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https://www.readbyqxmd.com/read/29786519/effect-of-vaccination-on-cattle-subclinically-infected-with-foot-and-mouth-disease-virus-in-cameroon
#1
Miranda R Bertram, Amy Delgado, Steven J Pauszek, George R Smoliga, Barbara Brito, Carolina Stenfeldt, Ethan J Hartwig, Simon Dickmu Jumbo, Mamoudou Abdoulmoumini, Amba Abona Oliva Marie, Robert Salhine, Luis L Rodriguez, Rebecca Garabed, Jonathan Arzt
Foot-and-mouth disease (FMD) is one of the most contagious and economically important livestock diseases worldwide. Four serotypes of FMD virus (FMDV; O, A, SAT1, SAT2) circulate in Cameroon, and a trivalent inactivated vaccine against the three most common serotypes (O, A, SAT2) was recently introduced in 2014. The objective of this study was to characterize vaccine performance in cattle under natural hyperendemic conditions in the Adamawa region of Cameroon. Vaccinated cattle (n = 50) and non-vaccinated controls (n = 100) were monitored by serum and oropharyngeal fluid (OPF) sample collection through a 12-month period...
July 1, 2018: Preventive Veterinary Medicine
https://www.readbyqxmd.com/read/29777834/erwinia-amylovora-phage-vb_eamm_y3-represents-another-lineage-of-hairy-myoviridae
#2
Colin Buttimer, Yannick Born, Alan Lucid, Martin J Loessner, Lars Fieseler, Aidan Coffey
To date, a small number of jumbo myoviruses have been reported to possess atypical whisker-like structures along the surface of their contractile tails. Erwinia amylovora phage vB_EamM_Y3 is another example. It possesses a genome of 261,365 kbp with 333 predicted ORFs. Using a combination of BLASTP, Interproscan and HHpred, about 21% of its putative proteins could be assigned functions involved in nucleotide metabolism, DNA replication, virion structure and cell wall degradation. The phage was found to have a signal-arrest-release (SAR) endolysin (Y3_301) possessing a soluble lytic transglycosylase domain...
May 16, 2018: Research in Microbiology
https://www.readbyqxmd.com/read/29773962/discovery-and-validation-of-genomic-regions-associated-with-resistance-to-maize-lethal-necrosis-in-four-biparental-populations
#3
Manje Gowda, Yoseph Beyene, Dan Makumbi, Kassa Semagn, Michael S Olsen, Jumbo M Bright, Biswanath Das, Stephen Mugo, L M Suresh, Boddupalli M Prasanna
In sub-Saharan Africa, maize is the key determinant of food security for smallholder farmers. The sudden outbreak of maize lethal necrosis (MLN) disease is seriously threatening the maize production in the region. Understanding the genetic basis of MLN resistance is crucial. In this study, we used four biparental populations applied linkage mapping and joint linkage mapping approaches to identify and validate the MLN resistance-associated genomic regions. All populations were genotyped with low to high density markers and phenotyped in multiple environments against MLN under artificial inoculation...
2018: Molecular Breeding: New Strategies in Plant Improvement
https://www.readbyqxmd.com/read/29729419/potential-role-for-microrna-in-regulating-hypoxia-induced-metabolic-suppression-in-the-jumbo-squid
#4
Hanane Hadj-Moussa, Samantha M Logan, Brad A Seibel, Kenneth B Storey
At night, Humboldt squid (Dosidicus gigas) rise to the ocean's surface to feed, but come morning, they descend into the ocean's oxygen minimum zone where they can avoid predators but must deal with severe hypoxia, high pressure, and very cold water. To survive this extreme environment, squid use various adaptations to enter a hypometabolic state characterized by metabolic rate suppression by 35-52%, relative to normoxic conditions. The molecular mechanisms facilitating this metabolic flexibility have yet to be elucidated in hypometabolic squid...
