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https://www.readbyqxmd.com/read/29349504/oral-bacterial-colonization-on-dental-implants-restored-with-titanium-or-zirconia-abutments-6-month-follow-up
#1
Alice Ramos de Freitas, Thalisson Saymo de Oliveira Silva, Ricardo Faria Ribeiro, Rubens Ferreira de Albuquerque Junior, Vinícius Pedrazzi, Cássio do Nascimento
OBJECTIVE: This investigation aimed to characterize in a 6-month follow-up the microbial profile of implants restored with either titanium or zirconia abutments at the genus or higher taxonomic levels. METHODS: Twenty healthy individuals indicative for implant-retained single restorations were investigated. Half of participants were restored with titanium and half with zirconia abutments. Biofilm was collected from the implant-related sites after 1, 3, and 6 months of loading...
January 18, 2018: Clinical Oral Investigations
https://www.readbyqxmd.com/read/29345748/kudingcha-and-fuzhuan-brick-tea-prevent-obesity-and-modulate-gut-microbiota-in-high-fat-diet-fed-mice
#2
Guijie Chen, Minhao Xie, Zhuqing Dai, Peng Wan, Hong Ye, Xiaoxiong Zeng, Yi Sun
SCOPE: Kudingcha (KDC) from Ilex kudingcha and Fuzhuan brick tea (FBT) are popular beverages in China, and their preventive and therapeutic roles in metabolic disorders have been reported. However, the relationship between the gut microbiota modulatory effects of KDC and FBT and prevention of obesity is still not clearly understood. METHODS AND RESULTS: KDC and FBT were tested individually for their capacities to prevent obesity and modulate the gut microbiota in high-fat diet (HFD) fed C57BL/6J mice...
January 18, 2018: Molecular Nutrition & Food Research
https://www.readbyqxmd.com/read/29345191/enhanced-sulfide-removal-and-bioelectricity-generation-in-microbial-fuel-cells-with-anodes-modified-by-vertically-oriented-nanosheets
#3
Meng Yang, Yuezhi Zhong, Baogang Zhang, Jiaxin Shi, Xueyang Huang, Yi Xing, Lin Su, Huipeng Liu, Alistair G L Borthwick
Anode materials and structures are of critical importance for microbial fuel cells (MFCs) recovering energy from toxic substrates. Carbon-fiber-felt anodes modified by layers of vertically oriented TiO2 and Fe2O3 nanosheets respectively were applied in present study. Enhanced sulfide removal efficiencies (both over 90%) were obtained after 48 h operation, with maximum power densities improved by 1.53 and 1.36 folds compared with MFCs with raw carbon-fiber-felt anode, respectively. The modified anodes provided more active sites for microbial adhesion with increasing biomass densities...
January 18, 2018: Environmental Technology
https://www.readbyqxmd.com/read/29341193/impact-of-trans-fats-on-heat-shock-protein-expression-and-the-gut-microbiota-profile-of-mice
#4
Gessika Cristina Borges Castro Carvalho, Carolina Soares Moura, Aline Rissetti Roquetto, Daniel Barrera-Arellano, Aureo Tatsumi Yamada, Andrey Dos Santos, Mário José Abdalla Saad, Jaime Amaya-Farfan
Partially hydrogenated oils are known to cause metabolic stress and dyslipidemia. This paper explores a new dimension about the interaction between dietary trans-fats and the defense heat-shock protein (HSP) system, inflammation, and the gut microbiota of mice consuming a hyperlipidic diet containing partially hydrogenated vegetable oil free of animal fat. Five diet groups were installed: control diet, 2 hyperlipidic-partially hydrogenated-oil diets, each containing either casein or whey-protein hydrolysate (WPH) as protein source, and 2 consuming hyperlipidic-unhydrogenated-oil diets containing either WPH or casein as a protein source...
January 17, 2018: Journal of Food Science
https://www.readbyqxmd.com/read/29340714/taxonomical-resolution-and-distribution-of-bacterioplankton-along-the-vertical-gradient-reveals-pronounced-spatiotemporal-patterns-in-contrasted-temperate-freshwater-lakes
#5
J Keshri, A S Pradeep Ram, P A Nana, T Sime-Ngando
We examined the relationship between viruses and co-occurring bacterial communities across spatiotemporal scale in two contrasting freshwater lakes, namely meromictic Lake Pavin and dimictic Lake Aydat (Central France). Next-generation sequencing of 16S rRNA genes suggested distinct patterns in bacterioplankton community composition (BCC) between the lakes over depths and seasons. BCC were generally dominated by members of Actinobacteria, Proteobacteria, and Bacteroidetes covering about 95% of all sequences...
