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Agnieszka Uryga, Michał M Placek, Paweł Wachel, Tomasz Szczepański, Marek Czosnyka, Magdalena Kasprowicz
We aim to investigate whether phase shift between respiratory oscillations in cerebral blood flow velocity (CBFV) and arterial blood pressure (ABP) is associated with changes in cerebral autoregulation (CA) or reflects the mechano-elastic properties of the cerebrovascular bed. The relationships between respiratory phase shift and slow wave phase shift versus cerebrovascular time constant (the product of cerebrovascular resistance and compliance) and the index of CA (Mx) were analyzed during breathing at 6, 10, and 15 breaths min(-1) in 39 volunteers...
January 18, 2017: Physiological Measurement
Fung-Wei Chang, Hueng-Chuen Fan, Jui-Ming Liu, Tai-Ping Fan, Jin Jing, Chia-Ling Yang, Ren-Jun Hsu
BACKGROUND: Multidrug resistance is a major obstacle in the successful therapy of breast cancer. Studies have proved that this kind of drug resistance happens in both human cancers and cultured cancer cell lines. Understanding the molecular mechanisms of drug resistance is important for the reasonable design and use of new treatment strategies to effectively confront cancers. RESULTS: In our study, ATP-binding cassette sub-family G member 2 (ABCG2), adenosine triphosphate (ATP) synthase and cytochrome c oxidase subunit VIc (COX6C) were over-expressed more in the MCF-7/MX cell line than in the normal MCF7 cell line...
January 14, 2017: International Journal of Molecular Sciences
Jing Zhou, Shi-Qi Wang, Jian-Chao Wei, Xiao-Min Zhang, Zhi-Can Gao, Ke Liu, Zhi-Yong Ma, Pu-Yan Chen, Bin Zhou
Mx proteins are interferon (IFN)-induced dynamin-like GTPases that are present in all vertebrates and inhibit the replication of myriad viruses. However, the role Mx proteins play in IFN-mediated suppression of Japanese encephalitis virus (JEV) infection is unknown. In this study, we set out to investigate the effects of Mx1 and Mx2 expression on the interferon-α (IFNα) restriction of JEV replication. To evaluate whether the inhibitory activity of IFNα on JEV is dependent on Mx1 or Mx2, we knocked down Mx1 or Mx2 with siRNA in IFNα-treated PK-15 cells and BHK-21 cells, then challenged them with JEV; the production of progeny virus was assessed by plaque assay, RT-qPCR, and Western blotting...
January 10, 2017: Viruses
Wei Ouyang, Yong-Shan Wang, Kai Meng, Qun-Xing Pan, Xiao-Li Wang, Xing-Xia Xia, Yu-Mei Zhu, Zhen-Wei Bi, Hai-Bin Zhang, Kai Luo
Infectious bursal disease (IBD) is characterized by the immune suppression of infected birds. The molecular mechanism by which IBD virus (IBDV) suppresses the host immune system remains to be elucidated. The tumor suppressor protein p53 can inhibit the replication of various viruses, but its effect on IBDV remains unknown. This study established an in vitro infection model based on DF-1 cells (chicken embryo fibroblast cell line) to investigate the antiviral effects of chicken p53 (chp53) on IBDV infection...
January 2017: Veterinary Microbiology
Michał Jaszuński, Andrej Antušek, Taye B Demissie, Stanislav Komorovsky, Michal Repisky, Kenneth Ruud
We report the Nuclear Magnetic Resonance (NMR) spin-spin coupling constants for diatomic alkali halides MX, where M = Li, Na, K, Rb, or Cs and X = F, Cl, Br, or I. The coupling constants are determined by supplementing the non-relativistic coupled-cluster singles-and-doubles (CCSD) values with relativistic corrections evaluated at the four-component density-functional theory (DFT) level. These corrections are calculated as the differences between relativistic and non-relativistic values determined using the PBE0 functional with 50% exact-exchange admixture...
December 28, 2016: Journal of Chemical Physics
C López-Vázquez, M C Alonso, C P Dopazo, I Bandín
The effect of IPNV-VHSV coinfection and superinfection on the mortality caused by both viruses in Senegalese sole has been analysed. No effect was observed after coinfection. However, a clear viral interference was recorded between a primary IPNV and a subsequent VHSV infection, which led to a survival increase in the infected sole of 50% points when compared with fish infected only with VHSV. The significantly higher Mx transcriptional values in the fish pre-exposed to IPNV (at least at first days after superinfection) and the increased daily mortality when low Mx transcriptional levels were recorded suggest that Mx may be involved in the protective effect against VHSV infection...
