keyword
MENU ▼
Read by QxMD icon Read
search

Gate analysis

keyword
https://www.readbyqxmd.com/read/28642681/%C3%AE-1-containing-gaba-a-receptors-cluster-at-synapses-where-they-mediate-slower-synaptic-currents-than-%C3%AE-2-containing-gaba-a-receptors
#1
Christine L Dixon, Pankaj Sah, Angelo Keramidas, Joseph W Lynch, Nela Durisic
GABA-A receptors (GABAARs) are pentameric ligand-gated ion channels that are assembled mainly from α (α1-6), β (β1-3) and γ (γ1-3) subunits. Although GABAARs containing γ2L subunits mediate most of the inhibitory neurotransmission in the brain, significant expression of γ1 subunits is seen in the amygdala, pallidum and substantia nigra. However, the location and function of γ1-containing GABAARs in these regions remains unclear. In "artificial" synapses, where the subunit composition of postsynaptic receptors is specifically controlled, γ1 incorporation slows the synaptic current decay rate without affecting channel deactivation, suggesting that γ1-containing receptors are not clustered and therefore activated by diffuse neurotransmitter...
2017: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/28642232/a-single-amino-acid-residue-at-transmembrane-domain-4-of-the-alpha-subunit-influences-carisoprodol-direct-gating-efficacy-at-gabaa-receptors
#2
Manoj Kumar, Manish Kumar, John Freund, Glenn H Dillon
The muscle relaxant carisoprodol (CSP, trade name Soma) has recently been controlled at the federal level as a Schedule IV drug due to its high abuse potential and consequences of misuse, such as withdrawal syndrome, delusions, seizures and even death. Recent work has shown that carisoprodol can directly gate and allosterically modulate the GABAA receptor. These actions are subunit-dependent; compared to other GABAA receptors, carisoprodol has nominal direct gating effects in α3β2γ2receptors. Here, using site-directed-mutagenesis and whole cell patch clamp electrophysiology in transiently transfected HEK293 cells, we examined the role of GABAA receptor α subunit transmembrane domain 4 (TM4) amino acids in direct gating and allosteric modulatory actions of carisoprodol...
June 22, 2017: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/28640577/laser-ablation-molecular-isotopic-spectrometry-for-molecules-formation-chemistry-in-fs-laser-ablated-plasmas
#3
Huaming Hou, Xianglei Mao, Vassilia Zorba, Richard E Russo
Recently, laser ablated molecular isotopic spectrometry (LAMIS) has expanded its capability to explore molecules formation mechanism in laser induced plasma in addition to isotope analysis. LAMIS is powerful tool for tracking the origination of atoms that involved in formation of investigated molecules by labeling atoms with its isotopic substitution. The evolutionary formation pathways of organic molecules, especially of C2 dimers and CN radicals, were frequently reported. However, very little is known about the formation pathways for metallic radicals and heterodimers in laser ablated plasma...
June 22, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28639400/motion-estimation-for-cardiac-functional-analysis-using-two-x-ray-computed-tomography-scans
#4
George S K Fung, Luisa Ciuffo, Hiroshi Ashikaga, Katsuyuki Taguchi
PURPOSE: This work concerns computed tomography (CT)-based cardiac functional analysis (CFA) with a reduced radiation dose. As CT-CFA requires images over the entire heartbeat, the scans are often performed at 10-20% of the tube current settings that are typically used for coronary CT angiography. A large image noise then degrades the accuracy of motion estimation. Moreover, even if the scan was performed during the sinus rhythm, the cardiac motion observed in CT images may not be cyclic with patients with atrial fibrillation...
June 22, 2017: Medical Physics
https://www.readbyqxmd.com/read/28638135/temperature-dependence-of-protein-water-interactions-in-a-gated-yeast-aquaporin
#5
Camilo Aponte-Santamaría, Gerhard Fischer, Petra Båth, Richard Neutze, Bert L de Groot
Regulation of aquaporins is a key process of living organisms to counteract sudden osmotic changes. Aqy1, which is a water transporting aquaporin of the yeast Pichia pastoris, is suggested to be gated by chemo-mechanical stimuli as a protective regulatory-response against rapid freezing. Here, we tested the influence of temperature by determining the X-ray structure of Aqy1 at room temperature (RT) at 1.3 Å resolution, and by exploring the structural dynamics of Aqy1 during freezing through molecular dynamics simulations...
