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https://www.readbyqxmd.com/read/29140068/arginine-rich-peptide-induced-supramolecular-self-assembly-of-water-soluble-anionic-alkynylplatinum-ii-complexes-a-continuous-and-label-free-luminescence-assay-for-trypsin-and-inhibitor-screening
#1
Angela Sin-Yee Law, Margaret Ching-Lam Yeung, Vivian Wing-Wah Yam
A water-soluble anionic alkynylplatinum(II) 2,6-bis(benzimidazol-2'-yl)pyridine (bzimpy) complex has been strategically designed and synthesized to show supramolecular self-assembly with cationic arginine-rich peptides through unique noncovalent Pt(II)···Pt(II) and π-π stacking interactions. Upon introduction of trypsin, the arginine-rich peptides can be hydrolyzed into small fragments and deaggregation of the platinum(II) complex molecules is observed. The aggregation-deaggregation process has been probed by UV-vis absorption, emission, and resonance light scattering (RLS) studies...
November 15, 2017: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29138863/therapeutic-effect-of-ulinastatin-on-pulmonary-fibrosis-via-downregulation-of-tgf%C3%A2-%C3%AE-1-tnf%C3%A2-%C3%AE-and-nf%C3%A2-%C3%AE%C2%BAb
#2
Dejun Li, Hongsheng Ji, Bao Zhao, Chunyang Xu, Wenjun Xia, Lihui Han, Dongqing Yu, Yuanrong Ju, Changjun Jin
Pulmonary fibrosis is a chronic, progressive, lethal lung disease characterized by alveolar cell necrosis and dysplasia of interstitial fibrotic tissue, resulting in loss of lung function and eventual respiratory failure. Previously, glucocorticoid drugs were used to treat this lung disorder. However, positive responses were recorded in less than half of treated patients and the cytotoxicity caused by high dosage treatment is still a concern. The present study investigated whether ulinastatin, a typical urinary trypsin inhibitor that mitigates numerous inflammatory responses, could be a treatment option for lung fibrosis...
November 14, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/29138626/efficient-method-for-the-rapid-purification-of-nosema-ceranae-spores
#3
Dong-Jun Kim, Hwi-Geon Yun, In-Hui Kim, Won-Seok Gwak, Soo-Dong Woo
Nosema ceranae is an obligate intracellular fungal parasite that causes mortality in honey bees and enhances the susceptibility of honey bees to other pathogens. Efficient purification of Nosema spores from the midgut of infected honey bees is very important because Nosema is non-culturable and only seasonably available. To achieve a higher yield of spores from honey bees, in this study, we considered that the initial release of spores from the midgut tissues was the most critical step. The use of 2 mm beads along with enzymatic treatment with collagenase and trypsin enhanced the homogenization of tissues and the yield of released spores by approximately 2...
September 2017: Mycobiology
https://www.readbyqxmd.com/read/29136907/ultrasonic-based-membrane-aided-sample-preparation-of-urine-proteomes
#4
Jemmyson Romário Jesus, Hugo M Santos, H López-Fernández, Carlos Lodeiro, Marco Aurélio Zezzi Arruda, J L Capelo
A new ultrafast ultrasonic-based method for shotgun proteomics as well as label-free protein quantification in urine samples is developed. The method first separates the urine proteins using nitrocellulose-based membranes and then proteins are in-membrane digested using trypsin. The enzymatic digestion process is accelerated from overnight to four minutes using a sonoreactor ultrasonic device. Overall, the sample treatment pipeline comprising protein separation, digestion and identification is done in just 3h...
February 1, 2018: Talanta
https://www.readbyqxmd.com/read/29136377/quantifying-missing-phospho-proteome-regions-with-the-broad-specificity-protease-subtilisin
#5
Humberto Gonczarowska-Jorge, Stefan Loroch, Margherita Dell'Aica, Albert Sickmann, Andreas Roos, René Peiman Zahedi
Despite huge efforts to map the human proteome using mass spectrometry the overall sequence coverage achieved to date is still below 50%. Reasons for missing areas of the proteome comprise protease-resistant domains including the lack/excess of enzymatic cleavage sites, non-unique peptide sequences, impaired peptide ionization/separation and low expression levels. To access novel areas of the proteome the beneficial use of enzymes complementary to trypsin, such as Glu-C, Asp-N, Lys-N, Arg-C, LysArginase has been reported...
