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Madina Makhmutova, Tao Liang, Herbert Gaisano, Alejandro Caicedo, Joana Almaça
Insulin secretion plays a central role in glucose homeostasis under normal physiological conditions as well as in disease. Current approaches to study insulin granule exocytosis either use electrophysiology or microscopy coupled to the expression of fluorescent reporters. However most of these techniques have been optimized for clonal cell lines or require dissociating pancreatic islets. In contrast, the method presented here allows for real time visualization of insulin granule exocytosis in intact pancreatic islets...
September 13, 2017: Journal of Visualized Experiments: JoVE
Tim Kohlmann, Robert Naumann, Christoph Kerzig, Martin Goez
The hydrated electron represents a "super-reductant" in water, providing 2.9 eV of reductive power, which suffices to decompose nonactivated aliphatic halides. We show that 3-amino-perylene in SDS micelles, when combined with the bioavailable ascorbate as an extramicellar sacrificial donor, sustainably produces hydrated electrons through photoredox catalysis with green light, from a metal-free system, and at near-physiological pH. Photoionization of the amine with a 532 nm laser yields an extremely long-lived radical cation as the by-product, and a subsequent reaction of the latter with the sacrificial donor across the micelle/water interface regenerates the catalyst...
September 19, 2017: Photochemical & Photobiological Sciences
Erika M du Plessis, Sarasha Govender, Bala Pillay, Lise Korsten
Knowledge of the microbiological quality and prevalence of antibiotic resistance and virulence genes in bacterial isolates from leafy green vegetables supplied by formal suppliers (retailers) and informal suppliers (street vendors) in South Africa is limited. Because leafy vegetables have been implicated in foodborne disease outbreaks worldwide, 180 cabbage and spinach samples were collected from three major retailers and nine street vendors in Johannesburg, South Africa. Escherichia coli and coliforms were enumerated using Petrifilm plates...
September 18, 2017: Journal of Food Protection
Mahmoud O Jaroudi, Carole G Cherfan, Shady T Awwad
A 27-year-old woman had microkeratome-assisted laser in situ keratomileusis (LASIK) for moderate bilateral hyperopia. The preoperative ophthalmologic examination was unremarkable except for minimal lissamine green staining bilaterally on the nasal conjunctiva. On the first postoperative day, the uncorrected distance visual acuity (UDVA) was 20/20 bilaterally and the corneas were clear. Four days later, the patient presented with mild blur in both eyes. Examination showed diffuse bilateral epithelial cysts encompassing the central 6...
August 2017: Journal of Cataract and Refractive Surgery
J R Harwell, G L Whitworth, G A Turnbull, I D W Samuel
A visible perovskite distributed feedback laser is fabricated for the first time. Through the use of nanocrystal pinning, highly luminescent methylammonium lead bromide films are used to produce stable lasers emitting at 550 nm, with a low threshold of 6 µJcm(-2). The lasers were able to support multiple polarisations, and could be switched between transverse magnetic and transverse electric mode operation through simple tuning of the distributed feedback grating period.
September 15, 2017: Scientific Reports
Ti Wang, Lu Zhu, Yinbo Peng, Nianci Shen, Yan Yu, Min Yao, Jingyin Zhu
PURPOSE: To compare the efficacy of photochemical-induced tissue cross-linking (PCL), utilizing Rose Bengal (RB) and 532 nm green light irradiation (RB-PCL), with standard sutures for closure of penetrating corneal incision in porcine cadaver eyes. METHODS: A full-thickness penetrating incision, 3 mm in length parallel to the limbus and perpendicular to the corneal surface, was made in the enucleated porcine cornea. Photochemical cross-linking was performed with tropical RB application and irradiation of 532 nm green light (0...
September 14, 2017: Current Eye Research
Anita Kumari, Manisha Mondal, Vineet Kumar Rai, Satyendra Narayan Singh
Ho3+/Yb3+/Tm3+/Li+:Gd2(MoO4)3 nanophosphors successfully synthesized via solid state reaction method have been structurally and optically characterized. Under 980 nm diode laser excitation the nanophosphors emit intense blue, green, red and NIR emissions peaking at ~ 476 nm, ~ 543 nm, ~ 646 nm and ~ 798 nm corresponding to the 1G4→3H6 (Tm3+), 5F4, 5S2→5I8 (Ho3+), 5F5 →5I8 (Ho3+) and 3H4→3H6 (Tm3+) transitions respectively. The UC emission intensity enhancement in the Ho3+-Yb3+-Tm3+-Li+:Gd2(MoO4)3 nanophosphors for the green band is found to be ~ 367, ~ 50 and ~ 9 times compared to the singly Ho3+ doped, Ho3+-Yb3+ co-doped and Ho3+-Yb3+-Tm3+ tri-doped Gd2(MoO4)3 nanophosphors...
