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https://www.readbyqxmd.com/read/28550818/blochsolver-a-gpu-optimized-fast-3d-mri-simulator-for-experimentally-compatible-pulse-sequences
#1
Ryoichi Kose, Katsumi Kose
A magnetic resonance imaging (MRI) simulator, which reproduces MRI experiments using computers, has been developed using two graphic-processor-unit (GPU) boards (GTX 1080). The MRI simulator was developed to run according to pulse sequences used in experiments. Experiments and simulations were performed to demonstrate the usefulness of the MRI simulator for three types of pulse sequences, namely, three-dimensional (3D) gradient-echo, 3D radio-frequency spoiled gradient-echo, and gradient-echo multislice with practical matrix sizes...
May 20, 2017: Journal of Magnetic Resonance
https://www.readbyqxmd.com/read/28550812/molecular-characterization-of-human-adenoviruses-in-urban-wastewaters-using-next-generation-and-sanger-sequencing
#2
M Iaconelli, B Valdazo-González, M Equestre, A R Ciccaglione, C Marcantonio, S Della Libera, G La Rosa
Human adenoviruses (HAdVs) are of major public health importance and are associated with a variety of clinical manifestations, including gastroenteritis, respiratory, ocular and urinary tract infections. To study the occurrence, prevalence and diversity of HAdV species and types circulating in Italy, we conducted a large-scale molecular-epidemiological investigation, a yearlong monitoring of 22 wastewater treatment plants, covering 10 Italian regions, representative of northern, central, and southern Italy...
May 19, 2017: Water Research
https://www.readbyqxmd.com/read/28550784/high-resolution-analyses-reveal-structural-diversity-patterns-of-microbial-communities-in-chromite-ore-processing-residue-copr-contaminated-soils
#3
Xiaobo Min, Yangyang Wang, Liyuan Chai, Zhihui Yang, Qi Liao
To explore how heavy metal contamination in Chromite Ore Processing Residue (COPR) disposal sites determine the dissimilarities of indigenous microbial communities, 16S rRNA gene MiSeq sequencing and advanced statistical methods were applied. 13 soil samples were collected from three COPR disposal sites in Mouding of southwestern, Shangnan of northwestern and Yima of central China. The results of analyses of variance (ANOVA), similarities (ANOSIM), and non-metric multidimensional scaling (NMDS) showed that the structural diversity of the microbial communities in the samples with high total chromium (Cr) content (more than 300 mg kg(-1); High group) were significantly lesser than in the Low group (less than 90 mg kg(-1)) regardless of their geographical distribution...
May 21, 2017: Chemosphere
https://www.readbyqxmd.com/read/28550721/clinical-performance-of-the-veris-hcv-assay-for-hepatitis-c-virus-rna-quantification
#4
Laure Izquierdo, Corinne Prégermain, Corinne Hottelet, Gwenaëlle Decombe, Anne-Marie Roque-Afonso
BACKGROUND: Diagnosis of hepatitis C virus (HCV) infection and treatment monitoring rely on detection/quantification of HCV RNA and real-time polymerase chain reaction (PCR) techniques are expected to equivalently quantify the different HCV genotypes. OBJECTIVE: The clinical performance of the VERIS HCV assay for HCV RNA quantification was compared to that of the Abbott RealTime HCV assay. STUDY DESIGN: Qualitative concordance and quantitative comparison were evaluated on a first panel of 286 clinical samples containing HCV genotypes 1-6...
May 18, 2017: Journal of Clinical Virology: the Official Publication of the Pan American Society for Clinical Virology
https://www.readbyqxmd.com/read/28550687/tanshinone-suppresses-arecoline-induced-epithelial-mesenchymal-transition-in-oral-submucous-fibrosis-by-epigenetically-reactivating-the-p53-pathway
#5
Lian Zheng, Zhen-Jie Guan, Wen-Ting Pan, Tian-Fang Du, Yu-Jia Zhai, Jia Guo
Oral submucous fibrosis (OSF) induced by the chewing of areca nut has been considered as a precancerous lesion with a higher probability of developing oral squamous cell carcinoma. Tanshinone (TSN) is the main component extracted from Salvia miltiorrhiza, a traditional Chinese medicine, which was found to have diverse pharmacological effects such as anti-inflammatory and anti-tumor effects. In current study, we aim to identify inhibitory effects and the underlying mechanism of Tanshinone on OSF progress. We found that treatment of TSN inhibited arecoline-mediated proliferation of primary human oral mucosal fibroblasts, and reversed the promotive effects of arecoline on EMT process...
