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https://www.readbyqxmd.com/read/28544808/the-first-transepidermal-transplantation-of-non-cultured-epidermal-suspension-using-a-dermarolling-system-in-vitiligo-a-sequential-histological-and-clinical-study
#1
L Benzekri, Y Gauthier
The current methods for melanocyte delivery to depigmented skin are invasive and often require sophisticated approaches. Here we describe a promising simple and minimally invasive technique based on the dermarolling system. The technique involves preparation of a keratinocyte/melanocyte suspension prepared by trypsinization from a non-lesioned part of a patient's scalp skin and transepidermal delivery using a dermaroller equipped with 0.2 mm needles. Dermarolling leads to epidermal microinjuries without, however, causing pain or inflammation...
May 19, 2017: Pigment Cell & Melanoma Research
https://www.readbyqxmd.com/read/28542501/a-rho-associated-coiled-coil-containing-kinases-rock-inhibitor-y-27632-enhances-adhesion-viability-and-differentiation-of-human-term-placenta-derived-trophoblasts-in-vitro
#2
Kenichiro Motomura, Naoko Okada, Hideaki Morita, Mariko Hara, Masato Tamari, Keisuke Orimo, Go Matsuda, Ken-Ichi Imadome, Akio Matsuda, Takeshi Nagamatsu, Mikiya Fujieda, Haruhiko Sago, Hirohisa Saito, Kenji Matsumoto
Although human term placenta-derived primary cytotrophoblasts (pCTBs) represent a good human syncytiotrophoblast (STB) model, in vitro culture of pCTBs is not always easily accomplished. Y-27632, a specific inhibitor of Rho-associated coiled-coil containing kinases (ROCK), reportedly prevented apoptosis and improved cell-to-substrate adhesion and culture stability of dissociated cultured human embryonic stem cells and human corneal endothelial cells. The Rho kinase pathway regulates various kinds of cell behavior, some of which are involved in pCTB adhesion and differentiation...
2017: PloS One
https://www.readbyqxmd.com/read/28542244/a-quantitative-label-free-analysis-of-the-extracellular-proteome-of-human-supraspinatus-tendon-reveals-damage-to-the-pericellular-and-elastic-fibre-niches-in-torn-and-aged-tissue
#3
Osnat Hakimi, Nicola Ternette, Richard Murphy, Benedikt M Kessler, Andrew Carr
Tears of the human supraspinatus tendon are common and often cause painful and debilitating loss of function. Progressive failure of the tendon leading to structural abnormality and tearing is accompanied by numerous cellular and extra-cellular matrix (ECM) changes in the tendon tissue. This proteomics study aimed to compare torn and aged rotator cuff tissue to young and healthy tissue, and provide the first ECM inventory of human supraspinatus tendon generated using label-free quantitative LC-MS/MS. Employing two digestion protocols (trypsin and elastase), we analysed grain-sized tendon supraspinatus biopsies from older patients with torn tendons and from healthy, young controls...
2017: PloS One
https://www.readbyqxmd.com/read/28541760/inhibition-of-proteases-activity-in-intestine-needs-a-sustainable-acidic-environment-rather-than-a-transient
#4
Chang Xing, Jin-Feng Xing, Zhi-Qiang Ge
α-Chymotrypsin (α-CT) and trypsin are important components of the enzymatic barrier. They could degrade the therapeutic proteins and peptides, inhibit their activity consequently, and thereby reduce their oral bioavailability. Acidic agents, as one type of indirect protease inhibitors, have shown proof of concept in clinical trials. We report here the inactivated proteases due to acid influence can be reactivated immediately by environmental pH recovery regardless of how long the inactivation last. To keep the inactivation time of proteases for 4 to 5 h, we designed and prepared a sustained-release tablet containing citric acid (CA) which can effectively reduce the pH below 5...
