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Tissue Tracking

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https://www.readbyqxmd.com/read/29792882/utilization-of-automated-performance-metrics-to-measure-surgeon-performance-during-robotic-vesicourethral-anastomosis-and-methodical-development-of-a-training-tutorial
#1
Jian Chen, Paul J Oh, Nathan Cheng, Ankeet Shah, Jeremy Montez, Anthony Jarc, Liheng Guo, Inderbir S Gill, Andrew J Hung
PURPOSE: To develop and validate automated performance metrics (APMs) to measure surgeon performance of vesicourethral anastomosis (VUA) during robotic-assisted radical prostatectomy (RRP). Furthermore, we seek to methodically develop a standardized training tutorial for the robotic VUA. MATERIALS AND METHODS: APMs (motion tracking and system events data) and synchronized surgical video were captured during the RRP. Non-automated performance metrics (NAPMs) were manually annotated by video review...
May 21, 2018: Journal of Urology
https://www.readbyqxmd.com/read/29790493/quantum-dot-conjugated-nanobodies-for-multiplex-imaging-of-protein-dynamics-at-synapses
#2
Souvik Modi, Nathalie F Higgs, David Sheehan, Lewis D Griffin, Josef T Kittler
Neurons communicate with each other through synapses, which show enrichment for specialized receptors. Although many studies have explored spatial enrichment and diffusion of these receptors in dissociated neurons using single particle tracking, much less is known about their dynamic properties at synapses in complex tissue like brain slices. Here we report the use of smaller and highly specific quantum dots conjugated with a recombinant single domain antibody fragment (VHH fragment) against green fluorescent protein to provide information on diffusion of adhesion molecules at the growth cone and neurotransmitter receptors at synapses...
May 23, 2018: Nanoscale
https://www.readbyqxmd.com/read/29790424/the-fate-of-autologous-endometrial-mesenchymal-stromal-cells-after-application-in-the-healthy-equine-uterus
#3
Elisabeth Rink, Teresa Beyer, Hilari French, Elaine Watson, Christine Aurich, Xavier Donadeu
Because of their distinct differentiation, immunomodulatory and migratory capacities, endometrial mesenchymal stromal cells (MSCs) may provide an optimum source of therapeutic cells not only in relation to the uterus but also for regeneration of other tissues. This study reports the fate of endometrial MSCs following intrauterine application in mares. Stromal cell fractions were isolated from endometrial biopsies taken from seven reproductively healthy mares, expanded and fluorescence-labeled in culture. MSCs (15 x 106) or PBS were autologously infused into each uterine horn during early diestrus and subsequently tracked by fluorescence microscopy and flow cytometry of endometrial biopsies and blood samples taken periodically after infusion...
May 23, 2018: Stem Cells and Development
https://www.readbyqxmd.com/read/29789777/a-normalized-shear-deformation-indicator-for-ultrasound-strain-elastography-in-breast-tissues-an-in-vivo-feasibility-study
#4
Jingfeng Jiang, Bo Peng
The shear deformation under loads contains useful information for distinguishing benign breast lesions from malignant ones. In this study, we proposed a normalized shear deformation indicator (NSDI) that was derived from the concept of principal strains. Since the NSDI requires both high-quality axial and lateral (parallel and perpendicular to the beam, resp.) displacement estimates, a strategy combining high-quality speckle tracking with signal "denoising" was employed. Both techniques were previously published by our group...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29786554/a-human-ipsc-line-capable-of-differentiating-into-functional-macrophages-expressing-zsgreen-a-tool-for-the-study-and-in-vivo-tracking-of-therapeutic-cells
#5
Martha Lopez-Yrigoyen, Antonella Fidanza, Luca Cassetta, Richard A Axton, A Helen Taylor, Jose Meseguer-Ripolles, Anestis Tsakiridis, Valerie Wilson, David C Hay, Jeffrey W Pollard, Lesley M Forrester
We describe the production of a human induced pluripotent stem cell (iPSC) line, SFCi55-ZsGr, that has been engineered to express the fluorescent reporter gene, ZsGreen, in a constitutive manner. The CAG-driven ZsGreen expression cassette was inserted into the AAVS1 locus and a high level of expression was observed in undifferentiated iPSCs and in cell lineages derived from all three germ layers including haematopoietic cells, hepatocytes and neurons. We demonstrate efficient production of terminally differentiated macrophages from the SFCi55-ZsGreen iPSC line and show that they are indistinguishable from those generated from their parental SFCi55 iPSC line in terms of gene expression, cell surface marker expression and phagocytic activity...
