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https://www.readbyqxmd.com/read/27925692/a-method-to-generate-and-analyze-modified-myristoylated-proteins
#1
Huanyao Gao, Wei Sun, Zhiquan Song, Yanbao Yu, Li Wang, Xian Chen, Qisheng Zhang
Covalent lipid modification of proteins is essential to their cellular localizations and functions. Engineered lipid motif coupled with bio-orthogonal chemistry has been utilized to identify myristoylated or palmitoylated proteins in cells. However, whether modified proteins have similar properties as endogenous ones has not been well investigated mainly due to lack of methods to generate and analyze purified proteins. We have developed a method that utilizes metabolic interference and mass spectrometry to produce and analyze modified, myristoylated small GTPase ADP-ribosylation factor 1 (Arf1)...
December 7, 2016: Chembiochem: a European Journal of Chemical Biology
https://www.readbyqxmd.com/read/27924490/site-specific-fluorescent-labeling-of-argonaute-for-fret-based-bio-assays
#2
Sarah Willkomm, Adrian Zander, Dina Grohmann
Deciphering the molecular mechanisms of eukaryotic Argonaute proteins is crucial for the understanding of RNA interference (RNAi), a posttranscriptional gene silencing process. Fluorescence-based single-molecule studies like single-molecule Förster resonance energy transfer (FRET) between a donor and acceptor dye represent a versatile tool to gain a mechanistic understanding of the structural dynamics of a biomolecular complex. Until today it was not possible to site-specifically introduce fluorophores into eukaryotic Argonaute...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/27915023/anisotropic-noble-metal-nanoparticles-synthesis-surface-functionalization-and-applications-in-biosensing-bioimaging-drug-delivery-and-theranostics
#3
REVIEW
Gokul Paramasivam, Namitharan Kayambu, Arul Maximus Rabel, Ashok K Sundramoorthy, Anandhakumar Sundaramurthy
Anisotropic nanoparticles have fascinated scientists and engineering communities for over a century because of their unique physical and chemical properties. In recent years, continuous advances in design and fabrication of anisotropic nanoparticles have opened new avenues for application in various areas of biology, chemistry and physics. Anisotropic nanoparticles have the plasmon absorption in the visible as well as near-infrared (NIR) region, which enable them to be used for crucial applications such as biological imaging, medical diagnostics and therapy ("theranostics")...
November 30, 2016: Acta Biomaterialia
https://www.readbyqxmd.com/read/27904956/some-nontoxic-metal-based-drugs-for-selected-prevalent-tropical-pathogenic-diseases
#4
REVIEW
Saliu A Amolegbe, Caroline A Akinremi, Sheriff Adewuyi, Amudat Lawal, Mercy O Bamigboye, Joshua A Obaleye
Metal coordination of bioorganic compounds from both natural and synthetic products is not only gaining recognition in drug design and medicinal inorganic chemistry research, also they are being considered in the improvement of the bioactivity of drugs. What is done in this paper is a review of recent advances in the study of coordination-driven drug delivery, i.e., metal-based drugs (MBDs). The role of some late first row transition metal ions namely Fe, Cu and Zn in the biological activities of metallodrugs such as antimalarials and antimicrobials are highlighted...
November 30, 2016: Journal of Biological Inorganic Chemistry: JBIC
https://www.readbyqxmd.com/read/27877242/nanoscale-fluorescent-stone-luminescent-calcium-fluoride-nanoparticles-as-theranostic-platforms
#5
Zhanjun Li, Yuanwei Zhang, Ling Huang, Yuchen Yang, Yang Zhao, Ghida El-Banna, Gang Han
Calcium Fluoride (CaF2) based luminescent nanoparticles exhibit unique, outstanding luminescent properties, and represent promising candidates as nanoplatforms for theranostic applications. There is an urgent need to facilitate their further development and applications in diagnostics and therapeutics as a novel class of nanotools. Here, in this critical review, we outlined the recent significant progresses made in CaF2-related nanoparticles: Firstly, their physical chemical properties, synthesis chemistry, and nanostructure fabrication are summarized...
