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https://www.readbyqxmd.com/read/28813704/p38-kinase-sgk1-and-nf-%C3%AE%C2%BAb-dependent-up-regulation-of-na-ca2-exchanger-expression-and-activity-following-tgf%C3%A3-1-treatment-of-megakaryocytes
#1
Tamer Al-Maghout, Lisann Pelzl, Itishri Sahu, Basma Sukkar, Zohreh Hosseinzadeh, Ravi Gutti, Stefan Laufer, Jakob Voelkl, Burkert Pieske, Meinrad Gawaz, Florian Lang
BACKGROUND: TGFβ1, a decisive regulator of megakaryocyte maturation and platelet formation, has previously been shown to up-regulate both, store operated Ca2+ entry (SOCE) and Ca2+ extrusion by Na+/Ca2+ exchange. The growth factor thus augments the increase of cytosolic Ca2+ activity ([Ca2+]i) following release of Ca2+ from intracellular stores and accelerates the subsequent decline of [Ca2+]i. The effect on SOCE is dependent on a signaling cascade including p38 kinase, serum & glucocorticoid inducible kinase SGK1, and nuclear factor NFκB...
August 15, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28812045/soluble-st2-predicts-outcome-and-hemorrhagic-transformation-after-acute-stroke
#2
Zoe Wolcott, Ayush Batra, Matthew B Bevers, Cristina Sastre, Jane Khoury, Matthew Sperling, Brett C Meyer, Kyle B Walsh, Opeolu Adeoye, Joseph P Broderick, W Taylor Kimberly
OBJECTIVE: ST2 is a member of the toll-like receptor superfamily that can alter inflammatory signaling of helper T-cells. We investigated whether soluble ST2 (sST2) could independently predict outcome and hemorrhagic transformation (HT) in the setting of stroke. METHODS: We measured sST2 in patients enrolled in the Specialized Program of Translational Research in Acute Stroke (SPOTRIAS) network biomarker study. 646 patients had plasma samples collected at the time of hospital admission and 210 patients had a second sample collected 48 h after stroke onset...
August 2017: Annals of Clinical and Translational Neurology
https://www.readbyqxmd.com/read/28811527/atherosusceptible-shear-stress-activates-endoplasmic-reticulum-stress-to-promote-endothelial-inflammation
#3
Keith A Bailey, Fawaz G Haj, Scott I Simon, Anthony G Passerini
Atherosclerosis impacts arteries where disturbed blood flow renders the endothelium susceptible to inflammation. Cytokine activation of endothelial cells (EC) upregulates VCAM-1 receptors that target monocyte recruitment to atherosusceptible regions. Endoplasmic reticulum (ER) stress elicits EC dysregulation in metabolic syndrome. We hypothesized that ER plays a central role in mechanosensing of atherosusceptible shear stress (SS) by signaling enhanced inflammation. Aortic EC were stimulated with low-dose TNFα (0...
August 15, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28810809/optimization-of-human-nk-cell-manufacturing-fully-automated-separation-improved-i-ex-vivo-i-expansion-using-il-21-with-autologous-feeder-cells-and-generation-of-anti-cd123-car-expressing-effector-cells
#4
Stephan Klöß, Olaf Oberschmidt, Michael Morgan, Julia Dahlke, Lubomir Arseniev, Volker Huppert, Markus Granzin, Tanja Gardlowski, Nadine Matthies, Stefanie Soltenborn, Axel Schambach, Ulrike Koehl
BACKGROUND AND AIMS: The administration of ex-vivo expanded Natural killer (NK) cells as potential antitumor effector cells appears to be suitable for effector cell-based immunotherapies in high-risk cancer patients. However, the GMP-conform manufacturing of clinical-grade NK cells at sufficiently high numbers represent a great challenge. Therefore, we improved and optimized previous expansion protocols for those effector cells through the use of newly developed culture medium, IL-21 and autologous feeder cells...
