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Colon cancer,mir

Junming Chen, Yongmei Xia, Xiaochen Sui, Qingrui Peng, Tongtong Zhang, Jian Li, Jue Zhang
New anticancer agents with lower toxicity have been always urged because of drug resistance associated with overused chemotherapy agents. In this study, steviol, a colonic metabolite of natural sweetener and also a component in leaves of stevia rebaudiana bertoni , was found to possess intensive anticancer activity on the human gastrointestinal cancer cells. Steviol inhibited six human gastrointestinal cancer cells intensively as 5-fluorouracil did at 100 μg/mL. The inhibition mechanism follows mitochondrial apoptotic pathway that was evidenced by increase of Bax/Bcl-2 ratio, activation of p21 and p53; and caspase 3-independent mechanism was also involved...
May 29, 2018: Oncotarget
Junting Ma, Yaping Yang, Yong Fu, Feilong Guo, Xiaoyi Zhang, Shuke Xiao, Weiming Zhu, Zhen Huang, Junfeng Zhang, Jiangning Chen
Rationale: Colitis-associated colorectal cancer (CAC) usually exhibits an accelerated disease progression, an increased resistance to therapeutic drugs and a higher mortality rate than sporadic colorectal cancer (CRC). PIAS3 is a member of the protein inhibitor of activated STAT (PIAS) family; however, little is known about the expression and biological functions of PIAS3 in CAC. The aim of our study was to investigate the biological mechanisms of PIAS3 in CAC. Methods: PIAS3 expression was examined in colon tissues of CAC/CRC patients and azoxymethane-dextran sulfate sodium (AOM-DSS)-induced mice...
2018: Theranostics
Hongliang Cao, Shaojun Huang, Aihua Liu, Zhidan Chen
Objective: The aim of this study is to investigate the expression of miR-155 in colonic cancer tissue and to assess the potential predictive value of miR-155 in colonic cancer patients. Materials and Methods: From March to September of 2011, we included 57 patients with primary colonic cancer who underwent curative surgical resection. Total RNAs were extracted from colonic cancer tissues and adjacent normal tissues. Then the expression of miR-155 in colonic cancer and paracancerous tissues was investigated using real time quantitative reverse transcription-polymerase chain reaction...
April 2018: Journal of Cancer Research and Therapeutics
Chengzhi Huang, Mengya Yu, Xueqing Yao
OBJECTIVE: Although the role of microRNA-17 (miR-17) has been identified as a tumour biomarker in various studies, its prognostic value in cancers remains unclear. Therefore, we performed a systematic review and meta-analysis to analyse and summarise the relationship between the miR-17 status and clinical outcome in a variety of human cancers. DESIGN: Systematic review and meta-analysis. DATA SOURCES: PubMed, Web of Science and Embase from the first year of records to 15 May 2017...
May 31, 2018: BMJ Open
Dehua Zhu, Yefei Sun, Danhua Zhang, Ming Dong, Guiyang Jiang, Xiupeng Zhang, Jianping Zhou
MicroRNA (miR)‑1 is associated with various human malignancies through repressing tumor growth, migration and angiogenesis. Recently, high‑throughput transcriptional profiling confirmed that miR‑1 is markedly downregulated in metastatic colorectal cancer; however, its biological functions and the specific underlying mechanisms in colorectal cancer (CRC) require further investigation. In this study, the expression of miR‑1 in 111 CRC and paired normal tissue samples was measured using quantitative polymerase chain reaction analysis, and the association between miR‑1 expression and clinical characteristics was evaluated...
May 24, 2018: Oncology Reports
Jie Jiang, Hui-Ling Liu, Li Tao, Xian-Yi Lin, Yi-Dong Yang, Si-Wei Tan, Bin Wu
Cystatin SN (cystatin 1, CST1) is a member of the cystatin superfamily which inhibits the proteolytic activity of cysteine proteases. CST1 is a tumor biomarker that provides useful information for the diagnosis of esophageal, gastric and colorectal carcinomas. MicroRNAs (miRNAs or miRs) play an important role in tumor cell proliferation. However, the exact role of let‑7d and CST1 in colon cancer remains unknown. The aim of this study was to assess whether let‑7d inhibits colorectal carcinogenesis through the CST1/p65 pathway, and determine whether it may be used as a potential target for clinical therapy...
May 23, 2018: International Journal of Oncology
Qingkai Meng, Yue Chen, Bo Lian, Yan Shang, Hongmei Yang
MicroRNAs (miRNAs) are suggested to act as either tumor oncogenes or tumor suppressors in different types of cancer. miRNA‑218 (miR‑218) is a type of short, non-coding RNA which is involved in gastric cancer development. In the present study, we evaluated the functions of miR‑218 in SW1417 human colon cancer cells and its potential mechanisms. Following overexpression of miR‑218 in human colon cancer cells, cell viability was determined by CKK‑8 assay, cell apoptosis was observed using a TUNEL Kit, the expression of caspase‑8, and its inhibitor cellular Fas‑associated death domain‑like interleukin‑1β‑converting enzyme inhibitory protein (c‑FLIP) was assessed by RT‑PCR, western blot analysis and immunohistochemistry...
