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https://www.readbyqxmd.com/read/29784951/tomographic-and-multimodal-scattering-type-scanning-near-field-optical-microscopy-with-peak-force-tapping-mode
#1
Haomin Wang, Le Wang, Devon S Jakob, Xiaoji G Xu
Scattering-type scanning near-field optical microscopy (s-SNOM) enables nanoscale spectroscopic imaging and has been instrumental for many nano-photonic discoveries and in situ studies. However, conventional s-SNOM techniques with atomic force microscopy tapping mode operation and lock-in detections do not provide direct tomographic information with explicit tip-sample distance. Here, we present a non-traditional s-SNOM technique, named peak force scattering-type scanning near-field optical microscopy (PF-SNOM), by combination of peak force tapping mode and time-gated light detection...
May 21, 2018: Nature Communications
https://www.readbyqxmd.com/read/29784819/xenoprotein-engineering-via-synthetic-libraries
#2
Zachary P Gates, Alexander A Vinogradov, Anthony J Quartararo, Anupam Bandyopadhyay, Zi-Ning Choo, Ethan D Evans, Kathryn H Halloran, Alexander J Mijalis, Surin K Mong, Mark D Simon, Eric A Standley, Evan D Styduhar, Sarah Z Tasker, Faycal Touti, Jessica M Weber, Jessica L Wilson, Timothy F Jamison, Bradley L Pentelute
Chemical methods have enabled the total synthesis of protein molecules of ever-increasing size and complexity. However, methods to engineer synthetic proteins comprising noncanonical amino acids have not kept pace, even though this capability would be a distinct advantage of the total synthesis approach to protein science. In this work, we report a platform for protein engineering based on the screening of synthetic one-bead one-compound protein libraries. Screening throughput approaching that of cell surface display was achieved by a combination of magnetic bead enrichment, flow cytometry analysis of on-bead screens, and high-throughput MS/MS-based sequencing of identified active compounds...
May 21, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29783982/providing-japanese-health-care-information-for-international-visitors-digital-animation-intervention
#3
Mariko Nishikawa, Masaaki Yamanaka, Junko Kiriya, Masamine Jimba
BACKGROUND: Over 24 million international visitors came to Japan in 2016 and the number is expected to increase. Visitors could be at a risk of illness or injury that may result in hospitalization in Japan. We assessed the effects of a four-minute digital animation titled Mari Info Japan on the level of anxiety experienced by international visitors to Japan. METHODS: We conducted a non-randomized, controlled study at Narita International Airport outside Tokyo in December 2014...
May 21, 2018: BMC Health Services Research
https://www.readbyqxmd.com/read/29782667/synergies-of-electrochemical-metallization-and-valance-change-in-all-inorganic-perovskite-quantum-dots-for-resistive-switching
#4
Yan Wang, Ziyu Lv, Qiufan Liao, Haiquan Shan, Jinrui Chen, Ye Zhou, Li Zhou, Xiaoli Chen, Vellaisamy A L Roy, Zhanpeng Wang, Zongxiang Xu, Yu-Jia Zeng, Su-Ting Han
The in-depth understanding of ions' generation and movement inside all-inorganic perovskite quantum dots (CsPbBr3 QDs), which may lead to a paradigm to break through the conventional von Neumann bottleneck, is strictly limited. Here, it is shown that formation and annihilation of metal conductive filaments and Br- ion vacancy filaments driven by an external electric field and light irradiation can lead to pronounced resistive-switching effects. Verified by field-emission scanning electron microscopy as well as energy-dispersive X-ray spectroscopy analysis, the resistive switching behavior of CsPbBr3 QD-based photonic resistive random-access memory (RRAM) is initiated by the electrochemical metallization and valance change...
May 21, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29780811/does-size-matter-to-models-exploring-the-effect-of-herd-size-on-outputs-of-a-herd-level-disease-spread-simulator
#5
Mary Van Andel, Tracey Hollings, Richard Bradhurst, Andrew Robinson, Mark Burgman, M Carolyn Gates, Paul Bingham, Tim Carpenter
Disease spread modeling is widely used by veterinary authorities to predict the impact of emergency animal disease outbreaks in livestock and to evaluate the cost-effectiveness of different management interventions. Such models require knowledge of basic disease epidemiology as well as information about the population of animals at risk. Essential demographic information includes the production system, animal numbers, and their spatial locations yet many countries with significant livestock industries do not have publically available and accurate animal population information at the farm level that can be used in these models...
