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3D Bioprinting

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https://www.readbyqxmd.com/read/28524375/bioprinted-osteogenic-and-vasculogenic-patterns-for-engineering-3d-bone-tissue
#1
Batzaya Byambaa, Nasim Annabi, Kan Yue, Grissel Trujillo-de Santiago, Mario Moisés Alvarez, Weitao Jia, Mehdi Kazemzadeh-Narbat, Su Ryon Shin, Ali Tamayol, Ali Khademhosseini
Fabricating 3D large-scale bone tissue constructs with functional vasculature has been a particular challenge in engineering tissues suitable for repairing large bone defects. To address this challenge, an extrusion-based direct-writing bioprinting strategy is utilized to fabricate microstructured bone-like tissue constructs containing a perfusable vascular lumen. The bioprinted constructs are used as biomimetic in vitro matrices to co-culture human umbilical vein endothelial cells and bone marrow derived human mesenchymal stem cells in a naturally derived hydrogel...
May 19, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28520521/three-dimensional-cell-cultures-in-drug-discovery-and-development
#2
Ye Fang, Richard M Eglen
The past decades have witnessed significant efforts toward the development of three-dimensional (3D) cell cultures as systems that better mimic in vivo physiology. Today, 3D cell cultures are emerging, not only as a new tool in early drug discovery but also as potential therapeutics to treat disease. In this review, we assess leading 3D cell culture technologies and their impact on drug discovery, including spheroids, organoids, scaffolds, hydrogels, organs-on-chips, and 3D bioprinting. We also discuss the implementation of these technologies in compound identification, screening, and development, ranging from disease modeling to assessment of efficacy and safety profiles...
June 2017: SLAS Discovery
https://www.readbyqxmd.com/read/28512850/in-situ-handheld-3d-bioprinting-for-cartilage-regeneration
#3
Claudia Di Bella, Serena Duchi, Cathal D O'Connell, Romane Blanchard, Cheryl Augustine, Zhilian Yue, Fletcher Thompson, Christopher Richards, Stephen Beirne, Carmine Onofrillo, Sebastien H Bauquier, Stewart D Ryan, Peter Pivonka, Gordon G Wallace, Peter F Choong
Articular cartilage injuries experienced at an early age can lead to the development of osteoarthritis later in life. In situ 3D printing is an exciting and innovative bio-fabrication technology that enables the surgeon to deliver tissue- engineering techniques at the time and location of need. We have created a hand- held 3D printing device (Biopen) that allows the simultaneous co-axial extrusion of bioscaffold and cultured cells directly into the cartilage defect in vivo in a single session surgery. This pilot study assesses the ability of the Biopen to repair a full thickness chondral defect and the early outcomes in cartilage regeneration, and compares these results to other treatments in a large animal model...
May 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28508071/self-assembled-micro-organogels-for-3d-printing-silicone-structures
#4
Christopher S O'Bryan, Tapomoy Bhattacharjee, Samuel Hart, Christopher P Kabb, Kyle D Schulze, Indrasena Chilakala, Brent S Sumerlin, W Gregory Sawyer, Thomas E Angelini
The widespread prevalence of commercial products made from microgels illustrates the immense practical value of harnessing the jamming transition; there are countless ways to use soft, solid materials that fluidize and become solid again with small variations in applied stress. The traditional routes of microgel synthesis produce materials that predominantly swell in aqueous solvents or, less often, in aggressive organic solvents, constraining ways that these exceptionally useful materials can be used. For example, aqueous microgels have been used as the foundation of three-dimensional (3D) bioprinting applications, yet the incompatibility of available microgels with nonpolar liquids, such as oils, limits their use in 3D printing with oil-based materials, such as silicone...
May 2017: Science Advances
https://www.readbyqxmd.com/read/28501712/3d-bioprinting-for-drug-discovery-and-development-in-pharmaceutics
#5
REVIEW
Weijie Peng, Pallab Datta, Bugra Ayan, Veli Ozbolat, Donna Sosnoski, Ibrahim T Ozbolat
Successful launch of a commercial drug requires significant investment of time and financial resources wherein late-stage failures become a reason for catastrophic failures in drug discovery. This calls for infusing constant innovations in technologies, which can give reliable prediction of efficacy, and more importantly, toxicology of the compound early in the drug discovery process before clinical trials. Though computational advances have resulted in more rationale in silico designing, in vitro experimental studies still require gaining industry confidence and improving in vitro-in vivo correlations...
