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https://www.readbyqxmd.com/read/28933990/influence-of-salinomycin-treatment-on-division-and-movement-of-individual-cancer-cells-cultured-in-normoxia-or-hypoxia-evaluated-with-time-lapse-digital-holographic-microscopy
#1
Sofia Kamlund, Daniel Strand, Birgit Janicke, Kersti Alm, Stina Oredsson
Most studies on new cancer drugs are based on population-derived data, where the absence of response of a small population may pass unnoticed. Thus, individual longitudinal tracking of cells is important for the future development of efficient cancer treatments. We have used digital holographic microscopy to track individual JIMT-1 human breast cancer cells and L929 mouse fibroblast cultivated in normoxia or hypoxia. In addition, JIMT-1 cells were treated with salinomycin, a cancer stem cell targeting compound...
September 21, 2017: Cell Cycle
https://www.readbyqxmd.com/read/28931937/high-accuracy-label-free-classification-of-single-cell-kinetic-states-from-holographic-cytometry-of-human-melanoma-cells
#2
Miroslav Hejna, Aparna Jorapur, Jun S Song, Robert L Judson
Digital holographic cytometry (DHC) permits label-free visualization of adherent cells. Dozens of cellular features can be derived from segmentation of hologram-derived images. However, the accuracy of single cell classification by these features remains limited for most applications, and lack of standardization metrics has hindered independent experimental comparison and validation. Here we identify twenty-six DHC-derived features that provide biologically independent information across a variety of mammalian cell state transitions...
September 20, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28916149/wavelet-based-tracking-of-bacteria-in-unreconstructed-off-axis-holograms
#3
Zach Marin, J Kent Wallace, Jay Nadeau, Andre Khalil
We propose an automated wavelet-based method of tracking particles in unreconstructed off-axis holograms to provide rough estimates of the presence of motion and particle trajectories in digital holographic microscopy (DHM) time series. The wavelet transform modulus maxima segmentation method is adapted and tailored to extract Airy-like diffraction disks, which represent bacteria, from DHM time series. In this exploratory analysis, the method shows potential for estimating bacterial tracks in low-particle-density time series, based on a preliminary analysis of both living and dead Serratia marcescens, and for rapidly providing a single-bit answer to whether a sample chamber contains living or dead microbes or is empty...
September 12, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28901765/a-simple-and-versatile-strategy-for-rapid-color-fading-and-intense-coloration-of-photochromic-naphthopyran-families
#4
Yuki Inagaki, Yoichi Kobayashi, Katsuya Mutoh, Jiro Abe
Benzo-annulated chromenes, i.e., naphthopyrans, are well-known photochromic molecules that undergo photochemical ring-opening reactions to form two colored open-ring isomers, the transoid-cis and transoid-trans forms, upon light irradiation. Though the transoid-cis form returns thermally to the uncolored closed form, the fading rate of the transoid-trans form is extremely slow because of its higher thermal stability. This slow fading behavior of the transoid-trans form is responsible for the persistence of residual color for several minutes to hours, and prevents the application of such molecules to fast photoswitching materials...
September 13, 2017: Journal of the American Chemical Society
https://www.readbyqxmd.com/read/28898048/dielectric-meta-holograms-enabled-with-dual-magnetic-resonances-in-visible-light
#5
Zile Li, Inki Kim, Lei Zhang, Muhammad Q Mehmood, Muhammad S Anwar, Murtaza Saleem, Dasol Lee, Ki Tae Nam, Shuang Zhang, Boris Luk'yanchuk, Yu Wang, Guoxing Zheng, Junsuk Rho, Cheng-Wei Qiu
Efficient transmission-type meta-holograms have been demonstrated using high-index dielectric nanostructures based on Huygens' principle. It is crucial that the geometry size of building blocks be judiciously optimized individually for spectral overlap of electric and magnetic dipoles. In contrast, reflection-type meta-holograms using the metal/insulator/metal scheme and geometric phase can be readily achieved with high efficiency and small thickness. Here, we demonstrate a general platform for design of dual magnetic resonance based meta-holograms based on the geometric phase using silicon nanostructures that are quarter wavelength thick for visible light...