September 13, 2017: Methods and Applications in Fluorescence
Bo Li, Lan Jiang, Xin Li, Peng Ran, Pei Zuo, Andong Wang, Liangti Qu, Yang Zhao, Zhihua Cheng, Yongfeng Lu
Zero-dimensional MoS2 quantum dots (QDs) possess distinct physical and chemical properties, which have garnered them considerable attention and facilitates their use in a broad range of applications. In this study, we prepared monolayer MoS2 QDs using temporally shaped femtosecond laser ablation of bulk MoS2 targets in water. The morphology, crystal structures, chemical, and optical properties of the MoS2 QDs were characterized by transmission electron microscopy, X-ray diffraction, Raman spectroscopy, X-ray photoelectron spectroscopy, UV-vis absorption spectra, and photoluminescence spectra...
September 11, 2017: Scientific Reports
Purushothaman Kuppan, Swaminathan Sethuraman, Uma Maheswari Krishnan
Esophagus is a complex, hollow organ consisting of epithelial cells in the inner mucosal layer and smooth muscle cells in the outer muscle layer. In the present study, we have evaluated the in vitro co-culture of epithelial cells and smooth muscle cells on the aligned nanofibrous scaffold made of PHBV, PHBV-gelatin, PCL and PCL-gelatin developed through electrospinning using rotating drum collector. Epithelial cells were labeled with cell tracker green while the smooth muscle cells were labeled with cell tracker red...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Ning Yuan, Hong-Xue Sun, Dan-Dan Ju, Da-Yu Liu, Zi-Bo Zhang, Wing-Han Wong, Feng Song, Dao-Yin Yu, Edwin Yue-Bun Pun, De-Long Zhang
We developed a highly efficient optical thermometer based on intensity ratio of upconversion green fluorescence of Er(3+)/Yb(3+)-codoped NaYF4 microcrystals. The sensor consists simply of a 980nm laser diode, one narrow-band interference filter, two lenses, one Si-photocell and one multimeter, while being without use of spectrometer and additional electronics. The device not only has a simple, compact structure (hence a low cost), but also displays highly efficient sensing performance, characterized by large signal-to-noise ratio due to strong fluorescence intensity, high thermal resolution and sensitivity, which have the values 1...
December 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Aroon Teerakapong, Teerasak Damrongrungruang, Sajee Sattayut, Noppawan Phumala Morales, Surada Tantananugool
BACKGROUND: The purpose of this in vitro study was to evaluate the efficacy of erythrosine and cyanidin-3-glucoside as photosensitizers in PDT for the elimination of Porphyromonas gingivalis (P. gingivalis) biofilms. METHODS: P. gingivalis biofilms were prepared from a chronic periodontitis subject. Erythrosine and cyanidin-3-glucoside were prepared and randomly allocated as follows: 110, 220, 330, and 440μM erythrosine; 101, 202, 303, and 404μM anthocyanin; and 440μM erythrosine+404μM cyanidin-3-glucoside...
September 5, 2017: Photodiagnosis and Photodynamic Therapy
Rita Mendes, Pedro Pedrosa, João C Lima, Alexandra R Fernandes, Pedro V Baptista
Photothermal Therapy (PTT) impact in cancer therapy has been increasing due to the enhanced photothermal capabilities of a new generation of nanoscale photothermal agents. Among these nanoscale agents, gold nanoshells and nanorods have demonstrated optimal properties for translation of near infra-red radiation into heat at the site of interest. However, smaller spherical gold nanoparticles (AuNPs) are easier to produce, less toxic and show improved photoconversion capability that may profit from the irradiation in the visible via standard surgical green lasers...
September 7, 2017: Scientific Reports
Kathleen Lange, Christian Lautenschläger, Maria Wallert, Stefan Lorkowski, Andreas Stallmach, Alexander Schiller
We here describe a new approach to visualise nitric oxide (NO) in living macrophages by fluorescent NO-sensitive microspheres based on poly(lactic-co-glycolic acid) (PLGA). PLGA microspheres loaded with NO550 dye were prepared through a modified solvent-evaporation method. Microparticles were characterized by a mean hydrodynamic diameter of 3000 nm, zeta potential of -26.000 ± 0.351 mV and a PDI of 0.828 ± 0.298. Under abiotic conditions, NO release was triggered through UV radiation (254 nm) of 10 mM sodium nitroprusside dehydrate (SNP)...
2017: Beilstein Journal of Nanotechnology
Damian S Nakonieczny, Zbigniew K Paszenda, Marcin Basiaga, Tomasz Radko, Sabina Drewniak, Jacek Podwórny, Wojciech Bogacz
PURPOSE: High purity, fine crystalline, degradation-free at low temperature powders have attracted special interest in CAD/CAM prosthetic dentistry full ceramic restorations. This study reports the preparation and characterisation of zirconia-ceria (0.9 ZrO2 0.1 CeO2) powders. Materials were obtained from zirconium-n-alkoxide and cerium nitrate hexahydrate in the pH 2-4 and 8-10. METHODS: Zirconia- ceria powders were obtained with the sol-gel method in a humid-free environment...
