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3D Bioprinting

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https://www.readbyqxmd.com/read/29656237/vascularized-microfluidic-organ-chips-for-drug-screening-disease-models-and-tissue-engineering
#1
REVIEW
Tatsuya Osaki, Vivek Sivathanu, Roger D Kamm
Vascularization of micro-tissues in vitro has enabled formation of tissues larger than those limited by diffusion with appropriate nutrient/gas exchange as well as waste elimination. Furthermore, angiocrine signaling from the vasculature may be essential in mimicking organ-level functions in these micro-tissues. In drug screening applications, the presence of an appropriate blood-organ barrier in the form of a vasculature and its supporting cells (pericytes, appropriate stromal cells) may be essential to reproducing organ-scale drug delivery pharmacokinetics...
April 12, 2018: Current Opinion in Biotechnology
https://www.readbyqxmd.com/read/29652626/endosteal-and-perivascular-subniches-in-a-3d-bone-marrow-model-for-multiple-myeloma
#2
Maaike Vera Jasmijn Braham, Tilman Ahlfeld, Ashwini Rahul Akkineni, Monique C Minnema, Wouter Dhert, F Cumhur Oner, Catherine Robin, Anja Lode, Michael Gelinsky, Jacqueline Alblas
The bone marrow microenvironment is the preferred location of multiple myeloma, supporting tumor growth and development. It is composed of a collection of interacting subniches, including the endosteal and perivascular niche. Current in vitro models mimic either of these subniches. By developing a model combining both niches, this study aims to further enhance the ability to culture primary myeloma cells in vitro. Also the dependency of myeloma cells on each niche was studied. A 3D bone marrow model containing two subniches was created using 3D bioprinting technology...
March 8, 2018: Tissue Engineering. Part C, Methods
https://www.readbyqxmd.com/read/29642573/3d-printing-of-thermo-responsive-methylcellulose-hydrogels-for-cell-sheet-engineering
#3
Andrea Cochis, Lorenzo Bonetti, Rita Sorrentino, Nicola Contessi Negrini, Federico Grassi, Massimiliano Leigheb, Lia Rimondini, Silvia Farè
A possible strategy in regenerative medicine is cell-sheet engineering (CSE), i.e., developing smart cell culture surfaces from which to obtain intact cell sheets (CS). The main goal of this study was to develop 3D printing via extrusion-based bioprinting of methylcellulose (MC)-based hydrogels. Hydrogels were prepared by mixing MC powder in saline solutions (Na₂SO₄ and PBS). MC-based hydrogels were analyzed to investigate the rheological behavior and thus optimize the printing process parameters. Cells were tested in vitro on ring-shaped printed hydrogels; bulk MC hydrogels were used for comparison...
April 10, 2018: Materials
https://www.readbyqxmd.com/read/29623827/comparative-assay-of-2d-and-3d-cell-culture-models-proliferation-gene-expression-and-anticancer-drug-response
#4
Aline G Souza, Isaura Beatriz B Silva, Esther Campos-Fernandez, Leticia S Barcelos, Jessica Brito Souza, Karina Marangoni, Luiz R Goulart, Vivian Alonso-Goulart
In vitro tests allow establishing experimental variables. However, in vitro results cannot be extrapolated to in vivo tests. Considering that three-dimensional (3D) culture has been one of the best ways to portray the in vivo system of most cell types, it is possible to carry out assays with a great clinical relevance for the analysis of the screening, action and resistance of antitumor drugs. Thus, the objective of the present study was to compare between 2D and 3D cell culture forms to conclude which is the most suitable model for preclinical in vitro drug testing...
April 4, 2018: Current Pharmaceutical Design
https://www.readbyqxmd.com/read/29594704/bioprinted-gelatin-hydrogel-platform-promotes-smooth-muscle-cell-contractile-phenotype-maintenance
#5
Ajay Tijore, Jean-Marc Behr, Scott Alexander Irvine, Vrushali Baisane, Subbu Venkatraman
Three dimensional (3D) bioprinting has been proposed as a method for fabricating tissue engineered small diameter vascular prostheses. This technique not only involves constructing the structural features to obtain a desired pattern but the morphology of the pattern may also be used to influence the behavior of seeded cells. Herein, we 3D bioprinted a gelatin hydrogel microchannel construct to promote and preserve the contractile phenotype of vascular smooth muscle cells (vSMCs), which is crucial for vasoresponsiveness...