November 14, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/29134575/impact-of-intramuscular-administration-of-lipid-soluble-and-water-soluble-vehicles-into-regenerating-muscle-at-the-distinct-phases-of-skeletal-muscle-regeneration
#6
Ratchakrit Srikuea, Kanokwan Suhatcho
Interpretation on the effectiveness of potential substances to enhance skeletal muscle regeneration is difficult if an inappropriate vehicle is administered, since vehicle administration can directly enhance or suppress regenerative capacity. In the current study, intramuscular administration of lipid-soluble and water-soluble vehicles into regenerating muscle at the distinct phases of skeletal muscle regeneration (regenerative vs. remodeling) were investigated. Tested vehicles included lipid-soluble [olive oil, (0...
November 13, 2017: Journal of Physiological Sciences: JPS
https://www.readbyqxmd.com/read/29134108/host-and-bacterial-proteases-influence-biofilm-formation-and-virulence-in-a-murine-model-of-enterococcal-catheter-associated-urinary-tract-infection
#7
Wei Xu, Ana L Flores-Mireles, Zachary T Cusumano, Enzo Takagi, Scott J Hultgren, Michael G Caparon
Enterococcus faecalis is a leading causative agent of catheter-associated urinary tract infection (CAUTI), the most common hospital-acquired infection. Its ability to grow and form catheter biofilm is dependent upon host fibrinogen (Fg). Examined here are how bacterial and host proteases interact with Fg and contribute to virulence. Analysis of mutants affecting the two major secreted proteases of E. faecalis OG1RF (GelE, SprE) revealed that while the loss of either had no effect on virulence in a murine CAUTI model or for formation of Fg-dependent biofilm in urine, the loss of both resulted in CAUTI attenuation and defective biofilm formation...
2017: NPJ Biofilms and Microbiomes
https://www.readbyqxmd.com/read/29133118/functional-characterization-of-a-serine-protease-inhibitor-modulated-in-the-infection-of-the-aedes-aegypti-with-dengue-virus
#8
Tatiane Sanches Soares, Boris Luis Rodriguez Gonzalez, Ricardo José Soares Torquato, Francisco Jose Alves Lemos, André L Costa-da-Silva, Margareth de Lara Capurro Guimarães, Aparecida Sadae Tanaka
During feeding with blood meal, female Aedes aegypti can transmit infectious agents, such as dengue, yellow fever, chikungunya and Zika viruses. Dengue virus causes human mortality in tropical regions of the world, and there is no specific treatment or vaccine with maximum efficiency being used for these infections. In the vector-virus interaction, the production of several molecules is modulated by both mosquitoes and invading agents. However, little information is available about these molecules in the Ae...
November 10, 2017: Biochimie
https://www.readbyqxmd.com/read/29127014/structural-insights-and-binding-of-a-natural-ligand-succinic-acid-with-serine-and-cysteine-proteases
#9
R Manohar, N H V Kutumbarao, Raghavendra Sashi Krishna Nagampalli, D Velmurugan, K Gunasekaran
In the age of growing infectious diseases, there is a great demand for new inhibitors which can exhibit minimum side effects. Owing to the importance of proteases in life cycle and invasion, they have been projected as attractive targets for structure based drug designing against microbes including viruses. Here we report the inhibitory activity of a well known natural compound succinic acid against both serine and cysteine proteases. The ligand is found co-crystallized with Bovine pancreatic trypsin in one of our crystallization trials and the diffraction data up to1...
November 7, 2017: Biochemical and Biophysical Research Communications
https://www.readbyqxmd.com/read/29126465/facile-synthesis-of-magnetic-metal-organic-frameworks-for-highly-efficient-proteolytic-digestion-used-in-mass-spectrometry-based-proteomics
#10
Rui Zhai, Yifeng Yuan, Fenglong Jiao, Feiran Hao, Xiang Fang, Yangjun Zhang, Xiaohong Qian
Mass spectrometry (MS) based bottom-up strategy is now the first choice for proteomics analysis. In this process, highly efficient and complete enzymatic degradation of protein samples is extremely important to achieve in-depth protein coverage and high-throughput protein profiling. However, conventional in-solution digestion suffer from long digestion time and enzyme autolysis that limit the protein sample processing throughput and identification accuracy. Here, we developed a novel type of magnetic metal organic frameworks (MOFs)-based immobilized enzyme reactor, Fe3O4@DOTA-ZIF-90-trypsin...
