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https://www.readbyqxmd.com/read/29777997/rumen-fluid-fermentation-for-enhancement-of-hydrolysis-and-acidification-of-grass-clipping
#1
Siqi Wang, Guangming Zhang, Panyue Zhang, Xiaowen Ma, Fan Li, Haibo Zhang, Xue Tao, Junpei Ye, Mohammad Nabi
Rumen fluid, formed in rumen of ruminants, includes a complex microbial population of bacteria, protozoa, fungi and archaea, and has high ability to degrade lignocellulosic biomass. In this study, rumen fluid was used to ferment grass clipping for enhancing the hydrolysis and acidification of organic matters. Results showed that strict anaerobic condition, higher grass clipping content and smaller particle size of grass clipping were beneficial to the hydrolysis and acidification of organics. The increase of SCOD and total VFA concentration respectively reached 24...
May 16, 2018: Journal of Environmental Management
https://www.readbyqxmd.com/read/29777704/dietary-mung-bean-protein-reduces-high-fat-diet-induced-weight-gain-by-modulating-host-bile-acid-metabolism-in-a-gut-microbiota-dependent-manner
#2
Akiho Nakatani, Xuan Li, Junki Miyamoto, Miki Igarashi, Hitoshi Watanabe, Asuka Sutou, Keita Watanabe, Takayasu Motoyama, Nobuhiko Tachibana, Mitsutaka Kohno, Hiroshi Inoue, Ikuo Kimura
The 8-globulin-rich mung bean protein (MPI) suppresses hepatic lipogenesis in rodent models and reduces fasting plasma glucose and insulin levels in obese adults. However, its effects on mitigating high fat diet (HFD)-induced obesity and the mechanism underlying these effects remain to be elucidated. Herein, we examined the metabolic phenotype, intestinal bile acid (BA) pool, and gut microbiota of conventionally raised (CONV-R) male C57BL/6 mice and germ-free (GF) mice that were randomized to receive either regular HFD or HFD containing mung bean protein isolate (MPI) instead of the dairy protein present in regular HFD...
May 16, 2018: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29777622/comparative-analysis-of-the-bacterial-community-compositions-of-the-shrimp-intestine-surrounding-water-and-sediment
#3
Dongwei Hou, Zhijian Huang, Shenzheng Zeng, Jian Liu, Shaoping Weng, Jianguo He
AIMS: To reveal the relationship of the bacterial communities in shrimp intestine and surrounding environments. METHODS AND RESULTS: We examined bacterial communities in intestine of pacific white shrimp, Litopenaeus vannamei, the surrounding water and sediment by high-throughput sequencing analysis. Sequences were clustered into operational taxonomic units (OTUs) at 97% similarity levels, which ranged from 4,956 to 5,976 in each sample. All OTUs were affiliated with at least 64 phyla...
May 19, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29776922/core-vs-diet-associated-and-postprandial-bacterial-communities-of-the-rainbow-trout-oncorhynchus-mykiss-midgut-and-feaces
#4
Eleni Mente, Eleni Nikouli, Efthimia Antonopoulou, Samuel A M Martin, Konstantinos Ar Kormas
This study investigated the impact of different dietary ingredients with different protein/lipid sources on the midgut and feaces bacteria communities structure just before feeding and 3h after feeding a single meal on rainbow trout ( Oncorhynchus mykiss ) individuals. Fish were kept in experimental rearing facilities and fed ad libitum two times daily for five weeks. Fish were fed three different commercial diets containing a higher marine fishmeal/fish oil and a lower marine fishmeal/fish oil content. DNA was extracted from midgut and faeces samples for the analysis of their bacterial 16S rRNA gene diversity by targeting the V3-V4 region with 454 pyrosequencing...
May 18, 2018: Biology Open
https://www.readbyqxmd.com/read/29775545/effect-of-bamboo-vinegar-powder-as-an-antibiotic-alternative-on-the-digesta-bacteria-communities-of-finishing-pigs
#5
Huan Qu, Yanjie Huang, Yinghao Shi, Ying Liu, Shenglong Wu, Wenbin Bao
This study investigated the use for bamboo vinegar powder as an antibiotic alternative in the diet of growing-finishing pigs by examining their digestive bacterial communities. Forty-five Duroc × Landrace × Yorkshire growing-finishing pigs were randomly allocated to five diet groups: 0%,0.5%, 1.0%, or 1.5% bamboo vinegar levels and antibiotics. After 37 days, the digesta in duodenum of four pigs from each treatment were analyzed for their bacterial community compositions using 16S rRNA gene sequencing. Addition of 1...
