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https://www.readbyqxmd.com/read/29651234/pathophysiology-of-long-non-coding-rnas-in-ischemic-stroke
#1
REVIEW
Weimin Ren, Xiaobo Yang
Stroke is a neurological disease with high disability and fatality rates, and ischemic stroke accounts for 75% of all stroke cases. The underlying pathophysiologic processes of ischemic stroke include oxidative stress, toxicity of excitatory amino acids, excess calcium ions, increased apoptosis and inflammation. Long non-coding RNAs (lncRNAs) may participate in the regulation of the pathophysiologic processes of ischemic stroke as indicated by altered expression of lncRNAs in blood samples of acute ischemic stroke patients, animal models of focal cerebral ischemia and oxygen-glucose deprivation (OGD) cell models...
2018: Frontiers in Molecular Neuroscience
https://www.readbyqxmd.com/read/29629712/l-theanine-protects-h9c2-cells-from-hydrogen-peroxide-induced-apoptosis-by-enhancing-antioxidant-capability
#2
Chengjian Li, Qiongxian Yan, Shaoxun Tang, Wenjun Xiao, Zhiliang Tan
BACKGROUND L-theanine is a non-protein amino acid in green tea, and its hepatoprotection and neuroprotection have been verified. However, whether L-theanine can prevent cardiomyocytes from apoptosis is unclear yet. This study evaluated the protective effects of L-theanine on H2O2-induced heart injury in vitro. MATERIAL AND METHODS The certified H9C2 cells were pretreated with L-theanine (0 mM, 4 mM, 8 mM, and 16 mM) for 24 h, followed by 160 µM H2O2 solution for 4 h. The cell viability and antioxidant indices were assayed...
April 9, 2018: Medical Science Monitor: International Medical Journal of Experimental and Clinical Research
https://www.readbyqxmd.com/read/29625260/protective-effects-of-polygonum-multiflorum-on-ischemic-stroke-rat-model-analysed-by-1-h-nmr-metabolic-profiling
#3
Ming-Hui Li, Ling-Yu Ruan, Cheng Chen, Yue-Xiao Xing, Wei Hong, Rong-Hui Du, Jun-Song Wang
Stroke is the third most common cause of death in most industrialized countries. Polygonum multiflorum (He-Shou-Wu, HSW) is one of the traditional Chinese medicines with multiple pharmacological activities which is widely used in Chinese recipe. This study aims to explore the protective effect of HSW on ischemic stroke rat model and to elucidate the underlying mechanisms. The mortality rate, neurological deficit, cerebral infarct size, histopathology, immunohistochemistry, biochemical parameters, quantitative real-time polymerase chain reaction and western blotting were used to access the treatment effects of HSW on ischemic stroke...
March 31, 2018: Journal of Pharmaceutical and Biomedical Analysis
https://www.readbyqxmd.com/read/29596325/preconditioning-like-properties-of-short-term-hypothermia-in-isolated-perfused-rat-liver-iprl-system
#4
Norma Alva, Raquel G Bardallo, David Basanta, Jesús Palomeque, Teresa Carbonell
Hypothermia may attenuate the progression of ischemia-induced damage in liver. Here, we determined the effects of a brief cycle of hypothermic preconditioning applied before an ischemic/reperfusion (I/R) episode in isolated perfused rat liver (IPRL) on tissue damage and oxidative stress. Rats (male, 200-250 g) were anaesthetised with sodium pentobarbital (60 mg·kg-1 i.p) and underwent laparatomy. The liver was removed and perfused in a temperature-regulated non-recirculating system. Livers were randomly divided into two groups ( n = 6 each group)...
