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https://www.readbyqxmd.com/read/28211311/oleiagrimonas-citrea-sp-nov-a-marine-bacterium-isolated-from-tidal-flat-sediment-and-emended-description-of-the-genus-oleiagrimonas-fang-et-al-2015-and-oleiagrimonas-soli
#1
Sung-Hyun Yang, Hyun-Seok Seo, Chi Nam Seong, Kae Kyoung Kwon
A Gram-negative, aerobic, rod-shaped (1.8-2.3 μm  0.4-0.6 μm), and motile marine bacterium, designated as MEBiC09124T was isolated from tidal flat sediment of the Suncheon Bay, South Korea. The 16S rRNA gene sequence analysis revealed that strain MEBiC09124T showed high similarity with the Oleiagrimonas soli 3.5XT (96.7%). Growth was observed at 18-38 OC (optimum 30 OC), at pH 4.0-8.5 (optimum pH 7.5) and with 0-6 % (optimum 2.5%) NaCl. The predominant cellular fatty acids were iso-C15 : 0 (22.6%), iso-C16 : 0 (16...
February 3, 2017: International Journal of Systematic and Evolutionary Microbiology
https://www.readbyqxmd.com/read/28207780/analyzing-pepsin-degradation-assay-conditions-used-for-allergenicity-assessments-to-ensure-that-pepsin-susceptible-and-pepsin-resistant-dietary-proteins-are-distinguishable
#2
Rong Wang, Thomas C Edrington, S Bradley Storrs, Kathleen S Crowley, Jason M Ward, Thomas C Lee, Zi L Liu, Bin Li, Kevin C Glenn
The susceptibility of a dietary protein to proteolytic degradation by digestive enzymes, such as gastric pepsin, provides information on the likelihood of systemic exposure to a structurally intact and biologically active macromolecule, thus informing on the safety of proteins for human and animal consumption. Therefore, the purpose of standardized in vitro degradation studies that are performed during protein safety assessments is to distinguish whether proteins of interest are susceptible or resistant to pepsin degradation via a study design that enables study-to-study comparison...
2017: PloS One
https://www.readbyqxmd.com/read/28201963/inhibition-of-amyloid-like-fibril-formation-of-trypsin-by-red-wines
#3
Márta Kotormán, Phanindra Babu Kasi, László Halász, Attila Borics
The aim of the present study was to examine the potential role and applicability of dietary supplements in reducing the risk of development of amyloid diseases associated with the gastrointestinal tract, such as type II diabetes. Trypsin, a well-known serine protease was used as a model protein in our experiments. The effect of various red wines on the formation of amyloid-like fibrils of trypsin was studied in vitro, in aqueous ethanol, at pH 7.0. Turbidity measurements, aggregation kinetics experiments, Congo red binding assays and electronic circular dichroism spectroscopic measurements were used to follow the aggregation process in the presence or absence of various red wines...
February 14, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28197985/inonotus-obliquus-polysaccharide-regulates-gut-microbiota-of-chronic-pancreatitis-in-mice
#4
Yang Hu, Chunying Teng, Sumei Yu, Xin Wang, Jinsong Liang, Xin Bai, Liying Dong, Tao Song, Min Yu, Juanjuan Qu
Polysaccharide is efficient in attenuation of metabolic ailments and modulation of gut microbiota as prebiotics. The therapeutic effect of Inonotus obliquus polysaccharide (IOP) on chronic pancreatitis (CP) in mice has been validated in our previous study. However, it is not clear whether IOP is conducive to maintaining the homeostasis between gut microbiota and host. The aim of this study is to testify the potential effects of IOP on gut microbiota composition and diversity in mice with CP. The changes in glutathione peroxidase (GSH-PX), total antioxidant capacity (TAOC), tumor necrosis factor alpha (TNF-α), transforming growth factor beta (TGF-β), lipase and trypsin levels were measured by commercial assay kits, meanwhile the gut microbiota composition and diversity were analyzed by high throughput sequencing...
December 2017: AMB Express
https://www.readbyqxmd.com/read/28196712/ipomoea-pes-caprae-l-r-br-convolvulaceae-as-a-topical-herbal-medicine-to-relieve-pain-and-inflammation-promising-tool-against-effects-due-to-toxin-skin-contact
#5
Cristiane da Silva Barth, Hugo Guilherme Tolentino de Souza, Lilian W Rocha, Gislaine Francieli da Silva, Mariana Ferreira Dos Anjos, Veronica D'Avila Pastor, Tania Mari Belle Bresolin, Angelica Garcia Couto, José Roberto Santin, Nara Lins Meira Quintão
ETHNOPHARMACOLOGICAL RELEVANCE: Ipomoea pes-caprae is known as bayhops, beach morning glory or goat's foot, and in Brazil as salsa-de-praia. Its leaves are used in worldwide folk medicine for the relief of jellyfish-stings symptoms. The literature only reports the anti-inflammatory and neutralizing effects of a nonpolar plant derived, without validating the popular use or exploring the mechanism of action. AIM OF THE STUDY: This study aimed to evaluate and validate the topical effects of a semisolid containing hydroethanolic extract obtained from the aerial parts of I...
