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https://www.readbyqxmd.com/read/28732084/increased-parb-level-affects-expression-of-stress-response-adaptation-and-virulence-operons-and-potentiates-repression-of-promoters-adjacent-to-the-high-affinity-binding-sites-pars3-and-pars4-in-pseudomonas-aeruginosa
#1
Adam Kawalek, Krzysztof Glabski, Aneta Agnieszka Bartosik, Anna Fogtman, Grazyna Jagura-Burdzy
Similarly to its homologs in other bacteria, Pseudomonas aeruginosa partitioning protein ParB facilitates segregation of newly replicated chromosomes. Lack of ParB is not lethal but results in increased frequency of anucleate cells production, longer division time, cell elongation, altered colony morphology and defective swarming and swimming motility. Unlike in other bacteria, inactivation of parB leads to major changes of the transcriptome, suggesting that, directly or indirectly, ParB plays a role in regulation of gene expression in this organism...
2017: PloS One
https://www.readbyqxmd.com/read/28731936/case-series-keratolimbal-allograft-as-a-patch-graft-for-glaucoma-drainage-devices
#2
Sarah F Ahmed, Mason Schmutz, Sameh Mosaed
PURPOSE: Tube exposure remains one of the most common complications after glaucoma drainage device (GDD) implantation, despite various types of patch grafts available today. We present a 4 patient case series following the effectivity of the keratolimbal allograft (KLAL) as a patch graft for cases of tube exposure. Given its inherent population of stem cells, our hypothesis was that this highly replicative, biological tissue would provide an adequate means of glaucoma tube coverage. The subset of patients chosen for the KLAL patch graft all had a history of abnormally scarred conjunctiva or thin sclera...
July 20, 2017: Journal of Glaucoma
https://www.readbyqxmd.com/read/28731694/the-foliar-desiccators-glyphosate-carfentrazone-and-paraquat-affect-the-technological-and-chemical-properties-of-cowpea-grains
#3
Igor da Silva Lindemann, Gustavo Heinrich Lang, Jessica Fernanda Hoffmann, Cesar Valmor Rombaldi, Mauricio de Oliveira, Moacir Cardoso Elias, Nathan Levien Vanier
The effects of the use of glyphosate (GLY), glyphosate plus carfentrazone (GLY/CAR), and paraquat (PAR) as plant desiccators on the technological and chemical properties of cowpea grains were investigated. All studied desiccants provided lower cooking time to freshly harvested cowpea. However, the coat color of PAR- and GLY/CAR-treated cowpea was reddish than control treatment. Principal component analysis (PCA) from liquid chromatography-mass spectrometry (LC-MS) data sets showed a clear distinction among cowpea from the different treatments...
July 21, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28731058/multicenter-study-of-pars-plana-vitrectomy-for-optic-disc-pit-maculopathy-macpit-study
#4
R Avci, Z Kapran, Ş Ozdek, M Y Teke, O Oz, D Guven, S Yilmaz, B Kaderli, A H Durukan, G Sobaci, Y B Unver, L Akduman, S Kaynak, I Dogan, U U Inan
PurposeTo evaluate surgical intervention with pars plana vitrectomy (PPV) for correction of optic disc pit maculopathy (ODP-M).Patients and methodsRetrospective chart review from 13 centres of 51 eyes of 50 patients with ODP-M who underwent PPV between 2002-2014. Anatomic and final best-corrected visual acuity (BCVA) outcomes were evaluated for all cases with different adjuvant techniques.ResultsThere were 23 males and 27 females with median age 25.5 (6-68) years. Preoperative median foveal thickness was 694...
July 21, 2017: Eye
https://www.readbyqxmd.com/read/28729676/proteomic-profiling-of-the-weed-feverfew-a-neglected-pollen-allergen-source
#5
Isabel Pablos, Stephanie Eichhorn, Peter Briza, Claudia Asam, Ulrike Gartner, Martin Wolf, Christof Ebner, Barbara Bohle, Naveen Arora, Stefan Vieths, Fatima Ferreira, Gabriele Gadermaier
Feverfew (Parthenium hysterophorus), an invasive weed from the Asteraceae family, has been reported as allergen source. Despite its relevance, knowledge of allergens is restricted to a partial sequence of a hydroxyproline-rich glycoprotein. We aimed to obtain the entire sequence for recombinant production and characterize feverfew pollen using proteomics and immunological assays. Par h 1, a defensin-proline fusion allergen was obtained by cDNA cloning and recombinantly produced in E. coli. Using two complementary proteomic strategies, a total of 258 proteins were identified in feverfew pollen among those 47 proteins belonging to allergenic families...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28729635/superior-non-specific-motor-learning-in-the-blind
#6
Florence Morin-Parent, Louis de Beaumont, Hugo Théoret, Jean-Francois Lepage
It is well established that blindness induces changes in cerebral function and structure, namely affecting the somatomotor regions. However, the behavioural significance of these changes on the motor system, and on motor learning in particular, remains elusive. In this study, we used a modified version of the serial reaction time task (SRTT) with auditory cues to assess sequence specific and non-specific motor learning in blind adults and sighted controls, and compare them with sighted controls performing the classic visual SRTT...