2016: Theranostics
https://www.readbyqxmd.com/read/27876144/in-tip-nanoreactors-for-cancer-cells-proteome-profiling
#6
Ling Yan, Liang Qiao, Ji Ji, Yixin Li, Xuefei Yin, Ling Lin, Xiaohui Liu, Jun Yao, Yi Wang, Bin Liu, Kun Qian, Baohong Liu, Pengyuan Yang
Mass spectrometry (MS)-based proteome profiling is essential for molecular diagnostics in modern biomedical study. To date, sample preparation including protein extraction and proteolysis is still very challenging and lack of efficiency. Recently tips-based sample preparation protocols exhibit strong potentials to achieve the goal of "a proteome in an hour". However, in-tip proteolysis is still rarely reported and far from ideal for dealing with complex bio-samples. In this work, nanoreactors encapsulated micropipette tips were demonstrated as high performance devices for fast (∼minutes) and multiplexing proteolysis to assist the profiling of cancer cells proteome...
January 1, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/27871373/design-and-production-of-antibodies-for-the-detection-of-streptococcus-uberis
#7
K Mihklepp, K Kivirand, M Nikopensius, D Peedel, M Utt, T Rinken
Streptococcus uberis (S. uberis) is an important environmental pathogen causing mastitis in dairy cattle. Mastitis or mammary gland infection is the most common disease in milking cows and cause significant economic burden to farmers due to the reduction of the amount of milk and its quality. The development of rapid analytical methods for the determination of mastitis-causing pathogens in milk is of utmost importance for the early identification of the causes of mastitis and the beginning of timely treatment of cows...
January 2017: Enzyme and Microbial Technology
https://www.readbyqxmd.com/read/27853966/remediation-of-cu-metal-induced-accelerated-fenton-reaction-by-potato-peels-bio-sorbent
#8
Rafia Azmat, Sumeira Moin, Ailyan Saleem
This article has allied exposure to Ecological Particulate Matter (EPM) and its remediation using potato peel surface (PPC) bio-sorbent on two important edible crops Spinacia oleracea and Luffa acutangula. Fenton reaction acceleration was one of the major stress oxidation reactions as a consequence of iron and copper toxicity, which involve in the formation of hydroxyl radical (OH) through EPM. Results showed that the oxidative stress encouraged by Cu in both species that recruits the degradation of photosynthetic pigments, initiating decline in growth, reduced leaf area and degrade proteins...
December 0: Environmental Monitoring and Assessment
https://www.readbyqxmd.com/read/27834835/large-scale-screening-of-southern-african-plant-extracts-for-the-green-synthesis-of-gold-nanoparticles-using-microtitre-plate-method
#9
Abdulrahman M Elbagory, Christopher N Cupido, Mervin Meyer, Ahmed A Hussein
The preparation of gold nanoparticles (AuNPs) involves a variety of chemical and physical methods. These methods use toxic and environmentally harmful chemicals. Consequently, the synthesis of AuNPs using green chemistry has been under investigation to develop eco-friendly nanoparticles. One approach to achieve this is the use of plant-derived phytochemicals that are capable of reducing gold ions to produce AuNPs. The aim of this study was to implement a facile microtitre-plate method to screen a large number of aqueous plant extracts to determine the optimum concentration (OC) for the bio-synthesis of the AuNPs...
November 8, 2016: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/27834398/cancer-nanomedicine-progress-challenges-and-opportunities
#10
Jinjun Shi, Philip W Kantoff, Richard Wooster, Omid C Farokhzad
The intrinsic limits of conventional cancer therapies prompted the development and application of various nanotechnologies for more effective and safer cancer treatment, herein referred to as cancer nanomedicine. Considerable technological success has been achieved in this field, but the main obstacles to nanomedicine becoming a new paradigm in cancer therapy stem from the complexities and heterogeneity of tumour biology, an incomplete understanding of nano-bio interactions and the challenges regarding chemistry, manufacturing and controls required for clinical translation and commercialization...
