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https://www.readbyqxmd.com/read/29236325/aml1-eto-trans-activates-c-kit-expression-through-the-long-range-interaction-between-promoter-and-intronic-enhancer
#1
Ying Tian, Genjie Wang, Qingzhu Hu, Xichun Xiao, Shuxia Chen
The AML1/ETO onco-fusion protein is crucial for the genesis of t(8;21) acute myeloid leukemia (AML) and is well documented as a transcriptional repressor through dominant-negative effect. However, little is known about the transactivation mechanism of AML1/ETO. Through large cohort of patient's expression level data analysis and a series of experimental validation, we report here that AML1/ETO transactivates c-KIT expression through directly binding to and mediating the long-range interaction between the promoter and intronic enhancer regions of c-KIT...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29236299/apicomplexa-specific-%C3%AE-hairpin-of-plasmodium-and-toxoplasma-profilins-a-molecular-dynamics-simulation-study
#2
Priyadarsini Kadirvel, Sharmila Anishetty
Profilin is one of the actin-binding proteins that regulate dynamics of actin polymerization. It plays a key role in cell motility and invasion. It also interacts with several other proteins notably through its poly-L-proline (PLP) binding site. Profilin in apicomplexa is characterized by a unique mini-domain consisting of a large β-hairpin extension and an acidic loop which is relatively longer in Plasmodium species. Profilin is essential for the invasive blood stages of Plasmodium falciparum. In the current study, unbound profilins from Plasmodium falciparum (Pf), Toxoplasma gondii (Tg) and Homo sapiens (Hs) were subjected to molecular dynamics (MD) simulations for a timeframe of 100 ns each to understand the conformational dynamics of these proteins...
December 13, 2017: Journal of Cellular Biochemistry
https://www.readbyqxmd.com/read/29236173/is-surgical-plume-developing-during-routine-leeps-contaminated-with-high-risk-hpv-a-pilot-series-of-experiments
#3
Kay Neumann, Markus Cavalar, Achim Rody, Luisa Friemert, Daniel A Beyer
INTRODUCTION: Growing evidence shows a causal role of high-risk humane papillomavirus (HPV) infections in the development of head and neck cancer. A recent case report shows two patients suffering from tonsillar cancer without any risk factors apart from their work as gynecologists doing laser ablations and loop electrosurgical excision procedures (LEEP). The aim of the present investigation is to evaluate whether surgical plume resulting from routine LEEPs of HSIL of the cervix uteri might be contaminated with the DNA of high-risk HPV...
December 13, 2017: Archives of Gynecology and Obstetrics
https://www.readbyqxmd.com/read/29236087/fiber-fabry-perot-force-sensor-with-small-volume-and-high-performance-for-assessing-fretting-damage-of-steam-generator-tubes
#4
Peijian Huang, Ning Wang, Junying Li, Yong Zhu, Jie Zhang
Measuring the radial collision force between the steam generator tube (SGT) and the tube support plate (TSP) is essential to assess the fretting damage of the SGT. In order to measure the radial collision force, a novel miniaturized force sensor based on fiber Fabry-Perot (F-P) was designed, and the principle and characteristics of the sensor were analyzed in detail. Then, the F-P force sensor was successfully fabricated and calibrated, and the overall dimensions of the encapsulated fiber F-P sensor were 17 mm × 5 mm × 3 mm (L × W × H)...
December 13, 2017: Sensors
https://www.readbyqxmd.com/read/29236048/adaptive-wavelet-coding-applied-in-a-wireless-control-system
#5
Felipe O S Gama, Luiz F Q Silveira, Andrés O Salazar
Wireless control systems can sense, control and act on the information exchanged between the wireless sensor nodes in a control loop. However, the exchanged information becomes susceptible to the degenerative effects produced by the multipath propagation. In order to minimize the destructive effects characteristic of wireless channels, several techniques have been investigated recently. Among them, wavelet coding is a good alternative for wireless communications for its robustness to the effects of multipath and its low computational complexity...
