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https://www.readbyqxmd.com/read/29155722/automated-gait-analysis-in-mice-with-chronic-constriction-injury
#1
Dong-Wook Kang, Jae-Gyun Choi, Ji-Young Moon, Suk-Yun Kang, Yeonhee Ryu, Jin Bong Park, Hyun-Woo Kim
The von Frey test is a classical method that has been widely used to examine the sensory function of neuropathic pain animals. However, it has some disadvantages such as subjective data and the requirement of a skilled, experienced experimenter. To date, a variety of modifications have improved the von Frey method, but it still has a few limitations. Recent reports have suggested that gait analysis produces more accurate and objective data from the neuropathic animals. This protocol demonstrates how to perform the automated gait analysis to determine the degree of neuropathic pain in mice...
October 17, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29154910/endoscopic-string-clip-suturing-method-a-prospective-pilot-study-with-video
#2
Toshihiro Nishizawa, Teppei Akimoto, Toshio Uraoka, Yutaka Mitsunaga, Tadateru Maehata, Yasutoshi Ochiai, Ai Fujimoto, Osamu Goto, Takanori Kanai, Naohisa Yahagi
BACKGROUND AND AIM: We developed a suturing method with string and clips for a single-channel endoscope. The feasibility of string clip suturing method was evaluated in this prospective pilot study. METHODS: This study involved consecutive 10 patients who underwent endoscopic submucosal dissection (ESD) for duodenal tumor. Polyester string is tied to the arm part of partially outthrusted clip. Clip and string can be passed through an instrument channel (3.2 mm) of a single-channel endoscope...
November 15, 2017: Gastrointestinal Endoscopy
https://www.readbyqxmd.com/read/29154794/the-relative-persuasiveness-of-narrative-versus-non-narrative-health-messages-in-public-health-emergency-communication-evidence-from-a-field-experiment
#3
Mesfin A Bekalu, Cabral A Bigman, Rachel F McCloud, Leesa K Lin, K Viswanath
Previous studies indicated that narrative health messages are more effective than non-narrative messages in influencing health outcomes. However, this body of evidence does not account for differences in health domain, and little is known about the effectiveness of this message execution strategy during public health emergencies. In this study, we examined the relative effectiveness of the two formats in influencing knowledge and perceived response efficacy related to prevention of pandemic influenza, and determined whether effects of message format vary across population sub-groups...
November 14, 2017: Preventive Medicine
https://www.readbyqxmd.com/read/29154585/cognitive-reappraisal-and-acceptance-effects-on-emotion-physiology-and-perceived-cognitive-costs
#4
Allison S Troy, Amanda J Shallcross, Anna Brunner, Rachel Friedman, Markera C Jones
Two emotion regulation strategies-cognitive reappraisal and acceptance-are both associated with beneficial psychological health outcomes over time. However, it remains unclear whether these 2 strategies are associated with differential consequences for emotion, physiology, or perceived cognitive costs in the short-term. The present study used a within-subjects design to examine the effects of reappraisal (reframing one's thoughts) and acceptance (accepting feelings without trying to control or judge them) on the subjective experience of negative emotions, positive emotions, and physiological responses during and following recovery from sad film clips shown in the laboratory...
November 20, 2017: Emotion
https://www.readbyqxmd.com/read/29152467/an-unruptured-anterior-communicating-artery-aneurysm-presenting-with-left-homonymous-hemianopsia-a-case-report
#5
Jae Wook Kim, Taek Kyun Nam, Ki Su Park, Yong Sook Park, Jeong Taik Kwon
Unruptured cerebral aneurysms sometimes present with visual symptomsdue to compression of the visual pathways. However, until now, unruptured anterior communicating artery (ACoA) aneurysms presenting visual field defects have been extremely rare. The authors report the case of a 51-year-old woman who presented with left homonymous hemianopsia. Radiological findings demonstrated an ACoA aneurysm filled with thrombus, that was compressing the optic chiasm and post-chiasmal tract. The patient underwent clipping of the aneurysm, which resolved the visual field defect...