March 28, 2018: Biomedical Microdevices
https://www.readbyqxmd.com/read/29593958/3d-bioprinted-osteoblast-laden-nanocomposite-hydrogel-constructs-with-induced-microenvironments-promote-cell-viability-differentiation-and-osteogenesis-both-in-vitro-and-in-vivo
#6
Xinyun Zhai, Changshun Ruan, Yufei Ma, Delin Cheng, Mingming Wu, Wenguang Liu, Xiaoli Zhao, Haobo Pan, William Weijia Lu
An osteoblast-laden nanocomposite hydrogel construct, based on polyethylene glycol diacrylate (PEGDA)/laponite XLG nanoclay ([Mg5.34 Li0.66 Si8 O20 (OH)4 ]Na0.66, clay )/hyaluronic acid sodium salt (HA) bio-inks, is developed by a two-channel 3D bioprinting method. The novel biodegradable bio-ink A, comprised of a poly(ethylene glycol) (PEG)-clay nanocomposite crosslinked hydrogel, is used to facilitate 3D-bioprinting and enables the efficient delivery of oxygen and nutrients to growing cells. HA with encapsulated primary rat osteoblasts (ROBs) is applied as bio-ink B with a view to improving cell viability, distribution uniformity, and deposition efficiency...
March 2018: Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
https://www.readbyqxmd.com/read/29578448/laser-bioprinting-of-human-induced-pluripotent-stem-cells-the-effect-of-printing-and-biomaterials-on-cell-survival-pluripotency-and-differentiation
#7
Lothar Koch, Andrea Deiwick, Annika Franke, Kristin Schwanke, Axel Haverich, Robert Zweigerdt, Boris N Chichkov
Research on human induced pluripotent stem cells (hiPSCs) is one of the fastest growing fields in biomedicine. Generated from patient's own somatic cells, hiPSCs can be differentiated towards all functional cell types and returned to the patient without immunological concerns. 3D printing of hiPSCs could enable generation of functional organs for replacement therapies or realization of organ-on-chip systems for individualized medicine. 
 Printing of living cells was demonstrated with immortalized cell lines, primary cells, and adult stem cells with different printing technologies and biomaterials...
March 26, 2018: Biofabrication
https://www.readbyqxmd.com/read/29570458/3d-printing-strategies-for-peripheral-nerve-regeneration
#8
Eugen B Petcu, Rajiv Midha, Erin McColl, Aurel Popa-Wagner, Traian V Chirila, Paul D Dalton
After many decades of biomaterials research for peripheral nerve regeneration, a clinical product (the nerve guide), is emerging as a proven alternative for relatively short injury gaps. This review identifies aspects where 3D printing can assist in improving long-distance nerve guide regeneration strategies. These include (1) 3D printing of the customizable nerve guides, (2) fabrication of scaffolds that fill nerve guides, (3) 3D bioprinting of cells within a matrix/bioink into the nerve guide lumen and the (4) establishment of growth factor gradients along the length a nerve guide...
March 23, 2018: Biofabrication
https://www.readbyqxmd.com/read/29565814/bioprinting-perfusion-enabled-liver-equivalents-for-advanced-organ-on-a-chip-applications
#9
Tobias Grix, Alicia Ruppelt, Alexander Thomas, Anna-Klara Amler, Benjamin P Noichl, Roland Lauster, Lutz Kloke
Many tissue models have been developed to mimic liver-specific functions for metabolic and toxin conversion in in vitro assays. Most models represent a 2D environment rather than a complex 3D structure similar to native tissue. To overcome this issue, spheroid cultures have become the gold standard in tissue engineering. Unfortunately, spheroids are limited in size due to diffusion barriers in their dense structures, limiting nutrient and oxygen supply. Recent developments in bioprinting techniques have enabled us to engineer complex 3D structures with perfusion-enabled channel systems to ensure nutritional supply within larger, densely-populated tissue models...
