...
May 16, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28509371/quantitative-assessment-of-sialo-glycoproteins-and-n-glycans-during-cardiomyogenic-differentiation-of-human-induced-pluripotent-stem-cells
#10
Sarah Anna Konze, Samanta Cajic, Astrid Oberbeck, René Hennig, Andreas Pich, Erdmann Rapp, Falk Buettner
Human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) may be used in regenerative medicine for individualized tissue transplants in future. For application in patients, the generated CMs have to be highly pure and well characterized. To overcome the prevalent scarcity of CM-specific markers, we quantitatively assessed cell surface exposed sialo-glycoproteins and N-glycans of hiPSCs, CM progenitors and CMs derived thereof. Applying a combination of metabolic labeling and specific sialo-glycoprotein capture, we could highly enrich and quantify membrane proteins during cardiomyogenic differentiation...
May 16, 2017: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/28509333/altered-glycan-accessibility-on-native-immunoglobulin-g-complexes-in-early-rheumatoid-arthritis-and-its-changes-during-therapy
#11
Judith Stümer, Mona H C Biermann, Jasmin Knopf, Iryna Magorivska, Alf Kastbom, Anna Svärd, Christina Janko, Rostyslav Bilyy, Georg Schett, Christopher Sjöwall, Martin Herrmann, Luis E Muñoz
The goal of this study was to investigate the glycosylation profile of native IgG present in serum immune complexes in patients with rheumatoid arthritis (RA). To accomplish this, lectin binding assays, detecting the accessibility of glycans present on IgG-containing immune complexes by biotinylated lectins were employed. Lectins capturing fucosyl residues (AAL), fucosylated tri-mannose N-glycan core sites (LCA), terminal sialic acid residues (SNA) and O-glycosidically-linked galactose/N-acetylgalactosamine (GalNac-L) were used...
May 16, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28505427/modification-of-sialic-acids-on-solid-phase-accurate-characterization-of-protein-sialylation
#12
Shuang Yang, Lei Zhang, Stefani N Thomas, Yingwei Hu, Shuwei Li, John F Cipollo, Hui Zhang
Sialic acids play many important roles in several physiological and pathological processes, including cancers, infection and blood diseases. Sialic acids are fragile and prone to fragmentation under electrospray ionization and matrix-assisted laser desorption/ionization. It is crucial to modify sialic acids for qualitative and quantitative identification of their change in abundance in complex biological samples. Permethylation is a method of choice for sialic acid stabilization, but the harsh conditions during permethylation may lead to the decomposition of O-acetyl groups...
May 15, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28505249/sialic-acid-deficiency-is-associated-with-oxidative-stress-leading-to-muscle-atrophy-and-weakness-in-the-gne-myopathy
#13
Anna Cho, May Christine V Malicdan, Miho Miyakawa, Ikuya Nonaka, Ichizo Nishino, Satoru Noguchi
Sialic acids are monosaccharides found in terminal sugar chains of cell surfaces and proteins; they have various biological functions and have been implicated in health and disease. Genetic defects of the GNE gene which encodes a critical bifunctional enzyme for sialic acid biosynthesis, lead to GNE myopathy, a disease manifesting with progressive muscle atrophy and weakness. The likely mechanism of disease is a lack of sialic acids. There remains, however, an unexplained link between hyposialylation and the muscle atrophy and weakness...
May 13, 2017: Human Molecular Genetics
https://www.readbyqxmd.com/read/28502901/neuraminidase-activity-of-blue-eye-disease-porcine-rubulavirus-specificity-affinity-and-inhibition-studies
#14
Gerardo Santos-López, María T Borraz-Argüello, Luis Márquez-Domínguez, Juan Carlos Flores-Alonso, Humberto Ramírez-Mendoza, Bernard Priem, Sébastien Fort, Verónica Vallejo-Ruiz, Julio Reyes-Leyva, Irma Herrera-Camacho
Porcine rubulavirus (PorPV), also known as La Piedad Michoacan Virus (LPMV) causes encephalitis and reproductive failure in newborn and adult pigs, respectively. The hemagglutinin-neuraminidase (HN) glycoprotein is the most exposed and antigenic of the virus proteins. HN plays central roles in PorPV infection; i.e., it recognizes sialic acid-containing cell receptors that mediate virus attachment and penetration; in addition, its neuraminidase (sialic acid releasing) activity has been proposed as a virulence factor...
May 6, 2017: Research in Veterinary Science
https://www.readbyqxmd.com/read/28501716/cross-protection-in-neisseria-meningitidis-serogroups-y-and-w-polysaccharides-a-comparative-conformational-analysis
#15
Michelle M Kuttel, Zaheer Timol, Neil Ravenscroft
The capsular polysaccharide is the main virulence factor in meningococcus. The capsular polysaccharides for meningococcal serogroups Y and W are almost identical polymers of hexose-sialic acid, suggesting the possibility of cross-protection between group Y and W vaccines. However, early studies indicated that they elicit different levels of cross-protection. Here we explore the conformations of the meningococcal Y and W polysaccharides with molecular dynamics simulations of three repeating unit oligosaccharide strands...