November 22, 2017: Analytica Chimica Acta
https://www.readbyqxmd.com/read/29123261/a-new-panel-of-pancreatic-cancer-biomarkers-discovered-using-a-mass-spectrometry-based-pipeline
#11
Xiaohui Liu, Weimin Zheng, Wansheng Wang, Huali Shen, Linxiao Liu, Wenhui Lou, Xiaolin Wang, Pengyuan Yang
BACKGROUND: Pancreatic carcinoma (PC) is an aggressive malignancy that lacks strategies for early detection. This study aimed to develop a coherent, high-throughput and non-discriminatory pipeline for the novel clinical biomarker discovery of PC. METHODS: We combined mass spectrometry (MS)-intensive methods such as isobaric tags for relative and absolute quantitation with two-dimensional liquid chromatography-tandem mass spectrometry (iTRAQ-2DLC-MS/MS), 1D-targeted LC-MS/MS, prime MRM (P-MRM) and stable isotope dilution-based MRM (SID-MRM) to analyse serum samples from healthy people (normal control, NC), patients with benign diseases (BD) and PC patients to identify novel biomarkers of PC...
November 9, 2017: British Journal of Cancer
https://www.readbyqxmd.com/read/29122847/human-airway-trypsin-like-protease-exerts-potent-antifibrotic-action-in-pulmonary-fibrosis
#12
Awen Menou, Pauline Flajolet, JanWillem Duitman, Aurélien Justet, Sophie Moog, Madeleine Jaillet, Laure Tabèze, Brigitte Solhonne, Marc Garnier, Hervé Mal, Pierre Mordant, Yves Castier, Aurélie Cazes, Jean-Michel Sallenave, Arnaud A Mailleux, Bruno Crestani
Idiopathic pulmonary fibrosis (IPF) is characterized by the deposition of excessive extracellular matrix and the destruction of lung parenchyma, resulting from an aberrant wound-healing response. Although IPF is often associated with an imbalance in protease activity, the mechanisms underlying the sustained repair mechanisms are not fully understood. Here, we addressed the role of the recently identified, membrane-anchored serine protease human airway trypsin-like protease (HAT). In the present study, we show that both HAT expression and activity were up-regulated in human IPF specimens...
November 9, 2017: FASEB Journal: Official Publication of the Federation of American Societies for Experimental Biology
https://www.readbyqxmd.com/read/29120791/identification-of-novel-dipeptidyl-peptidase-iv-dpp-iv-inhibitory-peptides-in-camel-milk-protein-hydrolysates
#13
Alice B Nongonierma, Sara Paolella, Priti Mudgil, Sajid Maqsood, Richard J FitzGerald
Nine novel dipeptidyl peptidase IV (DPP-IV) inhibitory peptides (FLQY, FQLGASPY, ILDKEGIDY, ILELA, LLQLEAIR, LPVP, LQALHQGQIV, MPVQA and SPVVPF) were identified in camel milk proteins hydrolysed with trypsin. This was achieved using a sequential approach combining liquid chromatography tandem mass spectrometry (LC-MS/MS), qualitative/quantitative structure activity relationship (QSAR) and confirmatory studies with synthetic peptides. The most potent camel milk protein-derived DPP-IV inhibitory peptides, LPVP and MPVQA, had DPP-IV half maximal inhibitory concentrations (IC50) of 87...
April 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29120788/milk-authenticity-by-ion-trap-proteomics-following-multi-enzyme-digestion
#14
Donatella Nardiello, Anna Natale, Carmen Palermo, Maurizio Quinto, Diego Centonze
The practice of adding adulterating substances in milk in order to raise profits is unfortunately worldwide. In addition, higher priced milk, coming from minor dairy species, is often illegally integrated with the lower priced cow milk. The presence of species-specific proteins, different from those declared in label, may be a serious problem for people with allergies. The development of proper analytical methods is therefore essential to protect consumer benefits and product authenticity. In this study, a proteomic approach for the detection of adulteration processes of specific milks in mixtures is proposed...
April 1, 2018: Food Chemistry
https://www.readbyqxmd.com/read/29117594/histones-and-chymotrypsin-like-elastases-play-significant-roles-in-the-antimicrobial-activity-of-tongue-sole-neutrophil-extracellular-traps
#15
Li-Lian Wen, Ming-Li Zhao, Heng Chi, Li Sun
Neutrophil extracellular traps (NETs) are a form of extracellular antimicrobial structure of neutrophils observed in higher and lower vertebrates, the latter including the teleost fish tongue sole Cynoglossus semilaevis. However, the antimicrobial mechanism of fish NETs is unknown. In the present study, we examined the potential contribution of histones and elastases to the antibacterial effect of tongue sole NETs. For this purpose, two histones (CsH2B and CsH4) and two elastases (CsEla1 and CsEla2) of tongue sole were investigated...
