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https://www.readbyqxmd.com/read/29156586/in-depth-glyco-peptidomics-approach-reveals-unexpected-diversity-of-glycosylated-peptides-and-atypical-post-translational-modifications-in-dendroaspis-angusticeps-snake-venom
#1
Michel Degueldre, Julien Echterbille, Nicolas Smargiasso, Christian Damblon, Charlotte Gouin, Gilles Mourier, Nicolas Gilles, Edwin De Pauw, Loïc Quinton
Animal venoms represent a valuable source of bioactive peptides that can be derived into useful pharmacological tools, or even innovative drugs. In this way, the venom of Dendroaspis angusticeps (DA), the Eastern Green Mamba, has been intensively studied during recent years. It mainly contains hundreds of large toxins from 6 to 9 kDa, each displaying several disulfide bridges. These toxins are the main target of venom-based studies due to their valuable activities obtained by selectively targeting membrane receptors, such as ion channels or G-protein coupled receptors...
November 18, 2017: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29155795/in-vivo-imaging-of-cx3cr1gfp-gfp-reporter-mice-with-spectral-domain-optical-coherence-tomography-and-scanning-laser-ophthalmoscopy
#2
Despina Kokona, Joël Jovanovic, Andreas Ebneter, Martin S Zinkernagel
Spectral domain optical coherence tomography (SD-OCT) and scanning laser ophthalmoscopy (SLO) are extensively used in experimental ophthalmology. In the present protocol, mice expressing green fluorescent protein (gfp) under the promoter of Cx3cr1 (BALB/c-Cx3cr1(gfp/gfp)) were used to image microglia cells in vivo in the retina. Microglia are resident macrophages of the retina and have been implicated in several retinal diseases(1)(,)(2)(,)(3)(,)(4)(,)(5)(,)(6). This protocol provides a detailed approach for generation of retinal B-scans, with SD-OCT, and imaging of microglia cell distribution in Cx3cr1(gfp/gfp) mice with SLO in vivo, using an ophthalmic imaging platform system...
November 11, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/29155399/green-synthesis-of-anisotropic-gold-nanoparticles-using-hordenine-and-their-antibiofilm-efficacy-against-pseudomonas-aeruginosa
#3
Jobina Rajkumari, Himani Meena, Muralitharan Gangatharan, Siddhardha Busi
Pseudomonas aeruginosa is a notorious pathogen that causes biofilm aided infections in patients with cystic fibrosis and burn wounds, resulting in significant mortality in immunocompromised individuals. This study reports a novel one-step biosynthesis of gold nanoparticles using phytocompound, hordenine (HD), as a reducing and capping agent. The synthesis of the anisotropic hordenine-fabricated gold nanoparticles (HD-AuNPs) with an average particle size of 136.87 nm was achieved within 12 h of incubation at room temperature...
December 2017: IET Nanobiotechnology
https://www.readbyqxmd.com/read/29148176/differential-proteomic-analysis-reveals-protein-networks-and-pathways-that-may-contribute-to-helicobacter-pylori-fkbp-type-ppiase-associated-gastric-diseases
#4
Yanmei Zhu, Yuehua Gong, Aodi Li, Moye Chen, Dan Kang, Jun Liu, Yuan Yuan
PURPOSE: Though Helicobacter pylori (H. pylori) has been classified as class I carcinogen, key virulence factor generated by H. pylori that causes gastric cancer remains to be fully determined. Recently, we identified a gastric cancer-associated H. pylori gene, peptidylprolyl isomerase-FK506 binding protein (PPIase-FKBP), and showed that PPIase-FKBP was capable of inducing oncogenic transformation of gastric epithelial cells. But its mechanism was unclear. EXPERIMENTAL DESIGN: We carried out a comparative proteomic analysis of human gastric epithelial cells that either express PPIase-FKBP or green fluorescent protein (GFP) using two-dimensional (2-D) gel electrophoresis and then matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF-MS/MS)...
