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peptide self-assembly

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https://www.readbyqxmd.com/read/28745772/molecular-dynamics-simulations-reveal-disruptive-self-assembly-in-dynamic-peptide-libraries
#1
I R Sasselli, I P Moreira, R V Ulijn, T Tuttle
There is significant interest in the use of unmodified self-assembling peptides as building blocks for functional, supramolecular biomaterials. Recently, dynamic peptide libraries (DPLs) have been proposed to select self-assembling materials from dynamically exchanging mixtures of dipeptide inputs in the presence of a nonspecific protease enzyme, where peptide sequences are selected and amplified based on their self-assembling tendencies. It was shown that the results of the DPL of mixed sequences (e.g. starting from a mixture of dileucine, L2, and diphenylalanine, F2) did not give the same outcome as the separate L2 and F2 libraries (which give rise to the formation of F6 and L6), implying that interactions between these sequences could disrupt the self-assembly...
July 26, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28742327/amphiphilic-peptide-nanorods-based-on-oligo-phenylalanine-as-a-biocompatible-drug-carrier
#2
Su Jeong Song, Seulgi Lee, Kyoung-Seok Ryu, Joon Sig Choi
Peptide nanostructure has been widely explored for drug delivery systems in recent studies. Peptides possess comparatively lower cytotoxicity and are more efficient than polymeric carriers. Here, we propose a peptide nanorod system, composed of an amphiphilic oligo-peptide RH3F8 (Arg-His3-Phe8) as a drug delivery carrier. Arginine is an essential amino acid in typical cell penetration peptides, and histidine induces endo/lysosomal escape because of its proton sponge effect. Phenylalanine is introduced to provide rich hydrophobicity for stable self-assembly and drug encapsulation...
July 25, 2017: Bioconjugate Chemistry
https://www.readbyqxmd.com/read/28741923/codelivery-of-trichosanthin-and-albendazole-by-nano-self-assembly-for-overcoming-tumor-multidrug-resistance-and-metastasis
#3
Yisi Tang, Jianming Liang, Aihua Wu, Yingzhi Chen, Pengfei Zhao, Tingting Lin, Meng Zhang, Qin Xu, Jianxin Wang, Yongzhuo Huang
Multidrug resistance (MDR) and metastasis are the major obstacles in cancer chemotherapy. Nanotechnology-based combination therapy is a useful strategy. Recently, the combination of biologics and small drugs has attracted much attention in cancer therapy. Yet, the treatment outcomes are often compromised by the different pharmacokinetics profiles of the co-administered drugs, thus leading to inconsistent drug uptake and suboptimal drug combination at the tumor sites. Nanotechnology-based codelivery offers a promising method to address this problem, which is well demonstrated in the use of small drugs combination...
July 25, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28735028/protein-only-antimicrobial-peptide-containing-recombinant-nanoparticles-with-inherent-built-in-antibacterial-activity
#4
Naroa Serna, Laura Sánchez-García, Alejandro Sánchez-Chardi, Ugutz Unzueta, Mónica Roldán, Ramón Mangues, Esther Vázquez, Antonio Villaverde
The emergence of bacterial antibiotic resistances is a serious concern in human and animal health. In this context, naturally occurring cationic antimicrobial peptides (AMPs) might play a main role in a next generation of drugs against bacterial infections. Taking an innovative approach to design self-organizing functional proteins, we have generated here protein-only nanoparticles with intrinsic AMP microbicide activity. Using a recombinant version of the GWH1 antimicrobial peptide as building block, these materials show a wide antibacterial activity spectrum in absence of detectable toxicity on mammalian cells...
July 19, 2017: Acta Biomaterialia
https://www.readbyqxmd.com/read/28726727/in-vitro-quantified-determination-of-%C3%AE-amyloid-42-peptides-a-biomarker-of-neuro-degenerative-disorders-in-pbs-and-human-serum-using-a-simple-cost-effective-thin-gold-film-biosensor
#5
Yifan Dai, Alireza Molazemhosseini, Chung Chiun Liu
A simple in vitro biosensor for the detection of β-amyloid 42 in phosphate-buffered saline (PBS) and undiluted human serum was fabricated and tested based on our platform sensor technology. The bio-recognition mechanism of this biosensor was based on the effect of the interaction between antibody and antigen of β-amyloid 42 to the redox couple probe of K₄Fe(CN)₆ and K₃Fe(CN)₆. Differential pulse voltammetry (DPV) served as the transduction mechanism measuring the current output derived from the redox coupling reaction...