May 21, 2017: Oncology Research
https://www.readbyqxmd.com/read/28550675/-prognostic-value-of-sequencing-of-radiotherapy-and-chemotherapy-following-breast-conserving-surgery-for-patients-with-breast-cancer
#6
Q Z Zhong, Z Wang, Y Tang, Q L Rong, S L Wang, J Jin, W H Wang, Y P Liu, Y W Song, H Fang, B Chen, S N Qi, N Li, Y Tang, J H Zhang, Y X Li
Objective: To evaluate the prognostic value of sequencing of adjuvant radiotherapy and chemotherapy following breast-conserving surgery for patients with breast cancer. Methods: A total of 1 154 patients withT1-2N0-3M0 breast cancer retrospectively reviewed. All patients received sequential radiotherapy and chemotherapy following breast-conserving surgery. Among them, 603 patients received radiotherapy first and 551 patients received chemotherapy first. Log-rank tests were used to determine significance of disease-free survival (DFS) and overall survival (OS) rates in the Kaplan-Meier curve...
April 23, 2017: Zhonghua Zhong Liu za Zhi [Chinese Journal of Oncology]
https://www.readbyqxmd.com/read/28550649/improved-formula-see-text-determination-in-23-na-35-cl-and-17-o-mri-using-iterative-partial-volume-correction-based-on-1-h-mri-segmentation
#7
Sebastian C Niesporek, Reiner Umathum, Thomas M Fiedler, Peter Bachert, Mark E Ladd, Armin M Nagel
OBJECTIVE: Functional parameters can be measured with the help of quantitative non-proton MRI where exact relaxometry parameters are needed. Investigation of [Formula: see text] is often biased by strong partial volume (PV) effects. Hence, in this work a PV correction algorithm approach was evaluated that uses iteratively adapted [Formula: see text]-values and high-resolution structural (1)H data to determine transverse relaxation in non-proton MRI more accurately. MATERIALS AND METHODS: Simulations, a phantom study and in vivo (23)Na, (17)O and (35)Cl MRI measurements of five healthy volunteers were performed to evaluate the algorithm...
May 26, 2017: Magma
https://www.readbyqxmd.com/read/28550645/new-entamoeba-group-in-howler-monkeys-alouatta-spp-associated-with-parasites-of-reptiles
#8
Claudia Villanueva-García, Elías José Gordillo-Chávez, Carlos Baños-Ojeda, Emilio Rendón-Franco, Claudia Irais Muñoz-García, Julio César Carrero, Alex Córdoba-Aguilar, Pablo Maravilla, José Galian, Fernando Martínez-Hernández, Guiehdani Villalobos
Our knowledge of the parasite species present in wildlife hosts is incomplete. Protozoans such as amoebae of the genus Entamoeba infect a large variety of vertebrate species, including NHPs. However, traditionally, their identification has been accomplished through microscopic evaluation; therefore, amoeba species have not always been identified correctly. We searched for Entamoeba spp. using a fragment of the small subunit rDNA in free-ranging howler monkeys (Alouatta palliata and A. pigra) from southeast Mexico...
May 27, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28550644/high-genetic-diversity-of-giardia-duodenalis-assemblage-e-in-pre-weaned-dairy-calves-in-shanghai-china-revealed-by-multilocus-genotyping
#9
Xiaolan Wang, Min Cai, Wen Jiang, Yuping Wang, Yue Jin, Na Li, Yaqiong Guo, Yaoyu Feng, Lihua Xiao
Giardia duodenalis is a common parasitic protozoan in human and animals. Epidemiological and molecular data are available from dairy cattle in many industrialized countries, but information on genetic diversity at multiple genetic loci is limited, especially in pre-weaned dairy calves. In this study, 818 fecal specimens were collected from five dairy cattle farms located in suburbs of Shanghai, China, with two to five samplings per farm. G. duodenalis assemblages and subtypes were determined using multilocus genotyping (MLG) at the β-giardin (bg), glutamate dehydrogenase (gdh), and triosephosphate isomerase (tpi) loci...