February 11, 2017: Journal of Ethnopharmacology
https://www.readbyqxmd.com/read/28196225/blood-vessel-basement-membrane-alterations-in-human-retinal-microaneurysms-during-aging
#6
Mariana López-Luppo, Victor Nacher, David Ramos, Joana Catita, Marc Navarro, Ana Carretero, Alfonso Rodriguez-Baeza, Luísa Mendes-Jorge, Jesús Ruberte
Purpose: Microaneurysms, considered a hallmark of retinal vascular disease, are present in aged retinas. Here, the basement membrane of human retinal microaneurysms has been analyzed during aging. Methods: Retinas were obtained from 17 nondiabetic donors. Whole mount retinas and paraffin sections were marked immunohistochemically with antibodies against the main components of the blood basement membrane. Trypsin digestion and transmission electron microscopy also were performed...
February 1, 2017: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/28195949/a-preclinical-translational-study-of-the-cardioprotective-effects-of-plasma-derived-alpha-1-anti-trypsin-in-acute-myocardial-infarction
#7
Adolfo G Mauro, Eleonora Mezzaroma, Carlo Marchetti, Pratyush Narayan, Marco Del Buono, Marialessia Capuano, Andrea Prestamburgo, Simone Catapano, Fadi N Salloum, Antonio Abbate, Stefano Toldo
BACKGROUND: The area of myocardial infarction continues to expand for hours after reperfusion. The injured but viable myocardium may be salvaged if the signals leading to cell death are interrupted. Activation of the caspase-1 inflammasome in the heart shortly after ischemia-reperfusion contributes to the final infarct size. Plasma derived α-1 anti-trypsin (AAT) has shown to inhibit inflammasome formation in vitro and in vivo. To explore the potential translational clinical value of AAT as a therapeutic, we conducted a series of preclinical experiments designed to simulate clinically relevant scenarios...
February 13, 2017: Journal of Cardiovascular Pharmacology
https://www.readbyqxmd.com/read/28195578/specific-membrane-capacitance-cytoplasm-conductivity-and-instantaneous-young-s-modulus-of-single-tumour-cells
#8
Ke Wang, Yang Zhao, Deyong Chen, Beiyuan Fan, Yulan Lu, Lianhong Chen, Rong Long, Junbo Wang, Jian Chen
As label-free biomarkers, biophysical properties of cells are widely used for cell type classification. However, intrinsic biophysical markers, e.g., specific membrane capacitance (Cspecific membrane), cytoplasm conductivity (σconductivity) and instantaneous Young's modulus (Einstantaneous) measured for hundreds of single cells were not yet reported. In this study, single cells in suspension (adherent cells treated with trypsin) were aspirated through a microfluidic constriction channel at 25 °C, and the entry processes and impedance profiles were recorded and translated to Cspecific membrane, σconductivity and Einstantaneous...
February 14, 2017: Scientific Data
https://www.readbyqxmd.com/read/28194847/enzyme-initiated-free-radical-polymerization-of-molecularly-imprinted-polymer-nanogels-on-a-solid-phase-with-an-immobilized-radical-source
#9
Mira Daoud Attieh, Yi Zhao, Assem Elkak, Aude Falcimaigne-Cordin, Karsten Haupt
An enzyme-mediated synthetic approach is described for the preparation of molecularly imprinted polymer nanoparticles (MIP-NPs) in aqueous media. Horseradish peroxidase (HRP) was used to initiate the polymerization of methacrylate or vinyl monomers and cross-linkers by catalyzing the generation of free radicals. To prevent entrapment of the enzyme in the cross-linked polymer, and to enable it to be reused, HRP was immobilized on a solid support. MIPs based on 4-vinylpyridine and 1,4-bis(acryloyl)piperazine for the recognition of 2,4-dichlorophenoxyacetic acid (2,4-D) and salicylic acid were synthesized in an aqueous medium...
