Elisabetta Caon, Maria Martins, Harry Hodgetts, Lieke Blanken, Maria Giovanna Vilia, Ana Levi, Kessarin Thanapirom, Walid Al-Akkad, Jeries Abu-Hanna, Guido Baselli, Andrew R Hall, Tu Vinh Luong, Jan-Willem Taanman, Michele Vacca, Luca Valenti, Stefano Romeo, Giuseppe Mazza, Massimo Pinzani, Krista Rombouts
BACKGROUND AND AIMS: The PNPLA3 rs738409 C>G (encoding for I148M) variant is a risk locus for the fibrogenic progression of chronic liver diseases, a process driven by hepatic stellate cells (HSCs). We investigated how the PNPLA3 I148M variant affects HSCs biology using transcriptomic data and validated findings in 3D-culture models. METHODS: RNA sequencing was performed on 2D-cultured primary human HSCs and liver biopsies of obese individuals, genotyped for the PNPLA3 I148M variant...
February 14, 2024: Journal of Hepatology