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https://www.readbyqxmd.com/read/28444931/study-of-structure-dependent-chromatographic-behavior-of-glycopeptides-using-reversed-phase-nanolc
#1
Petr Kozlik, Radoslav Goldman, Miloslav Sanda
Analysis of glycosylation is challenging due to micro- and macro-heterogeneity of the protein attachment. A combination of liquid chromatography with tandem mass spectrometry is one of the most powerful tools for glycopeptide analysis. In this work, we show the effect of various monosaccharide units on the retention time of glycopeptides. Retention behavior of several glycoforms of six peptides obtained from tryptic digest of haptoglobin, hemopexin and sex hormone binding globulin was studied on a reversed phase chromatographic column...
April 26, 2017: Electrophoresis
https://www.readbyqxmd.com/read/28444616/genome-wide-identification-of-grain-filling-genes-regulated-by-the-ossmf1-transcription-factor-in-rice
#2
Joung Sug Kim, Songhwa Chae, Kyong Mi Jun, Yoon-Mok Pahk, Tae-Ho Lee, Pil Joong Chung, Yeon-Ki Kim, Baek Hie Nahm
BACKGROUND: Spatial- and temporal-specific expression patterns are primarily regulated at the transcriptional level by gene promoters. Therefore, it is important to identify the binding motifs of transcription factors to better understand the networks associated with embryogenesis. RESULTS: Here, we used a protein-binding microarray (PBM) to identify the binding motifs of OsSMF1, which is a basic leucine zipper transcription factor involved in the regulation of rice seed maturation...
December 2017: Rice
https://www.readbyqxmd.com/read/28442108/effect-of-egyptian-propolis-on-cryptosporidiosis-in-immunosuppressed-rats-with-special-emphasis-on-oocysts-shedding-leukogram-protein-profile-and-ileum-histopathology
#3
Hamdy Soufy, Nadia M El-Beih, Soad M Nasr, Tamer H Abd El-Aziz, Fathia A M Khalil, Youssef F Ahmed, Hala A A Abou Zeina
OBJECTIVES: To investigate the activity of Egyptian propolis extracts (ethanol and water) on cryptosporidiosis in experimentally infected dexamethasone-immunosuppressed rats. METHODS: A total of 180 male rats (190-220) g BWt were randomly divided into 9 equal groups (G1-G9). Groups of rats were kept as (G1): normal control, (G2-G9): immunosuppressed with dexamethasone and (G3-G9): infected with Cryptosporidium oocysts. Rats from (G4-G9) were given orally ethanol and water extract of propolis (at a dose of 50 mg/kg BWt) and nitazoxanide (standard anti-cryptosporidial drug at a dose of 100 mg/kg BWt) to infected rats with different regimes...
March 2017: Asian Pacific Journal of Tropical Medicine
https://www.readbyqxmd.com/read/28441870/protein-nanocellulose-interactions-in-paper-filters-for-advanced-separation-applications
#4
Simon Johan Gustafsson, Levon Manukyan, Albert Mihranyan
Protein-based pharmaceutics are widely explored for healthcare applications, and six out of ten best selling drugs today are biologicals. The goal of this work was to evaluate the protein nanocellulose interactions in paper filter for advanced separation applications such as virus removal filtration and bioprocessing. The protein recovery was measured for bovine serum albumin (BSA), γ-Globulin and Lysozyme using biuret total protein reagent and polyacrylamide gel electrophoresis (SDS-PAGE), and the throughput was characterized in terms of flux values from fixed volume filtrations at various protein concentrations and under worst-case experimental conditions...
April 26, 2017: Langmuir: the ACS Journal of Surfaces and Colloids
https://www.readbyqxmd.com/read/28441796/role-of-dietary-crocin-in-in-vivo-melanoma-tumor-remission
#5
Hamid A Bakshi, Faruck Lukmanul Hakkim, Smitha Sam, Farideh Javid
Background: Melanoma is a deadly form of malignancy. Early diagnosis might pave the way to cure but its aggressive nature leads to rapid dissemination and colonization of distant organs. Dietary herbs may play a significant role in prevention of cancer. In this study, we tested anti-tumor efficacy of the Crocus sativus derived active constituent crocin, well established to have anti-cancer properties in different cancer models by our group and other groups and other groups. Notably, crocin is reported to exert anti-proliferative effect on melanoma cells (B16F10) in vitro...