March 29, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29570992/amino-acid-restriction-triggers-angiogenesis-via-gcn2-atf4-regulation-of-vegf-and-h-2-s-production
#5
Alban Longchamp, Teodelinda Mirabella, Alessandro Arduini, Michael R MacArthur, Abhirup Das, J Humberto Treviño-Villarreal, Christopher Hine, Issam Ben-Sahra, Nelson H Knudsen, Lear E Brace, Justin Reynolds, Pedro Mejia, Ming Tao, Gaurav Sharma, Rui Wang, Jean-Marc Corpataux, Jacques-Antoine Haefliger, Kyo Han Ahn, Chih-Hao Lee, Brendan D Manning, David A Sinclair, Christopher S Chen, C Keith Ozaki, James R Mitchell
Angiogenesis, the formation of new blood vessels by endothelial cells (ECs), is an adaptive response to oxygen/nutrient deprivation orchestrated by vascular endothelial growth factor (VEGF) upon ischemia or exercise. Hypoxia is the best-understood trigger of VEGF expression via the transcription factor HIF1α. Nutrient deprivation is inseparable from hypoxia during ischemia, yet its role in angiogenesis is poorly characterized. Here, we identified sulfur amino acid restriction as a proangiogenic trigger, promoting increased VEGF expression, migration and sprouting in ECs in vitro, and increased capillary density in mouse skeletal muscle in vivo via the GCN2/ATF4 amino acid starvation response pathway independent of hypoxia or HIF1α...
March 22, 2018: Cell
https://www.readbyqxmd.com/read/29560630/-effect-of-electroacupuncture-at-neiguan-pc-6-on-serum-and-myocardial-metabolites-in-rats-with-myocardial-ischemia-reperfusion-injury-based-on-nuclear-magnetic-resonance-spectroscopy
#6
Ya-Ni Tang, Cheng-Fu Tan, Wei-Wei Liu, Jie Yan, Chao Wang, Mi Liu, Dong-Hai Lin, Cai-Hua Huang, Lin Du, Mei-Lin Chen, Jiao-Lan Li, Ding-Ming Zhu
OBJECTIVE: We have repeatedly demonstrated that electroacupuncture (EA) of "Neiguan"(PC 6) can improve myocardial ischemia in rats. The present study was designed to investigate the metabolomic profile of peripheral blood se-rum and myocardium involving EA-induced improvement of myocardial ischemia-reperfusion injury (MIRI) in rats by using nuclear magnetic resonance spectroscopy. METHODS: Thirty male SD rats were equally randomized into blank control, model and EA groups...
March 25, 2018: Zhen Ci Yan Jiu, Acupuncture Research
https://www.readbyqxmd.com/read/29547062/tissue-type-plasminogen-activator-protects-the-postsynaptic-density-in-the-ischemic-brain
#7
Valerie Jeanneret, Juan P Ospina, Ariel Diaz, Luis G Manrique, Paola Merino, Laura Gutierrez, Enrique Torre, Fang Wu, Lihong Cheng, Manuel Yepes
Cerebral ischemia causes the presynaptic release of tissue-type plasminogen activator (tPA). The postsynaptic density (PSD) is a postsynaptic structure that provides a matrix where signaling transduction of excitatory synapses takes place. The postsynaptic density protein-95 (PSD-95) is the most abundant scaffolding protein in the postsynaptic density (PSD), where it modulates the postsynaptic response to the presynaptic release of glutamate by regulating the anchoring of glutamate receptors to the PSD. We found that tPA induces the local translation of PSD-95 mRNA and the subsequent recruitment of PSD-95 protein to the PSD, via plasminogen-independent activation of TrkB receptors...
January 1, 2018: Journal of Cerebral Blood Flow and Metabolism
https://www.readbyqxmd.com/read/29493139/-protective-effects-of-ginkgo-terpene-lactones-meglumine-injection-on-focal-cerebral-ischemia-in-rats
#8
Yan-Ping Luo, Hong Zhang, Han-Fei Hu, Ze-Yu Cao, Xin-Zhuang Zhang, Liang Cao, Zhen-Zhong Wang, Wei Xiao
To investigate the protective effects of ginkgo diterpene lactone meglumine injection (GDLMI) on cerebral focal ischemia reperfusion injury induced by middle cerebral artery occlusion (MCAO) in rats, and explore its possible mechanism. One hundred and forty male SD rats were randomly divided into sham operation group, model group, ginkgo biloba extract injection (Ginaton, 1.0 mL•kg⁻¹) group, nimodipine (0.4 mg•kg⁻¹) group, and GDLMI (5.2, 2.6, 1.3 mg•kg⁻¹) groups; All of rats received corresponding drugs by tail vein injection 4 days before operation (normal saline in model group and sham operation group)...
