keyword
MENU ▼
Read by QxMD icon Read
search

factor deficiency

keyword
https://www.readbyqxmd.com/read/28944822/investigation-of-crucial-genes-and-micrornas-in-conventional-osteosarcoma-using-gene-expression-profiling-analysis
#1
Chuangang Peng, Qi Yang, Bo Wei, Baoming Yuan, Yong Liu, Yuxiang Li, Dawer Gu, Guochao Yin, Bo Wang, Dehui Xu, Xuebing Zhang, Daliang Kong
The present study aimed to screen potential genes associated with conventional osteosarcoma (OS) and obtain further information on the pathogenesis of this disease. The microarray dataset GSE14359 was downloaded from the Gene Expression Omnibus. A total of 10 conventional OS samples and two non‑neoplastic primary osteoblast samples in the dataset were selected to identify the differentially expressed genes (DEGs) using the Linear Models for Microarray Data package. The potential functions of the DEGs were predicted using Gene Ontology (GO) and pathway enrichment analyses...
September 18, 2017: Molecular Medicine Reports
https://www.readbyqxmd.com/read/28944344/irf4-couples-anabolic-metabolism-to-th1-cell-fate-determination
#2
Radomir Kratchmarov, Simone A Nish, Wen-Hsuan W Lin, William C Adams, Yen-Hua Chen, Bonnie Yen, Nyanza J Rothman, Ulf Klein, Steven L Reiner
Anabolic metabolism in lymphocytes promotes plasmablast and cytotoxic T cell differentiation at the expense of self-renewal. Heightened expression and function of the transcription factor IFN regulatory factor 4 (IRF4) accompany enhanced anabolic induction and full commitment to functional differentiation in B cells and CD8(+) T cells. In this study, we used a genetic approach to determine whether IRF4 plays an analogous role in Th1 cell induction. Our findings indicate that IRF4 promotes determined Th1 cell differentiation in tandem with anabolic metabolism of CD4(+) T cells...
September 1, 2017: Immunohorizons
https://www.readbyqxmd.com/read/28944071/prevalence-and-screening-for-anaemia-in-mild-to-moderate-crohn-s-disease-and-ulcerative-colitis-in-the-united-states-2010-2014
#3
Eboselume Akhuemonkhan, Alyssa Parian, Kay Miller, Stephen Hanauer, Susan Hutfless
BACKGROUND AND AIMS: Anaemia affects up to 74% patients with Crohn's disease (CD) and ulcerative colitis (UC) and is correlated with decreased quality of life. The European Crohn's and Colitis Organisation (ECCO) recommends at least annual screening for iron-deficiency anaemia. We aimed to determine the prevalence of anaemia, frequency of anaemia screening and factors associated with anaemia in a retrospective study of mild to moderate inflammatory bowel disease (IBD) in the USA. METHODS: Adults with at least two outpatient encounters for IBD between 2010 through 2014 who contributed laboratory information were identified from MarketScan, a US commercial claims database...
2017: BMJ Open Gastroenterology
https://www.readbyqxmd.com/read/28943310/asf1a-promotes-non-homologous-end-joining-repair-by-facilitating-phosphorylation-of-mdc1-by-atm-at-double-strand-breaks
#4
Kyung Yong Lee, Jun-Sub Im, Etsuko Shibata, Anindya Dutta
Double-strand breaks (DSBs) of DNA in eukaryotic cells are predominantly repaired by non-homologous end joining (NHEJ). The histone chaperone anti-silencing factor 1a (ASF1a) interacts with MDC1 and is recruited to sites of DSBs to facilitate the interaction of phospho-ATM with MDC1 and phosphorylation of MDC1, which are required for the recruitment of RNF8/RNF168 histone ubiquitin ligases. Thus, ASF1a deficiency reduces histone ubiquitination at DSBs, decreasing the recruitment of 53BP1, and decreases NHEJ, rendering cells more sensitive to DSBs...
September 13, 2017: Molecular Cell
https://www.readbyqxmd.com/read/28943254/sox7-is-required-for-muscle-satellite-cell-development-and-maintenance
#5
Rashida F Rajgara, Neena Lala-Tabbert, François Marchildon, Émilie Lamarche, Jennifer K MacDonald, Daryl A Scott, Alexandre Blais, Ilona S Skerjanc, Nadine Wiper-Bergeron