June 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28636915/probing-the-structural-dynamics-of-the-nmda-receptor-activation-by-coarse-grained-modeling
#6
Wenjun Zheng, Han Wen, Gary J Iacobucci, Gabriela K Popescu
N-Methyl-D-aspartate (NMDA) receptors are glutamate-gated excitatory channels that play essential roles in brain functions. High-resolution structures have been solved for an allosterically inhibited and agonist-bound form of a functional NMDA receptor; however, other key functional states (particularly the active open-channel state) were only resolved at moderate resolutions by cryo-electron microscopy (cryo-EM). To decrypt the mechanism of the NMDA receptor activation, structural modeling is essential to provide presently missing information about structural dynamics...
June 20, 2017: Biophysical Journal
https://www.readbyqxmd.com/read/28635673/toxicity-of-naphthalene-and-benzene-on-tribollium-castaneum-herbst
#7
Nerlis Pajaro-Castro, Karina Caballero-Gallardo, Jesus Olivero-Verbel
Naphthalene and benzene are widely-used volatile organic compounds. The aim of this research was to examine the toxicological effects of naphthalene and benzene against Tribolium castaneum as an animal model. Adult insects were exposed to these aromatic compounds to assess mortality after 4-48 h of exposure. The lethal concentration 50 (LC50) for naphthalene, naphthalin, and benzene were 63.6 µL/L, 20.0 µL/L, and 115.9 µL/L in air, respectively. Real-time polymerase chain reaction (PCR) analysis revealed expression changes in genes related to oxidative stress and metabolism [Glutathione S-Transferase (Gst), and Cytochrome P450 6BQ8 (Cyp6bq8)]; reproduction and metamorphosis [Hormone receptor in 39-like protein (Hr39), Ecdysone receptor: (Ecr), and Chitin synthase 2 (Chs2)]; and neurotransmission [Histamine-gated chloride channel 2 (Hiscl2)] in insects exposed for 4 h to 70...
June 21, 2017: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/28634442/mild-traumatic-brain-injury-evokes-pyramidal-neuron-axon-initial-segment-plasticity-and-diffuse-presynaptic-inhibitory-terminal-loss
#8
Michal Vascak, Jianli Sun, Matthew Baer, Kimberle M Jacobs, John T Povlishock
The axon initial segment (AIS) is the site of action potential (AP) initiation, thus a crucial regulator of neuronal activity. In excitatory pyramidal neurons, the high density of voltage-gated sodium channels (NaV1.6) at the distal AIS regulates AP initiation. A surrogate AIS marker, ankyrin-G (ankG) is a structural protein regulating neuronal functional via clustering voltage-gated ion channels. In neuronal circuits, changes in presynaptic input can alter postsynaptic output via AIS structural-functional plasticity...
2017: Frontiers in Cellular Neuroscience
https://www.readbyqxmd.com/read/28631080/comparison-of-volumetric-and-functional-parameters-in-simultaneous-cardiac-pet-mr-feasibility-of-volumetric-assessment-with-residual-activity-from-prior-pet-ct
#9
C Lücke, B Oppolzer, P Werner, B Foldyna, P Lurz, T Jochimsen, B Brenneis, L Lehmkuhl, B Sattler, M Grothoff, H Barthel, O Sabri, M Gutberlet
OBJECTIVE: To compare cardiac left ventricular (LV) parameters in simultaneously acquired hybrid fluorine-18-fluorodeoxyglucose ([18F] FDG) positron emission tomography/magnetic resonance imaging (PET/MRI) in patients with residual tracer activity of upstream PET/CT. METHODS: Twenty-nine patients (23 men, age 58±17 years) underwent cardiac PET/MRI either directly after a non-cardiac PET/CT with homogenous cardiac [18F] FDG uptake (n=20) or for viability assessment (n=9)...
June 19, 2017: European Radiology
https://www.readbyqxmd.com/read/28630044/single-molecule-fret-analysis-discloses-the-dynamics-of-the-dna-topoisomerase-ii-top2-interaction-in-the-presence-of-top2-targeting-agents
#10
Wan-Chen Huang, Chun-Ying Lee, Tao-Shih Hsieh
Topoisomerases play crucial roles in DNA replication, transcription, and recombination. For instance, topoisomerase II (Top2) is critically important for resolving DNA tangles during cell division, and as such, it is a broad anticancer drug target. Top2 regulates DNA topology by transiently breaking one double stranded DNA molecule (cleavage), allowing a second double strand to pass through the opened DNA gate (opening), and then closing the gate by rejoining the broken ends. Drugs that modulate Top2 catalysis may therefore affect enzymatic activity at several different steps...
June 19, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28628372/suicide-intervention-training-for-college-staff-program-evaluation-and-intervention-skill-measurement
#11
Laura Shannonhouse, Yung-Wei Dennis Lin, Kelly Shaw, Reema Wanna, Michael Porter
OBJECTIVE: Suicide remains a pressing issue for college communities. Consequently, gate-keeper trainings are often provided for staff. This study examines the effect of one such program, Applied Suicide Intervention Skills Training (ASIST). PARTICIPANTS: 51 college employees received ASIST in August of 2014 and were compared to 30 wait-list control participants. METHODS: MR-ANOVA were used to analyze pre- and post-training: (a) skills at responding to students-at-risk; (b) attitudes towards suicide; (c) knowledge about suicide; and (d) comfort/competence/confidence at helping a student-at-risk...
