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https://www.readbyqxmd.com/read/29679755/long-non-coding-rna-xist-sponges-mir-34a-to-promotes-colon-cancer-progression-via-wnt-%C3%AE-catenin-signaling-pathway
#1
Ningning Sun, Guozun Zhang, Yingying Liu
Little is known about the role of long non-coding RNA XIST in the development of colon cancer. The aim of the present study was to investigate the levels of XIST in colon cancer, and explore its underlying mechanism. In this study, we found XIST expression level was upregulated in colon cancer tissues and cell lines. In addition, the growth rate of cells transfected with si-XIST was significantly decreased compared to that with si-NC, which was reversed by miR-34a targeted with 3'-UTR. Moreover, miR-34a suppressed the expression of WNT1 by binding with the 3'-UTR, which interact with WNT1 to inhibit the proliferation of cells...
April 18, 2018: Gene
https://www.readbyqxmd.com/read/29673421/long-noncoding-rna-linc01296-harbors-mir-21a-to-regulate-colon-carcinoma-proliferation-and-invasion
#2
Kecheng Wang, Meng Zhang, Cong Wang, Xiaofei Ning
Increasing evidences have demonstrated that aberrant expressions of long non-coding RNAs (lncRNAs) are closely correlated to various malignancies, as well as colon carcinoma (CC). The aim of this study was to investigate the role of lncRNA long intergenic noncoding RNA 001296 (LINC01296) in tumorigenesis of CC. The result of the quantitative real-time polymerase chain reaction (qRT-PCR) demonstrated that LINC01296 was upregulated in CC cancerous tissues and cell lines compared to adjacent normal tissue and normal liver cell lines...
April 19, 2018: Oncology Research
https://www.readbyqxmd.com/read/29670141/a-four-microrna-classifier-as-a-novel-prognostic-marker-for-tumor-recurrence-in-stage-ii-colon-cancer
#3
Havjin Jacob, Luka Stanisavljevic, Kristian Eeg Storli, Kjersti E Hestetun, Olav Dahl, Mette P Myklebust
About 20 percent of TNM-stage II colon cancer patients who are treated by surgical resection develop recurrence, and adjuvant chemotherapy in this group is still debated among researchers and clinicians. Currently, adverse histopathological and clinical factors are used to select patients for adjuvant chemotherapy following surgery. However, additional biomarkers to classify patients at risk of recurrence are needed. We have conducted a study using fresh frozen tumor tissue from 54 TNM-stage II colon cancer patients and performed microRNA profiling using next-generation sequencing...
April 18, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29669684/impact-on-surgical-and-oncological-results-of-the-use-of-colonic-stents-as-a-bridge-to-surgery-for-potentially-curable-occlusive-colorectal-neoplasms
#4
Antònia Crespí-Mir, Juan Manuel Romero-Marcos, Anabel de la Llave-Serralvo, Carlos Dolz-Abadía, José Andrés Cifuentes-Ródenas
INTRODUCTION: The outcomes of patients treated with colonic stents as a bridge to surgery (BTS) have recently been questioned in terms of safety and long-term oncologic outcomes. The aim of this study is to evaluate the effects on surgical and oncologic outcomes of colonic stents as a BTS for potentially resectable obstructive colorectal cancer. METHODS: We conducted a retrospective analysis of patients operated on for potentially resectable obstructive colorectal cancer with or without distant disease between September 2002 and October 2015, comparing the patients treated with a colonic stent as a BTS (Stent group) with those directly operated on (Surgery group)...
April 15, 2018: Cirugía Española
https://www.readbyqxmd.com/read/29660302/mir-223-rhob-signaling-pathway-regulates-the-proliferation-and-apoptosis-of-colon-adenocarcinoma
#5
Li-Juan Wei, Jun-An Li, Dong-Mei Bai, Ying Song
MicroRNAs (miRNAs) can function as tumor suppressor or oncogenic genes. The putative targets of miR-223 include tumor suppressor gene, RhoB. Here we sought to investigate the role of miR-223-RhoB signaling pathway in proliferation of colon cancer. We used Western blot, immunofluorescence staining, or RT-PCR to detect expression levels of miR-223 and RhoB in colon adenocarcinoma and adjacent non-cancerous tissue samples, or in human colon adenocarcinoma cell lines. MTT assay was used to determine proliferation and apoptosis in cell lines...
