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https://www.readbyqxmd.com/read/28520896/laser-assisted-indocyanine-green-dye-angiography-for-postoperative-fistulas-after-salvage-laryngectomy
#1
Erin J Partington, Lindsay S Moore, Russel Kahmke, Jason M Warram, William Carroll, Eben L Rosenthal, Benjamin J Greene
Importance: Pharyngocutaneous fistula formation is an unfortunate complication after salvage laryngectomy for head and neck cancer that is difficult to anticipate and related to a variety of factors, including the viability of native pharyngeal mucosa. Objective: To examine whether noninvasive angiography with indocyanine green (ICG) dye can be used to evaluate native pharyngeal vascularity to anticipate pharyngocutaneous fistula development. Design, Setting, and Participants: This cohort study included 37 patients enrolled from June 1, 2013, to June 1, 2016, and follow-up was for at least 1 month postoperatively...
May 18, 2017: JAMA Otolaryngology—Head & Neck Surgery
https://www.readbyqxmd.com/read/28518011/you-re-the-flight-surgeon
#2
(no author information available yet)
Ensley D. You're the flight surgeon: green laser event. Aerosp Med Hum Perform. 2017; 88(4):437-439.
April 1, 2017: Aerospace Medicine and Human Performance
https://www.readbyqxmd.com/read/28509533/a-low-cost-label-free-biosensing-bimetallic-cellulose-strip-with-silar-synthesized-silver-core-gold-shell-nanoparticle-structures
#3
Wansun Kim, Jae-Chul Lee, Gi-Ja Lee, Hun-Kuk Park, Anbok Lee, Samjin Choi
We introduce a label-free biosensing cellulose strip sensor with surface-enhanced Raman spectroscopy (SERS)-encoded bimetallic core@shell nanoparticles. Bimetallic nanoparticles consisting of a synthesis of core Ag nanoparticles (AgNP) and a synthesis of shell gold nanoparticles (AuNPs) were fabricated on a cellulose substrate by two-stage successive ionic layer absorption and reaction (SILAR) techniques. The bimetallic nanoparticle-enhanced localized surface plasmon resonance (LSPR) effects were theoretically verified by computational calculations with finite element models of optimized bimetallic nanoparticles interacting with an incident laser source...
May 16, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28506879/a-new-platform-for-long-term-tracking-and-recording-of-neural-activity-and-simultaneous-optogenetic-control-in-freely-behaving-caenorhabditis-elegans
#4
Keiko Gengyo-Ando, Yuko Kagawa-Nagamura, Masamichi Ohkura, Xianfeng Fei, Min Chen, Koichi Hashimoto, Junichi Nakai
BACKGROUND: Real-time recording and manipulation of neural activity in freely behaving animals can greatly advance our understanding of how neural circuits regulate behavior. Ca(2+) imaging and optogenetic manipulation with optical probes are key technologies for this purpose. However, integrating the two optical approaches with behavioral analysis has been technically challenging. NEW METHOD: Here, we developed a new imaging system, ICaST (Integrated platform for Ca(2+) imaging, Stimulation, and Tracking), which combines an automatic worm tracking system and a fast-scanning laser confocal microscope, to image neurons of interest in freely behaving C...
May 12, 2017: Journal of Neuroscience Methods
https://www.readbyqxmd.com/read/28501673/chemotherapeutic-drug-photothermal-agent-co-self-assembling-nanoparticles-for-near-infrared-fluorescence-and-photoacoustic-dual-modal-imaging-guided-chemo-photothermal-synergistic-therapy
#5
Yang Li, Guihua Liu, Jinyuan Ma, Jinyan Lin, Huirong Lin, Guanghao Su, Dengyue Chen, Shefang Ye, Xiaoyuan Chen, Xuan Zhu, Zhenqing Hou
Multimodal imaging-guided synergistic combination therapy has shown great potential for cancer treatment. However, the nanocarrier-based theranostic systems suffer from batch-to-batch variation, complexity of multicomponent, poor drug loading, and carrier-related toxicity issues. To address these issues, herein we developed a novel carrier-free theranostic system with nanoscale characteristics for near-infrared fluorescence (NIRF) and photoacoustic (PA) dual-modal imaging-guided synergistic chemo-photothermal therapy (PTT)...
