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https://www.readbyqxmd.com/read/29341904/a-rapid-and-simple-lc-ms-ms-method-for-personalized-busulfan-dosing-in-pediatric-patients-undergoing-hematological-stem-cell-transplantation-hsct
#1
Yi Xiao, Xiaohan Li, Xiaowei Fu
BACKGROUND: Busulfan is commonly used as a conditioning regimen before hematological stem cell transplantation (HSCT). There is a big inter-individual variability in busulfan exposure and the narrow therapeutic index, especially in pediatric population. Therefore, to achieve therapeutic efficacy and safety concurrently, personalized busulfan dosing, guided by pharmacokinetic study with serial plasma samples, is needed a few hours afterwards. METHODS: We used liquid-chromatography-mass spectrometry (HPLC-MS/MS) to develop, validate, and implementation of a fast, sensitive, and accurate method for busulfan measurement...
January 13, 2018: Clinica Chimica Acta; International Journal of Clinical Chemistry
https://www.readbyqxmd.com/read/29339342/transgenerational-impairments-of-reproduction-and-development-of-the-marine-invertebrate-crepidula-onyx-resulted-from-long-term-dietary-exposure-of-2-2-4-4-tetrabromodiphenyl-ether-bde-47
#2
Beverly H K Po, Jill M Y Chiu
Polybrominated diphenyl ethers have become ubiquitous in the environment and elevated concentrations have often been found in marine organisms. Using the gastropod Crepidula onyx as a study model, this multigenerational study sets out to test the hypotheses that 1) parental dietary exposure to environmentally realistic levels of 2,2',4,4'-tetrabrominated diphenyl ether (BDE-47) would lead to transgenerational impairments on fitness traits of marine invertebrates, and 2) the organisms might develop adaptation/acclimation after exposure for one or more generations...
January 12, 2018: Environmental Pollution
https://www.readbyqxmd.com/read/29339271/early-increase-in-complement-terminal-pathway-activation-marker-sc5b-9-is-predictive-for-the-development-of-thrombotic-microangiopathy-after-stem-cell-transplantation
#3
Orsolya Horváth, Krisztián Kállay, Dorottya Csuka, Blanka Mező, György Sinkovits, Csaba Kassa, Anita Stréhn, Katalin Csordás, János Sinkó, Zoltán Prohászka, Gergely Kriván
Hematopoietic stem cell transplantation- (HSCT-) associated thrombotic microangiopathy (TA-TMA) is a multifactorial complication, and its prediction is largely unresolved.Our aim was to analyze changes of complement profile after HSCT in order to identify potential markers of TA-TMA development. Thirty- three consecutive pediatric patients (9.6 ± 4.4 years old) were included in this study who underwent allogeneic HSCT due to malignant (n=17) or non malignant (n=16) indications. Graft versus host disease (GVHD) was diagnosed using Glucksberg criteria, viral reactivation was monitored, five different TA-TMA diagnostic criteria were applied and all important clinical and laboratory parameters of TA-TMA activity were registered...
January 12, 2018: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/29334548/plasma-and-intracellular-pharmacokinetics-of-tenofovir-in-patients-switched-from-tenofovir-disoproxil-fumarate-to-tenofovir-alafenamide
#4
Anthony T Podany, Sara H Bares, Joshua Havens, Ravi Dyavar Shetty, Jennifer O'neill, Sarah Lee, Courtney V Fletcher, Susan Swindells, Kimberly K Scarsi
OBJECTIVES: The aim of the study was to compare the intra-individual plasma and intracellular peripheral blood mononuclear cell (PBMC) pharmacokinetics (PK) of tenofovir (TFV) and its intracellular metabolite, tenofovir-diphosphate (TFV-DP) in patients switched from a fixed dose combination (FDC) tablet of tenofovir disoproxil fumarate (TDF)/ emtricitabine (FTC)/ elvitegravir (EVG)/ cobicistat (COBI) to a FDC containing tenofovir alafenamide (TAF)/FTC/EVG/COBI. DESIGN: A single arm, prospective, non-randomized, cross-over, PK study in patients receiving a TDF-containing regimen (TDF 300 mg/ FTC 200 mg/ EVG 150 mg/ COBI 150 mg) switched to a TAF-containing FDC regimen (TAF 10 mg/ FTC 200 mg/ EVG 150 mg/ COBI 150 mg)...