May 10, 2017: Carbohydrate Research
https://www.readbyqxmd.com/read/28500071/activated-microglia-desialylate-and-phagocytose-cells-via-neuraminidase-galectin-3-and-mer-tyrosine-kinase
#16
Koji Nomura, Anna Vilalta, David H Allendorf, Tamara C Hornik, Guy C Brown
Activated microglia can phagocytose dying, stressed, or excess neurons and synapses via the phagocytic receptor Mer tyrosine kinase (MerTK). Galectin-3 (Gal-3) can cross-link surface glycoproteins by binding galactose residues that are normally hidden below terminal sialic acid residues. Gal-3 was recently reported to opsonize cells via activating MerTK. We found that LPS-activated BV-2 microglia rapidly released Gal-3, which was blocked by calcineurin inhibitors. Gal-3 bound to MerTK on microglia and to stressed PC12 (neuron-like) cells, and it increased microglial phagocytosis of PC12 cells or primary neurons, which was blocked by inhibition of MerTK...
May 12, 2017: Journal of Immunology: Official Journal of the American Association of Immunologists
https://www.readbyqxmd.com/read/28499541/sequence-analysis-of-haemagglutinin-and-neuraminidase-of-h1n1-strain-from-a-patient-coinfected-with-mycobacterium-tuberculosis
#17
Ahmed N Alghamdi, Mohammad E Mahfouz, Fahd A Hamdi, Daifullah Al Aboud, Tawfiq Z Al-Laylah, Mohammed I Alotaibi, Khalid W A Al-Thomali, Ahmed S Abdel-Moneim
The 2009 H1N1 pandemic (H1N1pdm09) was associated with a considerable influenza-related morbidity and mortality. Among the complications, Mycobacterial tuberculosis was recorded as a coinfection with influenza in rare cases. The full-length sequences of the viral haemagglutinin and neuraminidase of H1N1pdm09 influenza A virus were analyzed from a recently infected patient. The patient was chronically infected with Mycobacterium tuberculosis. Molecular modelling and in-silico docking of the virus, and other selected strains with the drug oseltamivir were conducted and compared...
May 9, 2017: Molecular and Cellular Probes
https://www.readbyqxmd.com/read/28496342/sialylation-facilitates-self-assembly-of-3d-multicellular-prostaspheres-by-using-cyclo-rgdfk-tpp-peptide
#18
Sabah Haq, Vanessa Samuel, Fiona Haxho, Roman Akasov, Maria Leko, Sergey V Burov, Elena Markvicheva, Myron R Szewczuk
BACKGROUND: Prostaspheres-based three dimensional (3D) culture models have provided insight into prostate cancer (PCa) biology, highlighting the importance of cell-cell interactions and the extracellular matrix (EMC) in the tumor microenvironment. Although these 3D classical spheroid platforms provide a significant advance over 2D models mimicking in vivo tumors, the limitations involve no control of assembly and structure with only limited spatial or glandular organization. Here, matrix-free prostaspheres from human metastatic prostate carcinoma PC3 and DU145 cell lines and their respective gemcitabine resistant (GemR) variants were generated by using cyclic Arg-Gly-Asp-D-Phe-Lys peptide modified with 4-carboxybutyl-triphenylphosphonium bromide (cyclo-RGDfK(TPP))...
2017: OncoTargets and Therapy
https://www.readbyqxmd.com/read/28495784/implication-of-sialidases-in-salmonella-infection-genome-release-of-sialidase-knockout-strains-from-salmonella-enterica-serovar-typhimurium-lt2
#19
Narine Arabyan, Allison M Weis, Bihua C Huang, Bart C Weimer
Sialidases, which are widely distributed in nature, cleave the α-ketosidic bond of terminal sialic acid residue. These emerging virulence factors degrade the host glycan. We report here the release of seven sialidase and one sialic acid transporter deletion in Salmonella enterica serovar Typhimurium strain LT2, which are important in cellular invasion during infection.
May 11, 2017: Genome Announcements
https://www.readbyqxmd.com/read/28495148/analysis-of-urinary-psa-glycosylation-is-not-indicative-of-high-risk-prostate-cancer
#20
Sílvia Barrabés, Esther Llop, Montserrat Ferrer-Batallé, Manel Ramírez, Rosa N Aleixandre, Antoinette S Perry, Rafael de Llorens, Rosa Peracaula
The levels of core fucosylation and α2,3-linked sialic acid in serum Prostate Specific Antigen (PSA), using the lectins Pholiota squarrosa lectin (PhoSL) and Sambucus nigra agglutinin (SNA), can discriminate between Benign Prostatic Hyperplasia (BPH) and indolent prostate cancer (PCa) from aggressive PCa. In the present work we evaluated whether these glycosylation determinants could also be altered in urinary PSA obtained after digital rectal examination (DRE) and could also be useful for diagnosis determinations...
May 8, 2017: Clinica Chimica Acta; International Journal of Clinical Chemistry
keyword
keyword
78549
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"