May 2, 2018: Biochimica et Biophysica Acta
https://www.readbyqxmd.com/read/29698564/spatial-and-trophic-preferences-of-jumbo-squid-dosidicus-gigas-d%C3%A2-orbigny-1835-in-the-central-gulf-of-california-ecological-inferences-using-stable-isotopes
#5
L D Trasviña-Carrillo, A Hernández-Herrera, Y E Torres-Rojas, F Galván-Magaña, A Sánchez-González, S Aguíñiga-García
RATIONALE: The jumbo squid Dosidicus gigas is a fishery resource of considerable economic and ecological importance in the Mexican Pacific. Studies on its habitat preferences are needed to understand recent fluctuations in the species' abundance and availability. Stable isotope analysis allows us to infer ecological aspects such as spatial distribution and trophic preferences. METHODS: We used an isotope ratio mass spectrometer, automated for carbonate analysis, and coupled to an elemental analyzer, to determine the isotopic composition of statoliths (δ18 O and δ13 C values) and beaks (δ13 C and δ15 N values) from 219 individuals caught over two fishing seasons (2007 and 2009) off the coast of Santa Rosalía, in the central Gulf of California...
April 26, 2018: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29628686/treatment-options-for-chronic-pelvic-discontinuity
#6
REVIEW
Mark D Hasenauer, Wayne G Paprosky, Neil P Sheth
Chronic pelvic discontinuity is a distinct and unique challenge seen during revision total hip arthroplasty (THA) in which the superior ilium is separated from the inferior ischiopubic segment through the acetabulum, rendering the anterior and posterior columns discontinuous. The operative management of acetabular bone loss in revision THA is one of the most difficult challenges today. Common treatment options include cage reconstruction with bulk acetabular allograft, custom triflange acetabular component, a cup-cage construct, jumbo acetabular cup with porous metal augments, or acetabular distraction with a porous tantalum shell with or without modular porous augments...
January 2018: Journal of Clinical Orthopaedics and Trauma
https://www.readbyqxmd.com/read/29564104/delmopinol-hydrochloride-reduces-salmonella-on-cantaloupe-surfaces
#7
Raúl O Saucedo-Alderete, Joseph D Eifert, Renee R Boyer, Robert C Williams, Gregory E Welbaum
Since the surfaces of cantaloupes are highly rough or irregular, bacteria can easily attach and become difficult to remove. Appropriate postharvest washing and sanitizing procedures can help control Salmonella and other pathogens on cantaloupe or other melons during postharvest operations. Delmopinol hydrochloride (delmopinol) is a cationic surfactant that is effective for treating and preventing gingivitis and periodontitis. The application of delmopinol to two cantaloupe cultivars was evaluated for reducing the level of inoculated Salmonella ...
March 2018: Food Science & Nutrition
https://www.readbyqxmd.com/read/29540765/xanthomonas-citri-jumbo-phage-xacn1-exhibits-a-wide-host-range-and-high-complement-of-trna-genes
#8
Genki Yoshikawa, Ahmed Askora, Romain Blanc-Mathieu, Takeru Kawasaki, Yanze Li, Miyako Nakano, Hiroyuki Ogata, Takashi Yamada
Xanthomonas virus (phage) XacN1 is a novel jumbo myovirus infecting Xanthomonas citri, the causative agent of Asian citrus canker. Its linear 384,670 bp double-stranded DNA genome encodes 592 proteins and presents the longest (66 kbp) direct terminal repeats (DTRs) among sequenced viral genomes. The DTRs harbor 56 tRNA genes, which correspond to all 20 amino acids and represent the largest number of tRNA genes reported in a viral genome. Codon usage analysis revealed a propensity for the phage encoded tRNAs to target codons that are highly used by the phage but less frequently by its host...
March 14, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29529665/tantalum-components-in-difficult-acetabular-revisions-have-good-survival-at-5-to-10-years-longer-term-followup-of-a-previous-report
#9
Paul F Lachiewicz, Jane Anne O'Dell
BACKGROUND: The best method for acetabular revisions in patients with severe bone loss remains controversial; typical approaches include jumbo cups, cages, cup-cages, and custom components. Tantalum (TM) components have good results at midterm followup, but the longer term survival and complications are not available. We previously reported on a series of such reconstructions; here we provide additional followup on that group along with a larger study cohort. QUESTIONS/PURPOSES: (1) What is the survival at a minimum followup of 5 years of these components implanted in difficult revisions and what is the mechanism of failure? (2) What is the most common complication of these reconstructions, and what hip scores did these patients achieve? METHODS: Between 2001 and 2010, one surgeon performed 150 acetabular revisions, of which 74 (49%) were done using TM components...