December 13, 2017: Sensors
https://www.readbyqxmd.com/read/29236020/application-of-response-surface-methodology-as-a-new-pid-tuning-method-in-an-electrocoagulation-process-control-case
#6
Ş Camcıoğlu, B Özyurt, I C Doğan, H Hapoğlu
In this work the application of response surface methodology (RSM) to proportional-integral-derivative (PID) controller parameter tuning for electrocoagulation (EC) treatment of pulp and paper mill wastewater was researched. Dynamic data for two controlled variables (pH and electrical conductivity) were obtained under pseudo random binary sequence (PRBS) input signals applied to manipulated variables (acid and supporting electrolyte flow rates). Third order plus time delay model parameters were evaluated through System Identification Toolbox™ in MATLAB®...
December 2017: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29235861/kras-switch-mutants-d33e-and-a59g-crystallize-in-the-state-1-conformation
#7
Jia Lu, Asim Bera, Sudershan R Gondi, Kenneth Dale Westover
KRAS switch loop movements play a crucial role in regulating RAS signaling and alteration in these sensitive dynamics are a principal mechanism through which disease-associated RAS mutations lead to aberrant RAS activation. Prior studies suggest that despite high sequence similarity, the switches in KRAS are more dynamic than HRAS. We solved x-ray crystal structures of the rare tumorigenic KRAS mutants KRASD33E, in switch 1 (SW1), and KRASA59G, in switch 2 (SW2), bound to GDP and found these adopt nearly identical, open SW1 conformations as well as altered SW2 conformations...
December 13, 2017: Biochemistry
https://www.readbyqxmd.com/read/29235591/beaming-lamp-single-molecule-capture-and-on-bead-isothermal-amplification-for-digital-detection-of-hepatitis-c-virus-in-plasma
#8
Jiyun Chen, Xiaomin Xu, Zhimei Huang, Yuan Luo, Lijuan Tang, Jian-Hui Jiang
A novel dNAD platform (BEAMing LAMP) by combining emulsion micro-reactors, single-molecule magnetic capture and on-bead loop-mediated isothermal amplification has been developed for DNA detection, which enables absolute and high-precision quantification of a target with a detection limit of 300 copies.
December 13, 2017: Chemical Communications: Chem Comm
https://www.readbyqxmd.com/read/29235423/review-of-a-commercially-available-hybrid-closed-loop-insulin-delivery-system-in-the-treatment-of-type-1-diabetes
#9
Jenine Y Stone, Nalani Haviland, Timothy S Bailey
Type 1 diabetes is an important medical condition causing significant burden and morbidity to those persons affected by it. Improvements in insulin products, insulin delivery and glucose monitoring technology have all contributed to reductions in long-term complications and hypoglycemia. This article reviews the Medtronic 670G device and summarizes the data supporting how this product reduces the burden and increases the safety of insulin dosing in Type 1 diabetes.
December 13, 2017: Therapeutic Delivery
https://www.readbyqxmd.com/read/29235404/understanding-the-interactions-of-different-substrates-with-wild-type-and-mutant-acylaminoacyl-peptidase-using-molecular-dynamics-simulations
#10
Jingxuan Zhu, Yan Wang, Xin Li, Weiwei Han, Li Zhao
Acylaminoacylpeptidase (AAP) belongs to peptidase protein family (POP), which can degrade amyloid β-peptide forms in the brains of patients and hence leads to Alzheimer's disease. And so, AAP is considered to be a novel target in the design of drugs against Alzheimer's disease. In this investigation, six molecular dynamics simulations were used to find that the interaction between the wild-type and R526V AAP with two different substrates (p-nitrophenylcaprylate and Ac-Leu-p-nitroanilide). Our results were as follows: firstly, Ac-Leu-p-nitroanilide bound to R526V AAP to form a more disordered loop (residues 552-562) in the α/β-hydrolase fold like of AAP, which caused an open and inactive AAP domain form, secondly, binding p-nitrophenylcaprylate and Ac-Leu-p-nitroanilide to AAP can decrease the flexibility of residues 225-250, 260-270 and 425-450, in which the ordered secondary structures may contain the suitable geometrical structure and so it is useful to serine attack...
December 13, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/29235170/scaling-up-integrated-early-childhood-development-programs-lessons-from-four-countries
#11
R Pérez-Escamilla, V Cavallera, M Tomlinson, T Dua
BACKGROUND: There is still limited knowledge regarding the translation of early child development (ECD) knowledge into effective policies and large-scale programmes. A variety of frameworks that outline the key steps in scaling up exist, but we argue that taking a complex adaptive systems (CAS) approach assists in understanding the complex, dynamic processes that result in programmes being taken to scale. OBJECTIVES: The objective of this study is to examine the process of scaling up four major country-level ECD programmes through the application of a CAS framework...
