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https://www.readbyqxmd.com/read/28555142/oligodeoxynucleotides-can-transiently-up-and-downregulate-chs-gene-expression-in-flax-by-changing-dna-methylation-in-a-sequence-specific-manner
#1
Magdalena Dzialo, Jan Szopa, Tadeusz Czuj, Magdalena Zuk
Chalcone synthase (CHS) has been recognized as an essential enzyme in the phenylpropanoid biosynthesis pathway. Apart from the leading role in the production of phenolic compounds with many valuable biological activities beneficial to biomedicine, CHS is well appreciated in science. Genetic engineering greatly facilitates expanding knowledge on the function and genetics of CHS in plants. The CHS gene is one of the most intensively studied genes in flax. In our study, we investigated engineering of the CHS gene through genetic and epigenetic approaches...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28529764/in-vivo-analysis-of-covering-materials-composed-of-biodegradable-polymers-enriched-with-flax-fibers
#2
Tomasz Gredes, Sandra Schönitz, Tomasz Gedrange, Lukas Stepien, Karol Kozak, Christiane Kunert-Keil
BACKGROUND: The objective of this study was to investigate the in vivo effect of bioactive composites with poly(lactic acid) (PLA) or polycaprolactone (PCL) as the matrix, reinforced with bioplastic flax fibers, on the surrounding muscle tissue. METHODS: Materials of pure PLA and PCL and their composites with flax fibers from genetically modified plants producing poly-3-hydroxybutyrate (PLA-transgen, PCL-transgen) and unmodified plants (PLA-wt, PCL-wt) were placed subcutaneous on the M...
2017: Biomaterials Research
https://www.readbyqxmd.com/read/28488060/arbuscular-mycorrhizal-fungi-and-pseudomonas-in-reduce-drought-stress-damage-in-flax-linum-usitatissimum-l-a-field-study
#3
Saeedeh Rahimzadeh, Alireza Pirzad
Drought stress, which is one of the most serious world environmental threats to crop production, might be compensated by some free living and symbiotic soil microorganisms. The physiological response of flax plants to inoculation with two species of arbuscular mycorrhizal (AM) fungi (Funneliformis mosseae or Rhizophagus intraradices) and a phosphate solubilizing bacterium (Pseudomonas putida P13; PSB) was evaluated under different irrigation regimes (irrigation after 60, 120, and 180 mm of evaporation from Class A pan as well-watered, mild, and severe stress, respectively)...
May 10, 2017: Mycorrhiza
https://www.readbyqxmd.com/read/28451749/functional-characterization-of-the-pinoresinol-lariciresinol-reductase-2-gene-reveals-its-roles-in-yatein-biosynthesis-and-flax-defense-response
#4
Cyrielle Corbin, Samantha Drouet, Ivan Mateljak, Lucija Markulin, Cédric Decourtil, Sullivan Renouard, Tatiana Lopez, Joël Doussot, Frédéric Lamblin, Daniel Auguin, Eric Lainé, Elisabeth Fuss, Christophe Hano
This study provides new insights into the biosynthesis regulation and in planta function of the lignan yatein in flax leaves. Pinoresinol-lariciresinol reductases (PLR) catalyze the conversion of pinoresinol into secoisolariciresinol (SECO) in lignan biosynthesis. Several lignans are accumulated in high concentrations, such as SECO accumulated as secoisolariciresinol diglucoside (SDG) in seeds and yatein in aerial parts, in the flax plant (Linum usitatissimum L.) from which two PLR enzymes of opposite enantioselectivity have been isolated...