Satellite cells are skeletal-muscle-specific stem cells that are activated by injury to proliferate, differentiate, and fuse to enable repair. SOX7, a member of the SRY-related HMG-box family of transcription factors is expressed in quiescent satellite cells. To elucidate SOX7 function in skeletal muscle, we knocked down Sox7 expression in embryonic stem cells and primary myoblasts and generated a conditional knockout mouse in which Sox7 is excised in PAX3(+) cells. Loss of Sox7 in embryonic stem cells reduced Pax3 and Pax7 expression...
September 18, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28943250/in%C3%A2-vivo-generation-of-engraftable-murine-hematopoietic-stem-cells-by-gfi1b-c-fos-and-gata2-overexpression-within-teratoma
#6
Masao Tsukada, Yasunori Ota, Adam C Wilkinson, Hans J Becker, Motomi Osato, Hiromitsu Nakauchi, Satoshi Yamazaki
Generation of hematopoietic stem cells (HSCs) from pluripotent stem cells (PSCs) could potentially provide unlimited HSCs for clinical transplantation, a curative treatment for numerous blood diseases. However, to date, bona fide HSC generation has been largely unsuccessful in vitro. We have previously described proof of concept for in vivo HSC generation from PSCs via teratoma formation. However, our first-generation system was complex and the output low. Here, we further optimize this technology and demonstrate the following: (1) simplified HSC generation using transcription factor overexpression; (2) improved HSC output using c-Kit-deficient host mice, and (3) that teratomas can be transplanted and cryopreserved...
September 8, 2017: Stem Cell Reports
https://www.readbyqxmd.com/read/28942944/death-receptor-3-regulates-distinct-pathological-attributes-of-acute-versus-chronic-murine-allergic-lung-inflammation
#7
Ravinder Kaur Singh, William Victor Perks, Jason Peter Twohig, Emma J Kidd, Kenneth Broadley, Stuart N Farrow, Anwen Sian Williams, Philip Russel Taylor, Eddie Chung Yern Wang
The Death Receptor 3 (DR3)/Tumour Necrosis Factor-like cytokine 1A (TL1A) axis stimulates effector T cells and type 2 innate lymphocytes (ILC2) that trigger cytokine release and drive disease pathology in several inflammatory and autoimmune diseases, including murine models of acute allergic lung inflammation (ALI). The aim of this study was to elucidate the role of DR3 in chronic ALI compared to acute ALI, using mice genetically deficient in the DR3 gene (DR3(ko)). Results showed DR3 expression in the lungs of wild-type mice was up-regulated following induction of acute ALI and this increased expression was maintained in chronic disease...
September 15, 2017: Cellular Immunology
https://www.readbyqxmd.com/read/28942514/intrauterine-hyperglycemia-induced-inflammatory-signalling-via-the-receptor-for-advanced-glycation-end-products-in-the-cardiac-muscle-of-the-infants-of-diabetic-mother-rats
#8
Ritsuko Kawaharada, Haruna Masuda, Zhenyi Chen, Eric Blough, Tomoko Kohama, Akio Nakamura
PURPOSE: Gestational diabetes is associated with increased risk to the health of the mother and her offspring. In particular, the infants of diabetic mothers (IDMs) exhibit elevated levels of preterm birth, macrosomia, hypoglycemia, hypocalcemia, and cardiomyopathy. We have previously reported that IDMs showed abnormalities in cardiac Akt-related insulin signalling, and that these deficiencies in Akt-related signalling were attenuated by supplementing the maternal diet with fish-oil. Herein, we investigated whether the eicosapentaenoic acid (EPA) found in fish oil can be used to attenuate diabetes associated impairments in cardiomyocyte signalling...
September 23, 2017: European Journal of Nutrition
https://www.readbyqxmd.com/read/28942469/early-versus-late-diagnosis-of-complement-factor-i-deficiency-clinical-consequences-illustrated-in-two-families-with-novel-homozygous-cfi-mutations
#9