May 10, 2017: Journal of Controlled Release: Official Journal of the Controlled Release Society
https://www.readbyqxmd.com/read/28499097/substantivity-of-carbodiimide-inhibition-on-dentinal-enzyme-activity-over-time
#6
A Mazzoni, V Angeloni, N Sartori, S Duarte, T Maravic, L Tjäderhane, D H Pashley, F R Tay, L Breschi
The use of 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide HCl (EDC) has recently been investigated for its effectiveness in the prevention of collagen degradation over time and the improvement of resin-dentin bond durability. The objective of the present study was to evaluate the effects of a 0.3 M EDC-containing conditioner on endogenous enzymatic activities within the hybrid layer (HL) created by a self-etch or an etch-and-rinse adhesive after 1 y. The activity within the HL was examined using in situ zymography and confocal laser scanning microscopy after 24 h or 1-y storage in artificial saliva...
May 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/28484394/mitochondrial-dna-mutations-in-grade-ii-and-iii-glioma-cell-lines-are-associated-with-significant-mitochondrial-dysfunction-and-higher-oxidative-stress
#7
Bee Hong Soon, Nor Azian Abdul Murad, Sue-Mian Then, Azizi Abu Bakar, Farizal Fadzil, Jegan Thanabalan, Mohd S Mohd Haspani, Charng Jeng Toh, Azmi Mohd Tamil, Roslan Harun, Wan Z Wan Ngah, Rahman Jamal
The role of mitochondria in tumorigenesis has regained much attention as it could dysregulate cellular energetics, oxidative stress and apoptosis. However, the role of mitochondria in different grade gliomasis still unknown. This study aimed to identify mitochondrial DNA (mtDNA) sequence variations that could possibly affect the mitochondrial functions and also the oxidative stress status. Three different grades of human glioma cell lines and a normal human astrocyte cell line were cultured in-vitro and tested for oxidative stress biomarkers...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28483661/in-vivo-two-photon-imaging-of-retina-in-rabbits-and-rats
#8
Gopal Swamy Jayabalan, Yi-Kai Wu, Josef F Bille, Samuel Kim, Xiao Wen Mao, Howard V Gimbel, Michael E Rauser, Joseph T Fan
The purpose of this study was to evaluate the retina using near-infrared (NIR) two-photon scanning laser ophthalmoscopy. New Zealand white rabbits, albino rats, and brown Norway rats were used in this study. An autofluorescence image of the retina, including the retinal cells and its associated vasculatures was obtained by a real-time scan using the ophthalmoscope. Furthermore, the retinal vessels, nerve fiber layers and the non-pigmented retina were recorded with two-photon fluorescein angiography (FA); and the choroidal vasculatures were recorded using two-photon indocyanine green angiography (ICGA)...
May 5, 2017: Experimental Eye Research
https://www.readbyqxmd.com/read/28468342/performance-of-ingan-based-green-laser-diodes-improved-by-using-an-asymmetric-ingan-ingan-multi-quantum-well-active-region
#9
J Yang, D G Zhao, D S Jiang, X Li, F Liang, P Chen, J J Zhu, Z S Liu, S T Liu, L Q Zhang, M Li
Series of green laser diodes (LDs) with different (In)GaN barrier layers are investigated. It is found that the optical confinement factor of multi-quantum well (MQW) always increases with increasing indium content of InGaN barrier layer, which results in a decrease of threshold current when indium content of InGaN barrier layer increases from 0 to 5%. However, when a high In content InGaN barrier is used (> 5%), both threshold current and slop efficiency of LDs deteriorate. It may be attributed to the waste of carriers in the potential well at the interface between the last barrier (LB) and the upper waveguide (UWG) layers, which is induced by the piezoelectric polarization effect in high In content InGaN LB layer...
May 1, 2017: Optics Express
https://www.readbyqxmd.com/read/28465571/upconversion-modulation-through-pulsed-laser-excitation-for-anti-counterfeiting
#10
Yingdong Han, Hongyu Li, Yangbo Wang, Yue Pan, Ling Huang, Feng Song, Wei Huang
Lanthanide-doped upconversion nanomaterials are emerging as promising candidates in optoelectronics, volumetric display, anti-counterfeiting as well as biological imaging and therapy. Typical modulations of upconversion through chemical methods, such as controlling phase, composition, morphology and size enable us to rationally manipulate emission profiles and lifetimes of lanthanide ions by using continuous-wave laser excitation. Here we demonstrate that under pulsed laser excitation the emission color of NaYF4:Er/Tm (2/0...