May 26, 2017: Parasitology Research
https://www.readbyqxmd.com/read/28550592/certolizumab-pegol-for-treating-rheumatoid-arthritis-following-inadequate-response-to-a-tnf-%C3%AE-inhibitor-an-evidence-review-group-perspective-of-a-nice-single-technology-appraisal
#10
REVIEW
Iñigo Bermejo, Matt Stevenson, Rachel Archer, John W Stevens, Edward Goka, Mark Clowes, David L Scott, Adam Young
As part of its single technology appraisal (STA) process, the National Institute for Health and Care Excellence (NICE) invited the manufacturer (UCB Pharma) of certolizumab pegol (CZP; Cimzia(®)) to submit evidence of its clinical and cost effectiveness for the treatment of rheumatoid arthritis (RA) following inadequate response to a tumour necrosis factor-α inhibitor (TNFi). The School of Health and Related Research Technology Appraisal Group at the University of Sheffield was commissioned to act as the independent Evidence Review Group (ERG)...
May 26, 2017: PharmacoEconomics
https://www.readbyqxmd.com/read/28550590/exome-sequencing-identifies-candidate-genetic-modifiers-of-syndromic-and-familial-thoracic-aortic-aneurysm-severity
#11
Benjamin J Landis, Jeffrey A Schubert, Dongbing Lai, Anil G Jegga, Amy R Shikany, Tatiana Foroud, Stephanie M Ware, Robert B Hinton
Thoracic aortic aneurysm (TAA) is a genetic disease predisposing to aortic dissection. It is important to identify the genetic modifiers controlling penetrance and expressivity to improve clinical prognostication. Exome sequencing was performed in 27 subjects with syndromic or familial TAA presenting with extreme phenotypes (15 with severe TAA; 12 with mild or absent TAA). Family-based analysis of a subset of the cohort identified variants, genes, and pathways segregating with TAA severity among three families...
May 26, 2017: Journal of Cardiovascular Translational Research
https://www.readbyqxmd.com/read/28550586/stress-echocardiography-with-smartphone-real-time-remote-reading-for-regional-wall-motion
#12
Maria Chiara Scali, Clarissa Carmona de Azevedo Bellagamba, Quirino Ciampi, Iana Simova, José Luis de Castro E Silva Pretto, Ana Djordjevic-Dikic, Claudio Dodi, Lauro Cortigiani, Angela Zagatina, Paolo Trambaiolo, Marco R Torres, Rodolfo Citro, Paolo Colonna, Marco Paterni, Eugenio Picano
The diffusion of smart-phones offers access to the best remote expertise in stress echo (SE). To evaluate the reliability of SE based on smart-phone filming and reading. A set of 20 SE video-clips were read in random sequence with a multiple choice six-answer test by ten readers from five different countries (Italy, Brazil, Serbia, Bulgaria, Russia) of the "SE2020" study network. The gold standard to assess accuracy was a core-lab expert reader in agreement with angiographic verification (0 = wrong, 1 = right)...
May 26, 2017: International Journal of Cardiovascular Imaging
https://www.readbyqxmd.com/read/28550527/environmental-yeast-communities-in-vineyards-in-the-mountains-of-santa-catarina-state-brazil
#13
S D C Mendes, M Ramírez-Castrillón, N P Feldberg, F C Bertoldi, P Valente
Yeasts were isolated from three vineyards located in the South Region of Brazil. A cross evaluation was carried out at the oldest vineyard of the study in Pinheiro Preto. Samples of grape berries, grapevine leaves and the soil, along with samples of the winery equipment and effluent, were collected. In the Serra do Marari and Campos Novos vineyards only samples of grape clusters were obtained. The 106 yeast isolates were identified by sequencing the D1/D2 domain of LSU rDNA or ITS1-5.8S-ITS2 region in 22 species...
June 2017: World Journal of Microbiology & Biotechnology
https://www.readbyqxmd.com/read/28550501/grass-carp-reovirus-gd108-fiber-protein-is-involved-in-cell-attachment
#14
Yuanyuan Tian, Zhenzhen Jiao, Junjian Dong, Chengfei Sun, Xiaoyan Jiang, Xing Ye
Viral attachment to specific host receptors is the first step in viral infection and serves an essential function in the selection of target cells. In this study, structure analysis, neutralization assays, and cell attachment assays were carried out to evaluate the cell attachment functions of the outer capsid fiber protein of grass carp reovirus GD108 strain (GCRV-GD108). The GCRV-GD108 fiber protein contained 512 amino acids encoded by S7 segment and shared sequence similarities with mammalian reovirus cell attachment protein σ1 and adenovirus fiber...
