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https://www.readbyqxmd.com/read/28734087/density-dependence-governs-when-population-responses-to-multiple-stressors-are-magnified-or-mitigated
#1
Emma E Hodgson, Timothy E Essington, Benjamin S Halpern
Population endangerment typically arises from multiple, potentially interacting anthropogenic stressors. Extensive research has investigated the consequences of multiple stressors on organisms, frequently focusing on individual life stages. Less is known about population-level consequences of exposure to multiple stressors, especially when exposure varies through life. We provide the first theoretical basis for identifying species at risk of magnified effects from multiple stressors across life history. By applying a population-modeling framework, we reveal conditions under which population responses from stressors applied to distinct life stages are either magnified (synergistic) or mitigated...
July 22, 2017: Ecology
https://www.readbyqxmd.com/read/28732392/systematic-assessment-with-i-scan-magnification-endoscopy-and-acetic-acid-improves-dysplasia-detection-in-patients-with-barrett-s-esophagus
#2
Gideon Lipman, Raf Bisschops, Vinay Sehgal, Jacobo Ortiz-Fernández-Sordo, Rami Sweis, Jose M Esteban, Rifat Hamoudi, Matthew R Banks, Krish Ragunath, Laurence B Lovat, Rehan J Haidry
Background and study aims Enhanced endoscopic imaging with chromoendoscopy may improve dysplasia recognition in patients undergoing assessment of Barrett's esophagus (BE). This may reduce the need for random biopsies to detect more dysplasia. The aim of this study was to assess the effect of magnification endoscopy with I-SCAN (Pentax, Tokyo, Japan) and acetic acid (ACA) on dysplasia detection in BE using a novel mucosal and vascular classification system. Methods BE segments and suspicious lesions were recorded with high definition white-light and magnification endoscopy enhanced using all I-SCAN modes in combination...
July 21, 2017: Endoscopy
https://www.readbyqxmd.com/read/28731851/the-frequency-of-occurrence-of-fish-shaped-red-blood-cells-in-different-haematologic-disorders
#3
Christoph Robier, Carolin Körber, Franz Quehenberger, Manfred Neubauer, Albert Wölfler
BACKGROUND: Red blood cells (RBC) resembling the silhouette of a fish are rarely observed in peripheral blood (PB) smears. In this study, we determined the frequency of occurrence of fish-shaped RBC in different haematologic diseases. METHODS: We examined PB smears of patients with iron deficiency anaemia (IDA) (n=23), β-thalassaemia minor (BTM) (n=30), sickle cell disease (SCD) (n=7), autoimmune haemolytic anaemia (AIHA) (n=13), microangiopathic haemolytic anaemia (MAHA) (n=11), hereditary sphaerocytosis (HS) (n=4), hereditary elliptocytosis (HE) (n=3), vitamin B12 and folate deficiency (n=15), anaemia in liver disease (LD) (n=17), myelodysplastic syndrome (MDS) (n=15), acute myeloid leukaemia (AML) (n=29), chronic myeloid leukaemia (CML) (n=18), primary myelofibrosis (PMF) (n=12), chronic myelo-monocytic leukaemia (CMML) (n=15) and 21 healthy controls by light microscopy for the occurrence of fish-shaped erythrocytes...
July 21, 2017: Clinical Chemistry and Laboratory Medicine: CCLM
https://www.readbyqxmd.com/read/28731057/idiopathic-macular-holes-and-direction-of-vitreomacular-traction-structural-changes-and-surgical-outcomes
#4
C-Y Tsai, Y-T Hsieh, T-T Lai, C-M Yang
PurposeTo compare the structural changes, clinical course, and treatment outcomes of vertical and horizontal vitreomacular traction (VMT) induced impending macular holes (IMHs) and full-thickness macular holes (FTMHs).MethodsIn this retrospective study, 23 and 32 cases of IMHs and FTMHs, respectively, were analyzed. The IMH cases were divided into two subgroups: IMH with and without foveal detachment. Vitreofoveal traction angles (TAs) between the inner retinal surface and posterior hyaloid were measured from horizontal and vertical optical coherence tomography (OCT) images by using the trigonometric function (the angle equals the arctangent of the height over the base) after adjustments for magnification factors...
