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https://www.readbyqxmd.com/read/28445259/the-role-of-stat3-and-ahr-in-the-differentiation-of-cd4-t-cells-into-th17-and-treg-cells
#1
Xingxing Liu, Hui Hu, Heng Fan, Dongmei Zuo, Zhexing Shou, Yi Liao, Zhen Nan, Qing Tang
BACKGROUND: This study aimed to investigate the role of aryl hydrocarbon receptor (AhR) and STAT3 gene during the differentiation of cluster of differentiation (CD)4 T cells into T helper (Th)17 and T regulatory (Treg) cells. METHODS: First, CD4 T cells were isolated from the spleen of BALB/c mice. Then, stable CD4 T cells expressing STAT3 shRNA were constructed. CD4 T cells were assigned to one of the following treatments: Th17 group: antibodies against CD3 and CD28, 2...
April 2017: Medicine (Baltimore)
https://www.readbyqxmd.com/read/28443261/diabetes-mellitus-caused-by-secondary-hemochromatosis-after-multiple-blood-transfusions-in-2-patients-with-severe-aplastic-anemia
#2
Hyun Jin Kim, Yoon-Myung Kim, Eungu Kang, Beom Hee Lee, Jin-Ho Choi, Han-Wook Yoo
Hemochromatosis is an inherited or secondary disorder caused by excessive iron storage leading to multiple organ damage. We describe 2 patients with diabetes mellitus caused by hemochromatosis secondary to multiple blood transfusions due to severe aplastic anemia. Subject 1, who was diagnosed with severe aplastic anemia at 15 years of age, received multiple red blood cell transfusions before he underwent autologous peripheral blood stem cell transplantation (PBSCT) at 22 years of age. At 21 years of age, hyperglycemia was detected with increased hemoglobin A1c and serum ferritin levels, 9...
March 2017: Annals of Pediatric Endocrinology & Metabolism
https://www.readbyqxmd.com/read/28441052/effects-of-hyaluronan-alone-or-in-combination-with-chondroitin-sulfate-and-n-acetyl-d-glucosamine-on-lipopolysaccharide-challenge-exposed-equine-fibroblast-like-synovial-cells
#3
Allison H Kilborne, Hayam Hussein, Alicia L Bertone
OBJECTIVE To investigate effects of hyaluronic acid (HA) or HA combined with chondroitin sulfate (CS) and N-acetyl-d-glucosamine (NAG) by use of a lipopolysaccharide (LPS) in vitro method. SAMPLE Monolayer cultures of synovial cells from 4 adult horses. PROCEDURES Synovial cell cultures were untreated or treated with HA alone or HA-CS-NAG for 24 hours, subsequently unchallenged or challenge-exposed with 2 LPS concentrations (20 and 50 ng/mL) for 2 hours, and retreated with HA or HA-CS-NAG for another 24 hours...
May 2017: American Journal of Veterinary Research
https://www.readbyqxmd.com/read/28440509/modulation-of-mmp-2-and-9-secretion-by-cytokines-inducers-and-inhibitors-in-human-melanoma-a-2058-cells
#4
M Waheed Roomi, Tatiana Kalinovsky, Aleksandra Niedzwiecki, Matthias Rath
Melanoma, an extremely aggressive cancer, causes the most skin cancer-related deaths, due to metastasis to other areas of the body, such as lymph nodes, lungs, liver, brain or bone. It is characterized by high levels of matrix metalloproteinase (MMP)-2 and -9 secretions that degrade the extracellular matrix and basement membrane, allowing cancer cells to spread to distal organs. Various cytokines, mitogens, growth factors, inducers and inhibitors control MMP activities. We investigated the roles of these in regulation of MMP-2 and -9 in human melanoma A-2058 cells...
