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https://www.readbyqxmd.com/read/28324506/induction-of-site-specific-oxidative-damage-at-telomeres-by-killerred-fused-shelretin-proteins
#1
Rong Tan, Li Lan
Chronic oxidative stress is the major endogenous metabolic stress and contributes directly to telomere shortening and senescence. Understanding the dysfunction of telomeres under oxidative stress will greatly facilitate healthy aging and advance the treatment of aging-related diseases. Here, we describe the KR-TEL (KillerRed induced DNA damage at telomeres) system that induces site-specific oxidative damage at telomeres. We have developed the KR-TEL system by fusing killerred with the shelterin component TRF1 (KR-TRF1) or other shelterin proteins...
2017: Methods in Molecular Biology
https://www.readbyqxmd.com/read/28306236/hydrophobic-ir-780-dye-encapsulated-in-crgd-conjugated-solid-lipid-nanoparticles-for-nir-imaging-guided-photothermal-therapy
#2
Ye Kuang, Kunchi Zhang, Yi Cao, Xing Chen, Kewei Wang, Min Liu, Renjun Pei
It is highly demanded to improve the accumulation of NIR theranostic agents in tumor region, which is favorable to the effective phototherapy. IR-780 iodide, a near-infrared (NIR) fluorescence dye, possesses higher and more stable fluorescence intensity than clinically applied dye indocyanine green (ICG), and can be utilized in imaging-guided photothermal therapy with laser irradiation. However, lipophilicity, short circulation time and lack of targeting specificity limit its applications in cancer imaging and therapy...
March 17, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/28302159/a-data-driven-approach-links-microglia-to-pathology-and-prognosis-in-amyotrophic-lateral-sclerosis
#3
Johnathan Cooper-Knock, Claire Green, Gabriel Altschuler, Wenbin Wei, Joanna J Bury, Paul R Heath, Matthew Wyles, Catherine Gelsthorpe, J Robin Highley, Alejandro Lorente-Pons, Tim Beck, Kathryn Doyle, Karel Otero, Bryan Traynor, Janine Kirby, Pamela J Shaw, Winston Hide
Amyotrophic lateral sclerosis (ALS) is a devastating neurodegenerative disease that lacks a predictive and broadly applicable biomarker. Continued focus on mutation-specific upstream mechanisms has yet to predict disease progression in the clinic. Utilising cellular pathology common to the majority of ALS patients, we implemented an objective transcriptome-driven approach to develop noninvasive prognostic biomarkers for disease progression. Genes expressed in laser captured motor neurons in direct correlation (Spearman rank correlation, p < 0...
March 16, 2017: Acta Neuropathologica Communications
https://www.readbyqxmd.com/read/28300263/size-specific-emission-in-peptide-capped-gold-quantum-clusters-with-tunable-photoswitching-behavior
#4
Abhishek Baral, Kingshuk Basu, Sirshendu Ghosh, Kalishankar Bhattacharyya, Subhasish Roy, Ayan Datta, Arindam Banerjee
Three different types of fluorescent gold clusters (namely blue, green and red emitting) have been prepared from a gold precursor (chloroauric acid) under moderate conditions in aqueous medium. A cysteine containing dipeptide has been used for the formation of these quantum clusters as this peptide molecule contains a thiol group in the side chain to cap these nascently formed clusters and the free amino and carboxylic moieties assist in water solubility. Thus, the clusters are also environmentally friendly as the capped peptide is made up of only naturally occurring protein amino acids...
March 16, 2017: Nanoscale
https://www.readbyqxmd.com/read/28297774/-effects-of-wild-type-pten-overexpression-and-its-mutation-on-f-actin-in-activated-hepatic-stellate-cells
#5
L S Hao, Y L Liu, G L Zhang, J Chen, X J Song, Y L Wang, J Wang, L M Jin
Objective: To investigate the effect of overexpression of wild-type phosphatase and tensin homolog (PTEN) deleted on chromosome 10 and its mutant G129E (exhibiting the activity of protein phosphatase and losing the activity of lipid phosphatase) on F-actin in activated hepatic stellate cells (HSCs) cultured in vitro. Methods: The activated hepatic stellate cell-T6 (HSC-T6) cells were cultured in vitro, and activated HSCs were transfected with adenovirus that carried wild-type PTEN gene and G129E gene using transient transfection...
