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https://www.readbyqxmd.com/read/28822209/primary-culture-of-rat-aortic-vascular-smooth-muscle-cells-a-new-method
#1
Jufang Chi, Liping Meng, Sunlei Pan, Hui Lin, Xiaoya Zhai, Longbin Liu, Changzuan Zhou, Chengjian Jiang, Hangyuan Guo
BACKGROUND Developing a simple and efficient method of obtaining primary cultured VSMCs is necessary for basic cardiovascular research. MATERIAL AND METHODS The procedure of our new method mainly includes 6 steps: isolation of the aortic artery, removal of the fat tissue around the artery, separation of the media, cutting the media into small tissue blocks, transferring the tissue blocks to cell culture plates, and incubation until the cells reach confluence. The cells were identified as VSMCs by morphology and immunofluorescence...
August 19, 2017: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/28820834/super-thin-scip-flap-for-reconstruction-of-subungual-melanoma-aesthetic-functional-surgery
#2
Kyeong-Tae Lee, Bo Young Park, Eun-Ji Kim, Jung-Han Kim, Kee-Taek Jang, Sang-Hee Choi, Dong-Youn Lee, Goo-Hyun Mun
BACKGROUND: This study aimed to use a super-thin, free superficial circumflex iliac artery perforator (SCIP) flap in the functional surgery for treating subungual melanoma and to evaluate its outcomes. METHODS: Forty-one patients with primary subungual melanoma of ≤ 2-mm thickness who were treated with functional surgery were prospectively enrolled. Following oncologic resection, a thin SCIP flap was harvested along the trans-superficial fat layer and transferred to the defect with further thinning by primary defatting...
August 8, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28812002/image-guided-thermal-ablation-with-mr-based-thermometry
#3
REVIEW
Mingming Zhu, Ziqi Sun, Chin K Ng
Thermal ablation techniques such as radiofrequency, microwave, high intensity focused ultrasound (HIFU) and laser have been used as minimally invasive strategies for the treatment of variety of cancers. MR thermometry methods are readily available for monitoring thermal distribution and deposition in real time, leading to decrease of incidents of normal tissue damage around targeted lesion. HIFU and laser-induced thermal therapy (LITT) are the two widely accepted tumor ablation techniques because of their compatibility with MR systems...
June 2017: Quantitative Imaging in Medicine and Surgery
https://www.readbyqxmd.com/read/28811651/inkt-cells-prevent-obesity-induced-hepatic-steatosis-in-mice-in-a-c-c-chemokine-receptor-7-dependent-manner
#4
H M Kim, R Lee, E S Lee, M H Kwon, J H Huh, B-E Kwon, E-K Park, S-Y Chang, M-N Kweon, P-H Kim, H-J Ko, C H Chung
Non-alcoholic fatty liver disease and non-alcoholic steatohepatitis are characterized by an increase in hepatic triglyceride content with infiltration of immune cells, which can cause steatohepatitis and hepatic insulin resistance. C-C chemokine receptor 7 (CCR7) is primarily expressed in immune cells, and CCR7 deficiency leads to the development of multi-organ autoimmunity, chronic renal disease, and autoimmune diabetes. Here, we investigated the effect of CCR7 on hepatic steatosis in a mouse model and its underlying mechanism...
August 16, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/28799645/influence-of-a-three-stage-hybrid-ultrasound-osmotic-frying-process-on-production-of-low-fat-fried-potato-strips
#5
Jalal Dehghannya, Lida Abedpour
BACKGROUND: Fried potato is one of the most consumed products in the world. Due to consumers' growing tendency to use healthy and low-fat foods, reducing oil content in fried foods has become a necessity. Several studies have shown that higher initial water content results in increased oil uptake during frying. Therefore, pretreatments that reduce water content of the product could lower oil uptake in the final product. The aim of this study was to evaluate the influence of a three stage hybrid ultrasound-osmotic-frying process on production of low-fat fried potato strips...
