keyword
MENU ▼
Read by QxMD icon Read
search

trypsin

keyword
https://www.readbyqxmd.com/read/29352619/quantitative-evaluation-of-enzyme-induced-porcine-articular-cartilage-degeneration-based-on-observation-of-entire-cartilage-layer-using-ultrasound
#1
Jingchen Zhang, Liying Xiao, Lingying Tong, Chao Wan, Zhixiu Hao
Enzyme-induced articular cartilage degeneration resembling osteoarthritis was evaluated using a newly defined acoustic parameter, the "averaged magnitude ratio" (AMR), which has been suggested as an indicator of articular cartilage degeneration. In vitro experiments were conducted on porcine cartilage samples digested with trypsin for 2 h (n = 10) and 4 h (n = 13) and healthy control samples (n = 13). AMR was determined with 15- and 25-MHz ultrasound, and the integrated reflection coefficient (IRC) and apparent integrated backscattering coefficient (AIB) were also calculated for comparison...
January 15, 2018: Ultrasound in Medicine & Biology
https://www.readbyqxmd.com/read/29352220/soce-induced-calcium-overload-regulates-autophagy-in-acute-pancreatitis-via-calcineurin-activation
#2
Zhen-Dong Zhu, Tao Yu, Hua-Jing Liu, Jing Jin, Jun He
Acute pancreatitis (AP) is an acute inflammatory process of the pancreas that is characterized by inflammation, edema, vacuolization and necrosis, which has significant morbidity and lethality. The pathogenesis of AP has not been established completely. An early and critical feature of AP is the aberrant signaling of Calcium (Ca2+) within the pancreatic acinar cell, termed Ca2+ overload. Store-operated Ca2+ (SOC) channels are the principal Ca2+ influx channels that contribute to Ca2+ overload in pancreatic acinar cells...
January 19, 2018: Cell Death & Disease
https://www.readbyqxmd.com/read/29351798/comparative-salivary-proteomics-analysis-of-children-with-and-without-dental-caries-using-the-itraq-mrm-approach
#3
Kun Wang, Yufei Wang, Xiuqing Wang, Qian Ren, Sili Han, Longjiang Ding, Zhongcheng Li, Xuedong Zhou, Wei Li, Linglin Zhang
BACKGROUND: Dental caries is a major worldwide oral disease afflicting a large proportion of children. As an important host factor of caries susceptibility, saliva plays a significant role in the occurrence and development of caries. The aim of the present study was to characterize the healthy and cariogenic salivary proteome and determine the changes in salivary protein expression of children with varying degrees of active caries, also to establish salivary proteome profiles with a potential therapeutic use against dental caries...
January 19, 2018: Journal of Translational Medicine
https://www.readbyqxmd.com/read/29350914/cys34-adductomes-differ-between-patients-with-chronic-lung-or-heart-disease-and-healthy-controls-in-central-london
#4
Sa Liu, Hasmik Grigoryan, William Matthew Bell Edmands, Sonia Dagnino, Rudy Sinharay, Paul Cullinan, Peter Collins, Kian Fan Chung, Benjamin M Barratt, Frank J Kelly, Paolo Vineis, Stephen M Rappaport
Oxidative stress generates reactive species that modify proteins, deplete antioxidant defenses and contribute to chronic obstructive pulmonary disease (COPD) and ischemic heart disease (IHD). To determine whether protein modifications differ between COPD or IHD patients and healthy subjects, we performed untargeted analysis of adducts at the Cys34 locus of human serum albumin (HSA). Biospecimens were obtained from nonsmoking participants from London, U.K., including healthy subjects (n=20) and patients with COPD (n=20) or IHD (n=10)...
January 19, 2018: Environmental Science & Technology
https://www.readbyqxmd.com/read/29350038/efficient-mass-spectral-analysis-of-active-transporters-overexpressed-in-escherichia-coli
#5
Mamiyo Kawakami, Narinobu Juge, Yuri Kato, Hiroshi Omote, Yoshinori Moriyama, Takaaki Miyaji
Structural analysis of purified active membrane proteins can be performed by mass spectrometry (MS). However, no large-scale expression systems for active eukaryotic membrane proteins are available. Moreover, because membrane proteins cannot easily be digested by trypsin and ionized, they are difficult to analyze by MS. Here, we developed a method for mass spectral analysis of eukaryotic membrane proteins combined to an overexpression system in Escherichia coli. Vesicular glutamate transporter 2 (VGLUT2/SLC17A6) with a soluble α-helical protein and histidine tag on the N- and C-terminus, respectively, was overexpressed in E...