June 19, 2017: Journal of American College Health: J of ACH
https://www.readbyqxmd.com/read/28625793/comparison-of-sputum-collection-methods-for-tuberculosis-diagnosis-a-systematic-review-and-pairwise-and-network-meta-analysis
#12
Sumona Datta, Lena Shah, Robert H Gilman, Carlton A Evans
BACKGROUND: The performance of laboratory tests to diagnose pulmonary tuberculosis is dependent on the quality of the sputum sample tested. The relative merits of sputum collection methods to improve tuberculosis diagnosis are poorly characterised. We therefore aimed to investigate the effects of sputum collection methods on tuberculosis diagnosis. METHODS: We did a systematic review and meta-analysis to investigate whether non-invasive sputum collection methods in people aged at least 12 years improve the diagnostic performance of laboratory testing for pulmonary tuberculosis...
June 15, 2017: Lancet Global Health
https://www.readbyqxmd.com/read/28623170/nicotinic-acetylcholine-receptor-inhibitors-derived-from-snake-and-snail-venoms
#13
REVIEW
Sebastien Dutertre, Annette Nicke, Victor Tsetlin
The nicotinic acetylcholine receptor (nAChR) represents the prototype of ligand-gated ion channels. It is vital for neuromuscular transmission and an important regulator of neurotransmission. A variety of toxic compounds derived from diverse species target this receptor and have been of elemental importance in basic and applied research. They enabled milestone discoveries in pharmacology and biochemistry ranging from the original formulation of the receptor concept, the first isolation and structural analysis of a receptor protein (the nAChR) to the identification, localization, and differentiation of its diverse subtypes and their validation as a target for therapeutic intervention...
June 13, 2017: Neuropharmacology
https://www.readbyqxmd.com/read/28621350/photoinduced-structural-distortions-and-singlet-triplet-intersystem-crossing-in-cu-i-mlct-excited-states-monitored-by-optically-gated-fluorescence-spectroscopy
#14
Sofia Garakyaraghi, Petr Koutnik, Felix N Castellano
Copper(i) phenanthroline complexes represent viable earth-abundant alternatives to the ubiquitous Ru(ii) tris-bipyridine photosensitizers owing to their similar metal-to-ligand charge transfer (MLCT) properties. A well-established complication of Cu(i) phenanthroline complexes is that they can undergo significant photo-induced structural rearrangements, leading to excited states that are highly susceptible to exciplex formation and short-lived. In this work, a comprehensive analysis of the photo-induced structural distortions and singlet-triplet intersystem crossing dynamics of a series of four sterically encumbered Cu(i) phenanthroline chromophores has been conducted, namely, [Cu(dsbp)2](+) (dsbp = 2,9-di-sec-butyl-1,10-phenanthroline), [Cu(dsbtmp)2](+) (dsbtmp = 2,9-di-sec-butyl-3,4,7,8-tetramethyl-1,10-phenanthroline), [Cu(dipp)2](+) (dipp = 2,9-di-isopropyl-1,10-phenanthroline), and [Cu(diptmp)2](+) (diptmp = 2,9-di-isopropyl-3,4,7,8-tetramethyl-1,10-phenanthroline)...
June 16, 2017: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/28620319/vascular-endothelial-growth-factor-b-induces-a-distinct-electrophysiological-phenotype-in-mouse-heart
#15
Nikolay Naumenko, Jenni Huusko, Tomi Tuomainen, Jussi T Koivumäki, Mari Merentie, Erika Gurzeler, Kari Alitalo, Riikka Kivelä, Seppo Ylä-Herttuala, Pasi Tavi
Vascular endothelial growth factor B (VEGF-B) is a potent mediator of vascular, metabolic, growth, and stress responses in the heart, but the effects on cardiac muscle and cardiomyocyte function are not known. The purpose of this study was to assess the effects of VEGF-B on the energy metabolism, contractile, and electrophysiological properties of mouse cardiac muscle and cardiac muscle cells. In vivo and ex vivo analysis of cardiac-specific VEGF-B TG mice indicated that the contractile function of the TG hearts was normal...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28619229/cost-effectiveness-of-xpert-mtb-rif-for-tuberculosis-diagnosis-in-south-africa-a-real-world-cost-analysis-and-economic-evaluation
#16
Anna Vassall, Mariana Siapka, Nicola Foster, Lucy Cunnama, Lebogang Ramma, Katherine Fielding, Kerrigan McCarthy, Gavin Churchyard, Alison Grant, Edina Sinanovic
BACKGROUND: In 2010 a new diagnostic test for tuberculosis, Xpert MTB/RIF, received a conditional programmatic recommendation from WHO. Several model-based economic evaluations predicted that Xpert would be cost-effective across sub-Saharan Africa. We investigated the cost-effectiveness of Xpert in the real world during national roll-out in South Africa. METHODS: For this real-world cost analysis and economic evaluation, we applied extensive primary cost and patient event data from the XTEND study, a pragmatic trial examining Xpert introduction for people investigated for tuberculosis in 40 primary health facilities (20 clusters) in South Africa enrolled between June 8, and Nov 16, 2012, to estimate the costs and cost per disability-adjusted life-year averted of introducing Xpert as the initial diagnostic test for tuberculosis, compared with sputum smear microscopy (the standard of care)...