June 2017: Journal of Cerebrovascular and Endovascular Neurosurgery
https://www.readbyqxmd.com/read/29152466/a-less-invasive-strategy-for-ruptured-cerebral-aneurysms-with-intracerebral-hematomas-endovascular-coil-embolization-followed-by-stereotactic-aspiration-of-hematomas-using-urokinase
#6
Sang Heum Kim, Tae Gon Kim, Min Ho Kong
Objective: Aneurysm clipping and simultaneous hematoma evacuation through open craniotomy is traditionally recommended for ruptured cerebral aneurysms accompanied by intracerebral or intrasylvian hemorrhages. We report our experience of adapting a less invasive treatment strategy in poor-grade patients with intracerebral or intrasylvian hemorrhages associated with ruptured cerebral aneurysms, where the associated ruptured cerebral aneurysms were managed by endovascular coil embolization, followed by stereotactic aspiration of hematomas (SRH) using urokinase...
June 2017: Journal of Cerebrovascular and Endovascular Neurosurgery
https://www.readbyqxmd.com/read/29150605/global-role-of-the-bacterial-post-transcriptional-regulator-csra-revealed-by-integrated-transcriptomics
#7
Anastasia H Potts, Christopher A Vakulskas, Archana Pannuri, Helen Yakhnin, Paul Babitzke, Tony Romeo
CsrA is a post-transcriptional regulatory protein that is widely distributed among bacteria. This protein influences bacterial lifestyle decisions by binding to the 5' untranslated and/or early coding regions of mRNA targets, causing changes in translation initiation, RNA stability, and/or transcription elongation. Here, we assess the contribution of CsrA to gene expression in Escherichia coli on a global scale. UV crosslinking immunoprecipitation and sequencing (CLIP-seq) identify RNAs that interact directly with CsrA in vivo, while ribosome profiling and RNA-seq uncover the impact of CsrA on translation, RNA abundance, and RNA stability...
November 17, 2017: Nature Communications
https://www.readbyqxmd.com/read/29148610/can-coordination-driven-supramolecular-self-assembly-reactions-be-conducted-from-fully-aliphatic-linkers
#8
Christophe Lescop, Mehdi Elsayed Moussa, Sloane Evariste, Barbara Kräemer, Régis Réau, Manfred Scheer
A series of fully aliphatic cyano-capped ditopic linkers with increasing lengths were reacted with a pre-assembled Cu(I) bimetallic molecular clip bearing short intermetallic distances. It is shown that, depending on the length of the ditopic linkers, a rational design of unprecedented supramolecular compact metallacycles bearing fully aliphatic walls is possible. The specific pre-organized molecular arrangement of the molecular clip used favors stabilizing interlinker London dispersion interactions allowing, as the linkers' lengths increase, the selective formation of discrete compact metallacycles at the expense of 1D coordination polymers...
November 17, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/29147735/reduction-of-metallic-artifacts-of-the-post-treatment-intracranial-aneurysms-effects-of-single-energy-metal-artifact-reduction-algorithm
#9
Yu-Ning Pan, Ge Chen, Ai-Jing Li, Zhao-Qian Chen, Xiang Gao, Yi Huang, Bradley Mattson, Shan Li
PURPOSE: This study evaluated the quality of computed tomography (CT) and CT angiography images generated using the single-energy metal artifact reduction (SEMAR) algorithm during perfusion examination in patients who had undergone reconstruction with neurosurgical clipping or endovascular coiling for treatment of aneurysms. METHODS: A total of 55 patients with implanted intracranial clips or coils (24 men and 31 women; mean age 60.15 ± 15.86 years) underwent perfusion studies evaluated by CT and CT angiography with a 320-row CT scanner...
November 16, 2017: Clinical Neuroradiology
https://www.readbyqxmd.com/read/29146908/lrpprc-mediated-folding-of-the-mitochondrial-transcriptome
#10
Stefan J Siira, Henrik Spåhr, Anne-Marie J Shearwood, Benedetta Ruzzenente, Nils-Göran Larsson, Oliver Rackham, Aleksandra Filipovska
The expression of the compact mammalian mitochondrial genome requires transcription, RNA processing, translation and RNA decay, much like the more complex chromosomal systems, and here we use it as a model system to understand the fundamental aspects of gene expression. Here we combine RNase footprinting with PAR-CLIP at unprecedented depth to reveal the importance of RNA-protein interactions in dictating RNA folding within the mitochondrial transcriptome. We show that LRPPRC, in complex with its protein partner SLIRP, binds throughout the mitochondrial transcriptome, with a preference for mRNAs, and its loss affects the entire secondary structure and stability of the transcriptome...