January 19, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29346283/the-biological-role-of-hyaluronan-rich-oocyte-cumulus-extracellular-matrix-in-female-reproduction
#6
REVIEW
Eva Nagyova
Fertilization of the mammalian oocyte requires interactions between spermatozoa and expanded cumulus extracellular matrix (ECM) that surrounds the oocyte. This review focuses on key molecules that play an important role in the formation of the cumulus ECM, generated by the oocyte-cumulus complex. In particular, the specific inhibitors (AG1478, lapatinib, indomethacin and MG132) and progesterone receptor antagonist (RU486) exerting their effects through the remodeling of the ECM of the cumulus cells surrounding the oocyte have been described...
January 18, 2018: International Journal of Molecular Sciences
https://www.readbyqxmd.com/read/29345972/trypsin-inhibitors-from-cajanus-cajan-and-phaseolus-limensis-possess-antioxidant-anti-inflammatory-and-antibacterial-activity
#7
Tooba Naz Shamsi, Romana Parveen, Sumbul Afreen, Mudasser Azam, Priyankar Sen, Yamini Sharma, Qazi Mohd Rizwanul Haque, Tasneem Fatma, Nikhat Manzoor, Sadaf Fatima
Protease inhibitors are one of the most promising and investigated subjects for their role in pharmacognostic and pharmacological studies. This study aimed to investigate antioxidant, anti-inflammatory, and antimicrobial activities of trypsin inhibitors (TIs) from two plant sources (Cajanus cajan and Phaseolus limensis). TI was purified from C. cajan (PUSA-992) by ammonium sulfate precipitation followed by ion exchange chromatography. TI from Phaseolus limensis (lima bean trypsin inhibitor; LBTI) was procured from Sigma-Aldrich, St...
January 18, 2018: Journal of Dietary Supplements
https://www.readbyqxmd.com/read/29345928/physicochemical-properties-of-whey-protein-stabilized-astaxanthin-nanodispersion-and-its-transport-via-caco-2-monolayer
#8
Xue Shen, Changhui Zhao, Jing Lu, Mingruo Guo
Astaxanthin nanodispersion was prepared using whey protein isolate (WPI) and polymerized whey protein (PWP) through an emulsification-evaporation technique. The physicochemical properties of the astaxanthin nanodispersion were evaluated and the transport of astaxanthin was assessed using a Caco-2 cell monolayer model. The astaxanthin nanodispersions stabilized by WPI and PWP (2.5%, w/w) had a small particle size (121 ± 4.9 and 80.4 ± 5.9 nm), negative zeta potential (-19.3 ± 1.5 and -35.0 ± 2.2 mV), and high encapsulation efficiency (92...
January 18, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29345066/protease-activated-receptor-2-is-necessary-for-neutrophil-chemorepulsion-induced-by-trypsin-tryptase-or-dipeptidyl-peptidase-iv
#9
Michael J V White, Luis E Chinea, Darrell Pilling, Richard H Gomer
Compared to neutrophil chemoattractants, relatively little is known about the mechanism neutrophils use to respond to chemorepellents. We previously found that the soluble extracellular protein dipeptidyl peptidase IV (DPPIV) is a neutrophil chemorepellent. In this report, we show that an inhibitor of the protease activated receptor 2 (PAR2) blocks DPPIV-induced human neutrophil chemorepulsion, and that PAR2 agonists such as trypsin, tryptase, 2f-LIGRL, SLIGKV, and AC55541 induce human neutrophil chemorepulsion...
January 2018: Journal of Leukocyte Biology
https://www.readbyqxmd.com/read/29342974/trypsin-binding-with-copper-ions-scavenges-superoxide-molecular-dynamics-based-mechanism-investigation
#10
Xin Li, Yongliang Zhong, Chunyan Zhao
Trypsin is a serine protease, which has been proved to be a novel superoxide scavenger. The burst of superoxide induced by polychlorinated biphenyls can be impeded by trypsin in both wild type and sod knockout mutants of Escherichia coli. The experimental results demonstrated that the activities of superoxide scavenging of trypsin were significantly accelerated by Cu ions. Also, with the addition of Cu ions, a new β-sheet (β7) transited from a random coil in the Cu(II)-trypsin (TP) system, which was favorable for the formation of more contacts with other sheets of trypsin...
January 15, 2018: International Journal of Environmental Research and Public Health
https://www.readbyqxmd.com/read/29342075/enrichment-and-identification-of-the-most-abundant-zinc-binding-proteins-in-developing-barley-grains-by-zinc-imac-capture-and-nano-lc-ms-ms
#11
Giuseppe Dionisio, Mohammad Nasir Uddin, Eva Vincze
Background: Zinc accumulates in the embryo, aleurone, and subaleurone layers at different amounts in cereal grains. Our hypothesis is that zinc could be stored bound, not only to low MW metabolites/proteins, but also to high MW proteins as well. Methods: In order to identify the most abundant zinc binding proteins in different grain tissues, we microdissected barley grains into (1) seed coats; (2) aleurone/subaleurone; (3) embryo; and (4) endosperm. Initial screening for putative zinc binding proteins from the different tissue types was performed by fractionating proteins according to solubility (Osborne fractionation), and resolving those via Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) followed by polyvinylidene fluoride (PVDF) membrane blotting and dithizone staining...