May 25, 2017: Drug Development and Industrial Pharmacy
https://www.readbyqxmd.com/read/28541676/double-sweeping-highly-effective-sample-preconcentration-using-cationic-and-anionic-micelles-and-its-application-to-a-multiple-enzyme-activity-assay
#5
Ryota Sanuki, Kenji Sueyoshi, Tatsuro Endo, Hideaki Hisamoto
In conventional microfluidic bioassays, short optical pathlength and extremely small quantities of analytes sometimes can significantly reduce detectability. Various sample preconcentration techniques have been reported for improving the detectability of bioassays. In the present study, we developed a novel preconcentration technique, 'double sweeping', utilizing cationic and anionic micelles simultaneously. Microscopic observations demonstrated that double sweeping enabled a sample solution, which initially filled a capillary, to be focused into an extremely narrow band...
May 25, 2017: Analytical Chemistry
https://www.readbyqxmd.com/read/28540009/molecular-imaging-of-retinal-endothelial-injury-in-diabetic-animals
#6
Sonja Frimmel, Souska Zandi, Dawei Sun, Zhongyu Zhang, Alexander Schering, Mark I Melhorn, Shintaro Nakao, Ali Hafezi-Moghadam
PURPOSE: Diabetic retinopathy is a leading cause of vision loss. There is a great need for early diagnosis prior to the occurrence of irreversible structural damages. Expression of endothelial adhesion molecules is observed before the onset of diabetic vascular damage; however, to date, these molecules cannot be visualized in vivo. METHODS: To quantify the expression of endothelial surface molecules, we generated imaging probes that bind to ICAM-1. The α-ICAM-1 probes were characterized via flow cytometry under microfluidic conditions...
April 2017: Journal of Ophthalmic & Vision Research
https://www.readbyqxmd.com/read/28537661/effect-of-urinary-trypsin-inhibitor-on-inflammatory-cytokines-and-organ-function-in-patients-with-cardiopulmonary-bypass
#7
H-Y Xu, X-S Rong, D-P Wang, S-Y Jiang, Z-D Zang, W Xia, F Zhang, J Yan
OBJECTIVE: This research is to study the effect of urinary trypsin inhibitor (UTI) on inflammatory cytokines and organ function in patients with cardiopulmonary bypass. PATIENTS AND METHODS: From February 2015 to February 2016, 40 patients that had undergone cardiopulmonary bypass surgery in our hospital were selected and randomly divided into the observation group and the control group with 20 patients in each group. Patients in the control group were intravenously injected with 5000 U/kg normal saline during the operation and 5000 U/kg•d-1 at 1-3 days postoperatively, while the patients in the observation group received intravenous injection of the same amount of UTI at pre-operation (T0), post-anesthesia (T1), after aortic opening (T2), after cardiopulmonary bypass 4h (T3), 8h (T4), 24h (T5), 48h (T6), and 72h (T7)...
May 2017: European Review for Medical and Pharmacological Sciences
https://www.readbyqxmd.com/read/28537546/biological-characterisation-of-the-emerged-highly-pathogenic-avian-influenza-hpai-a-h7n9-viruses-in-humans-in-mainland-china-2016-to-2017
#8
Wenfei Zhu, Jianfang Zhou, Zi Li, Lei Yang, Xiyan Li, Weijuan Huang, Sumei Zou, Wenbing Chen, Hejiang Wei, Jing Tang, Liqi Liu, Jie Dong, Dayan Wang, Yuelong Shu
With no or low virulence in poultry, avian influenza A(H7N9) virus has caused severe infections in humans. In the current fifth epidemic wave, a highly pathogenic avian influenza (HPAI) H7N9 virus emerged. The insertion of four amino acids (KRTA) at the haemagglutinin (HA) cleavage site enabled trypsin-independent infectivity of this virus. Although maintaining dual receptor-binding preference, its HA antigenicity was distinct from low-pathogenic avian influenza A(H7N9). The neuraminidase substitution R292K conferred a multidrug resistance phenotype...
