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https://www.readbyqxmd.com/read/27265050/simulation-study-of-pet-detector-configuration-with-thick-light-guide-and-gapd-array-having-large-area-microcells-for-high-effective-quantum-efficiency
#1
Jihoon Kang, Yong Choi
BACKGROUND AND OBJECTIVES: Light sharing PET detector configuration coupled with thick light guide and Geiger-mode avalanche photodiode (GAPD) with large-area microcells was proposed to overcome the energy non-linearity problem and to obtain high light collection efficiency (LCE). METHODS: A Monte-Carlo simulation was conducted for the three types of LSO block, 4 × 4 array of 3 × 3 × 20 mm(3) discrete crystals, 6 × 6 array of 2 × 2 × 20 mm(3) discrete crystals, and 12 × 12 array of 1 × 1 × 20 mm(3) discrete crystals, to investigate the scintillation light distribution after conversion of the γ-rays in LSO...
July 2016: Computer Methods and Programs in Biomedicine
https://www.readbyqxmd.com/read/27223480/co-adsorption-on-the-gapd-1-combining-macron-1-combining-macron-1-combining-macron-surface-a-comparative-dft-study-using-different-functionals
#2
S Alarcón Villaseca, S V Levchenko, M Armbrüster
CO adsorption on the polar (1[combining macron]1[combining macron]1[combining macron]) surface of the intermetallic compound GaPd is examined within ab initio methods using an all-electron full-potential electronic structure approach. Comparison between the PW-LDA, GGA-PBE, GGA-RPBE, GGA-revPBE, and hybrid HSE06 functionals is considered through bulk, clean surface and CO adsorption calculations. The choice of the functional is found to have a strong influence in the description of single CO adsorption on the surface model proposed in literature...
June 7, 2016: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/27135296/detection-of-a-mutation-in-the-intron-of-sperm-specific-glyceraldehyde-3-phosphate-dehydrogenase-gene-in-patients-with-fibrous-sheath-dysplasia-of-the-sperm-flagellum
#3
Y L Elkina, M L Kuravsky, E E Bragina, L F Kurilo, S S Khayat, M Y Sukhomlinova, E V Schmalhausen
The fibrous sheath is a unique cytoskeletal structure surrounding the axoneme and outer dense fibres of the sperm flagellum. Dysplasia of the fibrous sheath (DFS) is a defect of spermatozoa observed in severe asthenozoospermic patients and characterised by morphologically abnormal flagella with distorted fibrous sheaths. Sperm-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDS) is a glycolytic enzyme that is tightly associated with the fibrous sheath of the sperm flagellum. The enzymatic activity of GAPDS was investigated in sperm samples of seven patients with DFS and compared to that of normal spermatozoa (n = 10)...
May 2, 2016: Andrologia
https://www.readbyqxmd.com/read/26898426/selection-of-reliable-reference-genes-in-eutopic-and-ectopic-endometrium-for-quantitative-expression-studies
#4
Mirosław Andrusiewicz, Bartosz Słowikowski, Izabela Skibińska, Maria Wołuń-Cholewa, Anna Dera-Szymanowska
PURPOSE: Physiological changes during menstrual cycle cause the endometrium and endometriosis to develop specific kind of tissues, especially in regard to the gene expression profiles, which may include also housekeeping genes, commonly used as reference genes (RGs) in quantitative studies. Reverse transcription, followed by quantitative polymerase chain reaction (RT-qPCR) is the most precise and commonly used method in gene expression studies. In order to reduce effects of technical approaches and biological variability of gene's expression level, the studies often employ RGs in experimental data normalization...
March 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/26878573/sperm-specific-glyceraldehyde-3-phosphate-dehydrogenase-an-evolutionary-acquisition-of-mammals
#5
REVIEW
V I Muronetz, M L Kuravsky, K V Barinova, E V Schmalhausen
This review is focused on the mammalian sperm-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDS). GAPDS plays the major role in the production of energy required for sperm cell movement and does not perform non-glycolytic functions that are characteristic of the somatic isoenzyme of glyceraldehyde-3-phosphate dehydrogenase. The GAPDS sequence is composed of 408 amino acid residues and includes an additional N-terminal region of 72 a.a. that binds the protein to the sperm tail cytoskeleton. GAPDS is present only in the sperm cells of mammals and lizards, possibly providing them with certain evolutionary advantages in reproduction...
