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https://www.readbyqxmd.com/read/29778747/novel-peptide-inhibits-inflammation-by-suppressing-of-protease-activated-receptor-2
#1
Bora Kim, Hyun-Soo Kim
We synthesized and investigated the effects of a novel peptide B, RRFSLLRY as a novel PAR-2 antagonist. Peptide B decreased calcium levels in HaCat cells stimulated with trypsin and PAR-2 activator (SLIGKV) and cytokeratin 14 and PCNA was decreased by peptide B. Peptides B also reduced the expression of inflammatory cytokines such as TNF-α and interleukin-6. Significant increase of oxazolone-induced transepidermal water loss (TEWL) was decreased by the addition of peptide B in hairless mice. These findings suggest that the anti-inflammatory effect of peptide B is due to inhibition of PAR-2 signaling...
May 17, 2018: European Journal of Pharmacology
https://www.readbyqxmd.com/read/29776812/preservation-with-%C3%AE-1-antitrypsin-improves-primary-graft-function-of-murine-lung-transplants
#2
Jessica Götzfried, Natalia F Smirnova, Carmela Morrone, Brice Korkmaz, Ali Önder Yildirim, Oliver Eickelberg, Dieter E Jenne
BACKGROUND: Vascular damage and primary graft dysfunction increase with prolonged preservation times of transplanted donor lungs. Hence, storage and conservation of donated lungs in protein-free, dextran-containing electrolyte solutions, like Perfadex, is limited to about 6 hours. We hypothesized that transplanted lungs are protected against neutrophil-mediated proteolytic damage by adding α1 -anti-trypsin (AAT), a highly abundant human plasma proteinase inhibitor, to Perfadex. METHODS: A realistic clinically oriented murine model of lung transplantation was used to simulate the ischemia-reperfusion process...
March 30, 2018: Journal of Heart and Lung Transplantation
https://www.readbyqxmd.com/read/29775708/gelatin-alginate-coacervates-for-circumventing-proteolysis-and-probing-intermolecular-interactions-by-spr
#3
Hui-Chong Lau, Seonghee Jeong, Aeri Kim
Complex coacervates based on natural biopolymers have been explored as a protein delivery system to provide controlled release of loaded protein and circumvent its proteolytic degradation in chronic wounds. The coacervates composed of gelatin A (GA) and sodium alginate (SA) were optimized with respect to turbidity, size, zeta potential, and polydispersity index. Bovine serum albumin (BSA), a model protein, was effectively encapsulated in the coacervates, resulting in protection from trypsin digestion and controlled release...
May 15, 2018: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/29775672/upregulation-of-psmb8-and-cathepsins-in-the-human-brains-of-dementia-with-lewy-bodies
#4
Qunxing Ding, Haiyan Zhu
Proteasome and lysosome are responsible for the homeostasis of proteins, lipids and carbohydrates in cells. Numerous reports indicate the proteolytic pathways have altered functions during neurodegeneration and aging. Dementia with Lewy bodies (DLB) is one of the leading forms of dementia, and the proteolytic alteration in DLB has not yet been fully investigated. This study shows that the components of proteasome and lysosome had selectively altered gene expression and enzymatic functions. Specifically, PSMB8, an inducible proteasomal β subunit, had elevated mRNA level and protein level in DLB brain compared with age-matched controls...
May 16, 2018: Neuroscience Letters
https://www.readbyqxmd.com/read/29772679/antioxidant-and-ace-inhibitory-activity-of-enzymatic-hydrolysates-from-ruditapes-philippinarum
#5
Yue Yu, Fengjiao Fan, Di Wu, Cuiping Yu, Zhenyu Wang, Ming Du
Ruditapes philippinarum proteins were hydrolyzed by trypsin, neutrase, and pepsin. The antioxidant activities and ACE inhibitory activity of hydrolysates were analyzed and the antioxidant activities were related to their molecular weight distribution and amino acid compositions. Results indicated the hydrolysis of proteins led to an increase in small peptides and free amino acids. The antioxidant activities of Ruditapes philippinarum hydrolysates against DPPH radical scavenging, inhibition on linoleic acid peroxidation, and reducing power showed that the neutrase hydrolysate exhibited the strongest antioxidant activity...
