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peptide self-assembly

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https://www.readbyqxmd.com/read/28930631/direct-evidence-of-intrinsic-blue-fluorescence-from-oligomeric-interfaces-of-human-serum-albumin
#1
Arpan Bhattacharya, Soumitra Bhowmik, Amit K Singh, Prashant Kodgire, Apurba K Das, Tushar Kanti Mukherjee
The molecular origin behind the concentration-dependent intrinsic blue fluorescence of human serum albumin (HSA) is not known yet. This unusual blue fluorescence is believed to be a characteristic feature of amyloid-like fibrils of protein/peptide and originates due to the delocalization of peptide bond electrons through the extended hydrogen bond networks of cross--sheet structure. Herein, by combining the results of spectroscopy, size exclusion chromatography, native gel electrophoresis and confocal microscopy, we have shown that the intrinsic blue fluorescence of HSA exclusively originates from oligomeric interfaces devoid of any amyloid-like fibrillar structure...
September 20, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28929563/morphology-dependent-cell-imaging-by-a-self-assembled-diacetylene-peptide-amphiphile
#2
Carsten Schmuck, Hao Jiang, Xiao-Yu Hu, Stefanie Schlesiger, Mao Li, Elio Zellermann, Shirley Knauer
Herein, a novel cationic peptide gemini amphiphile containing diacetylene motifs (DA2P) is presented which self-assembles into novel tadpole- and bola-shaped nanostructures at low concentration and nanofibers at higher concentration, respectively. Interestingly, the DA2P assemblies can be polymerized to a fluorescent red phase but only during incubation with HeLa cells, most likely owing to the reorganization of the diacetylene chains of DA2P upon interaction with the cell membrane. The red-fluorescent polymerized DA2P assemblies can serve as a novel cell imaging probe...
September 20, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28929517/neural-differentiation-of-bone-marrow-mesenchymal-stem-cells-with-human-brain-derived-neurotrophic-factor-gene-modified-in-functionalized-self-assembling-peptide-hydrogel-in-vitro
#3
Hongbin Luo, Changsheng Xu, Zhiwei Liu, Lin Yang, Yunda Hong, Guisheng Liu, Huifen Zhong, Xinyi Cai, Xuping Lin, Xiaokun Chen, Changsheng Wang, Nanwen Zhang, Weihong Xu
OBJECTIVE: To investigate the biocompatibility and differentiation of human brain-derived neurotrophic factor (hBDNF) gene-modified bone marrow mesenchymal stem cells (hBDNF-rMSCs) in a functionalized self-assembling peptide hydrogel. METHODS: hBDNF were engineered in rMSCs using adenovirus vector and the enhanced green fluorescence protein (eGFP) was used as a reporter gene. MSC-specific surface markers (CD90,CD29 and CD45)were used for identifying rMSCs. Fluorescence microscope was used to detect the transfection of rMSCs...
September 19, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/28925983/structure-dependent-interfacial-properties-of-chaplin-f-from-streptomyces-coelicolor
#4
Mina Dokouhaki, Emma L Prime, Andrew Hung, Greg G Qiao, Li Day, Sally L Gras
Chaplin F (Chp F) is a secreted surface-active peptide involved in the aerial growth of Streptomyces. While Chp E demonstrates a pH-responsive surface activity, the relationship between Chp F structure, function and the effect of solution pH is unknown. Chp F peptides were found to self-assemble into amyloid fibrils at acidic pH (3.0 or the isoelectric point (pI) of 4.2), with ~99% of peptides converted into insoluble fibrils. In contrast, Chp F formed short assemblies containing a mixture of random coil and β-sheet structure at a basic pH of 10...
September 19, 2017: Biomolecules
https://www.readbyqxmd.com/read/28925256/catalytic-activity-of-peptide-nanoparticle-conjugates-regulated-by-a-conformational-change
#5
Dorian Jamal Mikolajczak, Jason Lee Heier, Boris Schade, Beate Koksch
Herein, we present the design and synthesis of a catalytically active peptide-nanoparticle conjugate whose activity is regulated by a defined conformational change in the self-assembled peptide monolayer. A catalytically active peptide, designed after the heterodimeric α-helical coiled-coil principle was immobilized onto gold nanoparticles and kinetic studies were performed according to the Michaelis-Menten model. The formed peptide monolayer at the gold nanoparticle surface accelerated p nitrophenylacetate (pNPA) hydrolysis by one order of magnitude compared to the soluble peptide while exhibiting no defined secondary structure as determined by infrared (IR) and circular dichroism (CD) spectroscopy...
