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https://www.readbyqxmd.com/read/27924792/a-cost-effective-approach-to-the-diagnosis-and-management-of-acute-infectious-encephalitis
#1
Usha Kant Misra, Vinita Elizabeth Mani, Jayantee Kalita
SETTING: A tertiary care teaching hospital in India. OBJECTIVE: To report a syndromic approach to acute encephalitis syndrome (AES) and propose a cost-effective model. STUDY DESIGN: AES patients were categorized by the presence or absence of myalgia/rash into systemic and neurological AES. The patients with systemic AES were investigated for dengue, scrub typhus, leptospira, chikungunya, and malaria, and those with neurological AES were investigated for herpes and Japanese encephalitis (JE)...
December 8, 2016: European Neurology
https://www.readbyqxmd.com/read/27923205/spontaneous-rupture-of-splenic-hematoma-in-a-malaria-patient-case-report-and-review-of-literature
#2
Bassem M Abou Hussein, Ali M Al Ani, Omar Al-Mayoofi, Mahpara Mehraj, Afra A Joher, Juanita A Bonilla, Alya S Al-Mazrouei, Faisal M Badri
INTRODUCTION: Blunt abdominal trauma is the most common cause of splenic rupture. Malaria is the most frequent tropical infectious cause of spontaneous splenic rupture. The exact mechanism is not well-defined. CASE REPORT: We report a case of thirty-year-old male patient known to have malaria who presented with spontaneous splenic rupture. A trial of conservative treatment failed and splenecomy was done to control bleeding. CONCLUSION: Spontaneous splenic rupture should be kept in mind in malaria patients presenting with left upper quadrant pain and signs of hypovolemia...
October 11, 2016: International Journal of Surgery Case Reports
https://www.readbyqxmd.com/read/27921101/the-effect-of-common-anticoagulants-in-detection-and-quantification-of-malaria-parasitemia-in-human-red-blood-cells-by-atr-ftir-spectroscopy
#3
Miguela Martin, David Perez-Guaita, Dean W Andrew, Jack S Richards, Bayden R Wood, Philip Heraud
Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR) has the potential to become a new diagnostic tool for malaria and other diseases. For point-of-care testing, the use of ATR-FTIR in malaria diagnosis enables the analysis of blood in the aqueous state, which represents an enormous advantage by minimising the sample preparation by removing the need for cell fixation. Here we report the quantification of malaria parasitemia in human RBCs in their normal physiological aqueous state...
December 6, 2016: Analyst
https://www.readbyqxmd.com/read/27920394/blood-transfusion-delay-and-outcome-in-county-hospitals-in-kenya
#4
Julius Thomas, Philip Ayieko, Morris Ogero, Susan Gachau, Boniface Makone, Wycliffe Nyachiro, George Mbevi, Mercy Chepkirui, Lucas Malla, Jacquie Oliwa, Grace Irimu, Mike English
Severe anemia is a leading indication for blood transfusion and a major cause of hospital admission and mortality in African children. Failure to initiate blood transfusion rapidly enough contributes to anemia deaths in sub-Saharan Africa. This article examines delays in accessing blood and outcomes in transfused children in Kenyan hospitals. Children admitted with nonsurgical conditions in 10 Kenyan county hospitals participating in the Clinical Information Network who had blood transfusion ordered from September 2013 to March 2016 were studied...
December 5, 2016: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27905280/diagnosis-and-species-discrimination-of-positive-malaria-samples-by-real-time-polymerase-chain-reaction
#5
Esra Atalay Şahar, Muhammet Karakavuk, Hüseyin Can, Şebnem Nergiz, Kadri Gül, Aysu Değirmenci Döşkaya, Yüksel Gürüz, Mert Döşkaya
OBJECTIVE: Malaria is an important tropical disease that is detected in 198 million people and causes 367-755 thousand deaths annually. Recently, the real-time Polymerase Chain Reaction (PCR) technique has enabled quick determination of Plasmodium spp. and species identification in the same assay with a low contamination risk. In the present study, we aimed to use real-time PCR targeting the 18S rRNA gene to diagnose Plasmodium spp. and perform species identification. METHODS: DNA samples of 15 patients with malaria (14 caused by P...
