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https://www.readbyqxmd.com/read/29438933/assessment-of-cr-pollution-in-tributary-sediment-cores-in-the-three-gorges-reservoir-combining-geochemical-baseline-and-in-situ-dgt
#1
Bo Gao, Li Gao, Dongyu Xu, Yang Zhou, Jin Lu
The mobility and transfer of trace metals in sediments are vital to understanding trace metals environmental behavior in water environment. However, as a predominant aquatic carcinogen, an effective method for assessing the release and deposition for Cr at the sediment-water interface (SWI) is still not clearly understood. Here we established a comprehensive methodology to evaluate the release risk of Cr at the SWI combining regional geochemical baseline (RGB) and diffusive gradients in thin films (DGT). Sediment cores and water samples were collected in the two tributaries and mainstream of the Three Gorges Reservoir, which is the world's largest man-made hydroelectric station...
February 10, 2018: Science of the Total Environment
https://www.readbyqxmd.com/read/29429806/a-live-attenuated-vibrio-anguillarum-vaccine-induces-efficient-immunoprotection-in-tiger-puffer-takifugu-rubripes
#2
Xiaohong Liu, Chenglong Jiao, Yue Ma, Qiyao Wang, Yuanxing Zhang
Tiger puffer (Takifugu rubripes) is becoming an economic promising aquaculture species in China. However, the development of Tiger puffer breeding industry is restricted by some serious aquatic disease such as vibriosis. An effective live attenuated vaccine MVAV6203 was developed in our previous studies by curing the virulence plasmid pEIB1 and unmarked inframe-deletion of the aroC gene from the virulent V. anguillarum. Here, we evaluated whether this live vaccine was suitable for Tiger puffer against disease caused by Vibrio genus...
February 8, 2018: Vaccine
https://www.readbyqxmd.com/read/29422897/fowl-adenovirus-serotype-4-sd0828-infections-causes-high-mortality-rate-and-cytokine-levels-in-specific-pathogen-free-chickens-compared-to-ducks
#3
Rong Li, Gen Li, Jing Lin, Shaojie Han, Xiaolan Hou, Hongyu Weng, Mengjiao Guo, Zhong Lu, Ning Li, Yingli Shang, Tongjie Chai, Liangmeng Wei
Hydropericardium syndrome and inclusion body hepatitis, together called hydropericardium-hepatitis syndrome, are acute infectious diseases found in chickens. These diseases are caused primarily by fowl adenovirus serotype 4 (FAdV-4) strains. In this study, we isolated a FAdV-4 strain (SD0828) from clinically diseased chickens and phylogenetically analyzed the L1 loops of the hexon protein sequences in 3-week-old specific pathogen-free chickens and ducks infected intramuscularly and orally, determining differences in the pathogenicity by observing clinical signs and gross and histological lesions...
2018: Frontiers in Immunology
https://www.readbyqxmd.com/read/29413814/interferon-induced-mx-protein-from-indian-snow-trout-schizothorax-richardsonii-gray-lacks-critical-functional-features-unlike-its-mammalian-homologues
#4
Ankur Saxena, Kiran Belwal, Ankita Chauhan, Amit Pande
Viral attack within host cells triggers the production of type I interferons and leads to the induction of interferon stimulated genes (ISGs). One of the ISG Mx, encodes type I interferon inducible GTPase that is responsible for the establishment of an anti-viral state within cells. Intriguingly, several isoforms of Mx have been reported in fish, but the structural analysis of fish Mx proteins remains unexplored. For the first time, we have identified and unraveled the molecular structure of Mx protein from Indian snow trout, Schizothorax richardsonii (Gray) a Coldwater fish that inhabits the water bodies in the sub-Himalayan region...
January 31, 2018: Computational Biology and Chemistry
https://www.readbyqxmd.com/read/29411244/strain-screening-from-traditional-fermented-soybean-foods-and-induction-of-nattokinase-production-in-bacillus-subtilis-mx-6
#5
Li-Li Man, Dian-Jun Xiang, Chun-Lan Zhang
The plasminogen-free fibrin plate assay method was used to isolate Bacillus subtilis MX-6, a strain with high production of nattokinase from Chinese douchi. The presence of aprN, a gene-encoding nattokinase, was verified with PCR method. The predicted amino acid sequence was aligned with homologous sequences, and a phylogenetic tree was constructed. Nattokinase was sublimated with ammonium sulfate, using a DEAE-Sepharose Fast Flow column, a CM-Sepharose Fast Flow column and a Sephadex G-75 gel filtration column...
