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https://www.readbyqxmd.com/read/28646161/circulating-exosomal-mir-125a-3p-as-a-novel-biomarker-for-early-stage-colon-cancer
#1
Jing Wang, Feihu Yan, Qi Zhao, Fei Zhan, Ruitao Wang, Liang Wang, Yanqiao Zhang, Xiaoyi Huang
Circulating exosome holds great potentials as biomarker for diagnosis and prognosis of human cancers. Previously, we have applied small RNA sequencing to identify aberrantly expressed exosomal miRNAs as candidates for diagnostic markers in colon cancer patients. In this validation cohort, plasma derived exosomal miRNA was isolated from 50 early-stage colon cancer patients and 50 matched healthy volunteers. Real-time qRT-PCR revealed that miR-125a-3p, miR-320c were significantly up-regulated in plasma exosomes of the patients with early stage colon cancer...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28646148/mir-15b-5p-resensitizes-colon-cancer-cells-to-5-fluorouracil-by-promoting-apoptosis-via-the-nf-%C3%AE%C2%BAb-xiap-axis
#2
Ci Zhao, Qi Zhao, Chunhui Zhang, Guangyu Wang, Yuanfei Yao, Xiaoyi Huang, Fei Zhan, Yuanyuan Zhu, Jiaqi Shi, Jianan Chen, Feihu Yan, Yanqiao Zhang
Drug resistance, which is closely correlated with an imbalance in apoptosis, endows colorectal cancer (CRC) with enhanced progression capacity irrespective of the treatment with therapeutics. We report that miR-15b-5p is a tumor suppressor whose level is globally decreased in CRC cells and tissues. Over-expression of miR-15b-5p not only promoted 5-fluorouracil (5-FU)-induced cellular apoptosis but also reversed the chemoresistance of 5-FU in vitro and in vivo. As a key mediator of inflammation-induced cancer, miR-15b-5p enhances these therapeutic effects are mainly attributed to targeting of the NF-κB signaling pathway through negative regulation of NF-κB1 and one of its kinase complexes IKK-α...
June 23, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28631575/long-non-coding-rna-ccat1-mir-218-zfx-axis-modulates-the-progression-of-laryngeal-squamous-cell-cancer
#3
Yaming Zhang, Haili Hu
Long non-coding RNAs have been proved to be closely associated with different cancers. This study was designed to elucidate the function and mechanisms of colon cancer-associated transcript-1 in the progression of human laryngeal squamous cell cancer. Expressions of colon cancer-associated transcript-1, microRNA-218, and zinc finger protein, X-linked messenger RNA were measured using quantitative real-time polymerase chain reaction, and the expression level of zinc finger protein, X-linked protein was detected using western blot...
June 2017: Tumour Biology: the Journal of the International Society for Oncodevelopmental Biology and Medicine
https://www.readbyqxmd.com/read/28629469/a-putative-molecular-network-associated-with-colon-cancer-metastasis-constructed-from-microarray-data
#4
Songtao Chu, Haipeng Wang, Miao Yu
BACKGROUND: This study aimed to identify the potential molecular network associated with colon cancer metastasis. METHODS: A gene expression profile dataset (GSE40367) downloaded from Gene Expression Omnibus was used to identify and compare differentially expressed genes (DEGs) between primary colon adenocarcinoma tissues and matched tissue samples of liver metastases of colon adenocarcinoma. After the functional analysis of the DEGs, their protein-protein interactions (PPIs) were analyzed, and the transcription factors (TFs) and microRNAs (miRNAs) that regulated these DEGs were predicted...
June 19, 2017: World Journal of Surgical Oncology
https://www.readbyqxmd.com/read/28624221/mir-1290-is-a-biomarker-in-dna-mismatch-repair-deficient-colon-cancer-and-promotes-resistance-to-5-fluorouracil-by-directly-targeting-hmsh2
#5
Ling Ye, Tao Jiang, Huanzhang Shao, Lin Zhong, Zhaowen Wang, Yuan Liu, Huamei Tang, Bingyu Qin, Xiaoqing Zhang, Junwei Fan
5-Fluorouracil (5FU)-based adjuvant therapy is the first-line therapy for treating stage II and III colon cancer after surgery. However, its therapeutic efficacy is limited because of chemoresistance, especially in deficient mismatch repair (dMMR) colon cancer. Here, we first used laser capture microdissection to obtain purified cells from four dMMR and four proficient mismatch repair (pMMR) colon cancer tissues. Second, microRNA (miRNA) microarray chips were used to identify miRNAs that are differentially expressed between these two classes of tumors...