March 22, 2018: Genes
https://www.readbyqxmd.com/read/29559838/decellularized-hydrogels-in-bone-tissue-engineering-a-topical-review
#10
REVIEW
Andrea Pacifici, Luigi Laino, Marco Gargari, Federico Guzzo, Andrea Velandia Luz, Antonella Polimeni, Luciano Pacifici
Nowadays, autograft and allograft techniques represent the main solution to improve bone repair. Unfortunately, autograft technique is expensive, invasive and subject to infections and hematoma, frequently affecting both donor sites and surgical sites. A recent advance in tissue engineering is the fabrication of cell-laden hydrogels with custom-made geometry, depending on the clinical case. The use of ECM (Extra-Cellular Matrix)-derived Hydrogels from bone tissue is the new opportunity to obtain good results in bone regeneration...
2018: International Journal of Medical Sciences
https://www.readbyqxmd.com/read/29558424/3d-printability-of-alginate-carboxymethyl-cellulose-hydrogel
#11
Ahasan Habib, Venkatachalem Sathish, Sanku Mallik, Bashir Khoda
Three-dimensional (3D) bio-printing is a revolutionary technology to reproduce a 3D functional living tissue scaffold in-vitro through controlled layer-by-layer deposition of biomaterials along with high precision positioning of cells. Due to its bio-compatibility, natural hydrogels are commonly considered as the scaffold material. However, the mechanical integrity of a hydrogel material, especially in 3D scaffold architecture, is an issue. In this research, a novel hybrid hydrogel, that is, sodium alginate with carboxymethyl cellulose (CMC) is developed and systematic quantitative characterization tests are conducted to validate its printability, shape fidelity and cell viability...
March 20, 2018: Materials
https://www.readbyqxmd.com/read/29555462/effects-of-tunable-3d-bioprinted-hydrogels-on-human-brown-adipocyte-behavior-and-metabolic-function
#12
Mitchell Kuss, Jiyoung Kim, Dianjun Qi, Shaohua Wu, Yuguo Lei, Soonkyu Chung, Bin Duan
Obesity and its related health complications cause billions of dollars in healthcare costs annually in the United States, and there are yet to be safe and long-lasting anti-obesity approaches. Using brown adipose tissue (BAT) is a promising approach, as it uses fats for energy expenditure. However, the effect of the microenvironment on human thermogenic brown adipogenesis and how to generate clinically relevant sized and functioning BAT are still unknown. In our current study, we evaluated the effects of endothelial growth medium exposure on brown adipogenesis of human brown adipose progenitors (BAP)...
March 16, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29550442/3d-freeform-printing-of-silk-fibroin
#13
Maria J Rodriguez, Thomas A Dixon, Eliad Cohen, Wenwen Huang, Fiorenzo G Omenetto, David L Kaplan
Freeform fabrication has emerged as a key direction in printing biologically-relevant materials and structures. With this emerging technology, complex structures with microscale resolution can be created in arbitrary geometries and without the limitations found in traditional bottom-up or top-down additive manufacturing methods. Recent advances in freeform printing have used the physical properties of microparticle-based granular gels as a medium for the submerged extrusion of bioinks. However, most of these techniques require post-processing or crosslinking for the removal of the printed structures [1,2]...
March 14, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29535776/translational-application-of-microfluidics-and-bioprinting-for-stem-cell-based-cartilage-repair
#14
REVIEW
Silvia Lopa, Carlotta Mondadori, Valerio Luca Mainardi, Giuseppe Talò, Marco Costantini, Christian Candrian, Wojciech Święszkowski, Matteo Moretti
Cartilage defects can impair the most elementary daily activities and, if not properly treated, can lead to the complete loss of articular function. The limitations of standard treatments for cartilage repair have triggered the development of stem cell-based therapies. In this scenario, the development of efficient cell differentiation protocols and the design of proper biomaterial-based supports to deliver cells to the injury site need to be addressed through basic and applied research to fully exploit the potential of stem cells...