August 16, 2017: Human Gene Therapy
https://www.readbyqxmd.com/read/28810254/insulin-influences-lps-induced-tnf-%C3%AE-and-il-6-release-through-distinct-pathways-in-mouse-macrophages-from-different-compartments
#5
Fernando H G Tessaro, Thais S Ayala, Eduardo L Nolasco, Leonardo M Bella, Joilson O Martins
BACKGROUND/AIMS: Diabetic subjects are more susceptible to infections, which is partially due to insulin deficiency and hyperglycemia. We hypothesized that insulin influences cytokine release by macrophages from diabetic C57BL/6 mice stimulated with lipopolysaccharides (LPS). METHODS: Bone marrow-derived macrophages (BMDM) and tissue-specific macrophages from diabetic (alloxan 60 mg/kg, i.v.) male C57BL/6 mice were stimulated by LPS (100 ng/mL) and/or treated by insulin (1 mU/mL)...
August 15, 2017: Cellular Physiology and Biochemistry
https://www.readbyqxmd.com/read/28809446/deferasirox-for-managing-iron-overload-in-people-with-thalassaemia
#6
REVIEW
Claudia Bollig, Lisa K Schell, Gerta Rücker, Roman Allert, Edith Motschall, Charlotte M Niemeyer, Dirk Bassler, Joerg J Meerpohl
BACKGROUND: Thalassaemia is a hereditary anaemia due to ineffective erythropoiesis. In particular, people with thalassaemia major develop secondary iron overload resulting from regular red blood cell transfusions. Iron chelation therapy is needed to prevent long-term complications.Both deferoxamine and deferiprone are effective; however, a review of the effectiveness and safety of the newer oral chelator deferasirox in people with thalassaemia is needed. OBJECTIVES: To assess the effectiveness and safety of oral deferasirox in people with thalassaemia and iron overload...
August 15, 2017: Cochrane Database of Systematic Reviews
https://www.readbyqxmd.com/read/28807092/extraction-and-determination-of-trace-amounts-of-p-coumaric-acid-in-vinegar-carrot-juice-and-seed-extract-from-silybum-marianum-l-gaertn
#7
Rouhollah Khani, Zeinab Rostami, Ghodsieh Bagherzade, Vahid Khojeh
In this study, for the monitoring and quantification of p-coumaric acid (p-CA) in vinegar, carrot juice, and seed extract from the plant species Silybum marianum (L.) Gaertn, an efficient and low-cost analytical method has been applied. For this purpose, a dispersive liquid-liquid microextraction (DLLME) method, followed by UV-Vis spectrophotometric detection, was used. To form a cloudy solution, a binary mixture containing ethanol as a disperser solvent and chloroform as an extraction solvent was rapidly injected by syringe into a sample solution containing p-CA...
August 10, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28803769/urine-biomarkers-of-tubular-renal-cell-damage-for-the-prediction-of-acute-kidney-injury-after-cardiac-surgery-a-pilot-study
#8
Tanja Mayer, Daniel Bolliger, Markus Scholz, Oliver Reuthebuch, Michael Gregor, Patrick Meier, Martin Grapow, Manfred D Seeberger, Jens Fassl
OBJECTIVE: To evaluate the perioperative course of urine levels of the renal damage biomarkers tissue inhibitor of metalloproteinase 2 (TIMP-2) and insulin-like growth factor-binding protein 7 (IGFBP7) and to evaluate the predictive value of elevated TIMP-2 × IGFBP7 concentrations to predict acute kidney injury (AKI) early after cardiac on-pump surgery. DESIGN: Prospective, observational cohort study. SETTING: University hospital. PARTICIPANTS: The study comprised 110 consecutive patients undergoing elective cardiac surgery with cardiopulmonary bypass (CPB) between January and March 2014...
