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https://www.readbyqxmd.com/read/28926338/melk-is-not-necessary-for-the-proliferation-of-basal-like-breast-cancer-cells
#1
Hai-Tsang Huang, Hyuk-Soo Seo, Tinghu Zhang, Yubao Wang, Baishan Jiang, Qing Li, Dennis L Buckley, Behnam Nabet, Justin M Roberts, Joshiawa Paulk, Shiva Dastjerdi, Georg E Winter, Hilary McLauchlan, Jennifer Moran, James E Bradner, Michael J Eck, Sirano Dhe-Paganon, Jean J Zhao, Nathanael S Gray
Thorough preclinical target validation is essential for the success of drug discovery efforts. In this study, we combined chemical and genetic perturbants, including the development of a novel selective maternal embryonic leucine zipper kinase (MELK) inhibitor HTH-01-091, CRISPR/Cas9-mediated MELK knockout, a novel chemical-induced protein degradation strategy, RNA interference and CRISPR interference to validate MELK as a therapeutic target in basal-like breast cancers (BBC). In common culture conditions, we found that small molecule inhibition, genetic deletion, or acute depletion of MELK did not significantly affect cellular growth...
September 19, 2017: ELife
https://www.readbyqxmd.com/read/28838125/hothead-like-hth1-is-involved-in-anther-cutin-biosynthesis-and-is-required-for-pollen-fertility-in-rice
#2
Ya Xu, Shasha Liu, Yaqin Liu, Sheng Ling, Caisheng Chen, Jialing Yao
The cuticle covering the outer surface of anthers is essential for male reproductive development in plants. However, the mechanism underlying the synthesis of these lipidic polymers remains unclear. HOTHEAD (HTH) in Arabidopsis thaliana is a presumptive glucose-methanol-choline (GMC) oxidoreductase involved in the biosynthesis of long-chain α-,ω-dicarboxylic fatty acids. In this study, we characterized the function of an anther-specific gene HTH1 in rice. HTH1 contains a conserved GMC oxidoreductase-like domain, and the sequence of HTH1 was highly similar to that of HTH in A...
July 1, 2017: Plant & Cell Physiology
https://www.readbyqxmd.com/read/28794035/crystal-structure-of-the-dna-binding-domain-of-human-herpesvirus-6a-immediate-early-protein-2
#3
Mitsuhiro Nishimura, Junjie Wang, Aika Wakata, Kento Sakamoto, Yasuko Mori
Immediate early proteins of human herpesvirus 6A (HHV-6A) are expressed at the outset of lytic infection, and thereby regulate viral gene expression. Immediate early protein 2 (IE2) of HHV-6A is a trans-activator that drives a variety of promoters. The C-terminal region of HHV-6A IE2 is shared among IE2 homologs in beta-herpesviruses, and is involved in dimerization, DNA binding, and transcription factor binding. In this study, the structure of the C-terminal domain (IE2-CTD) was determined by X-ray crystallography at a resolution of 2...
August 9, 2017: Journal of Virology
https://www.readbyqxmd.com/read/28764892/expression-and-function-of-spineless-orthologs-correlate-with-distal-deutocerebral-appendage-morphology-across-arthropoda
#4
Emily V W Setton, Logan E March, Erik D Nolan, Tamsin E Jones, Holly Cho, Ward C Wheeler, Cassandra G Extavour, Prashant P Sharma
The deutocerebral (second) head segment is putatively homologous across Arthropoda, in spite of remarkable disparity of form and function of deutocerebral appendages. In Mandibulata this segment bears a pair of sensory antennae, whereas in Chelicerata the same segment bears a pair of feeding appendages called chelicerae. Part of the evidence for the homology of deutocerebral appendages is the conserved function of homothorax (hth), which has been shown to specify antennal or cheliceral fate in the absence of Hox signaling, in both mandibulate and chelicerate exemplars...
July 29, 2017: Developmental Biology
https://www.readbyqxmd.com/read/28755804/a-new-index-to-simplify-the-screening-of-hypertension-in-overweight-or-obese-youth
#5
MULTICENTER STUDY
P Di Bonito, G Valerio, L Pacifico, C Chiesa, C Invitti, A Morandi, C Maffeis, M R Licenziati, M Manco, E Miraglia Del Giudice, M G Baroni, S Loche, G Tornese, M Tomat, G de Simone
BACKGROUND AND AIMS: Hypertension (HTH) is a frequent complication in pediatric obesity. To simplify the screening of HTH in overweight/obese (Ow/Ob) youth, we compared the performance of a new index (High Blood Pressure index, HBPi) with respect to the standard criteria of the IV Report [systolic BP (SBP) and/or diastolic BP (DBP) ≥95th percentile for age, gender and height]. We also compared the performance of HBPi with other simplified indices such as the BP/height ratio and the absolute height-specific BP thresholds...