May 11, 2017: Euro Surveillance: Bulletin Européen sur les Maladies Transmissibles, European Communicable Disease Bulletin
https://www.readbyqxmd.com/read/28537193/isolated-potato-virus-a-coat-protein-possesses-unusual-properties-and-forms-different-short-virus-like-particles
#9
Alexander L Ksenofontov, Eugeny N Dobrov, Natalia V Fedorova, Marina V Serebryakova, Andrei N Prusov, Ludmila A Baratova, Viiu Paalme, Lilian Järvekülg, Eleonora V Shtykova
In our previous study, we have observed that the isolated coat proteins (CP) of the Potyvirus Potato Virus A (PVA) virions exhibit an intrinsic tendency of to self-associate into various multimeric forms containing some fractions of cross-β-structure. In this report, we studied the effect of solution conditions on the structure and dissociation of isolated PVA CP using a number of complementary physicochemical methods. Analysis of the structure of PVA CP in solution was performed by limited proteolysis with MALDI-TOF Mass Spectrometry analysis, transmission electron microscopy, intrinsic fluorescence spectroscopy and synchrotron small angle X-ray scattering (SAXS)...
May 24, 2017: Journal of Biomolecular Structure & Dynamics
https://www.readbyqxmd.com/read/28536777/genetic-risk-in-chronic-pancreatitis-the-trypsin-dependent-pathway
#10
REVIEW
Eszter Hegyi, Miklós Sahin-Tóth
Genetic investigations have provided unique insight into the mechanism of chronic pancreatitis in humans and firmly established that uncontrolled trypsin activity is a central pathogenic factor. Mutations in the PRSS1, SPINK1, and CTRC genes promote increased activation of trypsinogen to trypsin by stimulation of autoactivation or by impairing protective trypsinogen degradation and/or trypsin inhibition. Here we review key genetic and biochemical features of the trypsin-dependent pathological pathway in chronic pancreatitis...
May 23, 2017: Digestive Diseases and Sciences
https://www.readbyqxmd.com/read/28536266/two-proteins-for-the-price-of-one-structural-studies-of-the-dual-destiny-preproalbumin-with-sunflower-trypsin-inhibitor-1
#11
Bastian Franke, Amy M James, Mehdi Mobli, Michelle L Colgrave, Joshua S Mylne, K Johan Rosengren
Seed storage proteins are both an important source of nutrition for humans and essential for seedling establishment. Interestingly, unusual napin-type 2S seed storage albumin precursors in sunflower contain a sequence that is released as a macrocyclic peptide during post-translational processing. The mechanism by which such peptides emerge from linear precursors proteins has received increased attention, however the structural characterization of intact precursor proteins has been limited. Here we report the 3D NMR structure of the Helianthus annuus PreproAlbumin With Sunflower trypsin inhibitor-1 (PawS1), and provide new insights into the processing of this remarkable dual-destiny protein...
May 23, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28535747/in-vitro-assessment-of-pediococcus-acidilactici-kp10-for-its-potential-use-in-the-food-industry
#12
Sahar Abbasiliasi, Joo Shun Tan, Fatemeh Bashokouh, Tengku Azmi Tengku Ibrahim, Shuhaimi Mustafa, Faezeh Vakhshiteh, Subhashini Sivasamboo, Arbakariya B Ariff
BACKGROUND: Selection of a microbial strain for the incorporation into food products requires in vitro and in vivo evaluations. A bacteriocin-producing lactic acid bacterium (LAB), Pediococcus acidilactici Kp10, isolated from a traditional dried curd was assessed in vitro for its beneficial properties as a potential probiotic and starter culture. The inhibitory spectra of the bacterial strain against different gram-positive and gram-negative bacteria, its cell surface hydrophobicity and resistance to phenol, its haemolytic, amylolytic and proteolytic activities, ability to produce acid and coagulate milk together with its enzymatic characteristics and adhesion property were all evaluated in vitro...