November 16, 2017: Proteomics. Clinical Applications
https://www.readbyqxmd.com/read/29147000/stable-icg-loaded-upconversion-nanoparticles-silica-core-shell-theranostic-nanoplatform-for-dual-modal-upconversion-and-photoacoustic-imaging-together-with-photothermal-therapy
#5
Ruichan Lv, Depeng Wang, Liyang Xiao, Guanying Chen, Jun Xia, Paras N Prasad
We report here the design and multiple functions of a new hierarchical nanotheronostic platform consisting of an upconversion nanoparticle (UCNP) core: shell with an additional mesoporous silica (mSiO2) matrix load shell containing sealed, high concentration of ICG molecules. We demonstrate that this UCNP@mSiO2-ICG nanoplatform can perform the following multiple functions under NIR excitation at 800 nm: 1) Light harvesting by the UCNP shell containing Nd and subsequent energy transfer to Er in the Core to produce efficient green and red upconversion luminescence for optical imaging; 2) Efficient nonradiative relaxation and local heating produced by concentration quenching in aggregated ICG imbedded in the mesopourous silica shell to enable both photoacoustic imaging and photothermal therapy...
November 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/29144425/selective-laser-sintering-of-porous-silica-enabled-by-carbon-additive
#6
Shuai Chang, Liqun Li, Li Lu, Jerry Ying Hsi Fuh
The aim of this study is to investigate the possibility of a freeform fabrication of porous ceramic parts through selective laser sintering (SLS). SLS was proposed to manufacture ceramic green parts because this additive manufacturing technique can be used to fabricate three-dimensional objects directly without a mold, and the technique has the capability of generating porous ceramics with controlled porosity. However, ceramic printing has not yet fully achieved its 3D fabrication capabilities without using polymer binder...
November 16, 2017: Materials
https://www.readbyqxmd.com/read/29144120/plasmon-induced-selective-enhancement-of-green-emission-in-lanthanide-doped-nanoparticles
#7
Weina Zhang, Juan Li, Hongxiang Lei, Baojun Li
By introducing an 18 nm-thick Au nanofilm, selective enhancement of green emission from Lanthanide-doped (β-NaYF4: Yb3+/Er3+) upconversion nanoparticles (UCNPs) is demonstrated. The Au nanofilm is deposited on a microfiber surface by the sputtering method and then covered with the UCNPs. The plasma on the surface of the Au nanofilm can be excited by launching a 980 nm-wavelength laser beam into the microfiber, resulting in an enhancement of the local electric field and a strong thermal effect. A 36-fold luminescence intensity enhancement of the UCNPs at 523 nm is observed with no obvious reduction in the photostability of the UCNPs...
November 16, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29140861/sealing-of-corneal-lacerations-using-photoactivated-rose-bengal-dye-and-amniotic-membrane
#8
Timothy A Soeken, Hong Zhu, Sheri DeMartelaere, Brett W Davies, Mirang Kim, Heuy-Ching Wang, James Aden, Rose Grimm, Clemens Alt, Irene E Kochevar, Anthony J Johnson
PURPOSE: Watertight closure of perforating corneoscleral lacerations is necessary to prevent epithelial ingrowth, infection, and potential loss of the eye. Complex lacerations can be difficult to treat, and repair with sutures alone is often inadequate. In this study, we evaluated a potentially sutureless technology for sealing complex corneal and scleral lacerations that bonds the amniotic membrane (AM) to the wound using only green light and rose bengal dye. METHODS: The AM was impregnated with rose bengal and then sealed over lacerations using green light to bond the AM to the deepithelialized corneal surface...
November 14, 2017: Cornea
https://www.readbyqxmd.com/read/29140343/passively-q-switched-pr-ylf-laser-with-a-co-2-mgal2o4-saturable-absorber
#9
Maxim Demesh, Daniel-Timo Marzahl, Anatol Yasukevich, Viktor Kisel, Günter Huber, Nikolay Kuleshov, Christian Kränkel
We report on a 2ω-OPSL-pumped passively Q-switched Pr(3+):LiYF4 laser operating at the wavelengths of 523, 607, and 640 nm. For this, we utilized a Co(2+)-doped MgAl2O4 (MALO) crystal as a saturable absorber in the visible range for the first time, to the best of our knowledge. In the green spectral region, the pulse duration was 210 ns with a pulse energy of 3.6 μJ and a repetition rate of 125 kHz. The minimum pulse duration of 87 ns at the highest pulse energy of 8.6 μJ was obtained at a repetition rate of 110 kHz at 607 nm...