September 2016: Türkiye Parazitolojii Dergisi
https://www.readbyqxmd.com/read/27899111/plasmodium-malariae-in-the-colombian-amazon-region-you-don-t-diagnose-what-you-don-t-suspect
#6
Carlos Hernando Niño, Juan Ricardo Cubides, Paola Andrea Camargo-Ayala, Carlos Arturo Rodríguez-Celis, Teódulo Quiñones, Moisés Tomás Cortés-Castillo, Lizeth Sánchez-Suárez, Ricardo Sánchez, Manuel Elkin Patarroyo, Manuel Alfonso Patarroyo
BACKGROUND: Malaria is a worldwide public health problem; parasites from the genus Plasmodium spp. are the aetiological agent of this disease. The parasite is mainly diagnosed by microscope-based techniques. However, these have limited sensitivity. Many asymptomatic infections are sub-microscopic and can only be detected by molecular methods. This study was aimed at comparing nested PCR results to those obtained by microscope for diagnosing malaria and to present epidemiological data regarding malaria in Colombia's Amazon department...
November 29, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27895267/prototype-positive-control-wells-for-malaria-rapid-diagnostic-tests-prospective-evaluation-of-implementation-among-health-workers-in-lao-people-s-democratic-republic-and-uganda
#7
David Bell, John Baptist Bwanika, Jane Cunningham, Michelle Gatton, Iveth J González, Heidi Hopkins, Simon Peter S Kibira, Daniel J Kyabayinze, Mayfong Mayxay, Bbaale Ndawula, Paul N Newton, Koukeo Phommasone, Elizabeth Streat, René Umlauf
Rapid diagnostic tests (RDTs) are widely used for malaria diagnosis, but lack of quality control at point of care restricts trust in test results. Prototype positive control wells (PCW) containing recombinant malaria antigens have been developed to identify poor-quality RDT lots. This study assessed community and facility health workers' (HW) ability to use PCWs to detect degraded RDTs, the impact of PCW availability on RDT use and prescribing, and preferred strategies for implementation in Lao People's Democratic Republic (Laos) and Uganda...
November 28, 2016: American Journal of Tropical Medicine and Hygiene
https://www.readbyqxmd.com/read/27894353/provider-and-user-acceptability-of-intermittent-screening-and-treatment-for-the-control-of-malaria-in-pregnancy-in-malawi
#8
Deborah Almond, Mwayi Madanitsa, Victor Mwapasa, Linda Kalilani-Phiri, Jayne Webster, Feiko Ter Kuile, Lucy Paintain
BACKGROUND: Malaria in pregnancy is a major cause of adverse maternal and fetal outcomes. Intermittent preventive treatment with sulfadoxine-pyrimethamine (IPTp-SP) is one of the control strategies promoted by WHO. In response to mounting resistance to SP, intermittent screening and treatment (ISTp) has been proposed as an alternative. The objective of this study was to explore the acceptability of ISTp for health workers and pregnant women. METHODS: Semi-structured interviews of ten health workers and five focus group discussions of 38 women enrolled in an ongoing trial comparing IPTp-SP and ISTp with dihydroartemisinin-piperaquine (DP) were conducted at two antenatal clinics in rural Malawi...
November 28, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27893838/performance-evaluation-of-malaria-microscopists-at-defense-health-facilities-in-addis-ababa-and-its-surrounding-areas-ethiopia
#9
Tigist Yitbarek, Desalegn Nega, Geremew Tasew, Bineyam Taye, Kassu Desta
BACKGROUND: Blood film microscopy is the gold standard approach for malaria diagnosis, and preferred method for routine patient diagnosis in health facilities. However, the inability of laboratory professionals to correctly detect and identify malaria parasites microscopically leads to an inappropriate administration of anti-malarial drugs to the patients and incorrect findings in research areas. This study was carried out to evaluate the performance of laboratory professionals in malaria diagnosis in health facilities under the Defense Health Main Department in Addis Ababa and its surroundings, Ethiopia...
2016: PloS One
https://www.readbyqxmd.com/read/27888627/identification-of-differentially-expressed-genes-in-the-development-of-osteosarcoma-using-rna-seq
#10
Yihao Yang, Ya Zhang, Xin Qu, Junfeng Xia, Dongqi Li, Xiaojuan Li, Yu Wang, Zewei He, Su Li, Yonghong Zhou, Lin Xie, Zuozhang Yang
OBJECTIVE: Osteosarcoma (OS) is a malignant bone tumor with high morbidity in young adults and adolescents. This study aimed to discover potential early diagnosis biomarkers in OS. RESULTS: In total, 111 differentially expressed genes (DEGs) were identified in primary OS compared with normal controls and 235 DEGs were identified in metastatic OS compared with primary OS. AURKB and PPP2R2B were the significantly up-regulated and down-regulated hub proteins, respectively, in the PPI protein-protein network (PPI) network of primary OS...
