Michail Shapira, Alexandra Dobysh, Anastasia Liaudanskaya, Hanna Aucharova, Yaraslau Dzichenka, Volha Bokuts, Suzana Jovanović-Šanta, Aliaksey Yantsevich
Cholesterol oxidases (ChOxes) are enzymes that catalyze the oxidation of cholesterol to cholest-4-en-3-one. These enzymes find wide applications across various diagnostic and industrial settings. In addition, as a pathogenic factor of several bacteria, they have significant clinical implications. The current classification system for ChOxes is based on the type of bond connecting FAD to the apoenzyme, which does not adequately illustrate the enzymatic and structural characteristics of these proteins. In this study, we have adopted an integrative approach, combining evolutionary analysis, classic enzymatic techniques and computational approaches, to elucidate the distinct features of four various ChOxes from Rhodococcussp...
December 15, 2023: Biochimie