Clara Franco-Jarava, Elena Álvarez de la Campa, Xavier Solanich, Francisco Morandeira-Rego, Virgínia Mas-Bosch, Marina García-Prat, Xavier de la Cruz, Andrea Martín-Nalda, Pere Soler-Palacín, Manuel Hernández-González, Roger Colobran
The complement system is an important effector arm of innate immunity and plays a crucial role in the defense against common pathogens. But effective defense and maintenance of homeostasis requires a careful balance between complement activation and regulation. Factor I (FI) is one of the most important regulators of the complement system. Complete FI deficiency is a rare autosomal recessive disorder typically resulting in severe, recurrent infection by encapsulated bacteria. In the present study, we describe two patients from unrelated families with complete FI deficiency diagnosed at very different ages: Patient 1 is a 60-year-old man who had experienced several severe infections (pneumonia, meningitis, sepsis) since childhood, one of which caused significant and permanent neurologic sequelae...
September 23, 2017: Journal of Clinical Immunology
https://www.readbyqxmd.com/read/28942441/the-clinical-characteristics-and-prognostic-analysis-of-status-epilepticus-in-northeast-china
#10
Lichao Sun, Chuntao Han, Weihong Lin
OBJECTIVE: Status epilepticus (SE) is a neurological emergency that may cause severe neurological deficiency and even death. The aim of this study was to analyze the clinical characteristics and prognosis of SE in northeast China. Additionally, the etiology and classification are discussed. MATERIALS AND METHODS: We retrospectively collected the clinical profiles of patients diagnosed with SE in the Epilepsy Center of Jilin University between January 2011 and May 2015...
September 22, 2017: European Neurology
https://www.readbyqxmd.com/read/28941857/a-cross-sectional-study-to-assess-the-stigma-associated-with-tuberculosis-among-tuberculosis-patients-in-udupi-district-karnataka
#11
R Shivapujimath, A P Rao, A R Nilima, D M Shilpa
BACKGROUND: For decades, tuberculosis and other communicable diseases like human immunodeficiency virus/acquired immune deficiency syndrome, leprosy, etc., have been associated with stigma and discrimination by the society; this can interfere with the lifestyle and disease management among these patients. OBJECTIVE: To assess the stigma experienced by tuberculosis patients and to find the factors associated with stigma. METHODS: A cross-sectional study was conducted among 209 sputum-positive and sputum-negative tuberculosis patients...
October 2017: Indian Journal of Tuberculosis
https://www.readbyqxmd.com/read/28941022/endoglin-in-human-liver-disease-and-murine-models-of-liver-fibrosis-a-protective-factor-against-liver-fibrosis
#12
Muhammad Alsamman, Viktor Sterzer, Steffen Klaus Meurer, Hacer Sahin, Ute Schäper, Deniz Kuscuoglu, Pavel Strnad, Ralf Weiskirchen, Christian Trautwein, David Scholten
BACKGROUND & AIMS: Liver fibrosis is the outcome of chronic liver injury. TGF-β is a major profibrogenic cytokine modulating hepatic stellate cell (HSC) activation and extracellular matrix homeostasis. This study analyzes the effect of Endoglin (Eng), a TGF-β type III auxiliary receptor, on fibrogenesis in two models of liver injury by HSC specific endoglin deletion. METHODS: Eng expression was measured in human and murine samples of liver injury. After generating (GFAPC)(re(+)) Eng(Δ)(HSC) mice the impact of Endoglin deletion on chronic liver fibrosis was analyzed...
September 20, 2017: Liver International: Official Journal of the International Association for the Study of the Liver
https://www.readbyqxmd.com/read/28940958/a-novel-neurog3-mutation-in-neonatal-diabetes-associated-with-a-neuro-intestinal-syndrome
#13
Suna Hancili, Amélie Bonnefond, Julien Philippe, Emmanuel Vaillant, Franck De Graeve, Olivier Sand, Kanetee Busiah, Jean-Jacques Robert, Michel Polak, Philippe Froguel, Ayla Güven, Martine Vaxillaire