September 19, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28922400/protein-folding-misfolding-and-aggregation-the-importance-of-two-electron-stabilizing-interactions
#6
Andrzej Stanisław Cieplak
Proteins associated with neurodegenerative diseases are highly pleiomorphic and may adopt an all-α-helical fold in one environment, assemble into all-β-sheet or collapse into a coil in another, and rapidly polymerize in yet another one via divergent aggregation pathways that yield broad diversity of aggregates' morphology. A thorough understanding of this behaviour may be necessary to develop a treatment for Alzheimer's and related disorders. Unfortunately, our present comprehension of folding and misfolding is limited for want of a physicochemical theory of protein secondary and tertiary structure...
2017: PloS One
https://www.readbyqxmd.com/read/28920077/mimicking-the-bioactivity-of-fibroblast-growth-factor-2-using-supramolecular-nanoribbons
#7
Charles M Rubert Pérez, Zaida Álvarez, Feng Chen, Taner Aytun, Samuel I Stupp
Fibroblast growth factor (FGF-2) is a multifunctional growth factor that has pleiotropic effects in different tissues and organs. In particular, FGF-2 has a special role in angiogenesis, an important process in development, wound healing, cell survival, and differentiation. Therefore, incorporating biological agents like FGF-2 within therapeutic biomaterials is a potential strategy to create angiogenic bioactivity for the repair of damaged tissue caused by trauma or complications that arise from age and/or disease...
September 11, 2017: ACS Biomaterials Science & Engineering
https://www.readbyqxmd.com/read/28918028/human-dmbt1-derived-cell-penetrating-peptides-for-intracellular-sirna-delivery
#8
Martina Tuttolomondo, Cinzia Casella, Pernille Lund Hansen, Ester Polo, Luciana M Herda, Kenneth A Dawson, Henrik J Ditzel, Jan Mollenhauer
Small interfering RNA (siRNA) is a promising molecule for gene therapy, but its therapeutic administration remains problematic. Among the recently proposed vectors, cell-penetrating peptides show great promise in in vivo trials for siRNA delivery. Human protein DMBT1 (deleted in malignant brain tumor 1) is a pattern recognition molecule that interacts with polyanions and recognizes and aggregates bacteria. Taking advantage of these properties, we investigated whether specific synthetic DMBT1-derived peptides could be used to formulate nanoparticles for siRNA administration...
September 15, 2017: Molecular Therapy. Nucleic Acids
https://www.readbyqxmd.com/read/28915004/calcium-induced-morphological-transitions-in-peptide-amphiphiles-detected-by-19-f-magnetic-resonance-imaging
#9
Adam T Preslar, Laura M Lilley, Kohei Sato, Shanrong Zhang, Zer Keen Chia, Samuel I Stupp, Thomas J Meade
Misregulation of extracellular Ca(2+) can indicate bone-related pathologies. New, noninvasive tools are required to image Ca(2+) fluxes and fluorine magnetic resonance imaging ((19)F-MRI) is uniquely suited to this challenge. Here, we present three, highly fluorinated peptide amphiphiles that self-assemble into nanoribbons in buffered saline and demonstrate these nanostructures can be programmed to change (19)F-NMR signal intensity as a function of Ca(2+) concentration. We determined these nanostructures show significant reduction in (19)F-NMR signal as nanoribbon width increases in response to Ca(2+), corresponding to (19)F-MR image intensity reduction...
September 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28915003/the-evolution-of-dna-templated-synthesis-as-a-tool-for-materials-discovery
#10
Rachel K O'Reilly, Andrew J Turberfield, Thomas R Wilks
Precise control over reactivity and molecular structure is a fundamental goal of the chemical sciences. Billions of years of evolution by natural selection have resulted in chemical systems capable of information storage, self-replication, catalysis, capture and production of light, and even cognition. In all these cases, control over molecular structure is required to achieve a particular function: without structural control, function may be impaired, unpredictable, or impossible. The search for molecules with a desired function is often achieved by synthesizing a combinatorial library, which contains many or all possible combinations of a set of chemical building blocks (BBs), and then screening this library to identify "successful" structures...