January 13, 2018: AIDS
https://www.readbyqxmd.com/read/29334210/combined-effect-of-retinoic-acid-and-basic-fibroblast-growth-factor-on-maturation-of-mouse-oocyte-and-subsequent-fertilization-and-development
#5
Morteza Abouzaripour, Fardin Fathi, Erfan Daneshi, Keywan Mortezaee, Mohammad Jafar Rezaie, Mahdad Abdi
Background: In this experimental study, germinal vesicle (GV) oocytes obtained from two-months-old NMRI mice were randomly divided into control, sham and three experimental groups. The basic culture medium was α-MEM supplemented with 10% fetal bovine serum (FBS), 50 mg/l streptomycin, 60 mg/l penicillin and 10 ng/ ml epidermal growth factors. Each of the experimental groups received one of the following treatments: RA (2 μM), bFGF (20 ng/ml) or combination of RA and bFGF with the indicated concentrations...
April 2018: International Journal of Fertility & Sterility
https://www.readbyqxmd.com/read/29332451/psca-rs1045531-polymorphism-and-the-risk-of-prostate-cancer-in-a-chinese-population-undergoing-prostate-biopsy
#6
Xuefeng Zhang, Qin Hu, Ye Chen, Miao Li, Huming Yin, Chenchao Zhou, Gang Li, Jianquan Hou
BACKGROUND AND PURPOSE: This study explored the association between a single-nucleotide polymorphism of prostate stem cell antigen and prostate cancer in Chinese patients undergoing prostate biopsy. MATERIALS AND METHODS: DNA from 416 patients undergoing prostate biopsy was typed for the prostate stem cell antigen rs1045531 single-nucleotide polymorphism. The frequency of the rs1045531 polymorphism in patients with prostate cancer and in patients with benign prostatic hyperplasia was compared...
December 2017: Technology in Cancer Research & Treatment
https://www.readbyqxmd.com/read/29331857/baicalin-alleviates-il-1%C3%AE-induced-inflammatory-injury-via-down-regulating-mir-126-in-chondrocytes
#7
Xirui Yang, Qi Zhang, Zhaomeng Gao, Chunyan Yu, Lei Zhang
Baicalin is a flavonoid extracted from Scutellaria baicalensis Georgi, with anti-inflammatory and anti-apoptotic activities. The objective of this study was to explore the effect and mechanism of baicalin on chondrocyte inflammatory response in OA. Different concentrations of IL-1β (0, 0.1, 2, 5 and 10 ng/mL) were used to simulate inflammatory injury in CHON-001 cells. The expression of miR-126 was altered by transfection with miR-126 mimic. Thereafter, cells were treated with baicalin, and cell viability, apoptosis, the expressions of apoptosis-related protein and pro-inflammatory factors were respectively detected using CCK-8 assay, flow cytometry, qRT-PCR and western blot analysis...
January 11, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29331377/%C3%AE-cellulin-promotes-the-proliferation-of-corneal-epithelial-stem-cells-through-the-phosphorylation-of-erk1-2
#8
Won-Yong Jeong, Hye-Young Yoo, Chan-Wha Kim
The proliferation of corneal epithelial stem cells (CESCs) is a very important process in the recovery of corneal wounds. Recent studies have shown that β-cellulin (BC) is effective in the repair of other tissues. However, its mechanism of action in corneal wound healing is not yet clear. The purpose of this study was to investigate how BC accelerates wound healing of the cornea. Here, we confirmed that the proliferation of CESCs was induced at a specific concentration (0.2, 2 and 20 ng/mL) by treatment with BC...
