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Jie Liu, Liping Yang, Lei Gong, Yuanyuan Gu, Yilin Wang, Chengming Sun, Yuanyuan Hou
The occurrence of infertility in diabetic patients is attributed to oxidative damage of peroxidized products. High glucose-induced mitochondrial oxidative stress and glycolytic enzyme inactivation is considered to be an important mediator for sperm dysfunction. In this study, we successfully constructed TM3-GAPDS stable strain and investigated the role of sperm specific glyceraldehyde-3-phosphate dehydrogenase (GAPDS) on high glucose-induced apoptosis in TM3 cells. High glucose decreased the protein expression of SOD2 and catalase, while the level of intracellular ROS and the apoptosis - related protein increased in TM3 cells...
June 2, 2018: Biochemical and Biophysical Research Communications
J C E, L Wang, S Chen, Y Y Zhang, S N Luo
GAPD, a graphics-processing-unit (GPU)-accelerated atom-based polychromatic diffraction simulation code for direct, kinematics-based, simulations of X-ray/electron diffraction of large-scale atomic systems with mono-/polychromatic beams and arbitrary plane detector geometries, is presented. This code implements GPU parallel computation via both real- and reciprocal-space decompositions. With GAPD, direct simulations are performed of the reciprocal lattice node of ultralarge systems (∼5 billion atoms) and diffraction patterns of single-crystal and polycrystalline configurations with mono- and polychromatic X-ray beams (including synchrotron undulator sources), and validation, benchmark and application cases are presented...
March 1, 2018: Journal of Synchrotron Radiation
René R Zimmermann, Tobias Hahn, Wladimir Reschetilowski, Marc Armbrüster
Intermetallic GaPd2 is a highly selective and stable catalyst for the semi-hydrogenation of acetylene. Knowledge of the underlying reaction kinetics is essential to gain a deeper understanding of the selective hydrogenation on this catalytic material. To date, there has been no experimental kinetic data published for this reaction on a well-defined intermetallic catalyst possessing isolated active sites. Kinetic measurements are performed at 140-200 °C, revealing an apparent activation energy of 29(2) kJ mol-1 ...
September 20, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
Sebastián Alarcón Villaseca, Alim Ormeci, Sergey V Levchenko, Robert Schlögl, Yuri Grin, Marc Armbrüster
The electron localizability indicator-an efficient quantum chemical tool for analysis of chemical bonding-is applied to unveil the chemical bonding behind the CO adsorption on the (1‾ 1‾ 1‾ ) surface of the highly selective semi-hydrogenation catalyst GaPd. Refining the commonly applied Blyholder model, the obtained results are in excellent agreement with previous experimental and theoretical findings. The clean GaPd(1‾ 1‾ 1‾ ) surface presents unshielded negatively charged Pd centers and positively charged Ga species partially shielded by dangling bonds...
February 17, 2017: Chemphyschem: a European Journal of Chemical Physics and Physical Chemistry
K V Barinova, E V Khomyakova, M L Kuravsky, E V Schmalhausen, V I Muronetz
Influence of the immunization procedure on the specificity of the produced antibodies towards different conformations of the antigen was investigated. It was demonstrated that intravenous immunization of a rabbit with an adjuvant-free solution of recombinant sperm-specific glyceraldehyde-3-phosphate dehydrogenase (dN-GAPDS) resulted in production of antibodies recognizing only native conformation of dN-GAPDS and exhibiting no cross-reaction with somatic isoenzyme of glyceraldehyde-3-phosphate dehydrogenase...
January 22, 2017: Biochemical and Biophysical Research Communications
Jihoon Kang, Yong Choi
BACKGROUND AND OBJECTIVES: Light sharing PET detector configuration coupled with thick light guide and Geiger-mode avalanche photodiode (GAPD) with large-area microcells was proposed to overcome the energy non-linearity problem and to obtain high light collection efficiency (LCE). METHODS: A Monte-Carlo simulation was conducted for the three types of LSO block, 4 × 4 array of 3 × 3 × 20 mm(3) discrete crystals, 6 × 6 array of 2 × 2 × 20 mm(3) discrete crystals, and 12 × 12 array of 1 × 1 × 20 mm(3) discrete crystals, to investigate the scintillation light distribution after conversion of the γ-rays in LSO...
July 2016: Computer Methods and Programs in Biomedicine
S Alarcón Villaseca, S V Levchenko, M Armbrüster
CO adsorption on the polar (1[combining macron]1[combining macron]1[combining macron]) surface of the intermetallic compound GaPd is examined within ab initio methods using an all-electron full-potential electronic structure approach. Comparison between the PW-LDA, GGA-PBE, GGA-RPBE, GGA-revPBE, and hybrid HSE06 functionals is considered through bulk, clean surface and CO adsorption calculations. The choice of the functional is found to have a strong influence in the description of single CO adsorption on the surface model proposed in literature...