January 16, 2018: Microbial Ecology
https://www.readbyqxmd.com/read/29339620/the-membrane-fouling-mechanisms-of-the-pac-bpac-uf-combined-process-used-to-treat-the-secondary-effluent-from-municipal-wastewater-treatment-plant
#6
Lihua Sun, Ning He, Xi Duan, Bingbing Yang, Cuimin Feng, Yajun Zhang
The combined processes of powdered activated carbon/biological powdered activated carbon- ultrafiltration (PAC/BPAC-UF) were used to treat secondary effluent. In this study, the effect of PAC and BPAC on membrane flux, membrane resistance and the removal of different molecular weight organic compounds were investigated. In addition, the structure characteristics of the microorganisms of the BPAC were analyzed. The results showed that the optimum dosage of PAC and BPAC was 10 mg/L and 40 mg/L respectively. The reversible membrane fouling resistance of BPAC-UF was higher than that of PAC-UF, and the two processes had the least irreversible resistance at the best dosage...
January 2018: Water Science and Technology: a Journal of the International Association on Water Pollution Research
https://www.readbyqxmd.com/read/29338841/the-starch-utilization-system-assembles-around-stationary-starch-binding-proteins
#7
Hannah H Tuson, Matthew H Foley, Nicole M Koropatkin, Julie S Biteen
Bacteroides thetaiotaomicron (Bt) is a prominent member of the human gut microbiota with an extensive capacity for glycan harvest. This bacterium expresses a five-protein complex in the outer membrane, called the starch utilization system (Sus), which binds, degrades, and imports starch into the cell. Sus is a model system for the many glycan-targeting polysaccharide utilization loci found in Bt and other members of the Bacteroidetes phylum. Our previous work has shown that SusG, a lipidated amylase in the outer membrane, explores the entire cell surface but diffuses more slowly as it interacts with starch...
January 12, 2018: Biophysical Journal
https://www.readbyqxmd.com/read/29338683/genomic-repeats-misassembly-and-reannotation-a-case-study-with-long-read-resequencing-of-porphyromonas-gingivalis-reference-strains
#8
Luis Acuña-Amador, Aline Primot, Edouard Cadieu, Alain Roulet, Frédérique Barloy-Hubler
BACKGROUND: Without knowledge of their genomic sequences, it is impossible to make functional models of the bacteria that make up human and animal microbiota. Unfortunately, the vast majority of publicly available genomes are only working drafts, an incompleteness that causes numerous problems and constitutes a major obstacle to genotypic and phenotypic interpretation. In this work, we began with an example from the class Bacteroidia in the phylum Bacteroidetes, which is preponderant among human orodigestive microbiota...
January 16, 2018: BMC Genomics
https://www.readbyqxmd.com/read/29333979/effect-of-anoxic-to-aerobic-duration-ratios-on-nitrogen-removal-and-nitrous-oxide-emission-in-the-multiple-anoxic-aerobic-process
#9
Huoqing Wang, Yuepeng Sun, Guangxue Wu, Yuntao Guan
Characteristics of nitrogen removal and nitrous oxide (N2O) emission in the multiple anoxic/aerobic (AO) process were examined in three sequencing batch reactors (SBRs) with different anoxic durations (50 min, SBRH; 40 min, SBRM; 30 min, SBRL) and a fixed aerobic duration of 30 min. The highest total inorganic nitrogen removal percentage of 85.8% was obtained in SBRH, while a minimum N2O emission factor of 1.9% was obtained in SBRL. During nitrification batch experiments, the N2O emission factor and emission rate were both lower in SBRH than SBRL...
January 15, 2018: Environmental Technology
https://www.readbyqxmd.com/read/29333583/the-microbial-community-of-tardigrades-environmental-influence-and-species-specificity-of-microbiome-structure-and-composition
#10
Matteo Vecchi, Irene L G Newton, Michele Cesari, Lorena Rebecchi, Roberto Guidetti
Symbiotic associations of metazoans with bacteria strongly influence animal biology since bacteria are ubiquitous and virtually no animal is completely free from them. Tardigrades are micrometazoans famous for their ability to undergo ametabolic states (cryptobiosis) but very little information is available on potential microbial associations. We characterized the microbiomes of six limnoterrestrial tardigrade species belonging to several phylogenetic lines in tandem with the microbiomes of their respective substrates...
