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https://www.readbyqxmd.com/read/28214460/managing-acetabular-defects-in-total-hip-arthroplasty
#1
Brian Park, Frank Liporace, Scott Marwin
With the aging population and rising incidence of primary total joint arthroplasty has come the increasing incidence of revision total hip arthroplasties. One challenge in revision total hip arthroplasty is dealing with acetabular defects. The orthopaedic surgeon who chooses to take on these challenges requires a proper method for the evaluation of these defects as well as an evidence-based treatment algorithm. Initial assessment requires appropriate use and interpretation of imaging modalities such as x-rays and computed tomography...
January 2017: Bulletin of the Hospital for Joint Diseases
https://www.readbyqxmd.com/read/28196958/transcription-profiling-of-bacillus-subtilis-cells-infected-with-ar9-a-giant-phage-encoding-two-multisubunit-rna-polymerases
#2
Daria Lavysh, Maria Sokolova, Marina Slashcheva, Konrad U Förstner, Konstantin Severinov
Bacteriophage AR9 is a recently sequenced jumbo phage that encodes two multisubunit RNA polymerases. Here we investigated the AR9 transcription strategy and the effect of AR9 infection on the transcription of its host, Bacillus subtilis Analysis of whole-genome transcription revealed early, late, and continuously expressed AR9 genes. Alignment of sequences upstream of the 5' ends of AR9 transcripts revealed consensus sequences that define early and late phage promoters. Continuously expressed AR9 genes have both early and late promoters in front of them...
February 14, 2017: MBio
https://www.readbyqxmd.com/read/28174560/things-are-getting-hairy-enterobacteria-bacteriophage-vb_pcam_cbb
#3
Colin Buttimer, Hanne Hendrix, Hugo Oliveira, Aidan Casey, Horst Neve, Olivia McAuliffe, R Paul Ross, Colin Hill, Jean-Paul Noben, Jim O'Mahony, Rob Lavigne, Aidan Coffey
Enterobacteria phage vB_PcaM_CBB is a "jumbo" phage belonging to the family Myoviridae. It possesses highly atypical whisker-like structures along the length of its contractile tail. It has a broad host range with the capability of infecting species of the genera Erwinia, Pectobacterium, and Cronobacter. With a genome of 355,922 bp, excluding a predicted terminal repeat of 22,456 bp, phage CBB is the third largest phage sequenced to date. Its genome was predicted to encode 554 ORFs with 33 tRNAs. Based on prediction and proteome analysis of the virions, 29% of its predicted ORFs could be functionally assigned...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28109677/effect-of-pulsed-ultrasound-on-the-physicochemical-characteristics-and-emulsifying-properties-of-squid-dosidicus-gigas-mantle-proteins
#4
O A Higuera-Barraza, W Torres-Arreola, J M Ezquerra-Brauer, F J Cinco-Moroyoqui, J C Rodríguez Figueroa, E Marquez-Ríos
Food technologists are always looking to improve the functional properties of proteins. In this sense, in last years ultrasound has been used to improve some functional properties. For this reason, and considering that jumbo squid is an important fishery in northwest Mexico, the purpose of this research was to determine the effect of pulsed ultrasound on the physicochemical characteristics and emulsifying properties of squid (Dosidicus gigas) mantle proteins. Pulsed ultrasound (20kHz, 20, and 40% amplitude) was applied for 30, 60, and 90s to a protein extract prepared from giant squid mantle causing an increase (p<0...
January 12, 2017: Ultrasonics Sonochemistry
https://www.readbyqxmd.com/read/28009054/new-icing-media-for-quality-enhancement-of-chilled-hake-merluccius-merluccius-using-a-jumbo-squid-dosidicus-gigas-skin-extract
#5
Josafat Marina Ezquerra-Brauer, José M Miranda, Jesús Enrique Chan-Higuera, Jorge Barros-Velázquez, Santiago P Aubourg
BACKGROUD: An advanced strategy for chilled fish preservation, based on the inclusion in ice of an extract of jumbo squid (Dosidicus gigas) skin (JSS), is proposed. Aqueous solutions including acetic acid-ethanol extracts of JSS were tested at two different concentrations as icing media, being the effects on the quality evolution of chilled hake (Merluccius merluccius) monitored. RESULTS: A significant inhibition (p < 0.05) of microbial activity (aerobes, psychrotrophs, Enterobacteriaceae, proteolytic bacteria; pH, trimethylamine) was obtained in hake corresponding to the icing batch including the highest JSS concentration...