September 15, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28913864/strand-displacement-in-coiled-coil-structures-controlled-induction-and-reversal-of-proximity
#11
Katharina Gröger, Georgina Gavins, Oliver Seitz
Coiled-coil peptides are frequently used to create new function upon self-assembly of supramolecular complexes. A multitude of coil peptide sequences provides control over specificity and stability of coiled-coil complexes. However, comparably little attention has been paid to the development of methods that allow for the reversal of complex formation under non-denaturing conditions. Herein, we present a reversible 2-state switching system. The process involves two peptide molecules for formation of a size-mismatched coiled-coil duplex and a third, disruptor peptide which targets an overhanging end...
September 15, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28905963/coarse-grained-molecular-dynamics-studies-of-the-structure-and-stability-of-peptide-based-drug-amphiphile-filaments
#12
Myungshim Kang, Honggang Cui, Sharon M Loverde
Peptide-based supramolecular filaments, in particular filaments self-assembled by drug amphiphiles (DAs), possess great potential in the field of drug delivery. These filaments possess one hundred percent drug loading, with a release mechanism that can be tuned based on the dissociation of the supramolecular filaments and the degradation of the DAs [Cheetham et al., J. Am. Chem. Soc., 2013, 135(8), 2907]. Recently, much attention has been drawn to the competing intermolecular interactions that drive the self-assembly of peptide-based amphiphiles into supramolecular filaments...
September 14, 2017: Soft Matter
https://www.readbyqxmd.com/read/28902999/self-assembled-2d-free-standing-janus-nanosheets-with-single-layer-thickness
#13
Yiyang Lin, Michael R Thomas, Amy Gelmi, Vincent Leonardo, E Thomas Pashuck, Stephanie A Maynard, Ye Wang, Molly M Stevens
We report the thermodynamically controlled growth of solution-processable and free-standing nanosheets via peptide assembly in two dimensions. By taking advantage of self-sorting between peptide β-strands and hydrocarbon chains, we have demonstrated the formation of Janus 2D structures with single-layer thickness, which enable a predetermined surface heterofunctionalization. A controlled 2D-to-1D morphological transition was achieved by subtly adjusting the intermolecular forces. These nanosheets provide an ideal substrate for the engineering of guest components (e...
September 13, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28902961/photoluminescence-of-diphenylalanine-peptide-nano-microstructures-from-mechanisms-to-applications
#14
REVIEW
Zhixing Gan, Hao Xu
Diphenylalanine (Phe-Phe, FF) molecules, which can self-assemble into highly ordered nano/microstructures, have increasingly aroused intense interests due to their special optical properties. In this review, recent advances in photoluminescence (PL) of supramolecular architectures of FF-based peptide and the underlying mechanisms are highlighted. Mainly deep ultraviolet emission at around 285 nm and/or blue emission at ≈450 nm are observed in various FF peptide structures and its derivatives, which are primarily interpreted by quantum confinement effects, shallow radiative traps, and electron delocalization via hydrogen bonds in β-sheet structures...
September 13, 2017: Macromolecular Rapid Communications
https://www.readbyqxmd.com/read/28902203/molecular-engineering-solutions-for-therapeutic-peptide-delivery
#15
REVIEW
Handan Acar, Jeffrey M Ting, Samanvaya Srivastava, James L LaBelle, Matthew V Tirrell
Proteins and their interactions in and out of cells must be well-orchestrated for the healthy functioning and regulation of the body. Even the slightest disharmony can cause diseases. Therapeutic peptides are short amino acid sequences (generally considered <50 amino acids) that can naturally mimic the binding interfaces between proteins and thus, influence protein-protein interactions. Because of their fidelity of binding, peptides are a promising next generation of personalized medicines to reinstate biological harmony...
