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dialyzable leukocyte extracts

Erika Evangelina Coronado-Cerda, Moisés Armides Franco-Molina, Edgar Mendoza-Gamboa, Heriberto Prado-García, Lydia Guadalupe Rivera-Morales, Pablo Zapata-Benavides, María Del Carmen Rodríguez-Salazar, Diana Caballero-Hernandez, Reyes Silvestre Tamez-Guerra, Cristina Rodríguez-Padilla
Chemotherapy treatments induce a number of side effects, such as leukopenia neutropenia, peripheral erythropenia, and thrombocytopenia, affecting the quality of life for cancer patients. 5-Fluorouracil (5-FU) is wieldy used as myeloablative model in mice. The bovine dialyzable leukocyte extract (bDLE) or IMMUNEPOTENT CRP® (ICRP) is an immunomodulatory compound that has antioxidants and anti-inflammatory effects. In order to investigate the chemoprotection effect of ICRP on bone marrow cells in 5-FU treated mice, total bone marrow (BM) cell count, bone marrow colony forming units-granulocyte/macrophage (CFU-GM), cell cycle, immunophenotypification, ROS/superoxide and Nrf2 by flow cytometry, and histological and hematological analyses were performed...
2016: Journal of Immunology Research
Nohemí Salinas-Jazmín, Sergio Estrada-Parra, Miguel Angel Becerril-García, Alberto Yairh Limón-Flores, Said Vázquez-Leyva, Emilio Medina-Rivero, Lenin Pavón, Marco Antonio Velasco-Velázquez, Sonia Mayra Pérez-Tapia
Human dialyzable leukocyte extracts (DLEs) are heterogeneous mixtures of low-molecular-weight peptides that are released on disruption of peripheral blood leukocytes from healthy donors. DLEs improve clinical responses in infections, allergies, cancer, and immunodeficiencies. Transferon is a human DLE that has been registered as a hemoderivate by Mexican health authorities and commercialized nationally. To develop an animal model that could be used routinely as a quality control assay for Transferon, we standardized and validated a murine model of cutaneous HSV-1 infection...
2015: Journal of Immunology Research
Uriel García-Hernández, Frank H Robledo-Avila, Violeta D Alvarez-Jiménez, Octavio Rodríguez-Cortés, Isabel Wong-Baeza, Jeanet Serafín-López, Luz M Aguilar-Anguiano, Sergio Estrada-Parra, Iris Estrada-García, Sonia M Pérez-Tapia, Rommel Chacón-Salinas
Dialyzable leukocyte extracts (DLE) transfer specific cell-mediated immune responses from sensitized donors to non-immune recipients. In addition, DLE have several immunomodulatory effects and are used for the treatment of several infectious and non-infectious diseases. Previous studies showed that human DLE obtained from virus-infected leukocytes and bovine DLE decrease the production of the pro-inflammatory cytokine TNF-alpha in response to bacterial lipopolysaccharide, in vitro and in vivo. In the present work, we inquire as to whether DLE from uninfected human leukocytes have the ability to regulate cytokine production in peripheral blood mononuclear cells (PBMC) in vitro...
June 2014: Natural Product Communications
María Eugenia Hernandez, Danelia Mendieta, Mayra Pérez-Tapia, Rafael Bojalil, Iris Estrada-Garcia, Sergio Estrada-Parra, Lenin Pavón
Major depressive disorder (MDD) is a psychiatric illness that presents as a deficit of serotonergic neurotransmission in the central nervous system. MDD patients also experience alterations in cortisol and cytokines levels. Treatment with selective serotonin reuptake inhibitors (SSRIs) is the first-line antidepressant regimen for MDD. The aim of this study was to determine the effect of a combination of SSRIs and an immunomodulator-human dialyzable leukocyte extract (hDLE)-on cortisol and cytokines levels. Patients received SSRIs or SSRIs plus hDLE...
2013: Clinical & Developmental Immunology
F Robledo-Ávila, M Pérez-Tapia, A Limón-Flores, L Pavon, R Hernández-Pando, I Wong-Baeza, G González-González, C Tovar, S Estrada-Parra, I Estrada-García
Candida albicans causes opportunistic systemic infections with high mortality (30%-50%). Despite significant nephrotoxicity, amphotericin (AmB) is still used for the treatment of this serious fungal infection. Therefore, alternative treatments are urgently needed. Dialyzable leukocyte extracts have been used successfully to treat patients with mucocutaneous candidiasis, but their effectiveness in systemic candidiasis has not been evaluated. In this study, low-dose AmB (0.1 mg/kg) plus 10 pg of murine dialyzable spleen extracts (mDSE) were tested in a systemic candidiasis mouse model...