December 2017: Zhongguo Zhong Yao za Zhi, Zhongguo Zhongyao Zazhi, China Journal of Chinese Materia Medica
https://www.readbyqxmd.com/read/29489588/disruption-of-the-glua2-gapdh-complex-using-tat-glua2nt1-3-2-peptide-protects-against-ampar-mediated-excitotoxicity-after-epilepsy
#9
Jinghui Zhang, Nana Qiao, Xiufang Ding, Jiwen Wang
Excitotoxicity and neuronal death following epilepsy involve α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid receptors (AMPARs). It forms a protein complex with glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and co-internalizes upon activation of AMPA receptors after epilepsy. Disruption of the GluA2/GAPDH complex with an interfering peptide, TAT-GluA2NT1-3-2, protects cells against AMPAR-mediated excitotoxicity, which have been identified in in-vitro and in-vivo models of brain ischemia. We postulated that disruption of the GluA2/GAPDH interaction with the TAT-GluA2NT1-3-2 peptide would also protect against AMPAR-induced neuronal injury in an in-vivo model of status epilepticus (SE)...
March 21, 2018: Neuroreport
https://www.readbyqxmd.com/read/29477465/-1-h-nmr-metabolic-signature-of-cerebrospinal-fluid-following-repetitive-lower-limb-remote-ischemia-preconditioning
#10
Hailiang Wang, Zhiyong He, Yijue Zhang, Jun Zhang
Background OBJECTIVE: The cerebral ischemia/reperfusion greatly influences brain metabolism. Remote ischemia preconditioning (RIPC) is reported to confer neuroprotective effects against cerebral ischemia in animal models and human. This study aims to investigate the metabolomic profiles of cerebrospinal fluid (CSF) in patients treated with repetitive lower limb RIPC and provides an insight into possible mechanism underlying RIPC-induced neuroprotection. METHOD: Fifty healthy patients undergoing minor surgery under spinal anesthesia were randomly allocated to 2 groups: control group (Group C, n = 25) and RIPC treatment group (Group T,n = 25)...
February 22, 2018: Neurochemistry International
https://www.readbyqxmd.com/read/29358855/human-small-intestine-is-capable-of-restoring-barrier-function-after-short-ischemic-periods
#11
Dirk Hsm Schellekens, Inca Hr Hundscheid, Claire Aji Leenarts, Joep Grootjans, Kaatje Lenaerts, Wim A Buurman, Cornelis Hc Dejong, Joep Pm Derikx
AIM: To assess intestinal barrier function during human intestinal ischemia and reperfusion (IR). METHODS: In a human experimental model, 6 cm of jejunum was selectively exposed to 30 min of ischemia (I) followed by 30 and 120 min of reperfusion (R). A sham procedure was also performed. Blood and tissue was sampled at all-time points. Functional barrier function was assessed using dual-sugar absorption tests with lactulose (L) and rhamnose (R). Plasma concentrations of citrulline, an amino acid described as marker for enterocyte function were measured as marker of metabolic enterocytes restoration...
December 28, 2017: World Journal of Gastroenterology: WJG
https://www.readbyqxmd.com/read/29350679/proangiogenic-functions-of-an-rgd-slay-containing-osteopontin-icosamer-peptide-in-huvecs-and-in-the-postischemic-brain
#12
Hahnbie Lee, Yin-Chuan Jin, Seung-Woo Kim, Il-Doo Kim, Hye-Kyung Lee, Ja-Kyeong Lee
Osteopontin (OPN) is a phosphorylated glycoprotein secreted into body fluids by various cell types. OPN contains arginine-glycine-aspartate (RGD) and serine-leucine-alanine-tyrosine (SLAY) motifs that bind to several integrins and mediate a wide range of cellular processes. In the present study, the proangiogenic effects of a 20-amino-acid OPN peptide (OPNpt20) containing RGD and SLAY motifs were examined in human umbilical vein endothelial cells (HUVECs) and in a rat focal cerebral ischemia model. OPNpt20 exerted robust proangiogenic effects in HUVECs by promoting proliferation, migration and tube formation...