January 20, 2017: Zhonghua Gan Zang Bing za Zhi, Zhonghua Ganzangbing Zazhi, Chinese Journal of Hepatology
https://www.readbyqxmd.com/read/28296188/white-emissive-self-assembled-organic-microcrystals
#6
Zhi Zhou Li, Feng Liang, Ming Peng Zhuo, Ying Li Shi, Xue Dong Wang, Liang Sheng Liao
Organic semiconductor micro-/nanocrystals with regular shapes have been demonstrated for many applications, such as organic field-effect transistors, organic waveguide devices, organic solid-state lasers, and therefore are inherently ideal building blocks for the key circuits in the next generation of miniaturized optoelectronics. In the study, blue-emissive organic molecules of 1,4-bis(2-methylstyryl)benzene (o-MSB) can assemble into rectangular microcrystals at a large scale via the room-temperature solution-exchange method...
March 10, 2017: Small
https://www.readbyqxmd.com/read/28296131/the-reproductive-strategy-and-the-vibrational-duet-of-the-leafhopper-empoasca-vitis-g%C3%A3-the
#7
Rachele Nieri, Valerio Mazzoni
The recent description of a new vibrational mating disruption method to control the leafhopper Scaphoideus titanus Ball opened questions about its possible application to other leafhopper pests. Since the prerequisite for the method's successful application is a deep knowledge of the species mating behavior and the exact role of associated signals, we conducted behavioral assays on the green leafhopper Empoasca vitis Göthe, a pest of grapevine and other crops in Europe and Asia. Laser vibrometer recordings of single and paired individuals (male and female) during a 24-hour period enabled us to detect and describe two male and one female signal...
March 15, 2017: Insect Science
https://www.readbyqxmd.com/read/28295085/carrier-envelope-offset-frequency-detection-and-stabilization-of-a-diode-pumped-mode-locked-ti-sapphire-laser
#8
Kutan Gürel, Valentin J Wittwer, Sargis Hakobyan, Stéphane Schilt, Thomas Südmeyer
We demonstrate the first diode-pumped Ti:sapphire laser frequency comb. It is pumped by two green laser diodes with a total pump power of 3 W. The Ti:sapphire laser generates 250 mW of average output power in 61-fs pulses at a repetition rate of 216 MHz. We generated an octave-spanning supercontinuum spectrum in a photonic-crystal fiber and detected the carrier envelope offset (CEO) frequency in a standard f-to-2f interferometer setup. We stabilized the CEO-frequency through direct current modulation of one of the green pump diodes with a feedback bandwidth of 55 kHz limited by the pump diode driver used in this experiment...
March 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/28292878/pneumonia-treatment-by-photodynamic-therapy-with-extracorporeal-illumination-an-experimental-model
#9
Mariana C Geralde, Ilaiáli S Leite, Natalia M Inada, Ana Carolina G Salina, Alexandra I Medeiros, Wolfgang M Kuebler, Cristina Kurachi, Vanderlei S Bagnato
Infectious pneumonia is a major cause of morbidity/mortality, mainly because of the increasing rate of microorganisms resistant to antibiotics. Photodynamic Therapy (PDT) is emerging as a promising approach, as effects are based on oxidative stress, preventing microorganism resistance. In two previous studies, the in vitro inactivation of Streptococcus pneumoniae using indocyanine green (ICG) and infrared light source was a success killing 5 log10 colony-forming units (CFU/mL) with only 10 μmol/L ICG. In this work, a proof-of-principle protocol was designed to treat lung infections by PDT using extracorporeal illumination with a 780 nm laser device and also ICG as photosensitizer...
March 2017: Physiological Reports
https://www.readbyqxmd.com/read/28288450/laser-treatment-of-tattoos-basic-principles
#10
Wolfgang Bäumler
Tattooing has become very popular worldwide during the past decades, and millions of people have one or many tattoos at different anatomical sites. The color of tattoos is mainly black, followed by red, green, blue, and other colors. A part of the tattooed people regret tattooing or have permanent problems with tattoos and therefore seek for tattoo removal. Tattoos consist of solid pigment particles in the skin. Thus, tattoo removal requires fragmentation of these permanently incorporated particles. The gold standard of tattoo removal is laser therapy...
2017: Current Problems in Dermatology
https://www.readbyqxmd.com/read/28280483/outer-plexiform-layer-structures-are-not-altered-following-aav-mediated-gene-transfer-in-healthy-rat-retina
#11
Bert C Giers, Daniela Klein, Alexandra Mendes-Madeira, Carolina Isiegas, Birgit Lorenz, Silke Haverkamp, Knut Stieger
Ocular gene therapy approaches have been developed for a variety of different diseases. In particular, clinical gene therapy trials for RPE65 mutations, X-linked retinoschisis, and choroideremia have been conducted at different centers in recent years, showing that adeno-associated virus (AAV)-mediated gene therapy is safe, but limitations exist as to the therapeutic benefit and long-term duration of the treatment. The technique of vector delivery to retinal cells relies on subretinal injection of the vector solution, causing a transient retinal detachment...
