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https://www.readbyqxmd.com/read/27933363/spatially-resolved-metabolic-distribution-for-unraveling-the-physiological-change-and-responses-in-tomato-fruit-using-matrix-assisted-laser-desorption-ionization-mass-spectrometry-imaging-maldi-msi
#1
Junya Nakamura, Tomomi Morikawa-Ichinose, Yoshinori Fujimura, Eisuke Hayakawa, Katsutoshi Takahashi, Takanori Ishii, Daisuke Miura, Hiroyuki Wariishi
Information on spatiotemporal metabolic behavior is indispensable for a precise understanding of physiological changes and responses, including those of ripening processes and wounding stress, in fruit, but such information is still limited. Here, we visualized the spatial distribution of metabolites within tissue sections of tomato (Solanum lycopersicum L.) fruit using a matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) technique combined with a matrix sublimation/recrystallization method...
December 8, 2016: Analytical and Bioanalytical Chemistry
https://www.readbyqxmd.com/read/27916858/three-dimensional-continuous-displacement-measurement-with-temporal-speckle-pattern-interferometry
#2
Jie Qin, Zhan Gao, Xu Wang, Shanwei Yang
A speckle interferometer which can measure whole field three-dimensional displacements continuously and dynamically has been built. Three different wavelength lasers are used to produce the speckle interferograms of the two in-plane displacements (displacements in the x- and y-direction) and one out-of-plane displacement (displacement in the z-direction), respectively. One color CCD camera is employed to collect these mixed speckle interferograms simultaneously. The mixed interferograms are separated by the Red, Green and Blue channels of the color CCD camera, and then are processed by the wavelet transform technique to extract the phase information of the measured object...
November 29, 2016: Sensors
https://www.readbyqxmd.com/read/27916691/therapeutic-effects-of-gold-nanoparticles-synthesized-using-musa-paradisiaca-peel-extract-against-multiple-antibiotic-resistant-enterococcus-faecalis-biofilms-and-human-lung-cancer-cells-a549
#3
S Vijayakumar, B Vaseeharan, B Malaikozhundan, N Gopi, P Ekambaram, R Pachaiappan, P Velusamy, K Murugan, G Benelli, R Suresh Kumar, M Suriyanarayanamoorthy
Botanical-mediated synthesis of nanomaterials is currently emerging as a cheap and eco-friendly nanotechnology, since it does not involve the use of toxic chemicals. In the present study, we focused on the synthesis of gold nanoparticles using the aqueous peel extract of Musa paradisiaca (MPPE-AuNPs) by green approach following a facile and cheap fabrication process. The green synthesized MPPE-AuNPs were bio-physically characterized by UV-Vis spectroscopy, FTIR, XRD, TEM, Zeta potential and EDX. MPPE-AuNPs were crystalline in nature, spherical to triangular in shape, with particle size ranged within 50 nm...
December 1, 2016: Microbial Pathogenesis
https://www.readbyqxmd.com/read/27911432/-eye-lid-hygiene-in-chronic-allergic-blepharoconjunctivitis-patients-before-laser-refractive-surgery
#4
S V Yanchenko, A V Malyshev, S N Sakhnov, N V Fedotova, O Yu Orekhova
AIM: To evaluate the effectiveness of eyelid hygiene in patients with chronic allergic blepharoconjunctivitis (ABC) as a part of their preparation for laser refractive surgery. MATERIAL AND METHODS: The study involved 32 patients (12 males and 20 females aged 25-42 years) with refractive errors, namely, compound myopic astigmatism (23 patients) and hyperopic astigmatism (9 patients) suffering from chronic ABC and secondary dry eye syndrome (DES). All the patients initially received a standard treatment for ABC and DES, that is olopatodin hydrochloride instillations - 1 mg/ml 2 times daily, preservative-free hyaluronic acid preparation - 1 mg/ml 3 times daily, and polyacrylic acid and dexpanthenol gel at night for one month...
2016: Vestnik Oftalmologii
https://www.readbyqxmd.com/read/27906399/unusual-reactivity-of-dithiol-protected-clusters-in-comparison-to-monothiol-protected-clusters-studies-using-ag51-bdt-19-tpp-3-and-ag29-bdt-12-tpp-4
#5
Atanu Ghosh, Debasmita Ghosh, Esma Khatun, Papri Chakraborty, Thalappil Pradeep
We report the synthesis and unique reactivity of a new green dithiol protected cluster (DTPC), Ag51(BDT)19(TPP)3 (BDT and TPP are 1,3-benzenedithiol and triphenylphosphine, respectively). The cluster composition was confirmed by electrospray ionization (ESI) and matrix-assisted laser desorption ionization (MALDI) mass spectrometric studies as well as by other supporting data. Surprisingly, the chemical reactivity between this DTPC and Au25(SR)18 involves only metal ion exchange in Au25(SR)18 without any ligand exchange, while reactions between monothiol protected clusters (MTPCs) show both metal and ligand exchange, an example being the reaction between Ag25DMBT18 and Au25PET18 (where DMBT and PET are 2,4-dimethylbenzenethiol and phenylethanethiol, respectively)...