July 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28729109/n-o-211-d%C3%A3-pistage-et-prise-en-charge-de-la-vaginose-bact%C3%A3-rienne-pendant-la-grossesse
#7
Mark H Yudin, Deborah M Money
OBJECTIF: Analyser les résultats et fournir des recommandations au sujet du dépistage et de la prise en charge de la vaginose bactérienne pendant la grossesse. OPTIONS: Les options de pratique clinique prises en considération au moment de formuler la présente directive clinique. ISSUES: Parmi les issues évaluées, on trouve les taux d'efficacité et de guérison de l'antibiothérapie, et l'influence de la prise en charge de la vaginose bactérienne sur les taux d'issues de grossesse indésirables (tels que le travail et l'accouchement prétermes, et la rupture prématurée des membranes préterme)...
August 2017: Journal of Obstetrics and Gynaecology Canada: JOGC, Journal D'obstétrique et Gynécologie du Canada: JOGC
https://www.readbyqxmd.com/read/28729105/n-o-223-tenue-syst%C3%A3-matique-d%C3%A2-un-examen-%C3%A3-chographique-obst%C3%A3-trical-au-cours-du-deuxi%C3%A3-me-trimestre-contenu-d%C3%A2-un-examen-et-d%C3%A2-un-rapport-exhaustifs
#8
Yvonne Cargill, Lucie Morin
OBJECTIF: Analyser les avantages d'un examen échographique exhaustif au cours du deuxième trimestre et les conditions nécessaires à sa tenue, ainsi que la documentation requise. ISSUES: Un examen échographique exhaustif au cours du deuxième trimestre offre des renseignements au sujet du nombre de fœtus, de l'âge gestationnel, de l'emplacement du placenta et de l'anatomie fœtale et maternelle. RéSULTATS: Dans le cadre de la rédaction du présent document, le document « Practice Guideline for the Performance of Obstetric Ultrasound Examinations » du American Institute of Ultrasound in Medicine, le bulletin de pratique « Ultrasound in Pregnancy » du American College of Obstetricians and Gynaecologists et le rapport de groupe de travail « Ultrasound Screening » du Royal College of Obstetricians and Gynaecologists ont été analysés...
August 2017: Journal of Obstetrics and Gynaecology Canada: JOGC, Journal D'obstétrique et Gynécologie du Canada: JOGC
https://www.readbyqxmd.com/read/28729103/n-o-225-lignes-directrices-quant-%C3%A3-la-prise-en-charge-des-patientes-en-obst%C3%A3-trique-chez-lesquelles-la-pr%C3%A3-sence-du-syndrome-respiratoire-aigu-s%C3%A3-v%C3%A3-re-sras-est-soup%C3%A3-onn%C3%A3-e-ou-probable-et-des-nouveau-n%C3%A3-s-issus-de-ces-patientes
#9
Cynthia Maxwell, Alison McGeer, Kin Fan Young Tai, Mathew Sermer
OBJECTIF: Le présent document résume notre expérience limitée quant à la présence du SRAS pendant la grossesse et suggère des lignes directrices quant à sa prise en charge. ISSUES: Les exposés de cas issus d'Asie laissent entendre que les issues maternelles et fœtales sont aggravées par la présence du SRAS pendant la grossesse. RéSULTATS: Des recherches ont été menées dans Medline afin d'en tirer les articles pertinents publiés en anglais entre 2000 et 2007...
August 2017: Journal of Obstetrics and Gynaecology Canada: JOGC, Journal D'obstétrique et Gynécologie du Canada: JOGC
https://www.readbyqxmd.com/read/28728182/endophthalmitis-and-concurrent-or-delayed-onset-rhegmatogenous-retinal-detachment-managed-with-pars-plana-vitrectomy-intravitreal-antibiotics-and-silicone-oil
#10
Vivek Pravin Dave, Avinash Pathengay, Nidhi Relhan, Pranjali Sharma, Subhadra Jalali, Rajeev Reddy Pappuru, Mudit Tyagi, Raja Narayanan, Jay Chhablani, Taraprasad Das, Harry W Flynn
BACKGROUND AND OBJECTIVE: The current study describes the treatment outcomes in patients with endophthalmitis and concurrent or delayed-onset retinal detachment managed with pars plana vitrectomy, intravitreal antibiotics, and silicone oil. PATIENTS AND METHODS: In this noncomparative, retrospective case series, the medical records of patients diagnosed with endophthalmitis and retinal detachment from January 1991 through December 2014 at a tertiary eye care center in South India were reviewed...