May 23, 2018: Oncology Reports
Xian-Guo Zhou, Xiao-Liang Huang, Si-Yuan Liang, Shao-Mei Tang, Si-Kao Wu, Tong-Tong Huang, Zeng-Nan Mo, Qiu-Yan Wang
Introduction: Colorectal cancer (CRC) is the fourth most common cause of cancer-related mortality worldwide. The tumor, node, metastasis (TNM) stage remains the standard for CRC prognostication. Identification of meaningful microRNA (miRNA) and gene modules or representative biomarkers related to the pathological stage of colon cancer helps to predict prognosis and reveal the mechanisms behind cancer progression. Materials and methods: We applied a systems biology approach by combining differential expression analysis and weighted gene co-expression network analysis (WGCNA) to detect the pathological stage-related miRNA and gene modules and construct a miRNA-gene network...
2018: OncoTargets and Therapy
Min Ho Choe, Yina Yoon, Joon Kim, Sang-Gu Hwang, Young-Hoon Han, Jae-Sung Kim
Although evidence has emerged to suggest that YAP overexpression is a crucial factor for tumor progression and resistance to targeted drugs in multiple cancers, the miRNA-mediated YAP regulation is still unclear. Here we show that the novel miR-550a-3-5p acts as a tumor suppressor and reverses BRAF inhibitor resistance through the direct targeting of YAP. Our data showed that the miR-550a-3-5p suppressed cell proliferation, metastasis, and tumor sphere formation through the direct inhibition of YAP and its oncogenic pathway in various cancer cell types...
May 29, 2018: Cell Death & Disease
Kuijie Liu, Hongliang Yao, Yu Wen, Hua Zhao, Nanjiang Zhou, Sanlin Lei, Li Xiong
Colorectal Cancer (CRC) is one of the most common digestive system malignant tumors. Recently, PDT has been used as a first-line treatment for colon cancer; however, limited curative effect was obtained due to resistance of CRC to PDT. During the past decades, accumulating CRC-related long non-coding RNAs (lncRNAs), microRNAs (miRNAs) and mRNAs have been reported to exert diverse functions through various biological processes; their dysregulation might trigger and/or promote the pathological changes. Herein, we performed microarrays analysis to identify dysregulated lncRNAs, miRNAs and mRNAs in PDT-treated HCT116 cells to figure out the lncRNA-miRNA interactions related to the resistance of CRC to PDT treatment, and the downstream mRNA target, as well as the molecular mechanism...
May 25, 2018: Biochimica et Biophysica Acta
Yongqiang Zhang, Jun Jia, Ying Li, Yan-Ge Chen, Huan Huang, Yang Qiao, Yu Zhu
Glioma is one of the malignant tumor types detrimental to human health; therefore, it is important to find novel targets and therapeutics for this tumor. The downregulated expression of Tudor-staphylococcal nuclease (SN) and alkylglycerone phosphate synthase (AGPS) can decrease cancer malignancy, and the overexpression of them can the increase viability and migration potential of various tumor cell types; however, the role of AGPS in the proliferation and migration of glioma, and the association of Tudor-SN and AGPS in human glioma is not clear...
June 2018: Oncology Letters
Wei-Ping Bi, Min Xia, Xin-Jian Wang
Colorectal cancer is cancer of the colon or rectum and is the third most prevalent form of cancer. Currently, there are several shortcomings in the prognosis and early detection of colon cancer. The present study aims to address questions pertaining to the role of microRNA (miR)-137 in colon cancer progression and the mode of regulation. The endogenous and over-expressed levels of miR-137 in three colon cancer cell lines were assessed by reverse transcription-quantitative polymerase chain reaction (RT-qPCR)...
June 2018: Oncology Letters
Ce Yuan, Michael B Burns, Subbaya Subramanian, Ran Blekhman
Although variation in gut microbiome composition has been linked with colorectal cancer (CRC), the factors that mediate the interactions between CRC tumors and the microbiome are poorly understood. MicroRNAs (miRNAs) are known to regulate CRC progression and are associated with patient survival outcomes. In addition, recent studies suggested that host miRNAs can also regulate bacterial growth and influence the composition of the gut microbiome. Here, we investigated the association between miRNA expression and microbiome composition in human CRC tumor and normal tissues...
May 2018: MSystems
Sandra Parenti, Lucia Montorsi, Sebastian Fantini, Fabiana Mammoli, Claudia Gemelli, Claudio Giacinto Atene, Lorena Losi, Chiara Frassineti, Bruno Calabretta, Enrico Tagliafico, Sergio Ferrari, Tommaso Zanocco-Marani, Alexis Grande
Mesalazine (5-ASA) is an aminosalicylate anti-inflammatory drug capable of inducing μ-protocadherin, a protein expressed by colorectal epithelial cells which is downregulated upon malignant transformation. Treatment with 5-ASA restores μ-protocadherin expression and promotes the sequestration of β-catenin to the plasma membrane. Here we show that 5-ASA-induced μ-protocadherin expression is directly regulated by the KLF4 transcription factor. In addition, we suggest the existence of a dual mechanism whereby 5-ASA-mediated β-catenin inhibition is caused by μ-protocadherin-dependent sequestration of β-catenin to the plasma membrane and by the direct binding of KLF4 to β-catenin...