2018: Frontiers in Veterinary Science
https://www.readbyqxmd.com/read/29779223/gene-editing-vectors-for-studying-nicotinic-acetylcholine-receptors-in-cholinergic-transmission
#6
Can Peng, Yijin Yan, Veronica J Kim, Staci E Engle, Jennifer N Berry, J Michael McIntosh, Rachael L Neve, Ryan M Drenan
Nicotinic acetylcholine receptors (nAChRs), prototype members of the cys-loop ligand gated ion channel family, are key mediators of cholinergic transmission in the central nervous system. Despite their importance, technical gaps exist in our ability to dissect the function of individual subunits in the brain. To overcome these barriers, we designed CRISPR/Cas9 small guide RNA sequences (sgRNAs) for production of loss-of-function alleles in mouse nAChR genes. These sgRNAs were validated in vitro via deep sequencing...
May 19, 2018: European Journal of Neuroscience
https://www.readbyqxmd.com/read/29770356/nonvolatile-infrared-memory-in-mos-2-pbs-van-der-waals-heterostructures
#7
Qisheng Wang, Yao Wen, Kaiming Cai, Ruiqing Cheng, Lei Yin, Yu Zhang, Jie Li, Zhenxing Wang, Feng Wang, Fengmei Wang, Tofik Ahmed Shifa, Chao Jiang, Hyunsoo Yang, Jun He
Optoelectronic devices for information storage and processing are at the heart of optical communication technology due to their significant applications in optical recording and computing. The infrared radiations of 850, 1310, and 1550 nm with low energy dissipation in optical fibers are typical optical communication wavebands. However, optoelectronic devices that could convert and store the infrared data into electrical signals, thereby enabling optical data communications, have not yet been realized. We report an infrared memory device using MoS2 /PbS van der Waals heterostructures, in which the infrared pulse intrigues a persistent resistance state that hardly relaxes within our experimental time scales (more than 104 s)...
April 2018: Science Advances
https://www.readbyqxmd.com/read/29767241/transcriptome-sequencing-analysis-reveals-the-effect-of-combinative-treatment-with-low%C3%A2-intensity-pulsed-ultrasound-and-magnesium-ions-on-hfob1-19-human-osteoblast-cells
#8
Haiyue Zu, Xueting Yi, Dewei Zhao
Biodegradable magnesium (Mg) materials are considered ideal as osteosynthesis implants. However, clinical application has proven complex. This is primarily associated with the issue of reducing the extent of implant degradation to a range acceptable for the human body, while simultaneously enhancing osteogenesis or osteoinduction. In the present study, a combination of Mg ions and low‑intensity pulsed ultrasound (LIPUS) treatment was applied in hFOB 1.19 human osteoblast cells as a potential strategy to resolve this issue...
May 11, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29761986/-a-capillary-blood-flow-velocity-detection-system-based-on-linear-array-charge-coupled-devices
#9
Houming Zhou, Ruofeng Wang, Qi Dang, Li Yang, Xiang Wang
In order to detect the flow characteristics of blood samples in the capillary, this paper introduces a blood flow velocity measurement system based on field-programmable gate array (FPGA), linear charge-coupled devices (CCD) and personal computer (PC) software structure. Based on the analysis of the TCD1703C and AD9826 device data sheets, Verilog HDL hardware description language was used to design and simulate the driver. Image signal acquisition and the extraction of the real-time edge information of the blood sample were carried out synchronously in the FPGA...
December 1, 2017: Sheng Wu Yi Xue Gong Cheng Xue za Zhi, Journal of Biomedical Engineering, Shengwu Yixue Gongchengxue Zazhi
https://www.readbyqxmd.com/read/29760086/mapping-the-functional-anatomy-of-orai1-transmembrane-domains-for-crac-channel-gating
#10
Priscilla S-W Yeung, Megumi Yamashita, Christopher E Ing, Régis Pomès, Douglas M Freymann, Murali Prakriya
Store-operated Orai1 channels are activated through a unique inside-out mechanism involving binding of the endoplasmic reticulum Ca2+ sensor STIM1 to cytoplasmic sites on Orai1. Although atomic-level details of Orai structure, including the pore and putative ligand binding domains, are resolved, how the gating signal is communicated to the pore and opens the gate is unknown. To address this issue, we used scanning mutagenesis to identify 15 residues in transmembrane domains (TMs) 1-4 whose perturbation activates Orai1 channels independently of STIM1...
