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peptide self-assembly

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https://www.readbyqxmd.com/read/29341600/combination-of-heparin-binding-peptide-and-heparin-cell-surface-coatings-for-mesenchymal-stem-cell-spheroid-assembly
#1
Jennifer Lei, William L Murphy, Johnna S Temenoff
Microtissues containing multiple cell types have been used in both in vitro models and in vivo tissue repair applications. However, to improve through-put, there is a need to develop a platform that supports self-assembly of a large number of 3D microtissues containing multiple cell types in a dynamic suspension system. Thus, the objective of this study was to exploit the binding interaction between the negatively charged glycosaminoglycan, heparin, and a known heparin binding peptide to establish a method that promotes assembly of mesenchymal stem cell (MSC) spheroids into larger aggregates...
January 17, 2018: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29340383/alamethicin-self-assembling-in-lipid-membranes-concentration-dependence-from-pulsed-epr-of-spin-labels
#2
Victoria N Syryamina, Marta De Zotti, Claudio Toniolo, Fernando Formaggio, Sergei A Dzuba
The antimicrobial action of the peptide antibiotic alamethicin (Alm) is commonly related to peptide self-assembling resulting in the formation of voltage-dependent channels in bacterial membranes, which induces ion permeation. To obtain a deeper insight into the mechanism of channel formation, it is useful to know the dependence of self-assembling on peptide concentration. With this aim, we studied Alm F50/5 spin-labeled analogs in a model 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membrane, for peptide-to-lipid (P/L) ratios varying between 1/1500 and 1/100...
January 17, 2018: Physical Chemistry Chemical Physics: PCCP
https://www.readbyqxmd.com/read/29338205/control-of-amphiphile-self-assembly-via-bioinspired-metal-ion-coordination
#3
Abigail S Knight, Josefin Larsson, Jing M Ren, Raghida Bou Zerdan, Shay Seguin, Remy Vrahas, Jianfang Liu, Gang Ren, Craig J Hawker
Inspired by marine siderophores that exhibit a morphological shift upon metal coordination, hybrid peptide-polymer conjugates that assemble into different morphologies based on the nature of the metal ion coordination have been designed. Coupling of a peptide chelator, hexahistidine, with hydrophobic oligostyrene allows a modular strategy to be established for the efficient synthesis and purification of these tunable amphiphiles (oSt(His)6). Remarkably, in the presence of different divalent transition metal ions (Mn, Co, Ni, Cu, Zn, and Cd) a variety of morphologies were observed...
January 17, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29336438/zein-derived-peptides-as-nanocarriers-to-increase-the-water-solubility-and-stability-of-lutein
#4
Yan Jiao, Xiqun Zheng, Ying Chang, Dajing Li, Xiaohong Sun, Xiaolan Liu
Zein and its derived peptides have been used as nanocarriers for bioactive components. Lutein, as well as other xanthophylls, are characterized by blue light filtering and anti-oxidant properties. However, lutein is unstable and has low water solubility, poor absorption, and low bioavailability. In order to protect lutein from oxidative degradation, and to enhance its solubility and dispersibility, stability and bioactivity, lutein-loaded zein nanoparticles (LLZ-NP) and zein-derived peptide nanoparticles (LLZ-PEP-NP) were prepared by the solvent diffusion method...
January 16, 2018: Food & Function
https://www.readbyqxmd.com/read/29334808/identification-of-a-novel-site-of-interaction-between-ataxin-3-and-the-amyloid-aggregation-inhibitor-polyglutamine-binding-peptide-1
#5
Patrick D Knight, Theodoros K Karamanos, Sheena E Radford, Alison E Ashcroft
Amyloid diseases represent a growing social and economic burden in the developed world. Understanding the assembly pathway and the inhibition of amyloid formation is key to developing therapies to treat these diseases. The neurodegenerative condition Machado-Joseph disease is characterised by the self-aggregation of the protein ataxin-3. Ataxin-3 consists of a globular N-terminal Josephin domain, which can aggregate into curvilinear protofibrils, and an unstructured, dynamically disordered C-terminal domain containing three ubiquitin interacting motifs separated by a polyglutamine stretch...