July 20, 2017: Biosensors
https://www.readbyqxmd.com/read/28723181/mixed-self-assembled-aptamer-and-newly-designed-zwitterionic-peptide-as-antifouling-biosensing-interface-for-electrochemical-detection-of-alpha-fetoprotein
#6
Min Cui, Yu Wang, Mingxia Jiao, Silambarasan Jayachandran, Yumin Wu, Xiaojian Fan, Xiliang Luo
A sensitive and low-fouling aptasensor for alpha-fetoprotein (AFP) was developed based on mixed self-assembled aptamers and newly designed zwitterionic peptides, where densely immobilized peptides formed an antifouling layer to resist nonspecific protein adsorption, and sparsely attached aptamers acted as the recognizing layer to achieve target binding. The obtained biosensing interface responded to the target AFP with a strikingly selective and sensitive manner, exhibited excellent protein-resistant performance even in complex human serum solution, and showed promising feasibility for the quantitative analysis of AFP in real human serum samples...
April 28, 2017: ACS Sensors
https://www.readbyqxmd.com/read/28723067/structural-and-spectroscopic-properties-of-assemblies-of-self-replicating-peptide-macrocycles
#7
Pim W J M Frederix, Julien Idé, Yigit Altay, Gaël Schaeffer, Mathieu Surin, David Beljonne, Anna S Bondarenko, Thomas L C Jansen, Sijbren Otto, Siewert J Marrink
Self-replication at the molecular level is often seen as essential to the early origins of life. Recently a mechanism of self-replication has been discovered in which replicator self-assembly drives the process. We have studied one of the examples of such self-assembling self-replicating molecules to a high level of structural detail using a combination of computational and spectroscopic techniques. Molecular Dynamics simulations of self-assembled stacks of peptide-derived replicators provide insights into the structural characteristics of the system and serve as the basis for semiempirical calculations of the UV-vis, circular dichroism (CD) and infrared (IR) absorption spectra that reflect the chiral organization and peptide secondary structure of the stacks...
July 21, 2017: ACS Nano
https://www.readbyqxmd.com/read/28722055/the-amyloid-architecture-provides-a-scaffold-for-enzyme-like-catalysts
#8
Z S Al-Garawi, B A McIntosh, D Neill-Hall, A A Hatimy, S M Sweet, M C Bagley, L C Serpell
Natural biological enzymes possess catalytic sites that are generally surrounded by a large three-dimensional scaffold. However, the proportion of the protein molecule that participates in the catalytic reaction is relatively small. The generation of artificial or miniature enzymes has long been a focus of research because enzyme mimetics can be produced with high activity at low cost. These enzymes aim to mimic the active sites without the additional architecture contributed by the protein chain. Previous work has shown that amyloidogenic peptides are able to self-assemble to create an active site that is capable of binding zinc and catalysing an esterase reaction...
July 19, 2017: Nanoscale
https://www.readbyqxmd.com/read/28721731/self-assembled-injectable-peptide-hydrogels-capable-of-triggering-antitumor-immune-response
#9
Ruirui Xing, Shukun Li, Ning Zhang, Guizhi Shen, Helmuth Möhwald, Xuehai Yan
Self-assembled peptide hydrogels are particularly appealing for drug delivery, tissue engineering, and anti-tumor therapy due to various advantageous features including excellent biocompatibility and biodegradability, defined molecular and higher organized structures and easy availability. However, the poor mechanical and rheological properties of assembled peptide hydrogels cause difficulties in injection, thus limiting further applications. Herein, injectable peptide-based hydrogels with tunable mechanical and rheological properties were obtained by combination with a positively charged poly-peptide (poly-L-lysine, PLL)...
July 19, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28721045/synthesis-construction-and-evaluation-of-self-assembled-nano-bacitracin-a-as-an-efficient-antibacterial-agent-in-vitro-and-in-vivo
#10
Wei Hong, Xiang Gao, Peng Qiu, Jie Yang, Mingxi Qiao, Hong Shi, Dexian Zhang, Chunlian Tian, Shengli Niu, Mingchun Liu
Bacitracin A (BA) is an excellent polypeptide antibiotic that is active against gram-positive bacteria without triggering multidrug resistance. However, BA is inactive against gram-negative bacteria because of its inability to cross the outer membrane of these cells, and it has strong nephrotoxicity, thus limiting its clinical applications. Nanoantibiotics can effectively localize antibiotics to the periplasmic space of bacteria while decreasing the adverse effects of antibiotics. In this study, biodegradable hydrophobic copolymers of poly (d,l-lactide-co-glycolide) (PLGA) were attached to the N-termini of BA to design a novel class of self-assembled nano-bacitracin A (nano-BAs), and their potential as antibacterial agents was evaluated in vitro and in vivo...
