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https://www.readbyqxmd.com/read/28646531/morin-a-novel-lxr%C3%AE-%C3%AE-dual-antagonist-has-potent-therapeutic-efficacy-for-nonalcoholic-fatty-liver-diseases
#1
Ming Gu, Yu Zhang, Chuhe Liu, Dongshan Wang, Li Feng, Shengjie Fan, Baican Yang, Qingchun Tong, Guang Ji, Cheng Huang
BACKGROUND AND PURPOSE: Morin is a natural occurring flavonoid in many dietary plants and has a wide range of beneficial effects on metabolism; however, the mechanism underlying its action remains elusive. EXPERIMENTAL APPROACH: Reporter assay and the Time-Resolved Fluorescence Resonance Energy Transfer assay were used to identify morin as a dual antagonist of LXRα and LXRβ. High fat-diet mixed with morin (100 mg/100 g diet) was administrated to high-fat diet-induced obese or LXRβ(-/-) mice combining with Western blot and RT-PCR analysis to evaluate the pharmacological effect and mechanism of morin treatment...
June 23, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/28644988/effect-of-marbling-on-volatile-generation-oral-breakdown-and-in-mouth-flavor-release-of-grilled-beef
#2
Damian Frank, Kornelia Kaczmarska, Janet Paterson, Udayasika Piyasiri, Robyn Warner
While the positive effect of intramuscular fat (IMF) on beef tenderness is well-established, its role in flavor generation and flavor release is less defined. To increase understanding, real-time volatile generation was monitored during grilling of beefsteaks (grass and grain-fed Angus and grass-fed Wagyu) with different amounts of IMF by proton transfer reaction mass spectrometry. Volatile concentration increased significantly (p<0.001) when the IMF was >~10%, but did not differ (p>0.05) at lower IMF levels (5...
June 15, 2017: Meat Science
https://www.readbyqxmd.com/read/28643865/characterization-and-biological-function-of-milk-derived-mirnas
#3
Regina Golan-Gerstl, Yaffa Elbaum Shiff, Vardit Lavi-Moshayoff, Daniel Schecter Dena Leshkowitz, Shimon Reif
SCOPE: Breastfeeding is associated with reduced risk of infection, immune mediated disorders, obesity and even cancer. Recently it was found that breast milk contains a variety of microRNAs (miRNAs) in the skim and fat layer that can be transferred to infants, and appear to play important roles in those biological functions. METHODS AND RESULTS: This study applied Next Generation Sequencing and quantitative real time PCR analysis to determine the miRNA expression profile of the skim and fat fraction of human, goat, and bovine milk as well as infant formulas...
June 23, 2017: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/28643006/complications-of-fat-transfer-for-breast-augmentation
#4
Paraskevas Kontoes, George Gounnaris
Autologous fat grafting is quite common for breast augmentations as well as for reconstructive breast surgery. Coleman has described the surgical technique of fat grafting. Fat is harvested, and after centrifugation and refinement, blunt infiltration cannulas are used to place the fat through small incisions. The grafted tissue is placed in small aliquots with each withdrawal of the cannula. In order to achieve an aesthetically pleasing contour of the breast, the fat should be layered into different levels from the chest wall to the skin...
June 22, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28637516/feeding-heat-oxidized-oil-to-dairy-cows-affects-milk-fat-nutritional-quality
#5
M Kaleem, F Enjalbert, Y Farizon, A Meynadier
Heating oil and oilseeds results in oxidation products that affect ruminal biohydrogenation of polyunsaturated fatty acids, altering milk fatty acids profile, and could be transferred to milk. An experiment was conducted to investigate the effects of oil heating on rumen and milk fatty acids profile and the transfer of oxidation products to milk. Sunflower oil was heated at 150°C for 15 h and given to lactating dairy cows in a 2×2 arrangement: two groups of two cows, equipped with a ruminal cannula and receiving two diets (containing either heated or unheated oil) during two experimental periods...
June 22, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28634702/rectus-abdominis-fat-transfer-raft-in-lipoabdominoplasty-a-new-technique-to-achieve-fitness-body-contour-in-patients-that-require-tummy-tuck
#6
Stefan Danilla
OBJECTIVE: To describe a new technique to achieve a fit-look in lipoabdominoplasty patients through liposculpture and rectus abdominis fat transfer (RAFT). METHODS: Patients with a body mass index under 26 and of both genders scheduled for abdominoplasty or body lift were selected for this procedure. Fat was transferred directly to the rectus abdominis muscle after flap elevation during lipoabdominoplasty. RESULTS: A total of 26 patients were operated on with this technique...
