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Nan Lu, Ting Wang, Pan Zhao, Lianjun Zhang, Xiaowen Lun, Xueli Zhang, Xiaohong Hou
In the presented work, metal-organic framework (MOF) material MIL-101(Cr) (MIL, Matérial Institute Lavoisier) was used as a sorbent for vortex assisted dispersive micro-solid-phase extraction (VA-D-μ-SPE) of trace amount of metronidazole (MNZ), ronidazole (RNZ), secnidazole (SNZ), dimetridazole (DMZ), tinidazole (TNZ), and ornidazole (ONZ) in different environmental water samples. Ultra-high-performance liquid chromatography coupled with tandem mass spectrometry (UPLC-MS/MS) was used to quantify the target analytes...
October 8, 2016: Analytical and Bioanalytical Chemistry
Nitya Sarjapuram, Niharika Mekala, Meetali Singh, Utpal Tatu
Travellers' diarrhoea caused by enteric protozoa like Entamoeba histolytica is among the most common protozoan diseases in developing countries. In developing countries, amoebiasis is the second most prevalent protozoan disease. This protozoan parasite is often known to coexist as a part of the normal gut microbiota. It is estimated that around 50-60 % of population in developing countries might be harbouring Entamoeba in an asymptomatic manner. Due to physiological perturbation or upon immuno-compromise, it can become virulent and then cause diarrhoea, bloody stools and may invade other organs if left untreated...
October 5, 2016: Probiotics and Antimicrobial Proteins
Daniele Maria Knupp de Souza, Claudio Galuppo Diniz, Didier Silveira Castellano Filho, Laura Maria Andrade de Oliveira, Débora Martins Coelho, Luciana De Souza Talha, Thiago César do Nascimento, Alessandra Barbosa Ferreira-Machado, Vânia Lúcia da Silva
INTRODUCTION: Bacterial vaginosis (BV) is a syndrome related to Gardnerella vaginalis and is characterized by an imbalance in the vaginal microbiota. This work focused on the evaluation of antimicrobial susceptibility patterns and the occurrence of the vaginolysin (vly) gene in G. vaginalis isolated from BV and non-BV patients. METHODOLOGY: The vaginal secretions were collected randomly and processed for G. vaginalis isolation. The isolates were presumptively identified by β-hemolysis and oxidase and catalase tests...
September 30, 2016: Journal of Infection in Developing Countries
Dariana Pimentel Gomes Hübner, Patrícia de Brum Vieira, Amanda Piccoli Frasson, Camila Braz Menezes, Franciane Rios Senger, Gloria Narjara Santos da Silva, Simone Cristina Baggio Gnoatto, Tiana Tasca
Caused by Trichomonas vaginalis, trichomoniasis is the most common non-viral STD worldwide. Currently, metronidazole and tinidazole are the only drugs approved for treatment of the condition. However, problems such as metronidazole-resistant T. vaginalis isolates and allergic reactions have been reported. Based on data previously published by our group, structural changes in betulinic acid (1) were performed, generating three new compounds that were tested for in vitro anti-T.vaginalis activity in this study...
September 26, 2016: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
Jin Guan, Chi Zhang, Yang Wang, Yiguang Guo, Peiting Huang, Longshan Zhao
A new analytical method was developed for simultaneous determination of 12 pharmaceuticals using ultrasound-assisted dispersive liquid-liquid microextraction (DLLME) followed by ultra-high performance liquid chromatography with tandem mass spectrometry (UHPLC-MS/MS). Six nonsteroidal anti-inflammatory drugs (NSAIDs, ketoprofen, mefenamic acid, tolfenamic acid, naproxen, sulindac, and piroxicam) and six antibiotics (tinidazole, cefuroxime axetil, ciprofloxacin, sulfamethoxazole, sulfadiazine, and chloramphenicol) were extracted by ultrasound-assisted DLLME using dichloromethane (800 μL) and methanol/acetonitrile (1:1, v/v, 1200 μL) as the extraction and dispersive solvents, respectively...
September 10, 2016: Analytical and Bioanalytical Chemistry
Amol P Muthal, Supada R Rojatkar, Subhash Laxmanrao Bodhankar
BACKGROUND: γ-oryzanol is a major bioactive constituent in rice. Most of the literature reports isolation of 24-methylenecycloartanyl ferulate (24-mCAF) from rice bran oil (RBO) of other than Indian variety. Current research has successfully applied high-performance thin layer chromatography (HPTLC) method for isolation of 24-mCAF from Indian variety (Indrayani) of RBO. MATERIALS AND METHODS: HPTLC method was developed for standard γ-oryzanol using tinidazole as an internal standard...