2013: Clinical & Developmental Immunology
Emilio Medina-Rivero, Giovanna Merchand-Reyes, Lenin Pavón, Said Vázquez-Leyva, Gilberto Pérez-Sánchez, Nohemí Salinas-Jazmín, Sergio Estrada-Parra, Marco Velasco-Velázquez, Sonia Mayra Pérez-Tapia
Human dialyzable leukocyte extracts (DLEs) are heterogeneous mixtures of low-molecular-weight peptides that modulate immune responses in various diseases. Due their complexity, standardized methods to identify their physicochemical properties and determine that production batches are biologically active must be established. We aimed to develop and validate a size exclusion ultra performance chromatographic (SE-UPLC) method to characterize Transferon™, a DLE that is produced under good manufacturing practices (GMPs)...
January 2014: Journal of Pharmaceutical and Biomedical Analysis
Blanca María Morfin-Maciel, Julieta Margarita Sotelo-Ortiz
BACKGROUND: Human papillomavirus (HPV) is one of the most common sexually transmitted infections worldwide. Most HPV infections are cleared within two years by the immune system. Only in 5% to 10% of infected women the infection persists determining a high risk of developing cervical intraepithelial neoplasia. The transfer factor (TF) or dialyzable leukocyte extract is an immunomodulator that has been successfully used as an adjuvant in the treatment of intracelular infections such as recurrent herpes virus diseases...
July 2012: Revista Alergia Mexico: Organo Oficial de la Sociedad Mexicana de Alergia e Inmunología, A.C
Humberto H Lara, Liliana Ixtepan Turrent, Elsa N Garza-Treviño, Reyes Tamez-Guerra, Cristina Rodriguez-Padilla
Dialyzable leukocyte extract (DLE) is one of the immunological agents used as an adjuvant in cancer therapy; it has been associated with improved quality of life during cancer chemotherapy. Based on these previous findings and on the observed clinical benefits attributed to DLE in other types of cancer, we investigated its clinical and immunological effects as a therapy adjuvant on breast cancer patients who received only chemotherapy, as compared to patients administered bovine DLE (bDLE) as an adjuvant. This study included 43 breast cancer patients who were about to begin chemotherapy...
May 2010: Experimental and Therapeutic Medicine
Humberto H Lara, Liliana Ixtepan-Turrent, Elsa N Garza-Treviño, Jose I Badillo-Almaraz, Cristina Rodriguez-Padilla
BACKGROUND: Bovine dialyzable leukocyte extract (bDLE) is derived from immune leukocytes obtained from bovine spleen. DLE has demonstrated to reduce transcription of Human Immunodeficiency Virus Type 1 (HIV-1) and inactivate the nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling pathway. Therefore, we decided to clarify the mode of antiviral action of bDLE on the inhibition of HIV-1 infection through a panel of antiviral assays. RESULTS: The cytotoxicity, HIV-1 inhibition activity, residual infectivity of bDLE in HIV-1, time of addition experiments, fusion inhibition of bDLE for fusogenic cells and the duration of cell protection even after the removal of bDLE were all assessed in order to discover more about the mode of the antiviral action...
2011: BMC Research Notes
Atzin Robles-Contreras, Lizet Vizuet, Erika Rivera, Jeanet Serafin-López, Iris Estrada-Garcia, Sergio Estrada-Parra, Raúl Chávez, Yonathan Garfias, Mayra Perez-Tapia, María C Jiménez-Martínez
INTRODUCTION: Human Limbal Epithelial Cells (hLEC) are stem cells that give rise to corneal epithelium. After corneal damage, hLEC produce large amounts of IL-8 and IL-6, inducing inflammation in cornea and conjunctiva. Despite inflammation is necessary to repair the ocular surface since this process may be potentially harmful and could lead to corneal opacity. Ophthalmic infectious diseases have been treated with human dialyzable leukocyte extracts (hDLE). Clinical observations in hDLE-treated patients, have suggested an apparent control of ocular inflammatory injuries, without changes in the re-epithelialization process...