May 14, 2018: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/29758532/evaluation-of-the-nova-vet-meter-for-sheep-side-monitoring-of-%C3%AE-hydroxybutyric-acid-bhba-and-description-of-ewe-bhba-during-late-gestation-in-three-flocks-from-the-northeastern-u-s
#11
Amanda K Jones, Rachael E Gately, Tasia D Kellogg, Steven A Zinn, Kristen E Govoni, Sarah A Reed
Prevention of metabolic diseases in small ruminants may improve production efficiency and profitability, yet ewes carrying multiples or who are in poor body condition are at increased susceptibility to develop ketosis. This study evaluated the hand-held Nova Vet Meter to accurately detect β-hydroxybutyric acid (BHBA) concentrations in ewes and determined the percentage of ewes at moderate (0.8 to 1.5 mmol/L BHBA) and greatest (≥1.6 mmol/L BHBA) risk to develop ketosis during late gestation. To validate the Nova Vet Meter, BHBA concentrations of 104 paired blood samples were measured using the Nova Vet Meter and gold-standard laboratory analysis...
May 7, 2018: Research in Veterinary Science
https://www.readbyqxmd.com/read/29753948/gender-related-differences-observed-among-immune-cells-in-synovial-fluid-in-knee-osteoarthritis
#12
E Kriegova, G Manukyan, Z Mikulkova, G Gabcova, M Kudelka, P Gajdos, J Gallo
OBJECTIVE: There is no existing comprehensive report on the cellular composition of synovial fluids (SFs) from knee osteoarthritis (OA). We therefore aimed to characterise the immune cell composition in SFs from knee OA and in subgroups according to gender. DESIGN: The immunophenotyping of monocyte/macrophage lineage cells, T and B cells, NK cells, neutrophils, dendritic and mast cells present in SFs from 53 patients (24 males/29 females) with knee OA was performed using 6-colour flow cytometry...
May 10, 2018: Osteoarthritis and Cartilage
https://www.readbyqxmd.com/read/29753354/the-development-of-the-guide-to-economic-analysis-and-research-gear-online-resource-for-low-and-middle-income-countries-health-economics-practitioners-a-commentary
#13
Chiaki Urai Adeagbo, Waranya Rattanavipapong, Lorna Guinness, Yot Teerawattananon
Public health authorities around the world are increasingly using economic evaluation to set priorities and inform decision making in health policy, especially in the development of health benefit packages. Nevertheless, researchers in low- and middle-income countries (LMICs) encounter many barriers when conducting economic evaluations. In 2015, the Health Intervention and Technology Assessment Program identified key technical and context-specific challenges faced in conducting and using health economic evaluations in LMICs...
May 2018: Value in Health: the Journal of the International Society for Pharmacoeconomics and Outcomes Research
https://www.readbyqxmd.com/read/29752414/ligand-binding-and-activation-properties-of-the-purified-bacterial-cyclic-nucleotide-gated-channel-sthk
#14
Philipp A M Schmidpeter, Xiaolong Gao, Vikrant Uphadyay, Jan Rheinberger, Crina M Nimigean
Cyclic nucleotide-modulated ion channels play several essential physiological roles. They are involved in signal transduction in photoreceptors and olfactory sensory neurons as well as pacemaking activity in the heart and brain. Investigations of the molecular mechanism of their actions, including structural and electrophysiological characterization, are restricted by the availability of stable, purified protein obtained from accessible systems. Here, we establish that SthK, a cyclic nucleotide-gated (CNG) channel from Spirochaeta thermophila , is an excellent model for investigating the gating of eukaryotic CNG channels at the molecular level...
May 11, 2018: Journal of General Physiology
https://www.readbyqxmd.com/read/29750566/advanced-age-results-in-a-diminished-endothelial-glycocalyx
#15
Daniel R Machin, Samuel I Bloom, Robert A Campbell, Tam T T Phuong, Phillip Ellis Gates, Lisa A Lesniewski, Matthew T Rondina, Anthony John Donato
Age-related microvascular dysfunction is well characterized in rodents and humans, but little is known about the properties of the microvascular endothelial glycocalyx in advanced age. We examined the glycocalyx in microvessels of young and old C57BL6 mice (young: 6.1{plus minus}0.1 vs old: 24.6{plus minus}0.2 mo) using intravital microscopy and transmission electron microscopy, and in human participants (young: 27{plus minus}1 vs old: 61{plus minus}2 yr) using intravital microscopy. Glycocalyx thickness in mesenteric and skeletal muscle microvessels was 51-54% lower in old, compared to young mice...