July 21, 2017: Eye
https://www.readbyqxmd.com/read/28729794/assessment-of-resin-dentin-interfacial-morphology-of-two-ethanol-based-universal-adhesives-a-scanning-electron-microscopy-study
#5
Mohamed Moustafa Awad
OBJECTIVE: The objective of this study was to assess the resin-dentin interfacial morphology created by two universal adhesives using scanning electron microscopy (SEM). MATERIALS AND METHODS: The occlusal surfaces of ten (n = 5) molars were reduced to expose a flat surface of dentin. Two universal adhesives, Scotchbond Universal Adhesive and Tetric N-Bond Universal, were independently applied to air-dried dentin. Light-cured resin-based composite restorative materials were used to incrementally build a composite "buildup...
April 2017: European Journal of Dentistry
https://www.readbyqxmd.com/read/28728043/autofocus-on-moving-object-in-scanning-electron-microscope
#6
Andrey V Kudryavtsev, Sounkalo Dembélé, Nadine Piat
The sharpness of the images coming from a Scanning Electron Microscope (SEM) is a very important property for many computer vision applications at micro- and nanoscale. It represents how much object details are distinctive in the images: the object may be perceived sharp or blurred. Image sharpness highly depends on the value of focal distance, or working distance in the case of the SEM. Autofocus is the technique allowing to automatically adjust the working distance to maximize the sharpness. Most of the existing algorithms allows working only with a static object which is enough for the tasks of visualization, manual microanalysis or microcharacterization...
July 12, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/28727390/use-of-indocyanine-green-angiography-in-microsurgical-subinguinal-varicocelectomy-lessons-learned-from-our-initial-experience
#7
Chak-Lam Cho, Kwan-Lun Ho, Wayne Kwun-Wai Chan, Ringo Wing-Hong Chu, In-Chak Law
Microsurgical subinguinal varicocelectomy (MSV) is generally considered the gold standard nowadays in view of the lower risk of complications and recurrence. To achieve complete ligation of veins while preserving testicular artery (TA) during the procedure remains challenging despite the application of high power optical magnification and micro-Doppler ultrasonography. The use of intraoperative indocyanine green angiography (ICGA) with infrared fluorescence operative micro-scope in MSV potentially lowers the incidence of TA injury and shortens the learning curve of nov-ice surgeons...
July 20, 2017: International Braz J Urol: Official Journal of the Brazilian Society of Urology
https://www.readbyqxmd.com/read/28722051/holographic-microscope-slide-in-a-spatio-temporal-imaging-modality-for-reliable-3d-cell-counting
#8
Biagio Mandracchia, Vittorio Bianco, Zhe Wang, Martina Mugnano, Alessia Bramanti, Melania Paturzo, Pietro Ferraro
In the current trend of miniaturization and simplification of imaging flow cytometry, Lab-on-a-Chip (LoC) microfluidic devices represent an innovative and cost-effective solution. In this framework, we propose for the first time a novel platform based on the compactness of a holographic microscope slide (HMS) in combination with the new computational features of space-time digital holography (STDH) that uses a 1D linear sensor array (LSA) instead of 2D CCD or CMOS cameras to respond to real diagnostic needs...
July 19, 2017: Lab on a Chip
https://www.readbyqxmd.com/read/28720970/effect-of-rice-bran-oil-spread-on-the-physical-sensory-and-fatty-acid-profile-of-cake
#9
Rizwana Shaik, Aparna Kuna, Mohibbe Azam, Ram Tilathoo, Manorama Kanuri, Geetha Samala
Studies were carried out to replace hydrogenated fat (HF) with rice bran oil (RBO) and two varieties of rice bran oil spread RBOS1 and RBOS2 in the preparation of cake. Physico-chemical properties, sensory properties, scanning electronic microscopic (SEM) study and fatty acid estimation with reference to trans fatty acids of cake made with control and experimental samples were studied. The best acceptable cake among the four i.e., RBOS2 cake was selected for consumer evaluation along with control (HF) cake...
June 2017: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/28719002/eyeslam-real-time-simultaneous-localization-and-mapping-of-retinal-vessels-during-intraocular-microsurgery
#10
Daniel Braun, Sungwook Yang, Joseph N Martel, Cameron N Riviere, Brian C Becker
BACKGROUND: Fast and accurate mapping and localization of the retinal vasculature is critical to increasing the effectiveness and clinical utility of robot-assisted intraocular microsurgery such as laser photocoagulation and retinal vessel cannulation. METHODS: The proposed EyeSLAM algorithm delivers 30 Hz real-time simultaneous localization and mapping of the human retina and vasculature during intraocular surgery, combining fast vessel detection with 2D scan-matching techniques to build and localize a probabilistic map of the vasculature...
