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https://www.readbyqxmd.com/read/28414308/complex-regulation-of-lcor-signaling-in-breast-cancer-cells
#1
S Jalaguier, C Teyssier, T Nait Achour, A Lucas, S Bonnet, C Rodriguez, N Elarouci, M Lapierre, V Cavaillès
Ligand-dependent corepressor (LCoR) is a transcriptional repressor of ligand-activated estrogen receptors (ERs) and other transcription factors that acts both by recruiting histone deacetylases and C-terminal binding proteins. Here, we first studied LCOR gene expression in breast cancer cell lines and tissues. We detected two mRNAs variants, LCoR and LCoR2 (which encodes a truncated LCoR protein). Their expression was highly correlated and localized in discrete nuclear foci. LCoR and LCoR2 strongly repressed transcription, inhibited estrogen-induced target gene expression and decreased breast cancer cell proliferation...
April 17, 2017: Oncogene
https://www.readbyqxmd.com/read/28381738/a-new-missense-mutation-in-the-paired-domain-of-the-mouse-pax3-gene
#2
Tamio Ohno, Tomoki Maegawa, Hiroto Katoh, Yuki Miyasaka, Miyako Suzuki, Misato Kobayashi, Fumihiko Horio
Mice with dominant white spotting occurred spontaneously in the C3.NSY-(D11Mit74-D11Mit229) strain. Linkage analysis indicated that the locus for white spotting was located in the vicinity of the Pax3 gene on chromosome 1. Crosses of white-spotted mice showed that homozygosity for the mutation caused tail and limb abnormalities and embryonic lethality as a result of exencephaly; these phenotypes were analogous to those found in other Pax3 mutants. Sequence analysis identified a missense point mutation (c.101G>A) in exon 2 of Pax3 that resulted in a methionine to isoleucine conversion at amino acid 62 of the PAX3 protein...
April 6, 2017: Experimental Animals
https://www.readbyqxmd.com/read/28337770/hypoglycemic-effect-of-hawthorn-in-type-ii-diabetes-mellitus-rat-model
#3
Aili Aierken, Tina Buchholz, Chen Chen, Xiaoying Zhang, Matthias F Melzig
BACKGROUND: Hawthorn is one of the popular herbs in many different traditional medicine systems including in Traditional Chinese Medicine (TCM), where it has long been used for the treatment of hyperglycemia. However, most of its varied biological activities are still remaining unexplored. This study investigated the hypoglycemic effect of hawthorn extracts in type II diabetic (T2DM) rat model. A total of 54 rats were randomly divided into six groups. Normal control group (NC), type II diabetic model group (T2DM, T2DM rats were induced by high fat diet and streptozotocin), high, middle and low concentrations of hawthorn treat groups (HTH , HTM and HTL , T2DM rats were given hawthorn extract at the dose of 50, 100 and 200 mg kg ^-1...
March 23, 2017: Journal of the Science of Food and Agriculture
https://www.readbyqxmd.com/read/28231311/handwashing-and-ebola-virus-disease-outbreaks-a-randomized-comparison-of-soap-hand-sanitizer-and-0-05-chlorine-solutions-on-the-inactivation-and-removal-of-model-organisms-phi6-and-e-coli-from-hands-and-persistence-in-rinse-water
#4
Marlene K Wolfe, Karin Gallandat, Kyle Daniels, Anne Marie Desmarais, Pamela Scheinman, Daniele Lantagne
To prevent Ebola transmission, frequent handwashing is recommended in Ebola Treatment Units and communities. However, little is known about which handwashing protocol is most efficacious. We evaluated six handwashing protocols (soap and water, alcohol-based hand sanitizer (ABHS), and 0.05% sodium dichloroisocyanurate, high-test hypochlorite, and stabilized and non-stabilized sodium hypochlorite solutions) for 1) efficacy of handwashing on the removal and inactivation of non-pathogenic model organisms and, 2) persistence of organisms in rinse water...
2017: PloS One
https://www.readbyqxmd.com/read/28115546/thin-as-a-versatile-domain-of-transcriptional-repressors-and-catalytic-enzymes-of-thiamine-biosynthesis
#5
Sungmin Hwang, Bryan Cordova, Merna Abdo, Friedhelm Pfeiffer, Julie A Maupin-Furlow
Thiamine biosynthesis is commonly regulated by a riboswitch mechanism; however, the enzymatic steps and regulation of this pathway in archaea are poorly understood. Haloferax volcanii, one of the representative archaea, uses a eukaryote-like Thi4 (thiamine thiazole synthase) for the production of the thiazole ring and condenses this ring with a pyrimidine moiety synthesized by an apparent bacterium-like ThiC (2-methyl-4-amino-5-hydroxymethylpyrimidine [HMP] phosphate synthase) branch. Here we found that archaeal Thi4 and ThiC were encoded by leaderless transcripts, ruling out a riboswitch mechanism...