November 11, 2016: Nature Reviews. Cancer
https://www.readbyqxmd.com/read/27825880/ratiometric-sensing-of-metabolites-using-dual-emitting-zns-mn-2-quantum-dots-as-sole-luminophore-via-surface-chemistry-design
#11
Wenliang Gu, Suqin Gong, Yunlong Zhou, Yunsheng Xia
We herein present an effective and versatile platform for ratiometric sensing of metabolites using intrinsically dual-emitting ZnS:Mn(2+) quantum dots (QDs) as sole reporter. To avoid notoriously non-specific interactions, a special triple-layer "filter screen" around the inorganic QD core is rationally constructed, which is made of oleic acid, cetyltrimethyl ammonium bromide and bio-enzymes. In the presence of the analytes, the in-situ enzymatic H2O2 molecules diffuse and pass through the "filter screen" along the molecule interspace, which then reacts with the inorganic core and leads to more dramatically quenching of the Mn(2+) emission...
October 28, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27818052/scaffolds-for-oriented-and-close-packed-immobilization-of-immunoglobulins
#12
REVIEW
Masumi Iijima, Shun'ichi Kuroda
Immunosensing is a widely used technique that detects the interactions between antibodies and antigens such as biochemical markers, pathogens, allergens, and tumor-associated antigens. Since target antigens are often of high molecular mass and their binding affinities are sometimes weak, the spatial arrangement of immunoglobulin Gs (IgGs) on immunosensing probes should be optimized by presenting them in as close-packed a manner as possible and reducing steric hindrance around the antigen-binding Fv regions...
October 5, 2016: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/27812569/catalytic-promiscuity-of-glycopeptide-n-methyltransferases-enables-bio-orthogonal-labelling-of-biosynthetic-intermediates
#13
Clara Brieke, Grace Yim, Madeleine Peschke, Gerard D Wright, Max J Cryle
We show that two α-N-methyltransferases involved in the biosynthesis of glycopeptide antibiotics (GPAs) already recognise partly crosslinked precursor peptides of teicoplanin aglycone indicating that in vivo N-methylation can occur as an early tailoring step during GPA biosynthesis. This relaxed substrate specificity is accompanied by a remarkable promiscuity regarding the co-substrate enabling modulation of biological activity and the introduction of reactive handles which could be further modified using bio-orthogonal chemistry...
November 17, 2016: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/27785513/modulating-the-microtubule-tau-interactions-in-biomotility-systems-by-altering-the-chemical-environment
#14
S Bhattacharyya, K Kim, H Nakazawa, M Umetsu, W Teizer
Obstacles in microtubule mediated neuronal transport can trigger dementia. We use bio-motility assays, that simulate the neuron chemistry in axonopathy, to screen chemicals, that retain the microtubule dynamics in healthy neuronal activity. Tau protein inhibits microtubule activity and leads to oligomerization. Iron(iii) untangles, whereas mono-sodium-glutamate destabilizes the microtubule oligomer.
December 5, 2016: Integrative Biology: Quantitative Biosciences From Nano to Macro
https://www.readbyqxmd.com/read/27779846/hybrid-light-matter-states-in-a-molecular-and-material-science-perspective
#15
Thomas W Ebbesen
The notion that light and matter states can be hybridized the way s and p orbitals are mixed is a concept that is not familiar to most chemists and material scientists. Yet it has much potential for molecular and material sciences that is just beginning to be explored. For instance, it has already been demonstrated that the rate and yield of chemical reactions can be modified and that the conductivity of organic semiconductors and nonradiative energy transfer can be enhanced through the hybridization of electronic transitions...