April 16, 2017: Journal of Cardiothoracic and Vascular Anesthesia
https://www.readbyqxmd.com/read/28801891/monitoring-of-busulphan-concentrations-in-children-undergone-hematopoietic-stem-cell-transplantation-unicentric-experience-over-10%C3%A2-years
#9
Maura Faraci, Carmine Tinelli, Edoardo Lanino, Stefano Giardino, Massimiliano Leoni, Marta Ferretti, Elio Castagnola, Monica Broglia, Annalisa De Silvestri, Daniela Di Martino, Antonella Bartoli
BACKGROUND AND OBJECTIVES: The aim of this report is to describe the experience in the management of busulphan-based conditioning regimen administered before hematopoietic stem cell transplantation (HSCT) in children. METHODS: We report the values of the first dose AUC (area under the concentration-time curve, normal target between 3600 and 4800 ng·h/mL) in children treated with oral and intravenous busulphan, and we analyze the impact of some clinical variables in this cohort of patients...
August 11, 2017: European Journal of Drug Metabolism and Pharmacokinetics
https://www.readbyqxmd.com/read/28800637/biologically-active-recombinant-human-erythropoietin-expressed-in-hairy-root-cultures-and-regenerated-plantlets-of-nicotiana-tabacum-l
#10
Poornima Devi Gurusamy, Holger Schäfer, Siva Ramamoorthy, Michael Wink
Hairy root culture is a potential alternative to conventional mammalian cell culture to produce recombinant proteins due to its ease in protein recovery, low costs and absence of potentially human pathogenic contaminants. The current study focussed to develop a new platform of a hairy root culture system from Nicotiana tabacum for the production of recombinant human EPO (rhEPO), which is regularly produced in mammalian cells. The human EPO construct was amplified with C-terminal hexahistidine tag from a cDNA of Caco-2 cells...
2017: PloS One
https://www.readbyqxmd.com/read/28800554/anthraquinone-2-sulfonate-immobilized-to-conductive-polypyrrole-hydrogel-as-a-bioanode-to-enhance-power-production-in-microbial-fuel-cell
#11
Xinhua Tang, How Yong Ng
In this study, anthraquinone-2-sulfonate (AQS), a redox mediator, was covalently bound to conductive polypyrrole hydrogel (CPH) via electrochemical reduction of the in-situ-generated AQS diazonium salts. The porous structure and hydrophilic surface of this CPH/AQS anode enhanced biofilm formation while the AQS bound on the CPH/AQS anode worked as a redox mediator. The CPH/AQS bioanode reduced the charge transfer resistance from 28.3Ω to 4.1Ω while increased the maximum power density from 762±37mW/m(2) to 1919±69mW/m(2), compared with the bare anode...
August 4, 2017: Bioresource Technology
https://www.readbyqxmd.com/read/28795668/-immune-intervention-of-human-umbilical-cord-mesenchymal-stem-cells-on-sepsis-rats
#12
Hewei Zhang, Xiaoxu Cui, Tao Fang, Qiang Fu
OBJECTIVE: To investigate the effect of human umbilical cord mesenchymal stem cells (UC-MSCs) on immune cells and inflammatory factors in septic rats. METHODS: 184 male Sprague-Dawley (SD) rats were divided into normal control group (n = 8), sham operation group (n = 48), sepsis model group (n = 64), and UC-MSCs treatment group (n = 64). An animal model of sepsis was produced by cecal ligation and puncture (CLP). In the UC-MSCs treatment group 1 mL UC-MSCs (2×10(6)/mL) were injected intraperitoneally at 1 hour after the model establishment; the sham operation group and the sepsis model group were given the same amount of saline...
August 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28795663/-evaluation-function-of-intermedin-on-prognosis-of-elderly-patients-with-sepsis
#13
Yi Zhang, Fengxue Zhu, Weihuan Du, Yongmei He, Li Guo, Zhihong Yan, Hong Ji
OBJECTIVE: To investigate the predicting value of intermedin (IMD) for the prognosis of elderly sepsis patients. METHODS: A retrospective analysis was conducted. Forty-one patients with sepsis, aged ≥65 years, and admitted to geriatrics intensive care unit of Aerospace Center Hospital from April 2015 to December 2016 were enrolled. Thirty healthy patients were studied as control during the same time. The expression of C-reactive protein (CRP), procalcitonin (PCT) and IMD were tested within 24 hours during hospitalization, and the acute physiology and chronic health evaluation II (APACHE II) score and prognosis was evaluated...