April 13, 2018: Chemico-biological Interactions
https://www.readbyqxmd.com/read/29656186/dclk1-plays-an-important-role-in-colorectal-cancer-tumorgenesis-through-the-regulation-of-mir-200c
#6
Yasaman Mohammadi, Seyed Mohammad Tavangar, Massoud Saidijam, Razieh Amini, Katayoon Etemadi, Fatemeh Karimi Dermani, Rezvan Najafi
Doublecortin-like kinase 1 (DCLK1) is a protein kinase that is known as a specific cancer stem cell (CSC) marker in colorectal cancer (CRC). Deregulation of DCLK1 expression has been reported in various cancers. We measured the protein expression of DCLK1 in 38 CRC and normal colon samples by immunohistochemistry (IHC). HCT-116 and SW-48 cells were transfected with DCLK1 siRNA and analyzed for expression of DCLK1 and miR-200c. The effects of DCLK1 knockdown on cell migration, invasion, sphere-forming, and apoptosis were explored...
April 12, 2018: Biomedicine & Pharmacotherapy, Biomédecine & Pharmacothérapie
https://www.readbyqxmd.com/read/29620230/formononetin-inhibits-colon-carcinoma-cell-growth-and-invasion-by-microrna%C3%A2-149%C3%A2-mediated-ephb3-downregulation-and-inhibition-of-pi3k-akt-and-stat3-signaling-pathways
#7
Ai-Lei Wang, Yong Li, Qun Zhao, Li-Qiao Fan
Formononetin (Form), a phytoestrogen extracted from the roots of Astragalus membranaceus, is one of the fundamental herbs used in traditional Chinese medicine because of its protective effects against certain malignant tumors. However, its role in colon carcinoma cells and the underlying molecular mechanisms have not been completely elucidated. The present study aimed to demonstrate that Form significantly inhibited the proliferation and invasion of the colon carcinoma cell lines SW1116 and HCT116. Mechanistic studies have suggested that Form suppresses colon carcinoma cell growth by downregulating cell cycle‑associated protein (cyclin D1) expression and arresting the cell cycle at the G0‑G1 checkpoint...
April 5, 2018: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29615842/mir-216b-suppresses-colorectal-cancer-proliferation-migration-and-invasion-by-targeting-srpk1
#8
Yanfen Yao, Qiaorong Li, Hong Wang
Background: MiR-216b has been reported to be involved in the development of some cancers, however, the role of miR-216b in colorectal cancer (CRC) remains unclear. Purpose: This study aimed to investigate the mechanism underlying miR-216b-induced CRC development. Methods: We detected the expression of miR-216b in 80 cases of CRC tissues and cell lines, and further analyzed the association between miR-216b and clinical pathological indicators as well as prognosis...
2018: OncoTargets and Therapy
https://www.readbyqxmd.com/read/29600711/jq-1-inhibits-colon-cancer-proliferation-via-suppressing-wnt-%C3%AE-catenin-signaling-and-mir-21
#9
Yan Zhang, Suli Tian, Jidong Xiong, Yongxu Zhou, Hongyu Song, Chang Liu
Bromodoamin and extraterminal (BET) protein inhibitors are a novel class of cancer therapeutics. Here we aim to investigate the efficacy and mechanism of JQ-1, a potent BET inhibitor, in colon cancer therapy. JQ-1 was used to treat SW480 colon cancer mouse xenografts. Tumor size and mouse survival were recorded. Cell apoptosis was evaluated by Annex V-FIC/PI flow cytometry. ChIP-q-PCR analysis was used to assess the H3K27 trimethylation (H3K27m3) of the p16 promoter. Wnt signaling was evaluated by Nkd2 and β-catenin levels...
March 30, 2018: Chemical Research in Toxicology
https://www.readbyqxmd.com/read/29589310/rt-qpcr-for-fecal-mature-microrna-quantification-and-validation
#10
Farid E Ahmed, Nancy C Ahmed, Mostafa M Gouda, Paul W Vos, Chris Bonnerup
By routinely and systematically being able to perform quantitative stem-loop reverse transcriptase (RT) followed by TaqMan® minor-groove binding (MGB) probe, real-time quantitative PCR analysis on exfoliated enriched colonocytes in stool, using human (Homo sapiens, hsa) micro(mi)RNAs to monitor changes of their expression at various stages of colorectal (CRC) progression, this method allows for the reliable and quantitative diagnostic screening of colon cancer (CC). Although the expression of some miRNA genes tested in tissue shows less variability in normal or cancerous patients than in stool, the noninvasive stool by itself is well suited for CC screening...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29588449/confirmation-of-a-metastasis-specific-microrna-signature-in-primary-colon-cancer
#11
Robert R J Coebergh van den Braak, Anieta M Sieuwerts, Zarina S Lalmahomed, Marcel Smid, Saskia M Wilting, Sandra I Bril, Shanshan Xiang, Michelle van der Vlugt-Daane, Vanja de Weerd, Anne van Galen, Katharina Biermann, J Han J M van Krieken, Wigard P Kloosterman, John A Foekens, John W M Martens, Jan N M IJzermans
The identification of patients with high-risk stage II colon cancer who may benefit from adjuvant therapy may allow the clinical approach to be tailored for these patients based on an understanding of tumour biology. MicroRNAs have been proposed as markers of the prognosis or treatment response in colorectal cancer. Recently, a 2-microRNA signature (let-7i and miR-10b) was proposed to identify colorectal cancer patients at risk of developing distant metastasis. We assessed the prognostic value of this signature and additional candidate microRNAs in an independent, clinically well-defined, prospectively collected cohort of primary colon cancer patients including stage I-II colon cancer without and stage III colon cancer with adjuvant treatment...