August 11, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28795956/physical-signals-may-affect-mesenchymal-stem-cell-differentiation-via-epigenetic-controls
#6
Janet Rubin, Maya Styner, Gunes Uzer
Marrow mesenchymal stem cells supply bone osteoblasts and adipocytes. Exercise effects to increase bone and decrease fat involve transfer of signals from the cytoplasm into the nucleus to regulate gene expression. We propose that exercise control of stem cell fate relies on structural connections that terminate in the nucleus and involve intranuclear actin structures that regulate epigenetic gene expression.
August 8, 2017: Exercise and Sport Sciences Reviews
https://www.readbyqxmd.com/read/28793320/microsomal-triglyceride-transfer-protein-contributes-to-lipid-droplet-maturation-in-adipocytes
#7
Larry L Swift, Joseph D Love, Carla M Harris, Benny H Chang, W Gray Jerome
Previous studies in our laboratory have established the presence of MTP in both white and brown adipose tissue in mice as well as in 3T3-L1 cells. Additional studies demonstrated an increase in MTP levels as 3T3-L1 cells differentiate into adipocytes concurrent with the movement of MTP from the juxtanuclear region of the cell to the surface of lipid droplets. This suggested a role for MTP in lipid droplet biogenesis and/or maturation. To probe the role of MTP in adipocytes, we used a Cre-Lox approach with aP2-Cre and Adipoq-Cre recombinase transgenic mice to knock down MTP expression in brown and white fat of mice...
2017: PloS One
https://www.readbyqxmd.com/read/28792966/maternal-exposure-to-an-environmentally-relevant-dose-of-triclocarban-results-in-perinatal-exposure-and-potential-alterations-in-offspring-development-in-the-mouse-model
#8
Heather A Enright, Miranda J S Falso, Michael A Malfatti, Victoria Lao, Edward A Kuhn, Nicholas Hum, Yilan Shi, Ana Paula Sales, Kurt W Haack, Kristen S Kulp, Bruce A Buchholz, Gabriela G Loots, Graham Bench, Kenneth W Turteltaub
Triclocarban (TCC) is among the top 10 most commonly detected wastewater contaminants in both concentration and frequency. Its presence in water, as well as its propensity to bioaccumulate, has raised numerous questions about potential endocrine and developmental effects. Here, we investigated whether exposure to an environmentally relevant concentration of TCC could result in transfer from mother to offspring in CD-1 mice during gestation and lactation using accelerator mass spectrometry (AMS). 14C-TCC (100 nM) was administered to dams through drinking water up to gestation day 18, or from birth to post-natal day 10...
2017: PloS One
https://www.readbyqxmd.com/read/28791455/fat-injection-a-systematic-review-of-injection-volumes-by-facial-subunit
#9
REVIEW
Shirley Shue, David E Kurlander, Bahman Guyuron
BACKGROUND: Fat grafting to the aging face has become an integral component of esthetic surgery. However, the amount of fat to inject to each area of the face is not standardized and has been based mainly on the surgeon's experience. The purpose of this study was to perform a systematic review of injected fat volume to different facial zones. METHODS: A systematic review of the literature was performed through a MEDLINE search using keywords "facial," "fat grafting," "lipofilling," "Coleman technique," "autologous fat transfer," and "structural fat grafting...
August 8, 2017: Aesthetic Plastic Surgery
https://www.readbyqxmd.com/read/28789975/peripheral-neuropeptide-y-differentially-influences-adipogenesis-and-lipolysis-in-chicks-from-lines-selected-for-low-or-high-body-weight
#10
Lingbin Liu, Guoqing Wang, Yang Xiao, Steven L Shipp, Paul B Siegel, Mark A Cline, Elizabeth R Gilbert
Neuropeptide Y (NPY) stimulates appetite and promotes lipid deposition. We demonstrated a differential sensitivity in the food intake response to central NPY in chicks from lines selected for low (LWS) or high (HWS) body weight, but have not reported whether such differences exist in the periphery. At 5days, LWS and HWS chicks were intraperitoneally injected with 0 (vehicle), 60, or 120μg/kg BW NPY and subcutaneous adipose tissue and plasma were collected at 1, 3, 6, 12, and 24h (n=12). NPY injection increased glycerol-3-phosphate dehydrogenase (G3PDH) activity at 1 and 3h and reduced plasma NEFAs at 1 and 12h...
