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https://www.readbyqxmd.com/read/28098901/effect-of-evodiamine-and-berberine-on-the-interaction-between-dnmts-and-target-micrornas-during-malignant-transformation-of-the-colon-by-tgf-%C3%AE-1
#1
Chao Huang, Hong Liu, Xiu-Li Gong, Li-Yun Wu, Bin Wen
The tissue microenvironment functions as a crucial player in carcinogenesis, and transforming growth factor-β1 (TGF-β1) within the microenvironment stimulates the formation of neoplasms. Using an in vitro model of malignancy induced by TGF-β1, we assessed the effect of evodiamine and berberine on the interaction between DNA methyltransferases (DNMTs) and target microRNAs (miRNAs) in the model. Colon tissues from neonatal rats 7 days of age were cultured and malignancy was induced by TGF-β1 in vitro for 48 h, and then the tissues were respectively treated with evodiamine and berberine for 24 h...
January 17, 2017: Oncology Reports
https://www.readbyqxmd.com/read/28061765/mir-663a-regulates-growth-of-colon-cancer-cells-after-administration-of-antimicrobial-peptides-by-targeting-cxcr4-p21-pathway
#2
Kengo Kuroda, Tomokazu Fukuda, Marija Krstic-Demonacos, Constantinos Demonacos, Kazuhiko Okumura, Hiroshi Isogai, Miwa Hayashi, Kazuki Saito, Emiko Isogai
BACKGROUND: Antimicrobial peptides (AMPs) play important roles in the innate immune system of all life forms and recently have been characterized as multifunctional peptides that have a variety of biological roles such as anticancer agents. However, detailed mechanism of antimicrobial peptides on cancer cells is still largely unknown. METHODS: miRNA array and real-time qPCR were performed to reveal the behavior of miRNA in colon cancer HCT116 cells during the growth suppression induced by the AMPs...
January 7, 2017: BMC Cancer
https://www.readbyqxmd.com/read/28061476/mir-675-5p-supports-hypoxia-induced-epithelial-to-mesenchymal-transition-in-colon-cancer-cells
#3
Viviana Costa, Alessia Lo Dico, Aroldo Rizzo, Francesca Rajata, Marco Tripodi, Riccardo Alessandro, Alice Conigliaro
The survival rates in colon cancer patients are inversely proportional to the number of lymph node metastases. The hypoxia-induced Epithelial to Mesenchymal Transition (EMT), driven by HIF1α, is known to be involved in cancer progression and metastasis. Recently, we have reported that miR-675-5p promotes glioma growth by stabilizing HIF1α; here, by use of the syngeneic cell lines we investigated the role of the miR-675-5p in colon cancer metastasis.Our results show that miR-675-5p, over expressed in metastatic colon cancer cells, participates to tumour progression by regulating HIF1α induced EMT...
January 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/28051134/validation-of-suitable-normalizers-for-mir-expression-patterns-analysis-covering-tumour-heterogeneity
#4
C Morata-Tarifa, M Picon-Ruiz, C Griñan-Lison, H Boulaiz, M Perán, M A Garcia, J A Marchal
Oncogenic microRNAs (miRs) have emerged as diagnostic biomarkers and novel molecular targets for anti-cancer drug therapies. Real-time quantitative PCR (qPCR) is one of the most powerful techniques for analyzing miRs; however, the use of unsuitable normalizers might bias the results. Tumour heterogeneity makes even more difficult the selection of an adequate endogenous normalizer control. Here, we have evaluated five potential referenced small RNAs (U6, rRNA5s, SNORD44, SNORD24 and hsa-miR-24c-3p) using RedFinder algorisms to perform a stability expression analysis in i) normal colon cells, ii) colon and breast cancer cell lines and iii) cancer stem-like cell subpopulations...
January 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28039449/expression-analysis-of-microrna-as-prognostic-biomarkers-in-colorectal-cancer
#5
Jie Yang, Dongling Ma, Andrew Fesler, Haiyan Zhai, Apisri Leamniramit, Wenzhe Li, Song Wu, Jingfang Ju
microRNA (miRNA) based biomarkers have unique advantages due to their critical regulatory function, superior stability, and relatively small number compared to mRNAs. A number of miRNAs play key roles in colon cancer stem cell chemoresistance and have clinical potential as prognostic biomarkers. The purpose of this study is to systematically validate the prognostic potential of miRNAs in colorectal cancer. In this study, we validated the prognostic potential of a panel of miRNAs using 205 stage II, III, and IV colorectal cancer specimens by qRT-PCR analysis...