Neonatal diabetes mellitus (NDM) is a rare form of non-autoimmune diabetes usually diagnosed in the first 6 months of life. Various genetic defects have been shown to cause NDM with diverse clinical presentations and variable severity. Among transcriptional factor genes associated with isolated or syndromic NDM, a few cases of homozygous mutations in the NEUROG3 gene have been reported, all mutated patients presenting with congenital malabsorptive diarrhea with or without diabetes at a variable age of onset from early life to childhood...
September 22, 2017: Pediatric Diabetes
https://www.readbyqxmd.com/read/28940692/dpff-1-transcription-factor-deficiency-causes-the-aberrant-activation-of-mpk-1-and-meiotic-defects-in-the-caenorhabditis-elegans-germline
#14
Emmanuel Villanueva-Chimal, Laura S Salinas, Laura P Fernández-Cardenas, Gabriela Huelgas-Morales, Alejandro Cabrera-Wrooman, Rosa E Navarro
The d4 family of transcription factors consists of three members in mammals. DPF1/neuro-d4 is expressed mainly in neurons and the peripheral nervous system, and is important for brain development. DPF2/requiem/ubi-d4 is expressed ubiquitously and presumably functions as an apoptotic factor, especially during the deprivation of trophic factors. DPF3/cer-d4 is expressed in neurons and in the heart, and is important for heart development and function in zebrafish. In Drosophila, there is only one member, dd4, whose function is still unknown, but it is expressed in many tissues and is particularly abundant in the brain of developing embryos and in adults...
September 23, 2017: Genesis: the Journal of Genetics and Development
https://www.readbyqxmd.com/read/28940183/an-increased-cd25-positive-intestinal-regulatory-t-lymphocyte-population-is-dependent-on-cox-2-activity-in-the-apc-min-model
#15
Olusola O Faluyi, Paul Fitch, Sarah E M Howie
Only mismatch repair (MMR)-deficient colorectal cancer (CRC) appears to respond well to PD-1 inhibition at the present time. Emerging evidence suggests a role for micro-environmental factors such as CD25+ve cells modulating response to PD-1 inhibition. In the Apc(Min/+) model of familial adenomatous polyposis (MMR-proficient CRC), increased Cyclooxygenase-2 (Cox-2) expression by cells which include alternatively-activated mononuclear phagocytes promotes intestinal tumorigenesis by mechanisms which may include immune suppression...
September 23, 2017: Clinical and Experimental Immunology
https://www.readbyqxmd.com/read/28940047/epidemiology-of-cerebellar-diseases-and-therapeutic-approaches
#16
REVIEW
Michael S Salman
Diseases involving the cerebellum occur relatively commonly in children and adults around the globe. Many factors influence their epidemiology including geography, ethnicity, consanguinity, and the methodology used to ascertain patients. In addition, reliable epidemiological data rely heavily on accurate disease classification. Continuous advances in genetic research and neuroimaging modalities have resulted in improved understanding of cerebellar diseases and have led to several revisions in their classification...
September 22, 2017: Cerebellum
https://www.readbyqxmd.com/read/28939975/ketamine-induced-glutamatergic-mechanisms-of-sleep-and-wakefulness-insights-for-developing-novel-treatments-for-disturbed-sleep-and-mood
#17
Wallace C Duncan, Elizabeth D Ballard, Carlos A Zarate
Ketamine, a drug with rapid antidepressant effects and well-described effects on slow wave sleep (SWS), is a useful intervention for investigating sleep-wake mechanisms involved in novel therapeutics. The drug rapidly (within minutes to hours) reduces depressive symptoms in individuals with major depressive disorder (MDD) or bipolar disorder (BD), including those with treatment-resistant depression. Ketamine treatment elevates extracellular glutamate in the prefrontal cortex. Glutamate, in turn, plays a critical role as a proximal element in a ketamine-initiated molecular cascade that increases synaptic strength and plasticity, which ultimately results in rapidly improved mood...