September 2017: Nutrition, Metabolism, and Cardiovascular Diseases: NMCD
https://www.readbyqxmd.com/read/28744295/antarctic-moss-multiprotein-bridging-factor-1c-overexpression-in-arabidopsis-resulted-in-enhanced-tolerance-to-salt-stress
#6
Hemasundar Alavilli, Hyoungseok Lee, Mira Park, Byeong-Ha Lee
Polytrichastrum alpinum is one of the moss species that survives extreme conditions in the Antarctic. In order to explore the functional benefits of moss genetic resources, P. alpinum multiprotein-bridging factor 1c gene (PaMBF1c) was isolated and characterized. The deduced amino acid sequence of PaMBF1c comprises of a multiprotein-bridging factor (MBF1) domain and a helix-turn-helix (HTH) domain. PaMBF1c expression was induced by different abiotic stresses in P. alpinum, implying its roles in stress responses...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28709396/rapid-diversification-of-homothorax-expression-patterns-after-gene-duplication-in-spiders
#7
Natascha Turetzek, Sara Khadjeh, Christoph Schomburg, Nikola-Michael Prpic
BACKGROUND: Gene duplications provide genetic material for the evolution of new morphological and physiological features. One copy can preserve the original gene functions while the second copy may evolve new functions (neofunctionalisation). Gene duplications may thus provide new genes involved in evolutionary novelties. RESULTS: We have studied the duplicated homeobox gene homothorax (hth) in the spider species Parasteatoda tepidariorum and Pholcus phalangioides and have compared these data with previously published data from additional spider species...
July 14, 2017: BMC Evolutionary Biology
https://www.readbyqxmd.com/read/28695853/crystal-structure-of-the-n-terminal-domain-of-vqsr-from-pseudomonas-aeruginosa-at-2-1%C3%A2-%C3%A3-resolution
#8
Qing He, Kang Wang, Tiantian Su, Feng Wang, Lichuan Gu, Sujuan Xu
VqsR is a quorum-sensing (QS) transcriptional regulator which controls QS systems (las, rhl and pqs) by directly downregulating the expression of qscR in Pseudomonas aeruginosa. As a member of the LuxR family of proteins, VqsR shares the common motif of a helix-turn-helix (HTH)-type DNA-binding domain at the C-terminus, while the function of its N-terminal domain remains obscure. Here, the crystal structure of the N-terminal domain of VqsR (VqsR-N; residues 1-193) was determined at a resolution of 2.1 Å...
July 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28687780/nuclear-receptors-connect-progenitor-transcription-factors-to-cell-cycle-control
#9
Marta Neto, Marina Naval-Sánchez, Delphine Potier, Paulo S Pereira, Dirk Geerts, Stein Aerts, Fernando Casares
The specification and growth of organs is controlled simultaneously by networks of transcription factors. While the connection between these transcription factors with fate determinants is increasingly clear, how they establish the link with the cell cycle is far less understood. Here we investigate this link in the developing Drosophila eye, where two transcription factors, the MEIS1 homologue hth and the Zn-finger tsh, synergize to stimulate the proliferation of naïve eye progenitors. Experiments combining transcriptomics, open-chromatin profiling, motif analysis and functional assays indicate that these progenitor transcription factors exert a global regulation of the proliferation program...
July 7, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28663093/heat-stress-induced-neuroinflammation-and-aberration-in-monoamine-levels-in-hypothalamus-are-associated-with-temperature-dysregulation
#10
Nishant Ranjan Chauhan, Medha Kapoor, Laxmi Prabha Singh, Rajinder Kumar Gupta, Ramesh Chand Meena, Rajkumar Tulsawani, Sarita Nanda, Shashi Bala Singh
Heat Stress (HS) induces diverse pathophysiological changes, which include brain ischemia, oxidative stress and neuronal damage. The present study was undertaken with the objective to ascertain whether neuroinflammation in Hypothalamus (HTH) caused under HS affects monoamine levels and hence, its physiological role in thermoregulation. Rats were exposed to HS in a heat simulation environmental chamber (Ambient temperature, Ta=45±0.5°C and Relative Humidity, RH=30±10%) with real-time measurement of core temperature (Tc) and skin temperature (Ts)...