February 2018: Clinical Orthopaedics and related Research
https://www.readbyqxmd.com/read/29499348/continuous-manufacturing-and-analytical-characterization-of-fixed-dose-multilayer-orodispersible-films
#10
Yasmin Thabet, Dominique Lunter, Joerg Breitkreutz
Various drug therapies require more than one active pharmaceutical ingredient (API) for an effective treatment. There are many advantages, e.g. to improve the compliance or pharmacodynamic response in comparison to a monotherapy or to increase the therapy safety. Until now, there are only a few products available for the paediatric population due to the lack of age appropriate dosage forms or studies proving the efficacy and safety of these products. This study aims to develop orodispersible films (ODFs) in a continuous solvent casting process as child appropriate dosage form containing both enalapril maleate (EM) and hydrochlorothiazide (HCT) separated in different film layers...
May 30, 2018: European Journal of Pharmaceutical Sciences
https://www.readbyqxmd.com/read/29396997/potential-use-of-stable-isotope-and-fatty-acid-analyses-for-traceability-of-geographic-origins-of-jumbo-squid-dosidicus-gigas
#11
Yi Gong, Yunkai Li, Xinjun Chen, Ling Chen
RATIONALE: Squid is an important seafood resource for Asian and European countries. With the continuous development of processed squid products, an effective traceability system has become increasingly prominent. Here, we attempt to trace the fishery products of the main target species, jumbo squid (Dosidicus gigas), by using biochemical tracers. METHODS: Carbon and nitrogen isotope ratios (δ13 C and δ15 N values) and fatty acid profiles were identified in squid from three harvest locations in the eastern Pacific Ocean by isotope ratio mass spectrometry and gas chromatography/mass spectrometry, respectively...
April 15, 2018: Rapid Communications in Mass Spectrometry: RCM
https://www.readbyqxmd.com/read/29156552/diversity-of-dsdna-viruses-in-a-south-african-hot-spring-assessed-by-metagenomics-and-microscopy
#12
Olivier Zablocki, Leonardo Joaquim van Zyl, Bronwyn Kirby, Marla Trindade
The current view of virus diversity in terrestrial hot springs is limited to a few sampling sites. To expand our current understanding of hot spring viral community diversity, this study aimed to investigate the first African hot spring (Brandvlei hot spring; 60 °C, pH 5.7) by means of electron microscopy and sequencing of the virus fraction. Microscopy analysis revealed a mixture of regular- and 'jumbo'-sized tailed morphotypes ( Caudovirales ), lemon-shaped virions ( Fuselloviridae- like; salterprovirus-like) and pleiomorphic virus-like particles...
November 18, 2017: Viruses
https://www.readbyqxmd.com/read/29089853/effect-of-amidated-low-methoxyl-pectin-on-physicochemical-characteristics-of-jumbo-squid-dosidicus-gigas-mantle-muscle-gels
#13
Juan C Ramirez-Suarez, Andrés Álvarez-Armenta, Guillermina García-Sánchez, Ramón Pacheco-Aguilar, Susana M Scheuren-Acevedo, Miguel A Mazorra-Manzano, Agustín Rascón-Chu
Jumbo squid ( Dosidicus gigas ) muscle proteins show low functionality with limited use in gel products. This work aims to assess the influence of adding the natural and commercially available fibre, amidated low-methoxyl pectin (at 0.5, 1.0, 1.5, 2.0 and 3.0%), on the physicochemical and functional characteristics of jumbo squid ( Dosidicus gigas ) mantle muscle gels. The addition of 0.5% fibre showed an immediate effect on the gel texture profile analysis, improving hardness (p<0.05) from (3.4±0.7) N of the control (no added fibre) to (5...
September 2017: Food Technology and Biotechnology
https://www.readbyqxmd.com/read/29073989/drug-policy-in-the-czech-republic
#14
Jana Skoupá
BACKGROUND: The legal background of the current pharmaceutical pricing and reimbursement (P&R) setting in the Czech Republic is based on Act 48/1997. Since 2008, the P&R process has been coordinated by the State Institute for Drug Control, which is the main stakeholder in the decision-making process; marketing authorization holders and insurance funds (IFs) also participate. OBJECTIVES: To present a general overview of the current Czech health care system and its P&R principles...
September 2017: Value in Health Regional Issues
https://www.readbyqxmd.com/read/29042498/capsids-and-genomes-of-jumbo-sized-bacteriophages-reveal-the-evolutionary-reach-of-the-hk97-fold
#15
Jianfei Hua, Alexis Huet, Carlos A Lopez, Katerina Toropova, Welkin H Pope, Robert L Duda, Roger W Hendrix, James F Conway
Large icosahedral viruses that infect bacteria represent an extreme of the coevolution of capsids and the genomes they accommodate. One subset of these large viruses is the jumbophages, tailed phages with double-stranded DNA genomes of at least 200,000 bp. We explored the mechanism leading to increased capsid and genome sizes by characterizing structures of several jumbophage capsids and the DNA packaged within them. Capsid structures determined for six jumbophages were consistent with the canonical phage HK97 fold, and three had capsid geometries with novel triangulation numbers (T=25, T=28, and T=52)...
