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https://www.readbyqxmd.com/read/29761434/untargeted-analysis-of-semipolar-compounds-by-lc-ms-and-targeted-analysis-of-fatty-acids-by-gc-ms-gc-fid-from-plant-cultivation-to-extract-preparation
#1
Camille Bénard, Sébastien Acket, Yannick Rossez, Olivier Fernandez, Thierry Berton, Yves Gibon, Cécile Cabasson
The way plants are grown and samples are harvested, prepared, and extracted has a profound impact on the output of a metabolomics experiment. In this chapter, we detail the experimental procedures from plant cultivation to extract preparation, in order to avoid difficulties that could result in contamination or undesired changes of the analytes. Two plant organs are mentioned as examples: tomato fruits (Solanum lycopersicum) and flax seeds (Linum usitatissimum). Extractions designed for the untargeted analysis of semipolar compounds by liquid chromatography-mass spectrometry (LC-MS) and targeted analysis of fatty acids by gas chromatography-mass spectrometry (GC-MS) or gas chromatography with flame ionization detector (GC-FID) are described...
2018: Methods in Molecular Biology
https://www.readbyqxmd.com/read/29713868/a-genome-wide-analysis-of-the-flax-linum-usitatissimum-l-dirigent-protein-family-from-gene-identification-and-evolution-to-differential-regulation
#2
Cyrielle Corbin, Samantha Drouet, Lucija Markulin, Daniel Auguin, Éric Lainé, Laurence B Davin, John R Cort, Norman G Lewis, Christophe Hano
Identification of DIR encoding genes in flax genome. Analysis of phylogeny, gene/protein structures and evolution. Identification of new conserved motifs linked to biochemical functions. Investigation of spatio-temporal gene expression and response to stress. Dirigent proteins (DIRs) were discovered during 8-8' lignan biosynthesis studies, through identification of stereoselective coupling to afford either (+)- or (-)-pinoresinols from E-coniferyl alcohol. DIRs are also involved or potentially involved in terpenoid, allyl/propenyl phenol lignan, pterocarpan and lignin biosynthesis...
April 30, 2018: Plant Molecular Biology
https://www.readbyqxmd.com/read/29691017/spatial-structures-of-rhamnogalacturonan-i-in-gel-and-colloidal-solution-identified-by-1d-and-2d-ftir-spectroscopy
#3
Olga N Makshakova, Dzhigangir A Faizullin, Polina V Mikshina, Tatyana A Gorshkova, Yuriy F Zuev
Rhamnogalacturonan I (RG-I), a polysaccharide found in different types of plant cell walls, fulfills specific functions, the structural basis of which remains unclear. Generalized 2D correlation FTIR spectroscopy with dehydration was employed to reveal the structure and interactions in flax RG-I solution and microwave treated gel. Varying water content allowed emphasizing a role of solvent in maintaining different structures. In the gel, 2D correlation maps prove the existence of a conformationally uniform highly hydrated structure...
July 15, 2018: Carbohydrate Polymers
https://www.readbyqxmd.com/read/29670584/comparative-microbiome-analysis-of-a-fusarium-wilt-suppressive-soil-and-a-fusarium-wilt-conducive-soil-from-the-ch%C3%A3-teaurenard-region
#4
Katarzyna Siegel-Hertz, Véronique Edel-Hermann, Emilie Chapelle, Sébastien Terrat, Jos M Raaijmakers, Christian Steinberg
Disease-suppressive soils are soils in which specific soil-borne plant pathogens cause only limited disease although the pathogen and susceptible host plants are both present. Suppressiveness is in most cases of microbial origin. We conducted a comparative metabarcoding analysis of the taxonomic diversity of fungal and bacterial communities from suppressive and non-suppressive (conducive) soils as regards Fusarium wilts sampled from the Châteaurenard region (France). Bioassays based on Fusarium wilt of flax confirmed that disease incidence was significantly lower in the suppressive soil than in the conducive soil...
2018: Frontiers in Microbiology
https://www.readbyqxmd.com/read/29580528/sorting-out-the-phytoprostane-and-phytofuran-profile-in-vegetable-oils
#5
Raúl Domínguez-Perles, Ángel Abellán, Daniel León, Federico Ferreres, Alexander Guy, Camille Oger, Jean Marie Galano, Thierry Durand, Ángel Gil-Izquierdo
Phytoprostanes (PhytoPs) and phytofurans (PhytoFs) are prostaglandin-like compounds, contributing to defense signaling and prevention of cellular damage. These plant oxylipins result from autoxidation of α-linolenic acid (ALA) and have been proposed as new bioactive compounds due to their structural analogies with isoprostanes (IsoPs) and prostanoids derived from arachidonic acid in mammals, which have demonstrated diverse biological activities. The present work assesses a wide range of vegetable oils - including extra virgin olive oils (n = 7) and flax, sesame, argan, safflower seed, grapeseed, and palm oils - for their content of PhytoPs and PhytoFs...
