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bio chemistry

Xiaotong Cao, Jianquan Luo, John M Woodley, Yinhua Wan
Micro-pollutants present in water have many detrimental effects on the ecosystem. Membrane technology plays an important role in the removal of micro-pollutants but there remain significant challenges such as concentration polarization, membrane fouling and variable permeate quality. The work reported here uses a multifunctional membrane with rejection, adsorption and catalysis functions to solve these problems. Based on mussel-inspired chemistry and biological membrane properties, a multifunctional membrane was prepared by applying 'reverse filtration' of a laccase solution and subsequent 'dopamine coating' on a nanofiltration (NF) membrane support, which was tested on bisphenol A (BPA) removal...
October 21, 2016: ACS Applied Materials & Interfaces
Carla Ferreri, Bernard T Golding, Ullrich Jahn, Jean-Luc Ravanat
The COST Action CM1201 "Biomimetic Radical Chemistry" has been active since December 2012 for four years, developing research topics organized into four working groups: WG1 - Radical Enzymes, WG2 - Models of DNA damage and consequences, WG3 - Membrane stress, signalling and defences, WG4 - Bio-inspired synthetic strategies. International collaborations have been established among the participating 80 research groups with brilliant interdisciplinary achievements. Free radical research with a biomimetic approach has been realized in the COST Action and are summarized in this overview by the four WG leaders...
October 17, 2016: Free Radical Research
Sasanka Dalapati, Cheng Gu, Donglin Jiang
Enormous research efforts are focusing on the design and synthesis of advanced luminescent systems, owing to their diverse capability in scientific studies and technological developments. In particular, fluorescence systems based on aggregation-induced emission (AIE) have emerged to show great potential for sensing, bio-imaging, and optoelectronic applications. Among them, integrating AIE mechanisms to design porous polymers is unique because it enables the combination of porosity and luminescence activity in one molecular skeleton for functional design...
October 14, 2016: Small
Jorin Hoogenboom, Nathalja Berghuis, Dario Cramer, Rene Geurts, Han Zuilhof, Tom Wennekes
BACKGROUND: Carbohydrates, also called glycans, play a crucial but not fully understood role in plant health and development. The non-template driven formation of glycans makes it impossible to image them in vivo with genetically encoded fluorescent tags and related molecular biology approaches. A solution to this problem is the use of tailor-made glycan analogs that are metabolically incorporated by the plant into its glycans. These metabolically incorporated probes can be visualized, but techniques documented so far use toxic copper-catalyzed labeling...
October 10, 2016: BMC Plant Biology
Xiaowen Yan, Jinhua Li, Qingqing Liu, Hanyong Peng, Aleksandra Popowich, Zhixin Wang, Xing-Fang Li, X Chris Le
Identification of arsenic-binding proteins is important for understanding arsenic health effects and for developing arsenic-based therapeutics. We report here a strategy for the capture and identification of arsenic-binding proteins in living cells. We designed an azide-labeled arsenical, p-azidophenylarsenoxide (PAzPAO), to serve bio-orthogonal functions: the trivalent arsenical group binds to cellular proteins in situ, and the azide group facilitates click chemistry with dibenzylcyclooctyne. The selective and efficient capture of arsenic-binding proteins enables subsequent enrichment and identification by shotgun proteomics...
October 10, 2016: Angewandte Chemie
Mélanie Flaender, Guillaume Costa, Guillaume Nonglaton, Christine Saint-Pierre, Didier Gasparutto
DNA is under continuous assault by environmental and endogenous reactive oxygen and alkylating species, inducing the formation of mutagenic, toxic and genome destabilizing nucleobase lesions. Due to the implications of such genetic alterations in cell death, aging, inflammation, neurodegenerative diseases and cancer, many efforts have been devoted to developing assays that aim at analyzing DNA repair activities from purified enzymes or cell extracts. The present work deals with the conception and application of a new, miniaturized and parallelized on surface-DNA biosensor to measure base excision repair (BER) activities...
September 22, 2016: Analyst
Sebastian Mai, Marvin Pollum, Lara Martínez-Fernández, Nicholas Dunn, Philipp Marquetand, Inés Corral, Carlos E Crespo-Hernández, Leticia González
Elucidating the photophysical mechanisms in sulfur-substituted nucleobases (thiobases) is essential for designing prospective drugs for photo- and chemotherapeutic applications. Although it has long been established that the phototherapeutic activity of thiobases is intimately linked to efficient intersystem crossing into reactive triplet states, the molecular factors underlying this efficiency are poorly understood. Herein we combine femtosecond transient absorption experiments with quantum chemistry and nonadiabatic dynamics simulations to investigate 2-thiocytosine as a necessary step to unravel the electronic and structural elements that lead to ultrafast and near-unity triplet-state population in thiobases in general...