March 1, 2017: Asian Pacific Journal of Cancer Prevention: APJCP
https://www.readbyqxmd.com/read/28438676/factors-associated-with-long-term-risk-of-relapse-after-unrelated-cord-blood-transplantation-for-children-with-acute-lymphoblastic-leukemia-in-remission
#6
Kristin M Page, Myriam Labopin, Annalisa Ruggeri, Gerard Michel, Cristina Diaz de Heredia, Tracey O'Brien, Alessandra Picardi, Mouhab Ayas, Henrique Bittencourt, Ajay J Vora, Jesse Troy, Carmen Bonfim, Fernanda Volt, Eliane Gluckman, Peter Bader, Joanne Kurtzberg, Vanderson Rocha
For pediatric patients with acute lymphoblastic leukemia (ALL), relapse is an important cause of treatment failure after unrelated cord blood transplant (UCBT). Compared to other donor sources, relapse is similar or even reduced after UCBT despite less graft-versus-host disease (GvHD). We performed a retrospective analysis to identify risk factors associated with the 5-year cumulative incidence (CI) of relapse after UCBT. In this retrospective, registry-based study, we examined the outcomes of 640 children (<18 years) with ALL in first (n=257, 40%) or second complete remission (CR; n=383, 60%) who received myeloablative conditioning followed by a single-unit UCBT from 2000-2012...
April 21, 2017: Biology of Blood and Marrow Transplantation
https://www.readbyqxmd.com/read/28438374/validity-of-self-assessed-sexual-maturation-against-physician-assessments-and-hormone-levels
#7
Jorge E Chavarro, Deborah J Watkins, Myriam C Afeiche, Zhenzhen Zhang, Brisa N Sánchez, David Cantonwine, Adriana Mercado-García, Clara Blank-Goldenberg, John D Meeker, Martha María Téllez-Rojo, Karen E Peterson
OBJECTIVE: To compare self-report and physician assessments of sexual maturation against serum hormone markers to evaluate the hypothesis that the validity of self-assessed sexual maturation is underestimated in traditional validation studies. STUDY DESIGN: We adapted a self-assessment instrument that 248 Mexican children and adolescents, aged 8-13 years, completed. The participants were examined by a trained pediatrician and provided fasting blood samples for measurement of reproductive hormones (eg, testosterone, estradiol, sex hormone-binding globulin, inhibin B) and other hormones (eg, C-peptide, insulin-like growth factor 1, leptin, dehydroepiandrosterone sulfate) known to change during adolescence...
April 21, 2017: Journal of Pediatrics
https://www.readbyqxmd.com/read/28436978/association-of-hematopoietic-cell-transplantation-specific-comorbidity-index-with-resource-utilization-after-allogeneic-transplantation
#8
L Decook, Y-H Chang, J Slack, D Gastineau, J Leis, P Noel, J Palmer, L Sproat, M Sorror, N Khera
Comorbidities affect clinical outcomes and costs in medicine. The hematopoietic cell transplantation (HCT)-specific comorbidity index (HCT-CI) predicts mortality risk after HCT. Its association with resource utilization (RU) is unknown. In this single-center, retrospective study, we examined the association of HCT-CI with RU (readmissions, length of hospital stay (LOS) and days out of hospital alive (DOHA)) in first 100 days (n=328) and 1 year (n=226) in allogeneic HCT patients from January 2010 to June 2014...
April 24, 2017: Bone Marrow Transplantation
https://www.readbyqxmd.com/read/28436338/the-effect-of-boswellia-serrata-resin-diet-supplementation-on-production-hematological-biochemical-and-immunological-parameters-in-broiler-chickens
#9
A R M Al-Yasiry, B Kiczorowska, W Samolińska, E Kowalczuk-Vasilev, D Kowalczyk-Pecka
Boswellia serrata resin (BSR), exhibiting a variety of therapeutic properties, is applied in Asian traditional medicine. These properties can be used in poultry production as well. Application of the resin as a phytobiotic in broiler chicken rearing can increase the productivity and improve meat quality. However, the optimum and maximum levels of BSR in broiler diets need to be assessed. The study determined the effect of different levels of supplementation of BSR (directly derived, unprocessed) in diets for broiler chickens on the production traits, selected slaughter analysis parameters, nutrient digestibility and selected hematological, biochemical and immunological parameters...
April 24, 2017: Animal: An International Journal of Animal Bioscience
https://www.readbyqxmd.com/read/28435998/a-6-year-old-boy-with-wilson-disease-a-diagnostic-dilemma
#10
Ramaswamy Ganesh, N Suresh, T Vasanthi, Malathi Sathiyasekaran, R Thulasiraman
A 6-year-old boy presented with 2 months history of progressive abdominal distension and jaundice. He was deeply icteric with ascites, hepatosplenomegaly, hyperbilirubinemia, raised transaminases, and coagulopathy. Viral markers and slit lamp examination for Kayser-Fleischer ring were negative. Serum ceruloplasmin and 24-h urinary copper post-D-pencillamine challenge were normal. Anti-smooth muscle antibody was positive 1:20, and liver biopsy showed micronodular cirrhosis with abundant Mallory hyaline and stainable copper deposits...