November 24, 2016: Oncotarget
https://www.readbyqxmd.com/read/27882478/unexpected-hosts-imaging-parasitic-diseases
#11
REVIEW
Pablo Rodríguez Carnero, Paula Hernández Mateo, Susana Martín-Garre, Ángela García Pérez, Lourdes Del Campo
Radiologists seldom encounter parasitic diseases in their daily practice in most of Europe, although the incidence of these diseases is increasing due to migration and tourism from/to endemic areas. Moreover, some parasitic diseases are still endemic in certain European regions, and immunocompromised individuals also pose a higher risk of developing these conditions. This article reviews and summarises the imaging findings of some of the most important and frequent human parasitic diseases, including information about the parasite's life cycle, pathophysiology, clinical findings, diagnosis, and treatment...
November 23, 2016: Insights Into Imaging
https://www.readbyqxmd.com/read/27881136/community-health-workers-adherence-to-referral-guidelines-evidence-from-studies-introducing-rdts-in-two-malaria-transmission-settings-in-uganda
#12
Sham Lal, Richard Ndyomugenyi, Lucy Paintain, Neal D Alexander, Kristian S Hansen, Pascal Magnussen, Daniel Chandramohan, Siân E Clarke
BACKGROUND: Many malaria-endemic countries have implemented national community health worker (CHW) programmes to serve remote populations that have poor access to malaria diagnosis and treatment. Despite mounting evidence of CHWs' ability to adhere to malaria rapid diagnostic tests (RDTs) and treatment guidelines, there is limited evidence whether CHWs adhere to the referral guidelines and refer severely ill children for further management. In southwest Uganda, this study examined whether CHWs referred children according to training guidelines and described factors associated with adherence to the referral guideline...
November 24, 2016: Malaria Journal
https://www.readbyqxmd.com/read/27875528/effectiveness-of-seasonal-malaria-chemoprevention-in-children-under-ten-years-of-age-in-senegal-a-stepped-wedge-cluster-randomised-trial
#13
Badara Cissé, El Hadj Ba, Cheikh Sokhna, Jean Louis NDiaye, Jules F Gomis, Yankhoba Dial, Catherine Pitt, Mouhamed NDiaye, Matthew Cairns, Ernest Faye, Magatte NDiaye, Aminata Lo, Roger Tine, Sylvain Faye, Babacar Faye, Ousmane Sy, Lansana Konate, Ekoue Kouevijdin, Clare Flach, Ousmane Faye, Jean-Francois Trape, Colin Sutherland, Fatou Ba Fall, Pape M Thior, Oumar K Faye, Brian Greenwood, Oumar Gaye, Paul Milligan
BACKGROUND: Seasonal Malaria Chemoprevention (SMC) with sulfadoxine-pyrimethamine (SP) plus amodiaquine (AQ), given each month during the transmission season, is recommended for children living in areas of the Sahel where malaria transmission is highly seasonal. The recommendation for SMC is currently limited to children under five years of age, but, in many areas of seasonal transmission, the burden in older children may justify extending this age limit. This study was done to determine the effectiveness of SMC in Senegalese children up to ten years of age...
November 2016: PLoS Medicine
https://www.readbyqxmd.com/read/27864679/prescription-of-antimalarial-drugs-in-the-pediatric-ward-of-the-national-hospital-of-lamord%C3%A3-in-niamey
#14
A Soumana, M Kamaye, B Issofou, H Dima, B Daouda, O N'Diaye, G Sall
In Niger, malaria is a major public health problem, due to the high number of deaths that are attributable to it and because of its heavy weight and socioeconomic status. The objective of this study was to contribute to the inventory of medical practices to rationalize the use of medicines and minimize the resistance phenomenon. This is a prospective study, which took place from May 1 to July 31, 2009, in the pediatric ward of the National Hospital of Lamordé. It concerned children aged from 0 to 14 years hospitalized and having received one or more antimalarial drugs during at least 24 h...
November 18, 2016: Bulletin de la Société de Pathologie Exotique
https://www.readbyqxmd.com/read/27861675/evaluation-of-the-completeness-and-timeliness-of-malaria-reporting-in-the-brazilian-amazon-2003-2012
#15
Rui Moreira Braz, Pedro Luiz Tauil, Ana Carolina Faria E Silva Santelli, Cor Jesus Fernandes Fontes
OBJECTIVE: to evaluate the completeness and timeliness of malaria case reporting, diagnosis and treatment in the Brazilian Amazon. METHODS: this is a descriptive study using data from 23 fields of notification forms recorded on the Malaria Epidemiological Surveillance Information System (Sivep-Malaria) between 2003 and 2012. RESULTS: data completeness was good in 86.0% of fields (≥90.0% filled in); there was timely recording of 40.6% of notifications at the Municipal Health Departments (0-7 days following notification) and 75...
