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https://www.readbyqxmd.com/read/28932818/impact-of-cell-culture-parameters-on-production-and-vascularization-bioactivity-of-mesenchymal-stem-cell-derived-extracellular-vesicles
#1
Divya B Patel, Kelsey M Gray, Yasasvhinie Santharam, Tek N Lamichhane, Kimberly M Stroka, Steven M Jay
Mesenchymal stem cell (MSC)-derived extracellular vesicles (EVs) have emerged as potential therapeutic agents for numerous applications. EVs offer potential advantages over cell-based therapies with regard to safety, stability and clearance profiles, however production and potency limitations must be addressed to enable eventual translation of EV-based approaches. Thus, we sought to examine the role of specific cell culture parameters on MSC EV production and bioactivity toward informing rational design parameters for scalable EV biomanufacturing...
June 2017: Bioengineering & Translational Medicine
https://www.readbyqxmd.com/read/28930522/advances-in-tick-vaccinology-in-brazil-from-gene-expression-to-immunoprotection
#2
Renato Andreotti, Poliana Fernanda Giachetto, Rodrigo Casquero Cunha
Rhipicephalus (Boophilus) microplus has substantial economic impact on the cattle breeding industry and, chemical control and tick resistance development are the major concern. There is a worldwide search for new options, and control using vaccines has been the main focus nowadays. Studies performed in Brazil found that Bm86-based immunization of bovines reduced the infestation of R. (B.) microplus of vaccinated bovines by 45% to 60%. Native Boophilusmicroplus tripsin inhibitors (BmTIs) with trypsin-, kallikrein-, and elastase-inhibiting activities have been used as immunogens in bovines reaching 72...
January 1, 2018: Frontiers in Bioscience (Scholar Edition)
https://www.readbyqxmd.com/read/28926939/kempopeptin-c-a-novel-marine-derived-serine-protease-inhibitor-targeting-invasive-breast-cancer
#3
Fatma H Al-Awadhi, Lilibeth A Salvador, Brian K Law, Valerie J Paul, Hendrik Luesch
Kempopeptin C, a novel chlorinated analogue of kempopeptin B, was discovered from a marine cyanobacterium collected from Kemp Channel in Florida. The structure was elucidated using NMR spectroscopy and mass spectrometry (MS). The presence of the basic Lys residue adjacent to the N-terminus of the 3-amino-6-hydroxy-2-piperidone (Ahp) moiety contributed to its selectivity towards trypsin and related proteases. The antiproteolytic activity of kempopeptin C was evaluated against trypsin, plasmin and matriptase and found to inhibit these enzymes with IC50 values of 0...
September 16, 2017: Marine Drugs
https://www.readbyqxmd.com/read/28926141/working-at-the-membrane-interface-ligand-induced-changes-in-dynamic-conformation-and-oligomeric-structure-in-human-aromatase
#4
Giovanna Di Nardo, Giuseppe Cimicata, Roberta Baravalle, Valentina Dell'Angelo, Alberto Ciaramella, Gianluca Catucci, Piero Ugliengo, Gianfranco Gilardi
Aromatase catalyses the biosynthesis of estrogens from androgens. Due to the physiological importance of this conversion of lipophilic substrates, the interaction with the lipid bilayer for this cytochrome P450 is crucial for its dynamics that must allow an easy access to substrates and inhibitors. Here, aromatase-anastrozole interaction is studied combining computational methods to identify possible access/egress routes with the protein inserted in the membrane and experimental tools aimed at the investigation of the effect of the inhibitor on the protein conformation...
September 19, 2017: Biotechnology and Applied Biochemistry
https://www.readbyqxmd.com/read/28925864/kinetic-characterization-of-anticarsia-gemmatalis-digestive-serine-proteases-and-the-inhibitory-effect-of-synthetic-peptides
#5
Adriana Patarroyo Vargas, Yaremis Meriño Cabrera, José Cola Zanuncio, Francelina Rocha, Wellington Garcia Campos, Maria Goreti de Almeida Oliveira
The kinetics of Anticarsia gemmatalis Hübner gut serine proteases with tripeptidyl substrates, and the enzymatic inhibition model using synthetic peptides Gor 3, Gor 4, and Gor 5 are described. The enzymes were purified on p-aminobenzamidine agarose affinity column in an FPLC system using electrophoresis with 12.5% polyacrylamide gel. Michaelis-Menten constants and the inhibition model were determined according to the Dixon methodology and Lineweaver-Burk's double reciprocal. The KM values and catalytic constants of peptide substrates show that A...