June 20, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28630339/ucp1-deficiency-causes-brown-fat-respiratory-chain-depletion-and-sensitizes-mitochondria-to-calcium-overload-induced-dysfunction
#7
Lawrence Kazak, Edward T Chouchani, Irina G Stavrovskaya, Gina Z Lu, Mark P Jedrychowski, Daniel F Egan, Manju Kumari, Xingxing Kong, Brian K Erickson, John Szpyt, Evan D Rosen, Michael P Murphy, Bruce S Kristal, Steven P Gygi, Bruce M Spiegelman
Brown adipose tissue (BAT) mitochondria exhibit high oxidative capacity and abundant expression of both electron transport chain components and uncoupling protein 1 (UCP1). UCP1 dissipates the mitochondrial proton motive force (Δp) generated by the respiratory chain and increases thermogenesis. Here we find that in mice genetically lacking UCP1, cold-induced activation of metabolism triggers innate immune signaling and markers of cell death in BAT. Moreover, global proteomic analysis reveals that this cascade induced by UCP1 deletion is associated with a dramatic reduction in electron transport chain abundance...
June 19, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28628501/comparison-of-donor-site-drainage-duration-and-seroma-rate-between-latissimus-dorsi-musculocutaneous-flaps-and-thoracodorsal-artery-perforator-flaps
#8
Masaki Arikawa, Shimpei Miyamoto, Masahide Fujiki, Takuya Higashino, Azusa Oshima, Minoru Sakuraba
INTRODUCTION: Donor site seroma formation and prolonged drainage duration are common complications of using latissimus dorsi musculocutaneous (LDMC) flaps. The present retrospective study aimed to investigate the effect of types of back flap harvest on drainage duration and seroma formation rates. METHODS: Adult patients (n = 155) who underwent reconstruction with a latissimus dorsi flap from January 2010 to October 2015 were included in the study, of which 54 patients underwent breast reconstruction with a pedicled LDMC flap (LD breast group), 80 patients underwent an LDMC flap transfer for purposes other than breast reconstruction (LD nonbreast group), and 21 patients underwent soft tissue reconstruction with a thoracodorsal artery perforator (TAP) flap (TAP group)...
June 16, 2017: Annals of Plastic Surgery
https://www.readbyqxmd.com/read/28626803/estimation-of-insulin-secretion-glucose-uptake-by-tissues-and-liver-handling-of-glucose-using-a-mathematical-model-of-glucose-insulin-homeostasis-in-lean-and-obese-mice
#9
Michael Brenner, Sakineh Esmaeili Mohsen Abadi, Ramin Balouchzadeh, H Felix Lee, Hoo Sang Ko, Michael Johns, Nehal Malik, Joshua J Lee, Guim Kwon
Destruction of the insulin-producing β-cells is the key determinant of diabetes mellitus regardless of their types. Due to their anatomical location within the islets of Langerhans scattered throughout the pancreas, it is difficult to monitor β-cell function and mass clinically. To this end, we propose to use a mathematical model of glucose-insulin homeostasis to estimate insulin secretion, glucose uptake by tissues, and hepatic handling of glucose. We applied the mathematical model by Lombarte et al. (2013) to compare various rate constants representing glucose-insulin homeostasis between lean (11% fat)- and high fat diet (HFD; 45% fat)-fed mice...
June 2017: Heliyon
https://www.readbyqxmd.com/read/28626094/-effect-of-trehalose-on-survival-rate-for-%C3%A2-fat-cells-after-cryopreservation
#10
Ying Deng, Shaoqian Liu, Hongju Xie, Fangfang Tang, Ming Li, Nian Chen
To explore effects of trehalose as a cryopreserve agent on survival rate of fatty tissue after cryopreservation.
 Methods: The liposuction was used on the abdomen of adult female. After centrifugation and purification, adipose was randomized into the following three groups, the trehalose group, the fetal bovine serum (FBS)+10%DMSO group and the physiological saline group. The specimens were cryopreserved at -196 ℃ for 3 months and then the HE staining, glucose transfer method and CK method were used to detect the cell survival rate in each group...