February 2018: European Journal of Mass Spectrometry
https://www.readbyqxmd.com/read/29334733/surface-functionalization-with-carboxylic-acids-by-photochemical-microcontact-printing-and-tetrazole-chemistry
#6
Christoph Buten, Sebastian Lamping, Martin Körsgen, Heinrich F Arlinghaus, Craig Jamieson, Bart Jan Ravoo
In this paper we show that carboxylic acid functionalized molecules can be patterned by photochemical microcontact printing on tetrazole-terminated self-assembled monolayers. Upon irradiation, tetrazoles eliminate nitrogen to form highly reactive nitrile imines, which can be ligated with several different nucleophiles, carboxylic acids being most reactive. As a proof of concept, we immobilized trifluoroacetic acid to monitor the reaction with X-ray photoelectron spectroscopy (XPS). Moreover, we also immobilized peptides and fabricated carbohydrate-lectin as well as biotin-streptavidin microarrays using this method...
January 16, 2018: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29328651/enzymatic-cleavage-of-branched-peptides-for-targeting-mitochondria
#7
Hongjian He, Jiaqing Wang, Huaimin Wang, Ning Zhou, Dongsik Yang, Douglas R Green, Bing Xu
Most of the reported mitochondria targeting molecules are lipophilic and cationic, which may become cytotoxic with accumulation. Here we show enzymatic cleavage of branched peptides that carry negative charges for targeting mitochondria. Conjugating a well-established protein tag (i.e., FLAG-tag) to self-assembling motifs affords the pre-cursors that form micelles. Enzymatic cleavage of the hydrophilic FLAG motif (DDDDK) by enterokinase (ENTK) turns the micelles to nanofibers. After being taken up by cells, the micelles, upon the action of intracellular ENTK, turns into nanofibers to locate mainly at mitochondria...
January 12, 2018: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/29327429/in-vivo-migration-of-endogenous-brain-progenitor-cells-guided-by-an-injectable-peptide-amphiphile-biomaterial
#8
Reza Motalleb, Eric J Berns, Piyush Patel, Julie Gold, Samuel I Stupp, H Georg Kuhn
Biomaterials hold great promise in helping the adult brain regenerate and rebuild after trauma. Peptide amphiphiles (PA) are highly versatile biomaterials, gelling and forming macromolecular structures when exposed to physiological levels of electrolytes. We are here reporting on the first ever in vivo use of self-assembling peptide amphiphile carrying a Tenascin-C signal (E2 Ten-C PA) for the re-direction of endogenous neuroblasts in the rodent brain. The PA forms highly aligned nanofibers, displaying the migratory sequence of Tenascin-C glycoprotein as epitope...
January 12, 2018: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/29325204/a-transformable-chimeric-peptide-for-cell-encapsulation-to-overcome-multidrug-resistance
#9
Chi Zhang, Li-Han Liu, Wen-Xiu Qiu, Yao-Hui Zhang, Wen Song, Lu Zhang, Shi-Bo Wang, Xian-Zheng Zhang
Multidrug resistance (MDR) remains one of the biggest obstacles in chemotherapy of tumor mainly due to P-glycoprotein (P-gp)-mediated drug efflux. Here, a transformable chimeric peptide is designed to target and self-assemble on cell membrane for encapsulating cells and overcoming tumor MDR. This chimeric peptide (C16 -K(TPE)-GGGH-GFLGK-PEG8 , denoted as CTGP) with cathepsin B-responsive and cell membrane-targeting abilities can self-assemble into nanomicelles and further encapsulate the therapeutic agent doxorubicin (termed as CTGP@DOX)...
January 11, 2018: Small
https://www.readbyqxmd.com/read/29316785/intracellular-peptide-self-assembly-a-biomimetic-approach-for-in-situ-nano-drug-preparation
#10
Wei Du, Xiaomu Hu, Weichen Wei, Gaolin Liang
Most of the nano-drugs are pre-prepared by encapsulating or loading the drugs with nano-carriers (e.g., dendrimers, liposomes, micelles, and polymeric nanoparticles). However, besides the low bioavailability and fast excretion of the nano-drugs in vivo, nano-carriers often exhibit in vitro and in vivo cytotoxicity, oxidative stress, and inflammation. Self-assembly is a ubiquitous process in biology where it plays important roles and underlies the formation of a wide variety of complex biological structures...