October 17, 2017: MBio
https://www.readbyqxmd.com/read/28993083/acetabular-revision-arthroplasty-using-an-uncemented-deep-profile-jumbo-component-a-ten-to-sixteen-year-follow-up-study
#16
Jeffrey R McLaughlin, Kyla R Lee
BACKGROUND: The purpose of this study is to evaluate the outcome of revision total hip arthroplasty using an uncemented deep profile jumbo acetabular component in patients who had been followed for a minimum of 10 years postoperatively. METHODS: Between 1997 and 2001, 61 revision total hip arthroplasties were performed in 58 patients, with use of the +5 Deep Profile acetabular shell. The outcome with regard to retention vs re-revision of the acetabular component was determined for every hip...
February 2018: Journal of Arthroplasty
https://www.readbyqxmd.com/read/28982391/the-impact-of-early-thromboelastography-directed-therapy-in-trauma-resuscitation
#17
Mohamed Mohamed, Karl Majeske, Gul R Sachwani, Kristin Kennedy, Mina Salib, Michael McCann
BACKGROUND: Conventional coagulation tests do not provide an accurate representation of the complex nature of trauma induced coagulopathy. Thrombelastography provides a prompt global overview of all dynamic sequential aspects of trauma induced coagulopathy. The objective of this study was to evaluate the impact of using thrombelastography on blood products utilization, crystalloids utilization, hospital, and intensive care using length of stay, and cost savings. METHODS: We retrospectively reviewed 134 patients (May of 2012 to February of 2015) meeting Class I trauma activation...
October 5, 2017: Scandinavian Journal of Trauma, Resuscitation and Emergency Medicine
https://www.readbyqxmd.com/read/28955680/acetabular-cup-revision
#18
EDITORIAL
Young-Ho Kim
The use of acetabular cup revision arthroplasty is on the rise as demands for total hip arthroplasty, improved life expectancies, and the need for individual activity increase. For an acetabular cup revision to be successful, the cup should gain stable fixation within the remaining supportive bone of the acetabulum. Since the patient's remaining supportive acetabular bone stock plays an important role in the success of revision, accurate classification of the degree of acetabular bone defect is necessary. The Paprosky classification system is most commonly used when determining the location and degree of acetabular bone loss...
September 2017: Hip & Pelvis
https://www.readbyqxmd.com/read/28946369/the-effects-of-different-dry-roast-parameters-on-peanut-quality-using-an-industrial-belt-type-roaster-simulator
#19
Xiaolei Shi, Lisa O Dean, Jack P Davis, K P Sandeep, Timothy H Sanders
Peanuts roasted to equivalent surface colors at different temperature/time combinations can vary substantially in chemical and physical properties related to product quality. This study used a pilot plant scale roaster that simulates the configurations of one of the most common industrial roaster, a multi-zone belt roaster. Jumbo-size runner-type peanuts were systematically roasted at 5 temperatures (149-204°C) to three Hunter l-values of 53, 48.5, and 43, corresponding to light, medium, and dark roasts. Moisture and tocopherol contents were more closely correlated with roast color rather than temperature, with exceptions at 149°C...
February 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/28945109/reduction-of-syndecan-transcript-levels-in-the-insulin-producing-cells-affects-glucose-homeostasis-in-adult-drosophila-melanogaster
#20
Jonathan L Warren, Eneida Hoxha, Patricia Jumbo-Lucioni, Maria De Luca
Signaling by direct cell-matrix interactions has been shown to impact the transcription, secretion, and storage of insulin in mammalian β cells. However, more research is still needed in this area. Syndecans are transmembrane heparan sulfate proteoglycans that function independently and in synergy with integrin-mediated signaling to mediate cell adhesion to the extracellular matrix. In this study, we used the model organism Drosophila melanogaster to determine whether knockdown of the Syndecan (Sdc) gene expression specifically in the insulin-producing cells (IPCs) might affect insulin-like peptide (ILP) production and secretion...
November 2017: DNA and Cell Biology
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