2017: Acta of Bioengineering and Biomechanics
Chen Wang, Liangge Xu, Jiating Xu, Dan Yang, Bin Liu, Shili Gai, Fei He, Piaoping Yang
Core-shell nanostructures consisting of plasmonic materials and lanthanide-doped upconversion nanoparticles (UCNPs) show promising applications in theranostics including bio-imaging, diagnosis and therapy. However, some challenges still remain in the synthetic control because of the non-coordination between energy transfer and photothermal therapy (PTT). Herein, we developed a novel type of thermal-fluorescent core-shell hybrid nanocomposite incorporating rare-earth Yb(3+) and Er(3+) ion doped GdOF as the shell and gold nanorods (GNRs) as the core, creating upconversion nanorods (UCNRs) of GNRs@GdOF:Yb(3+),Er(3+)...
September 28, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
Chaoqun You, Mingxin Wang, Hongshuai Wu, Peijing An, Mengmeng Pan, Yanghui Luo, Baiwang Sun
Synergistic therapy has caused increasing interest in recent treatment of cancer owing to its preferable therapeutic efficiency to most single antineoplastic protocol. Herein, we design a co-delivery two drugs nanosystem based on biodegradable liposomes, loading cisplatin, Indocyanine green (ICG), and CJM126 coupled with cholesterol derivative (CJM-Chol) for the purpose of synergistic therapy. The obtained nanoparticles showed a uniform diameter of 103.8nm and a favorable morphology. The investigation on near infrared radiated (NIR) responsive release showed that NIR mediated photothermal conversion induced a controllable drug release from liposomes...
November 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Hui-Quan Wang, Peng-Fei Hu, Yu Zheng, Zhe Zhao, Bin Zheng, Jin Chang, Han-Jie Wang, Jin-Hai Wang
Photothermal therapy (PTT) has drawn tremendous attention because of its high therapeutic efficiency in targeting cells while minimizing the damage to normal tissues and organs. Tungsten oxide (W18O49, WO) plays a pivotal role in PTT development and its use in PTT systems has been extensively studied. However, it is difficult to control morphology of WO through conventional hydrothermal method. Which make its related researches have been limited up to now. In this study, we describe the construction and effects on tumor of a novel nanoplatform based on WO and indocyanine green (ICG) loaded in mesoporous silica nanoparticles (MSN) for dual-modal PTT and near-infrared imaging...
November 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
Sushil Kumar Ranjan, Abhishek Kumar Soni, Vineet Kumar Rai
Near infrared (NIR) to visible frequency upconversion emission studies in Er(3+)-Eu(3+)/Er(3+)-Eu(3+)-Yb(3+) co-doped/tri-doped Gd2O3 phosphors prepared by the co-precipitation technique have been explored under 980 nm laser diode radiation. The developed phosphors were characterized with the help of XRD, FE-SEM and FTIR analysis. No upconversion (UC) emission was found in the Eu(3+)-doped Gd2O3 phosphor. UC emission from Eu(3+) ions along with Er(3+) ions was observed in Er(3+)-Eu(3+) and Er(3+)-Eu(3+)-Yb(3+) co-doped/tri-doped phosphors...
September 1, 2017: Methods and Applications in Fluorescence
Bo Cheng, Xiaofu Qiu, Huanhui Li, Guosheng Yang
PURPOSE: Laser therapy provides an alternative option for treating non-muscle-invasive bladder cancer (NMIBC). However, the clinical evidence for potassium-titanyl-phosphate (KTP) laser en bloc resection is still limited. Here, we investigated the efficacy and safety of the 120-W front-firing KTP laser for the treatment of NMIBC. METHODS: A total of 64 patients with NMIBC treated with either a 120-W front-firing KTP-photoselective vapo-enucleation of the bladder tumor (PVEBT, n=34) or transurethral resection of the bladder tumor (TURBT, n=30) were included...
2017: Therapeutics and Clinical Risk Management
Eugene G Novikov, Victor V Skakun, Jan Willem Borst, Antonie J W G Visser
The Maximum Entropy Method (MEM) was used for the analysis of polarized fluorescence decays of enhanced green fluorescent protein (EGFP) in buffered water/glycerol mixtures, obtained with time-correlated single-photon counting (Visser et al., Methods Appl. Fluoresc. 4 (2016) 035002). To this end, we used a general-purpose software module of MEM that was earlier developed to analyze (complex) laser photolysis kinetics of ligand rebinding reactions in oxygen binding proteins. We demonstrate that the MEM software provides reliable results and is easy to use for the analysis of both total fluorescence decay and fluorescence anisotropy decay of aqueous solutions of EGFP...
August 31, 2017: Methods and Applications in Fluorescence
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