January 9, 2018: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/29315863/activatable-protein-nanoparticles-for-targeted-delivery-of-therapeutic-peptides
#11
Xi Yu, Xingchun Gou, Peng Wu, Liang Han, Daofeng Tian, Fengyi Du, Zeming Chen, Fuyao Liu, Gang Deng, Ann T Chen, Chao Ma, Jun Liu, Sara M Hashmi, Xing Guo, Xiaolong Wang, Haitian Zhao, Xinran Liu, Xudong Zhu, Kevin Sheth, Qianxue Chen, Louzhen Fan, Jiangbing Zhou
Clinical translation of therapeutic peptides, particularly those that require penetration of the cell membrane or are cytolytic, is a major challenge. A novel approach based on a complementary mechanism, which has been widely used for guided synthesis of DNA or RNA nanoparticles, for de novo design of activatable protein nanoparticles (APNPs) for targeted delivery of therapeutic peptides is described. APNPs are formed through self-assembly of three independent polypeptides based on pairwise coiled-coil dimerization...
January 8, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29306761/peptide-nanoparticles-pnps-modified-disposable-platform-for-sensitive-electrochemical-cytosensing-of-dld-1-cancer-cells
#12
Yesim Tugce Yaman, Öznur Akbal, Gulcin Bolat, Betul Bozdogan, Emir Baki Denkbas, Serdar Abaci
A novel diphenylalaninamid (FFA) based peptide nanoparticles (PNPs) modified pencil graphite electrodes (PGEs) for construction of electrochemical cytosensor was demonstrated for the first time in this study. Scanning electron microscopy (SEM) and transmission electron microscopy (TEM) images revealed the spherical nanostructure of the synthesized FFA based PNPs while attenuated total reflectance-fourier transform infrared (ATR-FTIR) spectra provided information about the structure and conformation of proteins in their structure...
December 25, 2017: Biosensors & Bioelectronics
https://www.readbyqxmd.com/read/29304403/targeting-death-receptors-for-drug-resistant-cancer-therapy-codelivery-of-ptrail-and-monensin-using-dual-targeting-and-stimuli-responsive-self-assembling-nanocomposites
#13
Fan Xu, Huihai Zhong, Ya Chang, Dongdong Li, Hongyue Jin, Meng Zhang, Huiyuan Wang, Chen Jiang, Youqing Shen, Yongzhuo Huang
Chemoresistance remains a formidable hurdle against cancer therapy. Seeking for novel therapy strategies is an urgent need for those who no longer benefit from chemotherapy. Chemoresistance is usually associated with the dysfunction of intrinsic apoptosis. Targeting extrinsic apoptosis via TRAIL signaling and the death receptors could be a potential solution to treat chemoresistant cancer. A highly biocompatible nano system for codelivery of the TRAIL DNA and the death receptor sensitizer monensin was developed, in which low-molecular-weight PEI (LMW-PEI) was crosslinked by the sulfhydryl cyclodextrin via disulfide bonds, and then bound with DNA, thus forming the bioreducible polyplex cores...
December 22, 2017: Biomaterials
https://www.readbyqxmd.com/read/29304115/effects-of-a-self-assembling-peptide-as-a-scaffold-on-bone-formation-in-a-defect
#14
Kei Ando, Shiro Imagama, Kazuyoshi Kobayashi, Kenyu Ito, Mikito Tsushima, Masayoshi Morozumi, Satoshi Tanaka, Masaaki Machino, Kyotaro Ota, Koji Nishida, Yoshihiro Nishida, Naoki Ishiguro
Spinal fusion and bone defect after injuries, removal of bone tumors, and infections need to be repaired by implantation. In an aging society, recovery from these procedures is often difficult. In this study, we found that injection of SPG-178 leads to expression of several bone marker genes and mineralization in vitro, and revealed a significantly higher degree of newly formed bone matrix with use of SPG-178 in vivo. MC3T3-E1 cells were used to evaluate osteoblast differentiation promoted by SPG-178. To analyze gene expression, total RNA was isolated from MC3T3-E1 cells cultured for 7 and 14 days with control medium or SPG-178 medium...
2018: PloS One
https://www.readbyqxmd.com/read/29303206/self-assembly-pathways-and-polymorphism-in-peptide-based-nanostructures
#15
Nikola A Dudukovic, Benjamin C Hudson, Anant K Paravastu, Charles F Zukoski
Dipeptide derivative molecules can self-assemble into space-filling nanofiber networks at low volume fractions (<1%), allowing the formation of molecular gels with tunable mechanical properties. The self-assembly of dipeptide-based molecules is reminiscent of pathological amyloid fibril formation by naturally occurring polypeptides. Fluorenylmethoxycarbonyl-diphenylalanine (Fmoc-FF) is the most widely studied such molecule, but the thermodynamic and kinetic phenomena giving rise to Fmoc-FF gel formation remain poorly understood...
