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https://www.readbyqxmd.com/read/28878330/the-impact-of-wavelengths-of-led-light-therapy-on-endothelial-cells
#1
Sabrina Rohringer, Wolfgang Holnthoner, Sidrah Chaudary, Paul Slezak, Eleni Priglinger, Martin Strassl, Karoline Pill, Severin Mühleder, Heinz Redl, Peter Dungel
Low level light therapy receives increasing interest in the fields of tissue regeneration and wound healing. Several in vivo studies demonstrated the positive effects of LLLT on angiogenesis. This study aimed to investigate the underlying properties in vitro by comparing the effects of light therapy by light emitting diodes of different wavelengths on endothelial cells in vitro. Human umbilical vein endothelial cells were treated with either 475 nm, 516 nm or 635 nm light. Control cells were not illuminated...
September 6, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28663895/optimization-of-fluorescent-imaging-in-the-operating-room-through-pulsed-acquisition-and-gating-to-ambient-background-cycling
#2
Kristian J Sexton, Yan Zhao, Scott C Davis, Shudong Jiang, Brian W Pogue
The design of fluorescence imaging instruments for surgical guidance is rapidly evolving, and a key issue is to efficiently capture signals with high ambient room lighting. Here, we introduce a novel time-gated approach to fluorescence imaging synchronizing acquisition to the 120 Hz light of the room, with pulsed LED excitation and gated ICCD detection. It is shown that under bright ambient room light this technique allows for the detection of physiologically relevant nanomolar fluorophore concentrations, and in particular reduces the light fluctuations present from the room lights, making low concentration measurements more reliable...
May 1, 2017: Biomedical Optics Express
https://www.readbyqxmd.com/read/28628198/pulse-frequency-and-soil-litter-mixing-alter-the-control-of-cumulative-precipitation-over-litter-decomposition
#3
François-Xavier Joly, Kelsey L Kurupas, Heather L Throop
Macroclimate has traditionally been considered the predominant driver of litter decomposition. However, in drylands, cumulative monthly or annual precipitation typically fails to predict decomposition. In these systems, the windows of opportunity for decomposer activity may rather depend on the precipitation frequency and local factors affecting litter desiccation, such as soil-litter mixing. We used a full-factorial microcosm experiment to disentangle the relative importance of cumulative precipitation, pulse frequency, and soil-litter mixing on litter decomposition...
June 19, 2017: Ecology
https://www.readbyqxmd.com/read/28623354/tomographic-particle-image-velocimetry-using-smartphones-and-colored-shadows
#4
Andres A Aguirre-Pablo, Meshal K Alarfaj, Er Qiang Li, J F Hernández-Sánchez, Sigurdur T Thoroddsen
We demonstrate the viability of using four low-cost smartphone cameras to perform Tomographic PIV. We use colored shadows to imprint two or three different time-steps on the same image. The back-lighting is accomplished with three sets of differently-colored pulsed LEDs. Each set of Red, Green & Blue LEDs is shone on a diffuser screen facing each of the cameras. We thereby record the RGB-colored shadows of opaque suspended particles, rather than the conventionally used scattered light. We subsequently separate the RGB color channels, to represent the separate times, with preprocessing to minimize noise and cross-talk...
June 16, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28618783/pulsed-light-emitting-diodes-for-a-higher-phytochemical-level-in-microgreens
#5
Viktorija Vaštakaitė, Akvilė Viršilė, Aušra Brazaitytė, Giedrė Samuolienė, Julė Jankauskienė, Algirdas Novičkovas, Pavelas Duchovskis
A novel research of pulsed light-emitting diode (LED) lighting versus continuous lighting was conducted by analyzing phytochemical levels in microgreens. Red pak choi (Brassica rapa var. chinensis), mustard (Brassica juncea L.), and tatsoi (Brassica rapa var. rosularis) were grown indoors under HPS lamps supplemented with monochromatic (455, 470, 505, 590, and 627 nm) LEDs [total photosynthetic photon flux density (PPFD) of 200 ± 10 μmol m(-2) s(-1), for 16 h day(-1)]. For pulsed light treatments, the frequencies at 2, 32, 256, and 1024 Hz with a duty cycle of 50% monochromatic LEDs were applied...