December 29, 2016: Journal of Fish Diseases
Sina Soleimani, Shahla Shahsavandi, Omid Maddadgar
Immunization with DNA vaccines as a novel alternative to conventional vaccination strategy requires adjuvant for improving vaccine efficacy. The conserved immunogenic HA2 subunit, which harbors neutralizing epitopes is a promising vaccine candidate against influenza viruses. In this study, for the first time we explore the idea of using host interferon inducible Mx protein to increase the immunogenicity of HA2 H9N2 influenza DNA vaccine. The potency and safety of the Mx adjuvanted-HA2 vaccine was evaluated in BALB/c mice by different prime-boost strategies...
December 24, 2016: Biologicals: Journal of the International Association of Biological Standardization
Ting-Ting Guo, Li-Li Li, Xing-Yuan Men, Zeng-Bin Lu, Hao Chen, Zhen-Ying Wang, Ting-Lin Sun, Yi Yu
Athetis dissimilis (Hampson) has emerged as a serious pest on corn in recent years in China. Understanding the population response of A. dissimilis to temperature will be beneficial for adopting control strategies for this pest. The impact of five constant temperatures (17, 21, 25, 29, and 33 °C) on the life table of A. dissimilis was studied using age-stage, two-sex life table method in the laboratory. The results showed that the developmental time of egg, larva, pupa, and adult decreased when temperature increased from 17 °C to 33 °C...
December 23, 2016: Journal of Economic Entomology
Jonathan C Brooks-Bartlett, Rebecca A Batters, Charles S Bury, Edward D Lowe, Helen Mary Ginn, Adam Round, Elspeth F Garman
Biological small-angle X-ray scattering (SAXS) is an increasingly popular technique used to obtain nanoscale structural information on macromolecules in solution. However, radiation damage to the samples limits the amount of useful data that can be collected from a single sample. In contrast to the extensive analytical resources available for macromolecular crystallography (MX), there are relatively few tools to quantitate radiation damage for SAXS, some of which require a significant level of manual characterization, with the potential of leading to conflicting results from different studies...
January 1, 2017: Journal of Synchrotron Radiation
Eva Crosas, Albert Castellvi, Isidro Crespo, Daniel Fulla, Fernando Gil-Ortiz, Gustavo Fuertes, Christina S Kamma-Lorger, Marc Malfois, Miguel A G Aranda, Jordi Juanhuix
Macromolecular crystallography (MX) and small-angle X-ray scattering (SAXS) studies on proteins at synchrotron light sources are commonly limited by the structural damage produced by the intense X-ray beam. Several effects, such as aggregation in protein solutions and global and site-specific damage in crystals, reduce the data quality or even introduce artefacts that can result in a biologically misguiding structure. One strategy to reduce these negative effects is the inclusion of an additive in the buffer solution to act as a free radical scavenger...
January 1, 2017: Journal of Synchrotron Radiation
Elena I Davydova, Anna S Lisovenko, Alexey Y Timoshkin
Complex beryllium amidoboranes Mx [Be(NH2 BH3 )x+2 ] (M = Li-Cs, x = 1,2) have been computationally studied at M06-2X/def2-TZVPPD//B3LYP/def2-TZVPPD level of theory. Compounds are predicted to be stable at room temperature but release H2 on heating. Agostic Be…HB bonds play an important role in stabilization of oligomeric beryllium imidoboranes. Polymeric imidoborane, hydrogen, and ammonia are expected as major thermal decomposition products of complex beryllium amidoboranes. Ammonia evolution is predicted to proceed at slightly higher temperatures than hydrogen evolution...
December 19, 2016: Journal of Computational Chemistry
Wojciech A Sławiński, Anja Olafsen Sjåstad, Helmer Fjellvåg
Layered double hydroxides (LDH) are a broad group of widely studied materials. The layered character of those materials and their high flexibility for accommodating different metals and anions make them technologically interesting. The general formula for the LDH compound is [M1-x(II)Mx(III)(OH)2][A(n-)]x/n·mH2O, where M(II) is a divalent metal cation which can be substituted by M(III) trivalent cation, and A(n-) is a charge compensating anion located between positively charged layers. In this paper we present a comprehensive study on possible structural disorder in LDH...
December 19, 2016: Inorganic Chemistry
Kyle M Stiers, Christopher B Lee, Jay C Nix, John J Tanner, Lesa J Beamer
This paper describes the introduction of synchrotron-based macromolecular crystallography (MX) into an undergraduate laboratory class. An introductory 2 week experimental module on MX, consisting of four laboratory sessions and two classroom lectures, was incorporated into a senior-level biochemistry class focused on a survey of biochemical techniques, including the experimental characterization of proteins. Students purified recombinant protein samples, set up crystallization plates and flash-cooled crystals for shipping to a synchrotron...