January 10, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29330220/regulation-of-drug-metabolism-by-the-interplay-of-inflammatory-signaling-steatosis-and-xeno-sensing-receptors-in-heparg-cells
#9
Norman Tanner, Lisa Kubik, Claudia Luckert, Maria Thomas, Ute Hofmann, Ulrich M Zanger, Linda Bohmert, Alfonso Lampen, Albert Braeuning
Non-alcoholic fatty liver disease (NAFLD), characterized by triglyceride deposition in hepatocytes due to imbalanced lipid homeostasis, is of increasing concern in Western countries, with progression to non-alcoholic steatohepatitis (NASH), liver fibrosis, and cirrhosis. Previous studies suggest a complex, mutual influence of hepatic fat accumulation, NASH-related inflammatory mediators, and drug-sensing receptors regulating xenobiotic metabolism. Here, we investigated the suitability of human HepaRG hepatocarcinoma cells as a model for NAFLD and NASH...
January 12, 2018: Drug Metabolism and Disposition: the Biological Fate of Chemicals
https://www.readbyqxmd.com/read/29328397/aberrant-expression-of-il-23-il-23r-in-patients-with-breast-cancer-and-its-clinical-significance
#10
Shuhai Sheng, Jinji Zhang, Jianzhong Ai, Xueli Hao, Ruishen Luan
Breast cancer tissues and adjacent tissues were collected from 32 patients who were treated in The Third Hospital of Chengde City. Reverse transcription‑quantitative polymerase chain reaction results demonstrated that, compared with the adjacent tissues, interleukin (IL)‑23/IL‑23 receptor (R) gene expression levels were notably higher in breast cancer tissues. Furthermore, IL‑23 and IL‑23R expression levels were positively correlated with patients' tumor size, TNM stage and metastasis. Recombinant human (rh) IL‑23 (10 ng/ml) was used for the stimulation of the MCF‑7 cell line...
January 12, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29325591/correction-to-rp58-and-p27kip1-coordinate-cell-cycle-exit-and-neuronal-migration-within-the-embryonic-mouse-cerebral-cortex
#11
Olivier Clément, Isabel Anne Hemming, Ivan Enghian Gladwyn-Ng, Zhengdong Qu, Shan Shan Li, Michael Piper, Julian Ik-Tsen Heng
After publication of the original article [1] it was realised that there were errors in figures 2a,b,f,g, which arose as a result of preparing figures from data collected and analysed at the same time as the work reported in [2] (Supplementary Figure 1 of [2]). An updated Fig. 2 is included with this Correction.
January 11, 2018: Neural Development
https://www.readbyqxmd.com/read/29325416/igy-reduces-afb1-induced-cytotoxicity-cellular-dysfunction-and-genotoxicity-in-human-l-02-hepatocytes-and-swan-71-trophoblasts
#12
Taotao Qiu, Xing Shen, Zhen Tian, Riming Huang, Xiangmei Li, Juan Wang, Rong Wang, Yuanming Sun, Yiguo Jiang, Huidong Zhang, Hongtao Lei
Aflatoxin B1 (AFB1) causes hepatotoxic, genotoxic, and immuno¬toxic effects in a variety of species. Although various neutralizing agents of AFB1 toxicity have been studied, the egg yolk immunoglobulins (IgY) detoxification of small molecular toxins and the mechanisms underlying such effects have not yet been reported. In this investigation, anti-AFB1 IgY against AFB1 was successfully raised, and a competitive indirect enzyme-linked immunosorbent assay was established with a sensitive half-maximal inhibitory concentration (IC50, 2...