August 9, 2017: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/28590222/phototaxis-of-propsilocerus-akamusi-diptera-chironomidae-from-a-shallow-eutrophic-lake-in-response-to-led-lamps
#6
Kimio Hirabayashi, Yoshinari Nagai, Tetsuya Mushya, Makoto Higashino, Yoshio Taniguchi
A study on the attraction of adult Propsilocerus akamusi midges to different-colored light traps was carried out from October 21 to November 15, 2013. The 6 colored lights used in light-emitting diode (LED) lamps were white, green, red, blue, amber, and ultraviolet (UV). The UV lamp attracted the most P. akamusi, followed by green, white, blue, amber, and red. A white pulsed LED light attracted only half the number of midges as did a continuous-emission white LED light. The result indicated that manipulation of light color, considering that the red LED light and/or pulsed LED light are not as attractive as the other colors, may be appropriate for the development of an overall integrated strategy to control nuisance P...
June 2017: Journal of the American Mosquito Control Association
https://www.readbyqxmd.com/read/28575999/sterilization-of-silicone-based-hydrogels-for-biomedical-application-using-ozone-gas-comparison-with-conventional-techniques
#7
Raquel Galante, Daniela Ghisleni, Patrizia Paradiso, Vitor D Alves, Terezinha J A Pinto, Rogério Colaço, Ana Paula Serro
Sterilization of hydrogels is challenging due to their often reported sensitivity to conventional methods involving heat or radiation. Although aseptic manufacturing is a possibility, terminal sterilization is safer in biological terms, leading to a higher overall efficiency, and thus should be used whenever it is possible. The main goal of this work was to study the applicability of an innovative ozone gas terminal sterilization method for silicone-based hydrogels and compare its efficacy and effects with those of traditional sterilization methods: steam heat and gamma irradiation...
September 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
https://www.readbyqxmd.com/read/28502048/the-effects-of-cadmium-pulse-dosing-on-physiological-traits-and-growth-of-the-submerged-macrophyte-vallisneria-spinulosa-and-phytoplankton-biomass-a-mesocosm-study
#8
Hui Liu, Yu Cao, Wei Li, Zhao Zhang, Erik Jeppesen, Wei Wang
Pulse inputs of heavy metals are expected to increase with a higher frequency of extreme climate events (heavy rain), leading to stronger erosion of contaminated and fertilized farmland soils to freshwaters, with potentially adverse effects on lake ecosystems. We conducted a 5-month mesocosm study to elucidate the responses of the submerged macrophyte Vallisneria spinulosa and phytoplankton to four different doses of cadmium (Cd): 0 (control), 0.05, 0.5, and 5 g m(-2) (CK, I, II, and III, respectively) under mesotrophic conditions...
June 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28362405/local-field-fluorescence-microscopy-imaging-cellular-signals-in-intact-hearts
#9
Yuriana Aguilar-Sanchez, Diego Fainstein, Rafael Mejia-Alvarez, Ariel L Escobar
In the heart, molecular signaling studies are usually performed in isolated myocytes. However, many pathological situations such as ischemia and arrhythmias can only be fully understood at the whole organ level. Here, we present the spectroscopic technique of local field fluorescence microscopy (LFFM) that allows the measurement of cellular signals in the intact heart. The technique is based on a combination of a Langendorff perfused heart and optical fibers to record fluorescent signals. LFFM has various applications in the field of cardiovascular physiology to study the heart under normal and pathological conditions...
March 8, 2017: Journal of Visualized Experiments: JoVE
https://www.readbyqxmd.com/read/27368071/minimizing-photodecomposition-of-flavin-adenine-dinucleotide-fluorescence-by-the-use-of-pulsed-leds
#10
J Rösner, A Liotta, E A Angamo, C Spies, U Heinemann, R Kovács
Dynamic alterations in flavin adenine dinucleotide (FAD) fluorescence permit insight into energy metabolism-dependent changes of intramitochondrial redox potential. Monitoring FAD fluorescence in living tissue is impeded by photobleaching, restricting the length of microfluorimetric recordings. In addition, photodecomposition of these essential electron carriers negatively interferes with energy metabolism and viability of the biological specimen. Taking advantage of pulsed LED illumination, here we determined the optimal excitation settings giving the largest fluorescence yield with the lowest photobleaching and interference with metabolism in hippocampal brain slices...