May 26, 2017: Virus Genes
https://www.readbyqxmd.com/read/28550499/systematic-review-of-fmri-compatible-devices-design-and-testing-criteria
#15
REVIEW
V Hartwig, N Carbonaro, A Tognetti, N Vanello
Functional Magnetic Resonance Imaging (fMRI) is at present one of the most used methodologies for functional brain exploration, both in clinical and research settings. fMRI can noninvasively measure neural activity by using specific experimental paradigms. Often, these paradigms require the stimulation of the subject to perform sensorimotor tasks: in the past, the stimuli have been administered manually for investigating fundamental aspects of tactile perception and somatosensory processing. Nowadays, the use of mechatronic devices to stimulate the subject during fMRI studies is growing, also to assure reproducibility, control, and monitoring of task performances...
May 26, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28550470/hormonal-and-morphological-changes-during-seed-development-of-cypripedium-japonicum
#16
Xiaona Yan, Min Tian, Fen Liu, Caixia Wang, Ying Zhang
With the goal of evaluating the factors that may be influencing the reproductive success of an endangered, terrestrial orchid Cypripedium japonicum Thunb., the anatomical and morphological characteristics of seed development were assessed, as were the levels of endogenous hormones to investigate the possible relationship between hormones and seed development. The results showed that morphological characteristics of seeds were changing from milky white and filiform to brown and fusiform. The development of ovule that initiated on the fertile valves only began after pollination, and a mature embryo sac was produced at 40 days after pollination (DAP)...
May 27, 2017: Protoplasma
https://www.readbyqxmd.com/read/28550453/exchange-of-genetic-sequences-between-viruses-and-hosts
#17
Robin A Weiss
Although genetic transfer between viruses and vertebrate hosts occurs less frequently than gene flow between bacteriophages and prokaryotes, it is extensive and has affected the evolution of both parties. With retroviruses, the integration of proviral DNA into chromosomal DNA can result in the activation of adjacent host gene expression and in the transduction of host transcripts into retroviral genomes as oncogenes. Yet in contrast to lysogenic phage, there is little evidence that viral oncogenes persist in a chain of natural transmission or that retroviral transduction is a significant driver of the horizontal spread of host genes...
May 28, 2017: Current Topics in Microbiology and Immunology
https://www.readbyqxmd.com/read/28550433/the-biological-features-and-genetic-diversity-of-novel-fish-rhabdovirus-isolates-in-china
#18
Xiaozhe Fu, Qiang Lin, Hongru Liang, Lihui Liu, Zhibin Huang, Ningqiu Li, Jianguo Su
The Rhabdoviridae is a diverse family of negative-sense single-stranded RNA viruses which infects mammals, birds, reptiles, fish, insects and plants. Herein, we reported the isolation and characterization of 6 novel viruses from diseased fish collected from China including SCRV-QY, SCRV-SS, SCRV-GM, CmRV-FS, MsRV-SS, OmbRV-JM. The typical clinical symptom of diseased fish was hemorrhaging. Efficient propagation of these isolates in a Chinese perch brain cell line was determined by means of observation of cytopathic effect, RT-PCR and electron microscopy...
May 26, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28550432/molecular-characterization-of-a-novel-luteovirus-from-peach-identified-by-high-throughput-sequencing
#19
L-P Wu, H-W Liu, M Bateman, Z Liu, R Li
Contigs with sequence homologies to cherry-associated luteovirus were identified by high-throughput sequencing analysis in two peach accessions. Complete genomic sequences of the two isolates of this virus were determined to be 5,819 and 5,814 nucleotides long, respectively. The genome of the new virus is typical of luteoviruses, containing eight open reading frames in a very similar arrangement. Its genomic sequence is 58-74% identical to those of other members of the genus Luteovirus. These sequences thus belong to a new virus, which we have named "peach-associated luteovirus"...
May 26, 2017: Archives of Virology
https://www.readbyqxmd.com/read/28550408/bacillus-pumilus-strain-yspmk11-as-plant-growth-promoter-and-bicontrol-agent-against-sclerotinia-sclerotiorum
#20
Manoj Kaushal, Ajay Kumar, Rajesh Kaushal
A study was executed in a direction to attenuate Sclerotinia stalk rot (SSR) disease through biocontrol agent and also to enhance crop productivity. Culture filtrate of bacterial strain YSPMK11 inhibited growth of Sclerotinia sclerotiorum in vitro which also exhibited higher plant growth promoting attributes. Interaction studies revealed maximum (81.50%) growth inhibition at 35 °C and pH 7.0 after 72 h incubation period with 15% culture filtrate. Based upon 16S rRNA gene sequence strain, YSPMK11 was identified as Bacillus pumilus...
June 2017: 3 Biotech
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