December 2015: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/26866756/chronic-toxicity-and-physiological-changes-induced-in-the-honey-bee-by-the-exposure-to-fipronil-and-bacillus-thuringiensis-spores-alone-or-combined
#6
Maria Teresa Renzi, Marcel Amichot, David Pauron, Sylvie Tchamitchian, Jean-Luc Brunet, André Kretzschmar, Stefano Maini, Luc P Belzunces
In the agricultural environment, honey bees may be exposed to combinations of pesticides. Until now, the effects of these combinations on honey bee health have been poorly investigated. In this study, we assessed the impacts of biological and chemical insecticides, combining low dietary concentrations of Bacillus thuringiensis (Bt) spores (100 and 1000µg/L) with the chemical insecticide fipronil (1µg/L). In order to assess the possible effects of Cry toxins, the Bt kurstaki strain (Btk) was compared with a Bt strain devoid of toxin-encoding plasmids (Bt Cry(-))...
May 2016: Ecotoxicology and Environmental Safety
https://www.readbyqxmd.com/read/26555470/low-concentrations-of-hydrogen-peroxide-activate-the-antioxidant-defense-system-in-human-sperm-cells
#7
V V Evdokimov, K V Barinova, V B Turovetskii, V I Muronetz, E V Schmalhausen
The effect of low concentrations of hydrogen peroxide (10-100 µM) on sperm motility and on the activity of the sperm enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDS) was investigated. Incubation of semen samples with 10 and 100 µM hydrogen peroxide increased the content of spermatozoa with progressive motility by 20 and 18%, respectively, and enhanced the activity of GAPDS in the sperm cells by 27 and 20% compared to a semen sample incubated without additions. It was also found that incubation with 10 µM hydrogen peroxide increased the content of reduced glutathione (GSH) in sperm cells by 50% on average compared to that in the control samples...
September 2015: Biochemistry. Biokhimii︠a︡
https://www.readbyqxmd.com/read/26255202/oxidation-of-glyceraldehyde-3-phosphate-dehydrogenase-decreases-sperm-motility-in-diabetes-mellitus
#8
Jie Liu, Yilin Wang, Lei Gong, Chengming Sun
The relation between the activity of the sperm specific glyceraldehyde-3-phosphate dehydrogenase (GAPDS) and the motility of sperms in diabetic men was investigated. It was found that the mean value of GAPDS activity and sperm motility in diabetic sperm samples is lower than that in normal samples. The activity of GAPDS protein was significantly positive correlation with sperm motility (R = 0.96) and significantly negative correlation with the incidence of infertility (R = -0.99). The fluorescence intensity of GAPDS protein in sperms of 2-diabetic patients (>5years, n = 88) was significantly lower than that in normal control group...
September 18, 2015: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/26236397/model-driven-intracellular-redox-status-modulation-for-increasing-isobutanol-production-in-escherichia-coli
#9
Jiao Liu, Haishan Qi, Cheng Wang, Jianping Wen
BACKGROUND: Few strains have been found to produce isobutanol naturally. For building a high performance isobutanol-producing strain, rebalancing redox status of the cell was very crucial through systematic investigation of redox cofactors metabolism. Then, the metabolic model provided a powerful tool for the rational modulation of the redox status. RESULTS: Firstly, a starting isobutanol-producing E. coli strain LA02 was engineered with only 2.7 g/L isobutanol produced...
2015: Biotechnology for Biofuels
https://www.readbyqxmd.com/read/25979030/development-of-pet-mri-with-insertable-pet-for-simultaneous-pet-and-mr-imaging-of-human-brain
#10
Jin Ho Jung, Yong Choi, Jiwoong Jung, Sangsu Kim, Hyun Keong Lim, Ki Chun Im, Chang Hyun Oh, Hyun-wook Park, Kyung Min Kim, Jong Guk Kim
PURPOSE: The purpose of this study was to develop a dual-modality positron emission tomography (PET)/magnetic resonance imaging (MRI) with insertable PET for simultaneous PET and MR imaging of the human brain. METHODS: The PET detector block was composed of a 4 × 4 matrix of detector modules, each consisting of a 4 × 4 array LYSO coupled to a 4 × 4 Geiger-mode avalanche photodiode (GAPD) array. The PET insert consisted of 18 detector blocks, circularly mounted on a custom-made plastic base to form a ring with an inner diameter of 390 mm and axial length of 60 mm...