July 1, 2016: Journal of Microscopy
https://www.readbyqxmd.com/read/27323597/mumps-myocarditis-a-forgotten-disease
#11
Sheraz Hussain, Mohammad Faizan Zahid, Arshalooz Jamila Rahman, Mehnaz Atiq Ahmed, Shahnaz Hamid Ibrahim
Mumps is an acute viral illness that follows a self-limiting course but up to 10% of cases have a complicated course with the involvement of other organ systems. Myocarditis is reported as a complication but the incidence has greatly fallen ever since the development of the mumps vaccine. A child presented to our department with parotid swelling and fever. Persistent tachycardia with irregular pulse led to further cardiac work up which showed decreased ejection fraction and raised serum cardiac enzymes, indicating myocardial damage...
January 2016: Journal of Ayub Medical College, Abbottabad: JAMC
https://www.readbyqxmd.com/read/26935762/controlling-s2-population-in-cyanine-dyes-using-shaped-femtosecond-pulses
#12
Muath Nairat, Arkaprabha Konar, Vadim V Lozovoy, Warren F Beck, G J Blanchard, Marcos Dantus
Fast population transfer from higher to lower excited states occurs via internal conversion (IC) and is the basis of Kasha's rule, which states that spontaneous emission takes place from the lowest excited state of the same multiplicity. Photonic control over IC is of interest because it would allow direct influence over intramolecular nonradiative decay processes occurring in condensed phase. Here we tracked the S2 and S1 fluorescence yield for different cyanine dyes in solution as a function of linear chirp...
March 24, 2016: Journal of Physical Chemistry. A
https://www.readbyqxmd.com/read/26567781/time-resolved-ultra-high-resolution-optical-coherence-tomography-for-real-time-monitoring-of-selective-retina-therapy
#13
Patrick Steiner, Andreas Ebneter, Lieselotte Erika Berger, Martin Zinkernagel, Boris Považay, Christoph Meier, Jens H Kowal, Carsten Framme, Ralf Brinkmann, Sebastian Wolf, Raphael Sznitman
PURPOSE: Selective retina therapy (SRT) is a novel treatment for retinal pathologies, solely targeting the RPE. During SRT, the detection of an immediate tissue reaction is challenging, as tissue effects remain limited to intracellular RPE photodisruption. Time-resolved ultra-high axial resolution optical coherence tomography (OCT) is thus evaluated for the monitoring of dynamic optical changes at and around the RPE during SRT. METHODS: An experimental OCT system with an ultra-high axial resolution of 1...
October 2015: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/26375094/inf-ir-14-pulsed-led-light-does-not-affect-inflammatory-response-in-leukocytes
#14
S Chaudray, J Zipperle, A Islam, M Nimmervoll, L Karner, H Redl, S Bahrami, P Dungel
INTRODUCTION: Low-level light therapy (LLLT) is clinically used to attenuate inflammation and improve tissue repair processes. We aimed to evaluate the effects of red LED light on inflammatory response in a whole blood in vitro model as well as in isolated granulocytes. METHODS: Human whole blood was stimulated with 1ng/ml LPS for 4 h. Pulsed LED light (632 nm) was applied either 1 h or 4 h after LPS for 10 min. Granulocytes were stimulated with PMA or fMLP respectively, and treated with LED-light likewise...
October 2015: Shock
https://www.readbyqxmd.com/read/26152764/the-impact-of-delivery-profile-of-essential-amino-acids-upon-skeletal-muscle-protein-synthesis-in-older-men-clinical-efficacy-of-pulse-vs-bolus-supply
#15
COMPARATIVE STUDY
W Kyle Mitchell, Bethan E Phillips, John P Williams, Debbie Rankin, Jonathan N Lund, Daniel J Wilkinson, Kenneth Smith, Philip J Atherton
Essential amino acids (EAA) are responsible for skeletal muscle anabolic effects after nutrient intake. The pattern of appearance of EAA in blood, e.g., after intake of "slow" or "fast" protein sources or in response to grazing vs. bolus feeding patterns, may impact anabolism. However, the influence of this on muscle anabolism is poorly understood, particularly in older individuals. We determined the effects of divergent feeding profiles of EAA on blood flow, anabolic signaling, and muscle protein synthesis (MPS) in older men...