January 2018: Child: Care, Health and Development
https://www.readbyqxmd.com/read/29235092/bumetanide-blocks-the-acquisition-of-conditioned-fear-in-adult-rats
#12
Meng-Chang Ko, Ming-Chung Lee, Tso-Hao Tang, Tamara G Amstislavskaya, Maria A Tikhonova, Yi-Ling Yang, Kwok-Tung Lu
BACKGROUND AND PURPOSE: Administration of bumetanide has anxiolytic effects in rat models of conditioned fear. As a loop diuretic, bumetanide may act as an anxiolytic by modulating GABAergic synaptic transmission through the antagonism of cation-chloride cotransporters (CCCs). Its clinical potential for the treatment of anxiety disorders deserves further investigation. In this study, we evaluated the possible role of the basolateral nucleus of amygdala (BLA) in the anxiolytic effect of bumetanide...
December 12, 2017: British Journal of Pharmacology
https://www.readbyqxmd.com/read/29235009/duodenal-exclusion-but-not-sleeve-gastrectomy-preserves-insulin-secretion-making-it-the-more-effective-metabolic-procedure
#13
Claudia Laessle, Gergana Nenova, Goran Marjanovic, Gabriel Seifert, Lampros Kousoulas, Bernd Jaenigen, Stefan Fichtner-Feigl, Jodok Matthias Fink
INTRODUCTION: There is an ongoing debate on which procedure provides the best treatment for type 2 diabetes. Furthermore, the pathomechanisms of diabetes improvement of partly anatomically differing operations is not fully understood. METHODS: A loop duodenojejunostomy (DJOS) with exclusion of one third of intestinal length, a sleeve gastrectomy (SG), or a combination of DJOS + SG was performed in 8-week-old male ZDF rats. One, three, and six months after surgery, an oral glucose tolerance test and measurements of GLP-1, GIP, insulin, and bile acids were conducted...
December 12, 2017: Obesity Surgery
https://www.readbyqxmd.com/read/29234952/increased-calcium-uptake-and-improved-trabecular-bone-properties-in-intestinal-alkaline-phosphatase-knockout-mice
#14
Lucas R Brun, M Lombarte, S Roma, F Perez, J L Millán, A Rigalli
Previous studies have demonstrated a negative correlation between intestinal alkaline phosphatase (IAP) activity and calcium (Ca) absorption in the gut, as IAP acts as a protective mechanism inhibiting high Ca entry into enterocytes, preventing Ca overload. Here we evaluated Ca absorption and bone properties in knockout mice (KO) completely devoid of duodenal IAP (Akp3 -/- mice). Female C57BL/6 control mice (WT, n = 7) and KO mice (n = 10) were used to determine Ca absorption in vivo and by in situ isolated duodenal loops followed by histomorphometric analysis of duodenal villi and crypts...
December 12, 2017: Journal of Bone and Mineral Metabolism
https://www.readbyqxmd.com/read/29234935/cardiac-and-respiratory-gated-volumetric-murine-ultrasound
#15
Arvin H Soepriatna, Frederick W Damen, Pavlos P Vlachos, Craig J Goergen
Current cardiovascular ultrasound mainly employs planar imaging techniques to assess function and physiology. These techniques rely on geometric assumptions, which are dependent on the imaging plane, susceptible to inter-observer variability, and may be inaccurate when studying complex diseases. Here, we developed a gated volumetric murine ultrasound technique to visualize cardiovascular motion with high spatiotemporal resolution and directly evaluate cardiovascular health. Cardiac and respiratory-gated cine loops, acquired at 1000 frames-per-second from sequential positions, were temporally registered to generate a four-dimensional (4D) dataset...
December 12, 2017: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/29234857/high-frequency-and-wide-range-of-human-kidney-papillary-crystalline-plugs
#16
Léa Huguet, Marine Le Dudal, Marine Livrozet, Dominique Bazin, Vincent Frochot, Joëlle Perez, Jean-Philippe Haymann, Isabelle Brocheriou, Michel Daudon, Emmanuel Letavernier
Most of kidney stones are supposed to originate from Randall's plaque at the tip of the papilla or from papillary tubular plugs. Nevertheless, the frequency and the composition of crystalline plugs remain only partly described. The objective was to assess the frequency, the composition and the topography of papillary plugs in human kidneys. A total of 76 papillae from 25 kidneys removed for cancer and without stones were analysed by immunohistochemistry combined with Yasue staining, field emission-scanning electron microscopy and Fourier transformed infrared micro-spectroscopy...