June 16, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28617555/correlation-analysis-of-mir-200b-mir-200c-and-mir-141-with-liver-metastases-in-colorectal-cancer-patients
#6
S-H Zhu, X-C He, L Wang
OBJECTIVE: To investigate correlation relationship between serum miR-200b, miR-200c, miR-141 levels with liver metastases in colorectal cancer patients. PATIENTS AND METHODS: A total of 85 colorectal cancer patients with liver metastases and 54 colorectal cancer patients without liver metastases were divided into experimental group and control. Serum sample was collected before surgery and tested by Real-time PCR to evaluate miR-200b, miR-200c, miR-141 expressions...
May 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28600480/mir-193a-3p-is-a-key-tumor-suppressor-in-ulcerative-colitis-associated-colon-cancer-and-promotes-carcinogenesis-through-up-regulation-of-il17rd
#7
Joel Pekow, Katherine Meckel, Urszula Dougherty, Yong Huang, Xindi Chen, Anas Almoghrabi, Reba Mustafi, Fatma Ayaloglu-Butun, Zifeng Deng, Haider I Haider, John Hart, David T Rubin, John H Kwon, Marc Bissonnette
Purpose: Patients with ulcerative colitis (UC) are at increased risk for colorectal cancer, although mechanisms underlying neoplastic transformation are poorly understood. We sought to evaluate the role of microRNAs in neoplasia development in this high-risk population.   <p>Experimental Design: Tissue from 12 controls, 9 UC patients without neoplasia, and 11 UC patients with neoplasia was analyzed. miRNA array analysis was performed and select miRNAs assayed by real-time PCR on the discovery cohort and a validation cohort...
June 9, 2017: Clinical Cancer Research: An Official Journal of the American Association for Cancer Research
https://www.readbyqxmd.com/read/28600172/conditional-knockout-of-microrna-31-promotes-the-development-of-colitis-associated-cancer
#8
Zhaoyuan Liu, Jing Bai, Lingyun Zhang, Fangzhou Lou, Fang Ke, Wei Cai, Honglin Wang
MicroRNA-31 (miR-31) is an evolutionarily conserved microRNA, and its biological function in colorectal cancer and other cancers is controversial. In this study, we identified the host gene of mouse miR-31 and found that miR-31 was over-expressed in both human colorectal cancer and mouse colon cancer models. We here developed a miR-31 conditional knockout mouse model that allows for colon epithelium specific deletion of miR-31 to investigate its functionality in colon cancer development. We demonstrated that mice with miR-31 conditional deletion resulted in more severe colitis-associated cancer than wild-type, and we further identified Wdr5 as an important target of miR-31...
June 6, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/28599301/a-functional-variant-in-grem1-confers-risk-for-colorectal-cancer-by-disrupting-a-hsa-mir-185-3p-binding-site
#9
Jiaoyuan Li, Hui Liu, Li Zou, Juntao Ke, Yi Zhang, Ying Zhu, Yang Yang, Yajie Gong, Jianbo Tian, Danyi Zou, Xiating Peng, Jing Gong, Rong Zhong, Kun Huang, Jiang Chang, Xiaoping Miao
The transforming growth factor beta (TGF-β) pathway has been implicated in carcinogenesis of intestinal canal. Except for common variants indentified by genome-wide association studies, variants with lower frequency can also explain a part of the disease heritability, especially those in gene regulatory regions. In this study, we searched for colorectal cancer (CRC) related functional low-frequency variants (minor allele frequency 1-5%) in untranslated regions (UTR) involved in the TGF-β signaling using a next-generation sequencing based approach...
May 23, 2017: Oncotarget
https://www.readbyqxmd.com/read/28594651/mir-4728-3p-functions-as-a-tumor-suppressor-in-ulcerative-colitis-associated-colorectal-neoplasia-through-regulation-of-focal-adhesion-signaling
#10
Joel Pekow, Alan L Hutchison, Katherine Meckel, Kymberly Harrington, Zifeng Deng, Nitya Talasila, David T Rubin, Stephen B Hanauer, Roger Hurst, Konstantin Umanskiy, Alessandro Fichera, John Hart, Aaron R Dinner, Marc Bissonnette
BACKGROUND: As mechanisms of neoplasia in patients with ulcerative colitis (UC) remain poorly understood, we sought to identify pathways of carcinogenesis in this high-risk population. METHODS: MicroRNA (miRNA) and mRNA expression was examined in nondysplastic rectosigmoid mucosa from UC patients with (n = 19) or without remote colon neoplasia (n = 23). We developed a method to identify miRNA-regulated pathways based on differentially expressed miRNAs and their putative mRNAs targets in the same samples...
