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https://www.readbyqxmd.com/read/28599463/cancer-gene-panel-analysis-of-cultured-circulating-tumor-cells-and-primary-tumor-tissue-from-patients-with-breast-cancer
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Eunjoo Hwang, Ji-Hyun Uh, Hye Seon Lee, Cham Han Lee, Soo Jeong Lee, Sei Hyun Ahn, Byung Ho Son, Jong Won Lee, Jong Han Yu, Nak-Jung Kwon, Woo Chung Lee, Kap-Seok Yang, Sung Ho Choi, Myoung Shin Kim, Jinseon Lee, Byung Hee Jeon
Although numerous effective therapies have improved the survival rate of patients with breast cancer, a number of patients present with tumor recurrence and metastasis. A liquid biopsy of circulating tumor cells (CTC) is a non-invasive method to obtain tumor cells and may be used as substitute for a tumor tissue biopsy. The present study focuses on determining whether CTC culture is an optimal method of obtaining sufficient amounts of CTCs for molecular analysis. The current study demonstrates a method of isolating and culturing CTCs from patients with breast cancer and the construction of a molecular profile of cultured cells using the Ion AmpliSeq Cancer Gene Panel V2...
June 2017: Oncology Letters
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