April 24, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28440429/cathepsin%C3%A2-b-expression-in-colorectal-cancer-in-a-middle-east-population-potential-value-as-a-tumor-biomarker-for-late-disease-stages
#5
Maha-Hamadien Abdulla, Mansoor-Ali Valli-Mohammed, Khayal Al-Khayal, Abdulmalik Al Shkieh, Ahmad Zubaidi, Rehan Ahmad, Khalid Al-Saleh, Omar Al-Obeed, James McKerrow
Cathepsin B (CTSB), is a cysteine protease belonging to the cathepsin (Clan CA) family. The diagnostic and prognostic significance of increased CTSB in the serum of cancer patients have been evaluated for some tumor types. CTSB serum and protein levels have also been reported previously in colorectal cancer (CRC) with contradictory results. The aim of the present study was to investigate CTSB expression in CRC patients and the association of CTSB expression with various tumor stages in a Middle East population...
April 18, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28439497/study-of-recombinant-human-interleukin-12-for-treatment-of-complications-after-radiotherapy-for-tumor-patients
#6
Na Guo, Wen-Qin Wang, Xiao-Jing Gong, Lei Gao, Li-Rong Yang, Wei-Na Yu, Hong-Yu Shen, Ling-Qin Wan, Xi-Feng Jia, Yi-Shan Wang, Yi Zhao
AIM: To evaluate the treatment effects of recombinant human interleukin-12 (rhIL-12) on radiotherapy complications, such as severe myelosuppression or pancytopenia, the decline or imbalance of immune function, etc. METHODS: The patients received high-dose and short-course precise radiotherapy, such as Cyber knife and image-guided radiotherapy (IGRT), which can cause myelosuppression or pancytopenia and immune function decline within a short time. One-hundred subjects were enrolled in the study, and 50 were randomized to a treatment group which used rhIL-12 and 50 were randomized to a control group which used symptomatic and supportive therapy after radiotherapy...
April 10, 2017: World Journal of Clinical Oncology
https://www.readbyqxmd.com/read/28438974/b-anthracis-edema-toxin-increases-fractional-free-water-and-sodium-reabsorption-in-an-isolated-perfused-rat-kidney-model
#7
Dharmvir S Jaswal, Xizhong Cui, Parizad Torabi-Parizi, Lernik Ohanjanian, Hannish Sampath-Kumar, Yvonne Fitz, Yan Li, Wanying Xu, Peter Q Eichacker
Introduction:B. anthracis edema toxin (ET) consists of protective antigen (PA), necessary for host cell toxin uptake, and edema factor (EF), the toxic moiety which increases host cell cAMP. Since vasopressin stimulates renal water and sodium reabsorption via increased tubular cell cAMP, we hypothesized ET would also. To test this hypothesis, we employed an isolated perfused rat kidney model. Methods and Results: Kidneys were isolated and perfused with modified Krebs-Henseleit. Perfusate and urine samples were obtained at baseline and every 10 minutes over 150 minutes following addition to the perfusate of challenges with or without treatments...
April 24, 2017: Infection and Immunity
https://www.readbyqxmd.com/read/28438904/loss-of-resistance-to-angiotensin-ii-induced-hypertension-in-the-jackson-laboratory-recombination-activating-gene-null-mouse-on-the-c57bl-6j-background
#8
Hong Ji, Amrita V Pai, Crystal A West, Xie Wu, Robert C Speth, Kathryn Sandberg
Resistance to angiotensin II (Ang II)-induced hypertension in T-cell-deficient male mice with a targeted mutation in the recombination-activating gene-1 (Rag1) on the C57BL/6J background (B6.Rag1(-)(/-) -M), which was reported by 5 independent laboratories including ours before 2015, has been lost. In mice purchased from Jackson Laboratory in 2015 and 2016, the time course and magnitude increase in mean arterial pressure induced by 2 weeks of Ang II infusion at 490 ng/kg per minute was identical between B6...