August 2017: Zhonghua Wei Zhong Bing Ji Jiu Yi Xue
https://www.readbyqxmd.com/read/28791590/heterologous-expression-of-aldehyde-dehydrogenase-in-lactococcus-lactis-for-acetaldehyde-detoxification-at-low-ph
#14
Yunbin Lyu, Gisèle LaPointe, Lei Zhong, Jing Lu, Chong Zhang, Zhaoxin Lu
Aldehyde dehydrogenase (E.C. 1.2.1.x) can catalyze detoxification of acetaldehydes. A novel acetaldehyde dehydrogenase (istALDH) from the non-Saccharomyces yeast Issatchenkia terricola strain XJ-2 has been previously characterized. In this work, Lactococcus lactis with the NIsin Controlled Expression (NICE) System was applied to express the aldehyde dehydrogenase gene (istALDH) in order to catalyze oxidation of acetaldehyde at low pH. A recombinant L. lactis NZ3900 was obtained and applied for the detoxification of acetaldehyde as whole-cell biocatalysts...
August 8, 2017: Applied Biochemistry and Biotechnology
https://www.readbyqxmd.com/read/28791357/synergistic-effects-of-fibroblast-growth-factor-2-and-bone-morphogenetic-protein-2-on-bone-induction
#15
Rongying Song, Dingding Wang, Rong Zeng, Ju Wang
The present study investigated the synergistic effect of co‑administering fibroblast growth factor‑2 (FGF‑2) and bone morphogenetic protein‑2 (BMP‑2) on osteoblastic differentiation in C2C12 cells and in rats. C2C12 murine myoblast cells represent a well‑accepted in vitro model system to study the ability of BMP‑2 to alter cell lineage from the myogenic to the osteogenic phenotype. The osteoblastic differentiation potency was determined by alkaline phosphatase (ALP) and Alizarin red S staining...
August 7, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28791020/p2x7-receptor-induces-tumor-necrosis-factor-%C3%AE-converting-enzyme-activation-and-release-to-boost-tnf-%C3%AE-production
#16
Maria Barberà-Cremades, Ana I Gómez, Alberto Baroja-Mazo, Laura Martínez-Alarcón, Carlos M Martínez, Carlos de Torre-Minguela, Pablo Pelegrín
Tumor necrosis factor (TNF)-α is a major pro-inflammatory cytokine produced in response to toll-like receptor stimulation. TNF-α release is controlled by the activity of TNF-α converting enzyme (TACE) that cut membrane-bound TNF-α to shed its ectodomain as a soluble cytokine. The purinergic receptor P2X ligand-gated ion channel 7 (P2X7) is activated in response to elevated concentrations of extracellular ATP and induces different pro-inflammatory pathways in macrophages to establish an inflammatory response...
2017: Frontiers in Immunology
https://www.readbyqxmd.com/read/28790247/-pulmonary-mucoepidermoid-carcinoma-with-hilar-lymph-node-metastasis-complicated-by-myelodysplastic-syndrome
#17
Yoshinari Matsuda, Hiroshi Moriyama, Masashi Inaba, Yoshihiko Kubo, Masahiro Kitada, Satoshi Hayashi, Kei Ishibashi, Satoshi Okazaki, Nana Takahashi, Shunsuke Yasuda, Masahiro Abe, Satoshi Hirata
A 65-year-old woman was found to have a mass shadow on chest computed tomography. The patient had been diagnosed as having myelodysplastic syndrome 3 years before admission. She was transferred to our hospital for further examination of the mass in the lower lobe of left lung. The mass was positron emission tomography-positive (SUVmax of 8.6)suggesting a malignant neoplasm. Serum concentrations of carcinoembryonic antigen was elevated to 8.7 ng/ml. Preoperative laboratory studies showed anemia (hemoglobin 6...