April 1, 2017: Journal of Bacteriology
https://www.readbyqxmd.com/read/28094941/impact-of-turn-propensity-on-the-folding-rates-of-z34c-protein-implications-for-the-folding-of-helix-turn-helix-motif
#6
Shubhangi Gupta, Yellamraju U Sasidhar
The rate-limiting step for the folding of the helix-turn-helix (HTH) protein, Z34C, involves β-turn region (20)DPNL(23). This reverse turn has been observed to be part of the transition state in the folding process for Z34C, influencing its folding rates. Molecular dynamics simulations were performed on this turn peptide and its two mutants, D20A and P21A, to study turn formation using GROMOS54A7 force field. We find that this region has a turn propensity of its own, and the highest turn propensity is observed for the wild-type, which correlates well with available experimental results...
February 3, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28045389/crystal-structure-of-an-iclr-homologue-from-microbacterium-sp-strain-hm58-2
#7
Tomonori Akiyama, Yusuke Yamada, Naoki Takaya, Shinsaku Ito, Yasuyuki Sasaki, Shunsuke Yajima
The bacterial transcription factor IclR (isocitrate lyase regulator) is a member of a one-component signal transduction system, which shares the common motif of a helix-turn-helix (HTH)-type DNA-binding domain (DBD) connected to a substrate-binding domain (SBD). Here, the crystal structure of an IclR homologue (Mi-IclR) from Microbacterium sp. strain HM58-2, which catabolizes acylhydrazide as the sole carbon source, is reported. Mi-IclR is expected to regulate an operon responsible for acylhydrazide degradation as an initial step...
January 1, 2017: Acta Crystallographica. Section F, Structural Biology Communications
https://www.readbyqxmd.com/read/28030544/seeking-clearer-recommendations-for-hand-hygiene-in-communities-facing-ebola-a-randomized-trial-investigating-the-impact-of-six-handwashing-methods-on-skin-irritation-and-dermatitis
#8
Marlene K Wolfe, Emma Wells, Brittany Mitro, Anne Marie Desmarais, Pamela Scheinman, Daniele Lantagne
To prevent disease transmission, 0.05% chlorine solution is commonly recommended for handwashing in Ebola Treatment Units. In the 2014 West Africa outbreak this recommendation was widely extended to community settings, although many organizations recommend soap and hand sanitizer over chlorine. To evaluate skin irritation caused by frequent handwashing that may increase transmission risk in Ebola-affected communities, we conducted a randomized trial with 91 subjects who washed their hands 10 times a day for 28 days...
2016: PloS One
https://www.readbyqxmd.com/read/27989519/the-function-of-appendage-patterning-genes-in-mandible-development-of-the-sexually-dimorphic-stag-beetle
#9
Hiroki Gotoh, Robert A Zinna, Yuki Ishikawa, Hitoshi Miyakawa, Asano Ishikawa, Yasuhiro Sugime, Douglas J Emlen, Laura C Lavine, Toru Miura
One of the defining features of the evolutionary success of insects is the morphological diversification of their appendages, especially mouthparts. Although most insects share a common mouthpart ground plan, there is remarkable diversity in the relative size and shapes of these appendages among different insect lineages. One of the most prominent examples of mouthpart modification can be found in the enlargement of mandibles in stag beetles (Coleoptera, Insecta). In order to understand the proximate mechanisms of mouthpart modification, we investigated the function of appendage-patterning genes in mandibular enlargement during extreme growth of the sexually dimorphic mandibles of the stag beetle Cyclommatus metallifer...
December 16, 2016: Developmental Biology
https://www.readbyqxmd.com/read/27930316/structural-basis-for-glutathione-mediated-activation-of-the-virulence-regulatory-protein-prfa-in-listeria
#10
Michael Hall, Christin Grundström, Afshan Begum, Mikael J Lindberg, Uwe H Sauer, Fredrik Almqvist, Jörgen Johansson, A Elisabeth Sauer-Eriksson
Infection by the human bacterial pathogen Listeria monocytogenes is mainly controlled by the positive regulatory factor A (PrfA), a member of the Crp/Fnr family of transcriptional activators. Published data suggest that PrfA requires the binding of a cofactor for full activity, and it was recently proposed that glutathione (GSH) could fulfill this function. Here we report the crystal structures of PrfA in complex with GSH and in complex with GSH and its cognate DNA, the hly operator PrfA box motif. These structures reveal the structural basis for a GSH-mediated allosteric mode of activation of PrfA in the cytosol of the host cell...