September 17, 2017: Protein and Peptide Letters
https://www.readbyqxmd.com/read/28923071/characteristics-of-influenza-h13n8-subtype-virus-firstly-isolated-from-qinghai-lake-region-china
#6
Jie Dong, Hong Bo, Ye Zhang, Libo Dong, Shumei Zou, Weijuan Huang, Jia Liu, Dayan Wang, Yuelong Shu
BACKGROUND: Since the highly pathogenic H5N1 influenza caused thousands of deaths of wild bird in this area in 2005, Qinghai Lake in China has become a hot spot for study of the influence of avian influenza to migratory wild birds. However, the ecology and evolution of low pathogenic avian influenza virus in this region are limited. This project-based avian influenza surveillance in Qinghai lake region was initiated in year 2012. METHOD: Samples of wild bird feces and lake surface water were collected in Qinghai Lake in year 2012...
September 18, 2017: Virology Journal
https://www.readbyqxmd.com/read/28921955/characterization-of-site-specific-glycosylation-in-influenza-a-virus-hemagglutinin-produced-by-spodoptera-frugiperda-insect-cell-line
#7
Yanjun Liu, Shiaw-Lin Wu, Kerry Routenberg Love, William S Hancock
Influenza hemagglutinin is a surface glycoprotein related to virus invasion and host immune system response. Understanding site specific glycosylation of hemagglutinin will increase our knowledge about virus evolution and can improve the design and quality of vaccines. In our study, we used glycoproteomic analysis based on multienzyme digestion followed by LC tandem MS analysis to determine the glycosylation of Influenza hemagglutinin (H1/A/California/04/2009) using the following steps: PNGaseF treatment combined with trypsin or pepsin digestion were used to determine the glycosites and glycan occupancy...
September 18, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28920176/isolation-and-identification-of-antifungal-peptides-from-bacillus-amyloliquefaciens-w10
#8
Qing-Xia Zhang, Ying Zhang, Hai-Huan Shan, Yun-Hui Tong, Xi-Jun Chen, Feng-Quan Liu
Antifungal metabolites produced by Bacillus sp. W10, which was previously isolated from the tomato rhizosphere, were investigated. Strain W10 was identified as Bacillus amyloliquefaciens by analysis of its 16S rDNA and gyrB gene partial sequences. PCR analysis showed the presence of fenB, sfp, and ituD genes, coding for fengycin, surfactin, and iturin, respectively. A novel small antifungal peptide, designated 5240, produced by this strain was isolated by ammonium sulfate precipitation and Superdex 200 gel filtration chromatography...
September 17, 2017: Environmental Science and Pollution Research International
https://www.readbyqxmd.com/read/28920115/gold-nanodome-sers-platform-for-label-free-detection-of-protease-activity
#9
Pieter C Wuytens, Hans Demol, Nina Turk, Kris Gevaert, Andre G Skirtach, Mohamed Lamkanfi, Roel Baets
Surface-enhanced Raman scattering provides a promising technology for sensitive and selective detection of protease activity by monitoring peptide cleavage. Not only are peptides and plasmonic hotspots similarly sized, Raman fingerprints also hold large potential for spectral multiplexing. Here, we use a gold-nanodome platform for real-time detection of trypsin activity on a CALNNYGGGGVRGNF substrate peptide. First, we investigate the spectral changes upon cleavage through the SERS signal of liquid-chromatography separated products...
September 18, 2017: Faraday Discussions
https://www.readbyqxmd.com/read/28917871/inhibition-of-gelatinized-rice-starch-retrogradation-by-rice-bran-protein-hydrolysates
#10
Liya Niu, Leiyan Wu, Jianhui Xiao
The retrogradation of gelatinized rice starch (GRS) during the shelf life of a product is the biggest barrier related to starch-containing foods. The objective of this study was to produce rice bran protein hydrolysate (RBPH) using proteolytic enzymes (alcalase, flavourzyme, protamex, neutrase, bromelain, papain and trypsin) to suppress the retrogradation of GRS and understand the physical phenomena underlying the reduced retrogradation of GRS by RBPH during short- and long-term storage. Mixtures of GRS incorporated with Protamex-hydrolyzed rice bran protein at 1h (PRBPH-1) at a degree of hydrolysis of 15...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917852/bio-responsive-alginate-keratin-composite-nanogels-with-enhanced-drug-loading-efficiency-for-cancer-therapy
#11
Zhe Sun, Zeng Yi, Huaiying Zhang, Xiaomin Ma, Wen Su, Xiaoyu Sun, Xudong Li
This article presents a novel dual-stimuli responsive nanogel prepared from human hair keratin and alginate through simple crosslinking method. Keratin offer the crosslinking structure and bio-responsive ability and alginate ameliorated properties of nanogels including particle size, stability and drug loading capacity. The resultant keratin-alginate nanogels (KSA-NGs) could function as promising vectors for doxorubicin hydrochloride (DOX) with a super-high drug-loading rate of 52.9% (w/w) and dual-stimuli responsive behavior to GSH and trypsin...
