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https://www.readbyqxmd.com/read/29318690/lactose-repressor-hinge-domain-independently-binds-dna
#1
Joseph S Xu, Madeleine N Hewitt, Jaskeerat S Gulati, Matthew A Cruz, Hongli Zhan, Shirley Liu, Kathleen S Matthews
The short 8-10 amino acid "hinge" sequence in lactose repressor (LacI), present in other LacI/GalR family members, links DNA and inducer-binding domains. Structural studies of full-length or truncated LacI-operator DNA complexes demonstrate insertion of the dimeric helical "hinge" structure at the center of the operator sequence. This association bends the DNA ∼40° and aligns flanking semi-symmetric DNA sites for optimal contact by the N-terminal helix-turn helix (HtH) sequences within each dimer. In contrast, the hinge region remains unfolded when bound to nonspecific DNA sequences...
January 10, 2018: Protein Science: a Publication of the Protein Society
https://www.readbyqxmd.com/read/29248914/whole-genome-analysis-of-an-extensive-drug-resistant-acinetobacter-baumannii-st195-isolate-from-a-recipient-after-dcd-renal-transplantation-in-china
#2
Hong Jiang, Luxi Cao, Qixia Shen, Lihui Qu, Yan Jiang, Tingting Qu, Jian Zhang, Yingying Lu, Bingjue Li, Chaohong Zhu, Guangjun Liu, Rending Wang, Miao Chen, Yucheng Wang, Yanfei Wang, Shi Feng, Junwen Wang, Yunsong Yu, Jianyong Wu, Jianghua Chen
BACKGROUND/AIMS: Infection with Acinetobacter baumannii was emerging as one of the leading causes of mortality after donation after cardiac death transpalantion. METHODS: We reported a case of a recipient who underwent DCD renal transplantation and later got infected by A.baumannii. Etests were done to verify the susceptibility test results in clinic. Whole-genome analysis was applied to investigate the resistant mechanism at gene level. RESULTS: The pathogen was isolated from his draining liquid the day after the surgery, and susceptibility test reavealed that it was sensitive to tigecycline...
December 14, 2017: Kidney & Blood Pressure Research
https://www.readbyqxmd.com/read/29239168/variation-in-lov-photoreceptor-activation-dynamics-probed-by-time-resolved-infrared-spectroscopy
#3
James N Iuliano, Agnieszka A Gil, Sergey P Laptenok, Christopher R Hall, Jinnette Tolentino Collado, Andras Lukacs, Safaa A Hag Ahmed, Jenna Abyad, Taraneh Daryaee, Gregory M Greetham, Igor V Sazanovich, Boris Illarionov, Adelbert Bacher, Markus Fischer, Michael Towrie, Jarrod B French, Stephen R Meech, Peter J Tonge
The light, oxygen, voltage (LOV) domain proteins are blue light photoreceptors that utilize a non-covalently bound flavin mononucleotide (FMN) cofactor as the chromophore. The modular nature of these proteins has led to their wide adoption in the emerging fields of optogenetics and optobiology, where the LOV domain has been fused to a variety of output domains leading to novel light-controlled applications. In the present work, we extend our studies of the sub-picosecond to several hundred microsecond transient infrared spectroscopy of the isolated LOV domain AsLOV2 to three full-length photoreceptors in which the LOV domain is fused to an output domain: the LOV-STAS protein, YtvA, the LOV-HTH transcription factor, EL222, and the LOV-histidine kinase, LovK...
December 14, 2017: Biochemistry
https://www.readbyqxmd.com/read/29141951/selector-genes-display-tumor-cooperation-and-inhibition-in-drosophila-epithelium-in-a-developmental-context-dependent-manner
#4
Ram Prakash Gupta, Anjali Bajpai, Pradip Sinha
During animal development, selector genes determine identities of body segments and those of individual organs. Selector genes are also misexpressed in cancers, although their contributions to tumor progression per se remain poorly understood. Using a model of cooperative tumorigenesis, we show that gain of selector genes results in tumor cooperation, but in only select developmental domains of the wing, haltere and eye-antennal imaginal discs of Drosophila larva. Thus, the field selector, Eyeless (Ey), and the segment selector, Ultrabithorax (Ubx), readily cooperate to bring about neoplastic transformation of cells displaying somatic loss of the tumor suppressor, Lgl, but in only those developmental domains that express the homeo-box protein, Homothorax (Hth), and/or the Zinc-finger protein, Teashirt (Tsh)...