April 24, 2017: Hypertension
https://www.readbyqxmd.com/read/28438765/longitudinal-characterization-of-renal-proximal-tubular-markers-in-normotensive-and-preeclamptic-pregnancies
#9
Elisabeth Codsi, Vesna D Garovic, Maria L Gonzalez-Suarez, Natasa Milic, Kristi S Borowski, Carl H Rose, Norman P Davies, Kianoush Kashani, John C Lieske, Wendy M White
Glomerular damage is common in preeclampsia (PE), but the extent and etiology of tubular injury are not well understood. The aim of this study was to evaluate tubular injury in patients with PE and to assess whether it predates clinical disease. We performed a prospective cohort study of 315 pregnant women who provided urine samples at the end of the second trimester and at delivery. This analysis included women who developed PE (n=15), gestational hypertension (GH) (n=14), and normotensive controls (NC) (n=44)...
April 24, 2017: American Journal of Physiology. Regulatory, Integrative and Comparative Physiology
https://www.readbyqxmd.com/read/28438490/acute-nanoparticle-exposure-to-vocal-folds-a-laboratory-study
#10
Xinxin Liu, Tanaya Walimbe, William Pierre Schrock, Wei Zheng, M Preeti Sivasankar
OBJECTIVES: Airway exposure to nanoparticles is common in occupational settings. Inhaled nanoparticles have toxic effects on respiratory tissue. Vocal folds are also at direct risk from inhaled nanoparticles. This study investigated the effects of single-walled carbon nanotubes (SWCNT), a type of nanoparticle, on vocal fold epithelium and fibroblasts. These cell types were selected for study as the epithelium is the outer layer of the vocal folds and fibroblasts are the most common cell type in connective tissue underlying the epithelium...
April 21, 2017: Journal of Voice: Official Journal of the Voice Foundation
https://www.readbyqxmd.com/read/28437016/electromechanical-nanogenerator-cell-interaction-modulates-cell-activity
#11
Gonzalo Murillo, Andreu Blanquer, Carolina Vargas-Estevez, Lleonard Barrios, Elena Ibáñez, Carme Nogués, Jaume Esteve
Noninvasive methods for in situ electrical stimulation of human cells open new frontiers to future bioelectronic therapies, where controlled electrical impulses could replace the use of chemical drugs for disease treatment. Here, this study demonstrates that the interaction of living cells with piezoelectric nanogenerators (NGs) induces a local electric field that self-stimulates and modulates their cell activity, without applying an additional chemical or physical external stimulation. When cells are cultured on top of the NGs, based on 2D ZnO nanosheets, the electromechanical NG-cell interactions stimulate the motility of macrophages and trigger the opening of ion channels present in the plasma membrane of osteoblast-like cells (Saos-2) inducing intracellular calcium transients...
April 24, 2017: Advanced Materials
https://www.readbyqxmd.com/read/28435079/polyurethane-acrylates-as-effective-substrates-for-sustained-in-vitro-culture-of-human-myotubes
#12
Yosephine Andriani, Jason Chua Min-Wen, Benjamin Chua Yan-Jiang, Phang In Yee, Ng Shyh-Chang, Tan Wui Siew
Muscular disease has debilitating effects with severe damage leading to death. Our knowledge of muscle biology, disease and treatment is largely derived from non human cell models, even though non human cells are known to differ from human cells in their biochemical responses. Attempts to develop highly sought after in vitro human cell models have been plagued by early cell delamination and difficulties in achieving human myotube culture in vitro. In this work, we developed polyurethane acrylate (PUA) materials to support long term in vitro culture of human skeletal muscle tissue...
April 20, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28434258/protective-effects-of-casein-derived-peptide-vlpvpqk-against-hydrogen-peroxide-induced-dysfunction-and-cellular-oxidative-damage-in-rat-osteoblastic-cells
#13
S B Mada, S Reddi, N Kumar, S Kapila, R Kapila
Oxidative stress inhibits osteoblast differentiation and function that lead to the development of osteoporosis. Casein-derived peptide VLPVPQK (PEP), a potent antioxidant, was isolated from β-casein of buffalo milk. We used an in vitro oxidative stress model induced by hydrogen peroxide (H2O2) in rat osteoblastic cells to investigate the protective effects of PEP against H2O2-induced dysfunction and oxidative damage. Cells were pretreated with PEP (50-200 ng/mL) for 2, 7 or 21 days followed by 0.3 mM H2O2 treatment for 24 h and then markers of osteogenic development, oxidative damage and apoptosis were examined...