March 27, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29564000/berberine-regulates-the-microrna-21-itg%C3%AE-4-pdcd4-axis-and-inhibits-colon-cancer-viability
#12
Yanfeng Lü, Bingbing Han, Hualong Yu, Zhenghua Cui, Zhiwen Li, Jianxin Wang
Berberine is sourced from multiple medicinal herb resources and is easy to extract. With the advantages of low price, safety and convenience, berberine may have potential for wide clinical use. The present study aimed to investigate whether berberine inhibited the viability of colon cancer and whether it regulated the three-gene network microRNA (miR)-21-integrin β4 (ITGβ4)-programmed cell death 4 (PDCD4). It was demonstrated that berberine treatment suppressed colon cancer cell viability, and induced apoptosis and activated caspase-3 activity in the human colon carcinoma HCT116 cell line...
April 2018: Oncology Letters
https://www.readbyqxmd.com/read/29560130/stromal-mir-20a-controls-paracrine-cxcl8-secretion-in-colitis-and-colon-cancer
#13
Steven A Signs, Robert C Fisher, Uyen Tran, Susmita Chakrabarti, Samaneh K Sarvestani, Shao Xiang, David Liska, Veronique Roche, Wei Lai, Haley R Gittleman, Oliver Wessely, Emina H Huang
Inflammatory bowel disease (IBD) affects one million people in the US. Ulcerative colitis (UC) is a subtype of IBD that can lead to colitis-associated cancer (CAC). In UC, the rate of CAC is 3-5-fold greater than the rate of sporadic colorectal cancer (CRC). The pathogenesis of UC and CAC are due to aberrant interactions between host immune system and microenvironment, but precise mechanisms are still unknown. In colitis and CAC, microenvironmental fibroblasts exhibit an activated, inflammatory phenotype that contributes to tumorigenesis accompanied by excessive secretion of the chemokine CXCL8...
February 27, 2018: Oncotarget
https://www.readbyqxmd.com/read/29556386/role-of-micrornas-in-the-resistance-of-colorectal-cancer-to-chemoradiotherapy
#14
REVIEW
Qi-Bing Wu, Xin Sheng, Ning Zhang, Ming-Wei Yang, Fan Wang
Colorectal cancer (CRC) is among the main tumor-related causes of death worldwide. The fact that the majority of the patients develop resistance to chemoradiotherapy (CRT) is a major obstacle for the treatment of CRC. In order to develop more effective treatment strategies, it is crucial to elucidate the mechanisms underlying the development of resistance to CRT. Several studies have recently indicated the regulatory effects of microRNAs (miRNAs) in response to antitumor agents. For example, miR-34a attenuates the chemoresistance of colon cancer to 5-FU by inhibiting E2F3 and SIRT1...
April 2018: Molecular and Clinical Oncology
https://www.readbyqxmd.com/read/29552759/long-non-coding-rna-pvt1-functions-as-an-oncogene-in-human-colon-cancer-through-mir-30d-5p-runx2-axis
#15
Xiuguo Yu, Jianpei Zhao, Yaguang He
PURPOSE: Recently, long noncoding RNAs (lncRNAs) have caught more attention for their role in tumor progression. Colon cancer is one of these ordinary malignant tumors. This study aimed to identify how lnc RNA PVT1 affects the progression of colon cancer. METHODS: PVT1 expression of both colon cancer cell tissue and 60 paired cancer and peri-tumoral tissue samples was detected by real-time quantitative polymerase chain reaction (RT-qPCR). The associations between lnc RNA PVT1 expression level and clinicopathological characteristics and patients' disease-free survival rate were evaluated...
January 2018: Journal of B.U.ON.: Official Journal of the Balkan Union of Oncology
https://www.readbyqxmd.com/read/29552117/microrna-155-increases-colon-cancer-chemoresistance-to-cisplatin-by-targeting-forkhead-box-o3
#16
Yuewen Gao, Zhaoyan Liu, Zhaohong Ding, Shicai Hou, Jun Li, Kehua Jiang
To investigate the effect of microRNA (miR)-155 on colon cancer chemoresistance to cisplatine and its mechanism. Reverse transcription quantitative polymerase chain reaction was used to measure the levels of miR-155 and forkhead box O3 (FOXO3) in colon cancer specimens and cell lines. Overexpression of miR-155 and miR-155 inhibitor were transfected into colon cancer cell lines to investigate its role of chemoresistance to cisplatin in colon cancer. MTS assays were used to analyse cell viability in vitro . In vivo tumor formation assays were performed in C57BL/6 wild type and miR-155 knockout mice (miR-155-/-)...