January 19, 2018: Experimental & Molecular Medicine
https://www.readbyqxmd.com/read/29349722/bystin-bysl-as-a-possible-marker-of-severe-hypoxic-ischemic-changes-in-neuropathological-examination-of-forensic-cases
#13
Mieszko Olczak, Dominik Chutorański, Magdalena Kwiatkowska, Dorota Samojłowicz, Sylwia Tarka, Teresa Wierzba-Bobrowicz
Bystin (BYSL) is a 306-amino acid protein encoded in humans by the BYSL gene located on the 6p21.1 chromosome. It is conserved across a wide range of eukaryotes. BYSL was reported to be a sensitive marker for the reactive astrocytes induced by ischemia/reperfusion and chemical hypoxia in vitro and is considered to be one of the common characteristics of astrogliosis. In our study we examined whether BYSL could be used as a marker for hypoxic-ischemic changes in forensic cases. Groups suspected of acute hypoxic-ischemic changes presented strong BYSL expression in the cytoplasm of neocortical neurons especially in layers 3-5, that seemed to be short-lasting...
March 2018: Forensic Science, Medicine, and Pathology
https://www.readbyqxmd.com/read/29187590/selective-cannabinoid-2-receptor-stimulation-reduces-tubular-epithelial-cell-damage-after-renal-ischemia-reperfusion-injury
#14
Jeffrey D Pressly, Suni M Mustafa, Ammaar H Adibi, Sahar Alghamdi, Pankaj Pandey, Kuldeep K Roy, Robert J Doerksen, Bob M Moore, Frank Park
Ischemia-reperfusion injury (IRI) is a common cause of acute kidney injury (AKI), which is an increasing problem in the clinic and has been associated with elevated rates of mortality. Therapies to treat AKI are currently not available, so identification of new targets that can be modulated to ameliorate renal damage upon diagnosis of AKI is essential. In this study, a novel cannabinoid receptor 2 (CB2) agonist, SMM-295 [3'-methyl-4-(2-(thiophen-2-yl)propan-2-yl)biphenyl-2,6-diol], was designed, synthesized, and tested in vitro and in silico...
February 2018: Journal of Pharmacology and Experimental Therapeutics
https://www.readbyqxmd.com/read/29174035/effects-of-standard-ethanolic-extract-from-erythrina-velutina-in-acute-cerebral-ischemia-in-mice
#15
Francisca Taciana Sousa Rodrigues, Caren Nádia Soares de Sousa, Naiara Coelho Ximenes, Anália Barbosa Almeida, Lucas Moraes Cabral, Cláudio Felipe Vasconcelos Patrocínio, Aline Holanda Silva, Luzia Kalyne Almeida Moreira Leal, José Eduardo Ribeiro Honório Júnior, Danielle Macedo, Silvânia Maria Mendes Vasconcelos
The objective of this study was to verify a possible neuroprotective effect of the ethanolic extract of Erythrina velutina (EEEV). Male Swiss mice were submitted to transient cerebral ischemia by occlusion of both carotid arteries for 30 min and treated for 5 days with EEEV (200 or 400 mg/kg) or Memantine (MEM) 10 mg/kg, with initiation of treatment 2 or 24 h after Ischemia. On the 6th day after the induction of ischemia, the animals were submitted to evaluation of locomotor activity and memory and then sacrificed...
December 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29115616/metabolic-alterations-in-the-rat-cerebellum-following-acute-middle-cerebral-artery-occlusion-as-determined-by-1h-nmr-spectroscopy
#16
Zi-Long Hu, Huan-Huan Xia, Yun-Jun Yang, Hong Zheng, Liang-Cai Zhao, Yong-Chun Chen, Qi-Chuan Zhuge, Neng-Zhi Xia, Hong-Chang Gao, Wei-Jian Chen
Supratentorial focal ischemia may reduce cerebral blood volume and cerebellar glucose metabolic rate contralateral to the region of ischemia. The present study investigated the effects of middle cerebral artery occlusion (MCAO) on cerebral metabolism in the ischemic cerebral hemisphere and the non‑ischemic cerebellum in rats 1, 3, 9 and 24 h following ischemia using ex vivo proton nuclear magnetic resonance (1H NMR) spectroscopy. The results demonstrated that focal ischemia induced increases in the levels of lactate and alanine, and a decrease in succinate, as early as 1 h following ischemia in the left cerebral hemisphere and the right cerebellum...