December 1, 2016: Nanoscale
https://www.readbyqxmd.com/read/27906322/detection-of-gaseous-elemental-mercury-using-a-frequency-doubled-green-diode-laser
#6
Xiutao Lou, Tie Zhang, Hongze Lin, Shiyi Gao, Lianjie Xu, Junnan Wang, Li Wan, Sailing He
We demonstrate a second-harmonic-generation (SHG) based method for the detection of gaseous elemental mercury by using a newly available green diode laser. Multimode ultraviolet radiation at 253.7 nm is generated through a process of SHG. Correlation spectroscopy is introduced into the scheme to guarantee the measurement accuracy. The limit of detection achieved is 0.6 μg/m<sup>3</sup> (0.07 ppb) for 1-m pathlength and 10-s integration time. The measurement accuracy is estimated to be 1.2%. The linear response range is estimated to be 0~60 μg/m<sup>2</sup> (6...
November 28, 2016: Optics Express
https://www.readbyqxmd.com/read/27906234/generation-of-spectral-clusters-in-a-mixture-of-noble-and-raman-active-gases
#7
Pooria Hosseini, Amir Abdolvand, Philip St J Russell
We report a novel scheme for the generation of dense clusters of Raman sidebands. The scheme uses a broadband-guiding hollow-core photonic crystal fiber (HC-PCF) filled with a mixture of H<sub>2</sub>, D<sub>2</sub>, and Xe for efficient interaction between the gas mixture and a green laser pump pulse (532 nm, 1 ns) of only 5 μJ of energy. This results in the generation from noise of more than 135 rovibrational Raman sidebands covering the visible spectral region with an average spacing of only 2...
December 1, 2016: Optics Letters
https://www.readbyqxmd.com/read/27905699/green-light-vaporization-of-the-prostate-pvp-is-it-an-adult-technique
#8
Aldo Brassetti, Cosimo De Nunzio, Nicolas Barry Delongchamps, Cristian Fiori, Francesco Porpiglia, Andrea Tubaro
INTRODUCTION: Intending to overcome Transurethral resection of the prostate (TURP) in terms of safety maintaining its efficacy profile, have led to the introduction of minimally invasive laser therapies to treat men with lower urinary tract symptoms (LUTS) secondary to Benign prostatic obstruction (BPO), each one with its unique properties. Aim of this review was to analyse and summarize all the existing data regarding the 180 W Xcelerated Performance System (XPS) Potoselective Vaporization of the Prostate (PVP)...
December 1, 2016: Minerva Urologica e Nefrologica, the Italian Journal of Urology and Nephrology
https://www.readbyqxmd.com/read/27904270/morphologic-changes-of-zebrafish-melanophore-after-intense-pulsed-light-and-q-switched-nd-yag-laser-irradiation
#9
Hwa Jung Ryu, Ji Min Lee, Hee Won Jang, Hae Chul Park, Im Joo Rhyu, Il-Hwan Kim
BACKGROUND: Recently, the pulse-in-pulse mode of intense pulsed light (IPL) has been used increasingly for the treatment of melasma. OBJECTIVE: To observe the morphologic changes in the melanophore in adult zebrafish after irradiation with conventional and pulse-in-pulse IPL and Q-switched Nd:YAG (QSNY) laser. METHODS: Adult zebrafish were irradiated with conventional and pulse-in-pulse mode of IPL. The conditions for conventional IPL were 3 mJ/cm(2), 560 nm filter, and pulse widths of 7, 20, and 35 msec...
December 2016: Annals of Dermatology
https://www.readbyqxmd.com/read/27897297/facile-one-pot-synthesis-of-fe3o4-chitosan-nanospheres-for-mri-and-fluorescence-imaging-guided-chemo-photothermal-combinational-cancer-therapy
#10
Zhifang Gao, Xijian Liu, Yeying Wang, Guoying Deng, Feng Zhou, Qian Wang, Lijuan Zhang, Jie Lu
Multifunctional nanoplatforms that integrate imaging and chemo-photothermal therapy for highly efficient cancer diagnostics and therapeutics have gained great interest in recent years. Herein, we designed and synthesized biodegradable and biocompatible magnetic chitosan nanospheres (Fe3O4@CS) by a facile one-pot synthesis method. Doxorubicin (DOX, an anticancer drug) and indocyanine green (ICG) can be co-loaded into the Fe3O4@CS nanospheres (Fe3O4@CS-ICG/DOX nanocomposites) by virtue of the mesoporous structure and electrostatic interactions of CS...