January 15, 2018: Microbial Ecology
https://www.readbyqxmd.com/read/29330494/characterizing-the-bacterial-microbiota-in-different-gastrointestinal-tract-segments-of-the-bactrian-camel
#11
Jing He, Li Yi, Le Hai, Liang Ming, Wanting Gao, Rimutu Ji
The bacterial community plays important roles in the gastrointestinal tracts (GITs) of animals. However, our understanding of the microbial communities in the GIT of Bactrian camels remains limited. Here, we describe the bacterial communities from eight different GIT segments (rumen, reticulum, abomasum, duodenum, ileum, jejunum, caecum, colon) and faeces determined from 11 Bactrian camels using 16S rRNA gene amplicon sequencing. Twenty-seven bacterial phyla were found in the GIT, with Firmicutes, Verrucomicrobia and Bacteroidetes predominating...
January 12, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29330119/temporal-changes-of-the-bacterial-community-colonizing-wheat-straw-in-the-cow-rumen
#12
Wei Jin, Ying Wang, Yuanfei Li, Yanfen Cheng, Weiyun Zhu
This study used Miseq pyrosequencing and scanning electron microscopy to investigate the temporal changes in the bacterial community tightly attached to wheat straw in the cow rumen. The wheat straw was incubated in the rumens and samples were recovered at various times. The wheat straw degradation exhibited three phases: the first degradation phase occurred within 0.5 h, and the second degradation phase occurred after 6 h, with a stalling phase occurring between 0.5 and 6 h. Scanning electron microscopy revealed the colonization of the microorganisms on the wheat straw over time...
January 9, 2018: Anaerobe
https://www.readbyqxmd.com/read/29328957/sphingolipids-in-host-microbial-interactions
#13
REVIEW
Stacey L Heaver, Elizabeth L Johnson, Ruth E Ley
Sphingolipids, a lipid class characterized by a long-chain amino alcohol backbone, serve vital structural and signaling roles in eukaryotes. Though eukaryotes produce sphingolipids, this capacity is phylogenetically highly restricted in Bacteria. Intriguingly, bacterial species commonly associated in high abundance with eukaryotic hosts include sphingolipid producers, such as the Bacteroidetes in the mammalian gut. To date, a role for bacterial sphingolipids in immune system maturation has been described, but their fate and impact in host physiology and metabolism remain to be elucidated...
January 9, 2018: Current Opinion in Microbiology
https://www.readbyqxmd.com/read/29327435/impact-of-dietary-induced-precocious-gut-maturation-on-cecal-microbiota-and-its-relation-to-the-blood-brain-barrier-during-the-postnatal-period-in-rats
#14
N Marungruang, E Arévalo Sureda, A Lefrançoise, B Weström, M Nyman, O Prykhodko, F Fåk Hållenius
BACKGROUND: Precocious maturation of the gastrointestinal barrier (GIB) in newborn mammals can be induced by dietary provocation, but how this affects the gut microbiota and the gut-brain axis remains unknown. The objective of this study was to investigate effects of induced GIB maturation on gut microbiota composition and blood-brain barrier (BBB) permeability. METHODS: Suckling rats were studied at 72 h after gavage with phytohemagglutinin (PHA) or microbial protease (PT) to induce maturation of GIB...
January 12, 2018: Neurogastroenterology and Motility: the Official Journal of the European Gastrointestinal Motility Society
https://www.readbyqxmd.com/read/29325042/biochemistry-of-complex-glycan-depolymerisation-by-the-human-gut-microbiota
#15
Didier Ndeh, Harry J Gilbert
The human gut microbiota (HGM) makes an important contribution to health and disease. It is a complex microbial community of trillions of microbes with a majority of its members represented within two phyla, the Bacteroidetes and Firmicutes, although it also contains species of Actinobacteria and Proteobacteria. Reflecting its importance, the HGM is sometimes referred to as an 'organ' as it performs functions analogous to systemic tissues within the human host. The major nutrients available to the HGM are host and dietary complex carbohydrates...