November 16, 2017: Nature Communications
https://www.readbyqxmd.com/read/29146726/endovascular-rescue-treatment-through-stent-positioning-after-surgical-clipping-of-intracranial-aneurysms-complicated-by-parent-artery-obstruction
#11
Giovanni Vitale, Giacomo Talenti, Joseph Gabrieli, Giacomo Cester, Alessandro Della Puppa, Francesco Causin
During aneurysm clipping, ischemic complications may occur and require a rescue treatment, usually surgical. We present two such cases that required urgent rescue treatment, performed through an endovascular route. Our first case presented with a non-ruptured right posterior inferior cerebellar artery (PICA) aneurysm. After permanent clip positioning, occlusion of the PICA occurred. An unsuccessful attempt of clip repositioning was performed. We therefore positioned a stent obtaining complete flow restoration...
November 16, 2017: BMJ Case Reports
https://www.readbyqxmd.com/read/29144538/control-of-hemorrhage-through-the-osteotomy-gap-during-tibial-plateau-leveling-osteotomy-9-cases
#12
Luis Matres-Lorenzo, Aidan McAlinden, Antoine Bernardé, Fabrice Bernard
OBJECTIVE: To describe a new technique to control intraoperative hemorrhage during tibial plateau leveling osteotomy (TPLO) and report subsequent short-term and long-term complications. STUDY DESIGN: Retrospective case series. ANIMALS: Nine dogs with arterial hemorrhage during TPLO. METHODS: Medical records of dogs with hemorrhage controlled through the osteotomy gap of a TPLO were reviewed at 2 institutions over 8 years...
November 16, 2017: Veterinary Surgery: VS
https://www.readbyqxmd.com/read/29143089/surgical-treatment-of-gastro-pulmonary-fistula-following-bariatric-surgery-possible-and-safe
#13
Alon Ben Nun, David Simansky, Merav Rokah, Nona Zeitlin, Nir Golan, Ramez Abu Khalil, Michalle Soudack
BACKGROUND: Laparoscopic sleeve gastrectomy is a common surgical management of morbid obesity. Major complication rate is 3-8%. Staple line leak is one of the most serious complications. In a small group of patients, a gastro-pulmonary fistula is formed. Endoscopic and minimally invasive measures are the first line of treatment with considerable success rate. There are very poor data in the literature what should be done in cases of failure. In this paper, we report our positive experience with definitive surgical repair...
November 15, 2017: World Journal of Surgery
https://www.readbyqxmd.com/read/29142855/single-port-thoracoscopic-treatment-of-thoracic-duct-injury-after-thyroidectomy-with-neck-dissection
#14
Serkan Teksoz, Ezel Ersen, Akif Enes Arikan, Sina Ferahman, Kamil Kaynak, Gianlorenzo Dionigi, Yusuf Bukey
Chylous leakage is a complication of thyroidectomy accompanied by bilateral neck dissection with incidence of 0.5-6.2%. A 51-year-old female patient underwent total thyroidectomy, bilateral and central neck dissection for papillary thyroid carcinoma. In post-operative 4(th) day, left sided chylous leakage was observed as 1,500 cc/day through neck drain. Leakage did not cease after 1-month conservative treatment so single port thoracoscopic intervention was performed. Under general anesthesia, patient was placed in left lateral decubitus position...
October 2017: Gland Surgery
https://www.readbyqxmd.com/read/29142186/endoscopic-treatment-of-boerhaave-syndrome-using-polyglycolic-acid-sheets-and-fibrin-glue-a-report-of-two-cases
#15
Yumiko Ishikawa, Takashi Tagami, Hayato Hirashima, Reo Fukuda, Yuuta Moroe, Kyoko Unemoto
Boerhaave syndrome, the spontaneous perforation of the esophagus, is an emergency, life-threatening condition. Current endoscopic treatment options include clipping and stenting, but the use of polyglycolic acid (PGA) sheets for treating the condition has not been reported. In recent years, PGA sheets have been used after endoscopic submucosal dissection to prevent perforations and stricture formation in patients treated for early-stage carcinoma. We report the cases of two patients with Boerhaave syndrome who were successfully treated using PGA sheets...