December 23, 2016: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/27865618/actinidia-arguta-supplementation-protects-aorta-and-liver-in-rats-with-induced-hypercholesterolemia
#6
Maria Leontowicz, Hanna Leontowicz, Iwona Jesion, Wojciech Bielecki, Katarzyna Najman, Piotr Latocha, Yong-Seo Park, Shela Gorinstein
There are no published results focusing on the study of hardy kiwifruit as a supplementation to the atherogenic diet. We hypothesized that hardy kiwifruit (Actinidia arguta (A. arguta)) from Poland possess better pro-healthy action than two Asian varieties (Hayward and Bidan). We tested this hypothesis by measuring the metabolic reactions of rats loaded with 1% cholesterol and supplemented with 5% of hardy kiwifruit (A. arguta), Hayward, or Bidan in their diets. The experiment was performed on 71 male Wistar rats...
November 2016: Nutrition Research
https://www.readbyqxmd.com/read/27807452/child-survival-strategies-assessment-of-knowledge-and-practice-of-rural-women-of-reproductive-age-in-cross-river-state-nigeria
#7
Aniekan Jumbo Etokidem, Ofonime Johnson
Introduction. Nigeria is one of the five countries that account for about 50% of under-five mortality in the world. The objective of this study was to assess the knowledge and practice of child survival strategies among rural community caregivers in Cross River State of Nigeria. Materials and Methods. This descriptive cross-sectional survey used a pretested questionnaire to obtain information from 150 women of reproductive age. Data analysis was done using SPSS version 20. Results. The child survival strategy known to most of the respondents was oral rehydration therapy as indicated by 98% followed by female education by 73...
2016: Journal of Tropical Medicine
https://www.readbyqxmd.com/read/27707469/description-of-plastic-remains-found-in-the-stomach-contents-of-the-jumbo-squid-dosidicus-gigas-landed-in-ecuador-during-2014
#8
Rigoberto Rosas-Luis
Squids are active and opportunistic predators that feed on a wide range of prey. Their active movements in the water column and their voracity promote a high consumption of food. In the pelagic environment off Ecuador, marine pollution is characterized by plastic debris with a mainland origin, including plastics trash of fishing gears. The objective of this work was to describe the presence of plastic remains in the stomach contents of Dosidicus gigas caught off the coast of Ecuador. Results demonstrated that 12% of the stomachs contained plastic remains...
December 15, 2016: Marine Pollution Bulletin
https://www.readbyqxmd.com/read/27663505/the-gastric-acid-pocket-is-attenuated-in-h-pylori-infected-subjects
#9
David R Mitchell, Mohammad H Derakhshan, Angela A Wirz, Clare Orange, Stuart A Ballantyne, James J Going, Kenneth E L McColl
OBJECTIVE: Gastric acid secretory capacity in different anatomical regions, including the postprandial acid pocket, was assessed in Helicobacter pylori positive and negative volunteers in a Western population. DESIGN: We studied 31 H. pylori positive and 28 H. pylori negative volunteers, matched for age, gender and body mass index. Jumbo biopsies were taken at 11 predetermined locations from the gastro-oesophageal junction and stomach. Combined high-resolution pH metry (12 sensors) and manometry (36 sensors) was performed for 20 min fasted and 90 min postprandially...
September 23, 2016: Gut
https://www.readbyqxmd.com/read/27619796/characteristics-and-complete-genome-analysis-of-a-novel-jumbo-phage-infecting-pathogenic-bacillus-pumilus-causing-ginger-rhizome-rot-disease
#10
Yihui Yuan, Meiying Gao
Tailed phages with genomes larger than 200 kbp are classified as jumbo phage and exhibit extremely high diversity. In this study, a novel jumbo phage, vB_BpuM_BpSp, infecting pathogenic Bacillus pumilus, the cause of ginger rhizome rot disease, was isolated. Notable features of phage vB_BpuM_BpSp are the large phage capsid of 137 nm and baseplate-attached curly tail fibers. The genome of the phage is 255,569 bp in size with G+C content of 25.9 %, and it shows low similarity to known biological entities...