2017: International Journal of Nanomedicine
https://www.readbyqxmd.com/read/28720097/a-versatile-papaya-mosaic-virus-papmv-vaccine-platform-based-on-sortase-mediated-antigen-coupling
#11
Ariane Thérien, Mikaël Bédard, Damien Carignan, Gervais Rioux, Louis Gauthier-Landry, Marie-Ève Laliberté-Gagné, Marilène Bolduc, Pierre Savard, Denis Leclerc
BACKGROUND: Flexuous rod-shaped nanoparticles made of the coat protein (CP) of papaya mosaic virus (PapMV) have been shown to trigger innate immunity through engagement of toll-like receptor 7 (TLR7). PapMV nanoparticles can also serve as a vaccine platform as they can increase the immune response to fused peptide antigens. Although this approach shows great potential, fusion of antigens directly to the CP open reading frame (ORF) is challenging because the fused peptides can alter the structure of the CP and its capacity to self assemble into nanoparticles-a property essential for triggering an efficient immune response to the peptide...
July 18, 2017: Journal of Nanobiotechnology
https://www.readbyqxmd.com/read/28719744/high-resolution-insights-into-the-stepwise-self-assembly-of-nanofiber-from-bioactive-peptides
#12
Fude Sun, Long Chen, Xiufang Ding, Lida Xu, Xirui Zhou, Peng Wei, Jun-Feng Liang, Shi-Zhong Luo
Peptide self-assembly has a profound biological significance since self-assembled bioactive peptides are gifted with improved bioactivity as well as life-span. In this study, peptide self-assembly was investigated using a therapeutic peptide, PTP-7S (EENFLGALFKALSKLL). Combining experiments of atomic force microscopy (AFM), circular dichroism (CD) and 8-anilino-1-naphthalenesulfonic acid (ANS) fluorescence spectra, PTP-7S showing α-helix was found self-assembling into nanofibers in solution. Relying on the coarse-grained (CG) dynamic simulations, the self-assembling of PTP-7S was revealed as a stepwise process that peptide monomers first clustered into peptide-assembling units (PUs) with charged surface, and then the PUs integrated together to construct nano-fibril aggregates...
July 18, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28719579/using-primate-neural-stem-cells-cultured-in-self-assembling-peptide-nanofiber-scaffolds-to-repair-injured-spinal-cords-in-rats
#13
J-C Ye, Y Qin, Y-F Wu, P Wang, Y Tang, L Huang, M-J Ma, Y-S Zeng, H-Y Shen
This corrects the article DOI: 10.1038/sc.2016.36.
July 18, 2017: Spinal Cord
https://www.readbyqxmd.com/read/28715636/backbone-engineered-%C3%AE-peptide-amphitropic-gels-for-immobilization-of-semiconductor-quantum-dots-and-2d-cell-culture
#14
Rajkumar Misra, Aman Sharma, Anjali Shiras, Hosahudya N Gopi
We are reporting a spontaneous supramolecular assembly of backbone engineered γ-peptide scaffold and its utility in the immobilization of semiconductor quantum dots and in cell culture. The stimulating feature of this γ-peptide scaffold is that it efficiently gelate both aqueous phosphate buffers as well as aromatic organic solvents. A comparative and systematic investigation reveals that the greater spontaneous self-aggregation property of γ-peptide over the α-, and β-peptide analogues is mainly due to the backbone flexibility, increased hydrophobicity and "π-π stacking of γ-phenylalanine residues...
July 17, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28715014/amyloid-scaffolds-as-alternative-chlorosomes
#15
REVIEW
Rolando F Rengifo, Noel X Li, Anthony Sementilli, David G Lynn
Living systems contain remarkable functional capability built within sophisticated self-organizing frameworks. Defining the assembly codes that coordinate these systems could greatly extend nanobiotechnology. To that end, we have highlighted the self-assembling architecture of the chlorosome antenna arrays and report the emulation and extension of their features for the development of cell-compatible photoredox materials. We specifically review work on amyloid peptide scaffolds able to (1) organize light-harvesting chromophores, (2) break peptide bilayer symmetry for directional energy and electron transfer, and (3) incorporate redox active metal ions at high density for energy storage...