June 9, 2017: Inflammatory Bowel Diseases
https://www.readbyqxmd.com/read/28591941/-study-on-the-effect-of-overexpression-of-mir-18a-on-cellular-proliferation-and-migration-by-targeting-atm-in-human-colorectal-cancer-cells
#11
Ming-Jia Liu, Li-Hong Chen, Kai-Feng Hu, Xiao-Long Yang, Jing-Ying Dong, Ji Liu
OBJECTIVES: To study the regulation to colon cancer cellular biological properties through miR-18a targeting ataxia-telangiectasia mutated gene (ATM). METHODS: A target of miR-18a was predicted by using bioinformatics tools. The miR-18a mimics and inhibitors were designed and synthesized. The expression of endogenous miR-18a in colon cancer cell line HCT116 was up-regulated or down-regulated by transfection. The effect of overexpression of miR-18a on cellular proliferation, invasion and migration via regulation of ATM gene expression was confirmed in vitro by using qRT-PCR, Western blot, MTT assay, clone forming assay and Transwell method, respectively...
July 2016: Sichuan da Xue Xue Bao. Yi Xue Ban, Journal of Sichuan University. Medical Science Edition
https://www.readbyqxmd.com/read/28571588/expression-profiling-indicating-low-selenium-sensitive-microrna-levels-linked-to-cell-cycle-and-cell-stress-response-pathways-in-the-caco-2-cell-line
#12
Mark J McCann, Kunjana Rotjanapun, John E Hesketh, Nicole C Roy
Se is an essential micronutrient for human health, and fluctuations in Se levels and the potential cellular dysfunction associated with it may increase the risk for disease. Although Se has been shown to influence several biological pathways important in health, little is known about the effect of Se on the expression of microRNA (miRNA) molecules regulating these pathways. To explore the potential role of Se-sensitive miRNA in regulating pathways linked with colon cancer, we profiled the expression of 800 miRNA in the CaCo-2 human adenocarcinoma cell line in response to a low-Se (72 h at <40 nm) environment using nCounter direct quantification...
June 2, 2017: British Journal of Nutrition
https://www.readbyqxmd.com/read/28567416/negative-correlation-between-mir-200c-and-decorin-plays-an-important-role-in-the-pathogenesis-of-colorectal-carcinoma
#13
Ren-Yi Tang, Zun Wang, Hong-Qi Chen, Si-Bo Zhu
Aim. To demonstrate the regulatory role of miRNA in colorectal carcinoma (CRC) and reveal the transcript markers that may be associated with CRC clinical outcomes. Method. Herein, we analyzed both mRNA and miRNA gene expression profiles of 255 CRC tumor samples from The Cancer Genome Atlas project to reveal the regulatory association between miRNA and mRNA. Also, the potential role of gene coexpression network in CRC has been explored. Results. The negative correlation between miR-200c and DCN (Decorin) was calculated in CRC, indicating that DCN could be a potential target of miR-200c...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28560511/caveolin-1-is-markedly-downregulated-in-patients-with-early-stage-colorectal-cancer
#14
Blanca Torrejón, Ion Cristóbal, Federico Rojo, Jesús García-Foncillas
BACKGROUND: Caveolin-1 (CAV-1), the main scaffold protein in caveolae, is frequently deregulated in human cancer. Of importance, this protein has been described to show tumor suppressor or oncogenic properties depending on the cell type and the stage of the disease. In fact, its role in colorectal cancer (CRC) remains to be fully clarified due to discrepancies in the literature. METHODS: We analyzed CAV-1 by western blot in a set of early-stage CRC patients with paired tumor tissue and normal colonic mucosa available...
May 30, 2017: World Journal of Surgery
https://www.readbyqxmd.com/read/28560419/upregulation-of-mir-101-enhances-the-cytotoxic-effect-of-anticancer-drugs-through-inhibition-of-colon-cancer-cell-proliferation
#15
Le-Gao Chen, Ying-Jie Xia, Ying Cui
This study investigated the effect of miR-101 on proliferation, migration, invasion, and chemotherapy sensitivity in colon cancer cell lines HT-29 and RKO. MicroRNAs are a class of small noncoding RNA molecules, which play important roles in diverse biological processes of human cancers, such as carcinogenesis, development, differentiation, and apoptosis. The expression of miR-101 in colon cancer and adjacent non-tumor tissues were examined by quantitative real-time polymerase chain reaction. The expression of miR-101 was upregulated by recombinant adenovirus Ad-miR-101...