December 2016: Archives of Virology
https://www.readbyqxmd.com/read/27588938/the-role-of-water-on-the-structure-and-mechanical-properties-of-a-thermoplastic-natural-block-co-polymer-from-squid-sucker-ring-teeth
#11
Clément Rieu, Luca Bertinetti, Roman Schuetz, Cesar Ca Salinas-Zavala, James C Weaver, Peter Fratzl, Ali Miserez, Admir Masic
Hard biological polymers exhibiting a truly thermoplastic behavior that can maintain their structural properties after processing are extremely rare and highly desirable for use in advanced technological applications such as 3D-printing, biodegradable plastics and robust composites. One exception are the thermoplastic proteins that comprise the sucker ring teeth (SRT) of the Humboldt jumbo squid (Dosidicus gigas). In this work, we explore the mechanical properties of reconstituted SRT proteins and demonstrate that the material can be re-shaped by simple processing in water and at relatively low temperature (below 100 °C)...
2016: Bioinspiration & Biomimetics
https://www.readbyqxmd.com/read/27563033/genome-sequence-of-jumbo-phage-vb_abam_me3-of-acinetobacter-baumanni
#12
Colin Buttimer, Lisa O'Sullivan, Mohamed Elbreki, Horst Neve, Olivia McAuliffe, R Paul Ross, Colin Hill, Jim O'Mahony, Aidan Coffey
Bacteriophage (phage) vB_AbaM_ME3 was previously isolated from wastewater effluent using the propagating host Acinetobacter baumannii DSM 30007. The full genome was sequenced, revealing it to be the largest Acinetobacter bacteriophage sequenced to date with a size of 234,900 bp and containing 326 open reading frames (ORFs).
2016: Genome Announcements
https://www.readbyqxmd.com/read/27562100/acute-and-long-term-outcomes-in-a-drosophila-melanogaster-model-of-classic-galactosemia-occur-independently-of-galactose-1-phosphate-accumulation
#13
Jennifer M I Daenzer, Patricia P Jumbo-Lucioni, Marquise L Hopson, Kerry R Garza, Emily L Ryan, Judith L Fridovich-Keil
Classic galactosemia (CG) is a potentially lethal inborn error of metabolism that results from the profound loss of galactose-1-phosphate uridylyltransferase (GALT), the second enzyme in the Leloir pathway of galactose metabolism. Neonatal detection and dietary restriction of galactose minimizes or resolves the acute sequelae of CG, but fails to prevent the long-term complications experienced by a majority of patients. One of the substrates of GALT, galactose-1-phosphate (Gal-1P), accumulates to high levels in affected infants, especially following milk exposure, and has been proposed as the key mediator of acute and long-term pathophysiology in CG...
November 1, 2016: Disease Models & Mechanisms
https://www.readbyqxmd.com/read/27466186/coordinated-movement-neuromuscular-synaptogenesis-and-trans-synaptic-signaling-defects-in-drosophila-galactosemia-models
#14
Patricia P Jumbo-Lucioni, William M Parkinson, Danielle L Kopke, Kendal Broadie
The multiple galactosemia disease states manifest long-term neurological symptoms. Galactosemia I results from loss of galactose-1-phosphate uridyltransferase (GALT), which converts galactose-1-phosphate + UDP-glucose to glucose-1-phosphate + UDP-galactose. Galactosemia II results from loss of galactokinase (GALK), phosphorylating galactose to galactose-1-phosphate. Galactosemia III results from the loss of UDP-galactose 4'-epimerase (GALE), which interconverts UDP-galactose and UDP-glucose, as well as UDP-N-acetylgalactosamine and UDP-N-acetylglucosamine...
September 1, 2016: Human Molecular Genetics
https://www.readbyqxmd.com/read/27267368/transcriptomic-profiling-of-melon-necrotic-spot-virus-infected-melon-plants-revealed-virus-strain-and-plant-cultivar-specific-alterations
#15
Cristina Gómez-Aix, Laura Pascual, Joaquín Cañizares, María Amelia Sánchez-Pina, Miguel A Aranda
BACKGROUND: Viruses are among the most destructive and difficult to control plant pathogens. Melon (Cucumis melo L.) has become the model species for the agriculturally important Cucurbitaceae family. Approaches that take advantage of recently developed genomic tools in melon have been extremely useful for understanding viral pathogenesis and can contribute to the identification of target genes for breeding new resistant cultivars. In this work, we have used a recently described melon microarray for transcriptome profiling of two melon cultivars infected with two strains of Melon necrotic spot virus (MNSV) that only differ on their 3'-untranslated regions...