July 5, 2018: Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
https://www.readbyqxmd.com/read/29780511/through-tissue-imaging-of-a-live-breast-cancer-tumour-model-using-handheld-surface-enhanced-spatially-offset-resonance-raman-spectroscopy-sesorrs
#6
Fay Nicolson, Lauren E Jamieson, Samuel Mabbott, Konstantinos Plakas, Neil C Shand, Michael R Detty, Duncan Graham, Karen Faulds
In order to improve patient survival and reduce the amount of unnecessary and traumatic biopsies, non-invasive detection of cancerous tumours is of imperative and urgent need. Multicellular tumour spheroids (MTS) can be used as an ex vivo cancer tumour model, to model in vivo nanoparticle (NP) uptake by the enhanced permeability and retention (EPR) effect. Surface enhanced spatially offset Raman spectroscopy (SESORS) combines both surface enhanced Raman spectroscopy (SERS) and spatially offset Raman spectroscopy (SORS) to yield enhanced Raman signals at much greater sub-surface levels...
April 21, 2018: Chemical Science
https://www.readbyqxmd.com/read/29774187/multimodality-imaging-for-the-quantitative-assessment-of-mitral-regurgitation
#7
REVIEW
Pei G Chew, Katrina Bounford, Sven Plein, Dominik Schlosshan, John P Greenwood
The natural history of mitral regurgitation (MR) results in significant morbidity and mortality. Innovations in non-invasive imaging have provided new insights into the pathophysiology and quantification of MR, in addition to early detection of left ventricular (LV) dysfunction and prognostic assessment in asymptomatic patients. Transthoracic (TTE) and transesophageal (TOE) echocardiography are the mainstay for diagnosis, assessment and serial surveillance. However, the advance from 2D to 3D imaging leads to improved assessment and characterization of mitral valve (MV) disease...
April 2018: Quantitative Imaging in Medicine and Surgery
https://www.readbyqxmd.com/read/29769030/in-silico-simulation-of-liver-crack-detection-using-ultrasonic-shear-wave-imaging
#8
Erwei Nie, Jiao Yu, Debaditya Dutta, Yanying Zhu
BACKGROUND: Liver trauma is an important source of morbidity and mortality worldwide. A timely detection and precise evaluation of traumatic liver injury and the bleeding site is necessary. There is a need to develop better imaging modalities of hepatic injuries to increase the sensitivity of ultrasonic imaging techniques for sites of hemorrhage caused by cracks. In this study, we conduct an in silico simulation of liver crack detection and delineation using an ultrasonic shear wave imaging (USWI) based method...
May 16, 2018: BMC Medical Imaging
https://www.readbyqxmd.com/read/29766272/blood-pressure-is-normal-but-is-the-heart
#9
Serkan Fazlı Çelik, Cemşit Karakurt, Yılmaz Tabel, Taner Elmas, Saim Yoloğlu
BACKGROUND: There is no detailed strain analysis of cardiac functions in treated hypertensive pediatric patients. The aim of this study was to evaluate the cardio-protective effects of different drug classes in treated pediatric hypertensive patients. METHODS: Sixty non-obese-treated hypertensive patients with preserved left ventricular (LV) systolic function and 45 age-, sex-, and body mass index-matched healthy subjects underwent clinical evaluation, including 24-h ambulatory blood pressure monitoring, standard echocardiographic examination, tissue Doppler imaging, and two-dimensional Speckle Tracking Echocardiography...
May 15, 2018: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
https://www.readbyqxmd.com/read/29763506/simplifying-zygomatic-implant-site-preparation-using-ultrasonic-navigation-a-technical-note
#10
Gerardo Pellegrino, Achille Tarsitano, Valerio Taraschi, Tomaso Vercellotti, Claudio Marchetti
Zygomatic implant site preparation could be considered a challenging procedure because of the use of a very long twist drill that could sometimes be difficult to control due to the need for drilling the malar bone on an oblique surface. Ultrasound was recently suggested to achieve better control, but the specific long tips required are not readily available, and the elongated tip also tends to reduce the efficacy. This technical note describes a proposal of a novel computer-aided technique to simplify the procedure of zygomatic implant site preparation...