May 2015: Medical Physics
https://www.readbyqxmd.com/read/25936797/structural-basis-for-the-nad-binding-cooperativity-and-catalytic-characteristics-of-sperm-specific-glyceraldehyde-3-phosphate-dehydrogenase
#11
M L Kuravsky, K V Barinova, R A Asryants, E V Schmalhausen, V I Muronetz
Catalytic properties of enzymes used in biotechnology can be improved by eliminating those regulatory mechanisms that are not absolutely required for their functioning. We exploited mammalian glyceraldehyde-3-phosphate dehydrogenase as a model protein and examined the structural basis of the NAD(+) cooperative binding exhibited by its homologous isoenzymes: the somatic enzyme (GAPD) and the recombinant sperm-specific enzyme (dN-GAPDS). Moreover, we obtained a mutant dN-GAPDS, which misses the cooperativity, but exhibits a twofold increase in the specific activity instead (92 and 45 μmol NADH/min per mg protein for the mutant and the wild type proteins, respectively)...
August 2015: Biochimie
https://www.readbyqxmd.com/read/25869789/structural-basis-for-regulation-of-stability-and-activity-in-glyceraldehyde-3-phosphate-dehydrogenases-differential-scanning-calorimetry-and-molecular-dynamics
#12
Olga N Makshakova, Pavel I Semenyuk, Mikhail L Kuravsky, Elena A Ermakova, Yuriy F Zuev, Vladimir I Muronetz
Tissue specific isoforms of human glyceraldehyde-3-phosphate dehydrogenase, somatic (GAPD) and sperm-specific (GAPDS), have been reported to display different levels of both stability and catalytic activity. Here we apply MD simulations to investigate molecular basis of this phenomenon. The protein is a tetramer where each subunit consists of two domains - catalytic and NAD-binding one. We demonstrated key residues responsible for intersubunit and interdomain interactions. Effect of several residues was studied by point mutations...
May 2015: Journal of Structural Biology
https://www.readbyqxmd.com/read/25772851/identification-and-comparison-of-gonadal-transcripts-of-testis-and-ovary-of-adult-common-carp-cyprinus-carpio-using-suppression-subtractive-hybridization
#13
Jian-Jun Chen, Xiao-Hua Xia, Li-Fang Wang, Yong-Fang Jia, Ping Nan, Li Li, Zhong-Jie Chang
The limited number of gonad-specific and gonad-related genes that have been identified in fish represents a major obstacle in the study of fish gonad development and sex differentiation. In common carp Cyprinus carpio from China's Yellow River, the ovary and testis differ in volume and weight in adult fish of the same age. Comparing sperm, egg, and somatic cell transcripts in this carp may provide insight into the mechanisms of its gonad development and sex differentiation. In the present work, gene expression patterns in the carp ovary and testis were compared using suppression subtractive hybridization...
June 2015: Theriogenology
https://www.readbyqxmd.com/read/25664497/responsiveness-of-the-peyronie-s-disease-questionnaire-pdq
#14
Karin S Coyne, Brooke M Currie, Christine L Thompson, Ted M Smith
INTRODUCTION: In order to reliably assess treatment effectiveness, patient-reported outcome instruments must demonstrate adequate psychometric properties. AIM: To assess the responsiveness of the Peyronie's Disease Questionnaire (PDQ) using data from two Phase 3 trials of collagenase clostridium histolyticum for Peyronie's disease (PD). METHODS: Both trials recruited adult males with PD who were in a stable relationship with a female partner for at least 3 months...
April 2015: Journal of Sexual Medicine
https://www.readbyqxmd.com/read/25503590/-the-opd-structure-questionnaire-captures-the-general-features-of-personality-disorder
#15
Johannes Zimmermann, Reiner W Dahlbender, Willy Herbold, Katharina Krasnow, Carmen Meiwes Turrión, Maya Zika, Carsten Spitzer
INTRODUCTION: The alternative DSM-5 model of personality disorder (PD) links general criteria and severity of PD to impairments in self and interpersonal functioning. This approach is very similar to the Level of Structural Impairment Axis of the Operationalized Psychodynamic Diagnosis (OPD-2). In this study we investigate the relationship of these 2 approaches based on 2 new self-report questionnaires. METHODS: 228 psychotherapeutic inpatients filled out the OPD Structure Questionnaire (OPD-SQ), the General Assessment of Personality Disorder (GAPD), and the Patient Health Questionnaire (PHQ-D)...