November 1, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28917670/morphological-characterization-and-biocontrol-effects-of-vibrio-vulnificus-phages-against-vibriosis-in-the-shrimp-aquaculture-environment
#12
P Srinivasan, P Ramasamy
The re-emerging field of phage therapy is the potential biocontrol agents for the transfer of virulence factor and to kill their bacterial hosts. In this study, the lytic Vibrio vulnificus phages were studied to provide a better understanding of phage-host interactions and development of phage therapy. Four new V. vulnificus phages were detected from shrimp aquaculture system, named VV1, VV2, VV3 and VV4. All lytic V. vulnificus phages are the Tecviruses of the family Tectiviridae with typical double layered elongated icosahedral head and tailless morphology...
September 13, 2017: Microbial Pathogenesis
https://www.readbyqxmd.com/read/28917645/anti-fibrinolytic-and-anti-microbial-activities-of-a-serine-protease-inhibitor-from-honeybee-apis-cerana-venom
#13
Jie Yang, Kwang Sik Lee, Bo Yeon Kim, Yong Soo Choi, Hyung Joo Yoon, Jingming Jia, Byung Rae Jin
Bee venom contains a variety of peptide constituents, including low-molecular-weight protease inhibitors. While the putative low-molecular-weight serine protease inhibitor Api m 6 containing a trypsin inhibitor-like cysteine-rich domain was identified from honeybee (Apis mellifera) venom, no anti-fibrinolytic or anti-microbial roles for this inhibitor have been elucidated. In this study, we identified an Asiatic honeybee (A. cerana) venom serine protease inhibitor (AcVSPI) that was shown to act as a microbial serine protease inhibitor and plasmin inhibitor...
September 13, 2017: Comparative Biochemistry and Physiology. Toxicology & Pharmacology: CBP
https://www.readbyqxmd.com/read/28916914/the-effects-of-protein-and-fiber-content-on-gut-structure-and-function-in-zebrafish-danio-rerio
#14
Samantha C Leigh, Bao-Quang Nguyen-Phuc, Donovan P German
Chemical reactor theory (CRT) suggests that the digestive tract functions as a chemical reactor for processing food. Presumably, gut structure and function should match diet to ensure adequate nutrient and energy uptake to maintain performance. Within CRT, dietary biochemical composition is the most important factor affecting gut structure and function in vertebrates. We fed Danio rerio (zebrafish) diets ranging from high- to moderate- to low-quality (i.e., ranging from high-protein, low-fiber to low-protein, high-fiber), and observed how gut length and surface area, as well as the activity levels of digestive enzymes (amylase, maltase, trypsin, aminopeptidase, and lipase) shifted in response to these dietary changes...
September 15, 2017: Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
https://www.readbyqxmd.com/read/28913664/supplementation-of-enzyme-treated-soy-protein-saves-dietary-protein-and-promotes-digestive-and-absorptive-ability-referring-to-tor-signaling-in-juvenile-fish
#15
Weiwei Xiao, Weidan Jiang, Lin Feng, Yang Liu, Pei Wu, Jun Jiang, Yongan Zhang, Xiaoqiu Zhou
This study was conducted to evaluate the effect of enzyme-treated soy protein (ETSP) supplementation in the low-protein diet on growth performance, digestive and absorptive capacities, and related signaling molecules' gene expressions in juvenile Jian carp. The results showed that percent weight gain (PWG), specific growth rate (SGR), and feed intake (FI) were decreased by reducing dietary protein from 34 to 32% (P < 0.05). Supplying low-protein diet with optimal ETSP increased previously mentioned indices of juvenile Jian carp (P < 0...