November 29, 2016: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/27896017/simple-approach-to-three-color-two-photon-microscopy-by-a-fiber-optic-wavelength-convertor
#11
Kuen-Che Li, Lynn L H Huang, Jhih-Hao Liang, Ming-Che Chan
A simple approach to multi-color two-photon microscopy of the red, green, and blue fluorescent indicators was reported based on an ultra-compact 1.03-μm femtosecond laser and a nonlinear fiber. Inside the nonlinear fiber, the 1.03-μm laser pulses were simultaneously blue-shifted to 0.6~0.8 μm and red-shifted to 1.2~1.4 μm region by the Cherenkov radiation and fiber Raman gain effects. The wavelength-shifted 0.6~0.8 μm and 1.2~1.4 μm radiations were co-propagated with the residual non-converted 1.03-μm pulses inside the same nonlinear fiber to form a fiber-output three-color femtosecond source...
November 1, 2016: Biomedical Optics Express
https://www.readbyqxmd.com/read/27894613/comparative-study-between-thulium-laser-tm-yag-150w-and-greenlight-laser-lbo-nd-yag-120w-for-the-treatment-of-benign-prostatic-hyperpplasia-short-term-efficacy-and-security
#12
J L Palmero-Martí, J Panach-Navarrete, L Valls-González, A Ganau-Ituren, J Miralles-Aguado, A Benedicto-Redón
OBJECTIVE: To compare the results of efficacy and safety of Thulium laser 150W against Greenlight laser 120W in the treatment of short term benign prostatic hyperplasia (12 months after surgery). MATERIAL AND METHODS: This is a retrospective observational study where men who underwent the surgical technique of prostate vaporization over a period of four years in our center are included. The homogeneity of the sample was checked, and postoperative complications (acute urinary retention, reentry, need for transfusion), failures per year of surgery (reoperation, peak flow <15ml/sec, no improvement in comparing the I-PSS), and decreased PSA were compared a year after surgery...
November 25, 2016: Actas Urologicas Españolas
https://www.readbyqxmd.com/read/27892715/incorporation-of-chemotherapeutic-agent-and-photosensitizer-in-a-low-temperature-sensitive-liposome-for-effective-chemo-hyperthermic-anticancer-activity
#13
Hanh Thuy Nguyen, Tuan Hiep Tran, Raj Kumar Thapa, Tung Thanh Pham, Jee-Heon Jeong, Yu Seok Youn, Han-Gon Choi, Chul Soon Yong, Jong Oh Kim
OBJECTIVES: In this study, we combined chemo- and hyperthermia therapy in a low temperature-sensitive liposome (LTSL) for potential cancer treatment. METHODS: Docetaxel (DOC) and indocyanine green (ICG) as a therapeutic agent and photosensitizer, respectively, were incorporated in a low temperature-sensitive liposome (LTSL/DI). Nanoparticles were evaluated for the physicochemical characterizations, in vitro uptake and cytotoxicity, and furthermore in vivo anticancer activity...
November 28, 2016: Expert Opinion on Drug Delivery
https://www.readbyqxmd.com/read/27892489/laser-light-induced-transformation-of-molybdenum-disulphide-based-nanoplatelet-arrays
#14
Arūnas Jagminas, Gediminas Niaura, Rokas Žalnėravičius, Romualdas Trusovas, Gediminas Račiukaitis, Vitalija Jasulaitiene
One-pot hydrothermal synthesis of MoS2 nanoplatelet arrays on various substrates is perhaps the most promising approach to fabricate efficient electrocatalysts for hydrogen evolution reaction. However, the main challenges in this synthesis remain the purity and crystallinity of MoS2. In this study, we show for the first time that irradiation of amorphous, defect-rich MoS2 nanoplatelets with a green nanosecond laser at a proper irradiation dose, ca ≤0.35 J cm(-2), can significantly improve the crystallinity and purity of MoS2 nanoplatelets...
November 28, 2016: Scientific Reports
https://www.readbyqxmd.com/read/27874198/crataeva-nurvala-nanoparticles-inhibit-virulence-factors-and-biofilm-formation-in-clinical-isolates-of-pseudomonas-aeruginosa
#15
Syed Ghazanfar Ali, Mohammad Azam Ansari, Haris M Khan, Mohammad Jalal, Abbas Ali Mahdi, Swaranjit Singh Cameotra
Green synthesized nanoparticles have gained great attention due to their non-toxic and non-hazardous nature. In the present study, bark extract of the medicinal plant in Ayurveda Crataeva nurvala (Buch-Ham) (CN) was chosen for the biosynthesis of silver nanoparticles (AgNPs). These NPs were characterized by Ultra violet visible spectroscopy, Fourier Transform Infra Red, Atomic Force Microscopy, and Transmission Electron Microscopy (TEM). The average particle size of green synthesized CN-AgNPs was 15.2 ± 1...