September 1, 2017: Neuroscience
https://www.readbyqxmd.com/read/28656052/effect-of-huayu-tongluo-herbs-on-reduction-of-proteinuria-via-inhibition-of-wnt-%C3%AE-catenin-signaling-pathway-in-diabetic-rats
#11
Lu Bai, Beibei Huo, Zhiqiang Chen, Qian Guo, Jing Xu, Jing Fang, Jianghua Zhang, Fenfang Zhang
The study investigated the expression of Wnt/β-catenin pathway in diabetic rats and the intervention effect of Huayu Tongluo herbs (HTH). Ten rats were randomly selected as control group and the remaining rats were established as diabetic models. The diabetic rats were randomly divided into model group and HTH treatment group. The intervention was intragastric administration in all rats for 20 weeks. At the end of every 4 weeks, fasting blood glucose and 24 h urinary total protein quantitatively were measured...
2017: Evidence-based Complementary and Alternative Medicine: ECAM
https://www.readbyqxmd.com/read/28558642/the-novel-strategy-in-combat-with-breast-cancer-ex-vivo-evalu-ation-of-traditionally-used-berberis-vulgaris
#12
Mina Mahmoodi, Mahsa Jalili, Zahra Chavak, Nasrin Valizadeh, Nourkhoda Sadeghifard, Iraj Pakzad, Marayam Mohammadian, Mehrnoush Safikhanimoslem, Abbas Maleki, Sobhan Ghafourian
Pseudomonas aeruginosa is responsible for many infections and also many antibiotic resistance genes existed in this bacterium. Some focused to toxin antitoxin systems demonstrated these systems as regulon of this properties of P. aeruginosa. Therefore, in an analysis we tried to evaluate the potent TA loci and suggest some of them for experimental analysis. For this reasons, RASTA databases were subjected and our results demonstrated hth-xre as dominant known and many unknown TA loci. Despite of distribution of TA loci in all scores but obtained results of hth-xre TA loci suggested these unknown TA loci may be helpful for further both bioinformatics and experimental analysis...
May 30, 2017: Infectious Disorders Drug Targets
https://www.readbyqxmd.com/read/28483053/gm1-mmp-is-involved-in-growth-and-development-of-leaf-and-seed-and-enhances-tolerance-to-high-temperature-and-humidity-stress-in-transgenic-arabidopsis
#13
Sushuang Liu, Yanmin Liu, Yanhong Jia, Jiaping Wei, Shuang Wang, Xiaolin Liu, Yali Zhou, Yajing Zhu, Weihong Gu, Hao Ma
Matrix metalloproteinases (MMPs) are a family of zinc- and calcium-dependent endopeptidases. Gm1-MMP was found to play an important role in soybean tissue remodeling during leaf expansion. In this study, Gm1-MMP was isolated and characterized. Its encoding protein had a relatively low phylogenetic relationship with the MMPs in other plant species. Subcellular localization indicated that Gm1-MMP was a plasma membrane protein. Gm1-MMP showed higher expression levels in mature leaves, old leaves, pods, and mature seeds, as well as was involved in the development of soybean seed...
June 2017: Plant Science: An International Journal of Experimental Plant Biology
https://www.readbyqxmd.com/read/28414308/complex-regulation-of-lcor-signaling-in-breast-cancer-cells
#14
S Jalaguier, C Teyssier, T Nait Achour, A Lucas, S Bonnet, C Rodriguez, N Elarouci, M Lapierre, V Cavaillès
Ligand-dependent corepressor (LCoR) is a transcriptional repressor of ligand-activated estrogen receptors (ERs) and other transcription factors that acts both by recruiting histone deacetylases and C-terminal binding proteins. Here, we first studied LCOR gene expression in breast cancer cell lines and tissues. We detected two mRNAs variants, LCoR and LCoR2 (which encodes a truncated LCoR protein). Their expression was highly correlated and localized in discrete nuclear foci. LCoR and LCoR2 strongly repressed transcription, inhibited estrogen-induced target gene expression and decreased breast cancer cell proliferation...
August 17, 2017: Oncogene
https://www.readbyqxmd.com/read/28381738/a-new-missense-mutation-in-the-paired-domain-of-the-mouse-pax3-gene
#15
Tamio Ohno, Tomoki Maegawa, Hiroto Katoh, Yuki Miyasaka, Miyako Suzuki, Misato Kobayashi, Fumihiko Horio
Mice with dominant white spotting occurred spontaneously in the C3.NSY-(D11Mit74-D11Mit229) strain. Linkage analysis indicated that the locus for white spotting was located in the vicinity of the Pax3 gene on chromosome 1. Crosses of white-spotted mice showed that homozygosity for the mutation caused tail and limb abnormalities and embryonic lethality as a result of exencephaly; these phenotypes were analogous to those found in other Pax3 mutants. Sequence analysis identified a missense point mutation (c.101G>A) in exon 2 of Pax3 that resulted in a methionine to isoleucine conversion at amino acid 62 of the PAX3 protein...