September 14, 2017: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/28912721/physical-and-chemical-processes-and-the-morphofunctional-characteristics-of-human-erythrocytes-in-hyperglycaemia
#16
Victor V Revin, Natalia A Klenova, Natalia V Gromova, Igor P Grunyushkin, Ilia N Solomadin, Alexander Y Tychkov, Anastasia A Pestryakova, Anna V Sadykhova, Elvira S Revina, Ksenia V Prosnikova, Jean-Christophe Bourdon, Nikolai Zhelev
Background: This study examines the effect of graduated hyperglycaemia on the state and oxygen-binding ability of hemoglobin, the correlation of phospholipid fractions and their metabolites in the membrane, the activity of proteolytic enzymes and the morphofunctional state of erythrocytes. Methods: Conformational changes in the molecule of hemoglobin were determined by Raman spectroscopy. The structure of the erythrocytes was analyzed using laser interference microscopy (LIM). To determine the activity of NADN-methemoglobinreductase, we used the P...
2017: Frontiers in Physiology
https://www.readbyqxmd.com/read/28912505/biomimetic-silica-nanoparticles-prepared-by-a-combination-of-solid-phase-imprinting-and-ostwald-ripening
#17
Elena Piletska, Heersh Yawer, Francesco Canfarotta, Ewa Moczko, Katarzyna Smolinska-Kempisty, Stanislav S Piletsky, Antonio Guerreiro, Michael J Whitcombe, Sergey A Piletsky
Herein we describe the preparation of molecularly imprinted silica nanoparticles by Ostwald ripening in the presence of molecular templates immobilised on glass beads (the solid-phase). To achieve this, a seed material (12 nm diameter silica nanoparticles) was incubated in phosphate buffer in the presence of the solid-phase. Phosphate ions act as a catalyst in the ripening process which is driven by differences in surface energy between particles of different size, leading to the preferential growth of larger particles...
September 14, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28910348/the-effect-of-corn-trypsin-inhibitor-anti-tissue-factor-pathway-inhibitor-antibodies-and-phospholipids-on-microvesicle-associated-thrombin-generation-in-patients-with-pancreatic-cancer-and-healthy-controls
#18
Marit Hellum, Isabel Franco-Lie, Reidun Øvstebø, Truls Hauge, Carola E Henriksson
Circulating microvesicles (MVs) are suggested to be important contributors to cancer-associated thrombosis due to the presence of surface-bound procoagulant molecules like tissue factor (TF) and phosphatidylserine (PS). Pancreatic cancer is considered to be one of the most prothrombotic malignancies. The aim of this study was to describe the impact of analytical variables on MV-associated thrombin generation in patients with pancreatic cancer and in healthy controls. MVs were isolated from citrated plasma and added to pooled normal plasma (PNP)...
2017: PloS One
https://www.readbyqxmd.com/read/28904177/limited-proteolysis-as-a-tool-to-probe-the-tertiary-conformation-of-dysferlin-and-structural-consequences-of-patient-missense-variant-l344p
#19
Natalie Woolger, Adam Bournazos, Reece A Sophocleous, Frances J Evesson, Angela Lek, Birgit Driemer, R Bryan Sutton, Sandra T Cooper
Dysferlin is a large transmembrane protein that plays a key role in cell membrane repair, and underlies a recessive form of inherited muscular dystrophy. Dysferlinopathy is characterized by absence or marked reduction of dysferlin protein, with 43% of reported pathogenic variants being missense variants that span the length of the dysferlin protein. The unique structure of dysferlin, with seven tandem C2-domains separated by linkers, suggest dysferlin may dynamically associate with phospholipid membranes in response to Ca2+ signaling...
September 13, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28903902/single-cell-gene-variation-analysis-method-for-single-gland
#20
Yan Zhou, Chao-Jie Wang, Chun-Chao Zhu, Jiang-Rong Chen, Ming Cheng, Yu-Liang Deng, Yan Guo
Single-cell analysis of heterogeneity has become the cutting-edge technology for profound understandings of relationships between cell populations. At present, common methods used in single cellular genomic research are mainly microfluidic technologies (Fluidigm) or based on microwells, both requiring a uniform size of cells at the entrance. However, the size of cells in specific tissues can vary from type to type. To address this issue, we need to establish a method to identify genomic features of individual cells of different sizes...
August 20, 2017: Yi Chuan, Hereditas
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