July 18, 2017: International Journal of Medical Robotics + Computer Assisted Surgery: MRCAS
https://www.readbyqxmd.com/read/28712847/pluri-iq-quantification-of-embryonic-stem-cell-pluripotency-through-an%C3%A2-image-based-analysis-software
#11
Tânia Perestrelo, Weitong Chen, Marcelo Correia, Christopher Le, Sandro Pereira, Ana S Rodrigues, Maria I Sousa, João Ramalho-Santos, Denis Wirtz
Image-based assays, such as alkaline phosphatase staining or immunocytochemistry for pluripotent markers, are common methods used in the stem cell field to assess pluripotency. Although an increased number of image-analysis approaches have been described, there is still a lack of software availability to automatically quantify pluripotency in large images after pluripotency staining. To address this need, we developed a robust and rapid image processing software, Pluri-IQ, which allows the automatic evaluation of pluripotency in large low-magnification images...
July 3, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28700724/optim-optical-projection-tomography-integrated-microscope-using-open-source-hardware-and-software
#12
Thomas Watson, Natalie Andrews, Samuel Davis, Laurence Bugeon, Margaret D Dallman, James McGinty
We describe the implementation of an OPT plate to perform optical projection tomography (OPT) on a commercial wide-field inverted microscope, using our open-source hardware and software. The OPT plate includes a tilt adjustment for alignment and a stepper motor for sample rotation as required by standard projection tomography. Depending on magnification requirements, three methods of performing OPT are detailed using this adaptor plate: a conventional direct OPT method requiring only the addition of a limiting aperture behind the objective lens; an external optical-relay method allowing conventional OPT to be performed at magnifications >4x; a remote focal scanning and region-of-interest method for improved spatial resolution OPT (up to ~1...
2017: PloS One
https://www.readbyqxmd.com/read/28694064/rna-fluorescence-in-situ-hybridization-for-high-content-screening
#13
Emmanuelle Querido, Lynda Dekakra-Bellili, Pascal Chartrand
Single molecule RNA imaging using fluorescent in situ hybridization (FISH) can provide quantitative information on mRNA abundance and localization in a single cell. There is now a growing interest in screening for modifiers of RNA abundance and/or localization. For instance, microsatellite expansion within RNA can lead to toxic gain-of-function via mislocalization of these transcripts into RNA aggregate and sequestration of RNA-binding proteins. Screening for inhibitors of these RNA aggregate can be performed by high-throughput RNA FISH...
July 7, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28692403/accurate-region-of-interest-recovery-improves-the-measurement-of-the-cell-migration-rate-in-the-in-vitro-wound-healing-assay
#14
Cesar Bedoya, Andrés Cardona, July Galeano, Fabián Cortés-Mancera, Patrick Sandoz, Artur Zarzycki
The wound healing assay is widely used for the quantitative analysis of highly regulated cellular events. In this essay, a wound is voluntarily produced on a confluent cell monolayer, and then the rate of wound reduction (WR) is characterized by processing images of the same regions of interest (ROIs) recorded at different time intervals. In this method, sharp-image ROI recovery is indispensable to compensate for displacements of the cell cultures due either to the exploration of multiple sites of the same culture or to transfers from the microscope stage to a cell incubator...
July 1, 2017: SLAS Technology
https://www.readbyqxmd.com/read/28691449/evaluation-of-the-intraepithelial-papillary-capillary-loops-in-benign-and-malignant-oral-lesions-by-in-vivo-virtual-chromoendoscopic-magnification-a-preliminary-study
#15
M Contaldo, A Lucchese, E Gentile, C Zulli, M Petruzzi, D Lauritano, M R Amato, P Esposito, G Riegler, R Serpico
This preliminary study aims to establish the Virtual Chromoendoscopic Magnification (VCM) feasibility to visualize and distinguish the Intraepithelial Papillary Capillary Loops (IPCL) patterns of benign oral pathologies from malignant ones. Thirty-one consecutive subjects affected by oral lesions/pathologies underwent white light examination and VCM imaging by the Narrow Band Imaging System (Olympus Medical Systems Corp., Tokyo, Japan). A class system of four IPCL types corresponding to progressive vessel disarray was adopted...