December 20, 2016: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/27924027/crystal-structure-of-pseudomonas-aeruginosa-rsal-bound-to-promoter-dna-reaffirms-its-role-as-a-global-regulator-involved-in-quorum-sensing
#11
Huaping Kang, Jianhua Gan, Jingru Zhao, Weina Kong, Jing Zhang, Miao Zhu, Fan Li, Yaqin Song, Jin Qin, Haihua Liang
Pseudomonas aeruginosa possesses at least three well-defined quorum-sensing (QS) (las, rhl and pqs) systems that control a variety of important functions including virulence. RsaL is a QS repressor that reduces QS signal production and ensures homeostasis by functioning in opposition to LasR. However, its regulatory role in signal homeostasis remains elusive. Here, we conducted a ChIP-seq assay and revealed that RsaL bound to two new targets, the intergenic regions of PA2228/PA2229 and pqsH/cdpR, which are required for PQS synthesis...
January 25, 2017: Nucleic Acids Research
https://www.readbyqxmd.com/read/27907189/structural-insights-into-streptococcal-competence-regulation-by-the-cell-to-cell-communication-system-comrs
#12
Antoine Talagas, Laetitia Fontaine, Laura Ledesma-García, Johann Mignolet, Inès Li de la Sierra-Gallay, Noureddine Lazar, Magali Aumont-Nicaise, Michael J Federle, Gerd Prehna, Pascal Hols, Sylvie Nessler
In Gram-positive bacteria, cell-to-cell communication mainly relies on extracellular signaling peptides, which elicit a response either indirectly, by triggering a two-component phosphorelay, or directly, by binding to cytoplasmic effectors. The latter comprise the RNPP family (Rgg and original regulators Rap, NprR, PrgX and PlcR), whose members regulate important bacterial processes such as sporulation, conjugation, and virulence. RNPP proteins are increasingly considered as interesting targets for the development of new antibacterial agents...
2016: PLoS Pathogens
https://www.readbyqxmd.com/read/27754140/os-27-04-prediction-of-left-ventricular-dystolic-dysfunction-in-patient-with-arterial-hypertension
#13
Krasimira Hristova, Vera Bogdanova, Rumen Marinov
OBJECTIVE: Arterial hypertension (HTN) causes left ventricular (LV) dysfunction even if ejection function (EF) remains preserved. The objective of this study was to assess whether complex LAFI (left atrium functional index) had additional value in definition and grading of diastolic dysfunction and compare with indices of atrial deformation - longitudinal strain. DESIGN AND METHOD: Peak atrial longitudinal strain (PALS) was evaluated in 82 patients with systemic HTN and preserved EF (> = 55%) divided in 41 with diastolic dysfunction but no hypertrophy (HTNdd), and 41 with no diastolic dysfunction or hypertrophy (eHTN)...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27741553/structural-basis-for-the-transcriptional-repressor-nicr2-in-nicotine-degradation-from-pseudomonas
#14
Kunzhi Zhang, Geng Wu, Hongzhi Tang, Chuanming Hu, Ting Shi, Ping Xu
Nicotine is an environmental toxicant in tobacco wastes, imposing severe hazards for the health of human and other mammalians. NicR2, a TetR-like repressor from Pseudomonas putida S16, plays a critical role in regulating nicotine degradation. Here, we determined the crystal structures of NicR2 and its complex with the inducer 6-hydroxy-3-succinoyl-pyridine (HSP). The N-terminal domain of NicR2 contains a conserved helix-turn-helix (HTH) DNA-binding motif, while the C-terminal domain contains a cleft for its selective recognition for HSP...
January 2017: Molecular Microbiology
https://www.readbyqxmd.com/read/27692964/the-preserved-hth-docking-cleft-of-hiv-1-integrase-is-functionally-critical
#15
Meytal Galilee, Elena Britan-Rosich, Sarah L Griner, Serdar Uysal, Viola Baumgärtel, Don C Lamb, Anthony A Kossiakoff, Moshe Kotler, Robert M Stroud, Ailie Marx, Akram Alian
HIV-1 integrase (IN) catalyzes viral DNA integration into the host genome and facilitates multifunctional steps including virus particle maturation. Competency of IN to form multimeric assemblies is functionally critical, presenting an approach for anti-HIV strategies. Multimerization of IN depends on interactions between the distinct subunit domains and among the flanking protomers. Here, we elucidate an overlooked docking cleft of IN core domain that anchors the N-terminal helix-turn-helix (HTH) motif in a highly preserved and functionally critical configuration...