November 15, 2017: Biology Open
https://www.readbyqxmd.com/read/29095143/nuclear-microenvironments-modulate-transcription-from-low-affinity-enhancers
#5
Albert Tsai, Anand K Muthusamy, Mariana Rp Alves, Luke D Lavis, Robert H Singer, David L Stern, Justin Crocker
Transcription factors bind low-affinity DNA sequences for only short durations. It is not clear how brief, low-affinity interactions can drive efficient transcription. Here, we report that the transcription factor Ultrabithorax (Ubx) utilizes low-affinity binding sites in the Drosophila melanogaster shavenbaby (svb) locus and related enhancers in nuclear microenvironments of high Ubx concentrations. Related enhancers colocalize to the same microenvironments independently of their chromosomal location, suggesting that microenvironments are highly differentiated transcription domains...
November 2, 2017: ELife
https://www.readbyqxmd.com/read/29029697/the-identification-of-transcriptional-regulation-related-gene-of-laccase-poxc-through-yeast-one-hybrid-screening-from-pleurotus-ostreatus
#6
Yuancheng Qi, Chao Liu, Xiankai Sun, Liyou Qiu, Jinwen Shen
Pleurotus ostreatus laccase gene poxc is transcriptionally induced by copper, and several putative MREs have been found and confirmed in its promoter region. However, the related transcription factors mediating copper response via MREs have not been reported. To isolate MRE binding proteins, we carried out yeast one-hybrid screens. Reporter genes containing two trimers of the cMRE2 and cMRE3 element were prepared and introduced into a yeast strain. The yeast was transformed with library cDNA that represents RNA isolated from CuSO4-treated fungi of P...
November 2017: Fungal Biology
https://www.readbyqxmd.com/read/28972158/ligand-dependent-corepressor-lcor-represses-the-transcription-factor-c-ebp%C3%AE-during-early-adipocyte-differentiation
#7
Hongchao Cao, Shengjie Zhang, Shifang Shan, Chao Sun, Yan Li, Hui Wang, Shuxian Yu, Yi Liu, Feifan Guo, Qiwei Zhai, Yu-Cheng Wang, Jingjing Jiang, Hui Wang, Jun Yan, Wei Liu, Hao Ying
Nuclear receptors (NRs) regulate gene transcription by recruiting coregulators, involved in chromatin remodeling and assembly of the basal transcription machinery. The NR-associated protein ligand-dependent corepressor (LCoR) has previously been shown to suppress hepatic lipogenesis by decreasing the binding of steroid receptor coactivators to thyroid hormone receptor. However, the role of LCoR in adipogenesis has not been established. Here, we show that LCoR expression is reduced in the early stage of adipogenesis in vitro...
September 26, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28926338/melk-is-not-necessary-for-the-proliferation-of-basal-like-breast-cancer-cells
#8
Hai-Tsang Huang, Hyuk-Soo Seo, Tinghu Zhang, Yubao Wang, Baishan Jiang, Qing Li, Dennis L Buckley, Behnam Nabet, Justin M Roberts, Joshiawa Paulk, Shiva Dastjerdi, Georg E Winter, Hilary McLauchlan, Jennifer Moran, James E Bradner, Michael J Eck, Sirano Dhe-Paganon, Jean J Zhao, Nathanael S Gray
Thorough preclinical target validation is essential for the success of drug discovery efforts. In this study, we combined chemical and genetic perturbants, including the development of a novel selective maternal embryonic leucine zipper kinase (MELK) inhibitor HTH-01-091, CRISPR/Cas9-mediated MELK knockout, a novel chemical-induced protein degradation strategy, RNA interference and CRISPR interference to validate MELK as a therapeutic target in basal-like breast cancers (BBC). In common culture conditions, we found that small molecule inhibition, genetic deletion, or acute depletion of MELK did not significantly affect cellular growth...
September 19, 2017: ELife
https://www.readbyqxmd.com/read/28838125/hothead-like-hth1-is-involved-in-anther-cutin-biosynthesis-and-is-required-for-pollen-fertility-in-rice
#9
Ya Xu, Shasha Liu, Yaqin Liu, Sheng Ling, Caisheng Chen, Jialing Yao
The cuticle covering the outer surface of anthers is essential for male reproductive development in plants. However, the mechanism underlying the synthesis of these lipidic polymers remains unclear. HOTHEAD (HTH) in Arabidopsis thaliana is a presumptive glucose-methanol-choline (GMC) oxidoreductase involved in the biosynthesis of long-chain α-,ω-dicarboxylic fatty acids. In this study, we characterized the function of an anther-specific gene HTH1 in rice. HTH1 contains a conserved GMC oxidoreductase-like domain, and the sequence of HTH1 was highly similar to that of HTH in A...