December 26, 2016: Oncotarget
https://www.readbyqxmd.com/read/28035913/microrna-137-chemosensitizes-colon-cancer-cells-to-the-chemotherapeutic-drug-oxaliplatin-oxa-by-targeting-ybx1
#6
Yunsheng Guo, Yan Pang, Xia Gao, Min Zhao, Xin Zhang, Hao Zhang, Bing Xuan, Yimin Wang
The mechanisms underlying oxaliplatin (OXA) resistance in colon cancer cells are not fully understood. MicroRNAs (miRNAs) play important roles in tumorigenesis and drug resistance. However, the relationship between miRNA and OXA resistance in colon cancer cells has not been previously explored. In this study, we utilized microRNA microarray analysis and real-time PCR to verify that miR-93, miR-191, miR-137, miR-181 and miR-491-3p were significantly down-regulated and that miR-96, miR-21, miR-22, miR-15b and miR-92 were up-regulated in both HCT-15/OXA and SW480/OXA cell lines...
December 23, 2016: Cancer Biomarkers: Section A of Disease Markers
https://www.readbyqxmd.com/read/28035390/influence-of-microrna%C3%A2-34a-on-proliferation-invasion-and-metastasis-of-hct116-cells
#7
Chunyan Li, Shuming Lu, Yingyan Wang, Shibin Guo, Tianyu Zhao, Xiaosheng Wang, Bo Song
The aim of the present study was to investigate the role of microRNA (miR)‑34a expression in the proliferation, invasion and metastasis of colon cancer and its underlying mechanisms. HCT116 cells were cultured in high‑sugar Dulbecco's modified Eagle's medium (DMEM) containing 10% fetal bovine serum and 1000 U/ml penicillin‑streptomycin. Following digestion and resuspension, the cells were used for transfection, expression and western blot analysis. HCT116 cells from miR‑34a transfection, negative control and blank control groups were seeded into a 96‑well plate at a density of 105 cells/ml, and 200 µl complete DMEM was added...
February 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28032929/downregulation-of-mir-100-mir-125b-is-associated-with-lymph-node-metastasis-in-early-colorectal-cancer-with-submucosal-invasion
#8
Yasuteru Fujino, Shunsaku Takeishi, Kensei Nishida, Koichi Okamoto, Naoki Muguruma, Tetsuo Kimura, Shinji Kitamura, Hiroshi Miyamoto, Akiko Fujimoto, Jun Higashijima, Mitsuo Shimada, Kazuhito Rokutan, Tetsuji Takayama
A majority of early colorectal cancers (CRCs) with submucosal invasion undergo surgical operation despite very low incidence of lymph node metastasis. Our study aimed to identify microRNAs (miRNAs) specifically responsible for lymph node metastasis in submucosal CRCs. MicroRNA microarray analysis revealed that miR-100 and miR-125b expression levels were significantly lower in CRC tissues with lymph node metastases than in those without metastases. These results were validated by quantitative real-time PCR in a larger set of clinical samples...
December 29, 2016: Cancer Science
https://www.readbyqxmd.com/read/28032860/osteopontin-facilitates-tumor-metastasis-by-regulating-epithelial-mesenchymal-plasticity
#9
Rongjie Jia, Yingchao Liang, Rui Chen, Guoke Liu, Hao Wang, Min Tang, Xuyu Zhou, Huajing Wang, Yang Yang, Huafeng Wei, Bohua Li, Yipeng Song, Jian Zhao
Tumor metastasis leads to high mortality; therefore, understanding the mechanisms that underlie tumor metastasis is crucial. Generally seen as a secretory protein, osteopontin (OPN) is involved in multifarious pathophysiological events. Here, we present a novel pro-metastatic role of OPN during metastatic colonization. Unlike secretory OPN (sOPN), which triggers the epithelial-mesenchymal transition (EMT) to initiate cancer metastasis, intracellular/nuclear OPN (iOPN) induces the mesenchymal-epithelial transition (MET) to facilitate the formation of metastases...
December 29, 2016: Cell Death & Disease
https://www.readbyqxmd.com/read/28025745/microrna-98-suppress-warburg-effect-by-targeting-hk2-in-colon-cancer-cells
#10
Weimin Zhu, Yijiao Huang, Qi Pan, Pei Xiang, Nanlan Xie, Hao Yu
BACKGROUND: Warburg effect is a hallmark of cancer cells. Accumulating evidence suggests that microRNAs (miRs) could regulate such metabolic reprograming. Aberrant expression of miR-98 has been observed in many types of cancers. However, its functions and significance in colon cancer remain largely elusive. AIMS: To investigate miR-98 expression and the biological functions in colon cancer progression. METHODS: miR-98 expression levels were determined by quantitative RT-PCR in 215 cases of colon cancer samples...