2017: Frontiers in Neurology
https://www.readbyqxmd.com/read/28277843/nanoethosomal-formulation-of-gammaoryzanol-for-skin-aging-protection-and-wrinkle-improvement-a-histopathological-study
#12
Saman Heydari, Saeed Ghanbarzadeh, Behzad Anoosh, Mohammad Reza Ranjkesh, Yousef Javadzadeh, Maryam Kouhsoltani, Hamed Hamishehkar
BACKGROUND: Free radical scavengers and antioxidants, with the main focus on enhanced targeting to the skin layers, can provide protection against skin ageing. OBJECTIVE: The aim of the present study was to prepare nanoethosomal formulation of Gammaoryzanol (GO), a water insoluble antioxidant, for its dermal delivery to prevent skin aging. METHODS: Nanoethosomal formulation was prepared by a modified ethanol injection method and characterized by using laser light scattering, scanning electronic microscope (SEM) and X-ray diffraction (XRD) techniques...
March 3, 2017: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/28277002/folate-modified-indocyanine-green-loaded-lipid-polymer-hybrid-nanoparticles-for-targeted-delivery-of-cisplatin
#13
Lianshuai Gu, Tianyi Shi, Yu Sun, Chaoqun You, Senlin Wang, Gaoju Wen, Lan Chen, Xiangyang Zhang, Jin Zhu, Baiwang Sun
Cisplatin is a potent antitumor drug, which is widely applied in clinical cancer treatment. However, cisplatin can hardly distinguish between healthy tissue and tumor tissue, resulting in serious toxic side effects. Indocyanine green (ICG) is a FDA-approved near-infrared (NIR) fluorescence dye which has been used in photothermal therapy and optically mediated diagnostic, but the application of ICG is limited by its concentration-dependent aggregation, poor aqueous stability in vitro, lack of target specificity and rapid elimination from the body...
May 2017: Journal of Biomaterials Science. Polymer Edition
https://www.readbyqxmd.com/read/28269425/green-lasers-are-beyond-power-limits-mandated-by-safety-standards
#14
M H Lee, K Fox, S Goldwasser, D W M Lau, B Aliahmad, M Sarossy
There has been an increasing number of reports of people losing vision from laser exposure from pocket laser pointers despite the safety limit of 1 milliwatt (1mW) imposed by the Australian government. We hypothesize that this is because commercially available red and green laser pointers are exceeding their labeled power outputs. We tested the power outputs of 4 red and 4 green lasers which were purchased for less than AUD$30 each. The average of 10 measurements was recorded for each laser. We found that 3 out of 4 red lasers conformed to the 1mW safety standard; in contrast, all of the green lasers exceeded this limit, with one of the lasers recording an output of 127...
August 2016: Conference Proceedings: Annual International Conference of the IEEE Engineering in Medicine and Biology Society
https://www.readbyqxmd.com/read/28253064/effects-of-two-diode-lasers-with-and-without-photosensitization-on-contaminated-implant-surfaces-an-ex-vivo-study
#15
Nicola Alberto Valente, Thomas Mang, Michael Hatton, Lynn Mikulski, Sebastiano Andreana
OBJECTIVE: The aim of this ex vivo study is to assess decontamination potential of two different diode laser wavelengths, with or without the aid of photodynamic therapy, on dental implant surfaces and to evaluate the harmful potential of temperature increase during laser irradiation. MATERIALS AND METHODS: One hundred thirty-two machined sterile implants were placed into sterile porcine bone blocks with standardized coronal angular bony defects and inoculated with Streptococcus sanguinis...
February 16, 2017: Photomedicine and Laser Surgery
https://www.readbyqxmd.com/read/28249365/coherent-light-photo-modification-mass-transport-effect-and-surface-relief-formation-in-asxs100-x-nanolayers-absorption-edge-xps-and-raman-spectroscopy-combined-with-profilometry-study
#16
O Kondrat, R Holomb, A Csik, V Takáts, M Veres, V Mitsa
As x S100-x (x = 40, 45, 50) thin films top surface nanolayers affected by green (532 nm) diode laser illumination have been studied by high-resolution X-ray photoelectron spectroscopy, Raman spectroscopy, optical spectroscopy, and surface profilometry. It is shown that the composition of obtained films depends not only on the composition of the source material but as well on the composition of the vapor during the evaporation process. Near-bandgap laser light decreases both As-As and S-S homopolar bonds in films, obtained from thermal evaporation of the As40S60 and As50S50 glasses...