May 16, 2018: Molecules: a Journal of Synthetic Chemistry and Natural Product Chemistry
https://www.readbyqxmd.com/read/29771485/dual-sensitive-hydrogel-nanoparticles-based-on-conjugated-thermoresponsive-copolymers-and-protein-filaments-for-triggerable-drug-delivery
#6
Roujin Ghaffari, Niloofar Eslahi, Elnaz Tamjid, Abdolreza Arash Simchi
Novel hydrogel nanoparticles with dual triggerable release properties based on fibrous structural proteins (Keratin) and thermoresponsive copolymers (Pluronic) is introduced. The nanoparticle was used for Curcumin delivery, as an effective and safe anticancer agent, hydrophobicity of which has limited its clinical application. The drug was loaded into hydrogel nanoparticles by a single-step nanoprecipitation method. The drug-loaded nanoparticles had an average dimeter of 165 nm and 66 nm at 25ºC and 37ºC, respectively...
May 17, 2018: ACS Applied Materials & Interfaces
https://www.readbyqxmd.com/read/29770328/second-blood-meal-by-female-lutzomyia-longipalpis-enhancement-by-oviposition-and-its-effects-on-digestion-longevity-and-leishmania-infection
#7
C S Moraes, K Aguiar-Martins, S G Costa, P A Bates, R J Dillon, F A Genta
Lutzomyia longipalpis is the main vector of visceral leishmaniasis (VL) in America. Physiological and molecular mechanisms of Leishmania infection in sand flies have been studied during the first gonotrophic cycle. There are few studies about these interactions during the second gonotrophic cycle mainly because of the difficulties maintaining sand flies through sequential feeds. Here we standardized conditions to perform the second blood feed efficiently, and our results show that oviposition is an essential factor for the success of multiple feeds...
2018: BioMed Research International
https://www.readbyqxmd.com/read/29770172/prevention-of-cross-contamination-risk-by-disinfection-of-irreversible-hydrocolloid-impression-materials-with-ozonated-water
#8
Omid Savabi, Farahnaz Nejatidanesh, Kamran Poshang Bagheri, Leila Karimi, Ghazal Savabi
Background: Disinfection of dental impression is mandatory for preventing the cross infection between dental staff and dental laboratory. Ozone as a disinfectant can be used in various ways in dentistry. The aim of this study was to evaluate the efficacy of ozonated water for disinfection of irreversible hydrocolloid impression materials and preventing cross-contamination. Methods: Thirty-two circular specimens (1 cm diameter and 2 mm thickness) of irreversible hydrocolloid impression material were made...
2018: International Journal of Preventive Medicine
https://www.readbyqxmd.com/read/29768046/does-partial-titin-degradation-affect-sarcomere-length-non-uniformities-and-force-in-active-and-passive-myofibrils
#9
Venus Joumaa, Fanny Bertrand, Shuyue Liu, Sophia Poscente, Walter Herzog
The aim of this study was to determine the role of titin in preventing the development of sarcomere length non-uniformities following activation and after active and passive stretch, by determining the effect of partial titin degradation on sarcomere length non-uniformities and force in passive and active myofibrils. Selective partial titin degradation was performed using a low dose of trypsin. Myofibrils were set at a sarcomere length of 2.4 µm and then passively stretched to sarcomere lengths of 3.4 µm and 4...
May 16, 2018: American Journal of Physiology. Cell Physiology
https://www.readbyqxmd.com/read/29766714/the-outer-membrane-vesicle-proteome-of-porphyromonas-gingivalis-is-differentially-modulated-relative-to-the-outer-membrane-in-response-to-heme-availability
#10
Paul D Veith, Caroline Luong, Keng H Tan, Stuart G Dashper, Eric C Reynolds
Porphyromonas gingivalis is an anaerobic, Gram-negative oral pathogen associated with chronic periodontitis. P. gingivalis has an obligate requirement for heme which it obtains from the host. Heme availability has been linked to disease initiation and progression. In this study we used continuous culture of the bacterium to determine the effect of heme limitation and excess on the P. gingivalis proteome. Four biological replicates of whole cell lysate (WCL) and outer membrane vesicle (OMV) samples were digested with trypsin and analysed by tandem mass spectrometry and MaxQuant label-free quantification...