February 14, 2017: Angewandte Chemie
https://www.readbyqxmd.com/read/28193073/ultrasensitive-protease-sensors-using-selective-affinity-binding-selective-proteolytic-reaction-and-proximity-dependent-electrochemical-reaction
#10
Seonhwa Park, Gamwoo Kim, Jeongwook Seo, Haesik Yang
The development of a fast and ultrasensitive protease detection method is a challenging task. This paper reports ultrasensitive protease sensors exploiting (i) selective affinity binding, (ii) selective proteolytic reaction, and (iii) proximity-dependent electrochemical reaction. The selective affinity binding to capture IgG increases the concentration of the target protease (trypsin as a model protease) near the electrode, and the selective proteolytic reaction by trypsin increases the concentration of the redox-active species near the electrode...
December 20, 2016: Analytical Chemistry
https://www.readbyqxmd.com/read/28193007/enhanced-spatially-resolved-proteomics-using-on-tissue-hydrogel-mediated-protein-digestion
#11
David G Rizzo, Boone M Prentice, Jessica L Moore, Jeremy L Norris, Richard M Caprioli
The identification of proteins from tissue specimens is a challenging area of biological research. Many current techniques for identification forfeit some level of spatial information during the sample preparation process. Recently, hydrogel technologies have been developed that perform spatially localized protein extraction and digestion prior to downstream proteomic analysis. Regiospecific protein identifications acquired using this approach have thus far been limited to 1-2 mm diameter areas. The need to target smaller populations of cells with this technology necessitates the production of smaller diameter hydrogels...
February 17, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28192910/online-monitoring-of-enzymatic-reactions-using-time-resolved-desorption-electrospray-ionization-mass-spectrometry
#12
Si Cheng, Qiuhua Wu, He Xiao, Hao Chen
Electrospray ionization mass spectrometry (ESI-MS) is powerful for determining enzymatic reaction kinetics because of its soft ionization nature. However, it is limited to use ESI-favored solvents containing volatile buffers (e.g., ammonium acetate). In addition, lack of a quenching step for online ESI-MS reaction monitoring might introduce inaccuracy, due to the possible acceleration of reaction in the sprayed microdroplets. To overcome these issues, this study presents a new approach for online measuring enzymatic reaction kinetics using desorption electrospray ionization mass spectrometry (DESI-MS)...
February 9, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28192542/functional-diversity-of-secreted-cestode-kunitz-proteins-inhibition-of-serine-peptidases-and-blockade-of-cation-channels
#13
Martín Fló, Mariana Margenat, Leonardo Pellizza, Martín Graña, Rosario Durán, Adriana Báez, Emilio Salceda, Enrique Soto, Beatriz Alvarez, Cecilia Fernández
We previously reported a multigene family of monodomain Kunitz proteins from Echinococcus granulosus (EgKU-1-EgKU-8), and provided evidence that some EgKUs are secreted by larval worms to the host interface. In addition, functional studies and homology modeling suggested that, similar to monodomain Kunitz families present in animal venoms, the E. granulosus family could include peptidase inhibitors as well as channel blockers. Using enzyme kinetics and whole-cell patch-clamp, we now demonstrate that the EgKUs are indeed functionally diverse...
February 13, 2017: PLoS Pathogens
https://www.readbyqxmd.com/read/28191969/seekr-simulation-enabled-estimation-of-kinetic-rates-a-computational-tool-to-estimate-molecular-kinetics-and-its-application-to-trypsin-benzamidine-binding
#14
Lane William Votapka, Benjamin R Jagger, Alexandra L Heyneman, Rommie E Amaro
We present the Simulation Enabled Estimation of Kinetic Rates (SEEKR) package, a suite of open-source scripts and tools designed to enable researchers to perform multi-scale computation of the kinetics of molecular binding, unbinding, and transport using a combination of molecular dynamics, Brownian dynamics, and milestoning theory. To demonstrate its utility, we compute the kon, koff, and ∆Gbind for the protein trypsin with its noncovalent binder, benzamidine, and examine the kinetics and other results generated in the context of the new software, and compare our findings to previous studies performed on the same system...
February 13, 2017: Journal of Physical Chemistry. B
https://www.readbyqxmd.com/read/28187831/functional-analysis-of-five-trypsin-like-protease-genes-in-the-oriental-fruit-fly-bactrocera-dorsalis-diptera-tephritidae
#15
Ya-Li Li, Ming-Zhe Hou, Guang-Mao Shen, Xue-Ping Lu, Zhe Wang, Fu-Xian Jia, Jin-Jun Wang, Wei Dou
Insect midgut proteases catalyze the release of free amino acids from dietary proteins and are essential for insect normal development. To date, digestive proteases as potential candidates have made great progress in pest control. To clarify the function of trypsin-like protease genes in the digestive system of Bactrocera dorsalis, a serious pest of a wide range of tropical and subtropical fruit and vegetable crops, five trypsin genes (BdTry1, BdTry2, BdTry3, BdTry4 and BdTry5) were identified from transcriptome dataset, and the effects of feeding condition on their expression levels were examined subsequently...