January 17, 2018: Proteomes
https://www.readbyqxmd.com/read/29340436/dietary-pectin-and-mango-pulp-effects-on-small-intestinal-enzyme-activity-levels-and-macronutrient-digestion-in-grower-pigs
#12
Anton M Pluschke, Barbara A Williams, Dagong Zhang, Michael J Gidley
The effects of refined pectin and mango pulp on macronutrient digestion and small intestinal enzyme activity were studied in grower pigs. Diets based on wheat starch with and without apple pectin or dried mango fruit pulp were fed to 30 grower pigs for 21 days. Pigs were euthanized two hours postprandially, and their gastrointestinal contents recovered. Starch and protein digestion as well as α-amylase activity were all increased in pigs fed pectin. In contrast, fat digestion, lipase and protease (trypsin) activities were all significantly reduced in these pigs...
January 17, 2018: Food & Function
https://www.readbyqxmd.com/read/29339284/rbmti-6-attenuates-pathophysiological-and-inflammatory-parameters-of-induced-emphysema-in-mice
#13
Adriana Feliciano Alves Duran, Luana de Paiva Neves, Flavia Ribeiro Santos da Silva, Gabriel Capella Machado, Graziele Cristina Ferreira, Juliana D Lourenço, Aparecida Sadae Tanaka, Milton de Arruda Martins, Fernanda D T Q S Lopes, Sergio Daishi Sasaki
Protease/anti-protease imbalance is the main pathogenic mechanism of emphysema and protease inhibitors have been recognized as potential molecules to treat the disease conditions. In this work the rBmTI-6 first domain (rBmTI-6-D1), a recombinant Kunitz-type serine proteinase inhibitor, was used to verify its effect in prevention or minimization of PPE-induced emphysema in mice. C57BL/6 mice were submitted to a PPE-induced emphysema model and treated with rBmTI-6-D1 before the emphysema development. We showed that the rBmTI-6-D1 treatment was sufficient to avoid the loss of elastic recoil, an effective decrease in alveolar enlargement and in the number of macrophages and lymphocytes in bronchoalveolar lavage fluid...
January 12, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29339256/the-pbii-gene-of-the-human-salivary-proline-rich-protein-p-b-produces-another-protein-q504x8-with-an-opiorphin-homolog-qrgpr
#14
Eiichi Saitoh, Takuya Sega, Akane Imai, Satoko Isemura, Tetsuo Kato, Akihito Ochiai, Masayuki Taniguchi
OBJECTIVES: The NCBI gene database and human-transcriptome database for alternative splicing were used to determine the expression of mRNAs for P-B (SMR3B) and variant form of P-B. The translational product from the former mRNA was identified as the protein named P-B, whereas that from the latter has not yet been elucidated. In the present study, we investigated the expression of P-B and its variant form at the protein level. DESIGN: To identify the variant protein of P-B, (1) cationic proteins with a higher isoelectric point in human pooled whole saliva were purified by a two dimensional liquid chromatography; (2) the peptide fragments generated from the in-solution of all proteins digested with trypsin separated and analyzed by MALDI-TOF-MS; and (3) the presence or absence of P-B in individual saliva was examined by 15% SDS-PAGE...
January 10, 2018: Archives of Oral Biology
https://www.readbyqxmd.com/read/29337410/transcriptomic-insight-into-pathogenicity-associated-factors-of-conidiobolus-obscurus-an-obligate-aphid-pathogenic-fungus-from-entomopthoromycota
#15
Jianghong Wang, Xiang Zhou, Kai Guo, Xinqi Zhang, Haipin Lin, Cristian Montalva
BACKGROUND: Conidiobolus obscurus is a wide spread fungal entomopathogen holding aphid biocontrol potential. This study focuses on a de novo transcriptomic analysis of C. obscurus. RESULTS: A number of pathogenicity-associated factors were annotated for the first time from the assembled 17,231 fungal unigenes, including those encoding subtilisin-like proteolytic enzymes (Pr1s), trypsin-like proteases, metalloproteases, carboxypeptidases and endochitinases. Many of these genes were transcriptionally up-regulated by at least twofold in mycotized cadavers compared to the in vitro fungal cultures...