May 2018: International Journal of Oral & Maxillofacial Implants
https://www.readbyqxmd.com/read/29760987/5-ala-fluorescence-and-laser-doppler-flowmetry-for-guidance-in-a-stereotactic-brain-tumor-biopsy
#11
Neda Haj-Hosseini, Johan C O Richter, Peter Milos, Martin Hallbeck, Karin Wårdell
A fiber optic probe was developed for guidance during stereotactic brain biopsy procedures to target tumor tissue and reduce the risk of hemorrhage. The probe was connected to a setup for the measurement of 5-aminolevulinic acid (5-ALA) induced fluorescence and microvascular blood flow. Along three stereotactic trajectories, fluorescence (n = 109) and laser Doppler flowmetry (LDF) (n = 144) measurements were done in millimeter increments. The recorded signals were compared to histopathology and radiology images...
May 1, 2018: Biomedical Optics Express
https://www.readbyqxmd.com/read/29759948/measuring-relative-positions-and-orientations-of-the-tibia-with-respect-to-the-femur-using-one-channel-3d-tracked-a-mode-ultrasound-tracking-system-a-cadaveric-study
#12
Kenan Niu, Jasper Homminga, Victor Sluiter, André Sprengers, Nico Verdonschot
The purpose of this study is to investigate the technical feasibility of measuring relative positions and orientations of the tibia with respect to the femur in an in-vitro experiment by using a 3D-tracked A-mode ultrasound system and to determine its accuracy of angular and translational measurements. As A-mode ultrasound is capable of detecting bone surface through soft tissue in a non-invasive manner, the combination of a single A-mode ultrasound transducer with an optical motion tracking system provides the possibility for digitizing the 3D locations of bony points at different anatomical regions on the thigh and the shank...
May 11, 2018: Medical Engineering & Physics
https://www.readbyqxmd.com/read/29755444/microfluidic-pcr-amplification-and-miseq-amplicon-sequencing-techniques-for-high-throughput-detection-and-genotyping-of-human-pathogenic-rna-viruses-in-human-feces-sewage-and-oysters
#13
Mamoru Oshiki, Takayuki Miura, Shinobu Kazama, Takahiro Segawa, Satoshi Ishii, Masashi Hatamoto, Takashi Yamaguchi, Kengo Kubota, Akinori Iguchi, Tadashi Tagawa, Tsutomu Okubo, Shigeki Uemura, Hideki Harada, Naohiro Kobayashi, Nobuo Araki, Daisuke Sano
Detection and genotyping of pathogenic RNA viruses in human and environmental samples are useful for monitoring the circulation and prevalence of these pathogens, whereas a conventional PCR assay followed by Sanger sequencing is time-consuming and laborious. The present study aimed to develop a high-throughput detection-and-genotyping tool for 11 human RNA viruses [Aichi virus; astrovirus; enterovirus; norovirus genogroup I (GI), GII, and GIV; hepatitis A virus; hepatitis E virus; rotavirus; sapovirus; and human parechovirus] using a microfluidic device and next-generation sequencer...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29754780/single-cell-transcriptomics-meets-lineage-tracing
#14
REVIEW
Lennart Kester, Alexander van Oudenaarden
Reconstructing lineage relationships between cells within a tissue or organism is a long-standing aim in biology. Traditionally, lineage tracing has been achieved through the (genetic) labeling of a cell followed by the tracking of its offspring. Currently, lineage trajectories can also be predicted using single-cell transcriptomics. Although single-cell transcriptomics provides detailed phenotypic information, the predicted lineage trajectories do not necessarily reflect genetic relationships. Recently, techniques have been developed that unite these strategies...
May 3, 2018: Cell Stem Cell
https://www.readbyqxmd.com/read/29754007/magnetic-particle-imaging-for-radiation-free-sensitive-and-high-contrast-vascular-imaging-and-cell-tracking
#15
REVIEW
Xinyi Y Zhou, Zhi Wei Tay, Prashant Chandrasekharan, Elaine Y Yu, Daniel W Hensley, Ryan Orendorff, Kenneth E Jeffris, David Mai, Bo Zheng, Patrick W Goodwill, Steven M Conolly
Magnetic particle imaging (MPI) is an emerging ionizing radiation-free biomedical tracer imaging technique that directly images the intense magnetization of superparamagnetic iron oxide nanoparticles (SPIOs). MPI offers ideal image contrast because MPI shows zero signal from background tissues. Moreover, there is zero attenuation of the signal with depth in tissue, allowing for imaging deep inside the body quantitatively at any location. Recent work has demonstrated the potential of MPI for robust, sensitive vascular imaging and cell tracking with high contrast and dose-limited sensitivity comparable to nuclear medicine...