September 14, 2017: ACS Nano
https://www.readbyqxmd.com/read/28894326/calcite-dissolution-rate-spectra-measured-by-in-situ-digital-holographic-microscopy
#6
Alexander S Brand, Pan Feng, Jeffrey W Bullard
Digital holographic microscopy in reflection mode is used to track in situ, real-time nanoscale topography evolution of cleaved (104) calcite surfaces exposed to flowing or static deionized water. The method captures full-field holograms of the surface at frame rates of up to 12.5 s(-1). Numerical reconstruction provides 3D surface topography with vertical resolution of a few nanometers and enables measurement of time-dependent local dissolution fluxes. A statistical distribution, or spectrum, of dissolution rates is generated by sampling multiple area domains on multiple crystals...
September 2017: Geochimica et Cosmochimica Acta
https://www.readbyqxmd.com/read/28878232/holographic-writing-of-ink-based-phase-conjugate-nanostructures-via-laser-ablation
#7
Muhammad Waqas Khalid, Rajib Ahmed, Ali K Yetisen, Bader AlQattan, Haider Butt
The optical phase conjugation (OPC) through photonic nanostructures in coherent optics involves the utilization of a nonlinear optical mechanism through real-time processing of electromagnetic fields. Their applications include spectroscopy, optical tomography, wavefront sensing, and imaging. The development of functional and personalized holographic devices in the visible and near-infrared spectrum can be improved by introducing cost-effective, rapid, and high-throughput fabrication techniques and low-cost recording media...
September 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28874744/digital-holographic-high-speed-3d-imaging-for-the-vibrometry-of-fast-occurring-phenomena
#8
Takashi Kakue, Yutaka Endo, Takashi Nishitsuji, Tomoyoshi Shimobaba, Nobuyuki Masuda, Tomoyoshi Ito
Digital holography allows production of high-speed three-dimensional images at rates over 100,000 frames per second; however, simultaneously obtaining suitable performance and levels of accuracy using digital holography is difficult. This problem prevents high-speed three-dimensional imaging from being used for vibrometry. In this paper, we propose and test a digital holography method that can produce vibration measurements. The method is based on single-shot phase-shifting interferometry. Herein, we imaged the surface of a loudspeaker diaphragm and measured its displacement due to the vibrations produced by a frequency sweep signal...
September 5, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28871109/two-color-pump-probe-interferometry-of-ultra-fast-light-matter-interaction
#9
Yoshio Hayasaki, Shin-Ichi Fukuda, Satoshi Hasegawa, Saulius Juodkazis
Two-color side-view probing of light-matter interaction from minute focal volume of a tightly focused fs-laser pump pulse reveals charge dynamics with high 0.9 μm optical resolution and approximately ~45fs temporal resolution defined by pulse duration. Use of two colors is advantageous for probing optically excited plasma regions with different density. Holographical digital focusing and spatial filtering were implemented to obtain the same resolution images for subsequent Fourier analysis. Fast plasma density decay with time constant ~150 fs was resolved and is consistent with self-trapping...
September 4, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28864356/lensless-digital-holographic-microscopy-and-its-applications-in-biomedicine-and-environmental-monitoring
#10
REVIEW
Yichen Wu, Aydogan Ozcan
Optical compound microscope has been a major tool in biomedical imaging for centuries. Its performance relies on relatively complicated, bulky and expensive lenses and alignment mechanics. In contrast, the lensless microscope digitally reconstructs microscopic images of specimens without using any lenses, as a result of which it can be made much smaller, lighter and lower-cost. Furthermore, the limited space-bandwidth product of objective lenses in a conventional microscope can be significantly surpassed by a lensless microscope...