January 1, 2017: Human & Experimental Toxicology
https://www.readbyqxmd.com/read/28433068/the-role-of-interleukin-1%C3%AE-and-extracellular-signal-regulated-kinase-1-2-in-glucose-stimulated-insulin-secretion
#14
Ben Niu, Heng Su, Xue-Shan Xia, Qiu He, Yuan-Ming Xue, Xin-Ming Yan
Glucose-stimulated insulin secretion (GSIS) is one of the important physiological characteristics of islet β cells, and extracellular-regulated protein kinase 1/2 (ERK1/2) is an important member of the mitogen-activated protein kinase family that regulates this process. The inflammatory cytokine interleukin (IL)-1β can inhibit the insulin secretion of pancreatic β cells, but the exact mechanism is unclear. This study was designed to investigate the inhibitory effect of IL-1β on GSIS in βTC-6 cells and its relation with the ERK1/2 signal transduction pathway...
May 2017: Kaohsiung Journal of Medical Sciences
https://www.readbyqxmd.com/read/28432157/effect-of-interleukin-6-inhibition-on-coronary-microvascular-and-endothelial-function-in-myocardial-infarction
#15
Espen Holte, Ola Kleveland, Thor Ueland, Gabor Kunszt, Marte Bratlie, Kaspar Broch, Annika E Michelsen, Bjørn Bendz, Brage H Amundsen, Svend Aakhus, Jan Kristian Damås, Lars Gullestad, Pål Aukrust, Rune Wiseth
OBJECTIVE: Interleukin-6 (IL-6) is a driver of inflammation and associated endothelial cell activation in acute coronary syndromes. We evaluated the effect of the IL-6 receptor antagonist tocilizumab on coronary microvascular function and endothelial dysfunction measured by coronary flow reserve (CFR) and markers of endothelial cell activation in patients with non-ST-elevation myocardial infarction (NSTEMI). METHODS: This substudy was part of a two-centre, double-blind, randomised, placebo-controlled trial evaluating the effect of a single dose of tocilizumab in NSTEMI...
April 21, 2017: Heart: Official Journal of the British Cardiac Society
https://www.readbyqxmd.com/read/28431037/gestational-diabetes-alters-functions-in-offspring-s-umbilical-cord-cells-with-implications-for-cardiovascular-health
#16
Ajith Isaac Amrithraj, Anjaneyulu Kodali, Linh Nguyen, Adrian Kee Keong Teo, Cheng Wei Chang, Neerja Karnani, Kai Lyn Ng, Peter D Gluckman, Yap Seng Chong, Walter Stünkel
As non-communicable diseases such as type 2 diabetes mellitus have their roots in prenatal development and conditions such as maternal gestational diabetes (GDM), we aimed to test this hypothesis in primary cells derived from the offspring of GDM mothers compared to control subjects. We have assessed primary umbilical cord derived cells such as human umbilical vein endothelial cells (HUVECs) and Wharton's jelly derived mesenchymal stem cells (WJMSCs) from both, the offspring of GDM and non-GDM mothers. We have compared the primary isolates in cell based assays measuring proliferation, mitochondrial oxygen consumption, and the ability to support blood vessel growth...
April 19, 2017: Endocrinology
https://www.readbyqxmd.com/read/28429777/interleukin-6-increases-the-expression-and-activity-of-insulin-degrading-enzyme
#17
Mirian A Kurauti, José M Costa-Júnior, Sandra M Ferreira, Gustavo J Santos, Carlos H G Sponton, Everardo M Carneiro, Guilherme D Telles, Mara P T Chacon-Mikahil, Cláudia R Cavaglieri, Luiz F Rezende, Antonio C Boschero
Impairment of the insulin-degrading enzyme (IDE) is associated with obesity and type 2 diabetes mellitus (T2DM). Here, we used 4-mo-old male C57BL/6 interleukin-6 (IL-6) knockout mice (KO) to investigate the role of this cytokine on IDE expression and activity. IL-6 KO mice displayed lower insulin clearance in the liver and skeletal muscle, compared with wild type (WT), due to reduced IDE expression and activity. We also observed that after 3-h incubation, IL-6, 50 and 100 ng ml(-1), increased the expression of IDE in HEPG2 and C2C12 cells, respectively...