February 6, 2018: Probiotics and Antimicrobial Proteins
https://www.readbyqxmd.com/read/29407578/a-rapid-and-transient-innate-immune-response-to-avian-influenza-infection-in-mallards
#6
Anu S Helin, Michelle Wille, Clara Atterby, Josef D Järhult, Jonas Waldenström, Joanne R Chapman
The vertebrate innate immune system provides hosts with a rapid, non-specific response to a wide range of invading pathogens. However, the speed and duration of innate responses will be influenced by the co-evolutionary dynamics of specific host-pathogen combinations. Here, we show that low pathogenic avian influenza virus (LPAI) subtype H1N1 elicits a strong but extremely transient innate immune response in its main wildlife reservoir, the mallard (Anas platyrhynchos). Using a series of experimental and methodological improvements over previous studies, we followed the expression of retinoic acid inducible gene 1 (RIG-I) and myxovirus resistance gene (Mx) in mallards semi-naturally infected with low pathogenic H1N1...
February 2, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29407574/pathogenicity-of-duck-hepatitis-a-virus-type-3-and-innate-immune-responses-of-the-ducklings-to-virulent-dhav-3
#7
Xuelian Zhang, Chong Cao, Zhehui Qu, Wenjing Zhang, Yue Liu, Haihui Qi, Chunxue Hao, Wenlong Zhang, Mingchun Gao, Junwei Wang, Bo Ma
Duck virus hepatitis caused by duck hepatitis A virus (DHAV) is an acute and contagious disease. To better understand the pathogenic mechanism of DHAV-3 in ducklings, an infection experiment was performed. Our results showed that typical symptoms were observed in the infected ducklings. DHAV-3 could infect many tissues, leading to pathological lesions, especially on the livers and spleen, and the host immune responses are activated in infection. Real-time quantitative PCR demonstrated that expression of many innate immune-related genes was mostly up-regulated in the livers and spleen, and antiviral innate immune response was established, but not sufficient to restrict the virus replication of lethal dose...
January 26, 2018: Molecular Immunology
https://www.readbyqxmd.com/read/29399125/identification-of-potentially-relevant-genes-for-myocardial-infarction-using-rna-sequencing-data-analysis
#8
Qiang Zhao, Ke Wu, Nannan Li, Zhengmei Li, Fenglin Jin
Myocardial infarction (MI) is a heart disease with high morbidity and mortality rates, thus it is critical to identify genes that serve roles during its pathogenesis. The objective of the present study was to identify potentially relevant genes during the progression of the disease. Blood samples from patients with MI and normal controls (n=3/group) were obtained, the RNA was extracted and cDNA libraries were established. RNA sequencing (RNA-seq) was performed on a HiSeq 2500 platform and fragments per kilobase of exon per million fragments mapped was utilized to calculate the gene expression value following preprocessing of the RNA-seq data...
February 2018: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29397783/estimating-pressure-reactivity-index-using-non-invasive-doppler-based-systolic-flow-index
#9
Frederick Adam Zeiler, Peter Smieleweski, Joseph Donnelly, Marek Czosnyka, David Menon, Ari Ercole
To derive models that estimate pressure reactivity index (PRx) using non-invasive transcranial Doppler (TCD) based systolic flow index (Sx_a) and mean flow index (Mx_a), both based on mean arterial pressure, in traumatic brain injury (TBI). Using a retrospective database of 347 TBI patients with ICP and TCD time series recordings we derived PRx, Sx_a and Mx_a. We first derived the autocorrelative structure of PRx based on: A. autoregressive integrative moving average (ARIMA) modelling in representative patients, and B...
February 3, 2018: Journal of Neurotrauma
https://www.readbyqxmd.com/read/29385554/effect-of-polygonum-persicaria-polygonales-polygonaceae-extracted-agglutinin-on-life-table-and-antioxidant-responses-in-helicoverpa-armigera-lepidoptera-noctuidae-larvae
#10
Vahid Rahimi, Jalil Hajizadeh, Arash Zibaee, Jalal Jalali Sendi
Plant lectins could reduce insect populations by imposing imbalances in biology and physiology. Here, an agglutinin was extracted from Polygonum persicaria L. (PPA; Polygonales: Polygonaceae) and its effects were investigated on life table parameters and antioxidant system of Helicoverpa armigera Hübner (Lepidoptera: Noctuidae). PPA significantly changed demographic parameters showing adverse effects on age-stage survival rate (Sxj), age-specific survival rate (lx), age-specific fecundity rate (mx), age stage specific fecundity (fxj), and life expectancy (exj)...