July 1, 2017: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/28728160/structural-changes-of-the-macula-on-optical-coherence-tomography-after-vitrectomy-for-proliferative-diabetic-retinopathy
#11
Jong Chan Im, Jong Ho Kim, Dong Ho Park, Jae Pil Shin
PURPOSE: This study aimed to evaluate the relationship between macular structural changes and visual prognosis after pars plana vitrectomy (PPV) for proliferative diabetic retinopathy. METHODS: The study included 60 eyes that had undergone PPV. Macular optical coherence tomography (OCT) findings were classified into 5 groups preoperatively and 10 groups postoperatively. Best-corrected visual acuity (BCVA) were analyzed according to pre- and postoperative OCT. RESULTS: From the preoperative OCT, normal fovea with/without traction, normal fovea with preretinal hemorrhage, and tractional retinal detachment involving fovea showed an increase in BCVA after PPV (all p < 0...
July 21, 2017: Ophthalmologica. Journal International D'ophtalmologie
https://www.readbyqxmd.com/read/28727985/comparative-study-and-analytical-verification-of-pcr-methods-for-the-diagnosis-of-congenital-chagas-disease
#12
Carolina Inés Cura, Juan Carlos Ramírez, Marcelo Rodríguez, Constanza Lopez-Albízu, Lucía Irazu, Karenina Scollo, Sergio Sosa-Estani
Congenital infection is currently the first cause of new cases of Chagas disease in Argentina and nonendemic areas worldwide. Its diagnosis is of utmost importance to guarantee curative treatment. To improve such diagnosis, a transfer process of PCR tests to the national laboratory network has been initiated. We performed a comparative study of four PCR assays [two end-point PCR and two duplex real-time quantitative PCR (qPCR) procedures] to detect Trypanosoma cruzi DNA in blood samples. Because satellite DNA and kinetoplastid DNA qPCR methods have the best performance and the use of two different molecular targets for confirmatory purposes has been recommended, these methods selected to perform the transfer process and, in consequence, subjected to an analytical verification protocol based on international guidelines...
July 17, 2017: Journal of Molecular Diagnostics: JMD
https://www.readbyqxmd.com/read/28727723/analytical-sensitivity-and-specificity-of-a-loop-mediated-isothermal-amplification-lamp-kit-prototype-for-detection-of-trypanosoma-cruzi-dna-in-human-blood-samples
#13
Susana A Besuschio, Mónica Llano Murcia, Alejandro F Benatar, Severine Monnerat, Israel Cruz Mata, Albert Picado de Puig, María de Los Ángeles Curto, Yutaka Kubota, Diana P Wehrendt, Paula Pavia, Yasuyoshi Mori, Concepción Puerta, Joseph M Ndung'u, Alejandro G Schijman
This study aimed to assess analytical parameters of a prototype LAMP kit that was designed for detection of Trypanosoma cruzi DNA in human blood. The prototype is based on the amplification of the highly repetitive satellite sequence of T.cruzi in microtubes containing dried reagents on the inside of the caps. The reaction is carried out at 65°C during 40 minutes. Calcein allows direct detection of amplified products with the naked eye. Inclusivity and selectivity were tested in purified DNA from Trypanosoma cruzi stocks belonging to the six discrete typing units (DTUs), in DNA from other protozoan parasites and in human DNA...
July 20, 2017: PLoS Neglected Tropical Diseases
https://www.readbyqxmd.com/read/28724830/drug-resistant-coagulase-negative-staphylococcal-endophthalmitis-following-dexamethasone-intravitreal-implant
#14
Prabhushanker Mahalingam, Tasneem Tameem Topiwalla, Geetha Ganesan
A 60-year-old female came to our hospital with defective vision due to persistent diabetic macular edema and was treated with intravitreal ozurdex implant in the right eye. Three days later, the patient presented with diminution of vision, ocular pain, congestion, and hypopyon with no fundus view. B-scan ultrasonography showed vitritis, and diagnosis of endophthalmitis was made. Subsequently, 25-gauge pars plana vitrectomy was performed along with intravitreal vancomycin and amikacin and removal of implant...