May 18, 2018: Canadian Journal of Microbiology
https://www.readbyqxmd.com/read/29775095/study-of-microbial-diversity-in-plant-microbe-interaction-system-with-oil-sludge-contamination
#6
Monika Dhote, Anil Kumar, Anjana Jajoo, Asha Juwarkar
A 90 days greenhouse experiment was conducted for evaluation of soil microbial diversity in different treatments of rhizospheric and nonrhizospheric oil sludge contaminated soil. Various pot treatments (T1-T5) were as follows: 2% oil sludge contaminated soil was considered as control (T1); augmentation of control with preadapted microbial consortium was T2; addition of Vetiver zizanioide to control was T3; bioaugmentation of control along with V. zizanioide was T4; and bioaugmentation with V. zizanioide and bulking agent was T5...
July 3, 2018: International Journal of Phytoremediation
https://www.readbyqxmd.com/read/29772500/influence-of-zeolite-and-superphosphate-as-additives-on-antibiotic-resistance-genes-and-bacterial-communities-during-factory-scale-chicken-manure-composting
#7
Shuang Peng, Huijie Li, Dan Song, Xiangui Lin, Yiming Wang
Factory-scale chicken manure composting added with zeolite (F), superphosphate (G), or zeolite and ferrous sulfate (FL) simultaneously, were evaluate for their effects on the behaviors of antibiotic resistance genes (ARGs) and bacterial communities. After composting, ARGs in manure decreased by 67.3% in the control, whereas the reductions were 86.5%, 68.6% and 72.2% in F, G and FL, respectively. ARGs encoding ribosomal protection proteins (tetO, tetB(P), and tetM) were reduced to a greater extent than tetG, tetL, sul1 and sul2...
April 30, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29772229/polybacterial-community-analysis-in-human-conjunctiva-through-16s-rrna-gene-libraries
#8
Deepthi KrishnanNair Geetha, Jayasudha Rajagopalaboopathi, Girish Rameshan Nair, Manikandan Palanisamy, Ram Rammohan, Narendran Venkatapathy, Prabagaran Solai Ramatchandirane
The conjunctival sac of healthy human harbours a variety of microorganisms. When the eye is compromised, an occasional inadvertent spread happens to the adjacent tissue, resulting in bacterial ocular infections. Microbiological investigation of the conjunctival swab is one of the broadly used modality to study the aetiological agent of conjunctiva. However, most of the time such methods yield unsatisfactory results. Hence, the present study intends to identify the bacterial community in human conjunctiva of pre-operative subjects through 16S rRNA gene libraries...
May 14, 2018: Experimental Eye Research
https://www.readbyqxmd.com/read/29771989/bacterial-dna-is-present-in-the-fetal-intestine-and-overlaps-with-that-in-the-placenta-in-mice
#9
Keith A Martinez, Joann Romano-Keeler, Joseph P Zackular, Daniel J Moore, Robert M Brucker, Christopher Hooper, Shufang Meng, Naoko Brown, Simon Mallal, Jeff Reese, David M Aronoff, Hakdong Shin, Maria Gloria Dominguez-Bello, Jörn-Hendrik Weitkamp
Bacterial DNA has been reported in the placenta and amniotic fluid by several independent groups of investigators. However, it's taxonomic overlap with fetal and maternal bacterial DNA in different sites has been poorly characterized. Here, we determined the presence of bacterial DNA in the intestines and placentas of fetal mice at gestational day 17 (n = 13). These were compared to newborn intestines (n = 15), maternal sites (mouth, n = 6; vagina, n = 6; colon, n = 7; feces, n = 8), and negative controls to rule out contamination...
2018: PloS One
https://www.readbyqxmd.com/read/29771400/inflammatory-cytokines-il-6-il-10-il-13-tnf-%C3%AE-and-peritoneal-fluid-flora-were-associated-with-infertility-in-patients-with-endometriosis
#10
X-M Wang, Z-Y Ma, N Song
OBJECTIVE: To investigate the correlations of inflammatory factors, interleukin-6 (IL-6), IL-10, IL-13 and tumor necrosis factor-α (TNF-α), and composition of bacterial flora in the peritoneal fluid with infertility in endometriosis patients. PATIENTS AND METHODS: A total of 55 patients diagnosed with endometriosis and infertility in the Gynecology Clinic of our hospital from June 2014 to July 2017 were selected as observation group, and another 30 non-endometriosis and non-infertility patients were enrolled as control group...