July 2017: Lancet Global Health
https://www.readbyqxmd.com/read/28619228/effect-of-community-health-clubs-on-child-diarrhoea-in-western-rwanda-cluster-randomised-controlled-trial
#17
Sheela S Sinharoy, Wolf-Peter Schmidt, Ronald Wendt, Leodomir Mfura, Erin Crossett, Karen A Grépin, William Jack, Bernard Ngabo Rwabufigiri, James Habyarimana, Thomas Clasen
BACKGROUND: Community health clubs are multi-session village-level gatherings led by trained facilitators and designed to promote healthy behaviours mainly related to water, sanitation, and hygiene. They have been implemented in several African and Asian countries but have never been evaluated rigorously. We aimed to evaluate the effect of two versions of the community health club model on child health and nutrition outcomes. METHODS: We did a cluster-randomised trial in Rusizi district, western Rwanda...
July 2017: Lancet Global Health
https://www.readbyqxmd.com/read/28618603/analytical-solution-of-the-steady-membrane-voltage-fluctuation-caused-by-a-single-ion-channel
#18
Jia-Zeng Wang, Rui-Zhen Wang
An analytical steady-state solution of the stochastic model describing the integrated dynamics of the membrane voltage and the gating of a single channel is presented. The voltage density function experiences bifurcation in the parameter space, and the threshold is determined by the comparison between the rates of the voltage evolution and that of the channel gating. It is singular at the reversal potential if the voltage evolves faster than the channel gating. As an application, the entropy production rate associated with the gating currents is calculated; its variation tendency in parameter space is consistent to that of the number of transitions counted from the sample paths...
May 2017: Physical Review. E
https://www.readbyqxmd.com/read/28618453/data-driven-programmatic-approach-to-analysis-of-basophil-activation-tests
#19
Sarita U Patil, Augustin Calatroni, Michael Schneider, Johanna Steinbrecher, Neal Smith, Cecilia Washburn, Alex Ma, Wayne G Shreffler
BACKGROUND: Conventional data analysis of flow cytometry-based basophil activation testing requires repetitive, labor-intensive analysis that hampers efforts to standardize testing for clinical applications. Using an open-source platform, we developed and implemented a programmatic approach to the analysis of the basophil activation test (BAT) by flow cytometry. METHODS: Using the BÜHLMANN FlowCAST® assay, peripheral blood from peanut allergic patients undergoing oral immunotherapy was incubated with peanut allergens (Arah1, Arah2, Arah6, whole peanut extract) and stained with fluorescent antibodies to CCR3 and CD63 for the development of a data-driven programmatic analysis using Bioconductor and R...
June 15, 2017: Cytometry. Part B, Clinical Cytometry
https://www.readbyqxmd.com/read/28618451/multicenter-comparison-of-cd34-myeloid-cell-count-by-flow-cytometry-in-low-risk-myelodysplastic-syndrome-is-it-feasible
#20
Patricia Font, Dolores Subirá, Sergio Matarraz, Celina Benavente, María Teresa Cedena, Marta Morado, Ana Pérez Corral, José María Bellón, José Luis Díez-Martín
BACKGROUND: Accuracy of bone marrow (BM) blast count in low-risk myelodysplastic syndromes (MDS) still remains a challenge though it is essential for prognosis. We investigated whether the enumeration of CD34+ myeloid cells by flow cytometry immunophenotyping (FCI) could be used as a consistent parameter for clinical MDS studies. METHODS: Six clinical centres entered the study and information on their FCI protocols was recorded. Sixty-seven flow cytometry listmodes from BM samples of patients with low-risk MDS with <5% BM blasts were exchanged among participants in two different rounds...
June 15, 2017: Cytometry. Part B, Clinical Cytometry
keyword
keyword
78235
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"