April 2017: Journal of Biological Regulators and Homeostatic Agents
https://www.readbyqxmd.com/read/28689366/micro-ct-and-fe-sem-enamel-analyses-of-calcium-based-agent-application-after-bleaching
#16
Mauricio Neves Gomes, Flávia Pires Rodrigues, Nick Silikas, Carlos Eduardo Francci
OBJECTIVES: The objective of the present study is to evaluate the effects of casein phosphopeptide-amorphous calcium phosphate (CPP-ACP) on bleached enamel. MATERIALS AND METHODS: A bleaching agent (35% hydrogen peroxide) was applied, 4 × 8 min on premolar teeth (n = 8). A CPP-ACP paste was applied for 7 days. Prior and post-treatment, microtomography images were obtained and 3D regions of interest (ROIs) were selected, from outer enamel, extending to 110...
July 8, 2017: Clinical Oral Investigations
https://www.readbyqxmd.com/read/28689081/high-throughput-semi-automated-quantitative-stem-mass-measurement-of-supported-metal-nanoparticles-using-a-conventional-tem-stem
#17
Stephen D House, Yuxiang Chen, Rongchao Jin, Judith C Yang
The adaptation of quantitative STEM techniques to enable atom-counting in supported metal nanoparticles with a modern, conventional (non-aberration-corrected) TEM/STEM (a JEOL JEM2100F) without the need for any modifications or special hardware is presented. No image simulation is required, either. This technique enables the practical analysis of the size, mass, and basic shape information of statistically robust populations of hundreds to thousands of nanoparticles. The methods for performing the necessary calibrations of the microscope and images are detailed...
July 3, 2017: Ultramicroscopy
https://www.readbyqxmd.com/read/28687293/antero-posterior-ap-pelvis-x-ray-imaging-on-a-trolley-impact-of-trolley-design-mattress-design-and-radiographer-practice-on-image-quality-and-radiation-dose
#18
J R Tugwell, A England, P Hogg
INTRODUCTION: Physical and technical differences exist between imaging on an x-ray tabletop and imaging on a trolley. This study evaluates how trolley imaging impacts image quality and radiation dose for an antero-posterior (AP) pelvis projection whilst subsequently exploring means of optimising this imaging examination. METHODS: An anthropomorphic pelvis phantom was imaged on a commercially available trolley under various conditions. Variables explored included two mattresses, two image receptor holder positions, three source to image distances (SIDs) and four mAs values...
August 2017: Radiography
https://www.readbyqxmd.com/read/28686750/magnification-for-the-dermatologic-surgeon
#19
Hubert M Chodkiewicz, Aaron K Joseph
Ergonomic practice increases the productivity, quality, and longevity of the dermatologic surgeon. When used properly, magnification devices can be ergonomic and beneficial additions to the dermatologic surgeon's practice. Herein, we review the available magnification options for the dermatologic surgeon and evaluate the options based on cost, design, and functional advantages and disadvantages. Magnification for the dermatologic surgeon may be a useful tool for a healthier, more efficient, and higher-quality practice...
June 2017: Cutis; Cutaneous Medicine for the Practitioner
https://www.readbyqxmd.com/read/28685858/diagnostic-performance-of-routine-esophagogastroduodenoscopy-using-magnifying-endoscope-with-narrow-band-imaging-for-gastric-cancer
#20
Shoichi Yoshimizu, Yorimasa Yamamoto, Yusuke Horiuchi, Masami Omae, Toshiyuki Yoshio, Akiyoshi Ishiyama, Toshiaki Hirasawa, Tomohiro Tsuchida, Junko Fujisaki
OBJECTIVES: In Japan, an increase in the number of routine esophagogastroduodenoscopy procedures is expected because several studies have reported that endoscopy screenings have reduced gastric cancer mortality. Magnifying narrow-band imaging has been reported to be effective for accurate diagnosis of gastric abnormalities such as cancers, adenomas, and intestinal metaplasia. However, the efficacy of this method in routine esophagogastroduodenoscopy has not been clarified. METHODS: We divided 3,763 patients into two groups...
July 7, 2017: Digestive Endoscopy: Official Journal of the Japan Gastroenterological Endoscopy Society
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