January 9, 2018: FEMS Microbiology Reviews
https://www.readbyqxmd.com/read/29322139/effects-of-polysaccharides-from-purple-sweet-potatoes-on-immune-response-and-gut-microbiota-composition-in-normal-and-cyclophosphamide-treated-mice
#16
Chao Tang, Jian Sun, Bo Zhou, Changhai Jin, Jun Liu, Juan Kan, Chunlu Qian, Nianfeng Zhang
In this study, three polysaccharides including water-soluble polysaccharide (WSP), dilute alkali-soluble polysaccharide (DASP) and concentrated alkali-soluble polysaccharide (CASP) were extracted from purple sweet potatoes and then administered to normal and cyclophosphamide (CTX) treated mice by gavage. The results showed that WSP and CASP could restore the spleen index and immune cytokine (IL-2 and IL-6) levels in CTX treated mice, while DASP could enhance the levels of TNF-α, IL-2 and IL-6. As compared to the normal control group, WSP and CASP treatment groups exhibited increased levels of Bacteroidetes, Lachnospiraceae and Oscillospira, but decreased levels of Firmicutes, Alcaligenaceae and Sutterella in normal mice...
January 11, 2018: Food & Function
https://www.readbyqxmd.com/read/29317507/identification-of-euglena-gracilis-%C3%AE-1-3-glucan-phosphorylase-and-establishment-of-a-new-glycoside-hydrolase-gh-family-gh149
#17
Sakonwan Kuhaudomlarp, Nicola J Patron, Bernard Henrissat, Martin Rejzek, Gerhard Saalbach, Robert A Field
Glycoside phosphorylases (EC 2.4.x.x) carry out the reversible phosphorolysis of glucan polymers, producing the corresponding sugar 1-phosphate and a shortened glycan chain. β-1,3-Glucan phosphorylase activities have been reported in the photosynthetic Euglenozoan Euglena gracilis, but the cognate protein sequences have not been identified to date. Continuing our efforts to understand the glycobiology of E. gracilis, we identified a candidate phosphorylase sequence, designated EgP1, by proteomic analysis of an enriched cellular protein lysate...
January 9, 2018: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/29314490/a-mixture-of-lactobacillus-species-isolated-from-traditional-fermented-foods-promote-recovery-from-antibiotic-induced-intestinal-disruption-in-mice
#18
Ying Shi, Xi Zhao, Zhao Jianxin, Zhang Hao, Qixiao Zhai, Arjan Narbad, Wei Chen
AIMS: This study evaluated the antibiotic-induced changes in microbial ecology, intestinal dysbiosis and low-grade inflammation; and the combined effect of four different Lactobacillus species on recovery of microbiota composition and improvement of gut barrier function in mice. METHODS AND RESULTS: Administration of the antibiotic ampicillin for 2 weeks decreased microbial community diversity, induced caecum tumefaction and increased gut permeability in mice. Application of a probiotic cocktail of four Lactobacillus species (JUP-Y4) modulated the microbiota community structure and promoted the abundance of potentially beneficial bacteria such as Akkermansia...
January 3, 2018: Journal of Applied Microbiology
https://www.readbyqxmd.com/read/29313548/lactobacillus-plantarum-ccfm10-alleviating-oxidative-stress-and-restoring-the-gut-microbiota-in-d-galactose-induced-aging-mice
#19
Jichun Zhao, Fengwei Tian, Shuang Yan, Qixiao Zhai, Hao Zhang, Wei Chen
Oxidative stress and its correlation with degenerative diseases have been attracting wide attention. In the present study, Lactobacillus plantarum (L. plantarum) CCFM10 and RS15-3 were examined for their abilities to resist oxidative stress in d-galactose (d-gal)-exposed mice. Both strains significantly reversed the changes of hepatic total antioxidant capacity, catalase activity and glutathione content induced by d-gal. The antioxidative abilities of CCFM10 were higher than those of RS15-3. Moreover, the composition of the mice microbiota was changed by d-gal injection, which was characterized by the up-regulated ratio of Firmicutes/Bacteroidetes...
January 9, 2018: Food & Function
https://www.readbyqxmd.com/read/29311620/dietary-soybean-meal-affects-intestinal-homoeostasis-by-altering-the-microbiota-morphology-and-inflammatory-cytokine-gene-expression-in-northern-snakehead
#20
Shuyan Miao, Chenze Zhao, Jinyu Zhu, Juntao Hu, Xiaojing Dong, Longsheng Sun
A 63-day feeding trial was conducted in northern snakehead to observe the effects of a dietary soybean meal substitution on the microbiota community, morphology and inflammatory cytokine gene expression in the intestine. Four isonitrogenous and isoenergetic diets containing increasing levels of soybean meal were used to replace 0%, 25%, 50% and 75% of the defatted fishmeal (diets are referred to G1, G2, G3 and G4, respectively). Different dietary soybean meal substitutions significantly affected the intestinal microbiota composition...
January 8, 2018: Scientific Reports
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