May 16, 2018: Journal of Proteome Research
https://www.readbyqxmd.com/read/29765540/could-a-plant-derived-protein-potentiate-the-anticancer-effects-of-a-stem-cell-in-brain-cancer
#11
Camila Ramalho Bonturi, Helena Motaln, Mariana Cristina Cabral Silva, Bruno Ramos Salu, Marlon Vilela de Brito, Luciana de Andrade Luz Cost, Heron Fernandes Vieira Torquato, Natalia Neto Dos Santos Nunes, Edgar Julian Paredes-Gamero, Tamara Lah Turnšek, Maria Luiza Vilela Oliva
Glioblastoma is the most aggressive brain tumor with poor overall survival bellow 2 years. The natural compounds with anti-cancer properties, are thus gaining attention for possible adjuvant GBM treatment. In various cancer models Enterolobium contortisiliquum Trypsin Inhibitor (EcTI) proved to have anti-cancer effects. Here, we investigated the EcTI effects on GBM U87 cells and on mesenchymal stem cells (MSC) compared to their direct coculture (MSC/U87). MSC are present in tumor stroma, modulating GBM cells phenotype, and also represent potential drug delivery vehicle due to their tumor tropism...
April 20, 2018: Oncotarget
https://www.readbyqxmd.com/read/29761912/proteinase-pattern-of-honeybee-prepupae-from-healthy-and-american-foulbrood-infected-bees-investigated-by-zymography-the-numbered-spots-figure-4-were-excised-from-the-gels-and-they-were-individually-transferred-to-a-1-5-ml-microcentrifuge-tube-spots-were-destained
#12
Proteinase pattern of honeybee prepupae from healthy and American Foulbrood infected bees investigated by zymography: The numbered spots (figure 4) were excised from the gels and they were individually transferred to a 1.5 mL microcentrifuge tube. Spots were destained and proteins digested as reported by Dani et al. 40. Each peptide mixture was submitted to capillary-LC-μESI-MS/MS analysis on an Ultimate 3000 HPLC (Dionex, San Donato Milanese, Milano, Italy) coupled to a LTQ Orbitrap mass spectrometer (Thermo Fisher, Bremen, Germany). Peptides were concentrated on a precolumn cartridge PepMap100 C18 (300 μm i.d. × 5 mm, 5 μm, 100 Å, LC Packings Dionex) and then eluted on a homemade capillary column packed with Aeris Peptide XB-C18 phase (180 μm i.d. × 15 cm, 3.6 μm, 100 Å, Phenomenex, Torrance, CA, USA) at 1 μl/min. The loading mobile phases were: 0.1% TFA in H 2 O (phase A) and 0.1% TFA in CH 3 CN (phase B). The elution mobile phases composition was: H 2 O 0.1% formic acid/CH 3 CN 97/3 (phase A) and CH 3 CN 0.1% formic acid/ H 2 O 97/3 (phase B). The elution program was: 0 min, 4% B; 10 min, 40% B; 30 min, 65% B; 35 min, 65% B; 36 min, 90% B; 40 min, 90% B; 41 min, 4%B; 60 min, 4% B. Mass spectra were acquired in positive ion mode, setting the spray voltage at 1.8 kV, the capillary voltage and temperature respectively at 45 V and 200°C, and the tube lens at 130 V. Data were acquired in data-dependent mode with dynamic exclusion enabled (repeat count 2, repeat duration 15 s, exclusion duration 30 s); survey MS scans were recorded in the Orbitrap analyzer in the mass range 300-2000 m/z at a 15,000 nominal resolution at m/z = 400; then up to three most intense ions in each full MS scan were fragmented (isolation width 3 m/z, normalized collision energy 30) and analyzed in the IT analyzer. Monocharged ions did not trigger MS/MS experiments.
