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https://www.readbyqxmd.com/read/29786124/evaluation-of-the-relationship-between-flap-tension-and-tissue-perfusion-in-implant-based-breast-reconstruction-using-laser-assisted-indocyanine-green-angiography
#1
Chae-Eun Yang, Soon Won Chung, Dong Won Lee, Dae Hyun Lew, Seung Yong Song
BACKGROUND: For implant-based breast reconstruction, inadequate tissue perfusion may cause devastating complications. Tissue tension by inadequate implant volume may reduce tissue perfusion by stretching and collapsing the capillaries. The SPY system is used to perform intraoperative fluorescence angiography with indocyanine green to assess visually the blood flow and evaluate tissue perfusion. However, there is no report yet about how mastectomy flap perfusion changes with the expander-filling volume...
May 21, 2018: Annals of Surgical Oncology
https://www.readbyqxmd.com/read/29783745/loading-of-indocyanine-green-within-polydopamine-coated-laponite-nanodisks-for-targeted-cancer-photothermal-and-photodynamic-therapy
#2
Fanli Xu, Mengxue Liu, Xin Li, Zhijuan Xiong, Xueyan Cao, Xiangyang Shi, Rui Guo
The combination of photothermal therapy (PTT) and photodynamic therapy (PDT) in cancer treatment has attracted much attention in recent years. However, developing highly efficient and targeted therapeutic nanoagents for amplifying PTT and PDT treatments remains challenging. In this work, we developed a novel photothermal and photodynamic therapeutic nanoplatform for treatment of cancer cells overexpressing integrin αv β₃ through the coating of polydopamine (PDA) on indocyanine green (ICG)-loaded laponite (LAP) and then further conjugating polyethylene glycol-arginine-glycine-aspartic acid (PEG-RGD) as targeted agents on the surface...
May 19, 2018: Nanomaterials
https://www.readbyqxmd.com/read/29782671/enhanced-performance-of-a-molecular-photoacoustic-imaging-agent-by-encapsulation-in-mesoporous-silicon-nanoparticles
#3
Jinyoung Kang, Dokyoung Kim, Junxin Wang, Yunho Han, Jonathan M Zuidema, Ali Hariri, Ji-Ho Park, Jesse V Jokerst, Michael J Sailor
Photoacoustic (PA) imaging allows visualization of the physiology and pathology of tissues with good spatial resolution and relatively deep tissue penetration. The method converts near-infrared (NIR) laser excitation into thermal expansion, generating pressure transients that are detected with an acoustic transducer. Here, we find that the response of the PA contrast agent indocyanine green (ICG) can be enhanced 17-fold when it is sealed within a rigid nanoparticle. ICG encapsulated in particles composed of porous silicon (pSiNP), porous silica, or calcium silicate all show greater PA contrast relative to equivalent quantities of free ICG, with the pSiNPs showing the strongest enhancement...
May 21, 2018: Advanced Materials
https://www.readbyqxmd.com/read/29782140/fluorescent-labeling-of-the-nuclear-envelope-by-localizing-gfp-on-the-inner-nuclear-membrane
#4
Toshiyuki Taniyama, Natsumi Tsuda, Shinji Sueda
The nuclear envelope (NE) is a double membrane that segregates nuclear components from the cytoplasm in eukaryote cells. It is well-known that the NE undergoes a breakdown and reformation during mitosis in animal cells. However, the detailed mechanisms of the NE dynamics are not yet fully understood. Here, we propose a method for the fluorescent labeling of the NE in living cells, which enables the tracing of the NE dynamics during cell division under physiological conditions. In our method, labeling of the NE is accomplished by fixing green fluorescent protein carrying the nuclear localization signal on the inner nuclear membrane based on a unique biotinylation reaction from the archaeon Sulfolobus tokodaii...
May 21, 2018: ACS Chemical Biology
https://www.readbyqxmd.com/read/29781903/treatment-of-corticosteroid-induced-hypopigmentation-using-fractional-carbon-dioxide-laser
#5
Margaret C Green, Michael S Tracey, John P Trafeli, John Paul Trafeli
No abstract text is available yet for this article.