December 26, 2016: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/28017891/self-immolative-nanoparticles-for-simultaneous-delivery-of-microrna-and-targeting-of-polyamine-metabolism-in-combination-cancer-therapy
#11
Ying Xie, Tracy Murray-Stewart, Yazhe Wang, Fei Yu, Jing Li, Laurence J Marton, Robert A Casero, David Oupický
Combination of anticancer drugs with therapeutic microRNA (miRNA) has emerged as a promising anticancer strategy. However, the promise is hampered by a lack of desirable delivery systems. We report on the development of self-immolative nanoparticles capable of simultaneously delivering miR-34a mimic and targeting dysregulated polyamine metabolism in cancer. The nanoparticles were prepared from a biodegradable polycationic prodrug, named DSS-BEN, which was synthesized from a polyamine analog N(1),N(11)-bisethylnorspermine (BENSpm)...
December 23, 2016: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/27991929/mir-19a-promotes-colitis-associated-colorectal-cancer-by-regulating-tumor-necrosis-factor-alpha-induced-protein-3-nf-%C3%AE%C2%BAb-feedback-loops
#12
T Wang, X Xu, Q Xu, J Ren, S Shen, C Fan, Y Hou
Chronic inflammation is believed to have a crucial role in colon cancer development. MicroRNA (miRNA) deregulation is common in human colorectal cancers, but little is known regarding whether miRNA drives tumor progression by regulating inflammation. Here, we showed that miR-19a can promote colitis and colitis-associated colon cancer (CAC) development using a CAC mouse model and an acute colitis mouse model. Tumor necrosis factor-α (TNF-α) stimulation can increase miR-19a expression, and upregulated miR-19a can in turn activate nuclear factor (NF)-κB signaling and TNF-α production by targeting TNF alpha-induced protein 3 (TNFAIP3)...
December 19, 2016: Oncogene
https://www.readbyqxmd.com/read/27991682/the-role-of-metastasis-associated-in-colon-cancer-1-macc1-in-endometrial-carcinoma-tumorigenesis-and-progression
#13
Shuo Chen, Zhi-Hong Zong, Dan-Dan Wu, Kai-Xuan Sun, Bo-Liang Liu, Yang Zhao
Metastasis-associated in colon cancer-1 (MACC1), has recently been identified as a key regulator in the progression of many cancers. However, its role in endometrial carcinoma (EC) remains unknown. MACC1 expression was determined in EC and normal endometrial tissues by immunohistochemistry. EC cell phenotypes and related molecules were examined after MACC1 downregulation by Small interfering RNA (siRNA) or microRNA (miRNA) transfection. We found that MACC1 was highly expressed in EC tissues than normal samples, and was significantly different in FIGO staging (I and II vs...
December 19, 2016: Molecular Carcinogenesis
https://www.readbyqxmd.com/read/27986894/alternative-splicing-of-the-occ-1-gene-generates-three-splice-variants-and-a-novel-exonic-microrna-which-regulate-the-wnt-signaling-pathway
#14
Hadi Najafi, Bahram M Soltani, Sadat Dokanehiifard, Shirzad Nasiri, Seyed Javad Mowla
The Wnt signaling pathway is hyperactivated in most colorectal cancers (CRC). Finding new regulators of this pathway represents the potential for cancer diagnosis or treatment. OCC-1 was initially reported as an up-regulated gene in colon carcinoma, without knowing its mechanism of action. Here, two novel transcript variants and an exonic microRNA that originated from the OCC-1 gene are reported, showing positive effects on Wnt activity. Up-regulation of the known OCC-1 variant (assigned as OCC-1A/B) was limited to CRC, and its overexpression increased survival of CRC-originated SW480 cells (Wnt(+)), while resulting in apoptosis of Wnt-suppressed SW480 cells or HeLa cells (Wnt(-)) detected by PI staining...
January 2017: RNA
https://www.readbyqxmd.com/read/27974852/four-micrornas-signature-for-survival-prognosis-in-colon-cancer-using-tcga-data
#15
Jian Xu, Jian Zhao, Rui Zhang
This study aims to develop microRNA expression signature for colon cancer survival prognosis based on the Cancer Genomic Common database. miRNAs levels between colon cancer and non-cancer tissues were screened by t-test (p < 0.05). Kaplan-Meier survival method was used to discriminate survival significant miRNAs, followed by miRNAs index accumulation to power the miRNAs-survival reliability. In the end, we test the selected miRNAs in HT126 colon cancer cells to validate its anti-cancer effect. The study identified a 84-miRNAs signature...