Antonio Felicioli, Barbara Turchi, Filippo Fratini, Matteo Giusti, Roberta Nuvoloni, Francesca Romana Dani, Simona Sagona
American foulbrood disease (AFB) is the main devastating disease that affects honeybees' brood, caused by Paenibacillus larvae. The trend of the research on AFB has addressed the mechanisms by which P. larvae bacteria kill honeybee larvae. Since prepupae could react to the infection of AFB by increasing protease synthesis, the aim of this work was to compare protease activity in worker prepupae belonging to healthy colonies and to colonies affected by AFB. This investigation was performed by zymography. In gel, proteolytic activity was observed in prepupae extracts belonging to the healthy colonies...
May 15, 2018: Electrophoresis
https://www.readbyqxmd.com/read/29761495/changes-in-gene-expression-during-germination-reveal-pea-genotypes-with-either-quiescence-or-escape-mechanisms-of-waterlogging-tolerance
#13
Md Shahin Uz Zaman, Al Imran Malik, William Erskine, Parwinder Kaur
Waterlogging causes germination failure in pea (Pisum sativum L.). Three genotypes (BM-3, NL-2 and Kaspa) contrasting in ability to germinate in waterlogged soil were exposed to different durations of waterlogging. Whole genome RNAseq was employed to capture differentially expressing genes. The ability to germinate in waterlogged soil was associated with testa colour and testa membrane integrity as confirmed by electrical conductivity measurements. Genotypes Kaspa and NL-2 displayed different mechanisms of tolerance...
May 14, 2018: Plant, Cell & Environment
https://www.readbyqxmd.com/read/29760205/abnormal-plasma-clot-formation-and-fibrinolysis-reveal-bleeding-tendency-in-patients-with-partial-factor-xi-deficiency
#14
Gillian N Gidley, Lori A Holle, John Burthem, Paula H B Bolton-Maggs, Feng-Chang Lin, Alisa S Wolberg
Individuals with factor XI (FXI) deficiency have a variable bleeding risk that cannot be predicted from plasma FXI antigen or activity. This limitation can result in under- or overtreatment of patients and risk of bleeding or thrombosis. Previously, plasma clot fibrinolysis assays showed sensitivity to bleeding tendency in a small cohort of patients with severe FXI deficiency. Here, we determined the ability of plasma clot formation, structure, and fibrinolysis assays to predict bleeding tendency in a larger, independent cohort of patients with severe and partial FXI deficiency...
May 22, 2018: Blood Advances
https://www.readbyqxmd.com/read/29758537/biopolymers-conjugated-with-magnetite-as-support-materials-for-trypsin-immobilization-and-protein-digestion
#15
Jakub Zdarta, Katarzyna Antecka, Artur Jędrzak, Karol Synoradzki, Magdalena Łuczak, Teofil Jesionowski
In the presented study synthesized magnetic nanoparticles were used as an inorganic precursor for the preparation of novel magnetite-lignin and magnetite-chitin hybrid supports for enzyme immobilization. Effective synthesis of the hybrids was confirmed by Fourier transform infrared spectroscopy and powder X-ray diffraction analysis. The materials exhibited good thermal stability and surface areas of 4.3 and 5.6 m2 /g respectively. The magnetite-lignin + trypsin and magnetite-chitin + trypsin systems were found to have good storage stability and reusability...
May 8, 2018: Colloids and Surfaces. B, Biointerfaces
https://www.readbyqxmd.com/read/29758346/electro-responsive-graphene-oxide-hydrogels-for-skin-bandages-the-outcome-of-gelatin-and-trypsin-immobilization
#16
Mariagrazia di Luca, Orazio Vittorio, Giuseppe Cirillo, Manuela Curcio, Magdalena Czuban, Florida Voli, Annafranca Farfalla, Silke Hampel, Fiore Pasquale Nicoletta, Francesca Iemma
A free radical polymerization method was adopted for the fabrication of hybrid hydrogel films based on acrylamide and polyethylene glycol dimethacrylate as plasticizing and crosslinking agents, respectively, to be employed as smart skin bandages. Electro-sensitivity, biocompatibility and proteolytic properties were conferred to the final polymer networks by introducing graphene oxide (0.5% w/w), gelatin or trypsin (10% w/w) in the polymerization feed. The physical chemical and mechanical characterization of hybrid materials was performed by means of determination of protein content, Raman spectroscopy, thermogravimetric analysis and measurement of tensile strength...