November 1, 2016: Structure
https://www.readbyqxmd.com/read/27686023/structural-and-biophysical-properties-of-h-fanci-arm-repeat-protein
#16
Mohd Quadir Siddiqui, Rajan Kumar Choudhary, Pankaj Thapa, Neha Kulkarni, Yogendra S Rajpurohit, Hari S Misra, Nikhil Gadewal, Satish Kumar, Syed K Hasan, Ashok K Varma
Fanconi anemia complementation groups - I (FANCI) protein facilitates DNA ICL (Inter-Cross-link) repair and plays a crucial role in genomic integrity. FANCI is a 1328 amino acids protein which contains armadillo (ARM) repeats and EDGE motif at the C-terminus. ARM repeats are functionally diverse and evolutionarily conserved domain that plays a pivotal role in protein-protein and protein-DNA interactions. Considering the importance of ARM repeats, we have explored comprehensive in silico and in vitro approach to examine folding pattern...
November 10, 2016: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/27643067/os-27-04-prediction-of-left-ventricular-dystolic-dysfunction-in-patient-with-arterial-hypertension
#17
Krasimira Hristova, Vera Bogdanova, Rumen Marinov
OBJECTIVE: Arterial hypertension (HTN) causes left ventricular (LV) dysfunction even if ejection function (EF) remains preserved. The objective of this study was to assess whether complex LAFI (left atrium functional index) had additional value in definition and grading of diastolic dysfunction and compare with indices of atrial deformation - longitudinal strain. DESIGN AND METHOD: Peak atrial longitudinal strain (PALS) was evaluated in 82 patients with systemic HTN and preserved EF (> = 55%) divided in 41 with diastolic dysfunction but no hypertrophy (HTNdd), and 41 with no diastolic dysfunction or hypertrophy (eHTN)...
September 2016: Journal of Hypertension
https://www.readbyqxmd.com/read/27630031/continuous-quality-improvement-efforts-increase-survival-with-favorable-neurologic-outcome-after-out-of-hospital-cardiac-arrest
#18
Karl Sporer, Michael Jacobs, Leo Derevin, Sue Duval, James Pointer
OBJECTIVE: To assess system-wide implementation of specific therapies focused on perfusion during cardiopulmonary resuscitation (CPR) and cerebral recovery after Return of Spontaneous Circulation (ROSC). METHODS: Before and after retrospective analysis of an out-of-hospital cardiac arrest database. Implementation trial in the urban/suburban community of Alameda County, California, USA, population 1.6 million, from November 2009-December 2012. Adult patients with non-traumatic out-of-hospital cardiac arrest (OHCA) who received CPR and/or defibrillation...
September 14, 2016: Prehospital Emergency Care
https://www.readbyqxmd.com/read/27626238/a-model-of-the-spatio-temporal-dynamics-of-drosophila-eye-disc-development
#19
Patrick Fried, Máximo Sánchez-Aragón, Daniel Aguilar-Hidalgo, Birgitta Lehtinen, Fernando Casares, Dagmar Iber
Patterning and growth are linked during early development and have to be tightly controlled to result in a functional tissue or organ. During the development of the Drosophila eye, this linkage is particularly clear: the growth of the eye primordium mainly results from proliferating cells ahead of the morphogenetic furrow (MF), a moving signaling wave that sweeps across the tissue from the posterior to the anterior side, that induces proliferating cells anterior to it to differentiate and become cell cycle quiescent in its wake...
September 2016: PLoS Computational Biology
https://www.readbyqxmd.com/read/27622187/parb-partition-proteins-complex-formation-and-spreading-at-bacterial-and-plasmid-centromeres
#20
REVIEW
Barbara E Funnell
In bacteria, active partition systems contribute to the faithful segregation of both chromosomes and low-copy-number plasmids. Each system depends on a site-specific DNA binding protein to recognize and assemble a partition complex at a centromere-like site, commonly called parS. Many plasmid, and all chromosomal centromere-binding proteins are dimeric helix-turn-helix DNA binding proteins, which are commonly named ParB. Although the overall sequence conservation among ParBs is not high, the proteins share similar domain and functional organization, and they assemble into similar higher-order complexes...
2016: Frontiers in Molecular Biosciences
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