May 2018: Food Research International
https://www.readbyqxmd.com/read/29579641/expression-of-heterologous-lycopene-%C3%AE-cyclase-gene-in-flax-can-cause-silencing-of-its-endogenous-counterpart-by-changes-in-gene-body-methylation-and-in-aba-homeostasis-mechanism
#6
Aleksandra Boba, Kamil Kostyn, Marta Preisner, Wioleta Wojtasik, Jan Szopa, Anna Kulma
Previously we described flax plants with expression of Arabidopsis lycopene β-cyclase (lcb) gene in which decreased expression of the endogenous lcb and increased resistance to fungal pathogen was observed. We suggested that co-suppression was responsible for the change. In this study we investigated the molecular basis of the observed effect in detail. We found that methylation changes in the Lulcb gene body might be responsible for repression of the gene. Treatment with azacitidine (DNA methylation inhibitor) confirmed the results...
March 21, 2018: Plant Physiology and Biochemistry: PPB
https://www.readbyqxmd.com/read/29452103/analysis-of-13-c-labeling-amino-acids-by-capillary-electrophoresis-high-resolution-mass-spectrometry-in-developing-flaxseed
#7
Sébastien Acket, Anthony Degournay, Mathilde Gosset, Franck Merlier, Manual Adrian Troncoso-Ponce, Brigitte Thomasset
In context of fluxomic studies, 13 C labeling analysis of amino acids are very important for solving the carbon flow calculation, because they are synthesized in various biosynthesis pathways and cellular compartments in plant cells. Traditionally, 13 C labeling analysis are performed using low resolution mass spectrometry detector by GC-MS. We compared a method using capillary electrophoresis-high resolution mass spectrometry without derivatization and with better accuracy assessment of labeling measurements comparing to classical GC-MS...
April 15, 2018: Analytical Biochemistry
https://www.readbyqxmd.com/read/29381737/identification-and-functional-analysis-of-two-alternatively-spliced-transcripts-of-abscisic-acid-insensitive3-abi3-in-linseed-flax-linum-usitatissimum-l
#8
Yanyan Wang, Tianbao Zhang, Xiaxia Song, Jianping Zhang, Zhanhai Dang, Xinwu Pei, Yan Long
Alternative splicing is a popular phenomenon in different types of plants. It can produce alternative spliced transcripts that encode proteins with altered functions. Previous studies have shown that one transcription factor, ABSCISIC ACID INSENSITIVE3 (ABI3), which encodes an important component in abscisic acid (ABA) signaling, is subjected to alternative splicing in both mono- and dicotyledons. In the current study, we identified two homologs of ABI3 in the genome of linseed flax. We screened two alternatively spliced flax LuABI3 transcripts, LuABI3-2 and LuABI3-3, and one normal flax LuABI3 transcript, LuABI3-1...
2018: PloS One
https://www.readbyqxmd.com/read/29358834/quality-assessment-of-low-sugar-jams-enriched-with-plant-raw-materials-exhibiting-health-promoting-properties
#9
Anna Banaś, Anna Korus, Małgorzata Tabaszewska
Low-sugar gooseberry jams enriched by the addition of black chokeberry, elderberry, Japanese quince, flax seeds and wheat germs were examined for the content of total polyphenols, total flavonoids, and total anthocyanins as well as their antioxidant activity (DPPH, ABTS, and FRAP) and individual phenolic compounds. The jams were evaluated immediately after production and after 6 and 12 months of storage. Samples were stored at chilled temperature (10 °C) and room temperature (20 °C). A significant increase in the level of the analyzed components and antioxidant activity were determined in jams with the addition of chokeberry, elderberry and Japanese quince, while in the case of other plant ingredients the differences were not always significant...
January 2018: Journal of Food Science and Technology
https://www.readbyqxmd.com/read/29297347/differential-gene-expression-in-response-to-fusarium-oxysporum-infection-in-resistant-and-susceptible-genotypes-of-flax-linum-usitatissimum-l
#10
Alexey A Dmitriev, George S Krasnov, Tatiana A Rozhmina, Roman O Novakovskiy, Anastasiya V Snezhkina, Maria S Fedorova, Olga Yu Yurkevich, Olga V Muravenko, Nadezhda L Bolsheva, Anna V Kudryavtseva, Nataliya V Melnikova
BACKGROUND: Flax (Linum usitatissimum L.) is a crop plant used for fiber and oil production. Although potentially high-yielding flax varieties have been developed, environmental stresses markedly decrease flax production. Among biotic stresses, Fusarium oxysporum f. sp. lini is recognized as one of the most devastating flax pathogens. It causes wilt disease that is one of the major limiting factors for flax production worldwide. Breeding and cultivation of flax varieties resistant to F...