May 10, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28469183/coaxial-3d-bioprinting-of-self-assembled-multicellular-heterogeneous-tumor-fibers
#6
Xingliang Dai, Libiao Liu, Jia Ouyang, Xinda Li, Xinzhi Zhang, Qing Lan, Tao Xu
Three-dimensional (3D) bioprinting of living structures with cell-laden biomaterials has been achieved in vitro, however, some cell-cell interactions are limited by the existing hydrogel. To better mimic tumor microenvironment, self-assembled multicellular heterogeneous brain tumor fibers have been fabricated by a custom-made coaxial extrusion 3D bioprinting system, with high viability, proliferative activity and efficient tumor-stromal interactions. Therein, in order to further verify the sufficient interactions between tumor cells and stroma MSCs, CRE-LOXP switch gene system which contained GSCs transfected with "LOXP-STOP-LOXP-RFP" genes and MSCs transfected with "CRE recombinase" gene was used...
May 3, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28467835/self-supporting-nanoclay-as-internal-scaffold-material-for-direct-printing-of-soft-hydrogel-composite-structures-in-air
#7
Yifei Jin, Chengcheng Liu, Wenxuan Chai, Ashley Compaan, Yong Huang
Three dimensional (3D) bioprinting technology enables the freeform fabrication of complex constructs from various hydrogels and is receiving increasing attention in tissue engineering. The objective of this study is to develop a novel self-supporting direct hydrogel printing approach to extrude complex 3D hydrogel composite structures in air without the help of a support bath. Laponite, a member of the smectite mineral family, is investigated to serve as an internal scaffold material for the direct printing of hydrogel composite structures in air...
May 11, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28464555/extrusion-bioprinting-of-shear-thinning-gelatin-methacryloyl-bioinks
#8
Wanjun Liu, Marcel A Heinrich, Yixiao Zhou, Ali Akpek, Ning Hu, Xiao Liu, Xiaofei Guan, Zhe Zhong, Xiangyu Jin, Ali Khademhosseini, Yu Shrike Zhang
Bioprinting is an emerging technique for the fabrication of 3D cell-laden constructs. However, the progress for generating a 3D complex physiological microenvironment has been hampered by a lack of advanced cell-responsive bioinks that enable bioprinting with high structural fidelity, particularly in the case of extrusion-based bioprinting. Herein, this paper reports a novel strategy to directly bioprint cell-laden gelatin methacryloyl (GelMA) constructs using bioinks of GelMA physical gels (GPGs) achieved through a simple cooling process...
May 2, 2017: Advanced Healthcare Materials
https://www.readbyqxmd.com/read/28458963/is-3d-bioprinting-the-future-of-reconstructive-surgery
#9
Nicolas Sigaux, Léa Pourchet, Marion Albouy, Amélie Thépot, Christophe Marquette
No abstract text is available yet for this article.
March 2017: Plastic and Reconstructive Surgery. Global Open
https://www.readbyqxmd.com/read/28457175/tissue-engineering-and-regenerative-medicine-new-trends-and-directions-a-year-in-review
#10
Manuela E Gomes, Márcia T Rodrigues, Rui M A Domingues, Rui L Reis
Tissue Engineering (TE) is continuously evolving assimilating inputs from adjacent scientific areas and their technological advances, including nanotechnology developments that have been spawning the range of available options for the precise manipulation and control of cells and cellular environments. Simultaneously, with the maturation of the field, TE is having a growing and marked impact in other fields, such as cancer and other diseases research, enabling tri-dimensional (3D) tumor/tissue models of increased complexity that more closely resemble living tissue dynamics, playing a decisive role in the development of new and improved therapies...