2017: Journal of Nippon Medical School, Nippon Ika Daigaku Zasshi
https://www.readbyqxmd.com/read/29140849/endoscopic-submucosal-dissection-for-superficial-proximal-esophageal-neoplasia-is-highly-successful
#16
Yi-Qun Zhang, Tao Chen, Chen Zhang, Quan-Lin Li, Wei-Feng Chen, Li-Qing Yao, Ping-Hong Zhou, Mei-Dong Xu
OBJECTIVE: The aim of this study was to evaluate the outcomes of endoscopic submucosal dissection (ESD) for superficial proximal esophageal neoplasia. SUMMARY OF BACKGROUND DATA: The surgery for a tumor located in proximal esophagus is relatively difficult and leads to a high morbidity and mortality. ESD is a minimally invasive endoscopic treatment of superficial neoplasia of the gastrointestinal tract allowing en block resection with low recurrence rates; however, ESD for superficial proximal esophageal neoplasia is little known...
December 2017: Annals of Surgery
https://www.readbyqxmd.com/read/29140644/evaluation-of-low-cost-hydrogen-sulfide-monitors-for-use-in-livestock-production
#17
Jessica M Beswick-Honn, Thomas M Peters, T Renée Anthony
Direct-reading gas monitors warn workers of the risk of potentially fatal hydrogen sulfide (H2S) exposures that may arise during manure handling. Low-cost, low-maintenance H2S monitors are available from many manufacturers, but differences in their features and performance make selection challenging for farmers. Moreover, little information is available on the practical maintenance and performance of these devices in agricultural environments. The objective of this study was to provide information to agricultural workers to aid in the selection, maintenance, and use of low-cost H2S monitors...
November 20, 2017: Journal of Agricultural Safety and Health
https://www.readbyqxmd.com/read/29140523/sequential-coiling-assisted-deployment-of-flow-diverter-for-treatment-of-fusiform-middle-cerebral-artery-aneurysms
#18
Abdulrahman Y Alturki, Philip G R Schmalz, Christopher S Ogilvy, Ajith J Thomas
BACKGROUND AND IMPORTANCE: Fusiform intracranial aneurysms remain challenging lesions to treat. These aneurysms have historically required bypass procedures or clip remodeling constructs for cure. Recently, endovascular specialists have reported experience with flow diversion for complex fusiform aneurysms of the vertebrobasilar system, with mixed results. Vascular anatomy for anterior circulation fusiform aneurysms may make these lesions more amenable to flow diversion and embolization procedures; however, published experience with these techniques is lacking...
November 11, 2017: Operative Neurosurgery (Hagerstown, Md.)
https://www.readbyqxmd.com/read/29138975/distal-transsylvian-keyhole-approach-for-unruptured-anterior-circulation-small-aneurysms
#19
Ririko Takeda, Hiroki Kurita
BACKGROUND: To reduce complications associated with conventional pterional craniotomy, a transsylvian keyhole approach for unruptured small anterior circulation aneurysms is proposed. METHODS: A 7-cm linear scalp incision is made along the hairline, beginning at the zygoma, followed by minimal temporal muscle dissection. Two burr holes are drilled out at McCarty's point and the temporal bone, and a 3-cm equilateral triangle bone flap is made, whose apex is located above the sylvian point...
November 14, 2017: Acta Neurochirurgica
https://www.readbyqxmd.com/read/29137810/technique-for-precaval-and-laterocaval-nodes-excision-at-extraperitoneal-paraaortic-lymphadenectomy
#20
M Gorostidi, C Villalain, R Ruiz, I Jaunarena, A Lekuona
STUDY OBJECTIVE: To describe our technique for excision of the pre-caval and laterocaval nodes using an extraperitoneal approach. This technique was developed to make the dissection and excision of the less accessible nodes in an easier and safer way by minimizing the risk of great vessels injury and bleeding. DESIGN: Step-by-step description of the surgical procedure using video (Canadian Task Force classification III). SETTING: The procedure was performed at a teaching hospital, Hospital Universitario Donostia (Spain)...
November 11, 2017: Gynecologic Oncology
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