December 1, 2016: Journal of Applied Crystallography
Leo Hanke, Kevin E Knockenhauer, R Camille Brewer, Eline van Diest, Florian I Schmidt, Thomas U Schwartz, Hidde L Ploegh
: Alpaca-derived single-domain antibody fragments (VHHs) that target the influenza A virus nucleoprotein (NP) can protect cells from infection when expressed in the cytosol. We found that one such VHH, αNP-VHH1, exhibits antiviral activity similar to that of Mx proteins by blocking nuclear import of incoming viral ribonucleoproteins (vRNPs) and viral transcription and replication in the nucleus. We determined a 3.2-Å crystal structure of αNP-VHH1 in complex with influenza A virus NP...
December 13, 2016: MBio
Zhao-Hai Zeng, Chao-Chao Du, Shi-Rao Liu, Hui Li, Xuan-Xian Peng, Bo Peng
We have recently reported that the survival of tilapia, Oreochromis niloticus, during Edwardsiella tarda infection is tightly associated with their metabolome, where the survived O. niloticus has distinct metabolomic profile to dying O. niloticus. Glucose is the key metabolite to distinguish the survival- and dying-metabolome. More importantly, exogenous administration of glucose to the fish greatly enhances their survival for the infection, indicating the functional roles of glucose in metabolome repurposing, known as reprogramming metabolomics...
December 11, 2016: Fish & Shellfish Immunology
Wei Chen, Yayuan Liu, Yuzhang Li, Jie Sun, Yongcai Qiu, Chong Liu, Guangmin Zhou, Yi Cui
Transition metal dichalcogenides have been widely studied as active electrocatalysts for hydrogen evolution reactions. However, their properties as oxygen evolution reaction catalysts have not been fully explored. In this study, we systematically investigate a family of transition metal dichalcogenides (MX, M = Co, Ni, Fe; X = S, Se, Te) as candidates for water oxidation. It reveals that the transition metal dichalcogenides are easily oxidized in strong alkaline media via an in situ electrochemical oxidation process, producing nanoporous transition metal oxides toward much enhanced water oxidation activity due to their increased surface area and more exposed electroactive sites...
December 14, 2016: Nano Letters
Shuo Li, Weijiao Peng, Gaixiang Hao, Jiafang Li, Xuyun Geng, Jinsheng Sun
Dual-specificity phosphatase 6 (Dusp6) is a member of mitogen-activated protein kinase (MAPK) phosphatases that play crucial roles in regulating MAPK signaling and immune response. The immunological relevance of Dusp6 in fish, however, remains largely uncharacterized. In the present study, a full-length Japanese flounder dusp6 cDNA ortholog, termed PoDusp6, was identified and characterized from Paralichthys olivaceus. The deduced PoDusp6 protein is comprised of 383 amino acids with a conserved N-terminal regulatory rhodanese homology domain and a C-terminal catalytic domain...
December 8, 2016: Fish & Shellfish Immunology
Youyu Zhang, Lixing Huang, Yujie Zhao, Tianhui Hu
Musk xylene (MX) is a widely used synthetic nitro musk. Although the persistence and bioaccumulation of the synthetic musks are of concern since the nineteenth century, knowledge concerning the toxicity and environmental risks, especially the carcinogenicity is still limited. In the present study, the normal human hepatic cell line L02 was used to investigate the long-term carcinogenicity of MX. L02 cells were exposed to MX with different concentrations (10, 100, and 1000 μg/L) for 24 h, then with conventional culture...
February 2017: Chemosphere
Maolida Nihemaiti, Julien Le Roux, Christiane Hoppe-Jones, David A Reckhow, Jean-Philippe Croué
The potential formation of nitrogenous disinfection byproducts (N-DBPs) was investigated from the chloramination of nitrogenous and non-nitrogenous aromatic compounds. All molecules led to the formation of known N-DBPs (e.g., dichloroacetonitrile, dichloroacetamide) with various production yields. Resorcinol, a major precursor of chloroform, also formed di/trichloroacetonitrile, di/trichloroacetamide, and haloacetic acids, indicating that it is a precursor of both N-DBPs and carbonaceous DBPs (C-DBPs) upon chloramination...
January 3, 2017: Environmental Science & Technology
Anetta Lasek-Bal, Karina Bartoszek, Arkadiusz Steposz, Przemyslaw Puz, Wieslaw Bal, Zofia Kazibutowska
: Mitoxantrone (MX) is used in patients with primary and secondary progressive as well as relapsing-remitting type of multiple sclerosis (PPMS, SPMS, RRMS). The objective of our project was to evaluate the efficacy and safety of MX use in patients with PPMS and SPMS. METHODS: The retrospective study included 104 patients (mean age 54.2 ± 9.0), with PPMS (13.46%) and SPMS (86.54%) treated with MX. During single cycle of the MX therapy a dose of 12 mg/m(2) of body surface area was administered and next cycles every three months up to a total dose of 140 mg/m(2) were realized...
December 23, 2016: International Journal of Neuroscience
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