Raman M Das, Nick J Van Hateren, Gareth R Howell, Elizabeth R Farrell, Fiona K Bangs, Victoria C Porteous, Elizabeth M Manning, Michael J McGrew, Kyoji Ohyama, Melanie A Sacco, Pam A Halley, Helen M Sang, Kate G Storey, Marysia Placzek, Cheryll Tickle, Venugopal K Nair, Stuart A Wilson
RNA interference (RNAi) provides an effective method to silence gene expression and investigate gene function. However, RNAi tools for the chicken embryo have largely been adapted from vectors designed for mammalian cells. Here we present plasmid and retroviral RNAi vectors specifically designed for optimal gene silencing in chicken cells. The vectors use a chicken U6 promoter to express RNAs modelled on microRNA30, which are embedded within chicken microRNA operon sequences to ensure optimal Drosha and Dicer processing of transcripts...
June 15, 2006: Developmental Biology