November 15, 2017: Optics Letters
https://www.readbyqxmd.com/read/29130364/fluorescent-labeling-and-2-photon-imaging-of-mouse-tooth-pulp-nociceptors
#10
A Kadala, P Sotelo-Hitschfeld, Z Ahmad, P Tripal, B Schmid, A Mueller, L Bernal, Z Winter, S Brauchi, U Lohbauer, K Messlinger, J K Lennerz, K Zimmermann
Retrograde fluorescent labeling of dental primary afferent neurons (DPANs) has been described in rats through crystalline fluorescent DiI, while in the mouse, this technique was achieved with only Fluoro-Gold, a neurotoxic fluorescent dye with membrane penetration characteristics superior to the carbocyanine dyes. We reevaluated this technique in the rat with the aim to transfer it to the mouse because comprehensive physiologic studies require access to the mouse as a model organism. Using conventional immunohistochemistry, we assessed in rats and mice the speed of axonal dye transport from the application site to the trigeminal ganglion, the numbers of stained DPANs, and the fluorescence intensity via 1) conventional crystalline DiI and 2) a novel DiI formulation with improved penetration properties and staining efficiency...
November 1, 2017: Journal of Dental Research
https://www.readbyqxmd.com/read/29130175/imaging-n-acyl-homoserine-lactone-quorum-sensing-in-vivo
#11
Louise Dahl Hultqvist, Maria Alhede, Tim Holm Jakobsen, Michael Givskov, Thomas Bjarnsholt
In order to study N-acyl homoserine lactone (AHL)-based quorum sensing in vivo, we present a protocol using an Escherichia coli strain equipped with a luxR-based monitor system, which in the presence of exogenous AHL molecules expresses a green fluorescent protein (GFP). Lungs from mice challenged intratracheally with alginate beads containing both a Pseudomonas aeruginosa strain together with the E. coli monitor strain can be investigated at different time points postinfection. Epifluorescent or confocal scanning laser microscopy (CSLM) is used to detect the GFP-expressing E...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29124920/nanocolloidosomes-with-selective-drug-release-for-active-tumor-targeted-imaging-guided-photothermal-chemo-combination-therapy
#12
Hang Hu, Chen Xiao, Honglian Wu, Yihui Li, Qing Zhou, Yuxiang Tang, Chan Yu, Xiangliang Yang, Zifu Li
Selective drug release is highly desirable for photothermal/chemo combination therapy when two or even more theranostic agents are encapsulated together within the same nanocarrier. Conventional nanocarrier can hardly achieve this goal. Herein, doxorubicin and indocyanine green (DOX/ICG) loaded nanocolloidosomes (NCs), with selective drug release, were fabricated as a novel multifunctional theranostic nanoplatform for photothermal/chemo combination therapy. Templating from galactose functionalized hydroxyethyl starch-polycaprolactone (Gal-HES-PCL) nanoparticles stabilized Pickering emulsions, the resultant DOX/ICG@Gal-HES-PCL NCs had a diameter around 140 nm and showed outstanding tumor targeting ability, preferable tumor penetration capability, and promoted photothermal effect...