April 21, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28427421/cerebrospinal-fluid-csf-biomarkers-of-iron-status-are-associated-with-csf-viral-load-antiretroviral-therapy-and-demographic-factors-in-hiv-infected-adults
#18
Stephanie M Patton, Quan Wang, Todd Hulgan, James R Connor, Peilin Jia, Zhongming Zhao, Scott L Letendre, Ronald J Ellis, William S Bush, David C Samuels, Donald R Franklin, Harpreet Kaur, Jennifer Iudicello, Igor Grant, Asha R Kallianpur
BACKGROUND: HIV-associated neurocognitive disorder (HAND) remains common, despite antiretroviral therapy (ART). HIV dysregulates iron metabolism, but cerebrospinal fluid (CSF) levels of iron and iron-transport proteins in HIV-infected (HIV+) persons are largely unknown. The objectives of this study were to characterize CSF iron-related biomarkers in HIV+ adults and explore their relationships to known predictors of HAND. METHODS: We quantified total iron, transferrin and heavy-chain (H)-ferritin by immunoassay in CSF sampled by lumbar puncture in 403 HIV+ participants in a multi-center, observational study and evaluated biomarker associations with demographic and HIV-related correlates of HAND [e...
April 21, 2017: Fluids and Barriers of the CNS
https://www.readbyqxmd.com/read/28427157/the-anti-tumor-efficacy-of-3c23k-a-glyco-engineered-humanized-anti-misrii-antibody-in-an-ovarian-cancer-model-is-mainly-mediated-by-engagement-of-immune-effector-cells
#19
Pauline Estupina, Alexandre Fontayne, Jean-Marc Barret, Nathalie Kersual, Olivier Dubreuil, Marion Le Blay, Alexandre Pichard, Marta Jarlier, Martine Pugnière, Maëva Chauvin, Thierry Chardès, Jean-Pierre Pouget, Emmanuel Deshayes, Alexis Rossignol, Toufik Abache, Christophe de Romeuf, Aurélie Terrier, Lucie Verhaeghe, Christine Gaucher, Jean-François Prost, André Pèlegrin, Isabelle Navarro-Teulon
Ovarian cancer is the leading cause of death in women with gynecological cancers and despite recent advances, new and more efficient therapies are crucially needed. Müllerian Inhibiting Substance type II Receptor (MISRII, also named AMHRII) is expressed in most ovarian cancer subtypes and is a novel potential target for ovarian cancer immunotherapy. We previously developed and tested 12G4, the first murine monoclonal antibody (MAb) against human MISRII. Here, we report the humanization, affinity maturation and glyco-engineering steps of 12G4 to generate the Fc-optimized 3C23K MAb, and the evaluation of its in vivo anti-tumor activity...
February 24, 2017: Oncotarget
https://www.readbyqxmd.com/read/28427133/-effects-of-application-of-vancomycin-in-the-early-stage-of-patients-with-extremely-severe-burn
#20
Z Zhu, G W Cao, J J Bao, Z H Hu, Z Shen, H Tao, B Cao, F Xu
Objective: To evaluate the effects of application of vancomycin in the early stage of patients with extremely severe burn, in order to provide reference to drug for anti-infection treatment in the early stage of patients with extremely severe burn. Methods: Data of 15 patients of Kunshan explosion on August 2nd, 2014, admitted to the Department of Intensive Care in our hospital were retrospectively analyzed. The clinical efficacy of continuously intravenous dripping of vancomycin (combined with imipenem) in the early stage of burns (before and on post burn day 14) was analyzed...
April 20, 2017: Zhonghua Shao Shang za Zhi, Zhonghua Shaoshang Zazhi, Chinese Journal of Burns
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