January 5, 2018: Nanoscale
https://www.readbyqxmd.com/read/29302635/supramolecular-peptide-nanofibers-engage-mechanisms-of-autophagy-in-antigen-presenting-cells
#16
Jai S Rudra, Arshad Khan, Tara M Clover, Janice J Endsley, Andrew Zloza, Jin Wang, Chinnaswamy Jagannath
Supramolecular peptide nanofibers are attractive for applications in vaccine development due to their ability to induce strong immune responses without added adjuvants or associated inflammation. Here, we report that self-assembling peptide nanofibers bearing CD4+ or CD8+ T cell epitopes are processed through mechanisms of autophagy in antigen-presenting cells (APCs). Using standard in vitro antigen presentation assays, we confirmed loss and gain of the adjuvant function using pharmacological modulators of autophagy and APCs deficient in multiple autophagy proteins...
December 31, 2017: ACS Omega
https://www.readbyqxmd.com/read/29289731/fabrication-of-two-dimensional-2d-ordered-microsphere-aligned-by-supramolecular-self-assembly-of-formyl-azobenzene-and-dipeptide
#17
Hongchao Ma, Shuo Li, Yanhui Wei, Lei Jiang, Junbai Li
An azobenzene with a terminal formyl group, named as (4-[(3-Formyl-4-hydroxy)phenylazo]benzene (FHPAB)), was synthesized and used to manipulate the self-assembly of diphenylalanine (FF) molecules. Two-dimensional (2D) thin slices which are composed of ordered microspheres have been constructed through supramolecular self-assembly of FF and FHPAB. The FTIR and XPS results indicate that CN covalent bond between FF and FHPAB was generated. Hydrogen bonding and strong π-π interaction between the planar FF-HFPAB conjugates are the driving force to form the FF-HFPAB 2D thin slices...
December 22, 2017: Journal of Colloid and Interface Science
https://www.readbyqxmd.com/read/29286384/synthesis-of-monocyte-targeting-peptide-amphiphile-micelles-for-imaging-of-atherosclerosis
#18
Christopher Poon, Manjima Sarkar, Eun Ji Chung
Atherosclerosis is a major contributor to cardiovascular disease, the leading cause of death worldwide, which claims 17.3 million lives annually. Atherosclerosis is also the leading cause of sudden death and myocardial infarction, instigated by unstable plaques that rupture and occlude the blood vessel without warning. Current imaging modalities cannot differentiate between stable and unstable plaques that rupture. Peptide amphiphiles micelles (PAMs) can overcome this drawback as they can be modified with a variety of targeting moieties that bind specifically to diseased tissue...
November 17, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29284122/application-of-elastin-based-nanoparticles-displaying-antibody-binding-domains-for-a-homogeneous-immunoassay
#19
Tsutomu Sugihara, Masayasu Mie, Eiry Kobatake
Nanoparticles are small size-controlled particles from 1 to 100 nm diameters and characterized by their structure, base material and functional units displayed on their surfaces. In this study, protein-based nanoparticles composed of a hydrophobic elastin-like peptide unit, a hydrophilic aspartic acid-rich peptide unit and displaying antibody binding domains on their surfaces, were designed and genetically synthesized. The constituent fusion proteins, termed ELP-D-C, were found to exist in monomeric form (ELP-D-C/monomer) at low temperature...
December 25, 2017: Analytical Biochemistry
https://www.readbyqxmd.com/read/29284013/creating-a-stem-cell-niche-in-the-inner-ear-using-self-assembling-peptide-amphiphiles
#20
Akihiro J Matsuoka, Zafar A Sayed, Nicholas Stephanopoulos, Eric J Berns, Anil R Wadhwani, Zachery D Morrissey, Duncan M Chadly, Shun Kobayashi, Alexandra N Edelbrock, Tomoji Mashimo, Charles A Miller, Tammy L McGuire, Samuel I Stupp, John A Kessler
The use of human embryonic stem cells (hESCs) for regeneration of the spiral ganglion will require techniques for promoting otic neuronal progenitor (ONP) differentiation, anchoring of cells to anatomically appropriate and specific niches, and long-term cell survival after transplantation. In this study, we used self-assembling peptide amphiphile (PA) molecules that display an IKVAV epitope (IKVAV-PA) to create a niche for hESC-derived ONPs that supported neuronal differentiation and survival both in vitro and in vivo after transplantation into rodent inner ears...
2017: PloS One
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