May 2011: Revista Alergia Mexico: Organo Oficial de la Sociedad Mexicana de Alergia e Inmunología, A.C
Javier Gómez Vera, Raúl Chávez Sánchez, Graciela Flores Sandoval, Modesto Orea Solano, José Jesús López Tiro, A D Santiago Santos, Sara Espinosa Padilla, Francisco Espinosa Rosales, J Huerta, José Antonio Ortega Martell, Renato Berrón Pérez, Alejandro Estrada García, Mayra Pérez Tapia, Azucena Rodríguez Flores, Ernestina Serrano Miranda, Oscar Pineda García, Consuelo Andaluz, Edgar Cervantes Trujano, Abraham Portugués Díaz, Javier Barrientos Zamudio, Laura Cano Ortíz, Jeanet Serafín López, María Del Carmen Jiménez Martínez, Gustavo Aguilar Velázquez, Yonathan Garfias Becerra, Concepción Santacruz Valdéz, Daniel Aguilar Ángeles, María Isabel Rojo Guiérrez, Miguel Aguilar Santelises, Sergio Estrada Parra
Although various mechanisms involving antibodies and various cell types participate, a Thl and Th2 cells imbalance seems to play a central role for allergy development. Other lymphocyte subpopulations, such as Th17, CD4 FOXP3, and Th9 positive regulatory T lymphocytes may also be involved in the allergic response. Regulatory processes are an appealing target for therapeutic approaches aiming to solve allergic reactions by restoring the delicate balance within the immune system. Transfer factor (TF) or dialyzable leukocyte extract is meant to transfer cell-mediated immunity from immune competent donors to unsensitized or deficient recipients...
November 2010: Revista Alergia Mexico: Organo Oficial de la Sociedad Mexicana de Alergia e Inmunología, A.C
M A Franco-Molina, E Mendoza-Gamboa, P Zapata-Benavides, M E Vera-García, P Castillo-Tello, A García de la Fuente, R D Mendoza, R G Garza, R S Támez-Guerra, C Rodríguez-Padilla
BACKGROUND: IMMUNEPOTENT CRP is a mixture of low molecular weight substances, some of which have been shown to be capable of modifying the immune response. We evaluated the response and adjuvant effect of IMMUNEPOTENT CRP on non-small cell lung cancer (NSCLC) patients in a phase I clinical trial. METHODS: Twenty-four NSCLC patients were included in the study and divided into two groups. Group 1 received a conventional treatment of 5400 cGy external radiotherapy in 28 fractions and chemotherapy consisting of intravenous cisplatin (40 mg/m(2)) delivered weekly for 6 weeks...
2008: Cytotherapy
E Mendoza-Gamboa, M A Franco-Molina, P Zapata-Benavides, P Castillo-Tello, M E Vera-García, R S Tamez-Guerra, C Rodríguez-Padilla
BACKGROUND: We have previously demonstrated that bovine dialyzable leukocyte extract (bDLE) induces death through an apoptosis mechanism in MCF-7 breast cancer cells. Depending on the cell type and stimulus, activating protein-1 (AP-1) has been shown to regulate cell proliferation and differentiation, the stress response, apoptosis and survival. It remains unknown whether AP-1 and other transcription factors are mechanisms by which bDLE induces cell death. METHODS: To determine whether bDLE modulates the AP-1 DNA binding and gene expression, MCF-7 breast cancer cells were treated with bDLE (0, 1, 5, 10 U) for 72 h and evaluated by electrophoretic mobility shift assay, reverse transcriptase-polymerase chain reaction and Western blot assays...
2008: Cytotherapy
M A Franco-Molina, E Mendoza-Gamboa, P Castillo-Tello, C E Isaza-Brando, M E Vera García, L Castillo-León, R S Tamez-Guerra, C Rodríguez-Padilla
BACKGROUND: In the current study, we determined whether bovine dialyzable leukocyte extract (bDLE) modulates lipopolysaccharide (LPS)-induced nitric oxide and cytokine overproduction. METHODS: Human whole blood cells were treated with LPS (50 ng) + bDLE (1 U). RESULTS: The bDLE treatment decreased nitric oxide as well as TNF-alpha, IL-6 and IL-10 (P <0.01) cytokine production. In addition, it decreased TNF-alpha, IL-1beta and IL-6 mRNA expression and suppressed IL-10 and IL-12p40 mRNA expression, but did not modulate IL-8 mRNA expression in LPS-stimulated human blood cells...
2007: Cytotherapy
I Abubakar, S H Aliyu, C Arumugam, P R Hunter, N K Usman
BACKGROUND: Cryptosporidiosis is a disease that causes diarrhoea lasting about one to two weeks, sometimes extending up to 2.5 months among the immunocompetent and becoming a more severe life-threatening illness among immunocompromised individuals. Cryptosporidium is a common cause of gastroenteritis. Cryptosporidiosis is common in HIV-infected individuals. OBJECTIVES: The objective of the review was to assess the efficacy of interventions for the treatment and prevention of cryptosporidiosis among immunocompromised individuals...