May 24, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28552992/microrna-200b-is-downregulated-in-colon-cancer-budding-cells
#16
Kirsten Nguyen Knudsen, Jan Lindebjerg, Boye Schnack Nielsen, Torben Frøstrup Hansen, Flemming Brandt Sørensen
BACKGROUND: The microRNA-200 (miR-200) family acts as a major suppressor of epithelial-mesenchymal transition (EMT). Impaired miR-200 expression may lead to EMT initiation and eventually cancer dissemination. The presence of tumor budding cells (TBC) is associated with metastasis and poor prognosis, and molecular similarities to EMT indicate that these cells may reflect ongoing EMT. The aim of this study was to investigate the expression of miR-200b in budding cells of colon cancer and the relationship with the EMT-markers E-cadherin, β-catenin and laminin-5γ2...
2017: PloS One
https://www.readbyqxmd.com/read/28550684/long-noncoding-rna-ccat2-knockdown-suppresses-tumorous-progression-by-sponging-mir-424-in-epithelial-ovarian-vancer
#17
Fu Hua, Chang-Hua Li, Xiao-Gang Chen, Xiao-Ping Liu
Epithelial ovarian cancer (EOC) is the one of most common gynecological malignant tumor with highest mortality, meanwhile, series of lncRNAs have been validated to play vital role in EOC tumorigenesis. Colon cancer-associated transcript 2 (CCAT2) has been verified as oncogenic lncRNA in multiple tumors, however, the role of CCAT2 in EOC genesis is still unclear. The purpose of present study is to probe the function of CCAT2 on EOC. Preliminary experiments found that CCAT2 expression was significantly up-regulated in EOC tissue and cell lines compared to noncancerous tissue and cells...
May 21, 2017: Oncology Research
https://www.readbyqxmd.com/read/28549913/microrna-186-5p-overexpression-modulates-colon-cancer-growth-by-repressing-the-expression-of-the-fam134b-tumour-inhibitor
#18
Farhadul Islam, Vinod Gopalan, Jelena Vider, Riajul Wahab, Faeza Ebrahimi, Cu-Tai Lu, Kais Kasem, Alfred K Y Lam
OBJECTIVES: The role and underlying mechanism of miR-186-5p in colorectal cancer remain unknown. The present study aims to examine the various cellular effects of miR-186-5p in the carcinogenesis of colorectal cancer. Also, the interacting targets and association of clinicopathological factors with miR-186-5p expression in patients with colorectal cancer were analysed. METHODS: The miR-186-5p expression levels in colorectal cancer tissues (n=126) and colon cancer cell lines (n=3) were analysed by real-time PCR...
May 24, 2017: Experimental Cell Research
https://www.readbyqxmd.com/read/28549468/microrna-608-inhibits-proliferation-of-bladder-cancer-via-akt-foxo3a-signaling-pathway
#19
Zhen Liang, Xiao Wang, Xin Xu, Bo Xie, Alin Ji, Shuai Meng, Shiqi Li, Yi Zhu, Jian Wu, Zhenghui Hu, Yiwei Lin, Xiangyi Zheng, Liping Xie, Ben Liu
BACKGROUND: Current evidence indicates that miR-608 is widely down-regulated in various malignant tumors including liver cancer, colon cancer, lung cancer and glioma, and acts as a tumor suppressor by inhibiting cell proliferation, invasion and migration or by promoting apoptosis. The specific biological function of miR-608 in bladder cancer is still unknown. METHODS: qRT-PCR and Chromogenic in Situ Hybridization (CISH) was conducted to assess the expression of miR-608 in paired BCa tissues and adjacent non-tumor bladder urothelial tissues...
May 26, 2017: Molecular Cancer
https://www.readbyqxmd.com/read/28549102/long-non-coding-rna-ccat1-mir-148a-axis-promotes-osteosarcoma-proliferation-and-migration-through-regulating-pik3ip1
#20
Jingwei Zhao, Liming Cheng
Osteosarcoma is the most frequent type of malignant primary bone tumor. Although many efforts have been made, the survival rate of osteosarcoma still remains unsatisfied. Long non-coding RNAs (lncRNAs) have been demonstrated to be associated with many diseases including tumors, and involved in the regulation of a wide array of pathophysiological processes. Colon-cancer-associated transcript-1 (CCAT1) was first identified in colon cancer and has subsequently been reported to perform many functions in tumor progression...
June 1, 2017: Acta Biochimica et Biophysica Sinica
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