December 15, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27956498/h3k27-acetylation-activated-long-non-coding-rna-ccat1-affects-cell-proliferation-and-migration-by-regulating-spry4-and-hoxb13-expression-in-esophageal-squamous-cell-carcinoma
#16
Erbao Zhang, Liang Han, Dandan Yin, Xuezhi He, Linzhi Hong, Xinxin Si, Mantang Qiu, Tongpeng Xu, Wei De, Lin Xu, Yongqian Shu, Jinfei Chen
Recently, long non-coding RNAs (lncRNAs) have been shown to have important regulatory roles in human cancer biology. In our study, we found that lncRNA CCAT1, whose expression is significantly increased and is correlated with outcomes in Esophageal Squamous Cell Carcinoma (ESCC). Consecutive experiments confirmed that H3K27-acetylation could activate expression of colon cancer associated transcript-1 (CCAT1). Further experiments revealed that CCAT1 knockdown significantly repressed the proliferation and migration both in vitro and in vivo RNA-seq analysis revealed that CCAT1 knockdown preferentially affected genes that are linked to cell proliferation, cell migration and cell adhesion...
December 11, 2016: Nucleic Acids Research
https://www.readbyqxmd.com/read/27938501/mir-107-promotes-proliferation-and-inhibits-apoptosis-of-colon-cancer-cells-by-targeting-prostate-apoptosis-responde-4-par4
#17
Fen Liu, Shaojun Liu, Feiyan Ai, Decai Zhang, Zhiming Xiao, Xinmin Nie, Yunfeng Fu
Colorectal cancer (CRC) is one of the most common malignancies with high incidence and mortality around the world. However, the pathogenesis of CRC carcinogenesis is still unexplored. In this study, we investigated the role of miR-107 in the regulation of CRC cell proliferation and apoptosis. Firstly, the expression of miR-107 was observed to be aberrantly increased in human CRC tumor tissues and cell lines compared to the control colonic tissues and colon epithelial cells. Further study showed that the proliferation and apoptosis capacity of human CRC SW480 and LoVo cells were aberrantly regulated by miR-107...
29, 2016: Oncology Research
https://www.readbyqxmd.com/read/27930363/upregulated-mir-16-expression-is-an-independent-indicator-of-relapse-and-poor-overall-survival-of-colorectal-adenocarcinoma-patients
#18
Marios A Diamantopoulos, Christos K Kontos, Dimitrios Kerimis, Iordanis N Papadopoulos, Andreas Scorilas
BACKGROUND: Colorectal adenocarcinoma is one of the most common malignant tumors of the gastrointestinal tract and the second leading cause of cancer-related deaths among adults in Western countries. miR-16 is heavily involved in cancer progression. In this study, we examined the potential diagnostic and prognostic utility of miR-16 expression in colorectal adenocarcinoma. METHODS: Total RNA was extracted from 182 colorectal adenocarcinoma specimens and 86 non-cancerous colorectal mucosae...
December 8, 2016: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/27926494/p38-and-jnk-pathways-control-e-selectin-dependent-extravasation-of-colon-cancer-cells-by-modulating-mir-31-transcription
#19
Liang Zhong, Bryan Simoneau, Jacques Huot, Martin J Simard
Extravasation of circulating cancer cells is a key event of metastatic dissemination that is initiated by the adhesion of cancer cells to vascular endothelial cells. It requires the interaction between adhesion receptors such as E-selectin present on endothelial cells and their ligands on cancer cells. Notably, E-selectin influences the metastatic potential of breast, bladder, gastric, pancreatic, and colorectal carcinoma as well as of leukemia and lymphoma. Here, we show that E-selectin expression induced by the pro-inflammatory cytokine IL-1β is directly and negatively regulated by miR-31...
December 2, 2016: Oncotarget
https://www.readbyqxmd.com/read/27910062/plasma-and-saliva-mir-21-expression-in-colorectal-cancer-patients
#20
A A Sazanov, E V Kiselyova, A A Zakharenko, M N Romanov, M I Zaraysky
MicroRNA-21 (miR-21) expression was quantified by real-time qRT-PCR in peripheral blood and saliva samples obtained from patients diagnosed with colorectal cancer (CRC) of varying degrees of malignancy and healthy volunteers. All patients had adenocarcinoma located in the distal colon at different stages. Significant differences were detected between the control group and the total experimental group of CRC patients (plasma, P = 0.0001; saliva, P = 5e-12). MiR-21 expression was also significantly different in certain subgroups of patients with CRC disease stages II-IV as compared to the control group...
December 2, 2016: Journal of Applied Genetics
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