March 2017: Pesticide Biochemistry and Physiology
https://www.readbyqxmd.com/read/28177773/gastroprotective-and-antielastase-effect-of-protein-inhibitors-from-erythrina-velutina-seeds-in-an-experimental-ulcer-model
#16
Vanessa Cristina Oliveira de Lima, Richele Janaína de Araújo Machado, Norberto Monteiro, Christina da Silva Camillo, Alexandre Coelho Serquiz, Adeliana Silva de Oliveira, Fabiola Rufino, Bruna Leal Lima Maciel, Adriana Ferreira Uchôa, Elizeu Antunes Santos, Ana Heloneida de Araújo Morais
Trypsin and chymotrypsin inhibitors from Erythrina velutina seeds have been previously isolated by our group. In previous studies using a sepsis model, we demonstrated the anti-tumor and anti-inflammatory action of these compounds. This study aimed to evaluate the gastroprotective and antielastase effect of protein inhibitors from Erythrina velutina seeds in an experimental stress induced ulcer model. Two protein isolates from E. velutina seeds, with antitrypsin (PIAT) and anti-chymotrypsin (PIAQ) activities were tested...
September 12, 2016: Biochemistry and Cell Biology, Biochimie et Biologie Cellulaire
https://www.readbyqxmd.com/read/28177574/characterization-of-protein-interactions-with-molecularly-imprinted-hydrogels-that-possess-engineered-affinity-for-high-isoelectric-point-biomarkers
#17
John R Clegg, Justin X Zhong, Afshan S Irani, Joann Gu, David S Spencer, Nicholas A Peppas
Molecularly imprinted polymers (MIPs) with selective affinity for protein biomarkers could find extensive utility as environmentally robust, cost-efficient biomaterials for diagnostic and therapeutic applications. In order to develop recognitive, synthetic biomaterials for prohibitively expensive protein biomarkers, we have developed a molecular imprinting technique that utilizes structurally similar, analogue proteins. Hydrogel microparticles synthesized by molecular imprinting with trypsin, lysozyme, and cytochrome c possessed an increased affinity for alternate high isoelectric point biomarkers both in isolation and plasma-mimicking adsorption conditions...
February 8, 2017: Journal of Biomedical Materials Research. Part A
https://www.readbyqxmd.com/read/28174513/cervical-mucus-proteome-in-endometriosis
#18
Giuseppe Grande, Federica Vincenzoni, Domenico Milardi, Giuseppina Pompa, Domenico Ricciardi, Erika Fruscella, Francesca Mancini, Alfredo Pontecorvi, Massimo Castagnola, Riccardo Marana
BACKGROUND: Endometriosis is a chronic gynecological inflammatory disease characterized by the presence of functional endometrial glands and stroma outside of the uterine cavity. It affects 7-10% of women of reproductive age and up to 50% of women with infertility. The current gold standard for the diagnosis combines laparoscopic evaluation and biopsy of the visualized lesions. However, laparoscopy requires general anesthesia and developed surgical skills and it has a high procedural cost...
2017: Clinical Proteomics
https://www.readbyqxmd.com/read/28171222/effect-of-long-term-cultivation-on-morphological-and-biological-characteristics-of-human-periodontal-ligament-stem-cells
#19
(no author information available yet)
OBJECTIVES: Recently, it was demonstrated that human periodontal ligament stem cells have great potential for tissue engineering and regenerative medicine not limited to oro-maxillofacial region. They are easily accessible and they may be expanded under in vitro conditions. In this study we assessed the effect of long-term cultivation on the selected biological and morphological properties of human periodontal ligament stem cells. METHODS: Periodontal tissues were obtained from normal impacted third molars of healthy donors (n=5; aged 18-27 years), after obtaining informed consent...
October 8, 2016: Neuro Endocrinology Letters
https://www.readbyqxmd.com/read/28168724/identification-of-fibulin-1-as-a-human-bone-marrow-stromal-hs-5-sell-derived-factor-that-induces-human-prostate-cancer-cell-death
#20
Kornkamon Lertsuwan, Leila H Choe, Irene R Marwa, Kelvin Lee, Robert A Sikes
BACKGROUND: Previous studies showed that human bone marrow stromal HS-5 cells secreted unidentified factor(s) inducing PCa cell death. Herein, the HS-5-derived factor (HS-5 DF) was characterized and identified. METHODS: Conditioned media from confluent HS-5 cells were collected and modified for biochemical characteristic testing of HS-5 DF. Cell survival was measured by apoptosis assay and live/dead assay. Fibulin-1 was identified from gel electrophoresis and mass spectrometry...
February 7, 2017: Prostate
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