August 2017: Kyobu Geka. the Japanese Journal of Thoracic Surgery
https://www.readbyqxmd.com/read/28782189/epidermal-growth-factor-receptor-detection-in-serum-and-saliva-as-a-diagnostic-and-prognostic-tool-in-oral-cancer
#18
Laura Zanotti, Alberto Paderno, Cesare Piazza, Eleonora Pagan, Eliana Bignotti, Chiara Romani, Elisabetta Bandiera, Stefano Calza, Francesca Del Bon, Piero Nicolai, Antonella Ravaggi
OBJECTIVES/HYPOTHESIS: Epidermal growth factor receptor (EGFR) is a type I transmembrane glycoprotein that is overexpressed in a wide variety of malignancies, including oral squamous cell carcinoma (OSCC). Our objective was to assess the EGFR diagnostic and prognostic value in OSCC by investigating its expression in serum and saliva of patients in comparison with healthy subjects. STUDY DESIGN: Prospective case-control study. METHODS: Serum and saliva samples were collected from a cohort of 63 treatment-naïve OSCC patients before surgery and a matched group of 60 healthy subjects...
August 7, 2017: Laryngoscope
https://www.readbyqxmd.com/read/28780891/vitamin-d-and-corticotropin-releasing-hormone-in-term-and-preterm-birth-potential-contributions-to-preterm-labor-and-birth-outcome
#19
Sara A Mohamed, Abdeljabar El Andaloussi, Ayman Al-Hendy, Ramkumar Menon, Faranak Behnia, Jay Schulkin, Michael L Power
BACKGROUND: Poor maternal vitamin D status and elevated circulating corticotropin-releasing hormone (CRH) are associated with preterm birth. It is not known if these risk factors are independent or interrelated. Both are associated with inflammation. METHODS: We measured maternal circulating 25-hydroxyvitamin D (25-OH-D) and CRH from 97 samples collected from 15 early-preterm, 31 late-preterm, 21 early-term, and 30 term births. The potential involvement of vitamin D in the regulation of inflammation was evaluated by Q-PCR in human uterine smooth muscle (UTSM) cell line...
August 7, 2017: Journal of Maternal-fetal & Neonatal Medicine
https://www.readbyqxmd.com/read/28780131/commentary-on-clonal-evolution-of-chemotherapy-resistant-urothelial-carcinoma-faltas-bm-prandi-d-tagawa-st-molina-am-nanus-dm-sternberg-c-rosenberg-j-mosquera-jm-robinson-b-elemento-o-sboner-a-beltran-h-demichelis-f-rubin-ma-nat-genet-2016-oct-17-http-dx-doi
#20
Byron H Lee
Chemotherapy-resistant urothelial carcinoma has no uniformly curative therapy. Understanding how selective pressure from chemotherapy directs the evolution of urothelial carcinoma and shapes its clonal architecture is a central biological question with clinical implications. To address this question, we performed whole-exome sequencing and clonality analysis of 72 urothelial carcinoma samples, including 16 matched sets of primary and advanced tumors prospectively collected before and after chemotherapy. Our analysis provided several insights that are as follows: (1) chemotherapy-treated urothelial carcinoma is characterized by intrapatient mutational heterogeneity, and most mutations are not shared; (2) both branching evolution and metastatic spread are very early events in the natural history of urothelial carcinoma; (3) chemotherapy-treated urothelial carcinoma is enriched with clonal mutations involving L1 cell-adhesion molecule and integrin signaling pathways; and (4) APOBEC-induced mutagenesis is clonally enriched in chemotherapy-treated urothelial carcinoma and continues to shape the evolution of urothelial carcinoma throughout its lifetime...
August 2, 2017: Urologic Oncology
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