May 2018: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/29767727/effects-of-long-term-intake-of-a-yogurt-fermented-with-lactobacillus-delbrueckii-subsp-bulgaricus-2038-and-streptococcus-thermophilus-1131-on-mice
#11
Yuki Usui, Yasumasa Kimura, Takeshi Satoh, Naoki Takemura, Yasuo Ouchi, Hiroko Ohmiya, Kyosuke Kobayashi, Hiromi Suzuki, Satomi Koyama, Satoko Hagiwara, Hirotoshi Tanaka, Seiya Imoto, Gérard Eberl, Yukio Asami, Kosuke Fujimoto, Satoshi Uematsu
The gut is an extremely complicated ecosystem where microorganisms, nutrients and host cells interact vigorously. Although the function of the intestine and its barrier system weakens with age, some probiotics can potentially prevent age-related intestinal dysfunction. Lactobacillus delbrueckii subsp. bulgaricus 2038 and Streptococcus thermophilus 1131, which are the constituents of LB81 yogurt, are representative probiotics. However, it is unclear whether their long-term intake has a beneficial influence on systemic function...
May 15, 2018: International Immunology
https://www.readbyqxmd.com/read/29767159/niacin-alters-the-ruminal-microbial-composition-of-cattle-under-high-concentrate-condition
#12
Dan Luo, Yufei Gao, Youyou Lu, Mingren Qu, Xiaowen Xiong, Lanjiao Xu, Xianghui Zhao, Ke Pan, Kehui Ouyang
To understand the effects of niacin on the ruminal microbial ecology of cattle under high-concentrate diet condition, Illumina MiSeq sequencing technology was used. Three cattle with rumen cannula were used in a 3 × 3 Latin-square design trial. Three diets were fed to these cattle during 3 periods for 3 days, respectively: high-forage diet (HF; forage-to-concentrate ratio = 80:20), high-concentrate diet (HC; forage-to-concentrate ratio = 20:80), and HC supplemented with 800 mg/kg niacin (HCN). Ruminal pH was measured before feeding and every 2 h after initiating feeding...
June 2017: Animal nutrition
https://www.readbyqxmd.com/read/29766722/effects-of-whole-grain-rice-and-wheat-on-composition-of-gut-microbiota-and-short-chain-fatty-acids-in-rats
#13
Fei Han, Yong Wang, Yangyang Han, Jianxin Zhao, Fenli Han, Ge Song, Ping Jiang, Haijiang Miao
Diets rich in whole grain (WG) cereals bring lower disease risks compared with refined grain-based diets. We investigated the effects of polished rice (PR), refined wheat (RW), unpolished rice (UPR), and whole wheat (WW) on short-chain fatty acids (SCFAs) and gut microbiota in ileal, cecal, and colonic digesta of normal rats. Animals fed with UPR and WW diets exhibited higher total SCFA in cecal and colonic digesta compared with those fed with PR and RW diets. Wheat diets contributed higher total SCFA than rice diets...
May 16, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29765642/glucocorticoids-modulate-gastrointestinal-microbiome-in-a-wild-bird
#14
José C Noguera, Manuel Aira, Marcos Pérez-Losada, Jorge Domínguez, Alberto Velando
It has recently been hypothesized that stress exposure (e.g. via glucocorticoid secretion) may dysregulate the bacterial gut microbiome, a crucial 'organ' in animal health. However, whether stress exposure (e.g. via glucocorticoid secretion) affects the bacterial gut microbiome of natural populations is unknown. We have experimentally altered the basal glucocorticoid level (corticosterone implants) in a wild avian species, the yellow-legged gull Larus michahellis , to assess its effects on the gastrointestinal microbiota...