2018: Stem Cells International
https://www.readbyqxmd.com/read/29517901/three-dimensional-bioprinting-of-oppositely-charged-hydrogels-with-super-strong-interface-bonding
#15
Huijun Li, Yu Jun Tan, Sijun Liu, Lin Li
A novel strategy to improve the adhesion between printed layers of 3D printed constructs is developed by exploiting the interaction between two oppositely charged hydrogels. Three anionic hydrogels (alginate, xanthan, kappa-carrageenan (Kca)) and three cationic hydrogels (chitosan, gelatin, gelatin methacrylate (GelMA)) are chosen in order to find the optimal combination of two oppositely charged hydrogels for the best 3D printability with strong interface bonding. Rheological properties and printability of the hydrogels, as well as structural integrity of printed constructs in cell culture medium, are studied as functions of polymer concentration and combination of hydrogels...
March 8, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29510003/a-bilayer-photoreceptor-retinal-tissue-model-with-gradient-cell-density-design-a-study-of-microvalve-based-bioprinting
#16
Pujiang Shi, Tan Yong Sheng Edgar, Wai Yee Yeong, Li Hoi Yeung, Augustinus Laude
ARPE-19 and Y79 cells were precisely and effectively delivered to form an in vitro retinal tissue model via 3D cell bioprinting technology. The samples were characterized by cell viability assay, hematoxylin and eosin (HE) and immunofluorescent staining, scanning electrical microscopy (SEM) and confocal microscopy etc. The bioprinted ARPE-19 cells formed a high-quality cell monolayer in 14 days. Manually seeded ARPE-19 cells were poorly controlled during and after cell seeding, and they aggregated to form uneven cell layer...
March 6, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29507817/engineering-3d-approaches-to-model-the-dynamic-microenvironments-of-cancer-bone-metastasis
#17
REVIEW
Han Qiao, Tingting Tang
Cancer metastasis to bone is a three-dimensional (3D), multistep, dynamic process that requires the sequential involvement of three microenvironments, namely, the primary tumour microenvironment, the circulation microenvironment and the bone microenvironment. Engineered 3D approaches allow for a vivid recapitulation of in vivo cancerous microenvironments in vitro, in which the biological behaviours of cancer cells can be assessed under different metastatic conditions. Therefore, modelling bone metastasis microenvironments with 3D cultures is imperative for advancing cancer research and anti-cancer treatment strategies...
2018: Bone Research
https://www.readbyqxmd.com/read/29492503/bioinks-for-3d-bioprinting-an-overview
#18
REVIEW
P Selcan Gungor-Ozkerim, Ilyas Inci, Yu Shrike Zhang, Ali Khademhosseini, Mehmet Remzi Dokmeci
Bioprinting is an emerging technology with various applications in making functional tissue constructs to replace injured or diseased tissues. It is a relatively new approach that provides high reproducibility and precise control over the fabricated constructs in an automated manner, potentially enabling high-throughput production. During the bioprinting process, a solution of a biomaterial or a mixture of several biomaterials in the hydrogel form, usually encapsulating the desired cell types, termed the bioink, is used for creating tissue constructs...
March 1, 2018: Biomaterials Science
https://www.readbyqxmd.com/read/29490802/tedd-annual-meeting-with-3d-bioprinting-workshop
#19
Michael Raghunath, Markus Rimann, Katarzyna S Kopanska, Sandra Laternser
Bioprinting is the technology of choice for realizing functional tissues such as vascular system, muscle, cartilage and bone. In the future, bioprinting will influence the way we engineer tissues and bring it to a new level of physiological relevance. That was the topic of the 2017 TEDD Annual Meeting at ZHAW Waedenswil on 8th and 9th November. In an exciting workshop, the two companies regenHU Ltd. and CELLINK gave us an insight into highly topical applications and collaborations in this domain.
February 1, 2018: Chimia
https://www.readbyqxmd.com/read/29489338/potential-and-limitations-of-nanocelluloses-as-components-in-biocomposite-inks-for-three-dimensional-bioprinting-and-for-biomedical-devices
#20
Gary Chinga-Carrasco
Three-dimensional (3D) printing has rapidly emerged as a new technology with a wide range of applications that includes biomedicine. Some common 3D printing methods are based on the suitability of biopolymers to be extruded through a nozzle to construct a 3D structure layer by layer. Nanocelluloses with specific rheological characteristics are suitable components to form inks for 3D printing. This review considers various nanocelluloses that have been proposed for 3D printing with a focus on the potential advantages, limitations, and requirements when used for biomedical devices and when used in contact with the human body...
February 28, 2018: Biomacromolecules
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