December 12, 2017: Urolithiasis
https://www.readbyqxmd.com/read/29234186/primary-leiomyosarcoma-of-the-mesentery-a-case-report
#17
Varsha Dalal, Manveen Kaur, Reetika Menia, Fouzia Siraj, Amar Bhatnagar, Usha Agrawal
Mesenteric masses are infrequent lesions ranging from benign cysts to aggressive malignancies and often present a diagnostic and therapeutic challenge. The mesentery is a frequent recipient of metastases from the gastrointestinal tract, pancreas, and biliary cancers. Primary mesenteric tumors are relatively rare, mostly mesenchymal in origin and benign in nature. Examples include gastrointestinal stromal tumors and smooth muscle tumors. We describe a 50-year-old woman, who presented with a lump in the left hypochondrium along with altered bowel habits of 2 years' duration...
September 2017: Iranian Journal of Medical Sciences
https://www.readbyqxmd.com/read/29234087/conformational-switch-regulates-the-dna-cytosine-deaminase-activity-of-human-apobec3b
#18
Ke Shi, Özlem Demir, Michael A Carpenter, Jeff Wagner, Kayo Kurahashi, Reuben S Harris, Rommie E Amaro, Hideki Aihara
The APOBEC3B (A3B) single-stranded DNA (ssDNA) cytosine deaminase has important roles in innate immunity but is also a major endogenous source of mutations in cancer. Previous structural studies showed that the C-terminal catalytic domain of human A3B has a tightly closed active site, and rearrangement of the surrounding loops is required for binding to substrate ssDNA. Here we report structures of the A3B catalytic domain in a new crystal form that show alternative, yet still closed, conformations of active site loops...
December 12, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29233991/structure-of-outer-membrane-protein-g-in-lipid-bilayers
#19
Joren S Retel, Andrew J Nieuwkoop, Matthias Hiller, Victoria A Higman, Emeline Barbet-Massin, Jan Stanek, Loren B Andreas, W Trent Franks, Barth-Jan van Rossum, Kutti R Vinothkumar, Lieselotte Handel, Gregorio Giuseppe de Palma, Benjamin Bardiaux, Guido Pintacuda, Lyndon Emsley, Werner Kühlbrandt, Hartmut Oschkinat
β-barrel proteins mediate nutrient uptake in bacteria and serve vital functions in cell signaling and adhesion. For the 14-strand outer membrane protein G of Escherichia coli, opening and closing is pH-dependent. Different roles of the extracellular loops in this process were proposed, and X-ray and solution NMR studies were divergent. Here, we report the structure of outer membrane protein G investigated in bilayers of E. coli lipid extracts by magic-angle-spinning NMR. In total, 1847 inter-residue 1H-1H and 13C-13C distance restraints, 256 torsion angles, but no hydrogen bond restraints are used to calculate the structure...
December 12, 2017: Nature Communications
https://www.readbyqxmd.com/read/29233981/a-mir-327-fgf10-fgfr2-mediated-autocrine-signaling-mechanism-controls-white-fat-browning
#20
Carina Fischer, Takahiro Seki, Sharon Lim, Masaki Nakamura, Patrik Andersson, Yunlong Yang, Jennifer Honek, Yangang Wang, Yanyan Gao, Fang Chen, Nilesh J Samani, Jun Zhang, Masato Miyake, Seiichi Oyadomari, Akihiro Yasue, Xuri Li, Yun Zhang, Yizhi Liu, Yihai Cao
Understanding the molecular mechanisms regulating beige adipocyte formation may lead to the development of new therapies to combat obesity. Here, we report a miRNA-based autocrine regulatory pathway that controls differentiation of preadipocytes into beige adipocytes. We identify miR-327 as one of the most downregulated miRNAs targeting growth factors in the stromal-vascular fraction (SVF) under conditions that promote white adipose tissue (WAT) browning in mice. Gain- and loss-of-function experiments reveal that miR-327 targets FGF10 to prevent beige adipocyte differentiation...
December 12, 2017: Nature Communications
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