May 2016: Pharmacognosy Magazine
Jianfei Tao, Ling Mao, Bailun Zhou, Qun Liu, Aigang Yang, Guobing Wei, Runhui Liu, Wei-Dong Zhang, Wen Xu, Ji Ye
A sensitive and reliable bioanalytical method was established for quantitative and pharmacokinetic investigation of nine ginsenosides and seven bufadienolides in rat plasma after the oral administration of Shexiang Baoxin Pill (SBP) by liquid chromatography-electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS), using tinidazole and digoxin as internal standards (ISTDs). All of the analytes and ISTDs could obtain satisfactory recoveries by solid-phase extraction using Oasis HLB μElution Plate, which was eluted with methanol and ethyl acetate successively, and chromatographic separation was achieved on a Shim-pack XR-ODSIIcolumn (75 × 2...
August 16, 2016: Biomedical Chromatography: BMC
Jie Feng, Wanliang Shi, Shuo Zhang, David Sullivan, Paul G Auwaerter, Ying Zhang
Although currently recommended antibiotics for Lyme disease such as doxycycline or amoxicillin cure the majority of the patients, about 10-20% of patients treated for Lyme disease may experience lingering symptoms including fatigue, pain, or joint and muscle aches. Under experimental stress conditions such as starvation or antibiotic exposure, Borrelia burgdorferi can develop round body forms, which are a type of persister bacteria that appear resistant in vitro to customary first-line antibiotics for Lyme disease...
2016: Frontiers in Microbiology
Noha Ferrah, Karin Leder, Katherine Gibney
BACKGROUND: Thirty to seventy per cent of overseas travellers experience traveller's diarrhoea (TD), a potential cause of serious gastrointestinal (GI) sequelae. However, there is limited evidence on the optimal management of TD. OBJECTIVE: The objectives of this article are to characterise the aetiologies and management of returned travellers with ongoing GI symptoms referred to a specialist infectious diseases service. METHODS: We conducted a retrospective medical record review of patients referred to the Victorian Infectious Disease Service (VIDS) in 2013-15 with a history of overseas travel and GI symptoms present for longer than two weeks...
May 2016: Australian Family Physician
Yosef Nikodimos, Meareg Amare
Cyclic voltammetric investigation of metronidazole at carbon paste electrode revealed an irreversible reduction peak centered at about -0.4 V. Observed peak potential shift with pH in the range 2.0 to 8.5 indicated the involvement of protons during the reduction of metronidazole, whereas the peak potential shift with scan rate in the range 10-250 mV/s confirmed the irreversibility of the reduction reaction. A better correlation coefficient for the dependence of peak current on the scan rate than on the square root of scan rate indicated an adsorption controlled kinetics...
2016: Journal of Analytical Methods in Chemistry
Braj Gaurav Sharma, Neeraj Kumar, Dhruv Kumar Nishad, Naveen K Khare, Aseem Bhatnagar
Tinidazole is a versatile anti-amoebic and anti-anaerobic drug used in treatment of intestinal infection. The aim of present study was to develop and evaluate a guar gum based novel target release Tinidazole matrix tablet in animal models and healthy human volunteer using Gamma Scintigraphy technique. Anti-anaerobic and anti-protozoal activity of the developed formulation was studied in vitro against Bacteroides fragilis and Dentamoeba fragilis. Tinidazole was successful radiolabelled with (99m)Tc-pertechnetate using stannous chloride as a reducing agent and stable up to 24h in normal saline and serum...
June 30, 2016: European Journal of Pharmaceutical Sciences
Atieh Makhlough, Hafez Fakheri, Samaneh Hojati, Vahid Hosseini, Zohreh Bari
BACKGROUND The prevalence of peptic ulcer disease in hemodialysis patients is more than the general population. They are also more prone to complications including upper gastrointestinal bleeding. The aim of this study was to compare the efficacy of 14 days hybrid regimen with 14 days triple therapy for Helicobacter pylori (H. pylori ) eradication in hemodialysis patients. METHODS Forty hemodialysis patients with naïve H.pylori infection were randomized to receive either hybrid regimen (pantoprazole 40 mg + amoxicillin 500 mg, both twice a day during the first 7 days, followed by pantoprazole 40 mg + amoxicillin 500 mg + clarithromycin 500 mg + tinidazole 500 mg, all twice a day, for the second 7 days, or standard triple therapy including pantoprazole 40 mg, clarithromycin 500 mg, and amoxicillin 500 mg, all twice a day for 14 days...
January 2016: Middle East Journal of Digestive Diseases
F Franceschi, V Ojetti, M Gabrielli, C Petruzziello, A Tortora, G Gasbarrini, L R Lopetuso, F Scaldaferri, A Gasbarrini
OBJECTIVE: Helicobater (H.) pylori eradication rates with standard first-line triple therapy have declined to unacceptable levels. To date, amoxicillin-resistant H. pylori strains have rarely been detected. Whether increasing the dosage of amoxicillin in a standard 7 days eradicating regimen may enhance its efficacy is not known. The aim of this paper is to compare the efficacy of a 7 days high-dose amoxicillin based first-line regimen with sequential therapy. PATIENTS AND METHODS: We have retrospectively analyzed data from 300 sex and age matched patients, who underwent 3 different therapeutic schemes: (1) standard LCA, lansoprazole 30 mg bid, clarithromycin 500 mg bid and amoxicillin 1000 mg bid for 7 days; (2) high dose LCA (HD-LCA), lansoprazole 30 mg bid, clarithromycin 500 mg bid and amoxicillin 1000 mg tid for 7 days; (3) sequential LACT, lansoprazole 30 mg bid plus amoxicillin 1000 mg bid for 5 days, followed by lansoprazole 30 mg bid, clarithromycin 500 mg bid and tinidazole 500 mg bid for 5 days...