January 11, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29325368/-the-effects-and-related-mechanism-of-salvianolate-on-rats-with-myocardial-ischemia-reperfusion-injury
#13
R C Yue, X L Yang, R Y Zhang, S Liu, J Liu, J Zeng, H Liang, W Wang, H X Hu, C Y Zeng
Objective: To investigate the effect and related mechanism of salvianolate on myocardial ischemia-reperfusion (I/R) injury in rats. Methods: Thirty-six adult Sprague-Dawley rats were divided into three groups (n=12 each) using random number table method: control group (coronary artery was not ligated) , I/R group (myocardial I/R model was established by ligation and opening of left anterior descending coronary artery) , and salvianolate+I/R group (5 mg/kg of salvianolate was injected through the tail vein at the time of reperfusion) ...
December 24, 2017: Zhonghua Xin Xue Guan Bing za Zhi
https://www.readbyqxmd.com/read/29325127/inhibiting-trophoblast-par-1-overexpression-suppresses-sflt-1-induced-anti-angiogenesis-and-abnormal-vascular-remodeling-a-possible-therapeutic-approach-for-preeclampsia
#14
Yin Zhao, YanFang Zheng, XiaoXia Liu, QingQing Luo, Di Wu, XiaoPing Liu, Li Zou
STUDY QUESTION: Is it possible to improve vascular remodeling by inhibiting the excessive expression of protease activated receptor 1 (PAR-1) in trophoblast of abnormal placenta? SUMMARY ANSWER: Inhibition of trophoblast PAR-1 overexpression may promote placental angiogenesis and vascular remodeling, offering an alternative therapeutic approach for preeclampsia. WHAT IS KNOWN ALREADY: PAR-1 is high-affinity receptor of thrombin. Thrombin increases sFlt-1 secretion in trophoblast via the activation of PAR-1...
January 9, 2018: Molecular Human Reproduction
https://www.readbyqxmd.com/read/29324635/diagnostic-value-of-serum-urokinase-type-plasminogen-activator-receptor-in-children-with-acute-appendicitis
#15
Mustafa Onur Oztan, Aysegul Aksoy Gokmen, Fatma Demet Arslan, Ebru Cakir, Ali Sayan, Elif Abay, Selçuk Kaya, Gokhan Koyluoglu
OBJECTIVES: Acute appendicitis (AA) is the most common surgical emergency in children. The accurate and timely diagnosis of AA in children can be challenging, and delayed diagnosis rates have been reported to range from 5.9% to 27.6%. Although combining clinical history and repeated physical examination with laboratory tests and radiographic imaging modalities help reach the diagnosis, novel biomarkers can support the surgeons' decision as well. The aims of this study were to evaluate a new plasma marker, urokinase-type plasminogen activator receptor (uPAR), to improve diagnostic accuracy in AA patients, and to determine a cutoff value of uPAR, which can safely include/exclude the diagnosis of AA...
January 11, 2018: Pediatric Emergency Care
https://www.readbyqxmd.com/read/29323531/an-efficient-gas-chromatography-mass-spectrometry-approach-for-the-simultaneous-analysis-of-deoxynivalenol-and-its-bacterial-metabolites-3-keto-don-and-3-epi-don
#16
Kaijie Tang, Huaizhi Liu, Xiu-Zhen Li, Yousef I Hassan, Suqin Shao, Ting Zhou
Deoxynivalenol (DON) is one of the major toxic secondary metabolites produced by Fusarium fungi in cereal grains. Among the many promising strategies of DON detoxification are the microbial and enzymatic ones, which transform DON to nontoxic DON metabolites. Thus, proper analytical methods are needed for those DON metabolites. In this study, a robust gas chromatography-mass spectrometry (GC-MS) procedure was developed and validated for the simultaneous analysis of DON and two of its bacterial metabolites, 3-keto-DON and 3- epi-DON...