May 2, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28457467/isolation-and-characterisation-of-nanoparticles-from-tef-and-maize-starch-modified-with-stearic-acid
#11
Wokadala O Cuthbert, Suprakas S Ray, Naushad M Emmambux
Nanoparticles were isolated from tef and maize starch modified with added stearic acid after pasting at 90°C for 130min. This was followed by thermo-stable alpha-amylase hydrolysis of the paste. The resultant residues were then characterized using X-ray diffraction (XRD), differential scanning calorimetry (DSC), dynamic laser scattering particle size distribution (DLPSD), atomic force microscopy (AFM) and high-resolution transmission electron microscopy (HRTEM). XRD and DSC showed that the isolated residues consisted of amylose-lipid complexes...
July 15, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28454130/directly-modulated-green-light-diode-pumped-solid-state-laser-for-underwater-wireless-optical-communication
#12
Jing Xu, Meiwei Kong, Aobo Lin, Yuhang Song, Jun Han, Zhiwei Xu, Bo Wu, Shiming Gao, Ning Deng
It is widely known that a diode-pumped solid-state laser (DPSSL) has very limited modulation bandwidth. Recently, we directed our attention toward the opportunities for directly modulating a DPSSL to generate high-speed green-light signals, with high power and superior beam quality, which are highly desirable in underwater wireless optical communication. The constraint imposed by the limited modulation bandwidth of a DPSSL is circumvented with the strategy of orthogonal frequency-division multiplexing and power loading...
May 1, 2017: Optics Letters
https://www.readbyqxmd.com/read/28451353/highly-specific-noninvasive-photoacoustic-and-positron-emission-tomography-of-brain-plaque-with-functionalized-croconium-dye-labeled-by-a-radiotracer
#13
Yajing Liu, Yanping Yang, Mingjian Sun, Mengchao Cui, Ying Fu, Yu Lin, Zijing Li, Liming Nie
Highly-efficient targeting probes are desirable for disease diagnosis and functional imaging. However, most of the current near-infrared (NIR) probes suffer from low signal conversion, insufficient photostability, poor probe specificity, and limited functions. Herein, an NIR ultrahigh absorbing croconium dye for amyloid (CDA) was designed and synthesized to specifically bind to cerebrovascular amyloid without antibody linkage. This unique CDA is able to strongly bind the hydrophobic channels of amyloid beta (Aβ) fiber with a very strong binding energy of -9...
April 1, 2017: Chemical Science
https://www.readbyqxmd.com/read/28450261/monitoring-gene-expression-of-rcpa-from-aggregatibacter-actinomycetemcomitans-versus-antimicrobial-photodynamic-therapy-by-relative-quantitative-real-time-pcr
#14
Maryam Pourhajibagher, Nasim Chiniforush, Sima Shahabi, Shaghayegh Sobhani, Mohammad Moein Monzavi, Abbas Monzavi, Abbas Bahador
BACKGROUND: Aggregatibacter actinomycetemcomitans is an important pathogen that is frequently found in various infections, particularly aggressive periodontitis. In this study, we described the outcome of the expression level of A. actinomycetemcomitans virulence factor following treatment by antimicrobial photodynamic therapy (aPDT) with indocyanine green (ICG) as a photosensitizing agent. MATERIALS AND METHODS: To determine the aPDT effect on the cell-surviving assay and expression ratio of the rcpA gene in A...
April 24, 2017: Photodiagnosis and Photodynamic Therapy
https://www.readbyqxmd.com/read/28448021/detection-of-ligand-activated-g-protein-coupled-receptor-internalization-by-confocal-microscopy
#15
Jingwen Yang, Yunjun Yan, Xiaowei Xiang, Yuchao Xu, Naiming Zhou, Tianming Wang
Confocal laser scanning microscopy (CLSM) is an optical imaging technique for high-contrast imaging. It is a powerful approach to visualize fluorescent fusion proteins, such as green fluorescent protein (GFP), to determine their expression, localization, and function. The subcellular localization of target proteins is important for identification, characterization, and functional analyses. Internalization is one of the predominant mechanisms controlling G protein-coupled receptor (GPCR) signaling to ensure the appropriate cellular responses to stimuli...