January 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29089759/in-vitro-and-in-vivo-protein-release-and-anti-ischemia-reperfusion-injury-properties-of-bone-morphogenetic-protein-2-loaded-glycyrrhetinic-acid-poly-ethylene-glycol-b-poly-l-lysine-nanoparticles
#17
Fang Shan, YuJuan Liu, Haiying Jiang, Fei Tong
Here, we describe a bone morphogenetic protein-2 (BMP-2) nanocarrier based on glycyrrhetinic acid (GA)-poly(ethylene glycol) (PEG)-b-poly(l-lysine) (PLL). A protein nanocarrier was synthesized, characterized and evaluated as a BMP-2 delivery system. The designed nanocarrier was synthesized based on the ring-opening polymerization of amino acid N-carboxyanhydride. The final product was measured with1 H nuclear magnetic resonance. GA-PEG-b-PLL nanocarrier could combine with BMP-2 through electrostatic interaction to form polyion complex (PIC) micelles...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/29043002/humanin-analogue-s14g-humanin-has-neuroprotective-effects-against-oxygen-glucose-deprivation-reoxygenation-by-reactivating-jak2-stat3-signaling-through-the-pi3k-akt-pathway
#18
Guang-Sheng Gao, Yun Li, Heng Zhai, Jing-Wen Bi, Fu-Sen Zhang, Xiao-Ying Zhang, Shao-Hua Fan
Stroke, characterized by a disruption of blood supply to the brain, is a major cause of morbidity and mortality worldwide. Although humanin, a 24-amino acid polypeptide, has been identified to have multiple neuroprotective functions, the level of humanin in plasma has been demonstrated to decrease with age, which likely limits the effects against stroke injury. A potent humanin analogue, S14G-humanin (HNG), generated by replacement of Ser14 with glycine, has been demonstrated to have 1,000-fold stronger biological activity than humanin...
October 2017: Experimental and Therapeutic Medicine
https://www.readbyqxmd.com/read/29035834/synergistic-effect-of-mild-hypothermia-and-the-notch-inhibitor-dapt-against-post-stroke-seizures
#19
Guo-Shuai Yang, Xiao-Yan Zhou, Xue-Fang An, Xuan-Jun Liu, Yan-Jun Zhang, Dan Yu
Seizure is a serious complication of stroke, indicating poor prognosis. Notch signaling is associated with neuronal activity. Inhibition of Notch signaling suppresses seizure activity induced by kainic acid. The present study investigated the effect of the Notch inhibitor, DAPT, alone or in combination with mild hypothermia, on post-stroke seizures. A global cerebral ischemia (GCI) model was performed in Sprague Dawley (SD) male rats. Seizure activity was evaluated by the frequency of seizure attacks, seizure severity scores, and seizure discharges...
December 2017: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29026150/essential-role-of-the-na-ca2-exchanger-ncx-in-glutamate-enhanced-cell-survival-in-cardiac-cells-exposed-to-hypoxia-reoxygenation
#20
Marta Maiolino, Pasqualina Castaldo, Vincenzo Lariccia, Silvia Piccirillo, Salvatore Amoroso, Simona Magi
Myocardial ischemia culminates in ATP production impairment, ionic derangement and cell death. The provision of metabolic substrates during reperfusion significantly increases heart tolerance to ischemia by improving mitochondrial performance. Under normoxia, glutamate contributes to myocardial energy balance as substrate for anaplerotic reactions, and we demonstrated that the Na+ /Ca2+ exchanger1 (NCX1) provides functional support for both glutamate uptake and use for ATP synthesis. Here we investigated the role of NCX1 in the potential of glutamate to improve energy metabolism and survival of cardiac cells subjected to hypoxia/reoxygenation (H/R)...
October 12, 2017: Scientific Reports
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