July 17, 2017: Organic & Biomolecular Chemistry
https://www.readbyqxmd.com/read/28714570/human-osteoarthritic-chondrons-outnumber-patient-and-joint-matched-chondrocytes-in-hydrogel-culture-future-application-in-autologous-cell-based-oa-cartilage-repair
#16
Miriam Rothdiener, Tatiana Uynuk-Ool, Norbert Südkamp, Matthias Aurich, Alan J Grodzinsky, Bodo Kurz, Bernd Rolauffs
Autologous chondrocyte implantation (ACI) is used in 34-60% for osteoarthritic (OA) cartilage defects, although ACI is neither recommended nor designed for OA. Envisioning a hydrogel-based ACI for OA that uses chondrons instead of classically used chondrocytes, we hypothesized that human OA-chondrons may outperform OA-chondrocytes. We compared patient- and joint surface-matched human OA-chondrons vs. OA-chondrocytes cultured for the first time in a hydrogel, using a self-assembling peptide system. We determined yield, viability, cell numbers, mRNA expression, GAPDH mRNA enzyme activity, collagen II synthesis (CPII) and degradation (C2C), and sGAG...
July 17, 2017: Journal of Tissue Engineering and Regenerative Medicine
https://www.readbyqxmd.com/read/28714303/melittin-containing-hybrid-peptide-hydrogels-for-enhanced-photothermal-therapy-of-glioblastoma
#17
Honglin Jin, Guifang Zhao, Jianli Hu, Quanguang Ren, Kui Yang, Chao Wan, Ai Huang, Pindong Li, Jue-Ping Feng, Jing Chen, Zhenwei Zou
The design of biocompatible and efficacious anticancer biomaterials to achieve relatively low tumor recurrence rates is the main pursuit of cancer photothermal therapy (PTT). RADA16-I is a synthetic amphiphilic peptide with the sequence RADARADARADARADA that can self-assemble into a peptide nanofiber hydrogel. In this study, we synthesized a novel melittin-RADA32-indocyanine green (ICG) hydrogel ("MRI hydrogel"), which contains melittin in the peptide hydrogel backbone and ICG in the hydrogel matrix, for enhanced PTT of glioblastomas...
July 25, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28714246/carbon-nanodot-sensitized-modulation-of-alzheimer-s-%C3%AE-amyloid-self-assembly-disassembly-and-toxicity
#18
You Jung Chung, Kayoung Kim, Byung Il Lee, Chan Beum Park
The self-assembly of amyloidogenic peptides into β-sheet-rich aggregates is a general feature of many neurodegenerative diseases, including Alzheimer's disease, which signifies the need for the effective attenuation of amyloid aggregation toward alleviating amyloid-associated neurotoxicity. This study reports that photoluminescent carbon nanodots (CDs) can effectively suppress Alzheimer's β-amyloid (Aβ) self-assembly and function as a β-sheet breaker disintegrating preformed Aβ aggregates. This study synthesizes CDs using ammonium citrate through one-pot hydrothermal treatment and passivates their surface with branched polyethylenimine (bPEI)...
July 17, 2017: Small
https://www.readbyqxmd.com/read/28712055/potential-of-casein-as-a-carrier-for-biologically-active-agents
#19
REVIEW
Tomasz Konrad Głąb, Janusz Boratyński
Casein is the collective name for a family of milk proteins. In bovine milk, casein comprises four peptides: αS1, αS2, β, and κ, differing in their amino acid, phosphorus and carbohydrate content but similar in their amphiphilic character. Hydrophilic and hydrophobic regions of casein show block distribution in the protein chain. Casein peptides carry negative charge on their surface as a result of phosphorylation and tend to bind nanoclusters of amorphous calcium phosphate. Due to these properties, in suitable conditions, casein molecules agglomerate into spherical micelles...
August 2017: Topics in Current Chemistry (Journal)
https://www.readbyqxmd.com/read/28709995/the-contribution-of-biophysical-and-structural-studies-of-protein-self-assembly-to-the-design-of-therapeutic-strategies-for-amyloid-diseases
#20
Nunilo Cremades, Christopher M Dobson
Many neurodegenerative disorders, including Alzheimer's, Parkinson's and the prion diseases, are characterized by a conformational conversion of normally soluble proteins or peptides into pathological species, by a process of misfolding and self-assembly that leads ultimately to the formation of amyloid fibrils. Recent studies support the idea that multiple intermediate species with a wide variety of degrees of neuronal toxicity are generated during such processes. The development of a high level of knowledge of the nature and structure of the pathogenic amyloid species would significantly enhance efforts to underline the molecular origins of these disorders and also to develop both accurate diagnoses and effective therapeutic interventions for these types of conditions...
July 12, 2017: Neurobiology of Disease
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