November 5, 2017: Fish & Shellfish Immunology
https://www.readbyqxmd.com/read/29115133/pursuing-the-promise-of-enzymatic-enhancement-with-nanoparticle-assemblies
#16
James Nicholas Vranish, Mario G Ancona, Scott A Walper, Igor L Medintz
The growing emphasis on green chemistry, renewable resources, synthetic biology, regio-/stereospecific chemical transformations, and nanotechnology for providing new biological products and therapeutics is reinvigorating research into enzymatic catalysis. Although the promise is profound, many complex issues remain to be addressed before this effort will have a significant impact. Prime among these is to combat the degradation of enzymes frequently seen in ex vivo formats following immobilization to stabilize the enzymes for long-term application and to find ways of enhancing their activity...
November 8, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/29113966/cystic-fibrosis-newborn-screening-outcome-of-infants-with-normal-sweat-tests
#17
Claire Edmondson, Christopher Grime, Ammani Prasad, Jacqui Cowlard, Chinedu E C Nwokoro, Gary Ruiz, Colin Wallis, Ian M Balfour-Lynn
Newborn babies positively screened for cystic fibrosis (CF) (high serum immunoreactive trypsin (IRT) with DNA analysis) are referred for a diagnostic sweat test, which may be normal (sweat chloride <30 mmol/L). Unless two gene mutations are identified during Newborn screening (NBS), the babies are discharged from follow-up. We wished to check that none had subsequently developed symptoms suggestive of CF. We retrospectively reviewed patient notes and contacted general practitioners of all babies with a negative sweat test, conducted in one of the four paediatric specialist CF centres in London, over the first 6 years of screening in South East England...
November 7, 2017: Archives of Disease in Childhood
https://www.readbyqxmd.com/read/29113045/pilot-study-comparison-of-sourdough-wheat-bread-and-yeast-fermented-wheat-bread-in-individuals-with-wheat-sensitivity-and-irritable-bowel-syndrome
#18
Reijo Laatikainen, Jari Koskenpato, Sanna-Maria Hongisto, Jussi Loponen, Tuija Poussa, Xin Huang, Tuula Sontag-Strohm, Hanne Salmenkari, Riitta Korpela
Many patients suspect wheat as being a major trigger of their irritable bowel syndrome (IBS) symptoms. Our aim was to evaluate whether sourdough wheat bread baked without baking improvers and using a long dough fermentation time (>12 h), would result in lower quantities of alpha-amylase/trypsin inhibitors (ATIs) and Fermentable, Oligo-, Di-, Mono-saccharides and Polyols (FODMAPs), and would be better tolerated than yeast-fermented wheat bread for subjects with IBS who have a poor subjective tolerance to wheat...
November 4, 2017: Nutrients
https://www.readbyqxmd.com/read/29111437/a-new-reliable-transposable-and-cost-effective-assay-for-absolute-quantification-of-total-plasmatic-bevacizumab-by-lc-ms-ms-in-human-plasma-comparing-two-internal-standard-calibration-approaches
#19
Rachel Legeron, Fabien Xuereb, Stephane Chaignepain, Alain-Pierre Gadeau, Stephane Claverol, Jean-William Dupuy, Sarah Djabarouti, Thierry Couffinhal, Jean-Marie Schmitter, Domonique Breilh
The quantification of monoclonal antibodies (mAbs) such as bevacizumab, a recombinant humanized immunoglobulin G1 (hIgG1), in biological fluids, is an essential prerequisite to any pharmacokinetic preclinical and clinical study. To date, reference techniques used to quantify mAbs rely on enzyme-linked immunosorbent assay (ELISA) lacking specificity. Furthermore, the commercially available ELISA kit to quantify bevacizumab in human plasma only assesses the free fraction of the drug. However, the conditions of storage and analysis of plasma samples could alter the physiological equilibrium between the free, bound and partially bound forms of bevacizumab and this could result in over- or underestimation of drug concentration...
October 23, 2017: Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
https://www.readbyqxmd.com/read/29111303/in-vitro-characteristics-of-endothelial-cells-prepared-from-human-cerebral-arteriovenous-malformation-lesions-using-a-novel-method
#20
Q Hao, X L Chen, L Ma, X Ye, H Wang, T T Wang, Y Hu, Y L Zhao
BACKGROUND AND PURPOSE: The cerebral arteriovenous malformation (cAVM) is a usual and continually unaware reason of heamorrhage and seizure. It contains of feeder arteries, drain veins and abnormal vessel nets. However, pathologic mechanisms of the development of cAVM are unknown. The purpose of this study was to explore a novel protocol to isolate, culture and passage endothelial cells (ECs) from human cAVM lesions. METHODS: We developed a protocol for isolating and growing ECs from eight patients with cAVM...
October 27, 2017: Microvascular Research
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