August 5, 2017: Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology
https://www.readbyqxmd.com/read/28783406/autologous-fat-transfer-for-breast-surgery
#11
Ketan Agarwal, Meera Mistry
Cosmetic and reconstructive surgeries always have a primary aim of the best outcome for the patient, with a big emphasis on the aesthetics. Lipomodelling is one such technique, originally described over a century ago but still used to this day, to provide a patient with the highest quality results. This is particularly true for lipomodelling of the breast, whether it be purely cosmetic, or for a post-mastectomy reconstruction for a breast cancer patient. Lipomodelling provides an effective way of restoring and even improving the look and aesthetics of the breast and of helping achieve the patient's expectations...
August 2, 2017: British Journal of Hospital Medicine
https://www.readbyqxmd.com/read/28774580/advancing-imaging-technologies-for-patients-with-spinal-pain-with-a-focus-on-whiplash-injury
#12
James M Elliott, Mark J Hancock, Rebecca J Crawford, Andrew C Smith, David M Walton
BACKGROUND CONTEXT: Radiological observations of soft-tissue changes that may relate to clinical symptoms in patients with traumatic and non-traumatic spinal disorders are highly controversial. Studies are often of poor quality and findings inconsistent. A plethora of evidence suggests some pathoanatomical findings from traditional imaging applications are common in asymptomatic participants across the life span, which further questions the diagnostic, prognostic, and theranostic value of traditional imaging...
July 31, 2017: Spine Journal: Official Journal of the North American Spine Society
https://www.readbyqxmd.com/read/28769122/modulation-of-gr1-low-monocytes-subset-impacts-insulin-sensitivity-and-weight-gain-upon-high-fat-diet-in-female-mice
#13
S Béliard, W Le Goff, F Saint-Charles, L Poupel, V Deaswerte, L Boucharechas, T Huby, P Lesnik
BACKGROUND/OBJECTIVES: Blood monocytes are expanded during obesity. However, the differential contribution of monocytes subsets in obesity-related metabolic disorders remains unknown. The aim of the study was to define the role of the Gr1(low) monocytes subset upon high-fat-diet (HFD). METHODS: We used transgenic female mouse models allowing the modulation of circulating Gr1(low) monocytes number (decreased number in CX3CR1(-/-) mice, increased number in CD11c-hBcl2 mice) and studied obesity upon HFD...
August 3, 2017: International Journal of Obesity: Journal of the International Association for the Study of Obesity
https://www.readbyqxmd.com/read/28749069/fatal-fat-embolism-after-penis-enlargement-by-autologous-fat-transfer-a-case-report-and-review-of-the-literature
#14
Brita Zilg, Petra Råsten-Almqvist
Fat embolism is an incidental finding in cases of long bone fractures or other trauma, but it is also associated with liposuction and autologous fat transfer, a procedure where fat from liposuction is injected back into the same patient's face, breast, buttocks or penis. We here present a case of sudden death by fat embolism in a healthy young male, caused by a simple penis enlargement procedure, in which fat was injected into the penis shaft. We suggest that the risk of fat embolization might be higher when pretraumatized tissue is subjected to fat injection, like in this case, where a penis elongation was performed before the fat injection...
July 27, 2017: Journal of Forensic Sciences
https://www.readbyqxmd.com/read/28746280/discussion-autologous-fat-transfer-for-thumb-carpometacarpal-joint-osteoarthritis-a-prospective-study
#15
COMMENT
Roger K Khouri, Roger K Khouri
No abstract text is available yet for this article.