May 28, 2017: Zhong Nan da Xue Xue Bao. Yi Xue Ban, Journal of Central South University. Medical Sciences
https://www.readbyqxmd.com/read/28625922/macrophage-infiltration-regulates-the-adipose-ecm-reconstruction-and-the-fibrosis-process-after-fat-grafting
#11
Junrong Cai, Kaiyang Liu, Guanghui Li, Feng Lu
BACKGROUND: Fat grafting experiences a regeneration process from free lipoaspirate to intact adipose tissue. The adipose extracellular matrix (ECM) provides the structure and biochemical support for the surrounding cells, and is regulated by inflammatory cells, like macrophages. It is hypothesized that transferred fat undergoes ECM remodeling after fat grafting and the process can be regulated by macrophage infiltration. METHODS: Lipoaspirate was injected subcutaneously into the back of nude mice...
June 15, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28624714/patient-reported-outcomes-of-autologous-fat-grafting-after-breast-cancer-surgery
#12
A W W Brown, M Kabir, K A Sherman, F Meybodi, J R French, E B Elder
INTRODUCTION: Autologous fat grafting (AFG) can be used as an adjunct in breast cancer surgery to improve contour defects. Few previous studies have assessed patient reported outcomes (PROs) for AFG. This study analysed AFG use and assessed PROs in terms of physical and psychosocial well-being. MATERIALS AND METHODS: All patients undergoing AFG were identified from a prospective database and asked to complete the validated BREAST-Q questionnaire and a tool to assess patient-perceived change after AFG (5-point Likert-type scale)...
June 15, 2017: Breast: Official Journal of the European Society of Mastology
https://www.readbyqxmd.com/read/28622065/leptin-gene-transfer-improves-symptoms-of-type-2-diabetic-mice-by-regulating-leptin-signaling-pathway-and-insulin-resistance-of-peripheral-tissues
#13
Lan Xiang, Jing Li, Qian Wang, Ruiqi Tang, Jianhua Qi
The leptin gene was transferred into the liver of streptozocin- and high fat diet-induced type 2 diabetic (T2D) mice by hydrodynamic-based gene delivery. The food intake, water consumption, glucose concentration, and triglyceride, total cholesterol levels of T2D mice were significantly decreased. Meanwhile, plasma leptin was remarkably increased after gene transfer for 2, 3, 5, and 7 days, while plasma adiponectin was also significantly increased at day 2. To understand the mechanism of action of leptin on T2D mice, gene expressions related to glycometabolism and energy metabolism in the liver, epididymal adipose tissue, hypothalamus, and muscle were measured...
June 16, 2017: Human Gene Therapy
https://www.readbyqxmd.com/read/28610551/passive-cooling-of-cutaneous-and-subcutaneous-tissues-using-phase-changing-materials-feasibility-study-using-a-numerical-model
#14
Ricardo Romero-Méndez, Francisco G Pérez-Gutiérrez, Joseph J Musacchia, Walfre Franco
In many dermatological applications, lowering the temperature of skin and maintaining specific temperatures for extended periods of time are fundamental requirements for treatment; for example, in targeting adipose tissue and managing cutaneous pain. In this work we investigate the feasibility of using phase changing materials (PCMs) as an alternative passive, open-loop, heat extraction method for cooling cutaneous and subcutaneous tissues. We used a finite difference parametric approach to model the spatial and temporal progression of the heat transferred from the skin to a PCM in contact with the skin surface...
June 13, 2017: International Journal of Hyperthermia
https://www.readbyqxmd.com/read/28605392/transfer-of-mammary-gland-forming-ability-between-mammary-basal-epithelial-cells-and-mammary-luminal-cells-via-extracellular-vesicles-exosomes
#15
Meng-Chieh Lin, Shih-Yin Chen, Pei-Lin He, Wen-Ting Luo, Hua-Jung Li
Cells can communicate via exosomes, ~100-nm extracellular vesicles (EVs) that contain proteins, lipids, and nucleic acids. Non-adherent/mesenchymal mammary epithelial cell (NAMEC)-derived extracellular vesicles can be isolated from NAMEC medium via differential ultracentrifugation. Based on their density, EVs can be purified via ultracentrifugation at 110,000 x g. The EV preparation from ultracentrifugation can be further separated using a continuous density gradient to prevent contamination with soluble proteins...