April 2018: Royal Society Open Science
https://www.readbyqxmd.com/read/29765370/pig-farmers-homes-harbor-more-diverse-airborne-bacterial-communities-than-pig-stables-or-suburban-homes
#15
Ditte V Vestergaard, Gitte J Holst, Ioannis Basinas, Grethe Elholm, Vivi Schlünssen, Allan Linneberg, Tina Šantl-Temkiv, Kai Finster, Torben Sigsgaard, Ian P G Marshall
Airborne bacterial communities are subject to conditions ill-suited to microbial activity and growth. In spite of this, air is an important transfer medium for bacteria, with the bacteria in indoor air having potentially major consequences for the health of a building's occupants. A major example is the decreased diversity and altered composition of indoor airborne microbial communities as a proposed explanation for the increasing prevalence of asthma and allergies worldwide. Previous research has shown that living on a farm confers protection against development of asthma and allergies, with airborne bacteria suggested as playing a role in this protective effect...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29765364/rearing-water-treatment-induces-microbial-selection-influencing-the-microbiota-and-pathogen-associated-transcripts-of-cod-gadus-morhua-larvae
#16
Ragnhild I Vestrum, Kari J K Attramadal, Per Winge, Keshuai Li, Yngvar Olsen, Atle M Bones, Olav Vadstein, Ingrid Bakke
We have previously shown that K-selection and microbial stability in the rearing water increases survival and growth of Atlantic cod ( Gadus morhua ) larvae, and that recirculating aquaculture systems (RAS) are compatible with this. Here, we have assessed how water treatment influenced the larval microbiota and host responses at the gene expression level. Cod larvae were reared with two different rearing water systems: a RAS and a flow-through system (FTS). The water microbiota was examined using a 16S rDNA PCR/DGGE strategy...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29763800/potentials-of-using-mixed-culture-bacteria-incorporated-with-sodium-bicarbonate-for-hydrogen-production-from-water-hyacinth
#17
Alaa Wazeri, Mohamed Elsamadony, Sophie Le Roux, Pascal Peu, Ahmed Tawfik
The aim of this study is to assess the potentials of using mixed culture bacteria incorporated with different concentrations of NaHCO3 for hydrogen production from water hyacinth (WH). The lowest hydrogen yield (HY) of 30.4 ± 1.9 mL/gTVS , H2 content (HC) of 19.5 ± 1.5% and hydrogenase enzyme (HE) activity of 0.06 ± 0.01 mgM.Breduced /min were registered for the cultures without supplementation of NaHCO3 . The HY, HC, and HE activity were maximized at levels of 69.2 ± 4.3 mL/gTVS, 58...
May 8, 2018: Bioresource Technology
https://www.readbyqxmd.com/read/29762899/metagenomics-analysis-of-gut-microbiota-in-a-high-fat-diet-induced-obesity-mouse-model-fed-with-epigallocatechin-3-o-3-o-methyl-gallate-egcg3-me
#18
Xin Zhang, Yuhui Chen, Jieyu Zhu, Man Zhang, Chi-Tang Ho, Qingrong Huang, Jinxuan Cao
SCOPE: (-)-Epigallocatechin 3-O-(3-O-methyl) gallate (EGCG3''Me) has been shown to have a modulatory effect on human intestinal microbiota, and the relationship between intestinal flora and obesity has attracted more and more attention recently. Here, we investigated the potential link between EGCG3''Me and gut microbiota composition, as well as the underlying mechanisms of the anti-obesity activity of EGCG3''Me. METHODS AND RESULTS: EGCG3''Me was prepared from oolong tea by column chromatography, and the influence of EGCG3''Me on intestinal microbiota was analysed using a human flora-associated high fat diet (HFD)-induced obesity mouse model by metagenomics...
May 15, 2018: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29762699/cultivable-bacteria-from-pectinatella-magnifica-and-the-surrounding-water-in-south-bohemia-indicate-potential-new-gammaproteobacterial-betaproteobacterial-and-firmicutes-taxa
#19
Hana Salmonová, Jirí Killer, Vera Bunešová, Martina Geigerová, Eva Vlková
Pectinatella magnifica is a freshwater bryozoan, which has become a subject of scientific interest because of its invasive expansion worldwide. To obtain a comprehensive overview of its influence on environments, information on associated bacteria is needed. In this study, cultivable bacteria associated with P. magnifica were investigated. In total, 253 isolates were selected for preliminary identification by MALDI-TOF mass spectrometry and clustered based on REP-PCR profiles. Among these, 169 strains were selected and identified using 16S rRNA gene comparative analyses...
May 14, 2018: FEMS Microbiology Letters
https://www.readbyqxmd.com/read/29760910/the-influence-of-diet-and-environment-on-the-gut-microbial-community-of-field-crickets
#20
Soon Hwee Ng, Michael Stat, Michael Bunce, Leigh W Simmons
The extent to which diet and environment influence gut community membership (presence or absence of taxa) and structure (individual taxon abundance) is the subject of growing interest in microbiome research. Here, we examined the gut bacterial communities of three cricket groups: (1) wild caught field crickets, (2) laboratory-reared crickets fed cat chow, and (3) laboratory-reared crickets fed chemically defined diets. We found that both environment and diet greatly altered the structure of the gut bacterial community...
May 2018: Ecology and Evolution
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