August 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28746237/abdominoplasty-with-lymphatic-microsurgery-for-patients-with-secondary-lower-extremity-lymphedema
#16
Shinsuke Akita, Yoshihisa Yamaji, Hideki Tokumoto, Naoki Adachi, Yoshitaro Sasahara, Yoshitaka Kubota, Motone Kuriyama, Nobuyuki Mitsukawa
BACKGROUND: Patients with secondary lower extremity lymphedema (LEL) often develop suprapubic lymphedema. We developed a novel surgical method of shaping the lower abdomen and debulking suprapubic lymphedema with simultaneous reconstruction of lymphatic flow in case of LEL. METHODS: A skin incision in a fleur-de-lis pattern was performed for horizontal and vertical abdominal skin and fat resection. A caudal-based isosceles triangular flap was created on the central suprapubic region to reduce wound tension at the intersection of the horizontal and vertical incisions...
July 12, 2017: Plastic and Reconstructive Surgery
https://www.readbyqxmd.com/read/28739194/perforator-based-rectus-free-tissue-transfer-for-head-and-neck-reconstruction-new-reconstructive-advantages-from-an-old-friend
#17
Stephen Y Kang, Matthew E Spector, Douglas B Chepeha
OBJECTIVES: To demonstrate three reconstructive advantages of the perforator based rectus free tissue transfer: long pedicle, customizable adipose tissue, and volume reconstruction without muscle atrophy within a contained space. MATERIALS AND METHODS: Thirty patients with defects of the head and neck were reconstructed with the perforator based rectus free tissue transfer. RESULTS: Transplant success was 93%. Mean pedicle length was 13.4cm...
July 21, 2017: Oral Oncology
https://www.readbyqxmd.com/read/28717381/patterns-and-dynamics-of-neutral-lipid-fatty-acids-in-ants-implications-for-ecological-studies
#18
Félix B Rosumek, Adrian Brückner, Nico Blüthgen, Florian Menzel, Michael Heethoff
BACKGROUND: Trophic interactions are a fundamental aspect of ecosystem functioning, but often difficult to observe directly. Several indirect techniques, such as fatty acid analysis, were developed to assess these interactions. Fatty acid profiles may indicate dietary differences, while individual fatty acids can be used as biomarkers. Ants are among the most important terrestrial animal groups, but little is known about their lipid metabolism, and no study so far used fatty acids to study their trophic ecology...
2017: Frontiers in Zoology
https://www.readbyqxmd.com/read/28694154/short-photoperiod-reverses-obesity-in-siberian-hamsters-via-sympathetically-induced-lipolysis-and-browning-in-adipose-tissue
#19
REVIEW
Vitaly Ryu, Eleen Zarebidaki, H Elliott Albers, Bingzhong Xue, Timothy J Bartness
Changes in photoperiod length are transduced into neuroendocrine signals by melatonin (MEL) secreted by the pineal gland triggering seasonally adaptive responses in many animal species. Siberian hamsters, transferred from a long-day 'summer-like' photoperiod (LD) to a short-day 'winter-like' photoperiod (SD), exhibit a naturally-occurring reversal in obesity. Photoperiod-induced changes in adiposity are mediated by the duration of MEL secretion and can be mimicked by exogenously administered MEL into animals housed in LD...
July 7, 2017: Physiology & Behavior
https://www.readbyqxmd.com/read/28691463/use-of-the-orbital-fat-pad-in-eyelid-reconstruction
#20
A Baj, O D Orto, M Romano, G A Beltramini, F J Silvestre, A B Giannì
Eyelid reconstruction, though challenging, can produce a functional, esthetically pleasing lid. This should preferably be accomplished with a one-stage procedure, using robust, well-vascularized tissues similar to those being replaced, with minimal associated donor morbidity. According to the experience with Bichat pedicled flap in oral reconstruction, the authors propose the use of pedicle infraorbital fat pad for repair of conjunctival defect. Seven patients underwent eyelid reconstruction of full-thickness oncological or post-traumatic defects...
April 2017: Journal of Biological Regulators and Homeostatic Agents
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