April 29, 2017: Tissue Engineering. Part B, Reviews
https://www.readbyqxmd.com/read/28453913/bioprinting-of-biomimetic-skin-containing-melanocytes
#11
Daejin Min, Wonhye Lee, Il-Hong Bae, Tae Ryong Lee, Phillip Croce, Seung-Schik Yoo
This study reports a three-dimensional (3D) bioprinting technique that is capable of producing a full-thickness skin model containing pigmentation. Multiple layers of fibroblasts (FB)-containing collagen hydrogel precursor were printed and crosslinked through neutralization using sodium bicarbonate, constituting the dermal layer. Melanocytes (MC) and keratinocytes (KC) were sequentially printed on top of the dermal layer to induce skin pigmentation upon subsequent air-liquid interface culture. Histological analysis was performed not only to confirm the formation of distinct skin layers, but also to identify the presence of pigmentation...
April 28, 2017: Experimental Dermatology
https://www.readbyqxmd.com/read/28433241/mechanics-of-additively-manufactured-biomaterials
#12
EDITORIAL
Amir A Zadpoor
Additive manufacturing (3D printing) has found many applications in healthcare including fabrication of biomaterials as well as bioprinting of tissues and organs. Additively manufactured (AM) biomaterials may possess arbitrarily complex micro-architectures that give rise to novel mechanical, physical, and biological properties. The mechanical behavior of such porous biomaterials including their quasi-static mechanical properties and fatigue resistance is not yet well understood. It is particularly important to understand the relationship between the designed micro-architecture (topology) and the resulting mechanical properties...
June 2017: Journal of the Mechanical Behavior of Biomedical Materials
https://www.readbyqxmd.com/read/28429072/kidney-development-and-perspectives-for-organ-engineering
#13
REVIEW
Ganna Reint, Aleksandra Rak-Raszewska, Seppo J Vainio
Organ transplantation is currently the best strategy for treating end stage renal disease (ESRD) but the numbers of donor kidneys available are not sufficient to meet the needs of the ever-increasing ESRD population. Therefore, developments in the field of tissue engineering are necessary to provide alternative treatments. Decellularization and three-dimensional (3D) bioprinting strategies may serve as attractive novel options. Since successful tissue engineering requires an in -depth understanding of organ development and regulatory pathways, we discuss signaling in renal development and the composition of the renal extracellular matrix before presenting progress in the decellularization and 3D bioprinting fields...
April 20, 2017: Cell and Tissue Research
https://www.readbyqxmd.com/read/28424770/recent-advances-in-bioink-design-for-3d-bioprinting-of-tissues-and-organs
#14
REVIEW
Shen Ji, Murat Guvendiren
There is a growing demand for alternative fabrication approaches to develop tissues and organs as conventional techniques are not capable of fabricating constructs with required structural, mechanical, and biological complexity. 3D bioprinting offers great potential to fabricate highly complex constructs with precise control of structure, mechanics, and biological matter [i.e., cells and extracellular matrix (ECM) components]. 3D bioprinting is an additive manufacturing approach that utilizes a "bioink" to fabricate devices and scaffolds in a layer-by-layer manner...
2017: Frontiers in Bioengineering and Biotechnology
https://www.readbyqxmd.com/read/28416222/dentin-on-the-nanoscale-hierarchical-organization-mechanical-behavior-and-bioinspired-engineering
#15
REVIEW
Luiz E Bertassoni
OBJECTIVE: Knowledge of the structural organization and mechanical properties of dentin has expanded considerably during the past two decades, especially on a nanometer scale. In this paper, we review the recent literature on the nanostructural and nanomechanical properties of dentin, with special emphasis in its hierarchical organization. METHODS: We give particular attention to the recent literature concerning the structural and mechanical influence of collagen intrafibrillar and extrafibrillar mineral in healthy and remineralized tissues...
April 14, 2017: Dental Materials: Official Publication of the Academy of Dental Materials
https://www.readbyqxmd.com/read/28388499/microfluidic-enhanced-3d-bioprinting-of-aligned-myoblast-laden-hydrogels-leads-to-functionally-organized-myofibers-in%C3%A2-vitro-and-in%C3%A2-vivo
#16
Marco Costantini, Stefano Testa, Pamela Mozetic, Andrea Barbetta, Claudia Fuoco, Ersilia Fornetti, Francesco Tamiro, Sergio Bernardini, Jakub Jaroszewicz, Wojciech Święszkowski, Marcella Trombetta, Luisa Castagnoli, Dror Seliktar, Piotr Garstecki, Gianni Cesareni, Stefano Cannata, Alberto Rainer, Cesare Gargioli
We present a new strategy for the fabrication of artificial skeletal muscle tissue with functional morphologies based on an innovative 3D bioprinting approach. The methodology is based on a microfluidic printing head coupled to a co-axial needle extruder for high-resolution 3D bioprinting of hydrogel fibers laden with muscle precursor cells (C2C12). To promote myogenic differentiation, we formulated a tailored bioink with a photocurable semi-synthetic biopolymer (PEG-Fibrinogen) encapsulating cells into 3D constructs composed of aligned hydrogel fibers...