July 1, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28794035/crystal-structure-of-the-dna-binding-domain-of-human-herpesvirus-6a-immediate-early-protein-2
#10
Mitsuhiro Nishimura, Junjie Wang, Aika Wakata, Kento Sakamoto, Yasuko Mori
Immediate early proteins of human herpesvirus 6A (HHV-6A) are expressed at the outset of lytic infection and thereby regulate viral gene expression. Immediate early protein 2 (IE2) of HHV-6A is a transactivator that drives a variety of promoters. The C-terminal region of HHV-6A IE2 is shared among IE2 homologs in betaherpesviruses and is involved in dimerization, DNA binding, and transcription factor binding. In this study, the structure of the IE2 C-terminal domain (IE2-CTD) was determined by X-ray crystallography at a resolution of 2...
November 1, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28764892/expression-and-function-of-spineless-orthologs-correlate-with-distal-deutocerebral-appendage-morphology-across-arthropoda
#11
Emily V W Setton, Logan E March, Erik D Nolan, Tamsin E Jones, Holly Cho, Ward C Wheeler, Cassandra G Extavour, Prashant P Sharma
The deutocerebral (second) head segment is putatively homologous across Arthropoda, in spite of remarkable disparity of form and function of deutocerebral appendages. In Mandibulata this segment bears a pair of sensory antennae, whereas in Chelicerata the same segment bears a pair of feeding appendages called chelicerae. Part of the evidence for the homology of deutocerebral appendages is the conserved function of homothorax (hth), which has been shown to specify antennal or cheliceral fate in the absence of Hox signaling, in both mandibulate and chelicerate exemplars...
October 1, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28755804/a-new-index-to-simplify-the-screening-of-hypertension-in-overweight-or-obese-youth
#12
MULTICENTER STUDY
P Di Bonito, G Valerio, L Pacifico, C Chiesa, C Invitti, A Morandi, C Maffeis, M R Licenziati, M Manco, E Miraglia Del Giudice, M G Baroni, S Loche, G Tornese, M Tomat, G de Simone
BACKGROUND AND AIMS: Hypertension (HTH) is a frequent complication in pediatric obesity. To simplify the screening of HTH in overweight/obese (Ow/Ob) youth, we compared the performance of a new index (High Blood Pressure index, HBPi) with respect to the standard criteria of the IV Report [systolic BP (SBP) and/or diastolic BP (DBP) ≥95th percentile for age, gender and height]. We also compared the performance of HBPi with other simplified indices such as the BP/height ratio and the absolute height-specific BP thresholds...
September 2017: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
https://www.readbyqxmd.com/read/28744295/antarctic-moss-multiprotein-bridging-factor-1c-overexpression-in-arabidopsis-resulted-in-enhanced-tolerance-to-salt-stress
#13
Hemasundar Alavilli, Hyoungseok Lee, Mira Park, Byeong-Ha Lee
Polytrichastrum alpinum is one of the moss species that survives extreme conditions in the Antarctic. In order to explore the functional benefits of moss genetic resources, P. alpinum multiprotein-bridging factor 1c gene (PaMBF1c) was isolated and characterized. The deduced amino acid sequence of PaMBF1c comprises of a multiprotein-bridging factor (MBF1) domain and a helix-turn-helix (HTH) domain. PaMBF1c expression was induced by different abiotic stresses in P. alpinum, implying its roles in stress responses...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28709396/rapid-diversification-of-homothorax-expression-patterns-after-gene-duplication-in-spiders
#14
Natascha Turetzek, Sara Khadjeh, Christoph Schomburg, Nikola-Michael Prpic
BACKGROUND: Gene duplications provide genetic material for the evolution of new morphological and physiological features. One copy can preserve the original gene functions while the second copy may evolve new functions (neofunctionalisation). Gene duplications may thus provide new genes involved in evolutionary novelties. RESULTS: We have studied the duplicated homeobox gene homothorax (hth) in the spider species Parasteatoda tepidariorum and Pholcus phalangioides and have compared these data with previously published data from additional spider species...
July 14, 2017: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/28695853/crystal-structure-of-the-n-terminal-domain-of-vqsr-from-pseudomonas-aeruginosa-at-2-1%C3%A2-%C3%A3-resolution
#15
Qing He, Kang Wang, Tiantian Su, Feng Wang, Lichuan Gu, Sujuan Xu
VqsR is a quorum-sensing (QS) transcriptional regulator which controls QS systems (las, rhl and pqs) by directly downregulating the expression of qscR in Pseudomonas aeruginosa. As a member of the LuxR family of proteins, VqsR shares the common motif of a helix-turn-helix (HTH)-type DNA-binding domain at the C-terminus, while the function of its N-terminal domain remains obscure. Here, the crystal structure of the N-terminal domain of VqsR (VqsR-N; residues 1-193) was determined at a resolution of 2.1 Å...