January 11, 2018: Journal of Food Protection
https://www.readbyqxmd.com/read/29323215/green-tea-extract-attenuates-lps-induced-retinal-inflammation-in-rats
#17
Jia Lin Ren, Qiu Xiao Yu, Wei Cheng Liang, Pui Ying Leung, Tsz Kin Ng, Wai Kit Chu, Chi Pui Pang, Sun On Chan
Inflammation is in a wide spectrum of retinal diseases, causing irreversible blindness and visual impairment. We have previously demonstrated that Green Tea Extract (GTE) is a potent anti-inflammatory agent for anterior uveitis. Here we investigated the anti-inflammatory effect of GTE on lipopolysaccharides (LPS)-induced retinal inflammation in rats and explored the underlying mechanism. Adult rats were injected with LPS and GTE was administered intra-gastrically at 2, 8, 26 and 32 hours post-injection. Staining of whole-mount retina showed that the number of activated microglia cells was significantly increased at 48 hours post-injection, which was suppressed after GTE treatment in a dose-dependent manner...
January 11, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321582/serum-podocalyxin-levels-correlate-with-carotid-intima-media-thickness-implicating-its-role-as-a-novel-biomarker-for-atherosclerosis
#18
Mayumi Shoji, Minoru Takemoto, Kazuki Kobayashi, Toshihiro Shoji, Satoka Mori, Jun-Ichi Sagara, Hiroyuki Kurosawa, Yoshiaki Hirayama, Kenichi Sakamoto, Takahiro Ishikawa, Masaya Koshizaka, Yoshiro Maezawa, Koutaro Yokote
Podocalyxin is a cell surface sialomucin, which is expressed in not only glomerular podocytes but also vascular endothelial cells. Urinary podocalyxin is used as a marker for glomerular disease. However, there are no reports describing serum podocalyxin (s-Podxl) levels. Therefore, the association between s-Podxl levels and clinical parameters were examined with 52 patients. s-Podxl level was evaluated using enzyme-linked immunosorbent assay. The median s-Podxl level was 14.2 ng/dL (interquartile range: 10...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321510/a-model-of-human-lung-fibrogenesis-for-the-assessment-of-anti-fibrotic-strategies-in-idiopathic-pulmonary-fibrosis
#19
Katy M Roach, Amanda Sutcliffe, Laura Matthews, Gill Elliott, Chris Newby, Yassine Amrani, Peter Bradding
Idiopathic pulmonary fibrosis (IPF) is a progressive interstitial lung disease with limited therapeutic options. KCa3.1 ion channels play a critical role in TGFβ1-dependent pro-fibrotic responses in human lung myofibroblasts. We aimed to develop a human lung parenchymal model of fibrogenesis and test the efficacy of the selective KCa3.1 blocker senicapoc. 2 mm3 pieces of human lung parenchyma were cultured for 7 days in DMEM ± TGFβ1 (10 ng/ml) and pro-fibrotic pathways examined by RT-PCR, immunohistochemistry and collagen secretion...
January 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29321372/chronic-skin-inflammation-accelerates-macrophage-cholesterol-crystal-formation-and-atherosclerosis
#20
Yvonne Baumer, Qimin Ng, Gregory E Sanda, Amit K Dey, Heather L Teague, Alexander V Sorokin, Pradeep K Dagur, Joanna I Silverman, Charlotte L Harrington, Justin A Rodante, Shawn M Rose, Nevin J Varghese, Agastya D Belur, Aditya Goyal, Joel M Gelfand, Danielle A Springer, Christopher Ke Bleck, Crystal L Thomas, Zu-Xi Yu, Mårten Cg Winge, Howard S Kruth, M Peter Marinkovich, Aditya A Joshi, Martin P Playford, Nehal N Mehta
Inflammation is critical to atherogenesis. Psoriasis is a chronic inflammatory skin disease that accelerates atherosclerosis in humans and provides a compelling model to understand potential pathways linking these diseases. A murine model capturing the vascular and metabolic diseases in psoriasis would accelerate our understanding and provide a platform to test emerging therapies. We aimed to characterize a new murine model of skin inflammation (Rac1V12) from a cardiovascular standpoint to identify novel atherosclerotic signaling pathways modulated in chronic skin inflammation...
January 11, 2018: JCI Insight
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