May 23, 2017: BMC Microbiology
https://www.readbyqxmd.com/read/28533236/antibacterial-and-antibiofilm-activities-of-a-novel-synthetic-cyclic-lipopeptide-against-cariogenic-streptococcus-mutans-ua159
#13
Kyung R Min, Adriana Galvis, Brandon Williams, Ramanjaneyulu Rayala, Predrag Cudic, Dragana Ajdic
Despite continuous efforts to control cariogenic dental biofilms, very few effective antimicrobial treatments exist. In this study, we characterized the activity of a novel synthetic cyclic lipopeptide 4 (CLP-4), derived from fusaricidin, against the cariogenic pathogen Streptococcus mutans UA159. We determined CLP-4's minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), spontaneous resistance frequency, and we performed time-kill assays. Additionally, we assessed CLP-4's potential to inhibit biofilm formation (MBIC) and eradicate pre-formed biofilms (MBEC)...
May 22, 2017: Antimicrobial Agents and Chemotherapy
https://www.readbyqxmd.com/read/28529592/unique-insight-into-microenvironmental-changes-in-colorectal-cancer-ex-vivo-assessment-of-matrix-metalloprotease-mediated-molecular-changes-in-human-colorectal-tumor-tissue-and-corresponding-non-neoplastic-adjacent-tissue
#14
Nicholas Willumsen, Cecilie L Bager, Anne-Christine Bay-Jensen, Stephanie N Kehlet, Henrik Harling, Diana J Leeming, Morten A Karsdal, Lars N Jorgensen
Matrix metalloprotease (MMP)-mediated tissue remodeling is one of the malignant changes driving colorectal cancer. Measurement of altered MMP expression/activity is not sufficient to fully understand the effect of MMP-mediated tissue remodeling. Biomarkers are required that specifically reflect the dynamic processes of the MMP-mediated degradation of signature proteins from colorectal tissue. The aim of the present study was to profile and characterize the release of MMP-degraded type III collagen (C3M) and citrullinated and MMP-degraded vimentin (VICM) from tumor tissue and corresponding non-neoplastic adjacent tissue (NAT) in a human colorectal cancer ex vivo model...
May 2017: Oncology Letters
https://www.readbyqxmd.com/read/28529409/an-experimental-check-of-backscattering-interferometry
#15
Michael M Baksh, M G Finn
Backscattering interferometry (BSI) was used to determine the association constants for four well-known biomolecular interactions: protein A + IgG, trypsin + antitrypsin, trypsin + p-aminobenzamidine, and antithrombin + heparin. Each gave well-defined binding curves and Kd values in close agreement with published findings obtained using other techniques. These results stand in direct contrast to the claims in a 2015 publication in this journal (Discussion of "Back Scattering Interferometry revisited-a theoretical and experimental investigation" Jørgensen, T...
May 2017: Sensors and Actuators. B, Chemical
https://www.readbyqxmd.com/read/28528957/proteasomes-in-corneal-epithelial-cells-and-cultured-autologous-oral-mucosal-epithelial-cell-sheet-caomecs-graft-used-for-the-ocular-surface-regeneration
#16
Fawzia Bardag-Gorce, Richard Hoft, Imara Meepe, Julio Garcia, Kumar Tiger, Andrew Wood, Amanda Laporte, Derek Pan, Andrew Makalinao, Robert Niihara, Joan Oliva, Arjie Florentino, Amber M Gorce, Jeremy Stark, Daileen Cortez, Samuel W French, Yutaka Niihara
PURPOSE: This study focuses on characterizing proteasomes in corneal epithelial cells (CEC) and in cultured autologous oral mucosal epithelial cell sheets (CAOMECS) used to regenerate the ocular surface. METHODS: Limbal stem cell deficiency (LSCD) was surgically induced in rabbit corneas. CAOMECS was engineered and grafted onto corneas with LSCD to regenerate the ocular surface. RESULTS: LSCD caused an increase in inflammatory cells in the ocular surface, an increase in the formation of immunoproteasomes (IPR), and a decrease in the formation of constitutive proteasome (CPR)...
