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Lanlan Huang, Chuangyu Wen, Xiangling Yang, Qiong Lou, Xiaoyan Wang, Jia Che, Junxiong Chen, Zihuan Yang, Xiaojian Wu, Meijin Huang, Ping Lan, Lei Wang, Aikichi Iwamoto, Jianping Wang, Huanliang Liu
PEAK1 is upregulated in multiple human malignancies and has been associated with tumor invasion and metastasis, but little is known about the role of PEAK1 in colorectal cancer (CRC) progression. We investigated the expression pattern, function and regulatory mechanisms of PEAK1 in CRC. Here, we found that PEAK1 is overexpressed in CRC tissues and that high PEAK1 expression predicts poor survival in colon cancer but not rectal cancer. Functionally, silencing PEAK1 inhibits cell proliferation, migration, and invasion in vitro and inhibits the growth of tumor xenografts in nude mice...
February 15, 2018: Cell Death & Disease
Ying Zhang, Kai Li, Dandan Li, Changfeng Li, Bin Zhang
It is well known that microRNA-301a plays an important role in many diseases, as well as is overexpressed in human colon cancer and affects the process of tumorigenesis. Determination of the miR-301a expression provides insight into the mechanism of tumor progression. In this study, we designed a special hairpin deoxyribonucleic acid (DNA) for miR-301a to functionalize gold nanoparticles, which served as a beacon for detecting miR-301a expression. A-quenching efficiency up to 90% was achieved. The beacon we designed in this study can monitor the precise variation of miR-301a expression in vivo...
February 1, 2018: Journal of Nanoscience and Nanotechnology
Ning Wang, Yang Zhang, Huaxin Liang
The dysregulation of microRNAs (miRNAs) expression is closely related with tumorigenesis and tumour development in glioblastoma (GBM). In this study, we found that miRNA-598 (miR-598) expression was significantly downregulated in GBM tissues and cell lines. Restoring miR-598 expression inhibited cell proliferation and invasion in GBM. Moreover, we validated that metastasis associated in colon cancer-1 (MACC1) is a novel target of miR-598 in GBM. Recovered MACC1 expression reversed the inhibitory effects of miR-598 overexpression on GBM cells...
February 14, 2018: Oncology Research
Roshini Arivazhagan, Jaesuk Lee, Delger Bayarsaikhan, Peter Kwak, Myeongjoo Son, Kyunghee Byun, Ghasem Hosseini Salekdeh, Bonghee Lee
DNA Directed Polymerase Zeta Catalytic Subunit (REV3L) has recently emerged as an important oncogene. Although the expressions of REV3L are similar in normal and cancer cells, several mutations in REV3L have been shown to play important roles in cancer. These mutations cause proteins misfolding and mislocalization, which in turn alters their interactions and biological functions. miRNAs play important regulatory roles during the progression and metastasis of several human cancers. This study was undertaken to determine how changes in the location and interactions of REV3L regulate colon cancer progression...
January 12, 2018: Oncotarget
Yan Qin, Zhibin Huo, Xiang Song, Xiao Chen, Xiaopeng Tian, Xinjie Wang
Colorectal cancer (CRC) is a common digestive tract tumor. Cancer tissues and healthy tissues were extracted from patients with CRC who were treated at our hospital. Targetscan and PicTar were used to identify microRNAs (miRNAs/miRs) that may interact with phosphatase and tensin homolog deleted on chromosome ten (PTEN). Dual luciferase reporter assay was applied to detect whether the 3'-untranslated region (UTR) of PTEN was targeted by miR-106a. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) showed that there was significantly higher miR-106a expression level in cancer tissue compared with in healthy tissue...
March 2018: Oncology Letters
Xiaogang Shen, Xiaofei Zuo, Wenjin Zhang, Yifeng Bai, Xianpeng Qin, Nengyi Hou
MicroRNA (miR)-370 functions as a tumor suppressor or promoter in several cancers. However, the expression and biological role of miR-370 in colon cancer remains undefined. In the present study, miR-370 expression in both normal and malignant colon tissues was quantified by quantitative polymerase chain reaction. An in vitro cell viability and apoptosis assay and an in vitro xenograft tumor model were employed to investigate the role of miR-370 on colon cancer growth. Furthermore, the potential direct target of miR-370 was identified using a luciferase assay...