July 2017: Indian Journal of Ophthalmology
https://www.readbyqxmd.com/read/28723849/comparative-analysis-of-retinal-reattachment-surgery-with-or-without-internal-limiting-membrane-peeling-to-prevent-postoperative-macular-pucker
#15
Matteo Forlini, Purva Date, Luisa Micelli Ferrari, Massimo Lorusso, Gabriella Lecce, Tommaso Verdina, Giovanni Neri, Caterina Benatti, Paolo Rossini, Adriana Bratu, Domenico DʼEliseo, Tommaso Micelli Ferrari, Gian Maria Cavallini
PURPOSE: To determine whether internal limiting membrane (ILM) peeling during pars plana vitrectomy for rhegmatogenous retinal detachment reduces the incidence of epiretinal membrane (ERM) formation. METHODS: In this retrospective study, preoperative, intraoperative, and postoperative data from all eyes undergoing pars plana vitrectomy for rhegmatogenous retinal detachment between January 2007 and December 2013 was analyzed. All cases with at least 1-year of follow-up were included...
July 18, 2017: Retina
https://www.readbyqxmd.com/read/28723847/blindness-related-to-presumed-retinal-toxicity-after-using-perfluorocarbon-liquid-during-vitreoretinal-surgery
#16
Silvia Méndez-Martínez, Pilar Calvo, Nelson Arturo Rodriguez-Marco, Fernando Faus, Emilio Abecia, Luis Pablo
PURPOSE: To describe the presumed retinal toxicity after using specific batches of perfluorocarbon liquid ALA OCTA (Alamedics, Dornstadt, Germany) in pars plana vitrectomy. METHODS: This is an observational retrospective consecutive case series analyses of patients operated on pars plana vitrectomy for retinal detachment or intraocular lens subluxation, using the 150141 or 200114 batches of perfluorocarbon liquid ALA OCTA as assistance during the surgery in a single center...
July 18, 2017: Retina
https://www.readbyqxmd.com/read/28721544/sleep-in-older-adults-and-in-subjects-with-dementia
#17
REVIEW
Helmut Frohnhofen, Jeanina Schlitzer, Nikolaus Netzer
The neuronal structures for the regulation of sleep and wakefulness are located in the brain. This complex network is vulnerable to numerous factors, most importantly neurodegenerative diseases and drugs. The macrostructure and microstructure of sleep change with age. These changes are more pronounced in subjects with dementia. Sleep disorders in subjects with dementia may be independent of dementia or caused by dementia. Furthermore, epidemiological studies reveal that sleep disorders per se may induce dementia by reduction of cerebral clearance of beta-amyloids...
July 18, 2017: Zeitschrift Für Gerontologie und Geriatrie
https://www.readbyqxmd.com/read/28720068/inhibitory-effect-of-par-4-combined-with-cisplatin-on-human-wilms-tumor-cells
#18
Jun Wang, Yunjie Li, Fangfang Ma, Huifeng Zhou, Rong Ding, Binbin Lu, Li Zou, Junxia Li, Rugang Lu
Wilms' tumor is associated with a high treatment success rate, but there is still a risk of recurrence. Cisplatin, which is one of the chemotherapeutic agents used for its treatment, is associated with a very high rate of resistance. Par-4 (prostate apoptosis response 4) is a tumor suppressor, which is capable of sensitizing tumor cells to chemotherapy. Therefore, the aim of this study was to determine whether combined treatment with Par-4 and cisplatin is effective for inhibiting growth of Wilms' tumor. Wilms' tumor and control cell samples were collected and analyzed by immunofluorescence assay and immunohistochemistry...
July 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28719889/combined-23-g-pars-plana-vitrectomy-and-lensectomy-in-the-management-of-glaucoma-associated-with-nanophthalmos
#19
Zhaotian Zhang, Shaochong Zhang, Xintong Jiang, Yantao Wei
PURPOSE: To investigate the surgical results of 23-G pars plana vitrectomy (PPV) combined with lensectomy (PPL) in nanophthalmic patients with angle closure glaucoma. METHODS: This prospective nonrandomized study included consecutive patients with nanophthalmic glaucoma recruited for 23-G PPV combined with PPL. Best-corrected visual acuity (BCVA), intraocular pressure (IOP), the number of antiglaucoma medications, and surgery-associated complications were recorded...
July 19, 2017: Ophthalmic Research
https://www.readbyqxmd.com/read/28719739/graphene-electronic-tattoo-sensors
#20
Shideh Kabiri Ameri, Rebecca Ho, Hongwoo Jang, Li Tao, Youhua Wang, Liu Wang, David M Schnyer, Deji Akinwande, Nanshu Lu
Tattoo-like epidermal sensors are an emerging class of truly wearable electronics owing to their thinness and softness. While most of them are based on thin metal films, silicon membrane, or nanoparticle-based printable inks, we report a sub-micron thick, multimodal electronic tattoo sensors that are made of graphene. The graphene electronic tattoo (GET) is designed with filamentary serpentines and fabricated by a cost- and time-effective "wet transfer, dry patterning" method. It has a total thickness of 463 ± 30 nm, an optical transparency of ~85%, and a stretchability of more than 40%...
July 18, 2017: ACS Nano
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