November 10, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29123633/efficiency-of-a-new-combined-laser-therapy-in-patients-with-trophic-ulcers-of-lower-extremities-and-chronic-venous-insufficiency
#13
Sergey Vladimirovich Moskvin, Aleksandr Vladimirovich Geynitz, Evgeniy Valer'yevich Askhadulin
Introduction: Treating patients with trophic ulcers (TUs) of venous etiology (VE) is a complicated socio-medical and economic problem. One of the ways to increase treatment efficiency can be a complex therapy, including combined laser action. Methods: The objective of this study was a comparative evaluation of the efficiency of traditional treatment methods in patients with chronic venous diseases of C6 class, and combined laser therapy (LT) according to a new method by LASMIK® device. External exposure was conducted on the 1-4 affected area during one session for 2 minutes per zone (pulsed mode, light pulse duration of 100-130 ns, wavelength 635 nm, by a matrix emitter consisting of eight laser diodes with a surface area of 8 cm2, at a distance of up to 7 cm, pulsed power 40 W)...
2017: Journal of Lasers in Medical Sciences
https://www.readbyqxmd.com/read/29121359/single-spot-yellow-laser-versus-conventional-green-laser-on-panretinal-photocoagulation-patient-pain-scores-and-preferences
#14
Gerardo González-Saldivar, Sergio Rojas-Juárez, Itzel Espinosa-Soto, Jorge Sánchez-Ramos, Noel Jaurieta-Hinojosa, Abel Ramírez-Estudillo
BACKGROUND AND OBJECTIVE: Panretinal photocoagulation (PRP) is the mainstay therapy for proliferative diabetic retinopathy. Pain during and after its application is a complication that affects patients' therapeutic adherence. This study aimed to compare pain perception and patient preference for the 577-nm yellow laser (YL-577) (LIGHTL as 577; LIGHTMED, San Clemente, CA) and the conventional 532-nm green laser (GL-532) (Purepoint Laser; Alcon, Fort Worth, TX) with PRP. PATIENTS AND METHODS: A total of 92 patient eyes with proliferative diabetic retinopathy treated with PRP were randomly assigned to receive both GL-532 and YL-577 (184 eyes) - one on each eye, with the order of application randomized, as well...
November 1, 2017: Ophthalmic Surgery, Lasers & Imaging Retina
https://www.readbyqxmd.com/read/29120465/resonance-raman-detection-of-antioxidants-using-an-iron-oxide-nanoparticle-catalysed-decolourisation-assay
#15
Sian Sloan-Dennison, Neil C Shand, Duncan Graham, Karen Faulds
Nanozymes are metal nanoparticles with catalytic properties that can be used to oxidise peroxidase substrates giving a colorimetric response which can be detected using UV-vis, and recently, Raman spectroscopy. Due to their ease of synthesis and increased stability, nanozymes are being increasing investigated to replace conventional enzymes for the detection of biomolecules. Here we exploit the catalytic activity of iron oxide (Fe2O3) nanoparticles combined with the substrate 2,2-azinobis(3-ethyl-benzothiazoline-6-sulfonic acid) (ABTS) in a decolourisation assay for the detection of antioxidants...
November 9, 2017: Analyst
https://www.readbyqxmd.com/read/29119162/intense-red-up-conversion-luminescence-and-dynamical-processes-observed-in-sc2o3-yb-3-er-3-nanostructures
#16
Junxia Jiu, Xitao An, Jing Li, Jing Leng, Wei Lü, Li Chen, Xiaojun Wang
The intense red up-conversion luminescence in Sc2O3:Yb(3+),Er(3+) synthesized using a biphasic solvothermal (ST) method was observed upon laser diode pumping at 980 nm. Compared with that found in the bulk sample synthesized using a solid state (SS) reaction at the same sintering temperature (700 °C), the green and red up-conversion luminescence are enhanced by a factor of 19.7 and 23.4, respectively. The relative red intensity of the sample prepared using the ST method at 700 °C for 2 h was enhanced up to 6...