April 27, 2017: Planta
https://www.readbyqxmd.com/read/28447607/genome-wide-identification-characterization-and-expression-profile-of-aquaporin-gene-family-in-flax-linum-usitatissimum
#5
S M Shivaraj, Rupesh K Deshmukh, Rhitu Rai, Richard Bélanger, Pawan K Agrawal, Prasanta K Dash
Membrane intrinsic proteins (MIPs) form transmembrane channels and facilitate transport of myriad substrates across the cell membrane in many organisms. Majority of plant MIPs have water transporting ability and are commonly referred as aquaporins (AQPs). In the present study, we identified aquaporin coding genes in flax by genome-wide analysis, their structure, function and expression pattern by pan-genome exploration. Cross-genera phylogenetic analysis with known aquaporins from rice, arabidopsis, and poplar showed five subgroups of flax aquaporins representing 16 plasma membrane intrinsic proteins (PIPs), 17 tonoplast intrinsic proteins (TIPs), 13 NOD26-like intrinsic proteins (NIPs), 2 small basic intrinsic proteins (SIPs), and 3 uncharacterized intrinsic proteins (XIPs)...
April 27, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28314980/ex-post-assessment-of-genetically-modified-low-level-presence-in-canadian-flax
#6
Helen M Booker, Eric G Lamb, Stuart J Smyth
Canada is the world's largest producer and exporter of flaxseed. In 2009, DNA from deregistered genetically modified (GM) CDC Triffid was detected in a shipment of Canadian flaxseed exported to Europe, causing a large decrease in the amount of flax planted in Canada and a major shift in export markets. The flax industry in Canada undertook major changes to ensure the removal of transgenic flax from the supply chain. To demonstrate compliance, Canada adopted a protocol involving testing grain samples (post-harvest) using an RT-PCR test for the construct found in CDC Triffid...
June 2017: Transgenic Research
https://www.readbyqxmd.com/read/28299328/mir319-mir390-and-mir393-are-involved-in-aluminum-response-in-flax-linum-usitatissimum-l
#7
Alexey A Dmitriev, Anna V Kudryavtseva, Nadezhda L Bolsheva, Alexander V Zyablitsin, Tatiana A Rozhmina, Natalya V Kishlyan, George S Krasnov, Anna S Speranskaya, Anastasia A Krinitsina, Asiya F Sadritdinova, Anastasiya V Snezhkina, Maria S Fedorova, Olga Yu Yurkevich, Olga V Muravenko, Maxim S Belenikin, Nataliya V Melnikova
Acid soils limit agricultural production worldwide. Major reason of crop losses in acid soils is the toxicity of aluminum (Al). In the present work, we investigated expression alterations of microRNAs in flax (Linum usitatissimum L.) plants under Al stress. Flax seedlings of resistant (TMP1919 and G1071/4_k) and sensitive (Lira and G1071/4_o) to Al cultivars and lines were exposed to AlCl3 solution for 4 and 24 hours. Twelve small RNA libraries were constructed and sequenced using Illumina platform. In total, 97 microRNAs from 18 conserved families were identified...
2017: BioMed Research International
https://www.readbyqxmd.com/read/28277078/the-use-of-phosphomannose-isomerase-selection-system-for-agrobacterium-mediated-transformation-of-tobacco-and-flax-aimed-for-phytoremediation
#8
Jitka Hilgert, Martina Sura-De Jong, Jiří Fišer, Kateřina Tupá, Miroslava Vrbová, Miroslav Griga, Tomáš Macek, Jana Žiarovská
A plant selection system based on the phosphomannose isomerase gene (pmi) as a selectable marker is often used to avoid selection using antibiotic resistance. Nevertheless, pmi gene is endogenous in several plant species and therefore difficult to use in such cases. Here we evaluated and compared Agrobacterium-mediated transformation of Linum usitatissimum breeding line AGT-952 (without endogenous pmi gene) and Nicotiana tabacum var. WSC-38 (with endogenous pmi gene). Transformation was evaluated for vectors bearing transgenes that have the potential to be involved in improved phytoremediation of contaminated environment...