September 13, 2017: Chemical Society Reviews
https://www.readbyqxmd.com/read/28900511/a-nanosystem-of-amphiphilic-oligopeptide-drug-conjugate-actualizing-both-%C3%AE-v%C3%AE-3-targeting-and-reduction-triggered-release-for-maytansinoid
#16
Yanqin Liang, Suxin Li, Xiaoyou Wang, Bo He, Bing He, Wenbing Dai, Hua Zhang, Xueqing Wang, Yiguang Wang, Demin Zhou, Qiang Zhang
To design a prodrug-based self-assembling nanosystem with both ligand targeting and stimuli-responsive features, and elucidate the superiority of each targeting strategy and the synergistic effect between them, we synthesized four small molecule amphiphilic peptide-drug conjugates (APDCs) using maytansinoid (DM1) as a cytotoxic agent, cRGDfK as a homing peptide, and disulfide (SS) or thioether (SMCC) as linker. Owing to their amphiphilicity, the APDCs could self-assemble into nanoparticles (APDC@NPs) which were evaluated in vitro in three different cell lines and in vivo in tumor-bearing C57BL/6 mice...
2017: Theranostics
https://www.readbyqxmd.com/read/28892645/self-assembling-peptide-p11-4-and-fluoride-for-regenerating-enamel
#17
M Alkilzy, A Tarabaih, R M Santamaria, C H Splieth
Regenerative medicine-based approaches for caries treatment focus on biomimetic remineralization of initial carious lesions as a minimal invasive therapy. In vitro, self-assembling peptide P11-4 enhances remineralization of early carious lesions. To investigate the safety and clinical efficacy of P11-4 for treatment of initial caries, a randomized controlled single-blind study was conducted on children aged >5 y with visible active early caries on erupting permanent molars. Subjects were randomized to either the test group (P11-4 + fluoride varnish) or control group (fluoride varnish alone)...
September 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28891286/conformation-preservation-of-alpha-helical-peptides-within-supramolecular-filamentous-assemblies
#18
Yi Li, Yuzhu Wang, Shih-Hao Ou, Lye Lin Lock, Xuankuo Xu, Sanchayita Ghose, Zheng Jian Li, Honggang Cui
Hydrogen-bonded β-sheets are the most commonly explored building motifs for creating peptide-based filamentous nanostructures, however, most bioactive epitopes must assume an α-helix conformation to exert their functions. Incorporating α-helical sequences into β-sheet-forming peptides often involves the use of a flexible spacer to alleviate the steric impact of the intermolecular hydrogen bonding on the α-helical conformation. In this context, we report our findings on the alkylation-regulated conformation preservation of α-helical peptides within their filamentous assemblies...
September 11, 2017: Biomacromolecules
https://www.readbyqxmd.com/read/28885830/oligoprolines-as-molecular-entities-for-controlling-distance-in-biological-and-material-sciences
#19
Stefanie Dobitz, Matthew R Aronoff, Helma Wennemers
Nature utilizes large biomolecules to fulfill tasks that require spatially well-defined arrangements at the molecular level such as electron transfer, ligand-receptor interactions, or catalysis. The creation of synthetic molecules that enable precise control over spacing and functionalization provides opportunities across diverse disciplines. Key requirements of functionalizable oligomeric scaffolds include the specific control of their molecular properties where the correct balance of flexibility and rigidity must be maintained in addition to the prerequisite of defined length...
September 8, 2017: Accounts of Chemical Research
https://www.readbyqxmd.com/read/28879768/supramolecular-copolymerization-of-short-peptides-and-polyoxometalates-toward-the-fabrication-of-underwater-adhesives
#20
Jing Xu, Xiangyi Li, Xiaodong Li, Bao Li, Lixin Wu, Wen Li, Xiaoming Xie, Rong Xue
Peptide assembly has reached exquisite levels of efficiency in the creation of bioactive materials. However, we have not yet been able to take what we have learned from peptide assembly to develop a general strategy for the fabrication of biomimetic underwater adhesives, which retain significant advantages as medical glue for clinical treatment. Herein we report a simple approach to prepare peptide-based adhesives through the supramolecular polymerization of cationic peptides drove by polyoxometalates (PMs)...
September 15, 2017: Biomacromolecules
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