June 3, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28599842/composite-breast-reconstruction-implant-based-breast-reconstruction-with%C3%A2-adjunctive-lipofilling
#16
C E Sommeling, K Van Landuyt, H Depypere, R Van den Broecke, S Monstrey, P N Blondeel, W A Morrison, F B Stillaert
INTRODUCTION: Options for breast reconstructions enclose autologous tissue transfers or implants. Fat grafting is gaining more interest in this specific field of breast surgery. This study concentrates on the technique and aesthetic results of breast reconstruction with fat grafts combined with implants, in women who have undergone total mastectomy. METHODS: Breast reconstructions (n = 23) was performed using a protocol of intratissular expansion with serial deflation-lipofilling...
May 22, 2017: Journal of Plastic, Reconstructive & Aesthetic Surgery: JPRAS
https://www.readbyqxmd.com/read/28597066/effects-of-fat-grafting-containing-stem-cells-in-microstomia-and-microcheilia-derived-from-systemic-sclerosis
#17
Oskar Blezien, Francesco D'Andrea, Giovanni Francesco Nicoletti, Giuseppe Andrea Ferraro
BACKGROUND: Systemic sclerosis (SSc) is an autoimmune disease with multisystem involvement, dominated by a general fibrosis. The early stage of the disease is associated with progressive damage to microcirculation, particularly in the respiratory tract, the gastrointestinal tract and skin. The face assumes a typical appearance characterized by microstomia (reduction of mouth opening) and microcheilia (thinning of the lips). These conditions cause a considerable reduction in performance status of patients...
June 8, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28578567/quantitative-profiling-of-endogenous-fat-soluble-vitamins-and-carotenoids-in-human-plasma-using-an-improved-uhpsfc-esi-ms-interface
#18
Filomena Petruzziello, Alexandre Grand-Guillaume Perrenoud, Anita Thorimbert, Michael Fogwill, Serge Rezzi
Analytical solutions enabling the quantification of circulating levels of liposoluble micronutrients such as vitamins and carotenoids are currently limited to either single or a reduced panel of analytes. The requirement to use multiple approaches hampers the investigation of the biological variability on a large number of samples in a time and cost efficient manner. With the goal to develop high-throughput and robust quantitative methods for the profiling of micronutrients in human plasma, we introduce a novel, validated workflow for the determination of 14 fat-soluble vitamins and carotenoids in a single run...
June 27, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28566529/unconditional-seasonal-cash-transfer-increases-intake-of-high-nutritional-value-foods-in-young-burkinabe-children-results-of-24-hour-dietary-recall-surveys-within-the-mam-out-randomized-controlled-trial
#19
Audrey Tonguet-Papucci, Freddy Houngbe, Lieven Huybregts, Myriam Ait-Aissa, Chiara Altare, Patrick Kolsteren, Jean-François Huneau
Background: Cash transfer programs have the potential to improve dietary intake by improving accessibility to food. However, quantitative data on the impact of cash transfer programs on children's energy and nutrient intakes are lacking.Objective: The aim of this study was to evaluate the effect of seasonal unconditional cash transfers on children's energy, micro- and macronutrient, and food group intakes during the lean season in Burkina Faso.Methods: Within the framework of the MAM'Out (Moderate Acute Malnutrition Out) cluster-randomized controlled trial, two 24-h dietary recall surveys were conducted in July and August 2014...
May 31, 2017: Journal of Nutrition
https://www.readbyqxmd.com/read/28566523/gastric-and-postgastric-processing-of-13-c-markers-renders-the-13-c-breath-test-an-inappropriate-measurement-method-for-the-gastric-emptying-of-lipid-emulsions-in-healthy-adults
#20
Helen L Parker, Dian Liu, Jelena Curcic, Marc-Olivier Ebert, Werner Schwizer, Michael Fried, Andreas Steingoetter
Background: Breath tests (BTs) present an alternative gastric-emptying (GE) measure. However, their efficacy in the measurement of the GE rate of lipid emulsions (LEs) is unknown.Objective: The objective of this work was to investigate the validity of (13)C BTs as a measure of fat GE rate in LEs.Methods: The lipophilic (13)C octanoate (OCC) BT marker was investigated for fat GE with the hydrophilic (13)C sodium acetate (ACC) and the triglyceride (13)C trioctanoin (TCC) markers as comparators. Data from 2 randomized studies were combined [50 healthy participants; 25 men, mean ± SD age: 23 ± 2...
May 31, 2017: Journal of Nutrition
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