December 28, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/29277026/the-cinnamyl-alcohol-dehydrogenase-family-in-flax-differentiation-during-plant-growth-and-under-stress-conditions
#11
Marta Preisner, Wioleta Wojtasik, Kamil Kostyn, Aleksandra Boba, Tadeusz Czuj, Jan Szopa, Anna Kulma
Cinnamyl alcohol dehydrogenase (CAD), which catalyzes the reduction of cinnamaldehydes to their alcohol derivatives, is represented by a large family of proteins. The aim of the study was to identify the CAD isoforms in flax (Linum usitatissimum L.) - LuCADs - and to determine their specificity to enhance knowledge of the mechanisms controlling cell wall lignification in flax under environmental stresses. On the basis of genome-wide analysis, we identified 15 isoforms (one in two copies) belonging to three major classes of the CAD protein family...
February 2018: Journal of Plant Physiology
https://www.readbyqxmd.com/read/29221437/evolutionary-characterization-and-transcript-profiling-of-%C3%AE-tubulin-genes-in-flax-linum-usitatissimum-l-during-plant-development
#12
Floriana Gavazzi, Gaia Pigna, Luca Braglia, Silvia Gianì, Diego Breviario, Laura Morello
BACKGROUND: Microtubules, polymerized from alpha and beta-tubulin monomers, play a fundamental role in plant morphogenesis, determining the cell division plane, the direction of cell expansion and the deposition of cell wall material. During polarized pollen tube elongation, microtubules serve as tracks for vesicular transport and deposition of proteins/lipids at the tip membrane. Such functions are controlled by cortical microtubule arrays. Aim of this study was to first characterize the flax β-tubulin family by sequence and phylogenetic analysis and to investigate differential expression of β-tubulin genes possibly related to fibre elongation and to flower development...
December 8, 2017: BMC Plant Biology
https://www.readbyqxmd.com/read/29104570/characterization-of-bacterial-and-fungal-community-dynamics-by-high-throughput-sequencing-hts-metabarcoding-during-flax-dew-retting
#13
Christophe Djemiel, Sébastien Grec, Simon Hawkins
Flax dew-retting is a key step in the industrial extraction of fibers from flax stems and is dependent upon the production of a battery of hydrolytic enzymes produced by micro-organisms during this process. To explore the diversity and dynamics of bacterial and fungal communities involved in this process we applied a high-throughput sequencing (HTS) DNA metabarcoding approach (16S rRNA/ITS region, Illumina Miseq) on plant and soil samples obtained over a period of 7 weeks in July and August 2014. Twenty-three bacterial and six fungal phyla were identified in soil samples and 11 bacterial and four fungal phyla in plant samples...
2017: Frontiers in Microbiology
https://www.readbyqxmd.com/read/28993783/genetic-variability-of-27-traits-in-a-core-collection-of-flax-linum-usitatissimum-l
#14
Frank M You, Gaofeng Jia, Jin Xiao, Scott D Duguid, Khalid Y Rashid, Helen M Booker, Sylvie Cloutier
Assessment of genetic variability of plant core germplasm is needed for efficient germplasm utilization in breeding improvement. A total of 391 accessions of a flax core collection, which preserves the variation present in the world collection of 3,378 accessions maintained by Plant Gene Resources of Canada (PGRC) and represents a broad range of geographical origins, different improvement statuses and two morphotypes, was evaluated in field trials in up to 8 year-location environments for 10 agronomic, eight seed quality, six fiber and three disease resistance traits...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28982196/effect-of-mechanical-damage-and-wound-healing-on-the-viscoelastic-properties-of-stems-of-flax-cultivars-linum-usitatissimum-l-cv-eden-and-cv-drakkar
#15
Cloé Paul-Victor, Sara Dalle Vacche, Federica Sordo, Siegfried Fink, Thomas Speck, Véronique Michaud, Olga Speck
As plant fibres are increasingly used in technical textiles and their composites, underlying principles of wound healing in living plant fibres are relevant to product quality, and provide inspiration for biomimetic healing in synthetic materials. In this work, two Linum usitatissimum cultivars differing in their stem mechanical properties, cv. Eden (stems resistant to lodging) and cv. Drakkar (with more flexible stems), were grown without wound or with stems previously wounded with a cut parallel or transversal to the stem...