January 27, 2018: Journal of Economic Entomology
https://www.readbyqxmd.com/read/29383848/higher-ca2-sensitivity-of-arterial-contraction-in-1-week-old-rats-is-due-to-a-greater-rho-kinase-activity
#11
S V Mochalov, N V Tarasova, T V Kudryashova, D K Gaynullina, V U Kalenchuk, A S Borovik, A V Vorotnikov, O S Tarasova, R Schubert
AIM: During early postnatal development, arterial contraction depends less on Ca2+ -signalling pathways but more on changes in Ca2+ -sensitivity compared to adult animals. Whether this difference is related to Rho-kinase, one of the major players affecting Ca2+ -sensitivity, is unknown for intact vessels. Thus, we tested the hypothesis that Rho-kinase critically contributes to the higher Ca2+ -sensitivity of contraction in intact arteries of 1-week old rats. METHODS: We studied 1-week-old, 4-5-week-old and 10-12-week-old rats performing isometric myography, Ca2+ -fluorimetry and Western blotting using intact saphenous arteries and arterial pressure measurements under urethane anesthesia...
January 31, 2018: Acta Physiologica
https://www.readbyqxmd.com/read/29377042/a-superhydrophobic-cone-to-facilitate-the-xenomonitoring-of-filarial-parasites-malaria-and-trypanosomes-using-mosquito-excreta-feces
#12
Darren A N Cook, Nils Pilotte, Corrado Minetti, Steven A Williams, Lisa J Reimer
Background: Molecular xenomonitoring (MX), the testing of insect vectors for the presence of human pathogens, has the potential to provide a non-invasive and cost-effective method for monitoring the prevalence of disease within a community. Current MX methods require the capture and processing of large numbers of mosquitoes, particularly in areas of low endemicity, increasing the time, cost and labour required. Screening the excreta/feces (E/F) released from mosquitoes, rather than whole carcasses, improves the throughput by removing the need to discriminate vector species since non-vectors release ingested pathogens in E/F...
November 6, 2017: Gates Open Res
https://www.readbyqxmd.com/read/29368006/the-adenosine-receptor-antagonist-7-methylxanthine-alters-emmetropizing-responses-in-infant-macaques
#13
Li-Fang Hung, Baskar Arumugam, Lisa Ostrin, Nimesh Patel, Klaus Trier, Monica Jong, Earl L Smith Iii
Purpose: Previous studies suggest that the adenosine receptor antagonist, 7-methylxanthine (7-MX), retards myopia progression. Our aim was to determine whether 7-MX alters the compensating refractive changes produced by defocus in rhesus monkeys. Methods: Starting at age 3 weeks, monkeys were reared with -3 diopter (D; n = 10; 7-MX -3D/pl) or +3D (n = 6; 7-MX +3D/pl) spectacles over their treated eyes and zero-powered lenses over their fellow eyes. In addition, they were given 100 mg/kg of 7-MX orally twice daily throughout the lens-rearing period (age 147 ± 4 days)...
January 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29364850/protective-immunity-induced-by-dna-vaccination-against-ranavirus-infection-in-chinese-giant-salamander-andrias-davidianus
#14
Zhong-Yuan Chen, Tao Li, Xiao-Chan Gao, Chen-Fei Wang, Qi-Ya Zhang
Andrias davidianus ranavirus (ADRV) is an emerging viral pathogen that causes severe systemic hemorrhagic disease in Chinese giant salamanders. There is an urgent need for developing an effective vaccine against this fatal disease. In this study, DNA vaccines containing the ADRV 2L gene (pcDNA-2L) and the 58L gene (pcDNA-58L) were respectively constructed, and their immune protective effects were evaluated in Chinese giant salamanders. In vitro and in vivo expression of the vaccine plasmids were confirmed in transfected cells and muscle tissues of vaccinated Chinese giant salamanders by using immunoblot analysis or RT-PCR...
January 24, 2018: Viruses
https://www.readbyqxmd.com/read/29360049/analysis-of-the-elastic-bending-characteristics-of-cementless-short-hip-stems-considering-the-valgus-alignment-of-the-prosthetic-stem
#15
Alexander Jahnke, Carlos A Fonseca Ulloa, Jörn Bengt Seeger, Markus Rickert, Gerhard Walter Jahnke, Gafar Adam Ahmed, Bernd Alexander Ishaque
BACKGROUND: The resultant hip force causes a varus torque which must be compensated by a shear force couple depending on the stem alignment of the prosthesis. Since the prosthesis is substantially less flexible than the bone, the interior of the femur is stiffened over the entire prosthesis length. The present study thus aims at analyzing short-stem prostheses for its elastic bending characteristics, considering inappropriate valgus alignment of the prosthetic stem. METHODS: Five short stem prostheses were implanted each in synthetic femora in a standardized manner - in neutral and valgus stem alignments...