May 4, 2017: Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes
https://www.readbyqxmd.com/read/28262560/bliss-a-bioorthogonal-dual-labeling-strategy-to-unravel-lignification-dynamics-in-plants
#9
Cedric Lion, Clémence Simon, Brigitte Huss, Anne-Sophie Blervacq, Louis Tirot, Djadidi Toybou, Corentin Spriet, Christian Slomianny, Yann Guerardel, Simon Hawkins, Christophe Biot
A better in vivo understanding of lignin formation within plant cell walls will contribute to improving the valorization of plant-derived biomass. Although bioorthogonal chemistry provides a promising platform to study the lignification process, methodologies that simultaneously detect multiple chemical reporters in living organisms are still scarce. Here, we have developed an original bioorthogonal labeling imaging sequential strategy (BLISS) to visualize and analyze the incorporation of both p-hydroxyphenyl (H) and guaiacyl (G) units into lignin in vivo with a combination of strain-promoted and copper-catalyzed azide-alkyne cycloadditions...
March 16, 2017: Cell Chemical Biology
https://www.readbyqxmd.com/read/28188068/ssr-and-morphological-trait-based-population-structure-analysis-of-130-diverse-flax-linum-usitatissimum-l-accessions
#10
Shashi Bhushan Choudhary, Hariom Kumar Sharma, Arroju Anil Kumar, Rangappa Thimmaiah Maruthi, Jiban Mitra, Isholeena Chowdhury, Binay Kumar Singh, Pran Gobinda Karmakar
A total of 130 flax accessions of diverse morphotypes and worldwide origin were assessed for genetic diversity and population structure using 11 morphological traits and microsatellite markers (15 gSSRs and 7 EST-SSRs). Analysis performed after classifying these accessions on the basis of plant height, branching pattern, seed size, Indian/foreign origin into six categories called sub-populations viz. fibre type exotic, fibre type indigenous, intermediate type exotic, intermediate type indigenous, linseed type exotic and linseed type indigenous...
February 2017: Comptes Rendus Biologies
https://www.readbyqxmd.com/read/28159890/multiple-functional-self-association-interfaces-in-plant-tir-domains
#11
Xiaoxiao Zhang, Maud Bernoux, Adam R Bentham, Toby E Newman, Thomas Ve, Lachlan W Casey, Tom M Raaymakers, Jian Hu, Tristan I Croll, Karl J Schreiber, Brian J Staskawicz, Peter A Anderson, Kee Hoon Sohn, Simon J Williams, Peter N Dodds, Bostjan Kobe
The self-association of Toll/interleukin-1 receptor/resistance protein (TIR) domains has been implicated in signaling in plant and animal immunity receptors. Structure-based studies identified different TIR-domain dimerization interfaces required for signaling of the plant nucleotide-binding oligomerization domain-like receptors (NLRs) L6 from flax and disease resistance protein RPS4 from Arabidopsis Here we show that the crystal structure of the TIR domain from the Arabidopsis NLR suppressor of npr1-1, constitutive 1 (SNC1) contains both an L6-like interface involving helices αD and αE (DE interface) and an RPS4-like interface involving helices αA and αE (AE interface)...
March 7, 2017: Proceedings of the National Academy of Sciences of the United States of America
https://www.readbyqxmd.com/read/28118137/effect-of-source-of-dna-on-the-quantitative-analysis-of-genetically-engineered-traits-using-digital-pcr-and-real-time-pcr
#12
Tigst Demeke, Jemima Malabanan, Michelle Holigroski, Monika Eng
Seven commercially available DNA extraction kits were compared with a cetyltrimethylammonium bromide (CTAB) method to determine the suitability of the extracted DNA for RainDrop digital PCR (dPCR) and real-time PCR (RT-PCR) quantification of OXY235 canola, FP967 flax, and DP305423 soybean (spiked at the 0.1% level). For the kits, the highest amount of DNA extracted from a 0.2 g sample was obtained using OmniPrep for Plant for flax and DNeasy mericon Food for canola and soybean. For canola, DNA extracted with the Fast ID Genomic DNA Extraction Kit, FastDNA Spin Kit, GM Quicker 2, NucleoSpin Food, and DNeasy mericon Food was suitable for dPCR and RT-PCR...