May 11, 2018: American Journal of Physiology. Heart and Circulatory Physiology
https://www.readbyqxmd.com/read/29749223/organic-electrochemical-transistors-for-the-detection-of-cell-surface-glycans
#16
Lizhen Chen, Ying Fu, Naixiang Wang, Anneng Yang, Yuanzhe Li, Jie Wu, Huangxian Ju, Feng Yan
Cell surface glycans play critical roles in diverse biological processes, such as cell-cell communication, immunity, infection, development and differentiation. Their expressions are closely related to cancer growth and metastasis. This work demonstrates an organic electrochemical transistor (OECT) -based biosensor for the detection of glycan expression on living cancer cells. Using mannose on human breast cancer cells (MCF-7) as a target model, the gate electrode of the OECT is modified with concanavalin A that can specifically bind cell surface mannose and capture the cancer cells...
May 11, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29748663/direct-neurotransmitter-activation-of-voltage-gated-potassium-channels
#17
Rían W Manville, Maria Papanikolaou, Geoffrey W Abbott
Voltage-gated potassium channels KCNQ2-5 generate the M-current, which controls neuronal excitability. KCNQ2-5 subunits each harbor a high-affinity anticonvulsant drug-binding pocket containing an essential tryptophan (W265 in human KCNQ3) conserved for >500 million years, yet lacking a known physiological function. Here, phylogenetic analysis, electrostatic potential mapping, in silico docking, electrophysiology, and radioligand binding assays reveal that the anticonvulsant binding pocket evolved to accommodate endogenous neurotransmitters including γ-aminobutyric acid (GABA), which directly activates KCNQ5 and KCNQ3 via W265...
May 10, 2018: Nature Communications
https://www.readbyqxmd.com/read/29748486/transcriptomic-analysis-of-ciguatoxin-induced-changes-in-gene-expression-in-primary-cultures-of-mice-cortical-neurons
#18
Juan Andrés Rubiolo, Carmen Vale, Andrea Boente-Juncal, Masahiro Hirama, Shuji Yamashita, Mercedes Camiña, Mercedes R Vieytes, Luis M Botana
Ciguatoxins are polyether marine toxins that act as sodium channel activators. These toxins cause ciguatera, one of the most widespread nonbacterial forms of food poisoning, which presents several symptoms in humans including long-term neurological alterations. Earlier work has shown that both acute and chronic exposure of primary cortical neurons to synthetic ciguatoxin CTX3C have profound impacts on neuronal function. Thus, the present work aimed to identify relevant neuronal genes and metabolic pathways that could be altered by ciguatoxin exposure...
May 10, 2018: Toxins
https://www.readbyqxmd.com/read/29747403/development-of-a-wireless-unified-maintenance-system-for-the-structural-health-monitoring-of-civil-structures
#19
Gwanghee Heo, Byungjik Son, Chunggil Kim, Seunggon Jeon, Joonryong Jeon
A disaster preventive structural health monitoring (SHM) system needs to be equipped with the following abilities: First, it should be able to simultaneously measure diverse types of data (e.g., displacement, velocity, acceleration, strain, load, temperature, humidity, etc.) for accurate diagnosis. Second, it also requires standalone power supply to guarantee its immediate response in crisis (e.g., sudden interruption of normal AC power in disaster situations). Furthermore, it should be capable of prompt processing and realtime wireless communication of a huge amount of data...
May 9, 2018: Sensors
https://www.readbyqxmd.com/read/29747396/beta-estradiol-regulates-voltage-gated-calcium-channels-and-estrogen-receptors-in-telocytes-from-human-myometrium
#20
Adela Banciu, Daniel Dumitru Banciu, Cosmin Catalin Mustaciosu, Mihai Radu, Dragos Cretoiu, Junjie Xiao, Sanda Maria Cretoiu, Nicolae Suciu, Beatrice Mihaela Radu
Voltage-gated calcium channels and estrogen receptors are essential players in uterine physiology, and their association with different calcium signaling pathways contributes to healthy and pathological conditions of the uterine myometrium. Among the properties of the various cell subtypes present in human uterine myometrium, there is increasing evidence that calcium oscillations in telocytes (TCs) contribute to contractile activity and pregnancy. Our study aimed to evaluate the effects of beta-estradiol on voltage-gated calcium channels and estrogen receptors in TCs from human uterine myometrium and to understand their role in pregnancy...
May 9, 2018: International Journal of Molecular Sciences
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