May 11, 2018: International Journal of Pharmaceutics
https://www.readbyqxmd.com/read/29758270/collagen-gly-missense-mutations-effect-of-residue-identity-on-collagen-structure-and-integrin-binding
#17
Yimin Qiu, Arya Mekkat, Hongtao Yu, Sezin Yigit, Samir Hamaia, Richard W Farndale, David L Kaplan, Yu-Shan Lin, Barbara Brodsky
Gly missense mutations in type I collagen, which replace a conserved Gly in the repeating (Gly-Xaa-Yaa)n sequence with a larger residue, are known to cause Osteogenesis Imperfecta (OI). The clinical consequences of such mutations range from mild to lethal, with more serious clinical severity associated with larger Gly replacement residues. Here, we investigate the influence of the identity of the residue replacing Gly within and adjacent to the integrin binding 502 GFPGER507 sequence on triple-helix structure, stability and integrin binding using a recombinant bacterial collagen system...
May 11, 2018: Journal of Structural Biology
https://www.readbyqxmd.com/read/29756774/selection-identification-and-binding-mechanism-studies-of-an-ssdna-aptamer-targeted-to-different-stages-of-e-coli-o157-h7
#18
Ying Zou, Nuo Duan, Shijia Wu, Mofei Shen, Zhouping Wang
Enterohemorrhagic Escherichia coli O157:H7 (E.coli O157:H7) is known as an important food-borne pathogens related to public health. In this study, aptamers which could bind to different stages of E.coli O157:H7 (adjustment phase, log phase and stationary phase) with high affinity and specificity was obtained by Whole Cell-SELEX method through fourteen selection rounds including three counter-selection rounds. Altogether, thirty-two sequences were obtained and nine families were classified to select the optimal aptamer...
May 14, 2018: Journal of Agricultural and Food Chemistry
https://www.readbyqxmd.com/read/29754319/responses-of-rainbow-trout-intestinal-epithelial-cells-to-different-kinds-of-nutritional-deprivation
#19
Patrick G Pumputis, Vivian R Dayeh, Lucy E J Lee, Phuc H Pham, Zhenzhen Liu, Senthuri Viththiyapaskaran, Niels C Bols
In order to develop an in vitro system to study the cell biology of starvation in the fish intestine, rainbow trout intestinal epithelial cells were subjected to three kinds of nutrient deprivation and evaluated for 7 days. The RTgutGC cell line was grown into monolayers in Leibovitz's basal medium supplemented with fetal bovine serum (L15/FBS) and then subjected to deprivation of serum (L15); of serum, amino acids, and vitamin (L15/ex); and of all nutrients (L15/salts). After 7 days of nutrient deprivation, the cells remained attached to the plastic surface as monolayers but changes were seen in shape, with the cells becoming more polygonal, actin and α-tubulin cytoskeleton organization, and in tight junction protein-1 (ZO-1) localization...
May 12, 2018: Fish Physiology and Biochemistry
https://www.readbyqxmd.com/read/29753984/a-novel-white-spot-syndrome-virus-protein-wssv164-controls-prophenoloxidases-pmpropos-in-shrimp-melanization-cascade
#20
Pakkakul Sangsuriya, Walaiporn Charoensapsri, Jantiwan Sutthangkul, Saengchan Senapin, Ikuo Hirono, Anchalee Tassanakajon, Piti Amparyup
Melanization, mediated by the prophenoloxidase (proPO)-activating system, is an important innate immune response in invertebrates. The implication of the proPO system in antiviral response and the suppression of host proPO activation by the viral protein have previously been demonstrated in shrimp. However, the molecular mechanism of viral-host interactions in the proPO cascade remains largely unexplored. Here, we characterized the viral protein, namely, WSSV164, which was initially identified from the forward suppression subtractive hybridization (SSH) cDNA library of the PmproPO1/2 co-silenced black tiger shrimp Penaeus monodon that was challenged with white spot syndrome virus (WSSV)...
May 4, 2018: Developmental and Comparative Immunology
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