March 1, 2017: Journal of AOAC International
https://www.readbyqxmd.com/read/28105944/gene-expression-profiling-of-flax-linum-usitatissimum-l-under-edaphic-stress
#13
Alexey A Dmitriev, Anna V Kudryavtseva, George S Krasnov, Nadezhda V Koroban, Anna S Speranskaya, Anastasia A Krinitsina, Maxim S Belenikin, Anastasiya V Snezhkina, Asiya F Sadritdinova, Natalya V Kishlyan, Tatiana A Rozhmina, Olga Yu Yurkevich, Olga V Muravenko, Nadezhda L Bolsheva, Nataliya V Melnikova
BACKGROUND: Cultivated flax (Linum usitatissimum L.) is widely used for production of textile, food, chemical and pharmaceutical products. However, various stresses decrease flax production. Search for genes, which are involved in stress response, is necessary for breeding of adaptive cultivars. Imbalanced concentration of nutrient elements in soil decrease flax yields and also results in heritable changes in some flax lines. The appearance of Linum Insertion Sequence 1 (LIS-1) is the most studied modification...
November 16, 2016: BMC Plant Biology
https://www.readbyqxmd.com/read/28024547/metrics-of-rhamnogalacturonan-i-with-%C3%AE-1%C3%A2-4-linked-galactan-side-chains-and-structural-basis-for-its-self-aggregation
#14
Olga N Makshakova, Tatyana A Gorshkova, Polina V Mikshina, Yuriy F Zuev, Serge Perez
Within the family of plant cell wall polysaccharides rhamnogalacturonans I are the most diverse and structurally complex members. In present study we characterize the 3-dimensional structures and dynamic features of the constituents of RG-I along MD trajectories. It is demonstrated that extended threefold helical structure of the rhamnogalacturonan linear backbone is the most energetically favorable motif. Branching helps to stabilize a conformer of the backbone twisted along 1→2 glycosidic linkage triggering the orientation of long side chains without altering the extended overall backbone chain conformation...
February 20, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/28008335/structure-and-function-of-the-tir-domain-from-the-grape-nlr-protein-rpv1
#15
Simon J Williams, Ling Yin, Gabriel Foley, Lachlan W Casey, Megan A Outram, Daniel J Ericsson, Jiang Lu, Mikael Boden, Ian B Dry, Bostjan Kobe
The N-terminal Toll/interleukin-1 receptor/resistance protein (TIR) domain has been shown to be both necessary and sufficient for defense signaling in the model plants flax and Arabidopsis. In examples from these organisms, TIR domain self-association is required for signaling function, albeit through distinct interfaces. Here, we investigate these properties in the TIR domain containing resistance protein RPV1 from the wild grapevine Muscadinia rotundifolia. The RPV1 TIR domain, without additional flanking sequence present, is autoactive when transiently expressed in tobacco, demonstrating that the TIR domain alone is capable of cell-death signaling...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27987846/differential-growth-of-bowel-commensal-bacteroides-species-on-plant-xylans-of-differing-structural-complexity
#16
Manuela Centanni, Jennifer C Hutchison, Susan M Carnachan, Alison M Daines, William J Kelly, Gerald W Tannock, Ian M Sims
Alterations to the composition of the bowel microbiota (dysbioses) are associated with particular diseases and conditions of humans. There is a need to discover new, indigestible polysaccharides which are selective growth substrates for commensal bowel bacteria. These substrates (prebiotics) could be added to food in intervention studies to correct bowel dysbiosis. A collection of commensal bacteria was screened for growth in culture using a highly-branched xylan produced by New Zealand flax. Two, Bacteroides ovatus ATCC 8483 and Bacteroides xylanisolvens DSM 18836 grew well on this substrate...