April 2018: Oncology Letters
https://www.readbyqxmd.com/read/29545333/a-mir-29b-byproduct-sequence-exhibits-potent-tumor-suppressive-activities-via-inhibition-of-nf-%C3%AE%C2%BAb-signaling-in-kras-mutant-colon-cancer-cells
#17
Akira Inoue, Tsunekazu Mizushima, Xin Wu, Daisuke Okuzaki, Nanami Kambara, Sho Ishikawa, Jiaqi Wang, Yamin Qian, Haruka Hirose, Yuhki Yokoyama, Ryo Ikeshima, Masayuki Hiraki, Norikatsu Miyoshi, Hidekazu Takahashi, Naotsugu Haraguchi, Taishi Hata, Chu Matsuda, Yuichiro Doki, Masaki Mori, Hirofumi Yamamoto
We previously demonstrated that miR-29b-3p is a hopeful microRNA (miRNA)-based therapies against colorectal cancer (CRC). In this study, we aimed to clarify a value of miR-29b-1-5p as a next generation treatment, especially for KRAS mutant CRC. RT-PCR assay showed that expression of miR-29b-3p was high and its partner strand, miR-29b-1-5p level was only negligible in clinical CRC samples. Mimic-miR-29b-1-5p significantly inhibited proliferation of KRAS mutant CRC cell lines DLD1 and SW480 and KRAS wild type HT29 cells...
March 15, 2018: Molecular Cancer Therapeutics
https://www.readbyqxmd.com/read/29512763/studying-the-mechanism-of-plagl2-overexpression-and-its-carcinogenic-characteristics-based-on-3-untranslated-region-in-colorectal-cancer
#18
Chen Su, Daojiang Li, Nanpeng Li, Yuheng Du, Chunxing Yang, Yang Bai, Changwei Lin, Xiaorong Li, Yi Zhang
Pleomorphic adenoma gene like-2 (PLAGL2) is a zinc finger protein transcription factor, which is upregulated and serves an oncogenic function in multiple human malignancies, including colorectal cancer (CRC). First, reverse transcription-quantitative polymerase chain reaction (RT-qPCR) was used to detect the expression levels of PLAGL2 in CRC tissues and normal tissues. Then, bioinformatics analysis, RT-qPCR, western blotting, luciferase reporter assays and RNA-binding protein immunoprecipitation assays were performed to explore whether the underlying mechanisms, including copy number variation (CNV), microRNAs (miRNAs/miRs) and RNA-binding proteins (RBPs) led to the abnormal expression of PLAGL2...
March 6, 2018: International Journal of Oncology
https://www.readbyqxmd.com/read/29512698/downregulation-of-sirt6-by-mir-34c-5p-is-associated-with-poor-prognosis-and-promotes-colon-cancer-proliferation-through-inhibiting-apoptosis-via-the-jak2-stat3-signaling-pathway
#19
Ning Li, Dong Mao, Yansha Cao, Hua Li, Fu Ren, Keyan Li
Sirtuin 6 (SIRT6) is a member of the nicotinamide adenine dinucleotide positivity-dependent class III deacetylase sirtuin family. The present study aimed to explore the expression and function of SIRT6 in colon cancer. Furthermore, the partial mechanism underlying the dysregulation of SIRT6 was investigated. The results of immunohistochemistry demonstrated that SIRT6 was markedly downregulated in colon cancer tissues, and patients with high SIRT6 expression had a better prognosis than those who did not. The proliferation and apoptotic assays demonstrated that SIRT6 was able to suppress colon cancer cell proliferation and induce apoptosis via the Janus kinase 2 (JAK2)/signal transducer and activator of transcription 3 (STAT3) signaling pathway...
March 5, 2018: International Journal of Oncology
https://www.readbyqxmd.com/read/29511442/srpx2-regulates-colon-cancer-cell-metabolism-by-mir-192-215-via-pi3k-akt
#20
Jian Zhao, Jian Xu, Rui Zhang
Colon cancer is one of common cancer in the world and glycolysis is one of the major problems in colon cancer therapy. MicroRNAs (miRNAs) involve in colon cancer progression. Sushi repeat-containing protein X-linked 2 (SRPX2) is associated with poor prognosis in some cancer patients, however, the role of SRPX2 including glycolytic metabolism regulated by miRNAs is unclear in colon cancer. So, the purpose of the present study is to elucidate the underlying mechanism in colon cancer metabolism mediated by SRPX2...
2018: American Journal of Translational Research
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