May 24, 2018: Cancer Prevention Research
Tianzheng Yang, Hongyan Zhai, Ruihong Yan, Zhenhu Zhou, Lei Gao, Luqing Wang
Thyroid cancer is a common malignant tumor. Long non-coding RNA colon cancer-associated transcript 1 (lncRNA CCAT1) is highly expressed in many cancers; however, the molecular mechanism of CCAT1 in thyroid cancer remains unclear. Hence, this study aimed to investigate the effect of CCAT1 on human thyroid cancer cell line FTC-133. FTC-133 cells were transfected with CCAT1 expressing vector, CCAT1 shRNA, miR-143 mimic, and miR-143 inhibitor, respectively. After different treatments, cell viability, proliferation, migration, invasion, and apoptosis were measured...
2018: Brazilian Journal of Medical and Biological Research, Revista Brasileira de Pesquisas Médicas e Biológicas
Wenbin Wang, Xiao Yuan, Aman Xu, Xingyang Zhu, Yanqing Zhan, Shuanhu Wang, Mulin Liu
Deep vein thrombosis (DVT) is a severe clinical process and has a high rate of fatality. Cancer patients have a high incidence rate of venous thrombosis complication and increase the mortality of cancer patients for 2-8 times. The mechanisms involved in human cancers and venous thrombosis remains unclear. In this study, we determined miR-21 expressed higher in human breast cancer, colon cancer and hepatocellular cancer tissues compared with normal tissues and expressed higher in exosomes of breast cancer and hepatocellular cancer cell lines compared with normal cells...
May 16, 2018: Microvascular Research
Joel Pekow, Katherine Meckel, Urszula Dougherty, Haider I Haider, Zifeng Deng, John Hart, David T Rubin, Marc Bissonnette
Identification of biological markers predicting the onset of neoplasia in patients with long-standing ulcerative colitis (UC) could allow for risk stratification in this population. In this study, we retrospectively identified subjects with chronic UC who developed colon neoplasia ( n = 16) matched to UC patients who never developed neoplasia. RNA was extracted from archived colonic biopsies obtained at an interval of 1-2 years prior and 3-5 years prior to the onset of neoplasia. miRNA expression was assessed using Nanostring arrays in 12 subjects, and significantly up-regulated miRNAs were evaluated by real time pcr in the entire cohort of patients...
April 17, 2018: Oncotarget
Pit Ullmann, Fabien Rodriguez, Martine Schmitz, Steffen K Meurer, Komal Qureshi-Baig, Paul Felten, Aurélien Ginolhac, Laurent Antunes, Sonia Frasquilho, Nikolaus Zügel, Ralf Weiskirchen, Serge Haan, Elisabeth Letellier
The vast majority of colorectal cancer (CRC)-related deaths can be attributed to metastatic spreading of the disease. Therefore, deciphering molecular mechanisms of metastatic dissemination is a key prerequisite to improve future treatment options. With this aim, we took advantage of different CRC cell lines and recently established primary cultures enriched in colon cancer stem cells (CSCs) - also known as tumor-initiating cells (TICs) - to identify genes and microRNAs (miRNAs) with regulatory functions in CRC progression...
May 10, 2018: Cancer Research
Haiyi Feng, Ming Xu, Yunpeng Zhang, Bo Han, Jue Wang, Peng Sun
BACKGROUND: The ability to diagnose or treat colorectal cancer (CRC) at an early stage could lead to the improved survival. The purpose of this study was to identify differentially expressed microRNAs (miRNAs) involved in the pathogenesis of CRC. These miRNAs may serve as the biomarkers or potential therapeutic targets for CRC early detection or treatment. METHODS: The GSE39814 miRNA expression profile dataset was downloaded from the NCBI GEO database. MiRNAs were extracted from the exosome fractions of three normal fetal colon-derived cells and HCT116 and SW480 cell lines...
May 1, 2018: Clinical Laboratory
Bijun Wen, Tomas Tokar, Amel Taibi, Jianmin Chen, Igor Jurisica, Elena M Comelli
Citrobacter rodentium is a murine pathogen causing transmissible colonic hyperplasia and colitis with a pathogenic mechanism similar to foodborne enterohaemorrhagic Escherichia coli in humans. Mechanisms underlying intestinal responses to C. rodentium infection are incompletely understood. We identified 24 colonic microRNAs (miRNAs) as significantly deregulated in response to C. rodentium, including miR-7a, -17, -19a, -20a, -20b, -92a, -106a, -132, -200a, and -2137; most of these miRNAs belong to the oncogenic miR-17-92 clusters...
May 1, 2018: Genes and Immunity
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