May 18, 2017: Ocular Surface
https://www.readbyqxmd.com/read/28528000/biophysical-insight-into-structure-function-relation-of-allium-sativum-protease-inhibitor-by-thermal-chemical-and-ph-induced-modulation-using-comprehensive-spectroscopic-analysis
#17
Tooba Naz Shamsi, Romana Parveen, Huma Naz, Md Anzarul Haque, Sadaf Fatima
In this study, we have analyzed the structural and functional changes in the nature of Allium sativum Protease Inhibitor (ASPI) on undergoing various denaturation with variable range of pH, temperature and urea (at pH 8.2). ASPI being anti-tryptic in nature has native molecular mass of ∼15kDa. The conformational stability, functional parameters and their correlation were estimated under different conditions using circular dichroism, fluorescence and activity measurements. ASPI was found to fall in belongs to α+β protein...
May 17, 2017: International Journal of Biological Macromolecules
https://www.readbyqxmd.com/read/28526811/proteomic-analysis-of-cerebrospinal-fluid-from-children-with-central-nervous-system-tumors-identifies-candidate-proteins-relating-to-tumor-metastatic-spread
#18
Filippo Spreafico, Italia Bongarzone, Sara Pizzamiglio, Ruben Magni, Elena Taverna, Maida De Bortoli, Chiara M Ciniselli, Elena Barzanò, Veronica Biassoni, Alessandra Luchini, Lance A Liotta, Weidong Zhou, Michele Signore, Paolo Verderio, Maura Massimino
Central nervous system (CNS) tumors are the most common solid tumors in childhood. Since the sensitivity of combined cerebrospinal fluid (CSF) cytology and radiological neuroimaging in detecting meningeal metastases remains relatively low, we sought to characterize the CSF proteome of patients with CSF tumors to identify biomarkers predictive of metastatic spread. CSF samples from 27 children with brain tumors and 13 controls (extra-CNS non-Hodgkin lymphoma) were processed using core-shell hydrogel nanoparticles, and analyzed with reverse-phase liquid chromatography/electrospray tandem mass spectrometry (LC-MS/MS)...
May 3, 2017: Oncotarget
https://www.readbyqxmd.com/read/28525322/a-systemic-review-of-existing-serological-possibilities-to-diagnose-canine-osteoarthritis-with-a-particular-focus-on-extracellular-matrix-proteoglycans-and-protein
#19
M Shahid, G Manchi, P Slunsky, O Naseer, A Fatima, B Leo, J Raila
Extra-cellular matrix (ECM) components are important and their stabilization is significant in maintaining normal healthy joint environment. In osteoarthritis (OA), ECM components are altered and indicate disease progression. The joint ECM is composed of proteoglycans (aggrecan, perlecan, inter α-trypsin inhibitor), glycoproteins (fibronectin, lubricin, COMP) and collagen types (most abundantly collagen type II) which represent structural and functional transformation during disease advancement. ECM investigation revealed significant biomarkers of OA that could be used as a diagnostic and therapeutic tool in different canine orthopedic diseases...
March 28, 2017: Polish Journal of Veterinary Sciences
https://www.readbyqxmd.com/read/28524070/tmprss2-and-mspl-facilitate-trypsin-independent-porcine-epidemic-diarrhea-virus-replication-in-vero-cells
#20
Wen Shi, Wenlu Fan, Jing Bai, Yandong Tang, Li Wang, Yanping Jiang, Lijie Tang, Min Liu, Wen Cui, Yigang Xu, Yijing Li
Type II transmembrane serine proteases (TTSPs) facilitate the spread and replication of viruses such as influenza and human coronaviruses, although it remains unclear whether TTSPs play a role in the progression of animal coronavirus infections, such as that by porcine epidemic diarrhea virus (PEDV). In this study, TTSPs including TMPRSS2, HAT, DESC1, and MSPL were tested for their ability to facilitate PEDV replication in Vero cells. Our results showed that TMPRSS2 and MSPL played significant roles in the stages of cell-cell fusion and virus-cell fusion, whereas HAT and DESC1 exhibited weaker effects...
May 18, 2017: Viruses
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