April 24, 2017: Indian Journal of Gastroenterology: Official Journal of the Indian Society of Gastroenterology
https://www.readbyqxmd.com/read/28435196/hemato-biochemical-alterations-and-urinalysis-in-dogs-suffering-from-benign-prostatic-hyperplasia
#11
M R Das, R C Patra, R K Das, P K Rath, B P Mishra
AIM: The study was designed to evaluate the hemato-biochemical alterations, urinalysis along with histomorphological and histological changes of prostate glands in dogs affected with benign prostatic hyperplasia (BPH) in and around Bhubaneswar, Odisha, India. MATERIALS AND METHODS: In toto, 445 dogs presented to the Teaching Veterinary Clinical Complex of the College of Veterinary Sciences and Animal Husbandry, one Government Veterinary Hospital and two pet clinics in and around Bhubaneswar screened for the presence of BPH...
March 2017: Veterinary World
https://www.readbyqxmd.com/read/28435195/intestinal-microbial-ecology-and-hematological-parameters-of-broiler-fed-cassava-waste-pulp-fermented-with-acremonium-charticola
#12
Sugiharto Sugiharto, Turrini Yudiarti, Isroli Isroli, Endang Widiastuti, Fatan Dwi Putra
AIM: The aim of this study was to evaluate the effect of feeding Acremonium charticola-fermented cassava pulp (AC-FCP) on the intestinal microbial ecology and hematological indices of broiler chickens. MATERIALS AND METHODS: A total of 240 male Lohman day-old-chicks were randomly allotted to one of the four experimental diets including control diet, control diet + antimicrobials (neomycin; 300 mg/kg diet), diet containing AC-FCP (16 g/100 g diet) or diet containing AC-FCP + antimicrobials...
March 2017: Veterinary World
https://www.readbyqxmd.com/read/28432801/weight-loss-in-obese-girls-with-polycystic-ovarian-syndrome-is-associated-with-a-decrease-of-anti-muellerian-hormone-concentrations
#13
Thomas Reinehr, Alexandra Kulle, Juliane Rothermel, Caroline Knop, Nina Lass, Christina Bosse, Paul-Martin Holterhus
OBJECTIVE: The Anti- Muellerian Hormone (AMH) has been reported as surrogate marker of antral follicles, which are the origins of hyperandrogenism in polycystic ovarian syndrome (PCOS). Therefore, AMH may be useful for the diagnosis of PCOS. The objective was to study the longitudinal changes in AMH concentrations in girls with and without PCOS. DESIGN: This is a longitudinal study of obese girls participating in a 1-year lifestyle intervention. PATIENTS: Forty obese girls aged 13-16 years (50% with PCOS) were included in the study...
April 22, 2017: Clinical Endocrinology
https://www.readbyqxmd.com/read/28427398/euphorbia-dracunculoides-l-abrogates-carbon-tetrachloride-induced-liver-and-dna-damage-in-rats
#14
Riffat Batool, Muhammad Rashid Khan, Muhammad Majid
BACKGROUND: Evaluation of Euphorbia dracunculoides of family Euphorbiaceae during previous studies had established the in vitro antioxidant and in vivo anti-inflammatory activities. The plant is used by the local communities of Pakistan for various disorders including rheumatism and edema. In this investigation we have evaluated the hepatoprotective effects against CCl4 induced toxicity in rat. METHODS: Dry powder of the aerial parts of E. dracunculoides was extracted with 95% methanol to get the extract (EDME)...
April 20, 2017: BMC Complementary and Alternative Medicine
https://www.readbyqxmd.com/read/28425408/testosterone-sex-hormone-binding-globulin-and-nonalcoholic-fatty-liver-disease-a-systematic-review-and-meta-analysis
#15
Veeravich Jaruvongvanich, Anawin Sanguankeo, Tanawan Riangwiwat, Sikarin Upala
INTRODUCTION AND AIM: Endogenous sex hormones are associated with the risk of diabetes and metabolic syndrome. Recent studies suggested the role of these hormones in nonalcoholic fatty liver disease (NAFLD). We conducted a systematic review and meta-analysis of observational studies investigating the association between sex hormones and NAFLD. MATERIAL AND METHODS: A comprehensive search of the databases of the MEDLINE and EMBASE was performed from inception through April 2016...