October 25, 2016: Accounts of Chemical Research
https://www.readbyqxmd.com/read/27775329/smart-polymeric-hydrogels-for-cartilage-tissue-engineering-a-review-on-the-chemistry-and-biological-functions
#16
Niloofar Eslahi, Marjan Abdorahim, Abdolreza Arash Simchi
Stimuli responsive hydrogels (SRHs) are attractive bio-scaffolds for tissue engineering. The structural similarity of SRHs to the extracellular matrix (ECM) of many tissues offers great advantages for a minimally invasive tissue repair. Among various potential applications of SRHs, cartilage regeneration has attracted significant attention. The repair of cartilage damage is challenging in orthopedics owing to its low repair capacity. Recent advances include development of injectable hydrogels to minimize invasive surgery with nanostructured features and rapid stimuli-responsive characteristics...
October 24, 2016: Biomacromolecules
https://www.readbyqxmd.com/read/27775236/half-life-extension-of-biopharmaceuticals-using-chemical-methods-alternatives-to-pegylation
#17
REVIEW
Søren B van Witteloostuijn, Søren L Pedersen, Knud J Jensen
Peptides and proteins constitute a vast pool of excellent drug candidates. Evolution has equipped these molecules with superior drug-like properties such as high specificity and potency. However, native peptides and proteins suffer from an inadequate pharmacokinetic profile, and their outstanding pharmacological potential can only be realized if this issue is addressed during drug development. To overcome this challenge, a variety of half-life extension techniques relying on covalent chemical modification have been developed...
November 21, 2016: ChemMedChem
https://www.readbyqxmd.com/read/27767311/bio-inspired-multifunctional-membrane-for-aquatic-micro-pollutants-removal
#18
Xiaotong Cao, Jianquan Luo, John M Woodley, Yinhua Wan
Micro-pollutants present in water have many detrimental effects on the ecosystem. Membrane technology plays an important role in the removal of micro-pollutants but there remain significant challenges such as concentration polarization, membrane fouling and variable permeate quality. The work reported here uses a multifunctional membrane with rejection, adsorption and catalysis functions to solve these problems. Based on mussel-inspired chemistry and biological membrane properties, a multifunctional membrane was prepared by applying 'reverse filtration' of a laccase solution and subsequent 'dopamine coating' on a nanofiltration (NF) membrane support, which was tested on bisphenol A (BPA) removal...
October 21, 2016: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/27750460/cost-action-cm1201-biomimetic-radical-chemistry-free-radical-chemistry-successfully-meets-many-disciplines
#19
Carla Ferreri, Bernard T Golding, Ullrich Jahn, Jean-Luc Ravanat
The COST Action CM1201 "Biomimetic Radical Chemistry" has been active since December 2012 for four years, developing research topics organized into four working groups: WG1 - Radical Enzymes, WG2 - Models of DNA damage and consequences, WG3 - Membrane stress, signalling and defences, WG4 - Bio-inspired synthetic strategies. International collaborations have been established among the participating 80 research groups with brilliant interdisciplinary achievements. Free radical research with a biomimetic approach has been realized in the COST Action and are summarized in this overview by the four WG leaders...
October 17, 2016: Free Radical Research
https://www.readbyqxmd.com/read/27740717/luminescent-porous-polymers-based-on-aggregation-induced-mechanism-design-synthesis-and-functions
#20
Sasanka Dalapati, Cheng Gu, Donglin Jiang
Enormous research efforts are focusing on the design and synthesis of advanced luminescent systems, owing to their diverse capability in scientific studies and technological developments. In particular, fluorescence systems based on aggregation-induced emission (AIE) have emerged to show great potential for sensing, bio-imaging, and optoelectronic applications. Among them, integrating AIE mechanisms to design porous polymers is unique because it enables the combination of porosity and luminescence activity in one molecular skeleton for functional design...
October 14, 2016: Small
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