May 16, 2018: Dermatologic Surgery: Official Publication for American Society for Dermatologic Surgery [et Al.]
https://www.readbyqxmd.com/read/29781161/a-novel-titanium-sapphire-picosecond-domain-laser-safely-and-effectively-removes-purple-blue-and-green-tattoo-inks
#6
Eric F Bernstein, Jayant Bhawalkar, Kevin T Schomacker
BACKGROUND: Green, blue, and purple tattoo pigments are often the colors most resistant to laser removal. Recently, the first ever production picosecond-domain laser with a 785 nm wavelength was developed to improve the rate of clearance of green, blue, and purple tattoo inks. METHODS: Twenty-two tattoos from 15 subjects with skin phototypes II-IV were enrolled in the study. A total of four treatments were administered using a single 785 nm picosecond-domain laser wavelength...
May 20, 2018: Lasers in Surgery and Medicine
https://www.readbyqxmd.com/read/29777957/dual-ph-reduction-responsive-hybrid-polymeric-micelles-for-targeted-chemo-photothermal-combination-therapy
#7
Linhua Zhang, Yu Qin, Zhiming Zhang, Fan Fan, Chenlu Huang, Li Lu, Hai Wang, Xu Jin, Hanxue Zhao, Deling Kong, Chun Wang, Hongfan Sun, Xigang Leng, Dunwan Zhu
The combination of chemotherapy and photothermal therapy in multifunctional nanovesicles has emerged as a promising strategy to improve cancer therapeutic efficacy. Herein, we designed new pH/reduction dual-responsive and folate decorated polymeric micelles (FA Co-PMs) as theranostic nanocarrier to co-encapsulate doxorubicin (DOX) and indocyanine green (ICG) for targeted NIR imaging and chemo-photothermal combination therapy. The Co-PMs exhibited nano-sized structure (∼100 nm) with good monodispersity, high encapsulation efficiency of both ICG and DOX, triggered DOX release in response to acid pH and reduction environment, and excellent temperature conversion with laser irradiation...
May 16, 2018: Acta Biomaterialia
https://www.readbyqxmd.com/read/29769391/infrared-laser-mediated-gene-induction-at-the-single-cell-level-in-the-regenerating-tail-of-xenopus-laevis-tadpoles
#8
Riho Hasugata, Shinichi Hayashi, Aiko Kawasumi-Kita, Joe Sakamoto, Yasuhiro Kamei, Hitoshi Yokoyama
We describe a precise and reproducible gene-induction method in the amphibian, Xenopus laevis Tetrapod amphibians are excellent models for studying the mechanisms of three-dimensional organ regeneration because they have an exceptionally high regenerative ability. However, spatial and temporal manipulation of gene expression has been difficult in amphibians, hindering studies on the molecular mechanisms of organ regeneration. Recently, however, development of a Xenopus transgenic system with a heat-shock-inducible gene has enabled the manipulation of specific genes...
May 16, 2018: Cold Spring Harbor Protocols
https://www.readbyqxmd.com/read/29767415/micellized-tris-bipyridine-ruthenium-catalysts-affording-preparative-amounts-of-hydrated-electrons-with-a-green-light-emitting-diode
#9
Robert Naumann, Florian Lehmann, Martin Goez
We have explored alkyl substitution of the ligands as a means to improve the performance of the title complexes in photoredox catalytic systems that produce synthetically useable amounts of hydrated electrons through photon pooling. Despite generating a superreductant, these electron sources only consume the bioavailable ascorbate and are driven by a green light-emitting diode (LED). The substitutions influence the catalyst activity through the quenching and recombination rates across the micelle-water interface, and the photoionization quantum yield...
May 16, 2018: Chemistry: a European Journal
https://www.readbyqxmd.com/read/29764213/a-novel-non-invasive-strategy-for-low-level-laser-induced-cancer-therapy-by-using-new-ag-zno-and-nd-zno-functionalized-reduced-graphene-oxide-nanocomposites
#10
Saeed Jafarirad, Ebrahim Hammami Torghabe, Seyed Hosseyn Rasta, Roya Salehi
In the present research, an effective drug-free approach was developed to kill MCF7 breast cancer cells using low-level laser therapy (LLLT) combined with reduced graphene oxide (rGO)-based hybrid nanocomposites (NCs). Here, fruit extract of Rosa canina was used for the first time to obtain the rGO/ZnO, Ag-ZnO/rGO and Nd-ZnO/rGO NCs by green synthesis. Physico/photochemical properties of these NCs were evaluated using FTIR, XRD, Raman, XPS, SEM/EDX, UV-Vis, DLS and AFM. The potential of the as-synthesized NCs on ROS generating and antioxidant activity were assessed by DPPH...