August 29, 2017: Methods: a Companion to Methods in Enzymology
https://www.readbyqxmd.com/read/28846747/distinctive-behaviour-of-live-biopsy-derived-carcinoma-cells-unveiled-using-coherence-controlled-holographic-microscopy
#11
Břetislav Gál, Miroslav Veselý, Jana Čolláková, Marta Nekulová, Veronika Jůzová, Radim Chmelík, Pavel Veselý
Head and neck squamous cell carcinoma is one of the most aggressive tumours and is typically diagnosed too late. Late diagnosis requires an urgent decision on an effective therapy. An individualized test of chemosensitivity should quickly indicate the suitability of chemotherapy and radiotherapy. No ex vivo chemosensitivity assessment developed thus far has become a part of general clinical practice. Therefore, we attempted to explore the new technique of coherence-controlled holographic microscopy to investigate the motility and growth of live cells from a head and neck squamous cell carcinoma biopsy...
2017: PloS One
https://www.readbyqxmd.com/read/28841322/dissolution-kinetics-of-cubic-tricalcium-aluminate-measured-by-digital-holographic-microscopy
#12
Alexander S Brand, Jeffrey W Bullard
In situ digital holographic microscopy is used to characterize the dissolution flux of polycrystalline cubic tricalcium aluminate (C3A-c). The surface dissolves at rates that vary considerably with time and spatial location. This implies a statistical distribution of fluxes, but an approximately steady-state median rate was obtained by using flowing solutions and by reducing the water activity in the solution. The dissolution flux from highly crystalline C3A-c depends on the water activity raised to an empirically derived exponent of 5...
September 7, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28836416/automated-classification-of-cell-morphology-by-coherence-controlled-holographic-microscopy
#13
Lenka Strbkova, Daniel Zicha, Pavel Vesely, Radim Chmelik
In the last few years, classification of cells by machine learning has become frequently used in biology. However, most of the approaches are based on morphometric (MO) features, which are not quantitative in terms of cell mass. This may result in poor classification accuracy. Here, we study the potential contribution of coherence-controlled holographic microscopy enabling quantitative phase imaging for the classification of cell morphologies. We compare our approach with the commonly used method based on MO features...
August 2017: Journal of Biomedical Optics
https://www.readbyqxmd.com/read/28827855/in-situ-nanoscale-observations-of-gypsum-dissolution-by-digital-holographic-microscopy
#14
Pan Feng, Alexander S Brand, Lei Chen, Jeffrey W Bullard
Recent topography measurements of gypsum dissolution have not reported the absolute dissolution rates, but instead focus on the rates of formation and growth of etch pits. In this study, the in situ absolute retreat rates of gypsum (010) cleavage surfaces at etch pits, at cleavage steps, and at apparently defect-free portions of the surface are measured in flowing water by reflection digital holographic microscopy. Observations made on randomly sampled fields of view on seven different cleavage surfaces reveal a range of local dissolution rates, the local rate being determined by the topographical features at which material is removed...
June 2017: Chemical Geology
https://www.readbyqxmd.com/read/28822008/label-free-sensing-and-classification-of-old-stored-blood
#15
Jun Hong Park, Taesik Go, Sang Joon Lee
Transfusion is crucial in surgical operation and anemia treatment. However, several hemorheological properties of blood are adversely altered during blood storage. After transfusion, these adverse alterations are related with decrease of oxygen and ion transport in blood circulation, which increase the mortality of transfused patients. Therefore, accurate sensing of whether a blood supply is still viable for transfusion or not is extremely important. In this study, a H-shaped microfluidic device and digital in-line holographic microscopy were employed to measure temporal variations of blood viscosity and the optical focusing property of erythrocytes during blood storage...