May 2017: Annals of Hepatology
https://www.readbyqxmd.com/read/28424782/clinical-significance-of-preoperative-albumin-and-globulin-ratio-in-patients-with-gastric-cancer-undergoing-treatment
#16
Min-Jie Mao, Xiao-Li Wei, Hui Sheng, Xue-Ping Wang, Xiao-Hui Li, Yi-Jun Liu, Shan Xing, Qi Huang, Shu-Qin Dai, Wan-Li Liu
Background. The pretreatment albumin and globulin ratio (AGR) was an inflammation-associated factor which was related to the overall survival in various malignancies. The aim of this study was to evaluate the prognostic value of AGR in patients with gastric cancer. Method. This retrospective study included 862 cases pathologically diagnosed with gastric cancer. All patients were randomly divided into the testing group (431 cases) and validation group (431 cases). The relationships of AGR with clinicopathologic characteristics and prognosis were analyzed by Kaplan-Meier and Cox regression methods...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28419525/method-comparison-and-generation-of-plasma-biochemistry-ris-for-the-white-rhinoceros-on-a-point-of-care-and-wet-chemistry-analyzer
#17
Emma H Hooijberg, Gerhard Steenkamp, Peter Buss, Amelia Goddard
BACKGROUND: Plasma biochemistry analysis may aid the management of White rhinoceros with poaching or other injuries. Analyzer- and species-specific RIs are necessary to evaluate health status and identify abnormalities. OBJECTIVES: The purpose of the study was to perform a comparison study between the IDEXX VetTest and Roche Cobas Integra 400 Plus, and to generate analyzer-specific RIs for White rhinoceros according to published guidelines. METHODS: Comparison was carried out using White rhinoceros plasma samples from healthy and diseased animals...
April 17, 2017: Veterinary Clinical Pathology
https://www.readbyqxmd.com/read/28419453/obstetrics-and-gynecology-physician-knowledge-of-rh-immune-globulin-prophylaxis
#18
Amy Yu, Erin Morris, Richard Adams, Mark K Fung
BACKGROUND: Previous studies have shown that more than 20% of laboratories would have recommended inaccurate doses of Rh immune globulin (RhIG) in hypothetical cases. Efforts have been made in educating laboratories in correct dosing calculations; however, obstetricians are most often responsible for ordering RhIG. The objective of this study was to assess knowledge of RhIG indications and dosing among obstetrics and gynecology (OB/GYN) physicians in the United States. STUDY DESIGN AND METHODS: An anonymous 17- question online survey was distributed to all OB/GYN resident and attending physicians affiliated with US residency training programs...
April 16, 2017: Transfusion
https://www.readbyqxmd.com/read/28419309/influence-of-dietary-inclusion-of-untreated-or-heat-treated-jatropha-meal-on-productive-and-reproductive-performances-and-biochemical-blood-parameters-of-laying-japanese-quail
#19
M E Abd El-Hack, M Alagawany, S A A El-Sayed, J Fowler
Jatropha meal (JM) has been characterized as a potential animal feedstuff due to its high crude protein content and high levels of essential amino acids. However, it contains anti-nutritive and toxic compounds that may hinder its use. The present study was conducted to evaluate the effects of a dietary inclusion of raw Jatropha meal (RJM) and heat-treated Jatropha meal on the growth and production of laying Japanese quail using productive, reproductive, and biochemical blood parameters. A total number of 180 mature Japanese quail at 2 months of age (120 females and 60 males) was randomly divided into 4 treatment groups: control (0% JM); 3...
April 17, 2017: Poultry Science
https://www.readbyqxmd.com/read/28415521/effect-of-halloysite-nanotubes-on-the-structure-and-function-of-important-multiple-blood-components
#20
Keke Wu, Ru Feng, Yanpeng Jiao, Changren Zhou
Many researchers have investigated the application of halloysite nanotubes (HNTs) in biomedicine, because of their special nanoscale hollow tubular structure. Although the cytocompatibility of HNTs has been studied, their blood compatibility has not been systematically investigated. In this work, the effect of HNTs on the structure and function of different blood components has been studied, including the morphology and hemolysis of red blood cells (RBCs). Based on scanning electron microscopy (SEM) observations, optical density test and flow cytometry analysis, we found that HNTs can affect the morphology and membrane integrity of RBCs in phosphate buffered saline (PBS) in a content-dependent way...
June 1, 2017: Materials Science & Engineering. C, Materials for Biological Applications
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