November 22, 2016: Journal of Basic Microbiology
https://www.readbyqxmd.com/read/27864931/spatial-distribution-of-synapses-on-tyrosine-hydroxylase-expressing-juxtaglomerular-cells-in-the-mouse-olfactory-glomerulus
#16
Emi Kiyokage, Kazuto Kobayashi, Kazunori Toida
Olfactory sensory axons converge in specific glomeruli where they form excitatory synapses onto dendrites of mitral/tufted (M/T) and juxtaglomerular (JG) cells, including periglomerular (PG), external tufted (ET), and superficial-short axon cells. JG cells consist of heterogeneous subpopulations with different neurochemical, physiological, and morphological properties. Among JG cells, previous electron microscopic (EM) studies showed that the majority of synaptic inputs to tyrosine hydroxylase (TH)-immunoreactive neurons were asymmetrical synapses from olfactory nerve (ON) terminals...
November 16, 2016: Journal of Comparative Neurology
https://www.readbyqxmd.com/read/27861873/notch-signaling-pathway-regulates-angiogenesis-via-endothelial-cell-in-3d-co-culture-model
#17
Dan Zhao, Changyue Xue, Shiyu Lin, Sirong Shi, Qianshun Li, Mengting Liu, Xiaoxiao Cai, Yunfeng Lin
This study aimed to investigate the role of Notch signaling pathway for angiogenesis in a three-dimensional (3D) collagen gel model with co-culture of adipose-derived stromal cells (ASCs) and endothelial cells (ECs). A 3D collagen gel model was established in vitro by implanting both ASCs from green fluorescent protein-labeled mouse and ECs from red fluorescent protein-labeled mouse, and the phenomena of angiogenesis with Notch signaling inducer Jagged1, inhibitor DAPT and PBS, respectively were observed by confocal laser scanning microscopy...
November 10, 2016: Journal of Cellular Physiology
https://www.readbyqxmd.com/read/27859390/stone-tissue-differentiation-for-holmium-laser-lithotripsy-using-autofluorescence-clinical-proof-of-concept-study
#18
Birgit Lange, Dieter Jocham, Ralf Brinkmann, Jens Cordes
BACKGROUND AND OBJECTIVES: Holmium laser lithotripsy is the gold standard for intracorporeal fragmentation of urinary calculi. Usually, a visible beam is superimposed on the IR treatment laser as an aiming beam to guide the surgeon. In vitro tests showed that this aiming beam (532 nm, power <1 mW) excites strong fluorescence on human calculi. Tissue, in contrast, emitted much weaker fluorescence. If this is verified in vivo, the fluorescence signal induced by the aiming beam could be used to implement a feedback loop, preventing the Holmium laser being fired on tissue...
November 12, 2016: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/27857751/rhesus-monkey-neural-stem-cell-transplantation-promotes-neural-regeneration-in-rats-with-hippocampal-lesions
#19
Li-Juan Ye, Hui Bian, Yao-Dong Fan, Zheng-Bo Wang, Hua-Lin Yu, Yuan-Ye Ma, Feng Chen
Rhesus monkey neural stem cells are capable of differentiating into neurons and glial cells. Therefore, neural stem cell transplantation can be used to promote functional recovery of the nervous system. Rhesus monkey neural stem cells (1 × 10(5) cells/μL) were injected into bilateral hippocampi of rats with hippocampal lesions. Confocal laser scanning microscopy demonstrated that green fluorescent protein-labeled transplanted cells survived and grew well. Transplanted cells were detected at the lesion site, but also in the nerve fiber-rich region of the cerebral cortex and corpus callosum...
September 2016: Neural Regeneration Research
https://www.readbyqxmd.com/read/27857366/laser-power-density-dependent-energy-transfer-between-tm-sup-3-sup-and-tb-sup-3-sup-tunable-upconversion-emissions-in-nayf-sub-4-sub-tm-sup-3-sup-tb-sup-3-sup-yb-sup-3-sup-microcrystals
#20
Xiaojie Xue, Makhin Thitsa, Tonglei Cheng, Weiqing Gao, Dinghuan Deng, Takenobu Suzuki, Yasutake Ohishi
Energy transfer between Tm<sup>3+</sup> and Tb<sup>3+</sup> dependent on the power density of pump laser was investigated in NaYF<sub>4</sub>: Tb<sup>3+</sup>,Tm<sup>3+</sup>,Yb<sup>3+</sup> microcrystals. Under the excitation of a 976-nm near-infrared laser at various power densities, Tb<sup>3+</sup>-Tm<sup>3+</sup>-Yb<sup>3+</sup> doped samples exhibited intense visible emissions with tunable color between green and blue...
November 14, 2016: Optics Express
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