June 7, 2016: Physical Chemistry Chemical Physics: PCCP
Y L Elkina, M L Kuravsky, E E Bragina, L F Kurilo, S S Khayat, M Y Sukhomlinova, E V Schmalhausen
The fibrous sheath is a unique cytoskeletal structure surrounding the axoneme and outer dense fibres of the sperm flagellum. Dysplasia of the fibrous sheath (DFS) is a defect of spermatozoa observed in severe asthenozoospermic patients and characterised by morphologically abnormal flagella with distorted fibrous sheaths. Sperm-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDS) is a glycolytic enzyme that is tightly associated with the fibrous sheath of the sperm flagellum. The enzymatic activity of GAPDS was investigated in sperm samples of seven patients with DFS and compared to that of normal spermatozoa (n = 10)...
March 2017: Andrologia
Mirosław Andrusiewicz, Bartosz Słowikowski, Izabela Skibińska, Maria Wołuń-Cholewa, Anna Dera-Szymanowska
PURPOSE: Physiological changes during menstrual cycle cause the endometrium and endometriosis to develop specific kind of tissues, especially in regard to the gene expression profiles, which may include also housekeeping genes, commonly used as reference genes (RGs) in quantitative studies. Reverse transcription, followed by quantitative polymerase chain reaction (RT-qPCR) is the most precise and commonly used method in gene expression studies. In order to reduce effects of technical approaches and biological variability of gene's expression level, the studies often employ RGs in experimental data normalization...
March 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
V I Muronetz, M L Kuravsky, K V Barinova, E V Schmalhausen
This review is focused on the mammalian sperm-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDS). GAPDS plays the major role in the production of energy required for sperm cell movement and does not perform non-glycolytic functions that are characteristic of the somatic isoenzyme of glyceraldehyde-3-phosphate dehydrogenase. The GAPDS sequence is composed of 408 amino acid residues and includes an additional N-terminal region of 72 a.a. that binds the protein to the sperm tail cytoskeleton. GAPDS is present only in the sperm cells of mammals and lizards, possibly providing them with certain evolutionary advantages in reproduction...
December 2015: Biochemistry. Biokhimii︠a︡
Maria Teresa Renzi, Marcel Amichot, David Pauron, Sylvie Tchamitchian, Jean-Luc Brunet, André Kretzschmar, Stefano Maini, Luc P Belzunces
In the agricultural environment, honey bees may be exposed to combinations of pesticides. Until now, the effects of these combinations on honey bee health have been poorly investigated. In this study, we assessed the impacts of biological and chemical insecticides, combining low dietary concentrations of Bacillus thuringiensis (Bt) spores (100 and 1000µg/L) with the chemical insecticide fipronil (1µg/L). In order to assess the possible effects of Cry toxins, the Bt kurstaki strain (Btk) was compared with a Bt strain devoid of toxin-encoding plasmids (Bt Cry(-))...
May 2016: Ecotoxicology and Environmental Safety
V V Evdokimov, K V Barinova, V B Turovetskii, V I Muronetz, E V Schmalhausen
The effect of low concentrations of hydrogen peroxide (10-100 µM) on sperm motility and on the activity of the sperm enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDS) was investigated. Incubation of semen samples with 10 and 100 µM hydrogen peroxide increased the content of spermatozoa with progressive motility by 20 and 18%, respectively, and enhanced the activity of GAPDS in the sperm cells by 27 and 20% compared to a semen sample incubated without additions. It was also found that incubation with 10 µM hydrogen peroxide increased the content of reduced glutathione (GSH) in sperm cells by 50% on average compared to that in the control samples...
September 2015: Biochemistry. Biokhimii︠a︡
Jie Liu, Yilin Wang, Lei Gong, Chengming Sun
The relation between the activity of the sperm specific glyceraldehyde-3-phosphate dehydrogenase (GAPDS) and the motility of sperms in diabetic men was investigated. It was found that the mean value of GAPDS activity and sperm motility in diabetic sperm samples is lower than that in normal samples. The activity of GAPDS protein was significantly positive correlation with sperm motility (R = 0.96) and significantly negative correlation with the incidence of infertility (R = -0.99). The fluorescence intensity of GAPDS protein in sperms of 2-diabetic patients (>5years, n = 88) was significantly lower than that in normal control group...