September 1, 2015: American Journal of Physiology. Endocrinology and Metabolism
https://www.readbyqxmd.com/read/26034817/gated-luminescence-imaging-of-silicon-nanoparticles
#16
Jinmyoung Joo, Xiangyou Liu, Venkata Ramana Kotamraju, Erkki Ruoslahti, Yoonkey Nam, Michael J Sailor
The luminescence lifetime of nanocrystalline silicon is typically on the order of microseconds, significantly longer than the nanosecond lifetimes exhibited by fluorescent molecules naturally present in cells and tissues. Time-gated imaging, where the image is acquired at a time after termination of an excitation pulse, allows discrimination of a silicon nanoparticle probe from these endogenous signals. Because of the microsecond time scale for silicon emission, time-gated imaging is relatively simple to implement for this biocompatible and nontoxic probe...
June 23, 2015: ACS Nano
https://www.readbyqxmd.com/read/25490664/revisiting-of-led-pumped-bulk-laser-first-demonstration-of-nd-yvo%C3%A2-led-pumped-laser
#17
Adrien Barbet, François Balembois, Amandine Paul, Jean-Philippe Blanchot, Anne-Lise Viotti, Jacques Sabater, Frédéric Druon, Patrick Georges
We describe here what is, to the best of our knowledge, the first LED pumped Nd:YVO₄ laser. Near-IR LED arrays with a wavelength centered close to 850 nm were used to pump transversely the crystal. By pulsing LEDs, with a duration of the order of the laser transition lifetime, we obtained sufficient pump intensities to reach the laser threshold. At a frequency of 250 Hz, we obtained an output energy of 40 μJ at 1064 nm for an input pump energy of 7.4 mJ, which corresponds to an optical efficiency of 0.5%...
December 1, 2014: Optics Letters
https://www.readbyqxmd.com/read/25167374/pulsed-electric-fields-for-burn-wound-disinfection-in-a-murine-model
#18
Alexander Golberg, G Felix Broelsch, Daniela Vecchio, Saiqa Khan, Michael R Hamblin, William G Austen, Robert L Sheridan, Martin L Yarmush
Emerging bacterial resistance renders many antibiotics ineffective, making alternative strategies of wound disinfection important. Here the authors report on a new, physical burn wound disinfection method: pulsed electric fields (PEFs). High voltage, short PEFs create nonthermal, permanent damage to cell membranes, possibly by irreversible electroporation. In medicine, PEF technology has recently been used for nonthermal ablation of solid tumors. The authors have expanded the spectrum of PEF applications in medicine to burn wound disinfection...
January 2015: Journal of Burn Care & Research: Official Publication of the American Burn Association
https://www.readbyqxmd.com/read/23961396/non-conventional-induction-strategies-for-production-of-subunit-swine-erysipelas-vaccine-antigen-in-re-coli-fed-batch-cultures
#19
Adilson José da Silva, Antônio Carlos Luperni Horta, Ana Maria Velez, Mônica Rosas C Iemma, Cíntia Regina Sargo, Raquel Lc Giordano, Maria Teresa M Novo, Roberto C Giordano, Teresa Cristina Zangirolami
In spite of the large number of reports on fed-batch cultivation of E. coli, alternative cultivation/induction strategies remain to be more deeply exploited. Among these strategies, it could be mentioned the use of complex media with combination of different carbon sources, novel induction procedures and feed flow rate control matching the actual cell growth rate. Here, four different carbon source combinations (glucose, glycerol, glucose + glycerol and auto-induction) in batch media formulation were compared...
2013: SpringerPlus
https://www.readbyqxmd.com/read/23868108/long-term-remission-of-atypical-hus-with-anti-factor-h-antibodies-after-cyclophosphamide-pulses
#20
Gwenaëlle Sana, Marie-Agnès Dragon-Durey, Marina Charbit, Karim Bouchireb, Caroline Rousset-Rouvière, Etienne Bérard, Rémi Salomon, Véronique Frémeaux-Bacchi, Patrick Niaudet, Olivia Boyer
BACKGROUND: Anti-complement factor H (CFH) autoantibody (Ab)-associated atypical hemolytic uremic syndrome (aHUS) has a poor prognosis, but no consensus exists on its treatment. METHODS: We report the follow-up of four children with anti-CFH Ab (8,000 to >32,000 arbitrary units)-associated aHUS after plasma exchanges (PEs), prednisone, and cyclophosphamide pulse therapy with the evolution of anti-CFH Ab titers and kidney function. RESULTS: Patient 1 received PEs + prednisone + cyclophosphamide pulses after two relapses following PEs and then PEs + rituximab...
January 2014: Pediatric Nephrology: Journal of the International Pediatric Nephrology Association
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