2016: European Review for Medical and Pharmacological Sciences
Zhiqiang Song, Liya Zhou, Jianzhong Zhang, Lihua He, Peng Bai, Yan Xue
BACKGROUND: Hybrid therapy has recently attracted widespread attention. However, many issues require further exploration. For example, research in regions with high antibiotic resistance rates is limited, and the correlation between eradication efficacy and antibiotic resistance remains unclear. The aim of this study was to determine the efficacy, compliance, safety, and risk factors of hybrid therapy as first-line regimen in a region with high antibiotic resistance rates. MATERIALS AND METHODS: This prospective study was conducted in a tertiary hospital between January 2014 and June 2015...
October 2016: Helicobacter
Ning Liang, Peiting Huang, Xiaohong Hou, Zhen Li, Lei Tao, Longshan Zhao
A novel method, solid-phase extraction combined with dispersive liquid-liquid microextraction (SPE-DLLME), was developed for ultra-preconcentration of 10 antibiotics in different environmental water samples prior to ultra-high performance liquid chromatography-tandem mass spectrometry detection. The optimized results were obtained as follows: after being adjusted to pH 4.0, the water sample was firstly passed through PEP-2 column at 10 mL min(-1), and then methanol was used to elute the target analytes for the following steps...
February 2016: Analytical and Bioanalytical Chemistry
Peiting Huang, Pan Zhao, Xinpeng Dai, Xiaohong Hou, Longshan Zhao, Ning Liang
A novel pretreatment method involving microwave-assisted extraction and solid-phase purification combined with dispersive liquid-liquid microextraction (MAE-SPP-DLLME) followed by ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) was established for the simultaneous determination of six antibacterial pharmaceuticals including metronidazole, tinidazole, chloramphenicol, thiamphenicol, malachite green and crystal violet. The conditions of MAE were optimized using an orthogonal design and the optimal conditions were found to be 8mL for acetonitrile, 50°C for 5min...
February 1, 2016: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Chung-Chuan Chan, Nai-Hsuan Chien, Chia-Long Lee, Yi-Chen Yang, Chih-Sheng Hung, Tien-Chien Tu, Chi-Hwa Wu
BACKGROUND: Eradication rates of standard triple therapy for Helicobacter pylori infections have decreased in recent years due to a worldwide increase in bacterial resistance. Sequential therapy has the advantage of a two-phase treatment regimen and achieves a superior result for H. pylori eradication in peptic ulcer disease. However, no study has yet compared the efficacy of sequential therapy for H. pylori eradication exclusively in inactive duodenal ulcer (iDU) or non-ulcer dyspepsia (NUD)...
2015: BMC Gastroenterology
Siddharth Singh, Andrea M Stroud, Stefan D Holubar, William J Sandborn, Darrell S Pardi
BACKGROUND: Pouchitis occurs in approximately 50% of patients following ileal pouch-anal anastomosis (IPAA) for chronic ulcerative colitis. OBJECTIVES: The primary objective was to determine the efficacy and safety of medical therapies (including antibiotics, probiotics, and other agents) for prevention or treatment of acute or chronic pouchitis. SEARCH METHODS: We searched MEDLINE, EMBASE and the Cochrane Library from inception to October 2014...
2015: Cochrane Database of Systematic Reviews
Jung Mi Byun, Dae Hoon Jeong, Young Nam Kim, Kyung Bok Lee, Moon Su Sung, Ki Tae Kim
OBJECTIVE: There are no universally successful guidelines for the treatment of metronidazole-resistant vaginal trichomoniasis. This is distressing for patients and frustrating for physicians. We therefore decided to evaluate whether zinc sulfate douche is effective in treating vaginal trichomoniasis, because the compound is a natural antimicrobial chemical defense in humans. CASE REPORTS: In our retrospective case review, eight cases of metronidazole-resistant trichomoniasis were treated with 1% zinc sulfate douche with or without tinidazole between 2005 and 2012...
October 2015: Taiwanese Journal of Obstetrics & Gynecology
Angel A Escobedo, Javier Ballesteros, Eduardo González-Fraile, Pedro Almirall
Metronidazole is frequently used against Giardia infection; however, it has been associated with significant failure rates in clearing parasites from the gut; additionally, as it should be taken for 5 to 10 days, it is associated with poor compliance, probably due to side effects. Other drugs, including tinidazole (TNZ) and albendazole (ABZ) have been included in the antigiardial armamentarium. Our aim was to assess the efficacy of ABZ compared with TNZ in Giardia infections in children. A systematic review and a meta-analysis were carried out...
January 2016: Acta Tropica
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