January 16, 2018: Pest Management Science
https://www.readbyqxmd.com/read/29332318/intraspecific-differences-in-plant-defense-induction-by-fall-armyworm-strains
#16
Flor E Acevedo, Michelle Peiffer, Swayamjit Ray, Robert Meagher, Dawn S Luthe, Gary W Felton
The underlying adaptive mechanisms by which insect strains are associated with specific plants are largely unknown. In this study, we investigated the role of herbivore-induced defenses in the host plant association of fall armyworm (Spodoptera frugiperda) strains. We tested the expression of herbivore-induced defense-related genes and the activity of plant-defensive proteins in maize and Bermuda grass upon feeding by fall armyworm strains. The rice strain caterpillars induced greater accumulation of proteinase inhibitors in maize than the corn strain caterpillars...
January 14, 2018: New Phytologist
https://www.readbyqxmd.com/read/29332228/comparative-evaluation-of-the-aqueous-humor-proteome-of-primary-angle-closure-and-primary-open-angle-glaucomas-and-age-related-cataract-eyes
#17
Inderjeet Kaur, Jasbir Kaur, K Sooraj, Sandeep Goswami, Rohit Saxena, Virander Singh Chauhan, Ramanjit Sihota
PURPOSE: To analyze and compare the total proteome of aqueous humor (AH) from patients having primary angle closure glaucoma (PACG), primary open angle glaucoma (POAG) and age-related cataract. MATERIALS AND METHODOLOGY: Aqueous humor was collected from age-matched PACG, POAG and cataract patients who underwent surgery, and it was immediately stored at - 80 °C until analysis. From each sample, 25 µg of total protein was subjected to trypsin digestion and subsequently LC-MS/MS analysis was performed for the deep proteome analysis...
January 13, 2018: International Ophthalmology
https://www.readbyqxmd.com/read/29332127/proteome-profiling-of-developing-murine-lens-through-mass-spectrometry
#18
Shahid Y Khan, Muhammad Ali, Firoz Kabir, Santosh Renuse, Chan Hyun Na, C Conover Talbot, Sean F Hackett, S Amer Riazuddin
Purpose: We previously completed a comprehensive profile of the mouse lens transcriptome. Here, we investigate the proteome of the mouse lens through mass spectrometry-based protein sequencing at the same embryonic and postnatal time points. Methods: We extracted mouse lenses at embryonic day 15 (E15) and 18 (E18) and postnatal day 0 (P0), 3 (P3), 6 (P6), and 9 (P9). The lenses from each time point were preserved in three distinct pools to serve as biological replicates for each developmental stage...
January 1, 2018: Investigative Ophthalmology & Visual Science
https://www.readbyqxmd.com/read/29331460/inhibition-of-shigella-sonnei-induced-epithelial-barrier-disruption-by-surface-layer-associated-proteins-of-lactobacilli-from-chinese-fermented-food
#19
Yingchun Zhang, Xiaolu Shi, Siqi Hao, Qianhui Lu, Lanwei Zhang, Xue Han, Weihong Lu
Surface-layer associated proteins (SLAP) of Lactobacillus paracasei ssp. paracasei M5-L and Lactobacillus casei Q8-L were examined to identify the functional basis for their protection within intestinal epithelial cells. The results showed that SLAP of M5-L and Q8-L remained active in a trypsin solution and retained a 45-kDa protein band, similar to that observed in controls. In contrast, under conditions of simulated gastric juice, the SLAP were partially degraded. Inhibitory effects of SLAP on adherence of Shigella sonnei to HT-29 cells were assessed with use of exclusion, competition, and replacement assays...
January 10, 2018: Journal of Dairy Science
https://www.readbyqxmd.com/read/29331348/effects-of-l-tryptophan-on-the-growth-intestinal-enzyme-activities-and-non-specific-immune-response-of-sea-cucumber-apostichopus-japonicus-selenka-exposed-to-crowding-stress
#20
Endong Zhang, Shuanglin Dong, Fang Wang, Xiangli Tian, Qinfeng Gao
In order to reveal the effects of l-tryptophan (Trp) on the physiology and immune response of sea cucumber (Apostichopus japonicus Selenka) exposed to crowding stress, four density groups of sea cucumbers (i.e. 4, 8, 16 and 32 individuals per 40 L water, represented as L, ML, MH and H) were fed with diets containing 0, 1, 3 and 5% l-tryptophan respectively for 75 days. The results showed that the specific growth rates (SGR) of the sea cucumber fed with diet with 3% Trp (L, 2.1; ML, 1.76; MH, 1.2; H, 0.7) were significantly higher than those fed with basal diet without Trp supplementation (P < ...
January 10, 2018: Fish & Shellfish Immunology
keyword
keyword
77786
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"