January 19, 2018: Clinical Biomechanics
https://www.readbyqxmd.com/read/29343573/porcine-mx1-protein-inhibits-classical-swine-fever-virus-replication-by-targeting-nonstructural-protein-ns5b
#16
Jing Zhou, Jing Chen, Xiao-Min Zhang, Zhi-Can Gao, Chun-Chun Liu, Yun-Na Zhang, Jin-Xiu Hou, Zhao-Yao Li, Lin Kan, Wen-Liang Li, Bin Zhou
Mx proteins are interferon-induced GTPases that have broad antiviral activity against a wide range of RNA and DNA viruses; they belong to the dynamin superfamily of large GTPases. In this study, we confirmed the anti-CSFV activity of porcine Mx1 in vitro and showed that porcine Mx2 (poMx2), human MxA (huMxA), and mouse Mx1 (mmMx1) also have anti-CSFV activity in vitro. siRNA experiments revealed that depletion of endogenous poMx1 or poMx2 enhanced CSFV replication, suggesting that porcine Mx proteins are responsible for the antiviral activity of interferon-α (IFNα) against CSFV infection...
January 17, 2018: Journal of Virology
https://www.readbyqxmd.com/read/29343099/modelling-the-water-plant-cuticular-polymer-matrix-membrane-partitioning-of-diverse-chemicals-in-multiple-plant-species-using-the-support-vector-machine-based-qsar-approach
#17
S Gupta, S Mallick
In this study, a support vector machine (SVM) based multi-species QSAR (quantitative structure-activity relationship) model was developed for predicting the water-plant cuticular polymer matrix membrane (MX) partition coefficient, KMXw of diverse chemicals using two simple molecular descriptors derived from the chemical structures and following the OECD guidelines. Accordingly, the Lycopersicon esculentum Mill. data were used to construct the QSAR model that was externally validated using three other plant species data...
January 18, 2018: SAR and QSAR in Environmental Research
https://www.readbyqxmd.com/read/29341929/the-infection-of-red-seabream-iridovirus-in-mandarin-fish-siniperca-chuatsi-and-the-host-immune-related-gene-expression-profiles
#18
Xiaodan Liu, Nan Chen, Xiaojian Gao, Yingying Zhang, Xixi Li, Yue Zhang, Xuwen Bing, Hezhong Huang, Xiaojun Zhang
Red Sea bream iridovirus (RSIV) was initially isolated from marine fish, which belongs to Megalocytivirus, Iridoviridae. It can cause great economic losses in fish culture with high morbidity and mortality. In the present study, the pathogenicity and immune response associated with a RSIV genotype megalocytivirus infection were determined in mandarin fish (Siniperca chuatsi). Fish challenged showed typical clinical signs of iridovirus infection, including acute haemorrhages and enlarged visceral organs. Histopathological analysis revealed that extensive necrosis, vacuolization and inflammation were presented in the stomach, spleen, kidney and liver of the diseased fish...
January 13, 2018: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29337573/cryoprotectants-severely-exacerbate-x-ray-induced-photoreduction
#19
Kurt H Nienaber, M Jake Pushie, Julien J H Cotelesage, Ingrid J Pickering, Graham N George
Approximately 11% of enzymes contain a transition metal ion that is essential for catalytic function. Such metalloenzymes catalyze much of the most chemically challenging and biologically essential chemistry carried out by life. X-ray based methods, predominantly macromolecular crystallography (MX) and also X-ray absorption spectroscopy (XAS), have proved essential for determining structures of transition metal ion-containing active sites in order to deduce enzyme catalytic mechanisms. However, X-ray irradiation can induce change in both oxidation state and structure of the metal, which is problematic in structure determination...
January 16, 2018: Journal of Physical Chemistry Letters
https://www.readbyqxmd.com/read/29325821/dna-vaccine-encoding-myristoylated-membrane-protein-mmp-of-rock-bream-iridovirus-rbiv-induces-protective-immunity-in-rock-bream-oplegnathus-fasciatus
#20
Myung-Hwa Jung, Chamilani Nikapitiya, Sung-Ju Jung
Rock bream iridovirus (RBIV) causes severe mass mortalities in rock bream (Oplegnathus fasciatus) in Korea. In this study, we investigated the potential of viral membrane protein to induce antiviral status protecting rock bream against RBIV infection. We found that fish administered with ORF008L (myristoylated membrane protein, MMP) vaccine exhibited significantly higher levels of survival compared to ORF007L (major capsid protein, MCP). Moreover, ORF008L-based DNA vaccinated fish showed significant protection at 4 and 8 weeks post vaccination (wpv) than non-vaccinated fish after infected with RBIV (6...
January 8, 2018: Vaccine
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