July 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28687780/nuclear-receptors-connect-progenitor-transcription-factors-to-cell-cycle-control
#16
Marta Neto, Marina Naval-Sánchez, Delphine Potier, Paulo S Pereira, Dirk Geerts, Stein Aerts, Fernando Casares
The specification and growth of organs is controlled simultaneously by networks of transcription factors. While the connection between these transcription factors with fate determinants is increasingly clear, how they establish the link with the cell cycle is far less understood. Here we investigate this link in the developing Drosophila eye, where two transcription factors, the MEIS1 homologue hth and the Zn-finger tsh, synergize to stimulate the proliferation of naïve eye progenitors. Experiments combining transcriptomics, open-chromatin profiling, motif analysis and functional assays indicate that these progenitor transcription factors exert a global regulation of the proliferation program...
July 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28663093/heat-stress-induced-neuroinflammation-and-aberration-in-monoamine-levels-in-hypothalamus-are-associated-with-temperature-dysregulation
#17
Nishant Ranjan Chauhan, Medha Kapoor, Laxmi Prabha Singh, Rajinder Kumar Gupta, Ramesh Chand Meena, Rajkumar Tulsawani, Sarita Nanda, Shashi Bala Singh
Heat Stress (HS) induces diverse pathophysiological changes, which include brain ischemia, oxidative stress and neuronal damage. The present study was undertaken with the objective to ascertain whether neuroinflammation in Hypothalamus (HTH) caused under HS affects monoamine levels and hence, its physiological role in thermoregulation. Rats were exposed to HS in a heat simulation environmental chamber (Ambient temperature, Ta=45±0.5°C and Relative Humidity, RH=30±10%) with real-time measurement of core temperature (Tc) and skin temperature (Ts)...
September 1, 2017: Neuroscience
https://www.readbyqxmd.com/read/28656052/effect-of-huayu-tongluo-herbs-on-reduction-of-proteinuria-via-inhibition-of-wnt-%C3%AE-catenin-signaling-pathway-in-diabetic-rats
#18
Lu Bai, Beibei Huo, Zhiqiang Chen, Qian Guo, Jing Xu, Jing Fang, Jianghua Zhang, Fenfang Zhang
The study investigated the expression of Wnt/β-catenin pathway in diabetic rats and the intervention effect of Huayu Tongluo herbs (HTH). Ten rats were randomly selected as control group and the remaining rats were established as diabetic models. The diabetic rats were randomly divided into model group and HTH treatment group. The intervention was intragastric administration in all rats for 20 weeks. At the end of every 4 weeks, fasting blood glucose and 24 h urinary total protein quantitatively were measured...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28558642/hth-xre-toxin-antitoxin-loci-and-many-unevaluated-toxin-antitoxin-loci-in-pseudomonas-aeruginosa-b136-33-bioinformatics-analysis
#19
Mina Mahmoodi, Mahsa Jalili, Zahra Chavak, Nasrin Valizadeh, Nourkhoda Sadeghifard, Iraj Pakzad, Marayam Mohammadian, Mehrnoush Safikhanimoslem, Abbas Maleki, Sobhan Ghafourian
Pseudomonas aeruginosa is responsible for many infections and also many antibiotic resistance genes existed in this bacterium. Some focused to toxin antitoxin systems demonstrated these systems as regulon of this properties of P. aeruginosa. Therefore, in an analysis we tried to evaluate the potent TA loci and suggest some of them for experimental analysis. For this reasons, RASTA databases were subjected and our results demonstrated hth-xre as dominant known and many unknown TA loci. Despite of distribution of TA loci in all scores but obtained results of hth-xre TA loci suggested these unknown TA loci may be helpful for further both bioinformatics and experimental analysis...
May 30, 2017: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/28483053/gm1-mmp-is-involved-in-growth-and-development-of-leaf-and-seed-and-enhances-tolerance-to-high-temperature-and-humidity-stress-in-transgenic-arabidopsis
#20
Sushuang Liu, Yanmin Liu, Yanhong Jia, Jiaping Wei, Shuang Wang, Xiaolin Liu, Yali Zhou, Yajing Zhu, Weihong Gu, Hao Ma
Matrix metalloproteinases (MMPs) are a family of zinc- and calcium-dependent endopeptidases. Gm1-MMP was found to play an important role in soybean tissue remodeling during leaf expansion. In this study, Gm1-MMP was isolated and characterized. Its encoding protein had a relatively low phylogenetic relationship with the MMPs in other plant species. Subcellular localization indicated that Gm1-MMP was a plasma membrane protein. Gm1-MMP showed higher expression levels in mature leaves, old leaves, pods, and mature seeds, as well as was involved in the development of soybean seed...
June 2017: Plant Science: An International Journal of Experimental Plant Biology
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