May 15, 2018: Artificial Cells, Nanomedicine, and Biotechnology
https://www.readbyqxmd.com/read/29763825/reconstructing-the-colour-palette-of-the-konstantinos-parthenis-burnt-paintings
#11
N Antonopoulou-Athera, E Chatzitheodoridis, A Terlixi, M Doulgerides, A A Serafetinides
This case study focuses on the reconstruction of the colour palette and the possibility of laser cleaning of burnt paintings. The paintings ORPHEUS IN THE UNDERWORLD and CONCORDIA, composed by the Greek artist Konstantinos Parthenis (1878-1967), have been severely damaged by fire. The colour palette of Parthenis is thoroughly investigated for the first time, and to perform this, a multi-analytical spectroscopic approach was employed. Non-destructive in situ analysis was performed on multiple areas of the paintings by portable XRF...
May 9, 2018: Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy
https://www.readbyqxmd.com/read/29762623/nanowire-lasers-as-intracellular-probes
#12
Xiaoqin Wu, Qiushu Chen, Peizhen Xu, Yu-Cheng Chen, Biming Wu, Rhima M Coleman, Limin Tong, Xudong Fan
We investigate a cadmium sulfide (CdS) nanowire (NW) laser that is spontaneously internalized into a single cell to serve as a stand-alone intracellular probe. By pumping with nano-joule light pulses, green laser emission (500-520 nm) can be observed inside cells with a peak linewidth as narrow as 0.5 nm. Due to the sub-micron diameter (∼200 nm), the NW has an appreciable fraction of the evanescent field outside, facilitating a sensitive detection of cellular environmental changes. By monitoring the lasing peak wavelength shift in response to the intracellular refractive index change, our NW laser probe shows a sensitivity of 55 nm per RIU (refractive index units) and a figure of merit of approximately 98...
May 15, 2018: Nanoscale
https://www.readbyqxmd.com/read/29757281/analyses-of-mitochondrial-calcium-influx-in-isolated-mitochondria-and-cultured-cells
#13
Joshua T Maxwell, Chin-Hsien Tsai, Tahmina A Mohiuddin, Jennifer Q Kwong
Ca2+ handling by mitochondria is a critical function regulating both physiological and pathophysiological processes in a broad spectrum of cells. The ability to accurately measure the influx and efflux of Ca2+ from mitochondria is important for determining the role of mitochondrial Ca2+ handling in these processes. In this report, we present two methods for the measurement of mitochondrial Ca2+ handling in both isolated mitochondria and cultured cells. We first detail a plate reader-based platform for measuring mitochondrial Ca2+ uptake using the Ca2+ sensitive dye calcium green-5N...
April 27, 2018: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29756764/formation-mechanism-structural-and-upconversion-properties-of-alkaline-rare-earth-fluoride-nanocrystals-doped-with-yb-3-er-3-ions
#14
Tomasz Grzyb, Dominika Przybylska
Ultrasmall (9-30 nm) Yb3+ /Er3+ -doped, upconverting alkaline rare-earth fluorides that are promising for future applications were synthesized by the microwave-assisted hydrothermal method. The formation mechanism was proposed, indicating the influence of the stability of metal ions complexes with ethylenediaminetetraacetic acid on the composition of the product and tendency to form M2 REF7 (M0.67 RE0.33 F2.33 ) cubic compounds in the M-RE-F systems. Their physicochemical properties (structure, morphology, and spectroscopic properties) are compared and discussed...
May 14, 2018: Inorganic Chemistry
https://www.readbyqxmd.com/read/29752644/a-new-candidate-laser-dye-based-1-4-bis-%C3%AE-2-naphthothiazolyle-vinyl-benzene-spectroscopic-behavior-laser-parameters-and-excitation-energy-transfer
#15
Abeer N Al-Romaizan, Mahmoud A Hussein
A new candidate laser dye based 1,4-bis[β-(2-naphthothisolyl) vinyl] benzene (BNTVB) were prepared, and characterized in various organic solvents. The center polarity is less sensitive than electronic absorption. A red shift was noticed in the fluorescence spectra (ca. 40 nm) with increment in the solvent's polarity, this means that BNTVB's polarity appreciates upon excitation. The dipole moment of ground state (μg ) and the excited singlet state dipole moment (μe ) are determined from Kawski - Chamma and Bakshiev-Viallet equations using the disparity of Stokes shift with solvent polarity function of ε (dielectric constant) and n (refractive index) of the solvent...