October 5, 2016: Nature Communications
Si Yeon Lee, Sangmin Lee, Jangwook Lee, Ji Young Yhee, Hwa In Yoon, Soon-Jung Park, Heebeom Koo, Sung-Hwan Moon, Hyukjin Lee, Yong Woo Cho, Sun Woong Kang, Sang-Yup Lee, Kwangmeyung Kim
Labeling of stem cells aims to distinguish transplanted cells from host cells, understand in vivo fate of transplanted cells, particularly important in stem cell therapy. Adipose-derived mesenchymal stem cells (ASCs) are considered as an emerging therapeutic option for tissue regeneration, but much remains to be understood regarding the in vivo evidence. In this study, a simple and efficient cell labeling method for labeling and tracking of stem cells was developed based on bio-orthogonal copper-free click chemistry, and it was applied in a mouse hindlimb ischemia model...
October 28, 2016: Biochemical and Biophysical Research Communications
Mahkameh Amirbandeh, Asghar Taheri-Kafrani
Immobilization of an enzyme can enhance its catalytic activity, depending on the properties of the enzyme and the matrix. Graphene oxide is a nontoxic material and selective modulator for enzyme activity and is also a thermostable molecule that is important in large-scale nanostructure sheet applications. Herein, we have successfully developed a strategy for preparing a nanocomposite for enzyme immobilization model with high loading capacity. Nanostructures of hybrid graphene oxide-Fe3O4-cyanuric chloride (GO/MNP-CC) have adjustable surface chemistry that is an excellent candidate for covalent immobilization of enzymes...
September 28, 2016: International Journal of Biological Macromolecules
Xiang Gao, Jinlin Song, Yancong Zhang, Xiao Xu, Siqi Zhang, Ping Ji, Shicheng Wei
The design and development of functional biomimetic systems for programmed stem cell response is a field of topical interest. To mimic bone extracellular matrix, we present an innovative strategy for constructing drug-loaded composite nanofibrous scaffolds in this study, which could integrate multiple cues from calcium phosphate mineral, bioactive molecule, and highly ordered fiber topography for the control of stem cell fate. Briefly, inspired by mussel adhesion mechanism, a polydopamine (pDA)-templated nanohydroxyapatite (tHA) was synthesized and then surface-functionalized with bone morphogenetic protein-7-derived peptides via catechol chemistry...
October 7, 2016: ACS Applied Materials & Interfaces
Banani Kundu, Mohamed Eltohamy, Vamsi K Yadavalli, Subhas C Kundu, Hae-Won Kim
In nature inorganic-organic building units create multifunctional hierarchical architectures. Organic silk protein is particularly attractive in this respect because of its micro-nanoscale structural blocks that are attributed to sophisticated hierarchical assembly imparting flexibility and compressibility to designed biohybrid materials. In the present study, aqueous silk fibroin is assembled to form nano/microtopography on inorganic silica surface via a facile diffusion-limited aggregation process. This process is driven by electrostatic interaction and only possible at a specified aminated surface chemistry...
October 12, 2016: ACS Applied Materials & Interfaces
Christopher M Madl, Lily M Katz, Sarah C Heilshorn
Covalently-crosslinked hydrogels are commonly used as 3D matrices for cell culture and transplantation. However, the crosslinking chemistries used to prepare these gels generally cross-react with functional groups present on the cell surface, potentially leading to cytotoxicity and other undesired effects. Bio-orthogonal chemistries have been developed that do not react with biologically relevant functional groups, thereby preventing these undesirable side reactions. However, previously developed biomaterials using these chemistries still possess less than ideal properties for cell encapsulation, such as slow gelation kinetics and limited tuning of matrix mechanics and biochemistry...
June 7, 2016: Advanced Functional Materials
Silvia Tejada, Azadeh Manayi, Maria Daglia, Seyed F Nabavi, Antoni Sureda, Zohreh Hajheydari, Olga Gortzi, Hamidreza Pazoki-Toroudi, Seyed M Nabavi
Wound healing is a complex process that consists of several phases that range from coagulation, inflammation, accumulation of radical substances, to proliferation, formation of fibrous tissues and collagen, contraction of wound with formation of granulation tissue and scar. Since antiquity, vegetable substances have been used as phytotherapeutic agents for wound healing, and more recently natural substances of vegetable origin have been studied with the attempt to show their beneficial effect on wound treatment...