August 5, 2017: Experimental Animals
https://www.readbyqxmd.com/read/28337770/hypoglycemic-effect-of-hawthorn-in-type-ii-diabetes-mellitus-rat-model
#16
Aili Aierken, Tina Buchholz, Chen Chen, Xiaoying Zhang, Matthias F Melzig
BACKGROUND: Hawthorn is a popular herb in many different traditional medicine systems, including traditional Chinese medicine, where it has long been used for the treatment of hyperglycemia. However, most of its varied biological activities remain unexplored. This study investigated the hypoglycemic effect of hawthorn extracts in type II diabetic (T2DM) rat model. A total of 54 rats were randomly divided into six groups: normal control group; type II diabetic model group (T2DM; these rats were induced by high-fat diet and streptozotocin); high, middle and low concentrations of hawthorn treatment (HTH , HTM and HTL T2DM rats were given hawthorn extract at a dose of 50, 100 and 200 mg kg(-1) body weight, respectively); and positive control group (orlistat 40 mg kg(-1) body weight)...
March 23, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28231311/handwashing-and-ebola-virus-disease-outbreaks-a-randomized-comparison-of-soap-hand-sanitizer-and-0-05-chlorine-solutions-on-the-inactivation-and-removal-of-model-organisms-phi6-and-e-coli-from-hands-and-persistence-in-rinse-water
#17
RANDOMIZED CONTROLLED TRIAL
Marlene K Wolfe, Karin Gallandat, Kyle Daniels, Anne Marie Desmarais, Pamela Scheinman, Daniele Lantagne
To prevent Ebola transmission, frequent handwashing is recommended in Ebola Treatment Units and communities. However, little is known about which handwashing protocol is most efficacious. We evaluated six handwashing protocols (soap and water, alcohol-based hand sanitizer (ABHS), and 0.05% sodium dichloroisocyanurate, high-test hypochlorite, and stabilized and non-stabilized sodium hypochlorite solutions) for 1) efficacy of handwashing on the removal and inactivation of non-pathogenic model organisms and, 2) persistence of organisms in rinse water...
2017: PloS One
https://www.readbyqxmd.com/read/28115546/thin-as-a-versatile-domain-of-transcriptional-repressors-and-catalytic-enzymes-of-thiamine-biosynthesis
#18
Sungmin Hwang, Bryan Cordova, Merna Abdo, Friedhelm Pfeiffer, Julie A Maupin-Furlow
Thiamine biosynthesis is commonly regulated by a riboswitch mechanism; however, the enzymatic steps and regulation of this pathway in archaea are poorly understood. Haloferax volcanii, one of the representative archaea, uses a eukaryote-like Thi4 (thiamine thiazole synthase) for the production of the thiazole ring and condenses this ring with a pyrimidine moiety synthesized by an apparent bacterium-like ThiC (2-methyl-4-amino-5-hydroxymethylpyrimidine [HMP] phosphate synthase) branch. Here we found that archaeal Thi4 and ThiC were encoded by leaderless transcripts, ruling out a riboswitch mechanism...
April 1, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28094941/impact-of-turn-propensity-on-the-folding-rates-of-z34c-protein-implications-for-the-folding-of-helix-turn-helix-motif
#19
Shubhangi Gupta, Yellamraju U Sasidhar
The rate-limiting step for the folding of the helix-turn-helix (HTH) protein, Z34C, involves β-turn region (20)DPNL(23). This reverse turn has been observed to be part of the transition state in the folding process for Z34C, influencing its folding rates. Molecular dynamics simulations were performed on this turn peptide and its two mutants, D20A and P21A, to study turn formation using GROMOS54A7 force field. We find that this region has a turn propensity of its own, and the highest turn propensity is observed for the wild-type, which correlates well with available experimental results...
February 3, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28045389/crystal-structure-of-an-iclr-homologue-from-microbacterium-sp-strain-hm58-2
#20
Tomonori Akiyama, Yusuke Yamada, Naoki Takaya, Shinsaku Ito, Yasuyuki Sasaki, Shunsuke Yajima
The bacterial transcription factor IclR (isocitrate lyase regulator) is a member of a one-component signal transduction system, which shares the common motif of a helix-turn-helix (HTH)-type DNA-binding domain (DBD) connected to a substrate-binding domain (SBD). Here, the crystal structure of an IclR homologue (Mi-IclR) from Microbacterium sp. strain HM58-2, which catabolizes acylhydrazide as the sole carbon source, is reported. Mi-IclR is expected to regulate an operon responsible for acylhydrazide degradation as an initial step...
January 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
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