May 12, 2018: Journal of Fluorescence
https://www.readbyqxmd.com/read/29749937/risk-of-collateral-lead-damage-in-percutaneous-cardiac-implantable-electronic-device-extraction
#16
Faris Khan, Gustaf Sverin, Ulrika Birgersdotter-Green, Jennifer P Miller, Gautam Lalani, Travis Pollema, Victor Pretorius
OBJECTIVES: This study sought to assess the risk of collateral lead damage during cardiac implantable electronic device extraction. BACKGROUND: With the increasing numbers of cardiovascular implantable electronic devices, there has been an increase in the number of percutaneous device and lead extractions. It is unknown how often collateral damage (defined as the need for unintended lead extraction, or loss of lead's integrity or dislodgement) occurs in the planned retained leads...
February 2018: JACC. Clinical Electrophysiology
https://www.readbyqxmd.com/read/29749727/in-situ-crystallization-synthesis-of-cspbbr3-perovskite-quantum-dots-embedded-glasses-with-improved-stability-for-solid-state-lighting-and-random-upconverted-lasing
#17
Shuo Yuan, Daqin Chen, Xinyue Li, Jiasong Zhong, Xuhui Xu
All-inorganic cesium lead bromide CsPbBr3 perovskite quantum dots (QDs) are emerging as potential candidates for the applications in optoelectronic devices but suffer from poor long-term stability due to their high sensitivity to UV irradiation, heat and especially to moisture. Although great advances in improving stability of perovskite QDs have been achieved by surface modification or encapsulation in polymer and silica, they are not sufficiently refrained from external environment due to non-dense structures of these protective layers...
May 11, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29748644/live-cell-imaging-of-nuclear-chromosomal-dynamics-in-bovine-in-vitro-fertilised-embryos
#18
Tatsuma Yao, Rie Suzuki, Natsuki Furuta, Yuka Suzuki, Kyoko Kabe, Mikiko Tokoro, Atsushi Sugawara, Akira Yajima, Tomohiro Nagasawa, Satoko Matoba, Kazuo Yamagata, Satoshi Sugimura
Nuclear/chromosomal integrity is an important prerequisite for the assessment of embryo quality in artificial reproductive technology. However, lipid-rich dark cytoplasm in bovine embryos prevents its observation by visible light microscopy. We performed live-cell imaging using confocal laser microscopy that allowed long-term imaging of nuclear/chromosomal dynamics in bovine in vitro fertilised (IVF) embryos. We analysed the relationship between nuclear/chromosomal aberrations and in vitro embryonic development and morphological blastocyst quality...
May 10, 2018: Scientific Reports
https://www.readbyqxmd.com/read/29744752/indocyanine-green-mediated-photobiomodulation-on-human-osteoblast-cells
#19
Gamze Bölükbaşı Ateş, Ayşe Ak, Bora Garipcan, Murat Gülsoy
Photobiomodulation (PBM) and photodynamic therapy (PDT) share similar mechanisms but have opposite aims. Increased levels of reactive oxygen species (ROS) in the target tissue in response to light combined photosensitizer (PS) application may lead to cell proliferation or oxidative damage depending on the ROS amount. The purpose of the present study is to investigate the effect of indocyanine green (ICG)-mediated PBM on osteoblast cells by measuring cell viability, proliferation, alkaline phosphatase (ALP) activity, mineralization, and gene expressions of three phenotypic osteoblast markers...
May 9, 2018: Lasers in Medical Science
https://www.readbyqxmd.com/read/29739918/electrospinning-of-highly-concentrated-albumin-patches-by-using-auxiliary-polymers-for-laser-assisted-vascular-anastomosis
#20
Annemarie Schönfeld, Mihai Constantinescu, Kirsten Peters, Martin Frenz
Electrospun meshes have been extensively investigated for tissue engineering and drug delivery. The application of this technology is of interest for laser-assisted vascular anastomosis (LAVA) due to the possibility to bind and stabilize macromolecules in fibers.
 Materials and Methods: We prepared bovine serum albumin (BSA) blend microfibers from the auxiliary proteins polyethylene oxide (PEO), polycaprolactone (PCL), polyvinyl alcohol (PVA) and gelatin. The thickness and weight of the resulting patches were measured and the morphological characteristics were observed by scanning electron microscopy...
May 9, 2018: Biomedical Materials
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