May 10, 2018: Current Opinion in Chemical Biology
https://www.readbyqxmd.com/read/29752051/effect-of-shoulder-model-complexity-in-upper-body-kinematics-analysis-of-the-golf-swing
#16
M Bourgain, S Hybois, P Thoreux, O Rouillon, P Rouch, C Sauret
The golf swing is a complex full body movement during which the spine and shoulders are highly involved. In order to determine shoulder kinematics during this movement, multibody kinematics optimization (MKO) can be recommended to limit the effect of the soft tissue artifact and to avoid joint dislocations or bone penetration in reconstructed kinematics. Classically, in golf biomechanics research, the shoulder is represented by a 3 degrees-of-freedom model representing the glenohumeral joint. More complex and physiological models are already provided in the scientific literature...
April 25, 2018: Journal of Biomechanics
https://www.readbyqxmd.com/read/29751558/connexin-communication-compartments-and-wound-repair-in-epithelial-tissue
#17
REVIEW
Marc Chanson, Masakatsu Watanabe, Erin M O'Shaughnessy, Alice Zoso, Patricia E Martin
Epithelial tissues line the lumen of tracts and ducts connecting to the external environment. They are critical in forming an interface between the internal and external environment and, following assault from environmental factors and pathogens, they must rapidly repair to maintain cellular homeostasis. These tissue networks, that range from a single cell layer, such as in airway epithelium, to highly stratified and differentiated epithelial surfaces, such as the epidermis, are held together by a junctional nexus of proteins including adherens, tight and gap junctions, often forming unique and localised communication compartments activated for localised tissue repair...
May 3, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29751141/tracking-translocation-of-self-discriminating-curcumin-hybrid-nanocrystals-following-intravenous-delivery
#18
Ting Wang, Jianping Qi, Ning Ding, Xiaochun Dong, Weili Zhao, Yi Lu, Changhong Wang, Wei Wu
Nanocrystals hold great potential as parenteral delivery carrier systems for poorly water-soluble drugs. Elucidation of the in vivo fate of parenteral nanocrystals is of pharmacological, toxicological and mechanistic significance. However, it is of tremendous difficulty to monitor real-time translocation of nanocrystals in vivo owing to progressive dissolution of nanocrystals and a lack of workable tools to probe nanocrystals. In this study, self-discriminating hybrid nanocrystals (SDHNs) of a model drug curcumin (CUR) were developed by embedding traces of environment-responsive fluorescent dyes into the crystalline lattices of CUR...
May 8, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29750152/automatic-myotendinous-junction-tracking-in-ultrasound-images-with-phase-based-segmentation
#19
Guang-Quan Zhou, Yi Zhang, Ruo-Li Wang, Ping Zhou, Yong-Ping Zheng, Olga Tarassova, Anton Arndt, Qiang Chen
Displacement of the myotendinous junction (MTJ) obtained by ultrasound imaging is crucial to quantify the interactive length changes of muscles and tendons for understanding the mechanics and pathological conditions of the muscle-tendon unit during motion. However, the lack of a reliable automatic measurement method restricts its application in human motion analysis. This paper presents an automated measurement of MTJ displacement using prior knowledge on tendinous tissues and MTJ, precluding the influence of nontendinous components on the estimation of MTJ displacement...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29749652/evolution-of-echocardiography-in-subclinical-detection-of-cancer-therapy-related-cardiac-dysfunction
#20
REVIEW
Rohit Moudgil, Saamir Hassan, Nicolas Palaskas, Juan Lopez-Mattei, Jose Banchs, Syed Wamique Yusuf
Cancer therapies have resulted in increased survivorship in oncological patients. However, the benefits have been marred by the development of premature cardiovascular disease. The current definition outlines measurement of ejection fraction as a mean to diagnose cancer therapeutic-related cardiac dysfunction (CTRCD); however, up to 58% of the patients do not regain their cardiac function after the CTRCD diagnosis, despite therapeutic interventions. Therefore, there has been a growing interest in the markers for early myocardial changes (ie, changes with normal left ventricular ejection fraction [LVEF]) that may predict the development of subsequent left ventricular ejection fraction reduction or progression to heart failure...
May 11, 2018: Echocardiography
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