December 2017: Nanoscale Research Letters
https://www.readbyqxmd.com/read/28248826/retinal-vascular-abnormalities-in-a-large-cohort-of-patients-affected-by-neurofibromatosis-type-1-a-study-using-optical-coherence-tomography-angiography
#17
Raffaele Parrozzani, Elisabetta Pilotto, Maurizio Clementi, Luisa Frizziero, Francesca Leonardi, Enrica Convento, Giacomo Miglionico, Serena Pulze, Pierdavide Perrini, Eva Trevisson, Matteo Cassina, Edoardo Midena
PURPOSE: To evaluate the prevalence, the vascular features, and the clinical diagnostic implication of retinal vascular abnormalities (RVAs) associated with neurofibromatosis Type 1 (NF1) in a large cohort of patients. METHODS: Two hundred and ninety-four patients affected by NF1 were consecutively enrolled. The presence of RVAs was detected by means of infrared confocal scanning laser ophthalmoscopy images. Three hundred age- and race-matched healthy subjects were enrolled as a healthy control group...
February 28, 2017: Retina
https://www.readbyqxmd.com/read/28243562/microfluidic-setup-for-on-line-sers-monitoring-using-laser-induced-nanoparticle-spots-as-sers-active-substrate
#18
Oana-M Buja, Ovidiu D Gordan, Nicolae Leopold, Andreas Morschhauser, Jörg Nestler, Dietrich R T Zahn
A microfluidic setup which enables on-line monitoring of residues of malachite green (MG) using surface-enhanced Raman scattering (SERS) is reported. The SERS active substrate was prepared via laser induced synthesis of silver or gold nanoparticles spot on the bottom of a 200 μm inner dimension glass capillary, by focusing the laser beam during a continuous flow of a mixture of silver nitrate or gold chloride and sodium citrate. The described microfluidic setup enables within a few minutes the monitoring of several processes: the synthesis of the SERS active spot, MG adsorption to the metal surface, detection of the analyte when saturation of the SERS signal is reached, and finally, the desorption of MG from the spot...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28243557/laser-irradiation-in-water-for-the-novel-scalable-synthesis-of-black-tio-x-photocatalyst-for-environmental-remediation
#19
Massimo Zimbone, Giuseppe Cacciato, Mohamed Boutinguiza, Vittorio Privitera, Maria Grazia Grimaldi
Since 1970, TiO2 photocatalysis has been considered a possible alternative for sustainable water treatment. This is due to its material stability, abundance, nontoxicity and high activity. Unfortunately, its wide band gap (≈3.2 eV) in the UV portion of the spectrum makes it inefficient under solar illumination. Recently, so-called "black TiO2" has been proposed as a candidate to overcome this issue. However, typical synthesis routes require high hydrogen pressure and long annealing treatments. In this work, we present an industrially scalable synthesis of TiO2-based material based on laser irradiation...
2017: Beilstein Journal of Nanotechnology
https://www.readbyqxmd.com/read/28240270/influence-of-doping-and-excitation-powers-on-optical-thermometry-in-yb-3-er-3-doped-cawo4
#20
Xiangfu Wang, Yemin Wang, Yanyan Bu, Xiaohong Yan, Jing Wang, Peiqing Cai, Thiquynh Vu, Hyo Jin Seo
Optical thermometry has been widely studied to achieve an inaccessible temperature measurement in submicron scale and it has been reported that the temperature sensitivity depends mainly on host types. In this work, we propose a new method to improve the optical temperature sensitivity of Yb(3+)-Er(3+) co-doped CaWO4 phosphors by doping with Li(+), Sr(2+), and Mg(2+) ions and by controlling excitation powers of 980 nm laser. It is found that the thermometric parameters such as upconversion emission intensity, intensity ratio of green-to-red emission, fluorescence color, emission intensity ratios of thermally coupled levels ((2)H11/2/(4)S3/2), and relative and absolute temperature sensitivity can be effectively controlled by doping with Li(+), Sr(2+), and Mg(2+) ions in the Yb(3+)-Er(3+) co-doped CaWO4 system...
February 27, 2017: Scientific Reports
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