July 2017: Biomaterials
https://www.readbyqxmd.com/read/28386058/cartilage-tissue-engineering-by-the-3d-bioprinting-of-ips-cells-in-a-nanocellulose-alginate-bioink
#17
Duong Nguyen, Daniel A Hägg, Alma Forsman, Josefine Ekholm, Puwapong Nimkingratana, Camilla Brantsing, Theodoros Kalogeropoulos, Samantha Zaunz, Sebastian Concaro, Mats Brittberg, Anders Lindahl, Paul Gatenholm, Annika Enejder, Stina Simonsson
Cartilage lesions can progress into secondary osteoarthritis and cause severe clinical problems in numerous patients. As a prospective treatment of such lesions, human-derived induced pluripotent stem cells (iPSCs) were shown to be 3D bioprinted into cartilage mimics using a nanofibrillated cellulose (NFC) composite bioink when co-printed with irradiated human chondrocytes. Two bioinks were investigated: NFC with alginate (NFC/A) or hyaluronic acid (NFC/HA). Low proliferation and phenotypic changes away from pluripotency were seen in the case of NFC/HA...
April 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28351222/bone-regenerative-medicine-in-oral-and-maxillofacial-region-using-a-3d-printer
#18
Atsuhiko Hikita, Ung-Il Chung, Kazuto Hoshi, Tsuyoshi Takato
Bone grafts currently used for the treatment of large bone defect or asymmetry in oral and maxillofacial region include autologous, allogenic and artificial bones. Although artificial bone is free from the concerns of donor site morbidity, limitation of volume, disease transmission and ethical issues, it lacks osteogenic and osteoinductive activities. In addition, molding of the artificial bone is an issue especially when it is used for the augmentation of bone as onlay grafts. To solve this problem, additive manufacturing techniques have been applied to fabricate bones which have outer shapes conformed to patients' bones...
March 29, 2017: Tissue Engineering. Part A
https://www.readbyqxmd.com/read/28337147/3d-proximal-tubule-tissues-recapitulate-key-aspects-of-renal-physiology-to-enable-nephrotoxicity-testing
#19
Shelby M King, J William Higgins, Celina R Nino, Timothy R Smith, Elizabeth H Paffenroth, Casey E Fairbairn, Abigail Docuyanan, Vishal D Shah, Alice E Chen, Sharon C Presnell, Deborah G Nguyen
Due to its exposure to high concentrations of xenobiotics, the kidney proximal tubule is a primary site of nephrotoxicity and resulting attrition in the drug development pipeline. Current pre-clinical methods using 2D cell cultures and animal models are unable to fully recapitulate clinical drug responses due to limited in vitro functional lifespan, or species-specific differences. Using Organovo's proprietary 3D bioprinting platform, we have developed a fully cellular human in vitro model of the proximal tubule interstitial interface comprising renal fibroblasts, endothelial cells, and primary human renal proximal tubule epithelial cells to enable more accurate prediction of tissue-level clinical outcomes...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28333087/magnetically-bioprinted-human-myometrial-3d-cell-rings-as-a-model-for-uterine-contractility
#20
Glauco R Souza, Hubert Tseng, Jacob A Gage, Arunmani Mani, Pujan Desai, Fransisca Leonard, Angela Liao, Monica Longo, Jerrie S Refuerzo, Biana Godin
Deregulation in uterine contractility can cause common pathological disorders of the female reproductive system, including preterm labor, infertility, inappropriate implantation, and irregular menstrual cycle. A better understanding of human myometrium contractility is essential to designing and testing interventions for these important clinical problems. Robust studies on the physiology of human uterine contractions require in vitro models, utilizing a human source. Importantly, uterine contractility is a three-dimensionally (3D)-coordinated phenomenon and should be studied in a 3D environment...
March 23, 2017: International Journal of Molecular Sciences
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