2017: PloS One
https://www.readbyqxmd.com/read/28894144/molecular-details-of-secretory-phospholipase-a2-from-flax-linum-usitatissimum-l-provide-insight-into-its-structure-and-function
#16
Payal Gupta, Prasanta K Dash
Secretory phospholipase A2 (sPLA2) are low molecular weight proteins (12-18 kDa) involved in a suite of plant cellular processes imparting growth and development. With myriad roles in physiological and biochemical processes in plants, detailed analysis of sPLA2 in flax/linseed is meagre. The present work, first in flax, embodies cloning, expression, purification and molecular characterisation of two distinct sPLA2s (I and II) from flax. PLA2 activity of the cloned sPLA2s were biochemically assayed authenticating them as bona fide phospholipase A2...
September 11, 2017: Scientific Reports
https://www.readbyqxmd.com/read/28878799/integration-of-physical-genetic-and-cytogenetic-mapping-data-for-cellulose-synthase-cesa-genes-in-flax-linum-usitatissimum-l
#17
Olga Y Yurkevich, Ilya V Kirov, Nadezhda L Bolsheva, Olga A Rachinskaya, Zoya E Grushetskaya, Svyatoslav A Zoschuk, Tatiana E Samatadze, Marina V Bogdanova, Valentina A Lemesh, Alexandra V Amosova, Olga V Muravenko
Flax, Linum usitatissimum L., is a valuable multi-purpose plant, and currently, its genome is being extensively investigated. Nevertheless, mapping of genes in flax genome is still remaining a challenging task. The cellulose synthase (CesA) multigene family involving in the process of cellulose synthesis is especially important for metabolism of this fiber crop. For the first time, fluorescent in situ hybridization (FISH)-based chromosomal localization of the CesA conserved fragment (KF011584.1), 5S, and 26S rRNA genes was performed in landrace, oilseed, and fiber varieties of L...
2017: Frontiers in Plant Science
https://www.readbyqxmd.com/read/28817232/crystal-structure-of-the-melampsora-lini-effector-avrp-reveals-insights-into-a-possible-nuclear-function-and-recognition-by-the-flax-disease-resistance-protein-p
#18
Xiaoxiao Zhang, Nadya Farah, Laura Rolston, Daniel J Ericsson, Ann-Maree Catanzariti, Maud Bernoux, Thomas Ve, Katerina Bendak, Chunhong Chen, Joel P Mackay, Gregory J Lawrence, Adrienne Hardham, Jeffrey G Ellis, Simon J Williams, Peter N Dodds, David A Jones, Bostjan Kobe
The effector protein AvrP is secreted by the flax rust fungal pathogen (Melampsora lini) and recognized specifically by the flax (Linum usitatissimum) P disease resistance protein, leading to effector-triggered immunity. To investigate the biological function of this effector and mechanisms of specific recognition by the P resistance protein, we determined the crystal structure of AvrP. The structure reveals an elongated zinc-finger-like structure with a novel interleaved zinc-binding topology. The residues responsible for zinc binding are conserved in AvrP effector variants and mutations of these motifs result in loss of P-mediated recognition...
August 17, 2017: Molecular Plant Pathology
https://www.readbyqxmd.com/read/28792104/expression-analysis-of-cellulose-synthase-and-main-cytoskeletal-protein-genes-in-flax-linum-usitatissimum-l
#19
Dmitry Galinousky, Tsimafei Padvitski, Galina Bayer, Yaroslav Pirko, Nikolay Pydiura, Natallia Anisimova, Tatyana Nikitinskaya, Liubov Khotyleva, Alla Yemets, Aleksandr Kilchevsky, Yaroslav Blume
Fiber flax is an important source of natural fiber and a comprehensive model for the plant fiber biogenesis studies. Cellulose-synthase (CesA) and cytoskeletal genes are known to be important for the cell wall biogenesis in general and for the biogenesis of flax fibers in particular. Currently, knowledge about activity of these genes during the plant growth is limited. In this study, we have investigated flax fiber biogenesis by measuring expression of CesA and cytoskeletal genes at two stages of the flax development (seedlings and stems at the rapid growth stage) in several flax subspecies (elongatum, mediterraneum, crepitans)...
August 9, 2017: Cell Biology International
https://www.readbyqxmd.com/read/28705193/spatial-regulation-of-monolignol-biosynthesis-and-laccase-genes-control-developmental-and-stress-related-lignin-in-flax
#20
Julien Le Roy, Anne-Sophie Blervacq, Anne Créach, Brigitte Huss, Simon Hawkins, Godfrey Neutelings
BACKGROUND: Bast fibres are characterized by very thick secondary cell walls containing high amounts of cellulose and low lignin contents in contrast to the heavily lignified cell walls typically found in the xylem tissues. To improve the quality of the fiber-based products in the future, a thorough understanding of the main cell wall polymer biosynthetic pathways is required. In this study we have carried out a characterization of the genes involved in lignin biosynthesis in flax along with some of their regulation mechanisms...
July 14, 2017: BMC Plant Biology
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