September 23, 2017: Handbook of Experimental Pharmacology
https://www.readbyqxmd.com/read/28939776/phosphomimetic-s3d-cofilin-binds-but-only-weakly-severs-actin-filaments
#18
W Austin Elam, Wenxiang Cao, Hyeran Kang, Andrew Huehn, Glen M Hocky, Ewa Prochniewicz, Anthony C Schramm, Karina Negrón, Jean Garcia, Teresa T Bonello, Peter W Gunning, David D Thomas, Greg A Voth, Charles V Sindelar, Enrique M De La Cruz
Many biological processes, including cell division, growth, and motility, rely on rapid remodeling of the actin cytoskeleton and on actin-filament severing by the regulatory protein cofilin. Phosphorylation of vertebrate cofilin at serine 3 (Ser-3) regulates both actin binding and severing. Substitution of serine with aspartate at position 3 (S3D) is widely used to mimic cofilin phosphorylation in cells and in vitro. The S3D substitution weakens cofilin binding to filaments, and it is presumed that subsequent reduction in cofilin occupancy inhibits filament severing, but this hypothesis has remained untested...
September 22, 2017: Journal of Biological Chemistry
https://www.readbyqxmd.com/read/28939681/cic-loss-promotes-gliomagenesis-via-aberrant-neural-stem-cell-proliferation-and-differentiation
#19
Rui Yang, Lee H Chen, Landon J Hansen, Austin B Carpenter, Casey J Moure, Heng Liu, Christopher J Pirozzi, Bill H Diplas, Matthew S Waitkus, Paula K Greer, Huishan Zhu, Roger E McLendon, Darell D Bigner, Yiping He, Hai Yan
Inactivating mutations in the transcriptional repression factor Capicua (CIC) occur in ~50% of human oligodendrogliomas (OD), but mechanistic links to pathogenesis are unclear. To address this question, we generated Cic-deficient mice and human OD cell models. Genetic deficiency in mice resulted in a partially penetrant embryonic or perinatal lethal phenotype, with the production of an aberrant proliferative neural population in surviving animals. In vitro cultured neural stem cells derived from Cic conditional knockout mice bypassed an EGF requirement for proliferation and displayed a defect in their potential for oligodendrocyte differentiation...
September 22, 2017: Cancer Research
https://www.readbyqxmd.com/read/28939608/the-type-ix-secretion-system-is-required-for-virulence-of-the-fish-pathogen-flavobacterium-columnare
#20
Nan Li, Yongtao Zhu, Benjamin R LaFrentz, Jason P Evenhuis, David W Hunnicutt, Rachel A Conrad, Paul Barbier, Connor W Gullstrand, Jack E Roets, Jonathan L Powers, Surashree S Kulkarni, Devon H Erbes, Julio C García, Pin Nie, Mark J McBride
Flavobacterium columnare, a member of the phylum Bacteroidetes, causes columnaris disease in wild and aquaculture-reared freshwater fish. The mechanisms responsible for columnaris disease are not known. Many members of the phylum Bacteroidetes use type IX secretion systems (T9SSs) to secrete enzymes, adhesins, and proteins involved in gliding motility. The F. columnare genome has all of the genes needed to encode a T9SS. gldN, which encodes a core component of the T9SS, was deleted in wild type strains of F...
September 22, 2017: Applied and Environmental Microbiology
keyword
keyword
77729
1
2
Fetch more papers »
Fetching more papers... Fetching...
Read by QxMD. Sign in or create an account to discover new knowledge that matter to you.
Remove bar
Read by QxMD icon Read
×

Search Tips

Use Boolean operators: AND/OR

diabetic AND foot
diabetes OR diabetic

Exclude a word using the 'minus' sign

Virchow -triad

Use Parentheses

water AND (cup OR glass)

Add an asterisk (*) at end of a word to include word stems

Neuro* will search for Neurology, Neuroscientist, Neurological, and so on

Use quotes to search for an exact phrase

"primary prevention of cancer"
(heart or cardiac or cardio*) AND arrest -"American Heart Association"