February 2015: Psychotherapie, Psychosomatik, Medizinische Psychologie
https://www.readbyqxmd.com/read/25308277/chemical-targeting-of-gapdh-moonlighting-function-in-cancer-cells-reveals-its-role-in-tubulin-regulation
#16
Da-Woon Jung, Woong-Hee Kim, Shinae Seo, Eunsang Oh, Soon-Ho Yim, Hyung-Ho Ha, Young-Tae Chang, Darren Reece Williams
Glycolytic enzymes are attractive anticancer targets. They also carry out numerous, nonglycolytic "moonlighting" functions in cells. In this study, we investigated the anticancer activity of the triazine small molecule, GAPDS, that targets the glycolytic enzyme glyceraldehyde 3-phosphate dehydrogenase (GAPDH). GAPDS showed greater toxicity against cancer cells compared to a known GAPDH enzyme inhibitor. GAPDS also selectively inhibited cell migration and invasion. Our analysis showed that GAPDS treatment reduced GAPDH levels in the cytoplasm, which would modulate the secondary, moonlighting functions of this enzyme...
November 20, 2014: Chemistry & Biology
https://www.readbyqxmd.com/read/25091199/sperm-specific-glyceraldehyde-3-phosphate-dehydrogenase-is-stabilized-by-additional-proline-residues-and-an-interdomain-salt-bridge
#17
Mikhail Kuravsky, Kseniya Barinova, Aleksandra Marakhovskaya, Mikhail Eldarov, Pavel Semenyuk, Vladimir Muronetz, Elena Schmalhausen
Sperm-specific glyceraldehyde-3-phosphate dehydrogenase (GAPDS) exhibits enhanced stability compared to the somatic isoenzyme (GAPD). A comparative analysis of the structures of these isoenzymes revealed characteristic features, which could be important for the stability of GAPDS: six specific proline residues and three buried salt bridges. To evaluate the impact of these structural elements into the stability of this isoenzyme, we obtained two series of mutant GAPDS: 1) six mutants each containing a substitution of one of the specific prolines by alanine, and 2) three mutants each containing a mutation breaking one of the salt bridges...
October 2014: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/25081451/on-the-reclassification-of-the-terrapene-testudines-emydidae-a-response-to-fritz-hava%C3%A5
#18
LETTER
Bradley T Martin, Neil P Bernstein, Roger D Birkhead, Jim F Koukl, Steven M Mussmann, John S Placyk
No abstract text is available yet for this article.
2014: Zootaxa
https://www.readbyqxmd.com/read/24831709/optimal-cofactor-swapping-can-increase-the-theoretical-yield-for-chemical-production-in-escherichia-coli-and-saccharomyces-cerevisiae
#19
Zachary A King, Adam M Feist
Maintaining cofactor balance is a critical function in microorganisms, but often the native cofactor balance does not match the needs of an engineered metabolic flux state. Here, an optimization procedure is utilized to identify optimal cofactor-specificity "swaps" for oxidoreductase enzymes utilizing NAD(H) or NADP(H) in the genome-scale metabolic models of Escherichia coli and Saccharomyces cerevisiae. The theoretical yields of all native carbon-containing molecules are considered, as well as theoretical yields of twelve heterologous production pathways in E...
July 2014: Metabolic Engineering
https://www.readbyqxmd.com/read/24741993/surfaces-of-complex-intermetallic-compounds-insights-from-density-functional-calculations
#20
Jürgen Hafner, Marian Krajčí
CONSPECTUS: Complex intermetallic compounds are a class of ordered alloys consisting of quasicrystals and other ordered compounds with large unit cells; many of them are approximant phases to quasicrystals. Quasicrystals are the limiting case where the unit cell becomes infinitely large; approximants are series of periodic structures converging to the quasicrystal. While the unique properties of quasicrystals have inspired many investigations of their surfaces, relatively little attention has been devoted to the surface properties of the approximants...
November 18, 2014: Accounts of Chemical Research
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