2016: Current Pharmaceutical Biotechnology
Arunpandian Balaji, Saravana Kumar Jaganathan, Ahmad Fauzi Ismail, Rathanasamy Rajasekar
Management of burn injury is an onerous clinical task since it requires continuous monitoring and extensive usage of specialized facilities. Despite rapid improvizations and investments in burn management, >30% of victims hospitalized each year face severe morbidity and mortality. Excessive loss of body fluids, accumulation of exudate, and the development of septic shock are reported to be the main reasons for morbidity in burn victims. To assist burn wound management, a novel polyurethane (PU)-based bio-nanofibrous dressing loaded with honey (HN) and Carica papaya (PA) fruit extract was fabricated using a one-step electrospinning technique...
2016: International Journal of Nanomedicine
Sung-Tae Yang, Sung In Lim, Volker Kiessling, Inchan Kwon, Lukas K Tamm
Fluorescence approaches have been widely used for elucidating the dynamics of protein-membrane interactions in cells and model systems. However, non-specific multi-site fluorescent labeling often results in a loss of native structure and function, and single cysteine labeling is not feasible when native cysteines are required to support a protein's folding or catalytic activity. Here, we develop a method using genetic incorporation of non-natural amino acids and bio-orthogonal chemistry to site-specifically label with a single fluorescent small molecule or protein the myristoyl-switch protein recoverin, which is involved in rhodopsin-mediated signaling in mammalian visual sensory neurons...
2016: Scientific Reports
Xinxin Gao, Rami N Hannoush
Wnt palmitoylation regulates its secretion and signaling activity in cells. Methods to monitor cellular Wnt palmitoylation are instrumental in investigating Wnt activity, secretion, and its interaction with cellular membrane compartments. This protocol describes a method we have recently developed to detect cellular Wnt palmitoylation. The method, combining click chemistry, bio-orthogonal fatty acid probes, and proximity ligation assay (PLA), provides high sensitivity and subcellular resolution for detection of Wnt palmitoylation...
2016: Methods in Molecular Biology
Kyung Hoon Kim, Jae-Min Jeong, Seok Jae Lee, Bong Gill Choi, Kyoung G Lee
The understanding and controlling of biomimetic hybrid materials are a key objective in bio-nanotechnology, materials chemistry, and colloid science fields. Biomaterials, such as, enzyme, DNA, RNA, and proteins have become important templates for the construction of inorganic-organic hybrid nanoflowers. From this perspective, we present a simple approach to synthesize protein and metal hybrid flower-like structure using bovine serum albumin (BSA) and cobalt phosphate, and the results of our study on the formation mechanism involved...
December 15, 2016: Journal of Colloid and Interface Science
Elise Billès, Kelechukwu N Onwukamike, Véronique Coma, Stéphane Grelier, Frédéric Peruch
Cellulose oligomers are water-soluble, on the contrary to cellulose, which greatly increase their application range. In this study, cellulose oligomers were obtained from the acidic hydrolysis of cellulose with phosphoric acid. The global yield in water-soluble oligomers was around 23% with polymerization degree (DP) ranging from 1 to 12. The cellulose oligomers DP distribution was successfully reduced by differential solubilisation in methanol as one of the goals of this work was to avoid the use of a time-consuming full chromatographic separation...
December 10, 2016: Carbohydrate Polymers
Romain Aufaure, Julie Hardouin, Nadine Millot, Laurence Motte, Yoann Lalatonne, Erwann Guénin
Inverse electron demand Diels-Alder (iEDDA) was evaluated for the functionalization of gold nanoparticles. The reaction was first modelled with the free coating molecule 1-hydroxy-1,1-methylenebisphosphonate bearing an alkene functionality (HMBPene). A model tetrazine 3,6-dipyridin-2-yl-1,2,4,5-tetrazine (pyTz) was used, kinetic of the reaction was calculated and coupling products were analysed by NMR and HRMS. The reaction was then transposed at the nanoparticle surface. Gold nanoparticles bearing an alkene functionality were obtained using a one-pot methodology with HMBPene and the tetrazine click chemistry was evaluated at their surface using pyTz...
November 2, 2016: Chemistry: a European Journal
Sedigheh Basiri, Ali Mehdinia, Ali Jabbari
A highly sensitive and cost-effective colorimetric sensing platform for the selective trace analysis of Cu(2+) ions was developed based on the accelerated etching of Riboflavin stabilized silver nanoparticles (R/AgNPs). The R/AgNPs were prepared from the Cucumis melo juice by a green chemistry approach. The bio-synthesized AgNPs were studied by UV-Vis spectroscopy and showed an intense absorption band at 404nm that were further confirmed by FTIR and EDS analysis. Simultaneous presence of Cu(2+) and thiosulfate decreased the absorption intensity of green synthesized AgNPs which resulted in sensitive and selective determination of Cu(2+)...
January 15, 2017: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
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