August 18, 2017: Annals of Biomedical Engineering
https://www.readbyqxmd.com/read/28819645/3d-imaging-of-optically-cleared-tissue-using-a-simplified-clarity-method-and-on-chip-microscopy
#16
Yibo Zhang, Yoonjung Shin, Kevin Sung, Sam Yang, Harrison Chen, Hongda Wang, Da Teng, Yair Rivenson, Rajan P Kulkarni, Aydogan Ozcan
High-throughput sectioning and optical imaging of tissue samples using traditional immunohistochemical techniques can be costly and inaccessible in resource-limited areas. We demonstrate three-dimensional (3D) imaging and phenotyping in optically transparent tissue using lens-free holographic on-chip microscopy as a low-cost, simple, and high-throughput alternative to conventional approaches. The tissue sample is passively cleared using a simplified CLARITY method and stained using 3,3'-diaminobenzidine to target cells of interest, enabling bright-field optical imaging and 3D sectioning of thick samples...
August 2017: Science Advances
https://www.readbyqxmd.com/read/28809910/enhanced-depth-of-field-of-an-integral-imaging-microscope-using-a-bifocal-holographic-optical-element-micro-lens-array
#17
Ki-Chul Kwon, Young-Tae Lim, Chang-Won Shin, Munkh-Uchral Erdenebat, Jae-Moon Hwang, Nam Kim
We propose and implement an integral imaging microscope with extended depth-of-field (DoF) using a bifocal holographic micro lens array (MLA). The properties of the two MLAs are switched via peristrophic multiplexing, where different properties of the MLA are recorded onto the single holographic optical element (HOE). The recorded MLA properties are perpendicular to each other: after the first mode is recorded, the HOE is rotated by 90° clockwise, and the second mode is recorded. The experimental results confirm that the DoF of the integral imaging microscopy system is extended successfully by using the bifocal MLA...
August 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28808271/dynamic-modulation-of-poincar%C3%A3-beams
#18
C Alpmann, C Schlickriede, E Otte, C Denz
Generation of complex Poincaré beams is enabled by amplitude and phase modulation accompanied by simultaneous spatially polarization structuring. A holographic system to tailor complex light fields and optical angular momentum structures forecasts promising applications in quantum communication and optical trapping. Experimental results are presented together with simulations of complex Poincaré beams embedding different types of polarization singularities. Additionally, parameters of the dynamic polarization modulation system are discussed and analyzed to demonstrate the enormous capability of the method...
August 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28806865/glucose-sensitive-holographic-bio-sensors-fundamentals-and-applications
#19
Marjan Heidari, Bahareh Dabirmanesh, Hossein Mahdavi, Khosro Khajeh
Nowadays sensors and especially biosensors play an important role in medical diagnosis and detection of food and environment contaminations. Biosensors’ facilities have been improved significantly by using technologies such as surface plasmon resonance, microfluidics, laser, and electrochemistry. These technologies are now available on chips in micro- and nano-scale and are capable of measuring the quantity of more than one analyte at once. One branch of these sensors is the holographic (bio)sensor. Reflection holographic sensors, which are optical devices fabricated based on Bragg’s law, can diffract light with specific wavelength as they absorb or release special ions or molecule depending on their polymer structure...
August 15, 2017: Iranian Biomedical Journal
https://www.readbyqxmd.com/read/28798957/holographic-deep-learning-for-rapid-optical-screening-of-anthrax-spores
#20
YoungJu Jo, Sangjin Park, JaeHwang Jung, Jonghee Yoon, Hosung Joo, Min-Hyeok Kim, Suk-Jo Kang, Myung Chul Choi, Sang Yup Lee, YongKeun Park
Establishing early warning systems for anthrax attacks is crucial in biodefense. Despite numerous studies for decades, the limited sensitivity of conventional biochemical methods essentially requires preprocessing steps and thus has limitations to be used in realistic settings of biological warfare. We present an optical method for rapid and label-free screening of Bacillus anthracis spores through the synergistic application of holographic microscopy and deep learning. A deep convolutional neural network is designed to classify holographic images of unlabeled living cells...
August 2017: Science Advances
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