2007: Cochrane Database of Systematic Reviews
Moisés Armides Franco-Molina, Edgar Mendoza-Gamboa, Paloma Castillo-Tello, Reyes S Tamez-Guerra, Licet Villarreal-Treviño, Rolando Tijerina-Menchaca, Leonardo Castillo-León, Pablo Zapata-Benavides, Cristina Rodríguez-Padilla
The rapidly developing resistance of many infectious pathogenic organisms to modern drugs has spurred scientists to search for new sources of antibacterial compounds. One potential candidate, bDLE (dialysis at 10 to 12 kDa cut-off) and its fractions ("S" and "L" by 3.5 kDa cut-off and I, II, III, and IV by molecular exclusion chromatography), was evaluated for antibacterial activity against pathogenic bacterial strains (Staphylococcus aureus, Streptococcus pyogenes, Lysteria monocytogenes, Escherichia coli, Pseudomonas aeruginosa, and Salmonella typhi) using standard antimicrobial assays...
2006: Immunopharmacology and Immunotoxicology
M A Franco-Molina, E Mendoza-Gamboa, D Miranda-Hernández, P Zapata-Benavides, L Castillo-León, C Isaza-Brando, R S Tamez-Guerra, C Rodríguez-Padilla
BACKGROUND: Bovine dialyzable leukocyte extract (bDLE) is a dialyzate of a heterogeneous mixture of low molecular weight substances released from disintegrated blood leukocytes or lymphoid tissue obtained from homogenized bovine spleen. The purpose of this study was to determine if bDLE had cytotoxic effects and modulated apoptosis gene expression in breast cancer cells. METHODS: The MCF-7, BT-474, MDA-MB-453, A-427, Calu-1, U937 and L5178Y cancer cell lines and PBMC human cells were treated with bDLE (0-0...
2006: Cytotherapy
Yan Ping Xu, Wei Min Zou, Xue Jun Zhan, Shu Hua Yang, Da Ze Xie, Sai Liang Peng
To prepare an effective immune preparation to treat hepatitis B, hens were immunized with hepatitis B vaccines, and then anti-HBV egg yolk extraction (anti-HBV EYE) was refined from egg yolk by a dialyzable method. Its chemical characteristics were identified by ultraviolet spectrum, HPLC, Lowry analysis and pharmacopocia-raleted methods. The specific immunological activity was examined by leukocyte adherence inhibition (LAI) in vitro and delayed type hypersensitivity (DTH) in vivo. Anti-HBV EYE was a small dialyzable substance with molecular weight less than 12 kD containing 18 kinds of amino acids...
February 2006: Cellular & Molecular Immunology
Moisés A Franco-Molina, Edgar Mendoza-Gamboa, Leonardo Castillo-León, Reyes S Tamez-Guerra, Cristina Rodríguez-Padilla
Lipopolysaccharides (LPS) released from Gram-negative bacteria after infection initiate an exagerated response that leads to a cascade of pathophysiological events termed sepsis. Monocytes or macrophages produce many of the mediators found in septic patients. Targeting of these mediators, especially tumor necrosis factor (TNF)-alpha and nitric oxide (NO), has been pursued as a mean of reducing mortality in sepsis. Bovine dialyzable leukocyte extract (bDLE) is a dialysate of a heterogeneous mixture of low-molecular-weight substances released from disintegrated leukocytes of the blood or tissue lymphoid...
2005: Journal of Medicinal Food
Miriam Ojeda Ojeda, Cornelis van't Veer, Celia B Fernández Ortega, Manuel de J Araña Rosainz, Wim A Buurman
OBJECTIVE: To investigate i) whether the Dialyzable Leukocyte Extract (DLE) modulates the production of proinflammatory cytokines in leukocytes activated by the bacterial cell wall components lipopolysaccharide (LPS), lipoteichoic acid (LTA), and peptidoglycan (PGN); ii) the effect of DLE on LPS-stimulated endothelial cells; and iii) whether the regulatory effect of DLE on inflammatory mediators is related to the modulation of Toll-like receptors (TLRs), NF-kappaB and cAMP signaling pathways...
February 2005: Inflammation Research: Official Journal of the European Histamine Research Society ... [et Al.]
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