April 9, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28447987/live-cell-imaging-and-3d-analysis-of-angiotensin-receptor-type-1a-trafficking-in-transfected-human-embryonic-kidney-cells-using-confocal-microscopy
#16
Parnika Kadam, Ryan McAllister, Jeffrey S Urbach, Kathryn Sandberg, Susette C Mueller
Live-cell imaging is used to simultaneously capture time-lapse images of angiotensin type 1a receptors (AT1aR) and intracellular compartments in transfected human embryonic kidney-293 (HEK) cells following stimulation with angiotensin II (Ang II). HEK cells are transiently transfected with plasmid DNA containing AT1aR tagged with enhanced green fluorescent protein (EGFP). Lysosomes are identified with a red fluorescent dye. Live-cell images are captured on a laser scanning confocal microscope after Ang II stimulation and analyzed by software in three dimensions (3D, voxels) over time...
March 27, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/28441978/comparing-the-consistency-of-atom-probe-tomography-measurements-of-small-scale-segregation-and-clustering-between-the-leap-3000-and-leap-5000-instruments
#17
Tomas L Martin, Andrew J London, Benjamin Jenkins, Sarah E Hopkin, James O Douglas, Paul D Styman, Paul A J Bagot, Michael P Moody
The local electrode atom probe (LEAP) has become the primary instrument used for atom probe tomography measurements. Recent advances in detector and laser design, together with updated hit detection algorithms, have been incorporated into the latest LEAP 5000 instrument, but the implications of these changes on measurements, particularly the size and chemistry of small clusters and elemental segregations, have not been explored. In this study, we compare data sets from a variety of materials with small-scale chemical heterogeneity using both a LEAP 3000 instrument with 37% detector efficiency and a 532-nm green laser and a new LEAP 5000 instrument with a manufacturer estimated increase to 52% detector efficiency, and a 355-nm ultraviolet laser...
April 2017: Microscopy and Microanalysis
https://www.readbyqxmd.com/read/28441615/near-infrared-light-stimuli-responsive-synergistic-therapy-nanoplatforms-based-on-the-coordination-of-tellurium-containing-block-polymer-and-cisplatin-for-cancer-treatment
#18
Feng Li, Tianyu Li, Wei Cao, Lu Wang, Huaping Xu
Cisplatin (CDDP) has received worldwide approval for clinical use in the past decades. However, its development in cancer chemotherapy was overshadowed by severe side effects and drug resistance. Herein, we developed a CDDP drug delivery system with high encapsulation efficiency and near-infrared light stimuli-responsive drug release properties based on the coordination of novel tellurium-containing block polymer (PEG-PUTe-PEG) and CDDP. The nanocarriers made from PEG-PUTe-PEG were loaded with CDDP and indocyanine green (ICG) simultaneously...
April 17, 2017: Biomaterials
https://www.readbyqxmd.com/read/28439814/imaging-the-accumulated-intracellular-microalgal-lipids-as-a-response-to-temperature-stress
#19
Khaled N M Elsayed, Tatiana A Kolesnikova, Anja Noke, Gerd Klöck
Over the last few decades, many scientists considered microalgae as promising actors for future biofuels because of the high lipid productivity inside their cells. Moreover, much attention has been paid to algal lipids as they can be used in biodiesel production. In this study, we optimized the different suitable conditions such as incubation time, incubation temperature, Dimethylesulfoxide and Nile red concentrations of the lipophilic fluorescence dye Nile red as an excellent and fast vital stain to detect and quantify intracellular lipids...
May 2017: 3 Biotech
https://www.readbyqxmd.com/read/28438509/effect-of-photodynamic-therapy-based-on-indocyanine-green-on-expression-of-apoptosis-related-genes-in-human-gingival-fibroblast-cells
#20
Samira Gharesi, Maryam Pourhajibagher, Nasim Chiniforush, Reza Raoofian, Mehrdad Hashemi, Sima Shahabi, Abbas Bahador
BACKGROUND: Periodontal diseases refer to inflammation of the gingiva, induction of apoptosis in human gingival fibroblast cells, destruction of the surrounding tissues, and early bone loss resulting in infections due to the pathogenic activity of the microorganisms and the host immune inflammatory responses. Recent investigations have suggested that antimicrobial photodynamic therapy (aPDT) can be an adjunct treatment therapy for periodontal infections. AIM: To prove the lack of side effects of PDT on periodontal tissues, we investigated the expression of BAX and BCL-2 genes that are involved in apoptosis after the PDT on human gingival fibroblast (HGF) cells...
April 21, 2017: Photodiagnosis and Photodynamic Therapy
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