2016: BMC Genomics
https://www.readbyqxmd.com/read/27242758/proteomic-analysis-of-a-novel-bacillus-jumbo-phage-revealing-glycoside-hydrolase-as-structural-component
#16
Yihui Yuan, Meiying Gao
Tailed phages with genomes of larger than 200 kbp are classified as Jumbo phages and exhibited extremely high uncharted diversity. The genomic annotation of Jumbo phage is often disappointing because most of the predicted proteins, including structural proteins, failed to make good hits to the sequences in the databases. In this study, 23 proteins of a novel Bacillus Jumbo phage, vB_BpuM_BpSp, were identified as phage structural proteins by the structural proteome analysis, including 14 proteins of unknown function, 5 proteins with predicted function as structural proteins, a glycoside hydrolase, a Holliday junction resolvase, a RNA-polymerase β-subunit, and a host-coding portal protein, which might be hijacked from the host strain during phage virion assembly...
2016: Frontiers in Microbiology
https://www.readbyqxmd.com/read/27196581/hiatus-hernia-in-healthy-volunteers-is-associated-with-intrasphincteric-reflux-and-cardiac-mucosal-lengthening-without-traditional-reflux
#17
Elaine V Robertson, Mohammad H Derakhshan, Angela A Wirz, David R Mitchell, James J Going, Andrew W Kelman, Stuart A Ballantyne, Kenneth E L McColl
BACKGROUND AND AIMS: Hiatus hernia (HH) is a key mediator of gastro-oesophageal reflux disease but little is known about its significance in the general population. We studied the structure and function of the gastro-oesophageal junction in healthy volunteers with and without HH. METHODS: We compared 15 volunteers with HH, detected by endoscopy or MRI scan, but without gastro-oesophageal reflux disease with 15 controls matched for age, gender and body weight. Jumbo biopsies were taken across the squamocolumnar junction (SCJ)...
April 25, 2016: Gut
https://www.readbyqxmd.com/read/27081857/two-asian-jumbo-phages-%C3%AF-rsl2-and-%C3%AF-rsf1-infect-ralstonia-solanacearum-and-show-common-features-of-%C3%AF-kz-related-phages
#18
Anjana Bhunchoth, Romain Blanc-Mathieu, Tomoko Mihara, Yosuke Nishimura, Ahmed Askora, Namthip Phironrit, Chalida Leksomboon, Orawan Chatchawankanphanich, Takeru Kawasaki, Miyako Nakano, Makoto Fujie, Hiroyuki Ogata, Takashi Yamada
Jumbo phages infecting Ralstonia solanacearum were isolated in Thailand (ϕRSL2) and Japan (ϕRSF1). They were similar regarding virion morphology, genomic arrangement, and host range. Phylogenetic and proteomic tree analyses demonstrate that the ϕRSL2 and ϕRSF1 belong to a group of evolutionary related phages, including Pseudomonas phages ϕKZ, 201ϕ2-1 and all previously described ϕKZ-related phages. Despite conserved genomic co-linearity between the ϕRSL2 and ϕRSF1, they differ in protein separation patterns...
July 2016: Virology
https://www.readbyqxmd.com/read/27047229/clinical-evaluation-and-surgical-options-in-acetabular-reconstruction-a-literature-review
#19
REVIEW
Asim Qamar Ahmad, Ran Schwarzkopf
The purpose of this paper is to review the clinical indications for acetabular reconstruction in patients with underlying peri-prosthetic segmental and cavitary defects, evaluate steps in pre-operative planning, and present the American Academy of Orthopaedic Surgeons (AAOS) and Paprosky classification systems to categorize acetabular defects. We also present a review of the current surgical techniques to reconstruct the acetabular socket which includes a cementless acetabular component with morselized bone, structural allograft, jumbo and oblong cups, reinforcement rings, bone cages, custom triflange acetabular constructs, and trabecular metal components...
December 2015: Journal of Orthopaedics
https://www.readbyqxmd.com/read/27042202/apoptosis-inducing-effects-of-jujube-z%C3%A7-o-seed-extracts-on-human-jurkat-leukemia-t-cells
#20
Natthanan Taechakulwanijya, Natthida Weerapreeyakul, Sahapat Barusrux, Sirithorn Siriamornpun
BACKGROUND: Jujube (Zǎo) seeds exhibited anticancer effects and used in Chinese medicine for many years. This study aims to investigate the apoptosis-inducing effects of seed extracts from eight different cultivated species ('Apple', 'Bombay', 'Jumbo', 'Kaew', 'Nomsod', 'Rianthong', 'Samros', and 'Taiwan') on human Jurkat leukemia T cells. METHODS: We evaluated the effects of seed extracts from eight jujube cultivated species on human Jurkat leukemia T cells. The crude seed extracts were prepared sequentially by using water, 95 % ethanol, dichloromethane, ethyl acetate, chloroform or hexane...
2016: Chinese Medicine
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