November 9, 2017: Dalton Transactions: An International Journal of Inorganic Chemistry
https://www.readbyqxmd.com/read/29115380/combination-treatment-of-adipose-derived-stem-cells-and-adiponectin-attenuates-pulmonary-arterial-hypertension-in-rats-by-inhibiting-pulmonary-arterial-smooth-muscle-cell-proliferation-and-regulating-the-ampk-bmp-smad-pathway
#17
Li Luo, Wuhong Zheng, Guili Lian, Huaning Chen, Ling Li, Changsheng Xu, Liangdi Xie
The present study aimed to assess the effects of therapy with adiponectin (APN) gene-modified adipose-derived stem cells (ADSCs) on pulmonary arterial hypertension (PAH) in rats and the underlying cellular and molecular mechanisms. ADSCs were successfully isolated from the rats and characterized. ADSCs were effectively infected with the green fluorescent protein (GFP)-empty (ADSCs-V) or the APN-GFP (ADSCs-APN) lentivirus and the APN expression was evaluated by ELISA. Sprague-Dawley rats were administered monocrotaline (MCT) to develop PAH...
October 31, 2017: International Journal of Molecular Medicine
https://www.readbyqxmd.com/read/29112179/green-ultrasound-versus-conventional-synthesis-and-characterization-of-specific-task-pyridinium-ionic-liquid-hydrazones-tethering-fluorinated-counter-anions-novel-inhibitors-of-fungal-ergosterol-biosynthesis
#18
Nadjet Rezki, Salsabeel A Al-Sodies, Sheikh Shreaz, Rayees Ahmad Shiekh, Mouslim Messali, Vaseem Raja, Mohamed R Aouad
A series of specific task ionic liquids (ILs) based on a pyridiniumhydrazone scaffold in combination with hexafluorophosphate (PF₆(-)), tetrafluoroboron (BF₄(-)) and/or trifluoroacetate (CF₃COO(-)) counter anion, were designed and characterized by IR, NMR and mass spectrometry. The reactions were conducted under both conventional and green ultrasound procedures. The antifungal potential of the synthesized compounds 2-25 was investigated against 40 strains of Candida (four standard and 36 clinical isolates)...
November 7, 2017: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29108577/functionalization-of-mesoporous-organosilica-nanocarrier-for-ph-glutathione-dual-responsive-drug-delivery-and-imaging-of-cancer-therapy-process
#19
Lin-Lin Hu, Jie Meng, Dan-Dan Zhang, Ming-Li Chen, Yang Shu, Jian-Hua Wang
A multifunctional drug nanocarrier is developed by incorporating acetaldehyde-modified-cystine (AMC) into mesoporous organosilica nanoparticles (MONs), shortly termed as MONs-AMC. The anticancer drug doxorubicin (DOX) links directly to MONs-AMC through electrostatic interaction between DOX and AMC to produce a conjugate, MONs-AMC-DOX, with a drug loading efficiency of 26.24 ± 1.35%, corresponding to a loading capacity of 0.26 ± 0.01mgmg(-1) for DOX. Schiff base AMC contains a -S-S- bond and two -C˭N- bonds which cleave in the presence of certain level of GSH and in an acidic medium, providing MONs-AMC-DOX the capability for triggering pH and glutathione (GSH) dual-responsive drug release...
January 15, 2018: Talanta
https://www.readbyqxmd.com/read/29105094/expression-of-tomato-prosystemin-gene-in-arabidopsis-reveals-systemic-translocation-of-its-mrna-and-confers-necrotrophic-fungal-resistance
#20
Haiyan Zhang, Pengli Yu, Jiuhai Zhao, Hongling Jiang, Haiyang Wang, Yingfang Zhu, Miguel A Botella, Jozef Šamaj, Chuanyou Li, Jinxing Lin
Systemin (SYS), an octadecapeptide hormone processed from a 200-amino-acid precursor (prosystemin, PS), plays a central role in the systemic activation of defense genes in tomato in response to herbivore and pathogen attacks. However, whether PS mRNA is transferable and its role in systemic defense responses remain unknown. We created the transgenic tomato PS gene tagged with the green fluorescent protein (PS-GFP) using a shoot- or root-specific promoter, and the constitutive 35S promoter in Arabidopsis. Subcellular localization of PS-/SYS-GFP was observed using confocal laser scanning microscopy and gene transcripts were determined using quantitative real-time PCR...
November 3, 2017: New Phytologist
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