January 2016: Epidemiologia e Servicos de Saude: Revista do Sistema Unico de Saude do Brasil
https://www.readbyqxmd.com/read/27857610/predictors-of-specific-anti-brucella-antibodies-among-humans-in-agro-pastoral-communities-in-sengerema-district-mwanza-tanzania-the-need-for-public-awareness
#16
Elifuraha B Mngumi, Mariam M Mirambo, Sospeter Wilson, Stephen E Mshana
BACKGROUND: Human brucellosis remains to be a neglected zoonotic disease among agro-pastoral communities where livestock rearing is one of the main economic activities. This study was conducted in different agro-pastoral communities in Sengerema district, Mwanza, Tanzania, to determine seroprevalence and predictors of anti-Brucella antibodies, information that may influence public awareness on the risk factors and strategies to improve the diagnosis of brucellosis in developing countries...
2016: Tropical Medicine and Health
https://www.readbyqxmd.com/read/27855644/febrile-illness-diagnostics-and-the-malaria-industrial-complex-a-socio-environmental-perspective
#17
Justin Stoler, Gordon A Awandare
BACKGROUND: Global prioritization of single-disease eradication programs over improvements to basic diagnostic capacity in the Global South have left the world unprepared for epidemics of chikungunya, Ebola, Zika, and whatever lies on the horizon. The medical establishment is slowly realizing that in many parts of sub-Saharan Africa (SSA), particularly urban areas, up to a third of patients suffering from acute fever do not receive a correct diagnosis of their infection. MAIN BODY: Malaria is the most common diagnosis for febrile patients in low-resource health care settings, and malaria misdiagnosis has soared due to the institutionalization of malaria as the primary febrile illness of SSA by international development organizations and national malaria control programs...
November 17, 2016: BMC Infectious Diseases
https://www.readbyqxmd.com/read/27852705/non-treatment-of-children-with-community-health-worker-diagnosed-fast-breathing-pneumonia-in-rural-malawi-exploratory-subanalysis-of-a-prospective-cohort-study
#18
Carina King, Tim Colbourn, Limangeni Mankhambo, James Beard, Debbie C Hay Burgess, Anthony Costello, Rasa Izadnegahdar, Norman Lufesi, Charles Mwansambo, Bejoy Nambiar, Eric S Johnson, Robert W Platt, David Mukanga, Eric D McCollum
BACKGROUND: Despite recent progress, pneumonia remains the largest infectious killer of children globally. This paper describes outcomes of not treating community-diagnosed fast-breathing pneumonia on patient recovery. METHODS: We conducted an exploratory subanalysis of an observational prospective cohort study in Malawi. We recruited children (2-59 months) diagnosed by community health workers with fast-breathing pneumonia using WHO integrated community case management (iCCM) guidelines...
November 16, 2016: BMJ Open
https://www.readbyqxmd.com/read/27847373/a-multicenter-pivotal-clinical-trial-of-a-urine-malaria-test-umt-for-the-rapid-diagnosis-of-plasmodium-falciparum-malaria
#19
Wellington A Oyibo, Nnenna Ezeigwe, Godwin Ntadom, Oladipo O Oladosu, Kaitlin Rainwater, Wendy O'Meara, Evaezi Okpokoro, William Brieger
BACKGROUND: The need to expand malaria diagnosis alongside policy requirements for mandatory testing before treatment motivates exploration of non-invasive rapid diagnostic tests (RDTs). We report the outcome of the first cross-sectional, single-blind clinical performance evaluation of a Urine Malaria Test (UMT) for Plasmodium falciparum (Pf) malaria diagnosis in febrile patients. METHODS: Matched urine and fingerprick blood from participants ≥2 years with fever (axillary temperature ≥37...
November 9, 2016: Journal of Clinical Microbiology
https://www.readbyqxmd.com/read/27847257/new-trend-in-the-epidemiology-of-thalassaemia
#20
REVIEW
Chi-Kong Li
Thalassaemia is the most common monogenic disorder worldwide. It is common in areas with prevalent malaria as thalassaemic red cells provide immunity against the parasite. The incidence of thalassaemia carriers is high in regions such as Mediterranean, Middle East, Indian subcontinent, Southeast Asia and South China. In the past few decades, migrants from the thalassaemia prevalent countries to non-prevalent countries, mainly North America and Central and North Europe, are rapidly increasing in number. The non-prevalent countries may not have established pre-natal screening system for thalassaemia...
October 26, 2016: Best Practice & Research. Clinical Obstetrics & Gynaecology
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