September 18, 2015: Biochemical and Biophysical Research Communications
Jiao Liu, Haishan Qi, Cheng Wang, Jianping Wen
BACKGROUND: Few strains have been found to produce isobutanol naturally. For building a high performance isobutanol-producing strain, rebalancing redox status of the cell was very crucial through systematic investigation of redox cofactors metabolism. Then, the metabolic model provided a powerful tool for the rational modulation of the redox status. RESULTS: Firstly, a starting isobutanol-producing E. coli strain LA02 was engineered with only 2.7 g/L isobutanol produced...
2015: Biotechnology for Biofuels
Jin Ho Jung, Yong Choi, Jiwoong Jung, Sangsu Kim, Hyun Keong Lim, Ki Chun Im, Chang Hyun Oh, Hyun-wook Park, Kyung Min Kim, Jong Guk Kim
PURPOSE: The purpose of this study was to develop a dual-modality positron emission tomography (PET)/magnetic resonance imaging (MRI) with insertable PET for simultaneous PET and MR imaging of the human brain. METHODS: The PET detector block was composed of a 4 × 4 matrix of detector modules, each consisting of a 4 × 4 array LYSO coupled to a 4 × 4 Geiger-mode avalanche photodiode (GAPD) array. The PET insert consisted of 18 detector blocks, circularly mounted on a custom-made plastic base to form a ring with an inner diameter of 390 mm and axial length of 60 mm...
May 2015: Medical Physics
M L Kuravsky, K V Barinova, R A Asryants, E V Schmalhausen, V I Muronetz
Catalytic properties of enzymes used in biotechnology can be improved by eliminating those regulatory mechanisms that are not absolutely required for their functioning. We exploited mammalian glyceraldehyde-3-phosphate dehydrogenase as a model protein and examined the structural basis of the NAD(+) cooperative binding exhibited by its homologous isoenzymes: the somatic enzyme (GAPD) and the recombinant sperm-specific enzyme (dN-GAPDS). Moreover, we obtained a mutant dN-GAPDS, which misses the cooperativity, but exhibits a twofold increase in the specific activity instead (92 and 45 μmol NADH/min per mg protein for the mutant and the wild type proteins, respectively)...
August 2015: Biochimie
Olga N Makshakova, Pavel I Semenyuk, Mikhail L Kuravsky, Elena A Ermakova, Yuriy F Zuev, Vladimir I Muronetz
Tissue specific isoforms of human glyceraldehyde-3-phosphate dehydrogenase, somatic (GAPD) and sperm-specific (GAPDS), have been reported to display different levels of both stability and catalytic activity. Here we apply MD simulations to investigate molecular basis of this phenomenon. The protein is a tetramer where each subunit consists of two domains - catalytic and NAD-binding one. We demonstrated key residues responsible for intersubunit and interdomain interactions. Effect of several residues was studied by point mutations...
May 2015: Journal of Structural Biology
Jian-Jun Chen, Xiao-Hua Xia, Li-Fang Wang, Yong-Fang Jia, Ping Nan, Li Li, Zhong-Jie Chang
The limited number of gonad-specific and gonad-related genes that have been identified in fish represents a major obstacle in the study of fish gonad development and sex differentiation. In common carp Cyprinus carpio from China's Yellow River, the ovary and testis differ in volume and weight in adult fish of the same age. Comparing sperm, egg, and somatic cell transcripts in this carp may provide insight into the mechanisms of its gonad development and sex differentiation. In the present work, gene expression patterns in the carp ovary and testis were compared using suppression subtractive hybridization...
June 2015: Theriogenology
Karin S Coyne, Brooke M Currie, Christine L Thompson, Ted M Smith
INTRODUCTION: In order to reliably assess treatment effectiveness, patient-reported outcome instruments must demonstrate adequate psychometric properties. AIM: To assess the responsiveness of the Peyronie's Disease Questionnaire (PDQ) using data from two Phase 3 trials of collagenase clostridium histolyticum for Peyronie's disease (PD). METHODS: Both trials recruited adult males with PD who were in a stable relationship with a female partner for at least 3 months...
April 2015: Journal of Sexual Medicine
Johannes Zimmermann, Reiner W Dahlbender, Willy Herbold, Katharina Krasnow, Carmen Meiwes Turrión, Maya Zika, Carsten Spitzer
INTRODUCTION: The alternative DSM-5 model of personality disorder (PD) links general criteria and severity of PD to impairments in self and interpersonal functioning. This approach is very similar to the Level of Structural Impairment Axis of the Operationalized Psychodynamic Diagnosis (OPD-2). In this study we investigate the relationship of these 2 approaches based on 2 new self-report questionnaires. METHODS: 228 psychotherapeutic inpatients filled out the OPD Structure Questionnaire (OPD-SQ), the General Assessment of Personality Disorder (GAPD), and the Patient Health Questionnaire (PHQ-D)...
February 2015: Psychotherapie, Psychosomatik, Medizinische Psychologie
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