February 2018: Oncology Letters
Xiaofei Ning, Cong Wang, Meng Zhang, Kecheng Wang
Colon cancer is one of the most common cancers in the world. Epithelial-to-mesenchymal transition (EMT) is a crucial step in tumor progression and also involves in the acquisition of stem cell-like properties. Some miRNAs have been shown to function as either tumor suppressors or oncogenes in colon cancer. Here, we investigated the role of miR-147 in the regulation of stem cell-like traits of colon cancer cells. We observed that miR-147 was down-regulated in several colon cancer cell lines and overexpressed miR-147 decreased the expression of cancer stem cell (CSC) markers OCT4, SOX2 and NANOG in colon cancer cells (HCT116, SW480)...
February 9, 2018: Oncology Research
X-J Lai, H-F Cheng
OBJECTIVE: Ovarian cancer is one of the leading causes of cancer-related death in women, but treatment remained unsatisfactory. Studies have shown that lncRNA colon cancer-associated transcript 1 (CCAT1) plays an important regulatory role in different cancers, but its role in ovarian cancer remained largely unclear. PATIENTS AND METHODS: Quantitative Real-time polymerase chain reaction (qRT-PCR) was applied to detect the expression of lncRNA CCAT1 in ovarian cancer and adjacent tissue, and analysis was applied to explore the relationship between expression and clinical characteristic...
January 2018: European Review for Medical and Pharmacological Sciences
Zhiyao Xu, Jingjing Tao, Ping Chen, Long Chen, Sherven Sharma, Guanyu Wang, Qinghua Dong
SCOPE: Short chain fatty acid sodium butyrate (NaB) is the byproduct of bacterial anaerobic fermentation of dietary fiber in the colon, and has been shown to have an anti-tumor effect on colorectal cancer (CRC). The miR-200 family is a key regulator of the epithelial-mesenchymal transition (EMT). We investigated the role of miR-200s expression on cell migration in NaB treated CRC cells. METHODS AND RESULTS: HCT116 and LOVO CRC cells treated with NaB depicted reduced cell proliferation, enhanced apoptosis and cell cycle arrest...
February 8, 2018: Molecular Nutrition & Food Research
Andrew Fesler, Hua Liu, Jingfang Ju
Despite advances in colon cancer treatments, resistance and recurrence remain a significant challenge in treating patients. Novel therapeutic strategies are in urgent need to overcome resistance and improve patient outcomes. MicroRNA based therapeutics have potential to help combat resistance. In this study, we have shown that low miR-15a expression correlates with poor patient prognosis. We have demonstrated the therapeutic potential of miR-15a in colon cancer. miR-15a inhibits several important genes (BCL2, BMI1, YAP1 and DCLK1), decreasing cancer progression and resistance...
January 5, 2018: Oncotarget
Ping Gao, Miao He, Chunling Zhang, Changhui Geng
PURPOSE: The present study aimed to elucidate the pathogenesis of colon cancer and identify genes associated with tumor development. METHODS: Three datasets, two (GSE74602 and GSE44861) from the Gene Expression Omnibus database and RNA-Seq colon cancer data from The Cancer Genome Atlas data portal, were downloaded. These three datasets were grouped using a meta-analysis approach, and differentially expressed genes (DEGs) were identified between colon tumor samples and adjacent normal samples...
February 3, 2018: Gene
Liang Zhong, Jacques Huot, Martin J Simard
Extravasation of circulating cancer cells determines their metastatic potential. This process is initiated by the adhesion of cancer cells to vascular endothelial cells through specific interactions between endothelial adhesion receptors such as E-selectin and their ligands on cancer cells. In the present study, we show that miR-146a and miR-181b impede the expression of E-selectin by repressing the activity of its transcription factor NF-κB, thereby impairing the metastatic potentials of colon cancer cells by decreasing their adhesion to, and migration through, the endothelium...
February 5, 2018: Scientific Reports
(no author information available yet)
'EphB3-targeted regulation of miR-149 in the migration and invasion of human colonic carcinoma HCT116 and SW620 cells' by Guodong Zhang, Xiaozhu Liu, Yinfeng Li, Yan Wang, Huankun Liang, Kangyan Li, Laiqing Li, Cuicui Chen, Wenqiao Sun, Shoulei Ren, Pengfei Zhu and Licheng Zhang1 . The above article from Cancer Science, first published on 3 April 2017 in Wiley Online Library ( and in Volume 108, pp. 408-418, has been retracted by the journal Editor in Chief, Yusuke Nakamura, and John Wiley & Sons Australia, Ltd...
February 2018: Cancer Science
Ying Cui, Le-Gao Chen, Hai-Bo Yao, Jun Zhang, Ke-Feng Ding
Increasing evidence demonstrates that microRNAs (miRNAs/miRs), a type of non-coding small RNA, can regulate tumor cell migration, invasion and metastasis, and may therefore serve a major function in the occurrence and development of tumors. The present study investigated the effect of miR-383 on the proliferation, migration and invasion of colon cancer HT-29 and LoVo cell lines. The expression of miR-383 in colon cancer and adjacent non-tumor tissues was examined by reverse transcription-quantitative polymerase chain reaction...