February 10, 2017: Carbohydrate Polymers
https://www.readbyqxmd.com/read/27981388/transcriptome-portrait-of-cellulose-enriched-flax-fibres-at-advanced-stage-of-specialization
#17
Oleg Gorshkov, Natalia Mokshina, Vladimir Gorshkov, Svetlana Chemikosova, Yuri Gogolev, Tatyana Gorshkova
Functional specialization of cells is among the most fundamental processes of higher organism ontogenesis. The major obstacle to studying this phenomenon in plants is the difficulty of isolating certain types of cells at defined stages of in planta development for in-depth analysis. A rare opportunity is given by the developed model system of flax (Linum usitatissimum L.) phloem fibres that can be purified from the surrounding tissues at the stage of the tertiary cell wall deposition. The performed comparison of the whole transcriptome profile in isolated fibres and other portions of the flax stem, together with fibre metabolism characterization, helped to elucidate the general picture of the advanced stage of plant cell specialization and to reveal novel participants potentially involved in fibre metabolism regulation and cell wall formation...
March 2017: Plant Molecular Biology
https://www.readbyqxmd.com/read/27933082/rna-seq-transcriptome-response-of-flax-linum-usitatissimum-l-to-the-pathogenic-fungus-fusarium-oxysporum-f-sp-lini
#18
Leonardo Galindo-González, Michael K Deyholos
Fusarium oxysporum f. sp. lini is a hemibiotrophic fungus that causes wilt in flax. Along with rust, fusarium wilt has become an important factor in flax production worldwide. Resistant flax cultivars have been used to manage the disease, but the resistance varies, depending on the interactions between specific cultivars and isolates of the pathogen. This interaction has a strong molecular basis, but no genomic information is available on how the plant responds to attempted infection, to inform breeding programs on potential candidate genes to evaluate or improve resistance across cultivars...
2016: Frontiers in Plant Science
https://www.readbyqxmd.com/read/27776375/investigation-of-the-lignan-content-in-extracts-from-linum-callitris-and-juniperus-species-in-relation-to-their-in-vitro-antiproliferative-activities
#19
Joël Doussot, Véronique Mathieu, Cyril Colas, Roland Molinié, Cyrielle Corbin, Josiane Montguillon, Laeticia Moreno Y Banuls, Sullivan Renouard, Frédéric Lamblin, Patricia Dupré, Benoit Maunit, Robert Kiss, Christophe Hano, Eric Lainé
Podophyllotoxin, a lignan still extracted from the rhizomes of Podophyllum hexandrum (Berberidaceae), is the starting molecule for the semisynthesis of widely used anticancer drugs such as etoposide. However, this source is threatened by the over-collection of P. hexandrum. Plants belonging to the Linaceae and Cupressaceae families could be attractive alternative sources with species that contain the lignan podophyllotoxin or its precursors and derivatives. Wild flax species, such as Linum flavum, as well as some Juniperus and Callitris species were investigated for their lignan content, and the in vitro antiproliferative capacity of their extracts was assayed on four tumor cell lines...
April 2017: Planta Medica
https://www.readbyqxmd.com/read/27626291/synthesis-and-characterization-of-site-selective-orbitide-bsa-conjugate-to-produce-antibodies
#20
Pramodkumar D Jadhav, Youn Young Shim, Martin J T Reaney
Bioactive flax cyclic peptides (orbitides and linusorbs) were site-specifically ligated through methionine with bovine serum albumin (BSA) to produce immunogenic compounds. In this study, modified flaxseed immunosuppressant orbitides (linusorbs or LOs) containing hydroxyl (OH) groups were synthesized for use as haptens. These compounds were extensively characterized by (1)H nuclear magnetic resonance (NMR), (13)C NMR, high-performance liquid chromatography-tandem mass spectrometry, and Fourier transform infrared spectroscopy...
October 19, 2016: Bioconjugate Chemistry
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