January 2018: Oncology Letters
Sebastian Lamprecht, Markus Kaller, Eva Marina Schmidt, Cristina Blaj, Tobias S Schiergens, Jutta Engel, Andreas Jung, Heiko Hermeking, Thomas G P Grünewald, Thomas Kirchner, David Horst
PURPOSE: Colorectal cancers are composed of phenotypically different tumor cell subpopulations within the same core genetic background. Here, we identify high expression of the TALE transcription factor PBX3 in tumor cells undergoing epithelial-mesenchymal transition (EMT), analyze PBX3 regulation, and determine clinical associations in colorectal cancer. EXPERIMENTAL DESIGN: We used transcriptomic and in situ analyses to identify PBX3 expression in colorectal cancer, and cell biology approaches to determine its regulation and function...
February 1, 2018: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
Stephen J O'Brien, Jane V Carter, James F Burton, Brent G Oxford, Miranda N Schmidt, Jacob C Hallion, Susan Galandiuk
Colorectal cancer is associated with significant morbidity and mortality as many patients are diagnosed with advanced stage disease. MicroRNAs are small, non-coding RNA molecules which have a major role in gene expression regulation and are dysregulated in colorectal cancer. The miR-200 family is involved in epithelial-to-mesenchymal transition (EMT). This systematic review describes the roles of the miR-200 family in EMT in colorectal cancer. A search of electronic databases (PubMed and Embase) was conducted between January 2000 and July 2017...
January 31, 2018: International Journal of Cancer. Journal International du Cancer
Feng Shuai, Bo Wang, Shuxiao Dong
miR-522-3p is known to degrade bloom syndrome protein (BLM) and enhance expression of other proto-oncogenes leading totumorigenesis. This study aimed to investigate the molecular mechanisms of miR-522-3p in human colorectal cancer (CRC) cells. Expressions of miR-522-3p in CRC and adjacent tissues, as well as in normal human colon epithelial cell line (FHC) and five CRC cell lines were detected. Human CRC cell lines, HCT-116 and HT29, were transfected with miR-522-3p mimic, inhibitor or scrambled controls. Then, cell viability, apoptosis, cell-cycle progression, and the expressions of c-myc, cyclin E, CDK2, and BLM were respectively assessed...
January 31, 2018: Oncology Research
S Santasusagna, I Moreno, A Navarro, C Muñoz, F Martinez, R Hernández, J J Castellano, M Monzo
PURPOSE: Increasing evidence shows that altered metabolism is a critical hallmark in colon cancer. There is a strong need to explore the molecular mechanisms underlying cancer metabolism. Whether the aberrant expression of microRNAs contributes to cancer metabolism is not fully understood. miR-328 is a putative potential target of SLC2A1, but the regulating mechanism between them remains unknown. We have examined whether miR-328 directly regulates SLC2A1/GLUT1 expression in colon cancer cells...
January 26, 2018: Clinical & Translational Oncology
N Liu, F Jiang, X-Y Han, M Li, W-J Chen, Q-C Liu, C-X Liao, Y-F Lv
OBJECTIVE: To investigate the role of microRNA-155 (miR-155) in the potential invasion of colon cancer cell and the underlying mechanism. PATIENTS AND METHODS: The expression level of miR-155 in colon cancer and adjacent normal tissues was detected by Real-time quantitative polymerase chain reaction (RT-PCR). miR-155 mimics (miR-155), or siRNA against β-catenin (β-catenin siRNA), was transfected into human colon cancer cell line SW-480 using Lipofectamine 2000, respectively...
January 2018: European Review for Medical and Pharmacological Sciences
Kyung Hee Koo, Heechung Kwon
Depending on the function of their target genes, microRNAs (miRNAs) act as either tumor suppressors or oncogenes. Therefore, miRNAs represent a novel therapeutic strategy for prevention and management of cancer by targeting of onco-miRNAs or mimicking of tumor suppressor miRNAs. Herein, we identified novel tumor suppressor miRNAs and investigated their molecular mechanisms. To identify novel tumor suppressor miRNAs, we used 532 human miRNA mimic libraries and measured cell viability using MTS assays. The function of miR-4779 was then analyzed using cell cycle analyses and apoptosis, colony forming, and soft agar assays...
January 23, 2018: Cell Death & Disease
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